NOSAI NOSAI

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1 C C NOSAI NOSAI NOSAI NOSAI NOSAI C C NOSAI NOSAI NOSAI NOSAI

2 NOSAI NOSAI

3 Computed Radiography CR X NOSAI NOSAI CR NOAI DICOM OSIRIX S S S S S S X IP S S S S X T4 Vertebral Heart Size VHS VHS VHS n WCS n n nosirix MD T4VHS T T n WCS WCS VHS T4 vv VHS T4 v v ROC p AUC Se Sp p AUC Se Sp VHS v v v VHS

4 Ambu ascope 3 Ambu ascope 3 Ambu ascope 3 mm mm Ambu ascope 3 mm mm Lux Lux Ambu ascope 3 Ambu ascope 3 kg Pm Coliform Pseudomonas Mh AL α-gl po 2 mmhg O2SAT A-aDO2 po2 O2SAT A-aDO2 po2 O2SAT A-aDO2

5 NOSAI K QRS T T K K K K K K AB K K meq l K p T μv p T K K T rp T K NOSAI Ca P kg BCS kg BCSPO2 PO3GCml Ca ica ip PV3 GC ml PO3 Ca mg dl PO2 ica ip PO2 PO3 Ca mg dl ica ip PV3 Ca ica ip

6 NOSAI Arkray L1L2 L3L4L3 Kruskal-Wallis Mann-Whitney U ROC L1 L2L3L4 L4 mmol l P mmol l mmol l P ROC mmol l L4 NOSAI ph n n n S ph HORIBA ph Tukey- HSD Kruskal-Wallis ph p ph p S ph ph ph ph

7 NOSAI NOSAI ml g kcal ml kg n n ph BE BHBArPV Dunnet-t Student-t rpv ph BE g dl g dl BHBA mm mm NOSAI AR AR ARD ml kg AR n ARD nbase Post hrs β BHBA rpv BE ml kg AR n ARD n Post rpv AR ARD BE AR ARD Post BE Base AR BHBA ARD Post BHBA Base ARD AR AR ARD

8 WCS WCS WCS kg g Fischer ml ml ml ml μl g dl mg dl nm ml WCS NOSAI mg kg immg iv mg kg im β mg kg im g iv BID X X β

9 NOSAI kg mg kg cm cm cm ml cm kg cm cm NOSAI A G Micrococcus luteus cm

10 CF CF CF CF NOSAI BUN Ca BUN mg dl Ca BUN Ca BUN BUN

11 kg HE μm μm H DG Daily gainh DG DG t

12 P3 P3 P3 P3 P3 P3 P3 P3 Peason P3 MHz P3 H0H1 H2H3 P3 S1 S2 S3 P3 P3 P3 χ 2 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 P3 DM P3 DM P3 DM DM MHz H1 H2H3 S1 S2 S3 P3 H1 P3 P3 χ 2 Fisher H1 S1 P3 H1 P3 H2 H1 DM P3 P3 P3

13 OR CI SPSS.ver18 SPSS Inc ORCI ORCI NOSAI CR CT CR CT Lal Lal ml ml Lal

14 NOSAI PT PT PT DTI n PT n Cal Lal Lal Lal Cal Lal Lal-Cal DTI PT Lal Lal-Cal cm cm PT Lal cm cm cm Lal DTI Lal-Cal Lal PT Lal -Cal Lal Lal -Cal Lal PT DTI Lal-Cal Lal PT DTI CCFT CCFT CCFT X Image J Mann-Whitney U CCFT kg CCFT X CCFT CCFT X

15 NOSAI X X cm cm X TPC K TPC kg kg kg K DCP K K TPC K DCP K TPC

16 kg mm DCP mm mm mm kg mm LCP LCP kg LCP cm LCP mm LCP GUmg kg LCP LCP

17 NOSAI X X X X X L M S ml ml Σ Σ L M S ml ml P L ml ml

18 TMR AjiPro Lg BHBA NEFA Dairy Plan C21 GEA SPSS 12.0J SPSS IncBHBA NEFA Mann-Whitney U NEFA μeq l μeq l PBHBA μmol l μmol l P P Dairy Comp 305 Valley Agricultural Software JMP 11 SAS P PG PG vs P P PG PG

19 NOSAI HH H Fisher Student t H H vs H :P H H vs H P NOSAI Dropped Udder : DU DU DU DU A AB DU index DUIDUIB A DUI DUI DUI DUI DUI SD paired t-test DUI r p n DUI p p DUI DU DUI DU DU

20 FPT FPT Brix TP Brix H HH TP Brix IgG TP Brix Brix IgG Brix Brix Brix Brix FPT CECDED Kenney B CDED CE ml CDED CDED CE CDED CE

21 Renaudin mm Renaudin Murase N F M N N N NNF M N

22 EEL EEL NOSAI JRA J.Equine Vet Sci P E NOSAI SD P E mgmg ST g g P E P ng ml P ng ml CTUPmm

23 kg Head-and-Tail Rope system Perfect Excellent A Good Not good B Fair Poor C A B C C α Sling Recovery LI SIμg kg μg kg mg kg ml kg mmhg LI g dl SI g dl LI SI HES Ca PaO2 mmhg Perfect Excellent Good Not Good Fair Poor Sling Recovery

24 Epiglottic Entrapment EE ADAFDDSP 8SEC ACG GPT VCC EE EE GPT SEC ACG EE NOSAI X X X X

25 mm mm X Transphyseal bridging breakover phase X SW SS SW SS SWSS SW SS SS SW SS

26 CXDI-801C Wireless X PORTA 380HF 80kV0.6mAs 80kV1.6mAs TOREK TLD msvmsv msvmsv msv msv msv msv msv msvmsv msv msv msv msv msv msv msvmsv msv msv NOSAI EPE Lawsonia intracellularis EPE NOSAI EPE EPE EPE EPE

27 cm cm cm kg

28 HGF GH HGFμg GH HGF GH HE HGF HE HGF HGF HGF HGF GH Hepatocyte Growth Factor HGF HGF HGF mm HGF mm HGF n n HGF S-100 HGF HGF

29 PCR rpcr rpcr rpcr A A B A A A S PCRrPCR rpcr A Y B T rpcr A Y rpcr B T rpcr Y T A rpcr

30 JD PCR PCR JD DNA JD DNA pg g JD JD DNA pg g JD JD DNA pg g JD JD NOSAI BDV RNA BDV BDV-N Western blot RNA BDV N RT-PCR BDV RNA BDV BDV

31 NOSAI BDBDV A, B C BDV Relative Risk : RR A B C BDV-N Western Blot BL A B C BLV PCR R RR BDV A B C BDV RR A B C RR CI BLV B BLV RR CI BDV BDV BLV BDV NOSAI OS S.uberis U OS U OS OS NK U S.dysgalactiae DEnterococcus E OS O OS U D E O Kruskal-wallis Fisher Kaplan-meier Log-rank U D E O E U vs D vs O U vs D vs O U vs D vs O U vs D vs O U U O D U D O U O U OS

32 NOSAI E.coli EC Klebsiella spp Kleb EC Kleb EC Kleb Log-Rank EC Kleb EC 63 Kleb45EC Kleb p EC Kleb p EC Kleb pec Kleb Kleb EC Kleb Kleb EC Kleb Kleb NOSAI E.coli Klebsiella spp n GNR β GNR E.coli68 Klebsiella spp 22 Enterobacter spp 5 Citrobacter spp 3 E.coli Klebsiella sppenterobacter Citrobacter sppabpc CEZ E.coli ABPC vs p E.coli Klebsiella Enterobacter Citrobacter CEZ Enterobacter Citrobacter E.coli K.pneumoniae E.coli Klebsiella Enterobacter Citrobacter CEZ Klebsiella Enterobacter Citrobacter

33 NOSAI HBS Clostridium perfringens CP A CPA α CPA α CPA α CPA α CPA α H H CPA α HBS NOSAI TSV-2 IBRV PI-3V γ IFN-γ TSV-2 H HH TSV-2 LOT102 H IBRV BVDV1 BVDV2 BRSV PI3V ADV Mh Pm Hs TSV-2 IBRV PI-3V BRSV TSV-2 BRSV TSV-2 BRSV RS TSV-2 IFN-γ BRSV

34 TSV-2 Zoetis IBR PI-3 n n Dairy Calf Tape The Coburn Company Log-rank Mann-Whitney Prism ver 6 GraphPad Software p NOSAI A RS

35 NOSAI BVDV LK BVDV1 BVDV2 RS Zoetis n n n p

36 NOSAI NOSAI NOSAI Sarcoptes scabiei Chorioptes spp. KOH Chorioptes C.texanus C.bovis C. texanus C.bovis Bovicola bovis Sarcoptes scabiei C.texanus C.texanus C.texanus C.bovis Chorioptes Sarcoptes scabiei Mt Mt Mt Mt Mt Mt Mt Mt B1 DONT-2 A ELISA RIDAScreen R-BiopharmAG Mt Mt Mt Mt Mt Mt Mt Mt Mt Mt Mt Mt Mt Mt M Mt Mt

37 NOSAI NOSAI mg μl OPG OPG IC BIO K155 BioX Diagnostics OPG OPG OPG OPG IC OPG OPG IC OPG IC OPG OPG OPG NOSAI BLV B Fine needle aspiration : FNA BLV FNA μl FNA B LDH U l U l φ cm cm φ cm B BLA-36 FNA

38 NOSAI EBL BLV BLV BLV EBL BLV A B A B EBL BLV A B BLV EBL EC BLV BLV BLV BLV BLV BLV A B BLV A B BLV A B A B A BLV B NOSAI BLV BLV BLV BLV FS A FS ELISA BLV nested PCR BLV BLV BLV PCR BLV BLV FS mmmm BLV A BLV FS BLV BLV FS BLV BLV BLV FS BLV A BLV FS BLV BLV

39 JA NOSAI BLV BLV BLV BLV BLV F1 BLV PHA PCR BLV H BLV HH BLV H HH F1 BLV BLV BLV BLV BLV BVDV PI PI PI PI PI RT-PCR BVDV1 BVDV2 PI PI PI PI BVDV1 BVDV2 PI PI PI PI BVDV1 PI BVDV2

40 BVDVPI PI PI S PI S1 PI S2 S5 N N BVD PI N1 N5 PI BVDV RT-PCR PI BVDV E2 S1 S2 1b S3 S5 1c N1 N4 1b N5 N1 N4 N1 S S1 S2N1 N S1 S2 S1 S2 N1 N N1 S N N2 N4 PI 1b 1c 1b N1 N BVDV BVDV BVDV BVDV H H H H H H HH H H H HHH HH H Vilcek 1b c a 1a H H1a 1c 1a 1c 1b 2a 1b H1a 2a 1a 1b 2a H 1a 1b

41 BSE ELISA BSE BSE ELISA BSE BSE BSE Mycoplasma bovis M.bovis kg X M.bovis μl Al g dl αgl g dl βgl g dl γgl g dl A G Al αgl

42 Mycoplasma spp. Mycoplasma spp.hayflic PCR Mycoplasma spp. Mycoplasma bovis M. bovis M. bovis Mycoplasma spp Mycoplasma spp M. bovis NOSAI OM Mycoplasma bovis M.bovis OI OI OI kg TS OI M.bovis M.bovis OI OM OI OM OI

43 NOSAI Mp PL Mp Mp Mp Mp Mp PL ppm Mp PL PL PL Mp Mp Mp Mp EHV1Streptococcus equi subsp. zooepidemicus Escherichia coli EHV1EHV1

44 NOSAI EHV-3 EHV-3 EHV -3 A EHV-3 EHV-3 mghr mg hr B EHV-3 C B EHV-3 EHV-3 A B C EHV-3 EHV-3 EHV-3 EHV-3 EHV-3 A B A C D E F G H A B SS-1 YS-1 DNA EHV-3 gg PCR EHV-3SS-1 A C D F EHV-3 DNA

45 JRA NOSAI 2ME IgM IgG 2ME DTT MAT 2ME-MAT 2ME DTT-MAT DTT 2ME M 2ME VBS DTT M DTT PBS MAT 2ME DTT MAT DTT MAT DTT MAT DTT MAT DTT MAT DTT MAT DTT MAT DTT MAT DTT MAT DTT 2ME DTT MAT DTT IgG DTT EHV EHV gg-elisa MDBK ORF ORF gg-elisa EHV EHV EHV EHVEHV EHV

46 NOSAI O4 isalmonella AberdeenAbSalmonella MeleagridisMeSalmonalla Heidelberg Ab Me Ab Me CLSI ABPC AMPC CEZOBFXERFX MARSM KMOTC ST Khan virulence genespvc invasion geneinva integron gene int multiplexpcr DNA Ab Me ABPC AMPC CEZ OBFX ERFX MAR GM OTC ST SM KM Ab Me inva spvc int Ab Me SM KM inva Me Ab NOSAI T O4 i S.Aberdeen O4 i is.aberdeen S.A i S.A P is.a P S.A S.A

47 HTBRB SRA HTB RB SRA HTB RB SRA S. Typhimurium HTB RB SRA S. Derby SRA S.Choleraesuis HTB S. Uganda RB SRA S. Abortusequi S. Dublin CFUg SRA O HTB RB SRA SRA RB RB SRA O HTB SRA SRA SRA OAT S.Typhimurium n DHL ndhl ndhl EEM BPW ndhl BPW BPW

48 g S.Typhimurium ST S.Infantis CFUml Na DHL ST TSA ml DHL NOSAI GS P P ml GS TDN P

49 NOSAI T OAT Salmonella Typhimurium S. Choleraesuis A, B, C A A A B C A B C A B C g RV ml DHL ES A Infantis, Derby, Caen B Derby C Derby, Livingston A p A

50 PED PED PEDV A B C IHC IHC PEDV PEDV PEDV S PEDV PEDV PEDV IHC IHC IHC PED PEDV PEDV A PEDV PEDV PEDV PEDV PEDV

51 X Lawsonia intracellularisli A D A B PCR T A D T Li PCR PCR B D T C B D T B A B C D Li Li Li HACCP HACCP JAS HACCP

52 E2 E2 Roelof et al P β P β P Horrell et al E E α NOSAI PGF2α Pursley PGF2α P4 A HONDA HS-101V mm n n PGF2αmg PGF2α mg PreDay P4 P4 PGF2α P4 ng ml Day P4 P4ng ml P4ng ml P4 Day Day P4ng ml n P4ng ml n P4ng ml Day P4ng ml Day P4ng ml Day P4ng ml P4ng ml P4ng ml PGF2α

53 P4 P4ET P4 ET P4 n ET ET A:n B: n P4n A B A:n B:n A:n B:n A:n B:n P4 ET P4 ET NOSAI A B A B A B P A B

54 ThCG T S S H H H H S D S-100b B H D S B S D B H T H S NOSAI cm cm α

NOSAI NOSAI

F NOSAI NOSAI F F NOSAI NOSAI F NOSAI NOSAI NOSAI NOSAI NOSAI NOSAI Computed Radiography CR X Computed Radiography System FCR PRIMA T X PORTA 380HF GT GC GS RNRTREDRN DRTDRE IP GS RE IP CR NOSAI HH mm