ACQUITY UPLC I-Class/Xevo TQD Mark Roberts and Michelle Wood Waters Corporation, MS Technologies Centre, Manchester, UK ACQUITY UPLC I-Class/Xevo TQD 2 2 MRM 178 / 950 MS ACQUITY UPLC/ACQUITY TQD 2 1-3 MRM 100 LC/MS LC/MS 2 ACQUITY UPLC I-Class/Xevo TQD MRM 1-3 ACQUITY UPLC I-Class/Xevo TQD ACQUITY UPLC I-Class System Xevo TQD RADAR Technology PIC Scannning ACQUITY UPLC HSS C18 Waters UPLC /MS/MS 1
Sigma-Aldrich (Poole,Dorset,UK) LGC Standards(Teddington,Surrey,UK) 1 mg/ml Sigma-Aldrich Greyhound Chromatography(Brikenhead,UK) / Waters ACQUITY UPLC I-Class/Xevo TQD ACQUITY UPLC / TQD UPLC MS ACQUITY UPLC I-Class Xevo TQD A B ACQUITY UPLC HSS C18 2.1 x 150 mm 1.8 µm 186003534 50 10 5 µl MRM 10 µl 5 mm ph 3.0 5 mm ph 3.0 400 µl/min 5 mm ph 3.0 0.1% B 13% 95% 15 ESI ESI 3.0 kv 400 800 L/h 20 L/h MassLynx Multiple Reaction Monitoring MRM Targetlynx TM ChromaLynx TM ACQUITY TQD 1,2 ACQUITY UPLC 1,2 ACQUITY UPLC I-Class/Xevo TQD 2
FTN 5 ACQUITY UPLC I-Class 1 UPLC 100 µl RADAR PIC Xevo TQD 6 1 RADAR MRM Product Ion Confirmation scanning (PICs) MRM 1 ACQUITY UPLC I-Class/Xevo TQD MRM MS 178 MRM / 30 0.5 0.5 ACQUITY UPLC MRM ACQUITY UPLC I-Class ACQUITY UPLC I-Class/Xevo TQD 3
+20 V +35 V +50 V +65 V +80 V +95 V -20 V ChromaLynx 950 ACQUITY TQD Xevo TQD MRM SSM 2 ACQUITY UPLC I-Class/Xevo TQD SSM TargetLynx SSM 10 MRM 0.3 ACQUITY UPLC I-Class ACQUITY UPLC I-Class/Xevo TQD 4
Total Ion Chromatogram Library Spectrum Acquired Spectrum Candidate Listing 3 SSM ChromaLynx 10 MRM 1 0.19 ACQUITY UPLC I-Class methadone EDDP cocaine benzoylecgonine 4 ACQUITY UPLC I-Class/Xevo TQD MRM TargetLynx ACQUITY UPLC I-Class/Xevo TQD ACQUITY UPLC I-Class ACQUITY UPLC I-Class/Xevo TQD 5
ACQUITY UPLC/TQD (original configuration) ACQUITY UPLC I-Class/Xevo TQD (new configuration) RT RT RT Methadone 8.61 8.77 434734 8.80 463948 EDDP 7.46 7.67 145310 7.65 149118 Paracetamol 1.50 1.58 5703 1.55 55966 Cocaine 4.61 4.80 11644 4.75 11702 Benzoylecgonine 2.97 3.13 10500 3.09 11261 Nicotine 1.01 1.02 3284 1.05 6143 Caffeine 2.10 2.19 1499 2.16 3866 Temazepam 9.34 9.46 3683 Oxazepam 8.07 8.17 1853 8.18 2528 Theophylline 1.46 1.4 5 2255 Nordiazepam 9.14 9.31 1297 1 MRM 4 methadone TargetLynx ACQUITY UPLC I-Class/Xevo TQD 6
MRM 950 ChromaLynx 5 ACQUITY UPLC I-Class/Xevo TQD mirtazapine mirtazapine N-desmethyl ChromaLynx mirtazapine N-desmethyl ACQUITY TQD ACQUITY TQD Xevo TQD xylometazoline MRM 5 mirtazapine mirtazapine N-desmethyl ChromaLyn ACQUITY UPLC I-Class/Xevo TQD 7
PICs hydromorphone morphine Xevo TQD MRM Product Ion Confirmation scanning(pics) 6 MRM hydromorphone hydromorphone hydromorphone morphine MRM Hydromorphone morphine MRM 0.2 PIC PICs morphine hydromorphone TargetLynx hydromorphone morphine B A C 6 TargetLynx hydromorphone (A) 200 ng/ml hydromorphone (B) morphine (C) ACQUITY UPLC I-Class/Xevo TQD 8
RADAR RADAR MRM MRM RADAR MRM MRM RADAR MRM mirtazapine N-desmethyl desmethyl citalopram xylometazoline 3.79 6.47 7.42 8 2 30 V RADAR 7 RADAR MRM MRM mirtazapine N-desmethyl 3.79 desmethyl citalopram 6.47 xylometazoline 7.42 A B 8 RADAR mirtazapine N-desmethyl 3.79 (A) xylometazoline 7.42 (B) 30 V ACQUITY UPLC I-Class/Xevo TQD 9
UPLC/MS ACQUITY UPLC I-Class MS RADAR PICs Xevo TQD 1. Roberts M, Lee R, Wood M. Targeted MRM Screening for Toxicants in Biological Samples by UPLC/MS/MS. Waters Application Note 720002749EN. 2. Lee R, Roberts M, Paccou A, Wood M..Development of a New UPLC/MS Method for Systematic Toxicological Analysis. Waters Application Note 72002905EN. 3. Humbert L, Grisel F, Richeval C, Lhermitte M. Screening of Xenobiotics by Ultra-Performance Liquid Chromatography Mass Spectrometry Using In-Source Fragmentation at Increasing Cone Voltages: Library Constitution and an Evaluation of Spectral Stability. Journal of Analytical Toxicology. 2010; Vol. 34. 4. Rosano TG, Wood M, Swift TA. Postmortem Drug Screening by Non-Targeted and Targeted UltraPerformance Liquid Chromatography-Mass Spectrometry Technology. Journal of Analytical Toxicology. 2011; Vol. 35. 5. ACQUITY UPLC I-Class System Brochure. Waters Brochure 720003290EN. 6. Xevo TQD Brochure. Waters Brochure 720003953EN. www.waters.com 140-0001 1-3-12 5 TEL 03-3471-7191 FAX 03-3471-7118 532-0011 5-14-10 11F TEL 06-6304-8888 FAX 06-6300-1734 Waters ACQUITY UPLC ACQUITY UPLC PIC Xevo MassLynx Waters Corporation RADAR TargetLynx ChromaLynx The Science of What s Possible Waters Corporation 2013 Waters Corporation. Printed in Japan. 2013 6 720004602JA PDF