pp. -O Mo Mo Mo EC EC xanthine oxidoreductase XOR EC AO EC Structure and mechanism of molybdenum hydroxylase Ken Okamoto and Takeshi Nishino Department of Biochemistry, Nippon Medical School, Sendagi, Bunkyo- Ku, Tokyo, Japan A A XOR AO B B DMSO
A B XOR XOR B XOR Fe- SFAD Beinert ESR electron spin resonance Bray ESR XOR XOR XOR P XOR AO XOR NAD xanthine dehydrogenase XDH xanthine oxidase XO XO NAD O XO
XOR XOR XOR FAD nm nm Mössbauer circular dichroism ESR ESR Mo rapid slow rapid proton hyperfine splitting type type Rhodobacter capsulatus XOR -S- S O OH S O Desulfovibrio. gigas AO S - -pyridin- -yl- H- triazol- -yl pyridine- -carbonitrile FYX- Mo S Mo-SH magnetic circular dichroism X-ray absorption spectroscopy XOR A B O XOR XOR XOR XDH XO XOR kda A Fe- S FAD B Fe S I Fe S II Fe S II Fe S I Fe S II FAD NAD Fe S I FAD Fe S II FAD C Fe S II Fe S II electron sink FAD
A B C XOR TEI- Y- FYX- Ki M FYX- Arg Thr Glu C Phe Phe edge-on interaction Glu Glu Glu Arg XOR Glu Arg Aspergillus nidulans, R. capsulatus XDH XOR Mo O -OH S -S- O
O Mo O Mo-OH DMSO O Howes Mo-C D. gigas AO Mo-O Mo-O-C H Mo S Mo S Mo-OH O ENDOR electron nuclear double resonance FYX-A Mo -OH-FYX- FYX- FYX- C B Mo-C Mo-O O-C B Mo-OH Glu Glu Mo-OH Mo-O- Mo-O-C Glu XOR AO R. capsulatus Glu Glu Glu Glu A FYX- -OH-FYX- B XOR-FYX- fo-fc map σ C XOR-FYX-
FYX- N C Mo-O Mo S Mo S Mo-SH Mo -OH FYX- XOR A C N Mo XOR N B FYX- N -OH Glu Glu Arg B AO Glu Arg Truglio R. capsulatus XOR XOR Glu Arg Phe Phe Glu C Glu ArgGlu Glu A B XOR- fo-fc map σ
Glu ph Glu kcat Arg Arg EV kcat A. nidulans Arg -hydroxypurine -hydroxypurine A B C AO
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