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12 Helicobacter pylori Helicobacter pylori Helkobacter pylori erythromycin (EM) Mueller Hinton broth (Difco) H. pylori Staphylococcus aureus ATCC Escherichia coli ATCC25922 amoxicillin (AMPC), clarithromycin azithromycin (CAM), (AZM) ciprofloxacin (CPFX) Helicobacter pylori H. pylori H. pylori amoxicillin (AMPC) clarithromycin (CAM) H. pylori erythromycin (EM), roxithromycin CAM, (RXM), azithromycin (AIM) rokitarnycin (RKM) clavulanic acid/amoxicillin fropenem (FRPM) levofloxacin (LVFX)

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14 K. pneumoniae(105) 2%, 1%, E. cloacae(98) 40%, 0%, S. marcescens(86) 21%, 10%, C. freundii(40) 50%, 0 %, P. aeruginosa(446) 12%, 15%, E. faecalis(211) 0%, E. faecium(72) 82%,E.

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23 Immunochromatography polymerase chain reaction

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44 Protective effect of rhg-csf on Trichosporon gell infection in immunosuppressed bei mice Second Department of Internal Medical University. School of Medical Sciencies, Nagasaki University* OP. Kamberi, E. Yamagata, H. Nagai, A. Hashimoto, Y. Goto, T. Tashiro' and M. Nasu Medicine,Oita The effect of rhg-csf alone and in combination with conventional antifungal therapy on the course of disseminated trichosporonosis in neutropenic mice was investigated. ethods. Cyclophosphamide N plus prednisoloneimmunosuppressed mice were inoculated iv with 6x106 CFU of T.beigellii 239,and divided into groups of 12 mice each to receive rhg-csf or and fungal drug. rhg-csf was given sc once daily at30, 60, or 120ƒÊg/kg for 5 days. Ampho ter icin B (MPH, mg/kg),fluconazole(flcz,10mg/kg) or itracona- 1 zole(icz,20mg/kg) was given alone for 7 days or in combination with 60ƒÊg/kg of rhg-csf.three mice per each group were sacrificed on day 4 for quantitative tissue counts of lung(l) and kidney (K).Nine remaining mice in each group were observed for survival for 21 days postinfection. fesults. rhg-csf was protective at 60 and 120 ggikep<0.05 and P<0.01 vs control). Both doses reduced significantly fungus titers in L(P<0.01 vs control) and K(P<0.01 vs control).rhg-csf + either FLCZ or AMPH prolonged survival and signi ficantly reduced tissue burdens, with the former combination being superior.rhgcsf + ICZ resulted in no significant enhancement of the activity. Conclusiaa The administration of rhg-csf to neu tropenic mice augmented resistance against )7 eigelii infection.the effectiveness of combina- b tion rhg-csf + antifungal drug was depend on antifungal drug used.

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46 1)Hatano K, et a1.: Chemother.(Basel) 40: 1-7, )Ford C W, et al.:j.med.microbio1.28: , 1989

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80 Bilophila wadsworthia Bilophila wadsworthia E. coli 20 S. agalactiae P. anaerobius B. fragilis P. bivia cefditoren (CDTR) Acefdinir (CFDN) A cefteram (CFTM) Acefotiam (CTM) Acefpodoxime

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105 Enterococcus casseliflavus E. casseliflavus TUH327 cassellfavus

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Table 1. Antibacterial activity of cefdinir, cefixime, cefteram, cefuroxime, cefaclor and amoxicillin against standard strains Inoculum size: 108 cells/ml CFDN: cefdinir, CFIX: cefixime, CFTM: cefteram,

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