ver. 3.3JT
EVENING PRIMROSE EXTRACT 1 - PMS 1
2 1 4 Oenothera laciniata Oenothera striata Oenothera biennis Oenothera erythrosepala - 2 80 60 40 20 0 1. 2
3 PGGPAC 2. 3
4 ESR3.5 10 5 /g 4
3 1 Helicobacter pylori H.pylori 1983 2 Osaki et.al., J Med Microbiol.2002 NH3 40 80 6000 9 3 PPI 2 MACH 1 study 85% 50.5 13.7% 8.8% 2.6% 1.4% 3.9% 5
4 3 SOD VacA PAI Type CagA LPS NapA IL-6 IL-8 DNA 4. 3. 6
4 1 MIC 64µg/ml 0.0064 6480 100 100 ATCC43503 ATCC43504 ATCC43579 ATCC49503 NCTC1163 TK1003 TK1008 TK1021 TK1022 TK1023 TK1025 TK1029 TK1030 TK1036 TK1042 TK1402 KR2001 KR 2002 KR 2003 KR 2005 KR 2007 KR 2009 KR 2067 KR 2093 24 64 64 64 123 >128 >128 5000 5000 0 20 40 60 80 100 120, 4. µ /ml 80 100 100 7
PGG 64 PGG 16 32 PAC 64 >128 >128 0 20 40 60 80 100 120 µ /ml 5. 6 Y X h0µg/ml 512 256µg/ml 2 128 64 32µg/ml 24 10 8 10 7 Log of CFU/ml 10 6 10 5 10 4 10 3 0µg/ml 512µg/ml 256µg/ml 128µg/ml 64µg/ml 32µg/ml 10 2 10 0 2 4 24 h 6. H.pylori H. pylori TK1402.7 BHI (512,256,128,64,32,0µg/ml).0,2,4,240.1ml. 8
2 ph IC50 8.54µg/ml 100 (%) 80 60 40 20 0 0 10 20 30 40 50 (µg/ml) 7. 100 3 16µg/ml 82.45 9
8 Y X 16 32 64µg/ml negative control(mkn45 cell only) positive control(0%) 64µg/ml 32µg/ml 16µg/ml Osaki et.al.,j Med Microbiol. 2002 Fluorescence intensity 8. H. pylori TK1402( TK1402 ).7 BHI 5 10 CFU/mlPKH-2 (64,32,16µg/ml) TK1402 ( MKN45 ) 30 TK1402 MKN45 1. H. pylori MKN45 (FCM). 1. H.pylori µg/ml 64 15.12 61.51 (98.10) 32 54.23 230.86 (98.18) 16 139.56 236.01 (82.45) 0 795.03 594.44 (0). MKN45 A MKN45 onlya MKN45 T 1 T A A )( ) 10
4 MGS/Sea,, TK1402 ( 9 CFU/ml)mg/ml (/,, ) 1 H.pylori 16SrRNA RT PCR(reverse transcription-polymerase chain reaction) H.pylori 9 16S rrna c 5 4 b 5 5 4 16S rrna a b c d e f 9. 16S rrna RT-PCR () H.pylori 11
5 ddy 240mg/kg 7 3 10 5 S.D. Log of CFU/stomach 10 4 10 3 10 2 10 1 Con 15 30 60 120 240 mg/kg 10. ddy H. pylori ddy 4,10 8 CFU/ml H.pylori 0.5ml 12 24 22 1 7 15 30 60 120 240 mg kg 1 1 1 H.pylori 6 12
6 2 ( cont. ) 41.0 500mg/kg ( 500mg/kg ) 1.6 mm) 2 80 70 S.D. 60 50 40 30 20 10 0 500mg/kg cont. 500mg/kg 11. 24 ( istar)( 280 300g) 2 100 0.5ml10mg 30 2 8ml 10 (mm 2 ) Scion Image 5 2 30100 0.5ml (cont.)5 13
7 cont. 10 10 10 9 10 8 10 7 Log of CFU/ml 10 6 10 5 10 4 10 3 cont. 32 µg/ml 64 µg/ml 128 µg/ml 256 µg/ml 512 µg/ml 10 2 10 0 5 10 15 20 25 h 12. 14
UBT 45 40 35 30 25 20 15 10 5 0 8 -PH120mg 5 840mg 4 -PH 2 8 / 13 C 13 CO 2 13 C 13 CO 2 UBT UBT 13 2 UBT 1 2 50 45 40 35 30 25 20 15 10 5 0 1 2 13. ELISA 14 2 15
3 1.5 2.5 2 1.5 1 1 0.5 0.5 0 0 1 2 1 2 14. / / / / 15 2 / 4.5 4.5 / 4 3.5 3 2.5 2 4 3.5 3 2.5 2 1.5 1 2 1.5 1 2 15. / 16
5. 1 100 80 60 40 20 0 0 20 40 60 60 100 120 140 16. 100 2 ph 100 80 60 40 20 0 3 4 5 6 7 8 9 10 11 12 1 1 1 ph 17. ph 0.05 80 ph7 100 17
6. PH 90 120mg/WSPH 180 240mg/ 7. -PH -WSPH 1.5g 100g 2.0 g 100g 2.2 g 100g 2.6 g 100g 1.6 g 100g 0.1 g 100g 1.2 g 100g 6.4 g 100g 93.2 g 100g 86.1 g 100g 0.3 g 100g 2.8 g 100g 4.8mg 100g 29.9mg 100g 396kcal 100g 356 kcal 100g -P WSP -P 12 8 25 300080303-002 -WSP 13 6 21 301060141-001 18
8. 1 BHC 0.02ppm DDT 0.02ppm 0.01ppm 0.01ppm 0.01ppm 0.05ppm 0.05ppm 0.05ppm 2 8 24 300080303-001 2 5SD 17 5000mg/kg 22 3 50 20% 14 0.5% (LD50) 5000 mg/kg 3 (CHL/IU) 75 106.1 150 212.1 300µg/ml 100 141.1 200 282.8 400µg/ml 24 39.5 59.3 88.9 133.3 200µg/ml 48 19.8 29.6 44.4 66.7 100µg/ml 5 CHL/IU 19
4 Ames Salmonella typhimurium( ) Esherichia coli() WP2uvrA Ames 5 1 7 200mg/kg 2 100 50mg 24 48 9. 10. -PH -WSPH 5kg 20
11. 12. 21
1. Folin-Denis 4. H. pylori 5 19 24 7 (Brucella Agar) (O 2 5,CO 2 10, 2 85 ) 10 (FCS) (Brucella Broth) 24 10 FCS H.pylori 37,O 2 5,CO 2 10, 2 85 5. 2 6. H.pylori H. pylori TK1402 7 (Brain Heart Infusion BHI) (512, 256,128,64,32,0µg/ml).0,2,4,24 0.1ml 7. 0.02 /ml 50µ 37 15 400 mm 100 mm 300µ 37 15 100µ 5.0 25mg 500 ml 2.5 ml 300 ml 2.2 2.5 10 3.0 ml 500 ml 2.5 ml 65 20 25 630nm 8. H. pylori TK1402 (5 10 8 CFU/ml) PKH-2 (MKN45 ) (FCM) 22
9. 16SrRNA RT-PCR H.pylori MGS/Sea,9, H.pylori H. pylori TK1402 (1 3 10 9 CFU/ )mg/ml (/,, ) 1 H.pylori 16SrRNA RT PCR(reverse transcription-polymerase chain reaction) H.pylori 10. ddy H. pylori ddy 4,10 8 CFU/ml H.pylori 0.5ml/ 12 24 22 1 7 15,30,60,120,240mg/kg 1 1 1 H.pylori 6 11. 24 ( istar)( 280 300g) 2 100 0.5ml 10mg 30 2 8ml 10 (mm 2 ) Scion Image 5 2 30 100 0.5ml (con) 5 12. NBRC NO.12005 Lactobacillus brevis MRS-broth 24h 0.1ml BCP 48h 13. 14. 15. / -PH120mg 5 840mg 4 -PH 2 8 / 23
Oenothera biennis 18.0 18.0 Folin-Denis 5.0 1 g 105 2 ) 1 10 ppm 2 1 ppm 1 10 3 g 1 10 2 g BGLB 33 67 100 24
Oenothera biennis 15.0 15.0 Folin-Denis 5.0 1 g 105 2 ) 1 10 ppm 2 1 ppm ( ) 1 10 3 g 1 10 2 g BGLB 33 67 100 25
493-8001 TEL(0586)86-5141( ) FAX(0586)86-6191 URL/http://www.oryza.co.jp/ E-mail: info@oryza.co.jp 101-0041 1-24-10 5F TEL (03)5209-9150 FAX (03)5209-9151 E-mail: tokyo@oryza.co.jp 2003 10 7 2007 12 4 26
27