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1 AxioVision

2 A New Way of Thinking AxioVision 100 AxioVision Shuttle & Find SEM-LM Linkam Stage Control Linkam Fluorescence Lite 2 HDR Widefield Multichannel Unmixing TMA TMA Fast Acquisition Imaging Plus QuantiFISH Panorama & ApoTome ApoTome 2Ch MosaiX 3D 2D Z- 2D 2D 32Ch Inside4D 2

3 AxioVision Easy start AxioVision Easy to use AxioVision Easy ZVI ZVI Easy economy AxioVision Calotte Grinding Measurement TIC 3D 3D AutoMeasure Plus AutoMeasure Coal Random Reflectance Comparative Diagrams Layer Thickness Measurement Particle Analyzer NMI Graphite AxioVision Grains VBA Visual Basic Multiphase Asset Archive Commander 3

4 AxioVision AxioCam TWAIN 4

5 Basic Program AxioVision Microsoft Excel AxioVision 5

6 AxioVision Z AxioVision AxioCam Z- 3 Panorama 1 1 MosaiX FITCPlan-Neofluar 10x AxioCam MRm Panorama

7 Image Acquisition * MosaiX * 96 HDR HDR High Dynamic Range HDR 32 6FISH Dr. Michael Speicher, Medical University of Graz, Austria Z- Z- Z- ApoTome Z- 7

8 Z MosaiX Z-ApoTome VivaTome 8~256 Rhodamine Fast Acquisition Z- Cell Observer HS AxioCam HRm / XL S1 / 2 Fast Acquisition AxioCam 2 2 8

9 Image Acquisition AxioCam AxioVision LE Institute of Experimental Surgery, Groshadern Clinic, Germany Fluorescence Lite AxioVision LE 6 Shuttle & Find ROI Linkam Stage Control LinkamZVI 9

10 1 Imaging Plus Imaging Plus Elastic Registration 2 4 m TetraSpeck Microspheres, Invitrogen 10

11 Image Processing 3D/4DAxioVision 2D / 3D 3D 3D PSF 3D ApoTome ApoTome 3D ApoTome 2D 2D Z-2 Z-2 xz xy Nearest Neighbour 3 Regularized Inverse Filter 4 Fast Iterative 5 Constrained Iterative 6ApoTome PC12 Z- DAPI Alexa-488 Alexa-568 Campbell Cold Spring Harbor, USA Inside4D 3 Z- ApoTome Z- 3D 1 RAW Dr. Brigit Kraus, University of Regensburg, Germany 2 RAW Dr. Brigit Kraus, University of Regensburg, Germany Widefield Multichannel Unmixing DAPI/CFP CFP/GFP 8 ACE 2 Unmixing GFP CFP CFP Multichannel Unmixing GFP CFP GFP 11

12 AxioVision csv Xanthoria parietina 90 AutoMeasure Microsoft Excel 12

13 Image Analysis AutoMeasure Plus Region Growing Microsoft Excel D 3D 3D 3D 4 13

14 1Ch2Ch ph 1Ch Fluo-42Ch Fura-2 Indo Fast Acquisition Colibri Fura-2 Dr. Roberto Levi and Dr. Randi Silver, Weill-Cornell University, New York, USA Mouse hippocampus cultures in Different Interference Contrast axonal transport processes analyzed using the Tracking module Sample Prof. Okabe, Tokyo Medical University, Dept. f. Cell Biology, Japan 14

15 Image Analysis TMA TMA QuantiFISH FISH DAPI Z- 2 phaxiovision 2 * * 15

16 Z- Z-3D TIC TIC 0 Calotte Grinding Measurement DIN V ENV 1071 Calotte Grinding 1 DIN V ENV 1071 Calotte Grinding 16

17 Image Analysis Particle Analyzer ISO 4406 ISO VDA m Axio Imager 2 25 m SteREO Discovery NMI Non-Metallic-Inclusions MosaiX EN DIN AxioVision NMI EN ASTM E ISO DIN JIS G

18 Grains 3 ISO ASTM E ASTM E ISO ASTM E ASTM E SEP ASTM Plate Plate Plate Plate BS4990 Multiphase 32 Layer Thickness Measurement Chord Length [µm] No of Chords Description Distance [µm] Minimum Maximum Mean Std.Dev Range

19 Image Analysis Comparative Diagrams 8 Graphite ISO ASTM EN 3 Coal Random Reflectance Axio Imager EC Plan-Neofluar 50x/1.00 Oil AxioCam MRm AxioVision Coal Random Reflectance 19

20 Archiving AxioVision Asset Archive Configulation AxioVision AxioVision 2AxioVision Commander 1 VBA AxioVision VBA Visual Basic for ApplicationAxioVision 20

21 bmp tif jpg gif tga png j2k jp2 mac msp ras pct eps wmf psd img cmp zvi lsm czi vgi rek raw avi zvhi bmp jpg tif tga png psd avi lsm mov j2k jp2 pcx wmf pct AxioCam TWAIN 8bit 16bit 3200K / 3200 K AxioCam HR) 3 TTL 3 / / / / Z- Z- xyz 3 / Z- 3 /

22 12 / / AxioVision AxioVision PC Microscope Configuration MTB2004 Axioplan 2 Axioplan 2 imaging Axiovert 100M Axiovert 200M Axioskop 2 mot Axio Observer. Z1 / D1 Axio Examiner Axio Imager.Z2 / M2 / Z1 / M1 Axio Imager. A2 / A1 Axio Imager. D1 / D2 SteREO Discovery. V20 / V12 / V8 SteREO Lumar. V12 Stemi 2000C / CS 32 Z- Z- Z- 3DApoTome Z- Z- Z- Z- 22

23 MosaiX 1 Fast Acquisition - ZVI / / Panorama / 23

24 ZVI Indo-1 FRET Fast Acquisition 2 HDR HDR HDR HDR HDR HDR HDR HDR HDR HDR Shuttle & Find ROI 100ROI 25 m Fluorescence Lite AxioVision LE 6 1 PDF Linkam Stage Control Linkam AxioVision avi mov 3D Z- PSF Nearest Neighbor Regularized Inverse Filter Fast Iterative Constrained Iterative ZVIPSF Z- Z- 3D Z- 3D non-regularized) Z- 3D ROI GCV Clip / AutoLinear / MatchInput) 24

25 2D 2D Z- Regularized Inverse Filter Fast Iterative Constrained Iterative 12D GCV GCV Z- Inside 4D Z-8 avi QuickTime 3 Widefield Multichannel Unmixing ACE Z Imaging Plus / 2 / / / 25

26 Watersheds / 2 / / / / RGB HLS RGB RGB RGB / / / / / / X Y X Y AutoMeasure 26

27 AND OR Excel CSV ZVI ON OFF AutoMeasure Plus Canny Marr / / / AND / OR / XOR / NOT / / / / / 1skeleton AND OR 3D 3D 3D 3D 3D 3D 2DZ- ROI ExcelCSV 27

28 ROI 4 Fura-2 GFP Fluo-4 Indo-1FRET 100 ZVI 1ChZVIFRET 2 ROI QuantiFISH Z- EDFFISH Excel CSV TMA MosaiX MosaiX AxioVision 2D Particle Analyzer PC VDA19 ISO NMI PC 1 EN DIN ASTM E 45 JIS G 0555 ISO

29 Grains ASTM-Plate Plate Plate Plate SEP BS 4990 DIN EN 623 ASTM E 112 ASTM E 1382 / DIN EN 623 ASTM E 112 ASTM E 1382 Multiphase Graphite EN ISO 945 SinterCast Frake Iron Gray Iron SAE J 1887 EN ISO 945 EN ISO 945 Comparative Diagrams Layer Thickness Measurement 29

30 Calotte Grinding Measurement Calotte Grinding DIN V ENV 1071 TIC TIC C-DIC 3D Z- Height Map 3D EN ISO 4287 Flooding Primary Waiviness Roughness Coal Random Reflectance PC 6 V Asset Archive SQL Commander VBA Visual Basic Visual Basic Editor AxioVisionVBA 30

31 Geometric AcpX, AcpY X Y Area AreaConvex, AreaFilled / CenterX, CenterY X Y EllipseMajor, EllipseMinor EllipseAngle X Perimeter Perimeter Convex Perimeter Filled Perimeter Crofton, Perimeter Crofton Filled / Perimeter X, Perimeter Y XY Perimeter X filled, Perimeter Y filled XY Perimeter XY, Perimeter XY filled Bound Top, Bound Bottom, Bound Left, Bound Right X YX Y Bound Width, Bound Height X Y Area Frame Feret Minimum, Feret Maximum Feret Minimum Angle, Feret Maximum Angle X FeretRatio Diameter, Radius Form circle Fiberlength ID Region ID Distance measurement 2 Angle measurement Densitometric Mean Standard deviation Minimum, Maximum Sum, Sum Square Geometric Area sum Area percent Number of regions Densitometric Perimeter sum Densitometric mean value Densitometric standard deviation Densitometric Minimum, Densitomertic Maximum 31

32 Printed in Japan P11 MIC 011 PR SK-01/ TM

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