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ゆゆこてらわ
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56 2 2010 3 19 50 A 2007 10 45 60 2007 12 30 2008 1 31 A 2 1 1 2 3 4 5 6 2 PCV2 7 PCV2 in situ 8 9 PCV2 [1, 2] PCV2 PCVAD 1. Jensen, T. K., Vigre, H., Svensmark, B. and Bille Hansen, V. 2006.

3 A A PCV2 7 PCV2 in situ 8 9 PCV2 [1, 2] PCV2 PCVAD 1. Jensen, T. K., Vigre, H., Svensmark, B. and Bille Hansen, V Distinction between porcine circovirus type 2 enteritis and porcine proliferative enteropathy caused by Lawsonia intracellularis. J. Comp. Pathol. 135 : Komarek, V., Maderner, A., Spergser, J. and Weissenbock, H Infections with weakly haemolytic Brachyspira species in pigs with miscellaneous chronic diseases. Vet. Microbiol. 134:

4 20 9 1 X 1 2 3, 4 Palisading granuloma 1. Gross T. L. et al. 2005.

4 X 1 2 3, 4 Palisading granuloma 1. Gross T. L. et al pp In:Skin Diseases of the Dog and Cat, 2nd ed. Blackwell. 2. Lee M. W. et al Clin. Exp. Dermatol. 24:

5 VLDL very low density lipoprotein, LDL low density lipoprotein HDL high density lipoprotein LDL HDL HDL VLDL LDL

6 DNA 1983 Palmiter 8% 0.7% 0.4% ES embryonic stem ES ES FACS fluorescence activated cell sorting GFP green fluorescence protein FACS

56 2 2010 7 23 1% 2002 1,3GT 4 5 2 1,3GT 3 ES ES 1/10 6

7 % ,3GT ,3GT 3 ES ES 1/10 6 1/ cm 10 6 ES GFP 6 2 hdaf human decay accelerating factor Gal EndoGalC endo galactosidase FACS hdaf FACS Gal Gal 1,3GT 1,3galactosyltransferase 2

8 EndoGalC 95% Gal 100% 100% Gal 1,3GT National swine resource and research center Pursel, V. G., Bolt, D. J., Miller, K. F., Pinkert, C. A., Hammer, R. E., Palmiter, R. D. and Brinster, R. L Expression and performance in transgenic pigs. J. Reprod. Fert. Suppl. 40: Onishi, A., Iwamoto, M., Akita, T., Mikawa, S., Takeda, K., Awata, T., Hanada, H. and Perry, A. C. F Pig cloning by microinjection of fetal fibroblast nuclei. Science 289: Polejaeva, I. A., Chen, S. H., Vaught, T. D., Page, R. L., Mullins, J., Ball, S., Dai, Y., Boone, J., Walker, S., Ayares, D. L., Colman, A. and Campbell, K. H. S Cloning pigs produced by nuclear transfer from adult somatic cells. Nature 407: Lai, L., Kolber Simonds, D., Park, K. W., Cheong, H. T., Greenstein, J. L., Im, G. S., Samuel, M., Bonk, A., Rieke, A., Day, B. N., Murphy, C. N., Carter, D. B., Hawley, R. J. and Prather, R. S Production of 1,3 galactosyltransferase knock out pigs by nuclear transfer cloning. Science 295: Dai, Y., Vaught, T. D., Boone, J., Chen. S. H., Phelps, C. J., Ball, S., Monahan, J. A., Jobst, P. M., McCreath, K. J., Lamborn, A. E., Cowell Lucero, J. L., Wells, K. D., Colman, A., Polejaeva, I. A. and Ayares, D. L Targeted disruption of the 1,3 galactosyltransferase gene in cloned pigs. Nat. Biotechnol. 20: Watanabe, S., Iwamoto, M., Suzuki, S., Fuchimoto, D., Honma, D., Nagai, T., Hashimoto, M., Yazaki, S., Sato M. and Onishi, A A novel method for the production of transgenic cloned pigs :electroporation mediated gene transfer to non cultured cells and subsequent selection with puromycin. Biol. Reprod. 72: Yazaki, S., Iwamoto, M., Onishi. A., Miwa, Y., Suzuki, S., Fuchimoto, D., Sembon, S., Furusawa, T., Hashimoto, M., Oishi, T., Liu, D., Nagasaka, T., Kuzuya, T., Maruyama, S., Ogawa, H., Kadomatsu, K., Uchida, K., Nakao, A. and Kobayashi, T Successful cross breeding of cloned pigs expressing endo galactosidase C and human decay accelerating factor. Xenotransplantation. 16: Web site:

56 2 2010 9 25 LDH 2 A B 4 4 A4, A3B1, A2B2, A1B2, B4 LDH 1 LDH LDH A DNA 2 LDH B e crystalline 3 (A) LDH A LDH A GeneBank Accession No.

9 LDH 2 A B 4 4 A4, A3B1, A2B2, A1B2, B4 LDH 1 LDH LDH A DNA 2 LDH B e crystalline 3 (A) LDH A LDH A GeneBank Accession No. AB3818 SP 1 AP 2 CREB TATA BOX G C mrna 5 RACE cdna 4 clone RNA clone 3, 4 G LDH genome CTCACCCACCCCGCTACGACCGCAGCCGCGCTAGAAGGGGAAGACGCCGG(gtag---intron---tttcag)CAGTACACC CE5 clone1 GGGGGGGGGCCCGCTACGACCGCAGCCGCGCTAGAAGGGGAAGACGCCGG CAGTACACCATGTCTCTCAAGGA CE5 clone2 GGGGGGGGGCCCGCTACGACCGCAGCCGCGCTAGAAGGGGAAGACGCCGG TACACCATGTCTCTCAAGGA CE5'clone3 GGGGGGGGGGGGGCTACGACCGCAGCCGCGCTAGAAGGGGAAGACGCCGG TACACCATGTCTCTCAAGGA CE5'clone4 GGGGGGGGGGGGGCTACGACCGCAGCCGCGCTAGAAGGGGAAGACGCCGG CAGTACACCATGTCTCTCAAGGA (C) (B) A RNA 5 RACE 4 5 RACE C G 2 3 B deletion plasmid deletion plasmid plasmid ND C B plasmid EGFP MW; C1; pegfp C1, NC;

10 10 26 G G cdna 5 RACE A 5 RACE ORF LDH ORF LDH ORF ATG 1,200 bp 1B deletion EGFP 1, B HeLa RNA EGFP 1B plasmid BBs plasmid EBs RNA bp ATG A +1 plasmid S 48 S EGFP 48 1C plasmid BBs plasmid EBs RNA EGFP bp plasmid S EGFP plasmid S RNA plasmid S HeLa RNA 5 RACE 2A 3 S1, 42 S2 52 S3 S in vivo 48 embryo RNA oligodt 3 RACE 5 RACE LFH START 1 5,LDH 42 2B 1 ST 3 oligodt RNA polya 1 RNA polymerase II A LDH A genome 5 RACE HeLa plasmid S RNA 5 RACE 1 5 RACE A 5 RACE genome S2,3 genome S3 plasmid S HeLa plasmid S LDH START LDH 42 B PCR 5 B 48 embryo RNA oligo dt adapter (A) oligo dt adapter M4 PCR ST 42 PCR 5 (A) LDH START LDH 42

56 2 2010 11 27 In vitro HeLa plasmid S amanitin PCR 1, 2 SV40 major late Neo PCR amanitin pol II 7, 8 RNase plasmid S HeLa Cell in

polymerase II RNA polymerase I III polya polya RNA LDH ORF 100 base 4 LDH3 14 48 embryo RNA RT PCR LDH A LDH A embryo RNA LDH A ORF

11 In vitro HeLa plasmid S amanitin PCR 1, 2 SV40 major late Neo PCR amanitin pol II 7, 8 RNase plasmid S HeLa Cell in vitro in vitro 3 RNA oligo dt M4 LDH 42 PCR polya RNA 3, 4 EGFP ORF 5 RT PCR polya RNA 5, 6 RNA 2 polya RNA 20 g/ml mrna RNA polymerase II RNA polymerase I III polya polya RNA LDH ORF 100 base 4 LDH embryo RNA RT PCR LDH A LDH A embryo RNA LDH A ORF +890 LDH3 7 LDH LDH START LDH 42 PCR 2 polya RNA embryo 4 PCR polya RNA RNA GeneBank Accession No. AJ LDH A 1,200 bp LDH A 5 LDH A 2

12 12 28 ORF 2 mrna RNA 2 polya RNA polymerase II poly A RNA polymerase I III LDH A LDH A bp IE LDH A RNA J W, Kim Trends Biochem. Sci. 30: Cattaneo, A Exp. Cell. Res. 161: Wiljan H., Proc. Natl. Acad. Sci. USA. 85: URL lin.go.jplin.gr.jp 22 3 lin.go.jp 22 4 lin.go.jp

Letter585.pdf

Letter585.pdf http://nibs.lin.gr.jp/ 2 18 1985 29 1982 2013 3 12 7800 14.4 1997 35 44 2013 7 25 44 2020 3 6 60 2 2014 3 19 8 kg 1 Metastrongylus 2 3 4 1.. 2013.. 66 4 243 247. 2.. 2012.. 65 6 429 435. 4 20 2000 [1 2]