ZEISS LSM 880 with Airyscan Revolutionize Your Confocal Imaging
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- いちえい しもね
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2 Confocal Airyscan Airyscan 1.7 LSM confocal volumeclsm S/N -Alexa 546 AP3 Alexa 488 DAPI HeLa S. Traikov, BIOTEC, TU Dresden, Germany Airyscan >> 2
3 Simpler. More Intelligent. More Integrated. Airyscan: CLSM x y z 1 CLSM Airyscan CLSM XY140 nm Z 400nm 488nm LSM 880 / LSM 880 GaAsPdescanned non-descanned NDD LSM fps LSM 880 Confocal Airyscan Alexa 488 Na,K-ATPase GAR-CF 633 Plan-APOCHROMAT20x/ m MIP GFP HP-1 HP-1 HP-1 P. Hemmerich, Leibniz-Institute for Age Research FLI, Jena, Germany Histone2B H2B,,mCherry end binding protein3 EB3 GFP Hela-Kyoto J. Ellenberg, EMBL, Heidelberg, Germany. 3
4 Your Insight into the Technology Behind It Resolution SNR 24.0% Z 24.7% Confocal CLSM LSM 880 XY 25.3% 15.1% 11.0% Speed Airyscan SNR CLSM 250 Airyscan S/N 10 µm Confocal Airyscan higher Resolution Airyscan higher SNR Airyscan higher Speed 4
5 Your Insight into the Technology Behind It Airyscan Airy3 PSF CLSM 1Airy S/N Airyscan ZEISS 32Ch Airy Airy S/N LSM Confocal Airyscan µm HEp2 GFP V. Döring, Leibniz-Institute for Age Research FLI, Jena, Germany Airy 5.Airyscan 5
6 Your Insight into the Technology Behind It LSM LSM 880 Airyscan 2 3 or 34 QUASAR GaAsP Airyscan PTC/ / QUASAR3 or 34ch Airyscan 6
7 Your Insight into the Technology Behind It 10 / LSM / LSM 880 S/N ROI LSM % S/N 29% LSM µm GFP MBD microtubule binding domain O. Samajova, Faculty of Science, Palacky University Olomouc, Czech Republic LSM 880 LSM 880 Duty Cycle (85%) 85% Sine Scan Duty Cycle 60% (60%) Equalization Field of View Difference to Linearized Scan Pixel Time Scan Line (x) x Resulting Pixel Time Signal 12% not used (12%) Scan Line (x) x 7
8 Your Insight into the Technology Behind It LSM 880 LSM / NDD GaAsP FCSFCCS LSM 880 LSM 880 descanned non-descanned 1 Typical Sensitivity of Detectors PMT GaAsPQE LSM880 Airyscan Phalloidin-Alexa546Alexa488 AP3 DAPI 3 Hela S. Traikov, BIOTEC, TU Dresden, Germany 8
9 Expand Your Possibilities Experiment Designer Experiment Designer LSM 880 ZEN LSM Repetitive Manipulation Experiments Multiposition Z-stack acquisition with individual heights Z ZENExperiment Designer Z / 9
10 Expand Your Possibilities LSM 880 LSM 880 NLO : nm 500nm nm S 1 VIS S 0 IR nm 1 SHG 800nm 1100nm 400nm 550nm SHG SHG 10
11 Expand Your Possibilities LSM CLSM Airyscan LSM 880 XY140nm Z400nm ELYRASR-SIM PALM SR-SIM XY 120nm Z350nm PALM / XY20nm Z50nm LSM 880 LSM 880 & ELYRA 11
12 Expand Your Possibilities LSM 880 Axio Examiner LD- 20x/ Scale 6 CLSM60 7 YFP-HScale Hama.et al, Nat Neurosci.2011) ZMaximum Intensity Projection H. Hama, F. Ishidate, A. Miyawaki, RIKEN BSI, Wako, Japan 12
13 Expand Your Possibilities LSM 880 ZEISS LSM 880 Axio Observer Axio Examiner LSM 880 NLO in vivo Airyscan Axio Imager LSM 880 Airyscan AiryscanZ FCS FLIM 2 GaAsP LSM BiG.2 LSM BiG.2 NDD FLIM 13
14 Expand Your Possibilities LSM 880 ZEISS LSM 880 GaAsP 2ch NDD AxioExaminer 6ch GaAsP NDD LSM BiG.2 LSM 880 Airyscan AxioCam LSM 2 µm FCS GaAsP FRETFRAP N&B 14
15 Tailored Precisely to Your Applications 488nm 140nm xy /400nm z Airyscan GaAsP Scale 5.6mm Axio Examiner/ / LSM AxioCam Airyscan GaAsP 2.5 / 2D 3D 2 FLIM 2 FERT 2 FLIM LSM BiG.2 FRET Experiment Designer LSM 880 CLSM 2 3D ELYRA S.1SR-SIM) 5 / / Airyscan GaAsPLSM 880 GaAsP / LSM 880 NLO LSM 880 NLO/OPO 15
16 Tailored Precisely to Your Applications Airyscan GaAsP5 / m Airyscan GaAsP40 m LSM 880 Airyscan3Airy VP 16
17 at Work Confocal Airyscan Confocal Airyscan LSM µm ZO-1 FITC EEA1 S. Almewadar, CRTD, TU Dresden, Germany Alexa 555 P. O`Toole and P. Pryor, University of York, UK Confocal Airyscan 2 µm 488nm 1 m OPO optical parametric oscillator 1100nm LSM BiG.2 Becker & Hickel TCSPC 17
18 at Work Confocal Airyscan Confocal Airyscan LSM 880 CNPase C. Dornblut, Leibniz Institute for Age Research FLI,Jena, Germany Confocal Airyscan 10 µm 10 µm Alexa 555 CLSM Airyscan SR P. O`Toole and P. Pryor, University of York, UK CNPase 10 C. Dornblut, Leibniz Institute for Age Research FLI, Jena, Germany 18
19 at Work Confocal Confocal Confocal LSM 880 Airyscan Airyscan Airyscan IMR90 2DNA DAPI G Alexa 488 CAlexa 546RTEL1 2 sirnartel1 2 Airyscan SR J. Karlseder Molecular and Cell Biology Laboratory and J. Fitzpatrick Waitt Advanced Biophotonics Core, The Salk Institute, La Jolla, USA. 19
20 : Your Flexible Choice of Components LSM Axio Observer Axio Examiner Axio Imager UV 355nm 405nm VIS 440nm 458nm 488nm 514nm ZEN black edition Tile & Positions Experiment Designer FRAP ELYRA 543nm 561nm 594nm 633nm FRET RICS FCS Deconvolution 3D VisArt Ti-Sa OPO 4. Z Definite Focus 3 or 34chGaAsP and/or PMT Airyscan 2. 2ch GaAsP BiG.2 6 NDD GaAsP C-APOCHROMAT 12 NDD GaAsP/PMT Plan-APOCHROMAT T-PMT W Plan-APOCHROMAT LD Plan-APOCHROMAT EC Plan-NEOFLUAR 20
21 : System Overview LSM BiG.2 2ch GaAsP ピエゾ Ｄｅｆ ｉ ｎ ｉ ｔｅｆｏｃｕｓ ステージフォーカス モジュール 拡張レーザ用制御モジュール ＵＶ / ＮＬＯ ELYRA P.1 ELYRA PS.1 ELYRA P.1 ELYRA PS.1 LSM 880 Rearport for system ＮＬＯ接続キット ピエゾ対物 フォーカス LSM BiG.2 2ch GaAsP LSM 880 メタルハライド光源 倒立顕微鏡用スキャニングステージ 130ｘ85ＳＴＥＰ Airyscan HXP120V / X-Cite 120V デジタルカメラ AxioCam 倒立顕微鏡 Axio Observer.Z1 ピエゾ対物 フォーカス or 6x laser nm LSM 880 正立顕微鏡用スキャニングステージ 130ｘ85ピエゾ LSM BiG.2 2ch GaAsP マルチフォトン用 外部検出器 ＮＤＤ デジタルカメラ ＸＹジョイスティック ピエゾ 特長 アプリケーション システム構成 システム構成 技術仕様 サービス T-PMT マルチフォトン用 外部検出器 ＮＤＤ AxioCam 光路切り替え ミラー マルチフォトン用 外部検出器 ＮＤＤ LSM 880 ＸＹステージコントローラ ピエゾ LSM LSM とは とは LSM BiG.2 2ch GaAsP コントローラ ジョイスティック付 レーザ システム制御モジュール 4x laser nm Interface for lamp ＨＡＬランプハウス１００Ｖ LSM BiG.2 2ch GaAsP LSM BiG.2 2ch GaAsP LED蛍光光源 Colibri 正立顕微鏡 Ｚ２ / Ｍ２Ｐ Ａｘ ｉ ｏｉｍａｇｅｒ Interface for lamp デジタルカメラ AxioCam 30インチＴＦＴモニタ 蛍光光源 ＨＢＯ１００Ｗ マルチフォトン用 外部検出器 ＮＤＤ LSM 880 アクティブ除振機能付システムテーブル/ＮＬＯ mm l d h or: mm l d h Ａｘ ｉ ｏｅｘａｍｉ ｎｅｒ用 スキャニングステージ LSM BiG.2 2ch GaAsP アクティブ パッシブ除振機能付システムテーブル小 mm l d h コントローラ ジョイスティック付 T-PMT 光路切り替え ミラー ＨＡＬランプハウス１００Ｖ or: アクティブ除振機能付システムテーブル大 mm l d h LSM BiG.2 2ch GaAsP リアルタイムコントロール システム 制御用ワークステーション ２ｃｈ ＧａＡｓＰ ＮＤＤ 正立顕微鏡 ｎｅｒ Ｚ１ Ａｘ ｉ ｏｅｘａｍｉ 21
22 Technical Specifications LSM 880 LSM 880 LSM 880 SP PC PC PC PC Axio Imager/Axio Examiner 22
23 Technical Specifications LSM 880 NLO LSM 880 PC LSM 880 NLO Dual Stand PC 23
24 Technical Specifications LSM 880 NLO / Axio Examiner OPO LSM 880 NLO / Axio Observer OPO LSM 880 PC PC LSM 880 NLO / Axio Examiner 2x NLO LSM 880 NLO / Axio Observer 2x NLO PC PC 24
25 Technical Specifications Physical Dimensions cm cm cm kg NLO NLO 2 LSM880NLO laser NLO or OPO NLO LSM UV Airyscan UV 200 VIS LSM 880 XY Axio Imager.Z2 Axio Examiner.Z1 / Axio Observer.Z1, SP / RP Axio Imager.Z2:<25 nm; Axio Observer.Z1: <25 nm; Axio Examiner.Z1: <30 nm; / Axio Observer Definite Focus XY 1 Axio Observer 0.2 Axio Imager 25
26 Technical Specifications LSM / / 430 / /sec ;0.1 Axio Examiner XY 20 mmaxio Examiner 18 mm 405 nm 100 BiG.2 Airyscan 3 or 34 GaAsP and/or PMT GaAsP 45% 2ch GaAsPLSM BiG.2 Airyscan 32ch GaAsP 12 NLO NDD PMT and/or GaAsP T-PMT 3 or 34 GaAsP and/or PMT 3 nm 8/12/16bit 35ch PC 26
27 Technical Specifications ZEN Smart setup Crop ROI ROI Reuse / xyz Lambda t /Smart setup 99 FRAP 99ROI / line/frame / / XY XZ/YZ OrthogonalCut 2.5DMaximum intensity projection / LUT / / LSM 880 3D VisArt ROI-HDR Physiology FRET FRAP efficiency analysis Visual Macro Editor RICS Image Correlation Experimant Designer Macro Environment 3D/4D :shadow projection transparency projection surface rendering point spred function 3 :nearest neighbor maximum likelihood contrst iterative Gain 1 / / FRET Accepter Photobleaching/Sensitized Emission FRAP PMT GaAsPPublish by Gratton / VBA 27
28 Technical Specifications LSM 880 RGB PTC ; nm V PTC ; nm VIS-AOTF Ar 458/488/514 nm 25 or 35 mw HeNe 543 nm 1 mw DPSS 561 nm 20 mw HeNe 594 nm 2 mw HeNe 633 nm 5 mw Diode Laser Pulsed/CW 405 nm 30 mw CW mode Pulse mode 15 mw mw attenuator 1/ MHz 20 MHz- 0.2 MHz- 0.5 MHz- 0.8 mw mw ps <20 ps 28 Diode Laser Pulsed/CW 440 nm 25 mw CW mode Pulse mode 15 mw mw attenuator 1/ MHz 20 MHz- 0.1 MHz MHz- 0.4 mw mw ps <20 ps
29 Technical Specifications Laser Module UV 355 nm AOM DPSS 355 nm 60 mw V 30A Hz 2100 VA LSM OPO OPO 100V 15A VA 800 VA 600 VA 2000 W 29
30 Technical Specifications LSM T = T = T = T = /h 6. 1 h3 h at m 9. 2 kw 10. with system table 5 m pp at 5 Hz 10 m pp at 10 Hz 10 m pp at 20 Hz g VISIBLE AND INVISIBLE LASER RADIATION Avoid exposure to beam Class 3 B laser product IEC : 2007 VISIBLE AND INVISIBLE LASER RADIATION Avoid eye or skin exposure to direct or scattered radiation Class 4 laser product IEC : 2007 VISIBLE AND INVISIBLE LASER RADIATION Avoid direct exposure to beam Class IIIb Laser product VISIBLE AND INVISIBLE LASER RADIATION Avoid eye or skin exposure to direct or scattered radiation Class IV Laser product 30 LSM 880 meets the requirements according to IEC :2007
31 Count on Service in the True Sense of the word ZEISS LSM 880 ZEISS >> 31
32 Printed in Japan P15 MIC 016 PR SK-11/ TM Tel Fax
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超解像顕微鏡 ZEISS LSM 8 Family with Airyscan 分解能 明るさ スピード全ての要素を向上させる唯一のハードウェア超解像 これまで 分解能 明るさ スピードはトレードオフの関係でした Airyscan は全ての要素を同時に向上させることができるため 共焦点ユーザーのイメ
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