第２回脊髄再生研究促進市民セミナー
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1 URL Wise Young, Ph.D., Keck Center for Collaborative Neuroscience Rutgers University Aspire National Training Centre Aspire :Association for Spinal Injury, research, rehabilitation, and integration J. Aspire
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3 55 MBP-Cre/p35 transgenic mouse Pantelis Tsoulfas - 3 -
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8 ES Embryonic Stem Cell 10 Geron NIH reports Stem Cells H NIH Goeteborg University 19 CyThera, Inc. 9 Reliance Life Sciences 7 Monash University 6 Karolinska Institute 5 Wisconsin Alumni Research Foundation 5 BresaGen, Inc. 4 Technion-Israel Institute of Technology 4 National Center for Biological Sciences 3 University of California
9 Oct-3/4 SDIA stromal cell-derived inducing activity NIH
10 26 ( ) = Total market capitalization or valuation (estimated) Pittsburgh Tissue Engineering Initiative ndustry/pdf/industry.pdf Starzl
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14 10 11 IRB : Institutional Review Board
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46 ( ) [myelin: ( )] [myelin sheath: ] myelin myelin
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51 (2002 ) strategy neuroprotection brush up
52 A in vitro ES in vitro in vivo Neurosphere
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55 EGF FGF2 EGF+FGF EGF+FGF2 nestin EGF+FGF2 203 OLG OLG caspase 35 transgenic mouse MBP-Cre/CAG-loxP-neo-loxP-p35 mouse MASCIS p35-/- BBB scale SCANET TG 35 transgenic mouse OLG
56 MRI
57 Marin Oudega NYU 12 Schwann Schwann LA CA scaffold PLLA seeding 10 20mm Schwann scaffold seeding tube Schwann
58 mm 12 in vitro
59 19 Ramon y Cajal 1980 neural stem cell 3 Weiss neurosphere EGF epidermal growth factor FGF-2 fibroblast growth factor-2 neuroshere neurosphere neurosphere
60 Weiss 14 neurosphere nestin BrdU Weiss Weiss BDNF brain-derived neurotrophic factor Ghosh NT-3 neurotrophin-3 McKay PDNF platalet-derived neurotrophic factor CNTF ciliary neurotrophic factor T3 instructive LIF leukemia inhibitory factor BMP-2 bone morphogenic protein-2 CNTF LIF IL-6 interleukin-6 gp130 in vitro paracrine autocrine glial lineage mrna
61 BDNF CNTF BDNF CNTF CNTF CNTF survival assay prolifer-ation assay CNTF nestin CNTF BDNF 10 NT CNTF in vitro glinal lineage BDNF NT-3 pro-genitor in vitro CNTF BDNF 1 4 CNTF NGF nerve growth factor CNTF BDNF
62 GDNF NT-3 NT-4 in vitro CNTF 1 NT-3 BDNF CNTF BDNF CNTF CNTF permissive TNF IL-1 IL-1 IL TGF 4 TGF 7 14 Bregman
63 McDonald 1 Bregman
64 Profile Bregman 99 1 Vol.201 No What s new on neural stem cells and other stem cells?
65 pro-spective in vitro expand
66 ES inner cell mass ES ES ES ES PLP ES PA-6 Parkinson ES ES key enzyme tyrosine hydroxylase green fluorescence protein cdna TH-EGFP TH ES in vivo functional Parkinson ALS Huntington GABA Purkinje ES
67 ES ES SCID ES ES embryoid body HLA ES ES therapeutic cloning Advanced Cell Technology ES B ES ES
68 turn over ES 2 3 cell fusion
69 mesen-chymal stem cells MSC skin-derived precursors SKP MSC SKP MSC MSC ES
70 neural stem cell Parkinson neural stem cell
71 1992 Calgary Weiss neurosphere 1994 Albany Davis 1995 Salk Gage neurosphere neurosphere epidermal growth factor EGF fibroblast growth factor2 FGF2 neurosphere Neuro-sphere Nestin Musashi1 Nestin 1990 Musashi kDa RNA Musashi1 Musashi1 Notch Nestin Musashi1 Nestin Musashi1 in vitro neurosphere neurosphere Nestin Musashi1 embryonic neural stem cell Cambridge Svendsen Wisconsin Vescovi neurosphere 1 neurosphere leukemia inhibitory factor LIF Carpenter EGF FGF2 LIF LIF neurosphere
72 ex vivo in vivo Gage BrdU BrdU Cornell Goldman Musashi1 in vitro in vitro Pagano Louisville Roisen 18 neurosphere adult neural stem cell
73 neurosphere prospective pro-spective Nestin Musashi1 fluorescence-activated cell sorter(facs) prospective Goldman Musashi1 Nestin enhanced green fluorescent protein EGFP EGFP FACS FACS CD133 Uchida CD133 + CD34 CD45 neurosphere Klassen CD9 CD15 CD81 CD95 GD2 ganglioside major histocompatibility complex MHC class CD
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75 Rho small GT Pase 1911 Tell 1928 Cajal 1980 Aguayo Schwann Schwab IN
76 IN-1 Nogo Nogo myelin associated glycoprotein 1 slit ephrin semaphorin slit Robo ephrin Eph semaphorin plexin Robo Rho GTPase activating protei small
77 GTPase cdc42 EphA ephexin Rho Rho guanine nucleotide exchange factor plexin Rho Rac TNF Rho p75 Rho plexin p75 small GTPase small GTPase MAG in vitro Pho C3 Rho MAG MAG p75 MAG p75 MAG camp Rho
78 IN-1 PROGO Rho in vitro FRET
79 Wise Young M.D. Ph.D
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85 The Society for Neuroscience 1980 Society for Neuroscience SCI " " " SCI 1981 Society for Neuroscience 10 SCI Neurotrauma Society 1988 Neuro-trauma Society 500 the Jornal of Neurotrauma INTS (Internatio-nal Neurotorauma Society INTS 800 SCI
86 1960 IMSOP International Medical Society of Paraplegia 1980 ASIA American Spinal Injury Association AANS CNS CSRS Cervical Spine Research Society Japan Paraplegia Society Korean Neurotrauma Society 1979 the International Spinal Monitoring Society 1980 PVA (Paralyzed Veterans of America) SCS (Spinal Cord Society), the Help Them Walk Again Foundation PVA SCS Dr.Chuck Carson PCR DC The Help Them Walk Again 1979 by D.W. SCS littany Dr.Charles Carson 80 SCS
87 SCS SCS Trink Gardner 1970 Bermuda Conferences Walkman Award Walkman Award ISRT (International Spinal research Trust Fund) 40 Kent Waldrep Foundation Steven J. Camhi Fund 1980 Marc Buonocanti Foundation Miami Project SCI 1990 Miami Project Bunge Dick Mary SCI NSCIA (National Spinal CordInjury Association) 1980 SCI 1980 Daniel Heumann Fund Alan T. Brown Foundation Ameritec Foundation Tom and Beatrice Hollfelder CPA (Canadian Paraplegia Association), CSRO (Canadian Spinal Research Organization), Rick Hansen Man-in-Motion Foundation ASRT (Australasia Spinal Rsearch Trust) ISRT (International Spinal Reseach Trust)
88 1987 PVA NCSCIA (National Council for Spinal Cord Injury Association) 1993 Arthur Ullian NCSCIA NCSCIA SCI Arthur NCSCIA END (The National Coalition to End Neurological Disorders Dana Alliance END END Paralyzed Veterans of America DC 1981 PCR APA David Camhi Kent Waldrep APA NYU Medical Center 3 AP Dr. Andrew Blight NYU Impactor the now standard rat spinal cord injury Dr. John Gruner Admiral Dick M.D. Van Orden Kent Waldrep APA David Camhi Hank Stifel, Joe/Michelle Alioto APA APA Henry Stifel Foundation (Hank and Charlotte Stifel) Kent Waldrep NPF National Paralysis Foundation APA Aliotos National Paralysis Project APA APA (Martin Schwab, Eric Shooter, Carl Cotman, Rusty Gage, Ira Black, and others) APA
89 1994 Kent Waldrep APA NPF APA Miami Project 7 Alliance and Consortium APA APA Mitch Stoller APA Susan Howley, APA Consortium Charles Carson, Sam Maddox, Greg Winget, Ron Cohen, Benjamin Reeve, Shawn Friedkin, Ray Wickson, Larry Johnson, Lisa Hudgins, Peter Banyard, Margaret Brown, Marilyn Spivak, Bob Yant, Mary Ann Liebert, Vivian Smith, Cheryl Chanaud, Lesley Hudson, Walter Schafer, George Zitnay, Vivian Beyda, Tim Hanlin, David Mahoney, Gayle Stevenson
90 Barbara Walters, Katie Couric, Diane Sawyers, Bill Utley, Christopher Reeve Murray Goldstein, Margaret Giannini, David Gray, Doug Walgren Spine Wire HP Sidall
91 Millan ATP NO pronociceptive NMDA NMDA Basbaum 10 P PPK C PKC Polgar PKC PKC
92 Vilim NPFF NPAF NPSF 1999 Colburn Ignatowski TNFá
93 P GABA Dickinson Fleetwood-Walker VIP PACAP Fisher Hagen Huang Simpson c-fos Munglani 20 Laffey Seaman Cleaveland
94 Kontinen Wise Wire
95 2000 Dr. Pantelis Tsoulfas The Miami Project to Cure Paralysis, a Center of Excellence at the University of Miami School of Medicine 1985 Dr.Bath Green CNS
96 pro-genitors ES totipotent multi-potent multi-potent neural progenitor cells Pantelis Tsoulfas M.D
97 -axon myyelin 1980 immortalized cell lines
98 the Salk Institute Fred Gage Ph. D. Scott Whittemore Ph.D. Louisville vs. RN33B ( ) RN33B setrules Evan Snyder M.D
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