NOSAI NOSAI

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つかさくぬぎ
5 years ago
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1 NOSAI NOSAI NOSAI NOSAI NOSAI NOSAI NOSAI NOSAI NOSAI NOSAI

2 NOSAI NOSAI

3 NOSAI NOSAI NOSAI FSFS FS FS FS Salmonella Typhimurium ST Salmonella Dublin SD SD ST ST

4 NOSAI S. Aberdeen TMR χ S. Aberdeen O11 i S.Aberdeen PP Salmonella P S. Aberdeen O4 SalmonellaO4 S Salmonella TyphimuriumST Salmonella sp. O4 ioi O4 i ST O4 S O S O4 SST O4 i O4 i ST PCR Xba PFGE multiple-locus variable number tandem repeat analysismlva O4 i ST H2 fljab-hin PFGE MLVA O4 S ST O4 i O4 i ST H2 ST ST O4 S O4 S

5 NOSAI Salmonella O4i Salmonella Typhimurium ST ST Salmonella. Dublin LPS ELISA ELISA ST Salmonella. Dublin O4i NOSAI Salmonella enterica O4 i O4 i Salmonella enterica Typhimurium ST ST O4 i O4 i O4 i O4 i O4 i

6 NOSAI LPS LPS B S A G TP AST CPK NOSAI NOSAI NOSAI NOSAI NOSAI OM Mycoplasma bovis M.bovis OM kg TS OM M.bovis Pasteurella µl g dl g dl DGkg M.bovis DG Ht OM OM OM M.bovis DG A G

7 NOSAI NOSAI NOSAI NOSAI NOSAI OM Mycoplasma bovis OM OM kg TS OM DG DGkg α β g OM OM Trueperella pyogenes Tp Fusobacterium necrophorum Fn Mycoplasma bovis Mb Mycoplasma dispar Tp Fn Fn Tp Mb RS Fn Tp

8 NOSAI NOSAI Mycoplasma Mycoplasma Prominence Fischer nm Mycoplasma nm p Mycoplasma nm p Mycoplasma Fischer p Fischer Mycoplasma ROC Mycoplasma Fischer Mp Mp Mycoplasma bovis M.bovigenitalium M.bg M.bg M.bg A B M.bg A B M.bg A B SDS-page PCR M.bg M.bg M.bg BamH1 A M.bg M.bg B M.bg M.bg M.bg M.bg M.bg M.bg M.bg M.bg

9 Streptococcus uberis ube OS ube Str. dysgalactiae dysgenterococcus faecalis fcae. faecium fci A B C D E F F S16 rrna PCR ube cm 2 fci fca fci g ube B C D A EF ube fci nn gg IgG1 BHV BVDV BVDV2 RS BRSVBRV BCV IgG1mg ml mg ml IgG1 mg ml IgG1mg ml BHV1 BVDV2 BRSV IgG1

10 NOSAI FPT failure of passive transfer FPT FPT IgG mg ml t Mann-Whitney U x Fisher Kruskal-Wallis FPT RBC µl µl PLT µl µl BUN mg dl mg dl T-Chomg dl mg dl IgG mg ml mg ml Sial mg dl mg dl GOTU l U l LDHU l U l kg kg cm cm RBC P P FPT NOSAI l

11 NOSAI F14 Cr BCV BRV Cr l l l l l BCV BRV Cr EX ET l EX kg kget kg kg EX cmcmet cmcm EX kgkget kgkg ET EX EX ET CP CP

12 A IU dl B1 B1 cm NOSAI kg Levine φ cm φ cm MRI CT cm cm φ cm φ cm MRI CT

13 NOSAI BUN CRE P BUN CRE P BLV BLV caudal vena cava thrombosis : CVCT CVCT CVCT pulmonary thromboembolism : PTE CVCT PTE PTE PTE A G PTE

14 NOSAI X PIXE : Particle Induced X-ray Emission Ecoli Klebsiella pneumonia PIXE Mann Whitney U PIXE Al As Br Ca Cl Co Cr Cu Fe Ga Hg K Mg Mn Mo Na Nb Ni P Pb Rb S Se Si Sr Ti V Y Zn Fe µg g µg g p Zn µg g µg g p PIXE Fe Zn Zn NF-kB Fe Fe PIXE NOSAI NOSAI NOSAI NOSAI Charles River Laboratories Fe 3+ Hepc IL 6 IL-6 Fe Fe 3+ µg kg O-111 B4 LPS Fe 3+ Fe 2+ Dunnett-t Echerichia coli Klebsiella pneumonia Fe 3+ post-hoc Kruskal-Wallis ROC Fe 3+ p P Fe 3+ µg dl µg ml p ROC Fe 3+ µg dl p Se Sp Fe 3+ Fe 3+ Fe 3+

15 NOSAI NOSAI NOSAI NOSAI Charles River Laboratories A SAA SAA µg kg O-111 B4 LPS SAA LATIA Dunnett-t Echerichia coli Klebsiella pneumonia SAA post-hoc Kruskal-Wallis ROC p p SAA µg ml µg ml p ROC SAA µg ml p Se Sp SAA SAA SAA SAA NOSAI Ca VitD3 Ca Mg OR CI SPSS ver Ca OR VitD3 OR VitD3 OR Ca OR VitD3OR Ca Ca VitD3 VitD3

16 NOSAI Ca P kg BCS ml IV ml SC ml ml VS Ca ica ip Mg Ca IV mg dl SC mg dl VS mg dl ica Ca ip IV mg dl SC mg dll VS mg dl Mg IV Ca SC VS Ca ica IV SC VS SC VS K K K K T T K K K EP AB K meq l K meq l K meq l K K meq l K K P T T P T P K K K P mv T mv T P P T T P K T P K K T P rp K K K T P K

17 NOSAI VS VS Ket BHBA µmol l Mas Ute VS VS VS VS VS DIM p VS Ket Mas Ute Mas VS Ket Ute Mas VS DIM VS Mas DIM DIM VS VS VS VS NOSAI LCP DCPLCP LCP kg LCP LCP mm mm mmmm mm Lag LCP mg kg ml kg LCP LCP

18 NOSAI Velpeau Velpeau Velpeau Velpeau Velpeau Velpeau Velpeau kg mmhg mm

19 VF NOSAI kg kgkg mm V V cm BToV BToV RT-PCR HRT18aichi BToV spike Smembrane Mhemagglutinin-esterase HE nucleocapsid N BToV BToV BToV S M N S HE GM BToV BToV S HE BToV BToV GM BToV

20 NOSAI FNA cm WBC µl µl LDH U l TK U l kg TK

21 BLV BLV BLV ELISA PCR PCR PCR DNA DNA Wizard Genomic DNA Purification Kit, Promega DNA NestedPCR BLV BLV BLV BLV PCR KOD FX Neo Toyobo BLV PCR BLV NestedPCR KOD FX Neo PCR KOD FX Neo DNA BLV PCR rpcr rpcr ELISA rpcr rpcr rpcr rpcr rpcr rpcr

22 PCR rpcr rpcr L T L T L T L L T T rpcr L L T pg well pg well T pg well pg well rpcr rpcr rpcr rpcr rpcr NOSAI M M Ht M M

23 NOSAI M M M Theileria orientalis T.o PCR PCR T.o T.o T.o T.o M BSE BSE BSE BSE BSE BSE BSE L BSE BSE JP24 BSE BSE EX-ZR300 Move-tr 2D TMT441E BSE BSE BSE BSE

24 Clostridium perfringenscp CP A CFU g CP α C CP CP CP CP CP CP CP CP NOSAI D S D S G G G G D S

25 NOSAI X X X X NOSAI NSAIDs NSAIDs NSAIDs NSAIDs NSAIDs mg kg ivmg NSAIDs NSAIDs NSAIDs NSAIDs NSAIDs

26 NOSAI NOSAI JA Student t 1stDF1stDF 1stDF 1stDF E2 E2 E2 1stDF 1stDF 1stDF 1stDF 1stDF 1stDF 1stDF P vs 1stDF P vs 1stDF 1stDF 1stDF

27 NOSAI TAI Day0 mg PGimDay2µg Gn im Day9 PG im Day11 Gn im Day12 TAI TAI AI TAI TAI TAI AI TAI P ORCI AI P OR CI TAI NOSAI CIDR CIDR DF ; Dominant Follicle CIDR DF CIDR EBmg PGF2αmg CIDR DF EB mg AI CIDR DFmm CIDR mm DF EB AI second EB χ DF DFmmmm DF mm mm DF DF DFmmmm DF mm mm DF mm DF mm pp second EB DFmmp EB DF mm mm EB SE CIDR DF mmmmmmmmmm CIDR CIDR DF CIDR DFmm second EB EB second EB

28 NOSAI CIDR P4 g P4 CIDR P4 CIDR New n CIDR Used n CIDR-synch Day0 CIDR mg Day8 CIDR Day9mg Day10-11 AIDay0 1 7 P4Day0 7 CIDR New n CIDR Used n CIDR-synch Day7 PGF2αmg P4 SD ng ml Day0 1 7 New Used New Used New Used Used Day0 New Used CIDR P4ng ml CIDR P4 P4 Used P4 CIDR P4 NOSAI VWP

29 NOSAI n n vs p vs p vs Y cm cm WW PG 2W 3W 2W 3W 2W 3W SD 2W cm 3W cm 2W 2W 3W 2W cm 3W cm 2W 3W PG

30 l NOSAI A A R A NEFA mmol l BHBA mmol l BCS A NEFA vs mmol l p BHBA BCS NEFA r BHBA r A NEFA mmol l BHBA BCS BHBA NEFA

31 NOSAI T Student t χ p NOSAI NOSAI NOSAI F kgkg Fkg cm cm Fcm cmcm Fcm ml g g g Fg g g g F gg gg Fg gg g Fgggg Fg F F α CK IU l CK

32 ELISA Mann-Whitney U SPSS12.0J P Dairy Comp 305 Valley Agricultural software SPSS12.0J p BCS

33 NOSAI Carroll and Huntington NOSAI Carroll and Huntington p p nkg kg kg nkg kg kg nkg kg kg nkg kg kg

34 µg kgmg kgmg kg CRImg kg min mg kg K meq l ph K K meq l meq l CRI K K Na K mm cm cm cm PDS

35 NOSAI BCS limb perfusion cm X NOSAI X X MLBT-TIVA X

36 BTC Staphylococcus aureus Streptococcus equi Acinetobacter lwoffii Staphylococcus xylosus Staphylococcus vitulinus Enterobacter agglomerans Flavimonas oryzihabitans Staphylococcus sciuri JRA CFUµl PFU µl

37 C mm C MMC MMC mg ml ml C US US US US US US US Renosplenic Entrapment RE RE US US US US US

38 X-ray US US US US AAEP lameness grading MHz MHz X-ray US US US X-ray US US US X-ray X-ray US US US X ray US X HP http : //www. hba.or.jp/rep/onegai.pdf X Jackson Grade1 Grade2Grade3 Grade Grade Grade1 Grade2 Grade3 Grade2 Grade3 Grade3 Grade3 Grade3 Grade3 Jackson Grade1

39 CT CT CT kg CT CT CT Brightspeed Elite, GE Healthcere, Japan CT cm mmmm mm mm CT Tenoscopy Tenoscopy

40 Cre22 mg dl BUN31 mg dl Cre18 mg dl BUN42 mg dl K5.5 meq l PAS Luxol fast blue Concanavalin A wheat germ agglutinin Ricinus communis agglutinin 120 soybean agglutinin

41 Mycobacterium M. avium-intracellure complex

42 NOSAI Lawsonia intracellularis Li PCR BTHRbpm RR bpm SAA PCR Li BTHRbpm SAA PCR Li Li Li PCR JRA Lawsonia intracellularis Li EPE Li Li PPE EPE PPE Boehringer Ingelheim McCoy Li B3903 TCID50ml ml PCR Li PPE EPE EPE EPE EPE PCR EPE PCR PPE PPE PPE

43 nn n nn n n nnn n D L WI BL NLL D Ki-67 E-Cadherin IHC Ki-67 L D WI BL NL WI n nn n nl D WI BL NL D WI L WI IHC E- Cadherin Ki-67 Ki-67 L D WI BL NL WI Ki-67

44 S mm mm n mm n Ishii Ishii G1 G2 G3 G2 G3 G2 G3 G2 G1 G2 G3

45 G ml NOSAI

46 NOSAI GPS ATP ATP A B ATP x ml A B ml AB A BA:PB:P A B ml A B P A B P ATP logrlu A B A B A A ATP

47 vicugna pacos µg kg Sarcoptes scabiei var aucheniae µg kg O.P cm PRRS surface protective antigen type A SpaA SpaA SpaA

48 Salmonella sp. O4 io4 i - O4 i O4 i Salmonella TyphimuriumST O4 i ST A B C O4 i ST O4 i O4 i A B ST ST C O4 ia B A B O4 i O4 Salmonella O4 O4 ST O4 i O4 A B O4 i O4 i ST O4 i ST PCMV PCMV PCMV CPK PK-15 PCMV PCMV PCMV IgG PCMV

49 A PCV2 Salmonella O4 i PCV2 Salmonella O4 i

NOSAI NOSAI

F NOSAI NOSAI F F NOSAI NOSAI F NOSAI NOSAI NOSAI NOSAI NOSAI NOSAI Computed Radiography CR X Computed Radiography System FCR PRIMA T X PORTA 380HF GT GC GS RNRTREDRN DRTDRE IP GS RE IP CR NOSAI HH mm