Breakthrough technology that sets the new standard for flow cytometry 2 BD FACSCanto II

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5 years ago
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1 BD FACSCanto TM II Innovation with Impact

2 Breakthrough technology that sets the new standard for flow cytometry 2 BD FACSCanto II

3 1973 BD 30 BD FACS 14,000 BD FACSCanto II BD FACSCanto II 3

4 BD FACSCanto II 30 8 Flexibility 6 8 color * 8 Pacific Blue AmCyan Alexa405 CFP YFP FRET Qdot Quality * 8 color 2 2 BD FACSCanto II Time Delay PE- Cy5 APC PE-Cy7 APC-Cy7 Cy7 2 4 BD FACSCanto II

5 Innovation designed to driver quality, productivity, and flexibility Quality 10,000/ Productivity BD FACSCanto II 5

6 Advanced Applications 488nm Green FITC Alexa Fluor 488 Yellow PE Red PE-Cy5 PerCP Far Red PerCP-Cy5.5 Infra Red PE-Cy7 633nm HeNe Red APC Alexa Fluor 647 Far Red Alexa Fluor 700 Infra Red APC-Cy7 405nm Blue Pacific Blue Alexa Fluor 405 Cyan AmCyan 4 C57BL/6J 7 7-amino-actionomycine Far Red T B NK Mouse T, B, NK Cell Analysis C57BL/6J 7-aminoactionomycine T Pacific Blue-A APC-A CD4 CD25 FITC-A PE-A Mouse Regulatory T Cell Analysis 6 BD FACSCanto II

7 Jurkat Hoechst Pacific Blue-A Pacific Blue-A Cell Cycle BD Cytometric Bead Array (CBA) kit BD CBA Flex Set cytokine 12-plex acquired with the BD HTS loader BD FACSCanto II 7

8 BD FACSDiva TM BD FACSDiva TM CS&T BD FACS Canto TM BD FACSCanto TM II BD TM LSR II BD FACSAria TM FACS FACS B SDEN GMP BD FACSDiva PMT Qr Br 3 PMT 8 BD FACSCanto II

9 PMT delay PMT laser delay base line base line file PMT PMT FACS Cytometer Performance Report Levey-Jennings BD FACSDiva PDF snapto plot BD FACSDiva 1000 FSC BD FACSCanto II 9

BD FACSDiva TM CST CST Diva Auto compensation FACS Diva soft CS&T Baseline 16 PMT Base line back ground Application Setting Optional CS&T Daily Performance Tracking 3 Cytometer Performance Auto

10 BD FACSDiva TM CST CST Diva Auto compensation FACS Diva soft CS&T Baseline 16 PMT Base line back ground Application Setting Optional CS&T Daily Performance Tracking 3 Cytometer Performance Auto compersation BD FACSDiva TM Plots: Dot, contour, density, histogram Templates: Experiment, Specimen (Panel), Analysis, and Plate Data Transforms: Linear, 4 and 5 decade log, biexponential Export Formats: Statistics CSV, XML Data FCS 2.0, FCS 3.0 Displays/Plots JPEG, XML Worksheets PDF Experiments ZIP, XML Data Displays: Area, height, width, time, and ratio Import Formats: Data FCS 2.0, FCS 3.0 Calculated Performance parameters: Gating Types: Detection efficiency (Qr), electronic noise, optical background (Br), laser delays, linearity, robust CVs and medians of setup reagent beads, area scaling factors Hierarchical gating including Snap-To, Autopolygon, Polygon, Rectangle, Snap-To Interval, Autointerval, Interval, Quadrant, and Tethered Performance Tracking Boundaries Operating Systems: Derived Gates: Invert, Intersect, Join, and Rest of Instruments Supported: Statistics: % Parent, % Grandparent, % Total, Median, Mean, Geometric Mean, Mode, Standard Deviation (SD), % Coefficient of Variation (CV), robust % CV (rcv), robust SD (rsd), Population Maximum, Population Minimum System Options Supported: BD FACSDiva is for For In Vitro Diagnostic (IVD) Use when used with IVD reagents and cytometers. Cytometer Setup and Tracking is For Research Use only. Not for use in diagnostic and therapeutic procedures. Standard deviation, minimum, maximum Windows XP (SP2) BD FACSCanto BD FACSCanto II BD FACSAria BD LSR II BD FACS Loader (for BD FACSCanto and BD FACSCanto II), HTS (for BD LSR II, BD FACSCanto, and BD FACSCanto II), BD AccuDrop (BD FACSAria only) 10 BD FACSCanto II

11 Automated sample acquisition BD FACSCanto II 2 High Throughput Sampler HT Carryover 1% BD FACS High Throughput Sampler BD FACS Loader 12 75mm BD Falcon TM BD FACS BD FACSCanto II 11

BD FACSCanto TM II FITC 100 MESF, PE 50 MESF 1 µm 0.5 µm 20 mw, 488 nm 17 mw, 633 nm HeNe 30 mw, 405 nm 9 65 µm N/A = 1.

12 BD FACSCanto TM II FITC 100 MESF, PE 50 MESF 1 µm 0.5 µm 20 mw, 488 nm 17 mw, 633 nm HeNe 30 mw, 405 nm 9 65 µm N/A = nmpmt nm nm nm nm nm 633 nmpmt nm nm nm 405 nm PMT nm nm 480/10 480/10 PMT 1.1 L/hr 1 ml/hr BD FACSCanto Clinical Low = 10 µl/min Medium = 60 µl/min High = 120 µl/min 10,000 /sec 50 µm , Threshold 91 cm 61 cm 64 cm 146 kg 168 kg 100/115/230 VAC 50/60 Hz 500 W 79 cm 61 cm 64 cm 51 kg % 07B1X R

MACS機器予算申請カタログ

MACS機器予算申請カタログ MACS Instruments MACSQuant automacs MultiMACS gentlemacs MACSmix TM MACSQuant 3 MACSQuant Analyzer 10 MACSQuant VYBMACSQuant X MACSQuant X Coming soon! 384 well plate Laser Channel Filter (nm) Dye or Parameter