PathScan Antibody Array Kits

athscan Antibody Array Kits

athscan Antibody Array Kits athscan Antibody Array CST athscan Antibody Array Nicole, roduct Scientist has beenen at CST for over years. 0 µl (0.-0. mg/ml) athscan RTK Signaling Antibody Array Kits Chemiluminescent Readout #98 Fluorescent Readout #99 9 ositive Control 9 9 9 9 9 9 0 8 8 0 0 0 8 8 8 8 Negative Control Target map of the athscan RTK Signaling Antibody Array Kit #99 0 0 8 8 9 9 8 Intracellular Signaling Akt Signaling EGFR Signaling 8 Immunology 9 Thr/Th/Th Cytokine 0 Cancer henotype FAQs Receptor Tyrosine Kinases Target Site Family + ositive Control N/A Negative Control N/A EGFR/ErbB pan-tyr EGFR HER/ErbB pan-tyr EGFR HER/ErbB pan-tyr EGFR FGFR pan-tyr FGFR FGFR pan-tyr FGFR FGFR pan-tyr FGFR InsR pan-tyr Insulin R 8 IGF-IR pan-tyr Insulin R 9 TrkA/NTRK pan-tyr NGFR 0 TrkB/NTRK pan-tyr NGFR Met/HGFR pan-tyr HGFR Ron/MSTR pan-tyr HGFR Ret pan-tyr Ret ALK pan-tyr LTK DGFR pan-tyr DGFR c-kit/scfr pan-tyr DGFR FLT/Flk pan-tyr DGFR 8 M-CSFR/CSF-R pan-tyr DGFR 9 EphA pan-tyr EphR 0 EphA pan-tyr EphR EphA pan-tyr EphR EphB pan-tyr EphR EphB pan-tyr EphR EphB pan-tyr EphR Tyro-/Dtk pan-tyr Axl Axl pan-tyr Axl Tie/TEK pan-tyr Tie 8 VEGFR/KDR pan-tyr VEGFR Signaling Nodes Target Site Family 9 Akt/KB/Rac Thr08 Akt 0 Akt/KB/Rac Ser Akt p/ MAK (ERK/) Thr0/Tyr0 MAK S Ribosomal rotein Ser/ RSK c-abl pan-tyr Abl IRS- pan-tyr IRS Zap-0 pan-tyr Zap-0 Src pan-tyr Src Lck pan-tyr Src 8 Stat Tyr0 Stat 9 Stat Tyr0 Stat

One Experiment, Numerous Data oints 実験例 athscan Stress and Apoptosis Signaling Antibody Arrayキットを用いた ストレスに対する細胞応答の定量化 athscan Stress and Apoptosis Signaling Antibody Array Kits Chemiluminescent Readout #8 Fluorescent Readout #9 アレイあたりの標的分子数 9 キットあたりのデータポイント数 08 処理 下流への影響を特定するために 様々 な細胞種にStaurosporine EGFまたはUV 照射の処理を行うか または未処理の ままにしました メモ この実験では七つの実験条件のみ示していま すが キットあたり最大種類のサンプル を試験することが可能です athscan Stress and Apoptosis Signaling Antibody Arrayキットは 回の実験で様々な濃度や細胞株 タイムポ イントを組み込む 充実した実験計画を実現 します 解析 #9 athscan Stress and Apoptosis Signaling Antibody Array Kit (Fluorescent Readout) を用いて 下流のシグナル伝 達事象を観察しました 蛍光の読み取りには LI-COR Biosciences Odyssey CLxイメージングシステムを使用 しました 9 0 8 8 9 9 0 0 8 8 9 9 8 0 0 Target ositive Control Negative Control p/ MAK (Erk/) Akt Bad HS Smad 8 p 9 p8 MAK 0 SAK/JNK AR Caspase- Caspase- IκBα Chk Chk IκBα 8 eifα 9 TAK 0 Survivin α-tubulin Site N/A N/A Thr0/Tyr0 Ser Ser Ser8 Ser/ Ser Thr80/Tyr8 Thr8/Tyr8 Asp Asp Asp98 Total Ser Thr8 Ser/ Ser Ser Total Total Detection Antibody 定量 Target rotein 蛍光強 度は LI-COR Image Studio v.0 アレイ解析ソフトウェアを使用して定 量化しました 蛍光強度の生データを MultiExperiment Viewerソフトウェアで処 理してヒートマップ解析を行いました K- HT-9 + UV HT-9 A + EGF HeLa HeLa + Staurosporine A Capture Antibody Cleaved Caspase- (Asp) Cleaved AR (Asp) Cleaved Caspase- (Asp98) IκBα (Ser/) Smad (Ser/) eifα (Ser) TAK (Ser) Survivin (Total) Chk (Ser) Chk (Thr8) Akt (Ser) p (Ser) p8 MAK (Thr80/Tyr8) HS (Ser8) SAK/JNK (Thr8/Tyr8) IκBα (Total) p/ MAK (Erk/) (Thr0/Tyr0) Bad (Ser) α-tubulin (Total) 結果 ストレスあるいはアポトーシスの特 性を示す 様々な分子的特徴が認 められました (黄色) 例 Staurosporineは HeLa細胞のAR Caspase-およ びCaspase-の開裂を誘導しました HT-9細胞では UV照射によりp p8 HS SAK/JNKなどの一連のストレス誘導性標的分子の リン酸化が引き起こされました Low Fluorescence High Fluorescence www.cstj.co.jp/pathscanarrays

Intracellular Signaling athscan Intracellular Signaling Array Kits Intracellular Signaling Array Target Site ositive Control N/A Negative Control N/A Erk/ Thr0/Tyr0 Stat Tyr0 Stat Tyr0 Akt Thr08 Akt Ser 8 AMKα Thr 9 S Ribosomal rotein Ser/ 0 mtor Ser8 HS Ser8 Bad Ser p0 S Kinase Thr89 RAS0 Thr p Ser p8 MAK Thr80/Tyr8 SAK/JNK Thr8/Tyr8 8 AR Asp 9 Caspase- Asp 0 GSK-β Ser9 Slide-based Arrays Chemiluminescent Readout # Fluorescent Readout # 8 Target Map A Fluorescent Images of Arrays 8 8 9 9 0 0 8 0 0 8 9 9 9 0 8 B 000 0000 HT-9 SAK/JNK (Thr8/Tyr8) HT-9 HT-9 + UV HS (Ser8) p8 (Thr80/Tyr8) p (Ser) SAK/JNK (Thr8/Tyr8) p (Ser) Quantified Fluorescence HT-9 + UV p8 (Thr80/Tyr8) HT-9 UV # athscan Intracellular Signaling Array Kit (A) LI-COR Biosciences Odyssey CLx (B) (RFU) LI-COR Image Studio v.0 U V p p8 SAK/JNK HS RFU 000 000 8000 000 0 8 9 0 8 9 0 For Research Use Only. Not For Use in Diagnostic rocedures.

athscan Intracellular Signaling Membrane Array Kit Chemiluminescent Readout # 8 Target Map Chemiluminescent Images of Arrays 8 8 9 9 0 0 8 8 9 9 0 0 MCF Erk/ (Thr0/Tyr0) Akt (Thr08) S Ribosomal rotein (Ser/) GSK-β (Ser9) MCF + higf- Akt (Ser) RAS0 (Thr) p0 S Kinase (Thr89) athscan Intracellular Signaling Array Nitrocellulose Membrane mtor athscan Growth factors, insulin, etc. Intracellular Signaling Array DETOR Thr RR GβL mtor RAS0 SIN Rictor Ser Akt Ser8 DETOR mtor IK Ser Ser Ser99 Tyr Wnt TSC Signaling Thr08 TSC Ser8 GβL Rheb AMK GSK- mtor MCF #89 Human Insulin-like Growth Factor (higf-i) (00 ng/ml) 0 # athscan Intracellular Signaling Array Kit (Chemiluminescent Readout) Rapamycin Raptor Ser9 Thr89 p0 SK Ser Ser08 elfg elfa elfe LKB higf-i Akt mtor p0 S Kinase mtor Ser Ser0 Thr E-B Ser Ser S elfb Ser Thr Thr0 Ser DCD Ser Translation and Cell Growth Target present in athscan Intracellular Signaling Antibody Array Kits. www.cstj.co.jp/pathscanarrays

Akt Signaling athscan Akt Signaling Antibody Array Kits Chemiluminescent Readout #9 Fluorescent Readout #900 Target Map + + 8 8 9 9 0 0 + 9 0 8 Target Site + ositive Control N/A Negative Control N/A Akt Thr08 Akt Ser S Ribosomal rotein Ser/ AMKα Thr RAS0 Thr mtor Ser8 GSK-α Ser 8 GSK-β Ser9 9 p0 S Kinase Thr89 0 p0 S Kinase Thr/Ser Bad Ser RSK Ser80 TEN Ser80 DK Ser Erk/ Thr0/Tyr0 E-B Thr/ Target Map: Using athscan Akt Signaling Antibody Array Kit (Fluorescent Readout) #900. A Fluorescent Images of Arrays B Quantified Fluorescence MCF 9 MCF + higf-i 0 0 MCF MCF + higf-i RFU (x0 ) Akt MCF 0 8% #89 Human Insulinlike Growth Factor (higf-i) (00 ng/ml) 0 #900 athscan Akt Signaling Antibody Array Kit (Fluo rescent Readout) 0 8 9 0 (A) LI-COR Biosciences Odyssey CLx (B) (RFU) LI-COR Image Studio v.0 For Research Use Only. Not For Use in Diagnostic rocedures.

EGFR Signaling athscan EGFR Signaling Antibody Array Kits Chemiluminescent Readout # Fluorescent Readout #8 9 8 8 Target Map Sub Array A (Array -8) 8 9 9 0 0 A B 9 0 8 8 8 9 9 0 0 Sub Array B (Array 9-) Target Site ositive Control N/A Negative Control N/A EGFR Total EGFR Thr9 EGFR Tyr8 EGFR Tyr998 EGFR Tyr08 8 EGFR - L88R Mutant Total 9 EGFR - E-A0 Deletion Total 0 MEK Total MEK Total MEK/ Ser MEK/ Ser/ Stat Tyr0 HER/ErbB Total HER/ErbB Tyr9 HER/ErbB Tyr/ 8 Met Total 9 Met Tyr9 0 Met Tyr/ LCγ Total LCγ Ser8 Akt Thr08 Akt Ser Erk/ Thr0/Tyr0 AR Asp Gefitinib EGFR 9 E- Chemiluminescent Images of Arrays Sub-Array A Sub-Array B A0 L8R HCC8 H # athscan EGFR Signaling Antibody Array Kit HCC8 HER (Tyr9) EGFR E-A0 Deletion Met (Total) EGFR L88R Mutant Met (Tyr/) Met (Tyr9) HCC8 EGFR 9 E-A0 H EGFR L8R H LCγ (Tyr8) Akt (Ser) www.cstj.co.jp/pathscanarrays

Immunology athscan Immune Cell Signaling Antibody Array Kits Chemiluminescent Readout #9 Fluorescent Readout #88 9 08 8 8 9 9 0 0 8 8 9 9 0 0 Target Map Target Site ositive Control N/A Negative Control N/A 9 p/ MAK (Erk/) Thr0/Tyr0 0 8 Akt Ser p8 MAK Thr80/Tyr8 SAK/JNK Thr8/Tyr8 Caspase- Asp98 8 IκBα Total 9 IκBα Ser/ 0 TAK Ser Stat Tyr0 Stat Ser Stat Tyr0 Stat Ser Stata Tyr9 Stat Tyr Lck Tyr 8 Syk Tyr 9 Zap-0 Tyr9 0 Rig-I Total IRF- Total Chemiluminescent Images of Arrays Ramos Ramos + IgM Syk (Tyr) Ramos Anti-human IgM ( µg/ml) Jurkat (0 mm) #9 athscan Immune Cell Signaling Array Kit Jurkat Jurkat + H O Lck (Tyr) Zap-0 (Tyr9) Lck (Tyr) Zap-0 (Tyr9) Ramos IgM Syk Tyr Jurkat Lck Tyr Zap-0 Tyr9 8 For Research Use Only. Not For Use in Diagnostic rocedures.

Thr/Th/Th Cytokine athscan Th/Th/Th Cytokine Antibody Array Kits Chemiluminescent Readout #0 Fluorescent Readout # 8 Target Map 9 0 8 8 8 9 9 0 0 Target rotein ositive Control Negative Control IL- IL- IL- IL- IL-8 8 IL-0 9 IL- p0 0 IL- IL-A TNF-α GM-CSF IFN-γ A Fluorescent Images of Arrays B Quantified Fluorescent IL- BMC IL- IL-8 BMC + IFN-γ + LS IL-8 00 BMC BMC + IFN-γ + LS TNF-α 00 IFN-γ RFU (x0 ) 00 (BMC) #890 Human Interferon-γ (hifn-γ) LS ( µg/ml) # athscan Th/Th/Th Cytokine Antibody Array Kit (A) LI-COR Biosciences Odyssey (B) (RFU) LI-COR Image Studio v.0 00 0 8 9 0 BMC IFN-γ LSIL- IL-8 TNF-α IFN-γ www.cstj.co.jp/pathscanarrays 9

Cancer henotype athscan Cancer henotype Antibody Array Kits Chemiluminescent Readout #8 Fluorescent Readout #8 Target Site Modification 9 08 ositive Control N/A N/A Negative Control N/A N/A Target Map 8 8 9 9 0 0 Vimentin 8 8 9 9 0 0 9 0 8 Chemiluminescent Images of Arrays HeLa CD A- EpCAM CD (ECAM-) Total N/A EpCAM Total N/A Vimentin Total N/A CD Total N/A CD Total N/A 8 CNA Total N/A 9 Ki- Total N/A 0 p Kip Total N/A E-Cadherin Total N/A N-Cadherin Total N/A VE-Cadherin Total N/A MUC Total N/A Rb Ser80/8 hosphorylation HIF-α Total N/A Survivin Total N/A 8 p Total N/A 9 HER/ErbB Total N/A 0 Met Total N/A EGF Receptor Total N/A HeLa A- HCC8 Calu- 90% #8 athscan Cancer heno type Antibody Array Kit N-Cadherin E-Cadherin VE-Cadherin p EGFR MUC HCC8 Vimentin EGFR p Calu- CD HER/ErbB athscan Antibody Array Kits ( ) #9S #900S #8S #8S #S #8S #88S #9S #S athscan Akt Signaling Antibody Array Kit athscan Akt Signaling Antibody Array Kit athscan Cancer henotype Antibody Array Kit athscan Cancer henotype Antibody Array Kit athscan EGFR Signaling Antibody Array Kit athscan EGFR Signaling Antibody Array Kit athscan Immune Cell Signaling Antibody Array Kit athscan Immune Cell Signaling Antibody Array Kit athscan Intracellular Signaling Membrane Array Kit Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit 99,000 - C ( ) #S #S #99S #98S #8S #9S #S #0S athscan Intracellular Signaling Array Kit athscan Intracellular Signaling Array Kit athscan RTK Signaling Antibody Array Kit athscan RTK Signaling Antibody Array Kit athscan Stress and Apoptosis Signaling Antibody Array Kit athscan Stress and Apoptosis Signaling Antibody Array Kit athscan Th/Th/Th Cytokine Antibody Array Kit athscan Th/Th/Th Cytokine Antibody Array Kit Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Kit,000 - C Visit our website for the latest additions to our growing family of athscan Antibody Array Kits. 0 For Research Use Only. Not For Use in Diagnostic rocedures.

FAQs for athscan Antibody Array Kits Q Q CST LI-COR Odyssey Typhoon FLA 9000 INNOSYS InnoScan 0-IR 80 nm 00 nm DyLight 80 Alexa Fluor 9 nm nm Q LI-COR Odyssey CLx Setup Grid Array Controls Resolution, ; Quality, High; Focus offset, 0.0 mm Channels Check the 00 box, deselect the 800 box and deselect Auto Channel Intensities setting Intensity L Q / MT Q mm x mm x Q #980 #08 Q 0. mg/ml 0. mg/ml 0.-.0 mg/ml Q Q CST Q Q Q Troy, roduct Scientist, has been with CST since 00. CST CST www.cstj.co.jp/support 0 Cell Signaling Technology, Inc. Cell Signaling Technology, CST and athscan are trademarks of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. rinted on FSC certifi ed paper using vegetable inks and processed chlorine free. Support info@cstj.co.jp FO

Cell Signaling Technology (CST) 00 CST CST www.cstj.co.jp CST Antibody erformance Guarantee: To learn more, please visit: www.cellsignal.com/abguarantee. COVER IMAGE: Cellular Landscape: Vesicle Traffi cking www.cstj.co.jp/vesicletraffi cking. BROARRYNONE00JN_00 For Research Use Only. Not For Use in Diagnostic rocedures. 0-00 --00 Tel 0-9-0 / Support info@cstj.co.jp www.cstj.co.jp