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1 :1014: :1015: i
2 15:1015: :2016:05 7. virus associated hemophagocytic syndrome: VAHS) :0516:45 16:4517:45 Current Status of Japanese Encephalitis in Korea Haewol Cho, Ph.D. Director General, National Institute of Health, Ministry of Health and Welfare, Seoul, Korea ii
3 :0010: prm :0010:10 10:1011: Afjal Hossain Khan ( iii
4 Current Status of Japanese Encephalitis in Korea Hae Wol Cho, Ph.D. Director-General, Korea National Institute of Health The epidemiology of Japanese encephalitis (JE) in the Republic of Korea has been changed over past 20 years from 1980 to A number of recent JE cases decreased dramatically and the different aspects of JE case have been observed: In general, the disease mainly affected children aged 4 to 7 years in the past, but over recent 20 years, age distribution has shifted to over 20 years of age. The geographical distribution of JE cases in its high epidemic covered the southwestern part of the Korean peninsular, or Cholla province, but it has moved to the southeastern part of Korea, or Kyungsang province. We observed a correlation between the vector mosquitoes population and JE incidence in the seasonal distribution. In epidemic of JE in Korea over last 10 years, less than 10 cases were reported. The annual disease cases dropped under 5 since There are many factors to explain this fact. Vaccination has been applied to the group (age of 3-14) since Presumably, vaccination of JE-susceptible people is an effective and practical way to control JE in Korea. 1
5 Detection and characterization of viruses from the field-collected mosquitoes in Japan Hoshino, K., Isawa, H., Sasaki, T., Tsuda, Y., Higa, Y., Takasaki, T., Kotaki, T., Kobayashi, M., Yano, K., Sawabe, K. ( 1 Department of Medical Entomology, National Institute of Infectious Diseases, 2Department of Virology, National Institute of Infectious Diseases) WN , C6/36 Vero BHK (CPE) RT-PCR alphavirus bunyavirusflavivirus TaqManRT-PCR WN 20 7,
6 Blood source animals of Japanese mosquitoes susceptible to West Nile virus, and molecular identification for Culex pipiens group. Sawabe, K., Isawa, H., Higa, Y., Kasai, S., Hoshino, K., Sasaki, T., Tsuda, Y., Kobayashi, M. (Department of Medical Entomology, National Institute of Infectious Diseases) WN WN WN WN 110 WN 34 WN ACE rdna ITS1 WN WN WN
7 4
8 Surveillance of West Nile virus in mosquitoes in Osaka Prefecture. Ikuko Aoyama, Takahiro Yumisashi, Kazuo Takahashi, Toru Otake, and Yoshinobu Okuno Department of Infectious Disease, Osaka Prefectural Institute of Public Health 5
9 Examination of mosquito larvae and adult in winter season Masahiro Yoshida1, Tosio Yamasita1, Mutuo Kobayashi2 ( 1 Laboratory of Bio-research, 2 Department of Medical Entmology National Institute of Infectious Diseases ) 6
10 1 A proposal for West Nile fever project from entomologists of Japanese encephalitis vector Osamu Maeda 1 and Mitsuo Takahashi 2 1 Kyoto City Institute of Public Health, 2 National Institute of Health, Former position viremia HI 10km viremia
11 /1001/1000 viremia D. E a. b C. H. viremia A.. 10 B. D E. F. 8
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13 VAHS4 JEV antibodies in four patients diagnosed as virus associated hemophagocytic syndrome (VAHS) who lived in Kurahashi islands. Tomohiko Takasaki Reiko Nerome Masaru Kuwayama Youzo Uchida Tetsuo Nishiura Shunji Matsuda Ichiro Kurane National Institute of Infectious Diseases Hiroshima Prefectural Institute of Health and Environment National Hospital Organization Kure Medical Center National Hospital Organization Ehime National HospitalPresent address VAHS HI
14 Sero-prevalance of Boar in Chugku District for Japanese encephalitis Tomohiko Takasaki 1, Chang Kweng Lim 1, Kyoko Sawabe 2, Masaru Kuwayama 3, Noboru Kishi 4, Ichiro Kurane 1 1National Institute of Infectious Diseases, Virology I 2National Institute of Infectious Diseases, Medical Entomology 3 Hiroshima Prefectural Livestock technological research center 4 Hiroshima Prefectural Institute of Health and Environment 11 IgG ELISAIgM ELISA IgG IgG ELISA 96 2 POD IgG 640 Cut off IgM IgM IgG Index;1.5 Indx: IgG IgG
15 Vector Survey of Japanese encephalitis on Kurahashijima Island, Hiroshima, Japan Yoshio Tsuda 1, Kyoko Sawabe 1, Yukiko Higa 1, Keita Hoshino 1, Haruhiko Isawa 1, Toshinori Sasaki 1, Mutsuo Kobayashi 1, Masaru Kuwayama 2 ( 1 Department of Medical Entomology, National Institute of Infectious Diseases, 2 Hiroshima Prefectural Institute of Health and Environment) ,
16 10 3'- 1 Peter W. Mason A deletion of nucleotides in the 3'-untranslated region of Japanese encephalitis virus genome found in recent isolates induces growth-restriction in cultured cells Tomohiro Ishikawa 1, Peter W. Mason 2, Shigeru Tajima 3, Reiko Nerome 3, Tomohiko Takasaki 3, Ichiro Kurane 3, Eiji Konishi 1 1 Department of Health Sciences, Kobe University School of Medicine, 2Sealy Center for Vaccine Development, University of Texas Medical Branch, 3Department of Virology 1, National Institute of Infectious Diseases RNA BHK C6/36 BHK ^2 FFU/ml 7.410^7 FFU/ml 72 C6/36 BHK ^6 FFU/ml ^7 FFU/ml 48 C6/
17 RNAi JEV Inhibition of JEV in mice by RNAi 13
18 12 prm Involvement of conserved region of flavivirus prm protein in virus particle budding Kentarou Yoshii 1, Akiko Goto 1, Junko Nio 2, Mayumi Obara 1, Kazue Kawakami 1, Hiroaki Kariwa 1, and Ikuo Takashima 1 ( 1 Laboratory of Public Health, and 2 Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University) endoplasmic reticulum : ER trans-golgi network prm/e TBE Oshima5-10 prm/e subviral particles : SPs prm prm pr- TBE SPs SPs SPs ER SPs SPs prm pr- cdna SPs 14
19 Analysis of mouse anti-dengue type 2 virus monoclonal antibodies showing antibody-dependent enhancement of infection and/or neutralizing activities Atsushi Yamanaka, Eiji Konishi (Department of Health Sciences, Kobe University School of Medicine) Vero 90% K ABC 30 2 (6 )(1 )(3 ) (10 ) 4 IgG2a IgG1 IgG2a IgG2b IgG 0.01mg/ml IgG (0.01mg/ml) 15
20 16
21 15 Afjal Hossain Khan New strategy of live attenuated West Nile virus vaccine development Afjal Hossain Khan, Isao Fuke, Toyokazu Ishikawa, Shingo Inoue, Kouichi Morita Department of Virology, Institute of Tropical Medicine, Nagasaki University 2 Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University WNV 1999 WNV WNV WNV WNV WNV Dr. Monath D DNA WNV ML-17 WNV NY-99 PCR in vitro transcription RNA C6/36 WNV 3 C57BL ~10 FFU 1 100FFU 250LD 50 WNVNY ~10 7 FFU ML-17 WNV 17
22 18
23 17 DNA Combined immunization of flavivirus DNA and protein vaccines: Cross-immunogenicity between dengue viruses and Japanese encephalitis virus in mice Jun-ichi Imoto, Eiji Konishi Department of Health Sciences, Kobe University School of Medicine JEV DNA DNA (JEVAX) JEV 2 DENV2 90% 1: :20-1: ddy 10 µg 2 DNA pcd2me 1/10 JEVAX 3 9 DENV2 1:10 1:201:20 1:40 P< µg pcd2me DENV2 10 µg DNA 1 6 Balb/c 100 µg 4 DNA 1/10 JEVAX DNA 19
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