217/18 217 48 1. 2 AH1pdm9 AH3 B HI AH1pdm9 B AH3 D151GD151N HA 6 AH1pdm9 AH3 B 4 5 5 5 217/18-1 -
217 1 218 3 76 24 well MDCKLLC-MK2HEp-2HeLa RD-18sVero Caco-2 217/18 3 1).75% HI A(H1N1)pdm9 B 3 A(H3N2) 2 QIAamp Viral RNA Mini Kit QIAGEN RNA OneStep RT-PCR Kit QIAGEN NA RT-PCR PCR QIAQuick PCR Purification Kit QIAGEN DNA BigDye Terminator v3.1 Cycle Sequencing Kit Applied BiosystemsBigDye XTerminator KitApplied BiosystemsApplied Biosystems 35 MEGA6 NJ AH1pdm9 NA H275Y 1) AH3 NA E119VD151VQ226HG248RK249ER292KN294S 1,3,4) B NA R15KD197ED197NI221TN294SG47S 1) - 2 -
217/18 217 48 1. 218 3 3. 1 5) 1 2 6) 8 7 6 5 4 3 2 1 217/18 76 68 AH1pdm9 19 27.9%AH3 23 33.8%B 25 36.8% B 1 1.5% 3-3 -
B Yamagata 36.8% B Victoria 1.5% A H1pdm9 27.9% A H3 33.8% 2 217/18 6 5 4 3 2 B Victria B Yamagata AH3 AH1pdm9 1 3 217/18 AH1pdm9 AH3 B B 25:1 3:1 7) - 4 -
AH3 D151GD151N NA 8) HI AH3 23 A/Hong Kong/481/214 HI 2 128 1% 7) 3 1 AH1pdm9 23 22 B AH1pdm9 A/Bangladesh/25/217 A/Philipp ines/5/2 A/Singapore/GP198/215 2 18/19 WHO A/Michigan/45/215 9) 5 AH3 216/17 A/Hong Kong/481/214 218/19 A/Singapore/INFIMH-16-19/216 6 B 218/19 B/Maryland/15/216 7-5 -
A(H1N1)pdm9 NA A(H3N2) NA B NA 18-218 217/18-6 -
7% 1) :, 3, 26 9 http://www.nid.go.jp/niid/images/lab-manual/influenza214.pdf 218 8 1 2),,,:,6,61-66,29 3) Monto,A.S.,McKimm-Berschkin,et al.:antimicrobial Agents and Chemotherapy.,5,2395-242,26 4) Sheu,T.G.,Deyde,et al.:antimicrobial Agents and Chmotherapy.,52,3284-3292,28. 5) : http://www.city.niigata.lg.jp/iryo/kenko/yobou_kansen/4kansen/43shuho_1.htm l218 8 1 6) ; http://www.mhlw.go.jp/stf/seisakunitsuite/bunya/kenkou_iryou/kenkou/kekkaku-ka nsenshou1/houdou.html218 8 9 7),;217/18 3 6 15 https://www.niid.go.jp/niid/images/idsc/disease/influ/fludoco1718.pdf 218 8 9 8) Yi Pu Lin. et al. Neuraminidase Receptor Binding Variants of Human Influenza A(H3N2) Viruses Resulting from Substitution of Aspartic Acid 151 in the Catalytic Site: a Role in Virus Attachment?.Journal of Virology. 21,vol. 84, no. 13, p 6769-6781. https://jol.asm.org/content/84/13/672, ( 218.9.6). 9) WHO;Recommended composition of influenza virus vaccines for use in the 218-219 northern hemisphere influenza season http://www.who.int/influenza/vaccines/virus/recommendations/2182_recommenda tion.pdf 218 8 16-7 -
- 8 -
μl μl μl - 9 -
G G G G - 1 -
- 11 -
- 12 -
- 13 -
AA AA AA AA AA 1) 28 AA LC-MS/MS 2) AA AA 1 mg/ml AA1,2,3-13 C31 mg/ml Waters Sep-Pak AC-24 mgsep-pak C181 g 1 LCWaters Acquity UPLC H-class MSWaters Xevo TQD Waters:HSS C181.8µm 2.1 1mm 4 1 2.µL A B.4mL/min 6.5 Time(min) 1.5 2. 3. A 99 99 5 99 B 1 1 95 1 ESI+ 1.5kV 12 L/hr 15-14 -
MRM m/z CV(V) CE(eV) 72.1 55. 26 8 44.1 26 12 13 C3 75.1 58.1 26 8 45.1 26 12 5 3) AA AA AA 1 AA AA 4 AA C18 AC-2 AA 1 2 AA 2-12-2 AA 2 AA AA - 15 -
15 ng/g N.D. 15 ng/g N.D. 2 4 6 8 1cm 2 ng/g 6 16 32 8 2 1 8 2 5W ng/g N.D. N.D. 3 4 6 18 ng/g 87 18 49 1 2 3 5 18 5W 5g ng/g 59 17 18 17 4 2 ng/g 6 66 1 4 5 2 ng/g 2 4 77-16 -
3 ng/g N.D. 1 5W ng/g N.D. 1 3 4 5 2 ng/g N.D. 9 28 61 2 ng/g N.D. 2 15 ng/g 88 4 18 ng/g N.D. 1 2 4 6 ng/g 4 1 8 18-17 -
ng/g 9 8 7 6 5 4 3 2 1 AA AA 3-13-2 AA 2 4 6 8 1cm ng/g N.D. N.D. N.D. N.D. 2 4 6 8 2 ng/g 6 16 32 8-18 -
3 4 6 8 ng/g 25 66 76 3 4 6 8 18 ng/g 87 18 49 1 2 3 5 ng/g 4 14 12 12 1 2 3 5 18 5W 5g ng/g 59 17 18 17 1 3 9 ng/g N.D. N.D. 11 1 3 9 18 ng/g 3 8 11 1 3 9 ng/g 4 5 25 1 3 9 18 ng/g 6 22 7-19 -
AA 4 AA 3 1 1 2AA 25 2 1 ng/g 15 1 5 1 AA AA AA 1)IARC 2) 28 3) - 2 -
4 23 3 31 B1 B1B2G1G2 23 8 16 HPLC LC/MS LC-MS/MS (1) B1B2G1G2 25µg/mL LC/MS Inert Sep VRA-3GL (2) (3) 1 µg/kg 4 µg/kgn=1 a. 5 2 b. - 21 -
5. 9:1 2 ml 3mm 1) 3mm 3 33 rpm 1 4 ml (4) (3) a. 2.2 ml 2. ml 45 (5) 1. ml LC-MS/MS 3:2 1 1 (6) 1-1 1-2 1-3 LC ACQUITY UPLC-H Class MS Xevo TQD Waters 1-1 LC MS-Tune Waters:BEH C18 1.7µm 2.11 mm 4 A5mmol/L B5mmol/L.3 ml/min 5 µl Time(min) 3. 6. 7. A 6 6 3 6 B 4 4 7 4 1-2 MS-Tune ESI+.75kV 1L/hr 55 33L/hr 15 1-3 MRM Cone CE RT ESI (V) (ev) B1 312.3 + 313 > 241 313 > 213 4 4 3.96 B2 314.3 + 315 > 259 315 > 287 4 4 3.14 G1 328.3 + 329 > 243 329 > 2 4 4 2.55 G2 33.3 + 331 > 245 331 > 189 4 4 2.7 (1) 2-22 -
.5 ng/ml 1 IDL3IQL1.251.43 ng/ml 1. µg/kg.5 ng/ml.251 ppb R2.999 2 B1 B2 G1 G2.5 ng/ml.5.476.53.5 ( ng/ml ).29.25.4.25 IDL( ng/ml ).86.76.121.75 IQL( ng/ml ).286.253.43.251 5.72 5.3 7.61 5.1 R 2.251 ng/ml.9996.9993.9993.9999 (2) a. 5 4 3 5 3 5 B1 B2 G1 G2 16.9 91.3 13.2 85.1 96.6 99.8 9.4 9.2 88.6 92.2 1 11.9 87.3 94.9 81.1 91.3 2 99.7 96.1 93. 94.3 95.8 87.3 82.3 76.2 66.7 78.1 5 94.9 93.7 1.8 92.2 95.4 b. 4 4 B1 B2 G1 G2 16.9 91.3 13.2 85.1 96.6 3mm 15.1 88.5 99.8 86. 94.8-23 -
(3) (2) 2.5 µg/kg 1µg/kg 2 2 5 5 5 B1 B2 G1 G2 91.5 89.6 84.7 87.8 711 3.5 4.3 4.4 8.7 2 4.5 6.5 6.3 8.8 3 4 Inert Sep VRA-3 LC-MS/MS 1) 5 212 p49-65 - 24 -
LC-MS/MS 14 3 1 1 1) α- β- 3 HPLC LC-MS/MS α- β- (1) 3 (2) α-β- 2 LC/MS (1) (2) 4-25 -
(3) 1 1 (µg / g) α.2 β.2 ND ND ND.2.2.2.2.2.2.2.1 (4) (2) (3).2 µg/g 2 1 2 5 1 1) 17 11 29 11291 5. 5 ml 3,rpm1 1 ml (5) 2-1 2-2 2-3 LC ACQUITY UPLC-H Class MS Xevo TQD Waters 2-1 LC Waters:BEH C18 1.7µm 2.1 1 mm 5 A.5 B C5mmol/L.4 ml/min 1 µl Posi Time(min) 1. 3.5 8. 1. 13.5 A 92 92 8 3 1 92 B 8 8 2 7 99 8 Nega Time(min) 1. 3.5 8. 1. 13.5 C 92 92 8 3 1 92 B 8 8 2 7 99 8-26 - 1 ml Oasis HLB 4.4 ml 1.6 ml 2. ml Filter.2 µm LC/MS/MS 1 ml 1 ml 1 ml 1 ml
2-2 MS-Tune ESI().751. kv 12L/hr 65 33L/hr 15 2-3 MRM ESI m/z Cone CE RT (V) (ev) α- + 271 199 35 25 271 253 35 25 8.87 β- + 271 199 35 25 271 253 35 25 8.67 + 321 277-4 3 321 33-4 3 8.92 (1) 3.2 ng/ml 2 ng/ml 1 IDL3IQL1 3 - - ( ng/ml ).193.191 1.9 ( ng/ml ).73.141.167 IDL ( ng/ml ).219.423.52 IQL ( ng/ml ).73.141 1.67 () 3.78 7.38 8.81 R 2 ng/ml.999.51..999 (.51.).2 ng/ml 2 ng/ml.999 11 N=1.2 µg/g.25 ng/ml.5 ng/ml 1. ng/ml1 ng/ml1 ng/ml R2.999 (2) 4.2 µg/g 5.1 µg/g.2 µg/g S/N 1 7 12-27 -
4.1 µg/g 1.2 µg/g 2 α- 9 76 86 64 73 81 73 2 19 88 11 β- 72 75 76 58 6 74 72 2 84 72 79 6 78 74 3 3 3 3 1 1.25 ng/ml2.625 ng/ml 2.25 ng/ml 3 S/N 1 (3) 5 LC-M/SMS ME STD2 STD111 STD1 2 STD2 5 ME ME α- β- ng/ml.5-43.7-53.5-42.2-44.3-93.7-97.7-98.7.1-46.6-48.8-48. -33.4-49.8-4.6-52.8.25-37.9-31. -21.5-44.7-34.3-36.4-31.7.5-3.8-26.2-34.2-42.8-4.2-28.7-29.1-39.7-39.9-36.5-41.3-54.5-5.8-53.1 3 α- S/N 1 (4) 6 (1)(3) α- 2.2 ppm 712 25 3 β-2 51 63 7-28 -
3 6 2 - - - - - - 51 63 88 8.2 4.8 8.5 8.2 9. 8.4 69 7 76 86 12 112 8.2 4.6 13. 6.6 5.3 6.2 15.7 24.8 12.7 1.6 9.4 19.4 66 66 73 81 93 11 6. 1.3 5.3 5.1 4.9 6.3 13.2 21. 7.8 8. 11.9 17.9 73 66 75 76 13 12 8. 15.3 9.4 4.4 8.8 9.6 15.4 22.4 9.7 1.1 8.8 11.2 HPLC 4 α-β- LC-MS/MS 3 β- 1 27 4-29 -
LC-MS/MS TTX TTX LC-MS/MS TTX 1 TTX LC-MS/MS 1 ) 2 ) LC-MS/MS (1) TTX 1mg 1mL 75151 LC/MS (2) JIS3214211 (3) 7 HLB (2mg)PRiME HLB (2mg) ODS (1g) AmiconUltra-4 centrifugal filter Device 4mL 1K NMWL) 1 ODS(36mg) GC5mg 2AC2-3 -
1 MILLIPORE 2 GL Waters (4) Waters Xevo TQD LC ACQUITY UPLC-H Class 1-11-21-3 1-1 LC-MS/MS WatersACQUITY UPLC BEH HILIC 1.7 µm 2.1 1 mm A.5 B.2 ml/min 5 µl 4 5 11 11.1 13 13.1 2 A 1 1 25 7 7 1 B 99 99 75 3 3 99 99 1-2 MS Tune ESI.5 kv 1L/ hr55 15 1-3 MRM ESI Cone(V) CE(eV) RT TTX + 32> 32 48 26 32 > 162 48 5 12. (1) TTX.5 ng/ml 1 2 32 > 32.21 ng/ml.999 1-1 1-2 32 > 162 1-1 TTX 1-1 TTX (.21 ng/ml) 1-2 TTX1ng/mL.443 ng/ml - 31 -
2.2µg/g.8 ng/ml 32>32 2 TTX ng/ml ng/ml IDL (3σ) IQL (1σ).476.443.133.443 9.3 (2) 2-1 TTX 1 MU/g 2.2 µg/g 1 2 µg/g 2 µg/g 1 3 TTX.5 ng/ml 1 1 2 % 25 ml 1 3,rpm 1 4 5 ml 2 % 1 ml 2mL 2 % 5 ml 5 ml 4 ml.2 µm LC-MS/MS HLB ODS ACNMeOH75151 PRiME HLB AmiconUltra-4 3 TTX µg/g 2 2 PRiME-HLB 12.6 --- PRiME-HLB 95.3 --- PRiME-HLB --- 76.4 HLB 89.7 75.8 ODS 96.8 71.9 88.8 78.4 94.8 69.4-32 -
(3) 1 ng/ml STD1 1 ng/ml STD2 ME STD2 STD111-8 1-71% 4 1 ng/ml 2-2 GCB AC2 2 GCB AC2 5 %5 5 4 1 g/ml 1 g/ml 1 7558 ME % 1 1256-83 2 1313-83 5 1795-76 1 22-71.5 ml ODS GCB GCB 5 ml1 ml AC2 3 ml 2EtOH 14mL 5 TTX 5 GCB AC2 48.7 68.9 ACNMeOH:H 2 O7515:1) 1 ml.2 µm LC-MS/MS 2-2 LC-MS/MS TTX HILIC.21 ng/ml R=.999 TTX 25-33 -
2 ng/ml 1) LC-MS/MS TTX 48 2) LC/MS/MS 19 1-34 -
1-35 -
11/7-11/3 11/3-12/26 12/26-1/19 1/19-2/8 2/8-3/5 (23 ) (26 ) (24 ) (2 ) (25 ) (25) 6.5km (1) 6km (1) 15km (1) 1km 12 2km - 36 -
3 3 2 2 1.. 1cm 2,12.5cm8cm1ml 5ml 2rpm 1min.2m ICS-11μg/cm 2 / - 37 -
3-38 -
- 39 -
- 4 -
196 197 12 2 28 1) H nss-so4 2 nssnon-sea salt 19 2 29 2151-1 37.88 138.99 15m 15m 1.7km 29 4 29 1 3 3 29 11 29 1-41 -
US-33 314cm 2 2 2 2) 1 1 ph EC SO4 2 NO3 Cl NH4 Na K Ca 2 Mg 2 ph EC ph EC 1 ph 29 4 9 4.85 ph 5.6 EC 29 1.45mS/m phec 1 3 EC [ms/m] ph ph EC 5.6 5.2 49 13 4.8 4.4 4. H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 1. 8. 49 13 6. 4. 2.. H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 1 ph EC - 42 -
5 2327 28 29 2(a) 2(b) 2(a) 29 28 5 NH4 NO3 Na Cl Na Cl 4 NO3 nss-so4 2 2(b) NO3 nss-so4 2 1214 16 nss-so4 2 NH4 NO3 nss-so4 2 4555 354 ph [%] 1 8 6 4 2 H29 H28 5 H28 5 49 13 (a) [μeq/l] 7 6 5 4 3 2 1 H29 H28 5 H28 5 Na+ Cl- Mg2+ ss-so42- K+ nss-so42- ss-ca2+ NO3- nss-ca2+ NH4+ H+ nss-ca2+ NH4+ H+ nss-so42- NO3-49 13 (b) 2 R1R2 28 29 R1 R2 3 R1 9-43 -
R1 R2 R1 [%] 4 3 2 1-1 -2-3 -4 H28 H29 1 1 1 1 1 Cations + Anions [μeq/l] R2 [%] 3 2 H28 H29 1-1 -2-3.1 1 1 1 EC [ms/m] 3 R1 R2 EC 5 2327 28 29 4 EC Na nss-ca 2 3 5 29 4 nss-ca 2 NO3 nss-so4 2 NH4 29 9 3) H NO3 29 5 nss-so4 2 H 4) 29 5 5) 1mm 12 1 1 15 5 65mm115mm 29 1134mm Na 1 EC - 44 -
NO3 NH4 5 12 EC [mm] 4 3 2 1 [ms/m] 1 8 6 4 2 25 4 5 6 7 8 9 1 11 12 1 2 3 nss-ca2+ 8 4 5 6 7 8 9 1 11 12 1 2 3 Na+ [μmol/l] 2 15 1 5 [μmol/l] 6 4 2 4 5 6 7 8 9 1 11 12 1 2 3 4 5 6 7 8 9 1 11 12 1 2 3 [μmol/l] 7 6 5 4 3 2 1 [μmol/l] 4 35 3 25 2 15 1 5 NO3-4 5 6 7 8 9 1 11 12 1 2 3 nss-so42-4 5 6 7 8 9 1 11 12 1 2 3 8 NH4+ 7 H+ [μmol/l] 7 6 5 4 3 2 1 [μmol/l] 6 5 4 3 2 1 4 5 6 7 8 9 1 11 12 1 2 3 4 5 6 7 8 9 1 11 12 1 2 3 3 1mm 25 [day] 2 15 1 5 H28 H29 H23H27 H23H27 H23H27 4 5 6 7 8 9 1 11 12 1 2 3 4-45 -
[mm] [ueq/l] 15 13 11 9 7 5 35 3 [ueq/l] [ueq/l] 25 2 15 1 5 35 3 25 2 15 1 5 15 12 9 6 3 [ueq/l] [ueq/l] [ueq/l] 35 3 25 2 15 1 5 6 5 4 3 2 1 5 4 3 2 1 H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 nss-ca2+ Na+ H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 NO3- H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 NH4+ H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 nss-so42- H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 H+ H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 49 13 H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 5 nss-so4 2 NO3 22 NO3 H nss-so4 2 19 4.5 ueq/l 2 25.7 ueq/l Ca 2 NO3 NH4 nss-so4 2 ph ph - 46 -
NH4 6 1 1 I: μeq/l NH : NH μeq/l 6 nss-so4 2 H 23 27 21 24 nss-so4 2 H 18 23 Ca 2 NO3 NH4 H nss-so4 2 15 1 5-5 nss-so42- H+ NO3- nss-ca2+ 15 1 5-5 nss-so42- H+ NO3- nss-ca2+ -1 H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29-1 H18 H19 H2 H21 H22 H23 H24 H25 H26 H27 H28 H29 6 NH4 29 1 ph EC ph EC Na Cl NO3 nss-so4 2 EC Na NO3 nss-so4 2 NH4 H NO3 nss-so4 2 1mm - 47 -
Na NO3 NH4 NO3 NH4 nss-so4 2 NO3 22 NO3 H nss-so4 2 19 2 ph NH4 Ca 2 nss-so4 2 1) 28 4141-47217 https://www.city.niigata.lg.jp/iryo/shoku/syokuei/shokueishisetsu/eisei_ken/eiken_chous a.files/h28nenpo_kenkyuu.pdf 2) 2 21https://www.env.go.jp/air/acidrain/man/wet_deposi/index.html 3) 27 466-72216 4) 3 2 2 http://www.data.jma.go.jp/gmd/env/kosahp/kosa_data_index.php 5) 3 2 1 http://www.data.jma.go.jp/obd/stats/etrn/index.php - 48 -
16 5 14 11 17 21 5 37 22 26 29 2 4 27 29 ASPT 2 2 Shannon 2 26 29 3 ASPT 2 1 29 2 (1) 29 5 15 18 (2) 1 St- 1 St- 2 St- 3 St- 4-49 - St- 1 St- 2 St- 3
St-3 St-3 St-2 St-4 St-1 St-1 St-2 (3) 2 ph (4) 2 Shannon 62 11 ASPT ASPT 1 29 3 18 62 18 71 ASPT ASPT 7.5 6. 7.5 5. 6. 5. - 5 -
ASPT ASPT ASPT ASPT ASPT =() 33 4 Shannon Shannon Weaver1963 () H n N S N nii 3 (1) 2 3 (a)(b) Shannon 4-1 St- 1 3m 2 1 /s 7 3 14ASPT 7.9 181 1 Shannon 2.2-2 St- 2 5m 4 5 /s 3 2 11ASPT 7.1 St- 1 12 11 Shannon 1.9 St- 1-51 - ln n N
-3 St- 3 BOD75 7m 4 9 9 5 3 127 58 St- 1 9ASPT 4. St- 1 St- 2 St- 1 Shannon 2.2 St- 1 St- 2 ASPT Shannon -4 St- 4 25m 5 9 7 5 3 7ASPT 3.9 St- 3 2 87 Shannon 1.6 3-52 -
a - 53 -
b - 54 -
Shannon - 55 -
ph 6.7 7.2 Shannon SS St- 1 St- 2St- 3 27 29 ASPT St- 1 8. St- 2 7.3 St- 3 4.8 St- 4 4.5 2 ASPT Shannon St- 1 St- 3 2 St- 4 1.2 1.6 5 Shannon - 56 -
BOD75% ASPT 2 BOD75 7 ASPT St- 1 St- 3 3 23 24 BOD75% 11 18 St- 4 ASPT St- 1 8 St- 2 1 7 8 St- 3 7m 29 4. St- 4 Shannon 27 1.2 1.6 ASPT 3.9 2 BOD75% ASPT (a) St- 1 (b) St- 2 (c) St- 3 (d) St- 4 2 BOD75% ASPT (2) 6 7 (a)(b) 8 ASPT Shannon -1 St- 1 4m 8 16 /s 26 4 34m - 57 -
9 7 3 ASPT 6.3 ASPT 5.7 Shannon.71-2 St- 2 5m 7 12 /s 7 3 1 9 ASPT 6. ASPT 5.4 Shannon 1.5 St- 1-3 St- 3 8m 7 1 /s 1 2 7 3 2 ASPT 5. 26 28 3 2 ASPT 4 1 ASPT 2 6.2 Shannon 1. 6-58 -
7 (a) (b) - 59 -
8 ASPT Shannon 9 ASPT ASPT ASPT ASPT Shannon 9 3 ph 6.97.4 8 2 ASPT St- 1 5.6 St- 2 5.7-6 -
St- 3 3.3 St- 1 St- 2 ASPT ASPT St- 3 7 3 4 ASPT ASPT ASPT St- 1 St- 2 St- 3 3 St- 3 St- 1 St- 2 St- 3 Shannon St- 2 1.5 9 Shannon - 61 -
BOD75 ASPT 3 BOD75ASPT ASPT St- 1 St- 3 BOD75 St- 1St- 3 1 BOD75ASPT St- 1 St- 2 6 St- 3 28 29-3 ASPT BOD75 ASPT St- 2 St- 3 ASPT St- 1 (a)st- 1 1 (b)st- 2 1 1.5 (c)st- 3 2 2 3 3 ASPT BOD75 4 (1) 27 29 ASPT St- 1 8. St- 2 7.3 St- 3 4.8 St- 4 4.5 2 ASPT 27 29 Shannon St- 1 St- 3 2 St- 4 1..5-62 -
(2) 26 29 ASPT St- 1 5.6St- 2 5.7 2 St- 3 3.3 St- 1 3.St- 2 4.5St- 3 3.3 4 5. 3 Shannon St- 2 1.5 St- 1St- 3 1 BOD75 26 29 4 St- 1 3 1St- 2 2 1St- 3 4 5 (3) 26 29 ASPT St- 1 6.1 St- 2 5.5 St- 3 3.9 (2) ASPT ASPT 26 1 2 ASPT ASPT St- 1 3 St- 1 St- 2 2 ASPT ASPT 5-63 -
25-64 -
- 65 -
- 66 -
- 68 -
- 69 -
- 7 -
- 71 -
1 1 23 1 No.6 No.1 COD a COD 6 1 2 a a COD COD No.6 No.1 1) 16 1 216 3 3 7 4 2 26 COD 29 217 217 4 1 7 1 1) No.6 No.7No.1 No.2 m-3m+1m 1-72 -
No.2 No.3 No.1 No.1 No.3 No.4 No.6 No.7 No.1 A B 1 1) 1 1 No.6 ィ a C O D C O D No.7 No.1 No.2-73 -
217 1 2 7 No.2 8 COD Chl.a Cl 217 7 1 [] 35 3 25 2 15 1 5 No.6 No.7 No.1 No.2 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 + 2 COD [mg/l] 5 4 3 2 1 No.6 No.7 No.1 No.2 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 3 COD Cl [mg/l] 25 2 15 1 5 No.6 No.7 No.1 No.2 25 2 15 1 5 Cl [mg/l] 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 Cl - 74 -
Chl.a [mg/l] 25 2 15 1 5 No.6 No.7 No.1 No.2 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 5 Chl.a T-N [mg/l] 1.2 1..8.6.4.2. No.6 No.7 No.1 No.2 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 6 T-N T-P [mg/l].1.8.6.4.2. No.6 No.7 No.1 No.2 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 7 T-P - 75 -
COD COD 75% A COD75% 2 No.1No.3 No.4 COD No.6 No.1 COD 5. A COD 75% [mg/l] 4. 3. 2. 1.. No.1 No.3 No.4 No.6 No.1 (2mg/L) 2 A COD 75% B COD75% 3 No.7 B COD COD 75% [mg/l] B 5. 4. 3. 2. 1.. No.7 (3mg/L) 3 B COD 75% A COD75% 3 No.1No.2-76 -
No.3 COD 5. A COD 75% [mg/l] 4. 3. 2. 1.. No.1 No.3 No.2 (2mg/L) 4 A COD 75% COD 1 COD 75% 5% 4 1 1 5 7 75% 1 1 54 6 COD 1 5 No.1No.3No.4No.6 No.7 No.1 1 23 No.1No.2 No.3 1 1 No.6 No.1 75% - 77 -
- 78-5 COD 1 1 2 3 4 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.1 1 2 3 4 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.3 1 2 3 4 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.4 1 2 3 4 5 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.6 1 2 3 4 5 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.7 1 2 3 4 5 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.1-1 1 2 3 4 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.1-1 1 2 3 4 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.2-1 1 2 3 4 '89-'98 '9-'99 '91-' '92-'1 '93-'2 '94-'3 '95-'4 '96-'5 '97-'6 '98-'7 '99-'8 '-'9 '1-'1 '2-'11 '3-'12 '4-'13 '5-'14 '6-'15 '7-'16 '8-'17 COD [mg/l] No.3 Ave.3σ Ave.3σ 25% 75% Ave.
Ave.3 No.6 No.1 2mg/L COD 6 COD Cl Cl No.2 Cl No.6 No.1 A Cl No.7B 2 No.6No.1 2 No.7 2 No.2 Cl [mg/l] 15 1 5 1 2 3 4 5 COD [mg/l] 6 Cl [mg/l] 15 1 5 1 2 3 4 5 COD [mg/l] Cl [mg/l] 15 1 5 COD Cl 29 217 1 2 3 4 5 COD [mg/l] COD COD 7 No.6 COD CODD-COD CODP-COD D-COD P-COD COD 4. No.6 No.6 No.6 COD [mg/l] 3. 2. 1.. 4 5 6 8 9 1 4 5 6 8 9 1 4 5 6 8 9 1 D-COD P-COD D-COD P-COD D-COD P-COD 7 No.6 D-COD P-COD 217-79 -
No.6 No.1 COD achl.a 8 Chl.a 9 COD Chl.a Chl.a COD Chl.a 8 Chl.a 2 9 2 No.6No.1 2 No.6No.1 18 18 Chl.a_calc [μg/l] 16 14 12 1 8 6 4 R² =.7581 Chl.a_calc [μg/l] 16 14 12 1 8 6 4 2 2 1 2 3 4 5 COD [mg/l] 1 2 3 4 [] 8 COD Chl.a 9 Chl.a 215 217 215 217 41 No.6 No.1 3 1 No.6 No.1 6 1 2 35 No.6 No.1 3 25 Max. [] 2 15 1 5 Ave. Min. 4 5 6 7 8 9 1 4 5 6 7 8 9 1 1 No.6 No.1 1989 217-8 -
261 45 No.6 No.1 COD Cl 11 4 5 COD COD COD No.2 12 No.6 No.1 No.6No.14-5 No.6No.16-1 2 2 Cl [mg/l] 15 1 5 Cl [mg/l] 15 1 5. 1. 2. 3. 4. 5. COD [mg/l]. 1. 2. 3. 4. 5. COD [mg/l] 11 No.6 No.1 COD Cl 29 217 No.24-5 No.26-1 2 2 Cl [mg/l] 15 1 5 Cl [mg/l] 15 1 5 1 2 3 4 5 COD [mg/l] 1 2 3 4 5 COD [mg/l] 12 No.2 COD Cl 29 217 No.6 No.1 Chl.a 13 COD Chl.a 14 No.2 No.2 1 217 7 8-81 -
Chl.a COD 2 18 No.6No.1 4-5 6-1 2 18 4-5 No.2 6-1 16 16 Chl.a_calc [μg/l] 14 12 1 8 6 Chl.a_calc [μg/l] 14 12 1 8 6 4 4 2 2 1 2 3 4 1 2 3 4 [] [] 13 Chl.a 215 217 No.6No.1 No.2 2 2 18 16 4-5 6-1 18 16 4-5 6-1 Chl.a_calc [μg/l] 14 12 1 8 6 Chl.a_calc [μg/l] 14 12 1 8 6 4 4 2 2 1 2 3 4 5 1 2 3 4 5 COD [mg/l] COD [mg/l] 14 COD Chl.a 215 217 No.6 No.1 COD Chl.a 14 2COD - 82 -
5 4 COD [mg/l] 3 2 1 1 15 2 25 3 [] 1 5.4.8 14.6 Chl.a [μg/l] 14 Chl.a COD No.6 No.1215 217 T-N Cl 15 T-P Cl 16 No.2 T-N T-P No.6 No.1 T-N T-P No.7 No.6 No.1 2 No.6No.1 2 No.7 2 No.2 Cl [mg/l] 15 1 5.2.4.6.8 1 T-N [mg/l] 15 Cl [mg/l] 15 1 5.2.4.6.8 1 T-N [mg/l] Cl [mg/l] 15 1 5 T-N Cl 29 217.2.4.6.8 1 T-N [mg/l] 2 No.6No.1 2 No.7 2 No.2 Cl [mg/l] 15 1 5.2.4.6 T-P [mg/l] Cl [mg/l] 15 1 5.2.4.6 T-P [mg/l] 16 T-P Cl 29 217-83 - Cl [mg/l] 15 1 5.2.4.6 T-P [mg/l]
217 No.6 No.1 2mg/L COD 75% No.1No.2 No.3 COD 75% COD 1 1 23 1 No.6 No.1 A No.7B No.6 No.1 261 COD No.6 No.1 Chl.aCOD COD 7 COD P-COD D-COD COD COD COD 1) 29 2) 1989-216http://www.pref.niigata.lg.jp/ kankyotaisaku/1254822231356.html218.1.5 3) 29 12-17 (217) 4) 28 41 65-75(217) - 84 -
[ms/m] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1 52 32 22 94 15 2 No.6 37 35 39 36 36 37 46 47 47 46 46 48 3 27 37 29 21 28 No.7 38 37 45 41 36 42 46 46 49 46 46 47 23 26 24 18 26 46 No.1 35 29 31 41 38 47 47 46 48 47 47 47 48 46 49 42 47 47 No.2 48 46 49 47 47 47 48 47 49 48 48 48 5 4 7 7 7 8 8 8 12 13 13 18 8 8 13 12 14 15 [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1 157 964 769 32 51 694 No.6 138 132 147 134 137 138 181 181 181 184 183 187 18 979 139 14 751 1 No.7 141 136 173 157 138 163 182 176 19 184 181 185 799 928 847 69 935 182 No.1 13 15 112 155 146 183 185 177 186 185 185 186 188 179 192 163 189 185 No.2 189 179 192 184 189 185 191 184 194 19 189 187 7 2 4 3 4 6 7 6 1 12 1 22 3 7 1 9 1 11 a [μg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1.2.6 15.5 3.6 4. No.6 1.3 1.1 4.9 4.6 6.4 3.6 <.1.6 1.1.9 1.7 1.1 2. 1.1 6.1 5.1 4.1 1.1 No.7 2. 1.1 2.5 7.3 2.8 3. 1.3 1.2.4 3.3 2.4 2.8 2.4 1.4 5.1 4.6 7.5 3. No.1 1.5 1.1 6.5 3.3 5.7 2.8.6.9 1.2 1.1 1.1 1.7.2.9.3 12.4 1.6 No.2.2.8.4 2..4 1.6.2.3.4.4.3.4.4.6 3..6 1.5 6..9 2.8 18 4.6 4.9 4.1 1.5 2.2 17 2.2 4.6 3. < - 85 -
COD [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1 1.6 1.4 2.9 3.8 2.5 2. No.6 1.6 1.9 2.2 2.3 2.8 2.2 1.4 1.5 1.8 2. 1.5 1.5 2.1 1.9 2.1 2.5 2.1 2. No.7 1.7 1.8 1.9 2.4 1.8 2.2 1.7 1.5 1.9 2.1 1.3 1.8 1.8 1.8 2.3 3. 2.4 2.3 No.1 1.7 1.7 2.2 2.1 2.5 2.3 1.4 1.4 1.6 1.8 1.4 1.8 1.5 1.7 1.5 3.2 1.3 1.5 No.2 1.4 1.6 1.5 1.9 1.5 1.5 1.2 1.6 1.3 1.5 1.1 1.4 1.7 1.6 2. 3. 1.6 1.8 1.9 2.2 3.2 3. 2.2 2.3 1.9 2.2 3. 2.6 2.3 2.2 [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1.35.33.47.36.57.22 No.6.18.38.27.32.31.25.24.2.13.12.14.8.45.52.29.51.51.63 No.7.38.33.28.27.31.32.23.28.12.23.14.13.39.27.31.43.39.16 No.1.26.36.3.21.21.15.18.11.1.11.22.19.14.12.7.25.7.13 No.2.15.2.7.14.8.11.11.9.9.8.8.11.34.27.24.44.29.28.84.66.81.86.79.99.81.72.82.87.92 1. [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1.21.16.35.36.43.15 No.6.18.19.24.27.24.18.13.22.14.13.17.12.35.44.36.43.37.43 No.7.29.25.22.25.24.33.23.13.17.17.14.17.19.26.28.36.22.15 No.1.16.2.1.17.19.13.16.12.9.11.16.12.18.19.1.21.14.1 No.2.11.1.9.11.9.1.14.13.1.1.8.9.22.18.15.42.18.19.5.59.73.73.34.79.54.59.75.7.39.84-86 -
COD [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1.9 1. 1.9 2.3 1.4 1.4 No.6 1.4 1.4 1.7 1.7 1.2 1.4 1.4 1.5 1.3 1.5 1.1 1.2 --- --- --- --- --- --- No.7 --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- No.1 --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- No.2 --- --- --- --- --- --- --- --- --- --- --- --- 1.2 1.1 1.5 2.3 1.6 1.5 1.7 1.5 2. 2.1 2. 1.7 1.4 1.4 1.9 2. 1.3 1.6 --- [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1.28.23.34.32.46.21 No.6.13.19.26.25.18.19.2.8.12.17.13.12.4.42.31.45.49.57 No.7.26.24.23.25.26.22.14.11.11.14.12.1 --- --- --- --- --- --- No.1 --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- No.2 --- --- --- --- --- --- --- --- --- --- --- ---.31.27.24.37.3.31.72.55.73.77.7.93.67.58.75.79.78 1. --- [mg/l] '17/4/25 '17/5/9 '17/6/19 '17/8/1 '17/9/5 '17/1/1.9.7.15.7.8.7 No.6.7.9.11.9.5.9.11.9.14.9.8.9.22.15.7.15.21.15 No.7.12.11.9.11.11.11.15.14.9.14.7.14 --- --- --- --- --- --- No.1 --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- --- No.2 --- --- --- --- --- --- --- --- --- --- --- ---.1.5.8.17.22.9.22.15.3.33.19.51.2.15.4.38.23.56 --- - 87 -
27 27 28 29 2.25mg/kg 1-88 -
23mg/kg 4311mg/kg 2.25mg/kg 5.5.4 1 2.25mg/kg.45.5-89 -
13 3-9 -
QuECheERS 2 2 2 2.5ppm 8299 15 8 29 215-91 -
2 5 1 DNPH - 92 -
31 1 95223 2151 1 TEL 25(231)1231 FAX 25(23)5818 e-maile ise i.r c @ c i t y. n iigata.lg.jp - 4 -