Contents PCR...3 PCR TaqMan...6 TaqMan / PCR / PCR...8 PCR RNA...10 Cells-to-CT Kit...12 Cell-to-CT Kit RT-PCR TaqMa

real-time PCR PCR 2015 7

Contents PCR...3 PCR...3...4 TaqMan...6 TaqMan / PCR / PCR...8 PCR...9...10 RNA...10 Cells-to-CT Kit...12 Cell-to-CT Kit...14 1 RT-PCR...15...16 TaqMan Fast...18 TaqMan...19 SYBR Green...23 TaqMan...24 TaqMan 96...25 TaqMan 384...28 TaqMan...29 RNAi...32 sirna...33 RNA sirna mirna...36 microrna...37 microrna...38 microrna...39 microrna...40 Non-coding RNA...42 microrna...43 SNP...44 SNP...45 SNP...46...48...49 High Resolution Melt HRM...50...52...55...56...59 TaqMan...60...61 INDEX...63 INDEX...69

PCR PCR TaqMan SYBR Green RNA PureLink RNA Kit TRIzol Reagent TRIzol Plus RNA Purification System TaqMan Cells-to-CT Kit Ambion Single Cell-to-CT Kit 10 10 10 13 14 MagMax RNAqueous Kit SYBR Green Cells-to-CT Kit 11 11 13 Super Script VILO 16 High Capacity RNA-to-cDNA Kit 17 1 RT-PCR TaqMan RNA-to-CT 1-Step Kit TaqMan Fast Virus 1-Step Master Mix 15 21 Power SYBR Green RNA-to-CT 1-Step Kit 15 PCR TaqMan Fast Advance Master Mix TaqMan Fast Universal PCR Master Mix TaqMan Universal Master Mix II TaqMan Gene Expression Master Mix TaqMan Multiplex Master Mix 18 18 19 20 22 Power SYBR Green PCR Master Mix Fast SYBR Green Master Mix SYBR Select Master Mix 23 23 23 TaqMan TaqMan Gene Expression Assays TaqMan Array Plate TaqMan Array Gene Signature 96-well Plate TaqMan Gene Signature Arrays TaqMan Custom Arrays 24 25 26 28 28 RNAi sirna Silencer Select sirna TaqMan sirna Assays Ambion In Vivo sirna 33 35 35 Lipofectamie RNAiMAX Invivofectamine 3.0 Reagent 36 36 microrna mirvana mirna Isolation Kit mirvana PARIS Kit TaqMan mirna ABC Purification Kit 38 38 38 microrna TaqMan MicroRNA RT Kit 39 Megaplex Primer Pools 39 PCR TaqMan Universal Master Mix II 19 TaqMan TaqMan MicroRNA Assays TaqMan Array MicroRNA Card TaqMan Pri-miRNA Assays TaqMan Non-coding RNA Assays 40 41 42 42 microrna microrna mimic & inhibitor mirvana mirna mimic & inhibitors 43 Lipofectamine RNAiMAX Invivofectamine 3.0 Reagent 36 36 SNP TaqMan Genotyping Master Mix 46 PCR TaqMan GTXpress Master Mix 46 TaqMan TaqMan SNP Genotyping Assays 45 1 SNP TaqMan Sample-to-SNP Kit 47 TaqMan TaqMan Mutation Detection Assays 48 TaqMan TaqMan Copy Number Assays 49 HRM PCR MeltDoctor HRM 50 TaqMan TaqMan Protein Expression Core Reagents Kit with Master Mix TaqMan Protein Assay Kit TaqMan Protein Assay Open Kit 53 53 53 Protein Thermal Shift Dye Kit 55 Protein Thermal Shift Starter Kit 55 3

PCR Applied Biosystems TaqMan Applied Biosystems SYBR Green PCR TaqMan 5'- TaqMan TaqMan PCR TaqMan PCR 5 - TaqMan PCR 5'- TaqMan TaqMan PCR DNA 5'-3' TaqMan 1.Polymerization TaqMan R Q 5' 3' 130 24 TaqMan Gene Expression Assays 2 2 2.Strand displacement TaqMan 3.Cleavage DNA 5'- 3' TaqMan 5' 4.Polymerization completed PCR 1. TaqMan 4

SYBR Green SYBR Green SYBR Green 2 DNA SYBR Green I PCR 2 DNA PCR SYBR Green I PCR Tm PCR 2 DNA SYBR Green I Dye DNA SYBR Green AmpliTaq Gold DNA Polymerase 1.Reaction setup SYBR Green I Dye 2 DNA 2.Denaturation DNA SYBR Green I Dye DNA 3.Polymerization PCR 4.Polymerization completed SYBR Green I Dye 2 DNA PCR 2. SYBR Green 1. TaqMan SYBR Green TaqMan SYBR Green TaqMan 2 DNA SYBR Green I 3 PCR 2 TaqMan 2 PCR 2 1 2 PCR TaqMan Gene Expression Assays 130 1 3 2 PCR 5

TaqMan TaqMan TaqMan microrna mirna Small RNA SNP TaqMan TaqMan SNP c TaqMan PCR TaqMan Gene Expression Assay 23 130 / FAM VIC 4 c 2,700 TaqMan Drug Metabolism Genotyping c Assay PharmaADME DME mirna non-coding RNA mirna non-codingrna ncrna mirna pri-mirna small RNA mirna ncrna TaqMan Protein Assay c c SNP TaqMan SNP 450 TaqMan SNP Genotyping Assay Custom TaqMan SNP Genotyping Assay TaqMan Copy Number Assay 160 18 Custom Plus TaqMan Copy Number Assays Custom TaqMan Copy Number Assays 2. TaqMan TaqMan Gene 1 TaqMan MGB 24 Expression Assays cdna Custom TaqMan Gene TaqMan Gene Expression Assays 24 Expression Assays TaqMan Array Plates TaqMan Gene Expression Assays 96 5 Fast 25 TaqMan Array Gene Signature 96-Well Plate TaqMan Gene Signature Arrays TaqMan Custom Arrays TaqMan Array Human Endogenous Control Plate Custom Plus TaqMan RNA Assays 96 TaqMan Gene Expression Assays 384 TaqMan 384 12 384 1 8 TaqMan 32 TaqMan Gene Expression Assays 26-27 28 28 29 24 6

mirna non-coding RNA Megaplex RT Primer Pools for TaqMan MicroRNA Assays TaqMan MicroRNA Assays TaqMan Array MicroRNA Card mirna cdna Multiplex RT 1 mirna TaqMan mirna mirna TaqMan MicroRNA Assays 384 TaqMan 8 TaqMan Non-coding RNA Assays TaqMan Non-coding RNA Assays non-coding 42 TaqMan Pri-miRNA Assays mirna primary mirna pri-mirna 42 SNP TaqMan Pre-Designed SNP Genotyping Assays 450 SNP SNP 2 TaqMan PCR 45 TaqMan Drug Metabolism Genotyping Assays TaqMan Copy Number Assays 221 2,700 Genotyping TaqMan CNV 160 18 TaqMan CNVs TaqMan Copy Number Assays TaqMan Copy Number Reference Assays PCR 3 4 39 40 41 45 49 Custom TaqMan Copy Number Assays & Custom Plus TaqMan Copy Number Assays TaqMan Copy Number Reference Assays TaqMan Mutation Detection Assays TaqMan Copy Number Assays CNVs TaqMan Copy Number Reference Assays TaqMan Copy Number Assays Custom TaqMan Copy Number Assays TaqMan Competitive Allele-Specific TaqMan PCR castpcr MGB 49 49 48 TaqMan Protein Assay Open Kit / TaqMan Protein Assays Core Reagents Kit with Master Mix TaqMan Protein Assay 52-53 2 TaqMan 5 TaqMan Custom Plating Service TaqMan Gene Expression Assays TaqMan MicroRNA Assays 96 384 / / 96 Standard 96 Fast 384 TaqMan Gene Expression Assays Inventoried, Made-to-Order, and Custom 24 TaqMan MicroRNA Assays Inventoried, Made-to-Order, and Custom 40 TaqMan Non-coding RNA Assays Inventoried, Made-to-Order 42 TaqMan SNP Genotyping Assays Predesigned Custom and DME 45 TaqMan Copy Number Assays Predesigned Custom Plus or Custom 49 7

TaqMan TaqMan シングルチューブ 96 もしくは 384 ウェルプレート 384 ウェル TaqMan Array カード OpenArray プレート 1 アッセイより購入可能フレキシブルどんなタイプのリアルタイム PCR 装置にも対応中小規模のプロジェクトに最適反応セットアップの簡便さと柔軟性の適度なバランス 96 および 384 ウェルタイプのすべての装置に対応 1 反応当たりのコストが低い中規模から大規模なプロジェクトに最適 Quant Studio 12K Flex Quant Studio 7 もしくは 7900HT リアルタイム PCR システムの TaqMan Array システムに対応 1 反応当たりのコストを最小に抑え大規模プロジェクトに最適究極のスループット Applied Biosystems Quant Studio 12K Flex OpenArray システムに対応凍結乾燥した混合済みアッセイおよびマスターミックスのフォーマットが利用可能 PCR / PCR PCR StepOne PCR 46 3 Fast StepOnePlus QuantStudio 3 96 4 Fast 30 3 VeriFlex Block QuantStudio 7 New PCR 6 21 4 (96 96 Fast 384 TaqManR Array ) QuantStudio 12K Flex 96 96 Fast 384 TaqMan Array OpenArray 5 1 1 12,000 OptiFlex LED 21 microrna PCR QuantStudio 3D PCR 20,000 8

PCR PCR PCR PCR PCR QuantStudio 3D PCR PCR 1% Custom TaqMan SNP Genotyping Assays EGFR, KRAS, BRAF, PIK3CA, JAK2 TaqMan QuantStudio 3D PCR PCR PCR PCR DNA DNA 3. Custom TaqMan SNP Genotyping Assays KRAS G12V QuantStudio 3D PCR DNA 10%(A) 1%(B) 0.1%(C) D PCR 10.384%(A) 1.393%(B) 0.142%(C) PCR 1 1 DNA PCR PCR PCR PCR 1 / PCR XX / µl QuantStudio 3D PCR PCR QuantStudio 3D PCR ProFlex PCR 1 QS3D-PF 6,400,000 QuantStudio PCR ProFlex PCR QuantStudio 3D ChipLoader 2 QuantStudio 3D PCR Chips v2 12 A26316 11,880 QuantStudio 3D PCR Master Mix v2, 1.5 ml 1.5 ml A26358 17,000 QuantStudio 3D PCR Master Mix v2, 5.0 ml 5.0 ml A26359 48,000 Custom TaqMan SNP Genotyping Assays Custom TaqMan SNP Genotyping Assays for Rare Mutation Analysis 12 4383547 11,500 20x, Small scale Custom TaqMan SNP Genotyping Assays for Rare Mutation Analysis 40x, Medium scale 450 4332077 65,900 TaqMan Gene Expression Assays 9

RNA RNA PureLink RNA Kit Invitrogen PureLink RNA Mini Kit RNA RNA 1,000µg 100µg DNase Set DNA RNA(µg) 1,000 900 800 700 600 500 400 300 200 100 0 PureLink RNA Mini Kit Competitor s midi kit Competitor s mini kit 25 100 200 Tissue(mg) 4. RNA PureLink RNA Mini Kit RNA PureLink RNA Mini Kit PureLink RNA Mini Kit 10 12183020 10,400 50 12183018A 32,100 250 12183025 134,600 PureLink RNA Micro Kit 50 12183016 44,900 PureLink Homogenizer 50 12183026 11,600 PureLink DNase Set 50 12185010 13,000 RNA TRIzol Reagent Invitrogen TRIzol Reagent DNA RNA TRIzol LS Reagent RNA TRIzol Reagent 100 L 15596026 27,600 RNA TRIzol Plus RNA Purification System Invitrogen TRIzol Plus RNA Purification System Purelink RNA Mini Kit No.1 TRIzol 45 RNA TRIzol RNA TRIzol Plus RNA Purification System TRIzol Reagent 100 ml 15596026 PureLink RNA Mini Kit 50 12183018A 200 L 15596018 48,900 4 200 L 15596018SP 178,000 TRIzol LS Reagent 100 L 10296010 27,400 200 L 10296028 48,300 50 12183555 56,400 3. RNA TRIzol Reagent PureLink Kits TRIzol Plus RNA Purification System MagMAX Kits TRIzol+ 60 20 45 45 100 mg 1 10 8 1 ml 200 mg 5 10 6 1 10 8 200 mg 5 10 8 100 mg 5 10 6 10

RNA MagMax RNA RNA Applied Biosystems MagMax RNA MagMAX Total Nucleic Acid Isolation Kit RNA 100 AM1840 115,800 MagMAX-96 Blood RNA Isolation Kit 96 AM1837 73,800 MagMAX-96 Total RNA Isolation Kit 96 AM1830 66,700 MagMAX Viral RNA Isolation Kit 50 AM1939 42,700 MagMAX-96 Viral RNA Isolation Kit 96 AM1836 64,800 RNA Single Place Magnetic Stand 1 AM10026 13,900 Magnetic Stand-96 1 AM10027 67,100 96 well Magnetic-Ring Stand 1 AM10050 139,000 RNA RNAlater Solution Applied Biosystems RNAlater Solution RNA RNAlater Solution RNAlater Solution 100 ml AM7020 18,100 250 ml AM7024 33,800 500 ml AM7021 58,600 50 1.5 ml AM7022 43,400 20 5 ml AM7023 43,400 RNA RNAlater -ICE Applied Biosystems RNAlater -ICE RNA RNAlater -ICE -20 RNA RNAlater -ICE 25 ml AM7030 18,800 RNAqueous Kit Applied Biosystems RNAqueous Kit RNA 20 RNA DNA RNA RNAqueous-4PCR Kit RNAqueous Kit 50 AM1912 40,900 RNAqueous Midi Kit 15 AM1911 29,700 RNAqueous-4PCR Kit 30 AM1914 40,000 RNAqueous-96 Kit 192 AM1920 94,400 RNAqueous-96 Automated Kit 4 96 AM1812 150,600 RNAqueous-Micro Kit 50 AM1931 47,600 DNA TURBO DNA-free Kit Applied Biosystems TURBO DNA-free Kit DNA TURBO DNase TURBO DNase DNase I DNA DNase I 6 RT-PCR false positive DNA TURBO DNA-free Kit 50 AM1907 21,300 RNA THE RNA Storage Solution 10 1 ml AM7000 8,400 50 ml AM7001 8,400 TE Buffer ph7.0 10 1 ml AM9860 8,400 50 ml AM9861 8,400 0.1 mm EDTA 50 ml AM9912 8,400 11

Cells-to-CT Kit RNA Cells-to-CT Kit Applied Biosystems Cells-to-CT Kit RNA PCR RNA Cells-to-CT Kit sirna microrna mirna TaqMan Gene Expression Cells-to-CT Kit TaqMan Fast Cells-to-CT Kit Cells-to-CT 1-Step TaqMan Kit Power SYBR Green Cells-to-CT Kit Cells-to-CT 1-Step Power SYBR Green Kit Fast SYBR Green Cells-to-CT Kit gdna PCR DNase 7 10 100,000 RNA TaqMan PreAmp Cells-to-CT Kit mirna TaqMan MicroRNA Cells-to-CT Kit 細胞溶解 (7 分 ) 逆転写反応リアルタイム解析 2 Step 5. 1 Step RNA 6. Cells-to-CT Kit PCR Cells-to-CT CTs HeLa RNA Cells-to-CT Kit 128 TaqMan Gene Expression Assays Applied Biosystems 7900HT PCR Cells-to-CT Kit Ct RNA Ct Purified RNA C T s 12

TaqMan TaqMan Gene Expression Cells-to-CT Kit Applied Biosystems TaqMan Gene Expression Cells-to-CT Kit RNA DNA cdna PCR TaqMan Gene Expression Master Mix TaqMan Gene Expression Cells-to-CT Kit * 40 4399002 82,400 100 AM1728 162,500 400 AM1729 623,100 TaqMan Cells-to-CT Control Kit/Stop Solution Applied Biosystems TaqMan Cells-to-CT Control Kit TaqMan Gene Expression Cells-to-CT Kit PCR TaqMan Cells-to-CT Control Kit 100 4386995 73,500 Cells-to-CT Stop Solution 1 ml 4402960 35,700 TaqMan Fast Cells-to-CT Kit Applied Biosystems TaqMan Fast Cells-to-CT Kit RNA Fast PCR PCR TaqMan Fast Universal PCR Master Mix TaqMan Fast Cells-to-CT Kit 100 4399003 162,500 * Cells-to-CT 1-Step TaqMan Kit TaqMan 1-Step RT-PCR RT-PCR Applied Biosystems Cellsto-CT 1-Step TaqMan Kit Cells-to-CT Fast 1 RT-PCR Cells-to-CT 1-Step TaqMan Kit 20 A25605 43,200 100 A25603 170,600 400 A25602 654,200 SYBR Green Power SYBR Green / Fast SYBR Green Cells-to-CT Kit Applied Biosystems SYBR Green Cells-to-CT Kits SYBR Green DNA PCR Power SYBR Green Master Mix Fast SYBR Green Master Mix Power SYBR Green Cells-to-CT Kit Power SYBR Green Cells-to-CT Kit 40 4402953 76,900 100 4402954 135,800 400 4402955 517,500 * Fast SYBR Green Cells-to-CT Kit Fast SYBR Green Cells-to-CT Kit 100 4402956 135,800 400 4402957 523,000 * SYBR Green Cells-to-CT Control Kit SYBR Green Cells-to-CT Control Kit 100 4402959 41,500 Cells-to-CT 1-Step Power SYBR Green Kit Applied Biosystems Cells-to-CT 1-Step Power SYBR Green Kit RT-PCR 1 Power SYBR Green 1-Step RT-PCR Cells-to-CT 1-Step 20 A25601 40,300 Power SYBR Green Kit 100 A25600 142,600 400 A25599 543,300 mirna TaqMan MicroRNA Cells-to-CT Kit Applied Biosystems TaqMan MicroRNA Cells-to-CT Kit mirna mirna TaqMan MicroRNA Cells-to-CT Kit 100 4391848 211,400 * TaqMan PreAmp Cells-to-CT Kit Applied Biosystems TaqMan PreAmp Cells-to-CT Kit TaqMan Gene Expression Cells-to-CT Kit TaqMan Gene Expression Master Mix cdna TaqMan PreAmp Master Mix TaqMan PreAmp Cells-to-CT Kit 40 4387299 307,100 * 13

Cell-to-CT Kit Ambion Single Cell-to-CT Kit Invitrogen Ambion Single Cell-to-CT Kit 1 10 RNA 5 Pre-Amplification PCR RNA Ambion Single Cell Lysis Kit RNA Invitrogen Ambion Single Cell Lysis Kit RNA RNA PCR Single Cell-to-CT Kit 28 CT 26 24 22 20 20.4 21.6 1 10µL の Single Cell Solution に 1 10 細胞を加えます室温で 5 分インキュベートします 9. 2 2 1µL の Stop Solution にを加えます室温で 2 分インキュベートします 18 16 14 12 1 Cell Equivalent Single Cells 13.6 100 Cells Ambion Single Cell-to-CT Kit 50 4458237 189,200 400 4458236 1,090,400 Ambion Single Cell Lysis Kit 100 4458235 82,400 * 7. Single Cell-to-CT Kit 100 6.8 CT T DNA Cells-to-CpG Bisulfite Conversion Kit Applied Biosystems Cells-to-CpG Bisulfite Conversion Kit DNA FFPE DNA 8. 10 1. Single Cell Lysis Buffer 25 5 2. PBS / 5 3. PBS RT 95 10 cdna 14 Pre-Amplification B2M GAPDH HPRT1 TGFB1 5 Ct Cells-to-CpG Bisulfite Conversion 50 4445555 36,000 Kit 2 96 4445554 62,000 Cells-to-CpG Bisulfite Conversion 20 4445553 28,500 & Quantitation Control Kit Cells-to-CpG Methylated and Unmethylated gdna Control Kit 50 µl 4445552 42,000 14

1 RT-PCR TaqMan RNA-to-CT 1-Step Kit Applied Biosystems TaqMan RNA-to-CT 1-Step Kit 1 TaqMan 1 1 TaqMan RNA TaqMan Fast Virus 1-Step Master Mix 21 1 AT GC TaqMan Gene Expression Assays 24 TaqMan RNA-to-CT 1-Step Kit, 40 4392653 28,200 1 ml TaqMan RNA-to-CT 1-Step Kit, 200 4392938 85,400 5 ml TaqMan RNA-to-CT 1-Step Kit, 2,000 4392656 773,000 5 ml 10 TaqMan RNA-to-CT 1-Step Kit 1 4393463 TaqMan RNA-to-CT 1-Step Kit 1 4393464 Power SYBR Green RNA-to-CT 1-Step Kit Applied Biosystems Power SYBR Green RNA-to-CT 1-Step Kit PCR 1 SYBR Green Rn 10 1 0.1 0.01 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Cycle Power SYBR Green RNA-to-CT 40 4391178 28,200 1-Step Kit, 1mL Power SYBR Green RNA-to-CT 200 4389986 75,400 1-Step Kit, 5mL Power SYBR Green RNA-to-CT 1 4391003 1-Step Kit Power SYBR Green RNA-to-CT 1-Step Kit 1 4391112 Derivative Reporter (-Rn) 3.5 3.0 2.5 2.0 1.5 1.0 0.5 65.0 70.0 75.0 80.0 85.0 90.0 95.0 Temperature (ºC) 10. Power SYBR Green RNA-to-CT 1-Step Kit GAPDH RNA BOSC23 Applied Biosystems StepOnePlus PCR 3 15

逆転写 PCR SuperScript VILO Invitrogen SuperScript VILO Variable Input Linear Output SuperScript RT PCR mrna cdna RNA SuperScript VILO Master Mix 1 VILO SuperScript VILO PCR cdna PCR 1pg ~ 2.5µg の広範囲で高い直線性逆転写高次構造の RNA テンレー cdna 合成 RNA が高いて逆転写合逆転写性テッでの cpm 30000 25,687 25000 20000 15000 9,310 10000 5000 2,965 0 S 製品 VILO A 製品 11. cdna 32 P-dCTP cdna S A SuperScript VILO VILO A 9 従来高次構造により逆転写が停止 VILO ヘルパータンパク質等により高次構造がほぐれて cdna 合成 13. cdna PCR TaqMan TfR Target SuperScript VILO cdna Synthesis Kit SuperScript VILO cdna Synthesis Kit 50 11754050 37,600 250 11754250 159,400 SuperScript VILO Master Mix 他の製品と比較して最大のダイナミックレンジ SuperScript VILO Master Mix 50 11755050 43,400 250 11755250 182,100 500 11755500 330,100 RNA cdna 合成 RNA cdna 合成 12. RNA cdna VILO RNA cdna 4. High Capacity cdna High Capacity SuperScript VILO SuperScript VILO Reverse Transcriptase Kit RNA-to-cDNA Kit cdna Synthesis Kit Master Mix MultiScribe MultiScribe Superscript III Superscript III 37 C 37 C 42 C 42 C (Total RNA) 20 ng-2 µg 20 ng-2 µg 1 pg-2.5 µg 1 pg-2.5 µg RNase H - - 5 2 2 1 16

High Capacity RNA-to-cDNA Kit Applied Biosystems High Capacity RNA-to-cDNA Kit PCR cdna RNA PCR High Capacity RNA-to-cDNA Kit RNA cdna 2 High Capacity cdna Reverse Transcription Kit High Capacity cdna Reverse 200 4368814 33,300 Transcription Kit 1,000 4368813 140,600 High Capacity cdna Reverse 200 4374966 41,700 Transcription Kit, with RNase Inhibitor 1,000 4374967 188,800 High Capacity cdna Reverse 1 4375575 Transcription Kit RNase Inhibitor, 20 units/µl 100 N8080119 14,400 *20 µl 30 60 2µg RNA 2 RT-PCR High Capacity RNA-to-cDNA Kit 50 4387406 22,300 High Capacity RNA-to-cDNA Kit 1 4387951 High Capacity RNA-to-cDNA Kit 1 4392339 *20 µl TaqMan Reverse Transcriation Reagents TaqMan Reverse 200 N8080234 64,600 Transcription Reagents 10-Pack, TaqMan Reverse Transcription Reagents 2,000 4304134 561,900 / / MultiScribe Reverse Transcriptase 5,000 units 4311235 13,700 RNase Inhibitor, 20 units/µl 2,000 units N8080119 14,400 Random Hexamers, 5 nmoles 0.1 ml N8080127 12,500 Oligo d(t) 16 Primers, 5 nmoles 0.1 ml N8080128 12,500 GeneAmp dntp Blend, 10 mm 1 ml N8080260 11,200 GeneAmp dntp Blend, 12.5 mm 100 N8080270 17,100 with dutp AmpErase Uracil N-glycosylase 100 units N8080096 27,900 UNG 20 % Glycerol Solution, 100 ml 402929 13,500 Molecular Biology Grade TaqMan Control Total RNA Human 100 µl 4307281 15,800 50 ng/µl TaqMan Control Genomic DNA Human 10 ng/µl, 2 100 µl 4312660 13,500 17

TaqMan Fast Fast 10 PCR 40 45 Fast Fast 40 Applied Biosystems TaqMan Fast Advanced Master Mix 55 40 Fast Fast PCR Fast Fast PCR ΔRn 1 0.1 0.01 12 14 16 18 20 22 24 26 28 30 Cycle Number TaqMan Fast Advanced Master Mix 40 TaqMan Fast Universal PCR Master Mix Applied Biosystems TaqMan Fast Universal PCR Master Mix TaqMan Fast PCR Fast PCR Fast PCR Fast AmpliTaq Fast DNA Polymerase 72 PCR TaqMan Assays TaqMan Array Fast Fast Fast TaqMan Fast Universal PCR 250 4352042 36,000 Master Mix 2X, No AmpErase UNG 2-Pack, TaqMan Fast Universal PCR 500 4366072 69,700 Master Mix 2X, No AmpErase UNG 5-Pack, TaqMan Fast Universal PCR 1,250 4366073 173,100 Master Mix 2X, No AmpErase UNG 10-Pack, TaqMan Fast Universal PCR 2,500 4364103 330,400 Master Mix 2X, No AmpErase UNG TaqMan Fast Universal PCR 5,000 4367846 595,500 Master Mix 50 ml TaqMan Fast Universal PCR 1 4351891 Master Mix *20 µl TaqMan Fast Advanced Master Mix, 100 4444556 15,800 1 ml TaqMan Fast Advanced Master Mix, 500 4444557 70,900 5 ml TaqMan Fast Advanced Master Mix, 1,000 4444963 137,100 2 5 ml TaqMan Fast Advanced Master Mix, 2,500 4444964 331,500 5 5 ml TaqMan Fast Advanced Master Mix, 5,000 4444965 640,400 10 5 ml TaqMan Fast Advanced Master Mix, 5,000 4444558 627,000 50 ml *20 µl 18

TaqMan 1 TaqMan Universal Master Mix II Applied Biosystems TaqMan Universal Master Mix II microrna mirna SNP CNV Applied Biosystems TaqMan Universal Master Mix II 1 RNase P TaqMan Universal Master Mix II PCR PCR 24 SNP mirna TaqMan Assays TaqMan Assays PCR UNG 2 14. PCR TaqMan Universal Master Mix II, with UNG TaqMan Universal Master Mix II, 40 4440042 15,800 with UNG 1 ml TaqMan Universal Master Mix II, 200 4440038 70,900 with UNG 5 ml TaqMan Universal Master Mix II, 400 4440044 137,100 with UNG 2 5 ml TaqMan Universal Master Mix II, 1,000 4440045 331,500 with UNG 5 5 ml TaqMan Universal Master Mix II, 2,000 4440046 640,400 with UNG 10 5 ml TaqMan Universal Master Mix II, with UNG 50 ml 2,000 4440039 627,000 TaqMan Universal Master Mix II, no UNG TaqMan Universal Master Mix II, 40 4440043 15,800 no UNG 1 ml TaqMan Universal Master Mix II, 200 4440040 65,200 no UNG 5 ml TaqMan Universal Master Mix II, 400 4440047 125,900 no UNG 2 5 ml TaqMan Universal Master Mix II, 1,000 4440048 303,400 no UNG 5 5 ml TaqMan Universal Master Mix II, 2,000 4440049 573,000 no UNG 10 5 ml TaqMan Universal Master Mix II, no UNG 50 ml 2,000 4440041 561,800 *50 µl PCR 24 PCR TaqMan Universal Master Mix II PCR PCR 30 24 15. 24 19

TaqMan TaqMan Gene Expression Master Mix Applied Biosystems TaqMan Gene Expression Master Mix PCR GC AT PCR TaqMan Universal PCR Master Mix 72 TaqMan Gene Expression 40 4370048 15,800 Master Mix, 1 ml TaqMan Gene Expression 200 4369016 70,900 Master Mix, 5 ml TaqMan Gene Expression 400 4369514 137,100 Master Mix, 2 5 ml TaqMan Gene Expression 1,000 4369510 331,500 Master Mix, 5 5 ml TaqMan Gene Expression 2,000 4369542 640,400 Master Mix, 10 5 ml TaqMan Gene Expression 2,000 4370074 627,000 Master Mix, 50 ml TaqMan Gene Expression 1 4371135 Master Mix TaqMan Gene Expression 1 4371134 Master Mix *50 µl RNA PCR Water Nuclease free Water Nuclease-free Water 500 ml AM9930 14,000 1,000 ml AM9932 23,300 10 50 ml AM9937 17,600 100 ml AM9938 6,000 5 100 ml AM9939 20,900 TaqMan Universal PCR Master Mix Applied Biosystems TaqMan Universal PCR Master Mix TaqMan 5' PCR Applied Biosystems AmpliTaq Gold DNA Polymerase TaqMan Universal PCR Master Mix 200 4304437 76,400 2-Pack, 400 4364338 140,500 TaqMan Universal PCR Master Mix 4304437 2 5-Pack, 1,000 4364340 342,800 TaqMan Universal PCR Master Mix 4304437 5 10-Pack, 2,000 4305719 674,100 TaqMan Universal PCR Master Mix 4304437 10 TaqMan Universal PCR Master Mix, 10-Pack 5 ml 10 2,000 4318157 674,100 TaqMan Universal PCR Master Mix, 50 2,000 4326708 662,900 ml TaqMan Universal PCR Master Mix 1 4304449 *50 µl TaqMan Universal PCR Master Mix, No AmpErase UNG Applied Biosystems TaqMan Universal PCR Master Mix, No AmpErase UNG AmpErase UNG TaqMan 5' PCR AmpliTaq Gold DNA Polymerase TaqMan Universal PCR Master Mix, 200 4324018 70,900 No AmpErase UNG TaqMan Universal PCR Master Mix, 400 4364341 130,400 No AmpErase UNG, 2-Pack TaqMan Universal PCR Master Mix, 1,000 4364343 312,400 No AmpErase UNG, 5-Pack TaqMan Universal PCR Master Mix, 2,000 4324020 606,700 No AmpErase UNG, 10-Pack TaqMan Universal PCR Master Mix, 2,000 4326614 595,500 No AmpErase UNG, 50 ml TaqMan Universal PCR Master Mix 1 4304449 *50 µl 20

PCR TaqMan Fast Virus 1-Step Master Mix PCR RNA DNA 1 4 RNA / DNA PCR Fast RT-PCR PCR TaqMan PreAmp Master Mix Applied Biosystems TaqMan PreAmp Master Mix cdna PrePCR PrePCR TaqMan Gene Expression Assays 100 1ng cdna 200 PCR TaqMan PreAmp Master Mix 40 4391128 139,400 TaqMan PreAmp Master Mix Kit 1 4384267 177,600 PreAmp Master Mix (4391128) TaqMan Gene Expression Master Mix, 5 ml (4369016) PCR Applied Biosystems TaqMan Fast Virus 1-Step Master Mix TaqMan Reagents Starter Kit TaqMan Reagents Starter Kit 1 Kit 4352405 39,400 TaqMan Universal Master Mix, No UNG 1.25 ml TaqMan Gene Expression Assay, Eukaryotic 18S rrna, 20X 0.3 ml Human Raji cdna, 25 ng/µl 0.05 ml Fast PCR TaqMan Fast Virus 1-Step Master Mix PCR 4 Fast 一般的な 2 ステップ反応 TaqMan EZ RT-PCR Core Reagents TaqMan RNA-to-C T 1-Step Kit TaqMan Fast Virus 1-Step Master Mix 0 20 40 60 80 100 120 140 リアルタイム PCR の所要時間 ( 分 ) 16. 4 Fast TaqMan Fast Virus 1-Step Master Mix 1 1 TaqMan Fast Virus 1-Step Master Mix TaqMan Fast Virus 1-Step Master Mix, 1 ml TaqMan Fast Virus 1-Step Master Mix, 5 1 ml TaqMan Fast Virus 1-Step Master Mix, 10 ml *20 µl 200 4444432 55,800 1,000 4444434 170,800 2,000 4444436 331,500 21

TaqMan TaqMan Multiplex Master Mix Applied Biosystems TaqMan Multiplex Master Mix Fast PCR 4 TaqMan Multiplex Master Mix AmpliTaq Fast DNA Polymerase Uracil-N Glycosylase UNG dutp dntp MUSTANG PURPLE 2X 4 SNP Fast PCR Standard PCR Fast PCR UNG UDG TaqMan Multiplex Master Mix 1 ml 4461881 22,100 5 ml 4461882 92,100 2 x 5 ml 4461884 171,400 5 x 5 ml 4484262 397,800 10 x 5 ml 4484263 704,500 50 ml 4486295 689,700 TaqMan GAPDH Assay, 250 reactions 4485712 45,000 JUN dye/qsy probe TaqMan GAPDH Assay, 250 reactions 4485713 45,000 JUN dye/qsy probe, primer-limited TaqMan RNase P Assay, 250 reactions 4485714 45,000 ABY dye/qsy probe TaqMan RNase P Assay, ABY dye/qsy probe, primer-limited 250 reactions 4485715 45,000 TaqMan QSY TaqMan QSY 6,000 pmol 4482777 58,500 20,000 pmol 4482778 110,200 50,000 pmol 4482779 153,300 QSY www.lifetechnologies.com/taqman-ord A Rn B Rn C Rn D Rn 6 5 4 3 2 1 3.5 3.0 2.5 2.0 1.5 1.0 0.5 3.0 2.5 2.0 1.5 1.0 0.5 3.0 2.5 2.0 1.5 1.0 0.5 Amplification plot 8 2 10 20 30 4 12 22 32 6 14 24 34 16 26 36 18 28 38 40 Cycle Amplification plot 8 2 10 20 30 4 12 22 32 6 14 24 34 16 26 36 18 28 38 40 Cycle Amplification plot 8 2 10 20 30 4 12 22 32 6 14 24 34 16 26 36 18 28 38 40 Cycle Amplification plot 8 2 10 20 30 4 12 22 32 6 14 24 34 16 26 36 18 28 38 40 Cycle 17. A EGFR FAM B BRCA1 VIC C ESR1 JUN D RNaseP ABY QuantStudio 7 PCR TaqMan Multiplex Master Mix cdna 10 µl 20,000 pg 2 pg 4 4-plex Ct 4-plex PCR EGFR 96.09% 4-plex 96.39% BRCA1 93.56% 4-plex 94.93% ESR1 97.13% 4-plex 95.81% RNaseP 96.91% 4-plex 98.1% TaqMan QSY Applied Biosystems TaqMan QSY 4 3 MGB QSY 22

SYBR Green Power SYBR Green PCR Master Mix Applied Biosystems Power SYBR Green PCR Master Mix 2 DNA cdna RNA PCR SYBR Green PCR 2 Power SYBR Green PCR Master Mix, 1 ml Power SYBR Green PCR Master Mix, 5 ml 2-Pack, Power SYBR Green PCR Master Mix, 2 5 ml 5-Pack, Power SYBR Green PCR Master Mix, 5 5 ml 10-Pack, Power SYBR Green PCR Master Mix, 10 5 ml Power SYBR Green PCR Master Mix, 50 ml Power SYBR Green PCR Master Mix *50 µl 40 4368577 9,500 200 4367659 36,800 400 4368706 71,400 1,000 4368702 173,300 2,000 4368708 336,000 2,000 4367660 315,000 1 4367218 Fast SYBR Green Master Mix Applied Biosystems Fast SYBR Green Master Mix Fast PCR Fast PCR PCR Fast SYBR Green Master Mix, 1 ml 100 4385610 9,500 Fast SYBR Green Master Mix, 5 ml 500 4385612 36,800 2-Pack, Fast SYBR Green Master Mix, 2 5 ml 5-Pack, Fast SYBR Green Master Mix, 5 5 ml 10-Pack, Fast SYBR Green Master Mix, 10 5 ml 1,000 4385616 71,400 2,500 4385617 173,300 5,000 4385618 336,000 Fast SYBR Green Master Mix, 50 ml 5,000 4385614 315,000 Fast SYBR Green Master Mix Fast SYBR Green Master Mix *20 µl 1 4385371 1 4385372 Delta Rn vs Cycle 1.0e+002 Delta Rn 1.0e+001 1.0e+000 1.0e-001 1.0e-002 50 ng 5 ng 500 pg 50 pg 5 pg 5 10 15 20 25 30 35 40 Cycle Number 19. Fast SYBR Master Mix SYBR Green PCR Master Mix Applied Biosystems SYBR Green PCR Master Mix SYBR Green PCR 2 CT Standard Curve 34 32 5 pg Slope = -3.28 30 50 pg 28 R 2 = 0.997 26 500 pg 24 22 5 ng 20 50 ng 18 16 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 Log Concentration SYBR Green PCR Master Mix, 1 ml 40 4344463 10,500 SYBR Green PCR Master Mix, 5 ml 200 4309155 51,500 SYBR Green PCR Master Mix, 50 ml 2,000 4334973 446,300 10-Pack, SYBR Green PCR Master Mix, 10 5 ml SYBR Green PCR Master Mix *50 µl 2,000 4312704 462,000 1 4310251 18. Human gdna -actin 23

TaqMan TaqMan Gene Expression Assays 5. TaqMan Gene Expression Assays 2011 10 Applied Biosystems TaqMan Gene Expression Assays PCR & 130 Exon RNAi FAM TaqMan Gene Expression Assays, 250 4331182 33,500 Inventoried TaqMan Gene Expression Assays, 360 4351372 50,700 Small, Made-to-Order TaqMan Gene Expression Assays, 750 4351370 84,000 Medium, Made-to-Order TaqMan Gene Expression Assays, 2,900 4351368 166,900 Large, Made-to-Order VIC TaqMan Gene Expression Assays, 360 4448489 50,700 Small_VIC, Made-to-Order TaqMan Gene Expression Assays, 750 4448490 84,000 Medium_VIC, Made-to-Order TaqMan Gene Expression Assays, Large_VIC, Made-to-Order 2,900 4448491 166,900 Primer Limited TaqMan Assays VIC TaqMan Gene Expression Assays, 360 4448484 50,700 Small_VIC, PL Made-to-Order TaqMan Gene Expression Assays, 750 4448485 84,000 Medium_VIC, PL Made-to-Order TaqMan Gene Expression Assays, Large_VIC, PL Made-to-Order 2,900 4448486 166,900 *20 µl Inventoried Made-to-Order 60 www.lifetechnologies.com/taqmanjp Species Number of Assays H. sapiens (Human) 212,207 M. musculus (Mouse) 185,566 R. norvegicus (Rat) 151,955 O. sativa (Rice) 99,822 A. thaliana (Arabidopsis) 97,879 C. elegans (Nematode) 92,687 B. taurus (Cow) 67,104 M. mulatta (Rhesus Monkey) 66,480 D. rerio (Zebrafish) 63,712 X. tropicalis (Frog) 56,764 C. familiaris (Dog) 55,202 G. gallus (Chicken) 48,432 Species Number of Assays D. melanogaster (Fruit Fly) 41,607 Z. mays (Sweet Corn) 38,446 S. scrofa (Pig) 9,868 S. pombe (Fission Yeast) 6,538 O. cuniculus (Rabbit) 5,924 S. cerevisiae (Baker's Yeast) 5,525 G. max (Soybean) 3,456 E. caballus (Horse) 2,970 C. porcellus (Guinea Pig) 2,037 V. vinifera (Grape) 965 T. aestivum (Wheat) 760 Total Number of Assays 1,315,906 Custom TaqMan Gene Expression Assays Applied Biosystems Custom TaqMan Gene Expression Assays TaqMan Gene Expression Assays TaqMan Gene Expression Assays TaqMan Custom TaqMan Gene 50 µl 140 4331348 50,700 Expression Assays, S-scale 20 µl 360 Custom TaqMan Gene Expression Assays, M-scale Custom TaqMan Gene Expression Assays, L-scale 50 µl 300 20 µl 750 50 µl 1,160 20 µl 2,900 4332078 102,100 4332079 181,600 Custom Plus TaqMan RNA Assays Applied Biosystems Custom Plus TaqMan RNA Assays TaqMan Gene Experssion Assays Custom Plus TaqMan RNA Assays Custom Plus TaqMan RNA Assay, 360 4441114 50,700 Small 20 Custom Plus TaqMan RNA Assay, Medium 20 750 4441117 102,100 Custom Plus TaqMan RNA Assay, Large 60 2,900 4441118 181,600 24

TaqMan 96 TaqMan Array Plate 96 - Fast Applied Biosystems TaqMan Array Plate TaqMan Gene Expression Assays 24 96 5 Fast 10 TaqMan Gene Expression Assays 6 96 PCR TaqMan Standard : 7900HT / ViiA 7 / 7500 / 7300 / QuantStudio 96 Fast : 7900HT / ViiA 7 /7500Fast / StepOnePlus /Quant- Studio Fast96 60 TaqMan Array Plate www.lifetechnologies.com/taqmanjp ** * TaqMan Array Plates, Format 8 (Standard) TaqMan Array Plates, Format 8 (Fast) TaqMan Array Plates, Format 16 (Standard) TaqMan Array Plates, Format 16 (Fast) TaqMan Array Plates, Format 16 + Endogenous Controls (Standard) TaqMan Array Plates, Format 16 + Endogenous Controls (Fast) TaqMan Array Plates, Format 32 (Standard) TaqMan Array Plates, Format 32 (Fast) TaqMan Array Plates, Format 32 + Endogenous Controls (Standard) TaqMan Array Plates, Format 32 + Endogenous Controls (Fast) TaqMan Array Plates, Format 48 (Standard) TaqMan Array Plates, Format 48 (Fast) TaqMan Array Plates, Format 48 + Endogenous Controls (Standard) TaqMan Array Plates, Format 48 + Endogenous Controls (Fast) TaqMan Array Plates, Format 96 (Standard) TaqMan Array Plates, Format 96 (Fast) TaqMan Array Plates, Format 96 + Endogenous Controls (Standard) TaqMan Array Plates, Format 96 + Endogenous Controls (Fast) 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 4413266 34,100 29,600 25,000 4413263 34,100 29,600 25,000 4413264 38,700 34,100 29,600 4413261 38,700 34,100 29,600 4413265 38,700 34,100 29,600 4413262 38,700 34,100 29,600 4391528 51,200 44,300 38,700 4413259 51,200 44,300 38,700 4391529 51,200 44,300 38,700 4413260 51,200 44,300 38,700 4391526 56,800 51,200 44,300 4413257 56,800 51,200 44,300 4391527 56,800 51,200 44,300 4413258 56,800 51,200 44,300 4391524 67,000 56,800 51,200 4413255 67,000 56,800 51,200 4391525 67,000 56,800 51,200 4413256 67,000 56,800 51,200 * 1 6 6 12 18 24 ** Candidate Endogenous Control GAPDH HPRT1 GUSB 3 25

TaqMan 96 TaqMan Array Gene Signature 96-Well Plate Applied Biosystems TaqMan Array Gene Signature 96-Well Plate TaqMan Gene Expression Assays 96 1 96 Standard Fast Human Alzheimer's Disease 4414070 4418715 Human Angiogenesis 4414071 4418716 Human Apoptosis 4414072 4418717 Human Immune Response 4414073 4418718 Human Inflammation 4414074 4418719 Human Phosphodiesterase 4414075 4418720 Human Protein Kinase Pathways 4414076 4418721 Human Stem Cell Pluripotency 4414077 4418722 Mouse Alzheimer's Disease 4414078 4418723 Mouse Immune Response 4414079 4418724 Mouse Stem Cell Pluripotency 4414080 4418725 Rat Inflammation 4414081 4418726 Rat Phosphodiesterase 4414082 4418727 Human Androgens 4414083 4418728 Human Chemokines 4414084 4418729 Human CYP450 & other Oxygenases 4414085 4418730 Human Diabetes 4414086 4418731 Human Estrogens 4414087 4418732 Human Heat Shock Proteins 4414088 4418733 Human Hedgehog Pathway 4414089 4418734 Human Hypoxia 4414090 4418735 Human LDL Pathways 4414091 4418736 Human Ligand Gated Ion Channels 4414092 4418737 Human MAPK Pathways 4414093 4418738 Human Neurotransmitters 4414094 4418739 Human NFkB Pathway 4414095 4418740 Human Osteogenesis 4414096 4418741 Human TGF Pathway 4414097 4418742 Human Tumor Metastasis 4414098 4418743 Human Voltage-Gated Ion Channels 4414099 4418744 Human WNT Pathway 4414100 4418745 Human 14-3-3 Induced Intracellular Signaling 4414101 4418746 Human Activation of camp-dependent PKA 4414102 4418747 Human HOX Genes 4414103 4418748 Human Antigen Processing and Presentation 4414104 4418749 by MHCS Human Apoptosis via Death Receptors 4414105 4418750 Human Apoptotic Pathways Triggered By HIV1 4414106 4418751 Mouse ABC Transporters 4414107 4418752 Human Nuclear Receptors 4414108 4418753 Human Cell Surface Markers 4414109 4418754 Human BMP Pathway 4414110 4418755 1 38,700 Standard Fast Human BRCA1 Pathway 4414111 4418756 Human Breast Cancer Regulations by Stathmin1 4414112 4418757 Human c-myc and Apoptosis 4414113 4418758 Human camp Pathway 4414114 4418759 Human Cardiomyocyte Differentiation 4414115 4418760 by BMP Receptors Human Cell Cycle Control of Chromosomal 4414116 4418761 Replication Human Cellular Apoptosis Pathway 4414117 4418762 Human Drug Transporters 4414118 4418763 Human Antioxidant Mechanisms 4414119 4418764 Human Complement Pathway 4414120 4418765 Human Colorectal Cancer Metastasis 4414121 4418766 Human CREB Pathway 4414122 4418767 Human Cyclins & Cell Cycle Regulation 4414123 4418768 Human Cytokine Network 4414124 4418769 Human Developmental Phases of Colorectal 4414125 4418770 Cancer Human DNA Damage Induced 14-3-3 Sigma 4414126 4418771 Signaling Human DNA Methylation & Transcriptional 4414127 4418772 Repression Human DNA Repair Mechanisms 4414128 4418773 Human EGF Pathway 4414129 4418774 Human Signal Transduction Pathways 4414130 4418775 Human enos Signaling 4414131 4418776 Human Erythropoietin Pathway 4414132 4418777 Human Extracellular Matrix & Adhesion Molecules 4414133 4418778 Human Factors for Cardiogenesis 4414134 4418779 Human FAS Signaling 4414135 4418780 Human FGF Pathway 4414136 4418781 Human GPCR Pathway 4414137 4418782 Human Guidance & Growth Cone Motility 4414138 4418783 Human Transcription Factors (Non-Hox) 4414139 4418784 Human Huntington Disease Pathway 4414140 4418785 Human IGF1R Signaling 4414141 4418786 Human IL-1 Pathway 4414142 4418787 Human IL-10 Pathway 4414143 4418788 Human IL-2 Active 8 & Quiescent T-Cell 4414144 4418789 Human IL-2 Pathway 4414145 4418790 Human AHR Pathway 4414146 4418791 Human IL-3 Pathway 4414147 4418792 Human IL-4 Pathway 4414148 4418793 26

Standard Fast Human IL-6 Pathway 4414149 4418794 Human IL-9 Pathway 4414150 4418795 Human ILK Signaling 4414151 4418796 Human inos Signaling 4414152 4418797 Human Insulin Receptor Pathway 4414153 4418798 Human Interferon Pathway 4414154 4418799 Human Intracellular Calcium Signaling 4414155 4418800 Human JAK-STAT Pathway 4414156 4418801 Human Growth Factors 4414157 4418802 Human Hematopoiesis 4414158 4418803 Human Melatonin Signaling 4414159 4418804 Human Prion Pathway 4414160 4418805 Human Molecular Mechanisms of Cancer 4414161 4418806 Human NGF Pathway 4414162 4418807 Human nnos Signaling at Neuronal Synapses 4414163 4418808 Human nnos Signaling in Skeletal Muscle 4414164 4418809 Human Notch Signaling 4414165 4418810 Human ABC Transporters 4414166 4418811 Human P53 Mediated Apoptosis 4414167 4418812 Human P53 Signaling 4414168 4418813 Human CMV & MAPK Pathways 4414169 4418814 Human Patho Rheumatoid Arthritis 4414170 4418815 Human PDGF Pathway 4414171 4418816 Human PI3K Signaling 4414172 4418817 Human PI3K Signaling in B-Lymphocyte 4414173 4418818 Human PKA Signaling 4414174 4418819 Human Cholera Infection 4414175 4418820 Human RAS Pathway 4414176 4418821 Human Pancreatic Adenocarcinoma 4414177 4418822 Human Phagocytosis of Microbes 4414178 4418823 Human RHO Family GTPases 4414179 4418824 Human RHOA Pathway 4414180 4418825 Human Pathogenesis of ALS 4414181 4418826 Human Signaling in Gap Junctions 4414182 4418827 Human NFAT & Cardiac Hypertrophy 4414183 4418828 Human SMAD Signaling Network 4414184 4418829 Human Sperm Motility 4414185 4418830 Human T-Cell Receptor and CD3 Complex 4414186 4418831 Human Telomere Extension by Telomerase 4414187 4418832 Human Thrombin Signaling 4414188 4418833 Human TNF Superfamily Pathway 4414189 4418834 Human TNFR1 Pathway 4414190 4418835 1 38,700 Standard Fast Human TNFR2 Pathway 4414191 4418836 Human Toll Comparative Pathway 4414192 4418837 Human Toll-Like Receptors Pathway 4414193 4418838 Human Transcription of mrna 4414194 4418839 Human Transcription of rrna 4414195 4418840 Human Transcription of trna 4414196 4418841 Human Transcriptional Regulatory Network 4414197 4418842 in Embryonic Stem Cell Human Ubiquitin-Proteasome Dependent 4414198 4418843 Proteolysis Human VEGF Pathway 4414199 4418844 Human Maturity-Onset Diabetes of Young MODY 4414200 4418845 Mouse Lipid Regulated Genes 4415461 4418846 Rat Endogenous Control 4426698 4426697 Mouse Endogenous Control 4426701 4426699 Human Endogenous Control 4396840 4426700 27

TaqMan 384 TaqMan Gene Signature Arrays Applied Biosystems TaqMan Gene Signature Arrays 384 14 TaqMan 384 384 5 Human Human Immune Array 4 4370573 227,000 Human Endogenous Control Array 2 4367563 102,100 Human Protein Kinase Array 4 4367784 227,000 Human GPCR Array 4 4367785 249,700 Human ABC Transporter Array 4 4378700 227,000 Human Apoptosis Array 4 4378701 227,000 Human Phosphodiesterase Array 4 4378705 227,000 Human Inflammation Array 4 4378707 227,000 Human Angiogenesis Array 4 4378710 227,000 Human Alzheimer's Array 4 4378713 227,000 Human Nuclear Receptor Array 4 4379961 227,000 Human Stem Cell Pluripotency Array 4 4385344 227,000 TaqMan Array Human MicroRNA A Cards v2.0 4 4398965 227,000 TaqMan Arrays Human MicroRNA B Cards v3.0 4 4444910 227,000 TaqMan Arrays Human MicroRNA A+B Cards v3.0 8 4444913 292,800 www.lifetechnologies.com Mouse Mouse Immune Array 4 4367786 227,000 Mouse Endogenus Control Array 2 4378702 102,100 Mouse GPCR Array 4 4378703 249,700 Mouse Alzheimer's Array 4 4378714 227,000 Mouse Stem Cell Pluripotency Array 4 4385363 227,000 TaqMan Array Rodent MicroRNA A Cards v2.0 4 4398967 227,000 Rat Rat Endogenous Control Array 2 4378704 102,100 Rat Phosphodiesterase Array 4 4378706 227,000 Rat Inflammation Array 4 4378708 227,000 Rat GPCR Array 4 4378709 249,700 TaqMan Array Rodent MicroRNA A Cards v2.0 4 4398967 227,000 TaqMan Custom Arrays Micro Fluidic Card Applied Biosystems TaqMan Custom Arrays 7900HT Fast ViiA 7 QuantStudio PCR 384 1 12 384 1 8 TaqMan Custom Arrays 7900HT ViiA 7 QuantStudio TaqMan Array Micro Fluidic Card 2 µl 384 TaqMan Gene Signature Arrays 10 19 20 49 50 99 100 TaqMan Array, Format 12 4342247 77,200 63,600 51,200 45,400 TaqMan Array, Format 16 4346798 79,400 65,900 52,300 46,600 TaqMan Array, Format 24 4342249 79,400 65,900 52,300 46,600 TaqMan Array, Format 32 4346799 84,000 70,300 54,500 48,800 TaqMan Array, Format 48 4342253 84,000 70,300 54,500 48,800 TaqMan Array, Format 64 4346800 86,300 72,600 56,800 49,900 TaqMan Array, Format 96a 4342259 88,500 73,900 63,600 52,300 TaqMan Array, Format 96b 4342261 88,500 73,900 63,600 52,300 TaqMan Array, Format 192 4346802 110,200 81,700 70,300 56,800 TaqMan Array, Format 384 4342265 140,900 95,300 74,900 61,300 * TaqMan Gene Expression Assays, Inventoried 24 TaqMan Gene Expression Assays, Made-to-Order 24 60 28

TaqMan TaqMan Array Endogenous Control Plate Applied Biosystems TaqMan Array Endogenous Control Plate 32 32 3 TaqMan TaqMan Array Human Endogenous 1 4396840 38,700 Controls, 96-well Plate TaqMan Array Human Endogenous 1 4426700 38,700 Controls, Fast 96-Well Plate TaqMan Array Mouse Endogenous 1 4426701 38,700 Controls, 96-well Plate TaqMan Array Mouse Endogenous 1 4426699 38,700 Controls, Fast 96-Well Plate TaqMan Array Rat Endogenous 1 4426698 38,700 Controls, 96-well Plate TaqMan Array Rat Endogenous Controls, Fast 96-Well Plate 1 4426697 38,700 6. TaqMan Array Human Endogenous Control Plate Assay ID Gene Symbol TaqMan Human Endogenous Control Array Applied Biosystems TaqMan Human Endogenous Control Array TaqMan Gene Signature Arrays 28 16 Gene Expression Assays TaqMan Human Endogenous Control Array 7900HT ViiA 7 QuantStudio TaqMan Array TaqMan Human Endogenous 2 4367563 102,100 Control Array 29

TaqMan TaqMan Gene Expression Assays Endogenous Control Applied Biosystems TaqMan Gene Expression Assays 24 60 TaqMan Endogenous Controls Applied Biosystems TaqMan Endogenous Controls VIC FAM TaqMan Gene Expression 50 µl 100 4331182 33,500 Assays, Inventoried 20 µl 250 TaqMan Endogenous Control 1,000 ** 111,300 VIC/MGB * 50 µl ** Human Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC/MGB FAM/MGB 18S X03205.1 Eukaryotic 18S rrna Hs99999901_s1 4319413E 4352930E ACTB NM_001101.2 actin, beta Hs99999903_m1 4326315E 4352935E GAPDH NM_002046.3 glyceraldehyde-3-phosphate dehydrogenase Hs99999905_m1 4326317E 4352934E RPLP0 NM_001002.3 ribosomal protein, large, P0 Hs99999902_m1 4326314E PPIA NM_021130.3 peptidylprolyl isomerase A (cyclophilin A) Hs99999904_m1 4326316E PGK1 NM_000291.2 phosphoglycerate kinase 1 Hs99999906_m1 4326318E B2M NM_004048.2 beta-2-microglobulin Hs99999907_m1 4326319E GUSB NM_000181.1 glucuronidase, beta Hs99999908_m1 4326320E HPRT1 NM_000194.1 hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome) Hs99999909_m1 4326321E TBP M55654.1 TATA box binding protein Hs99999910_m1 4326322E TFRC NM_003234.1 transferrin receptor (p90, CD71) Hs99999911_m1 4326323E IPO8 NM_006390.2 importin 8 Hs00183533_m1 YWHAZ NM_003406.2 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Hs00237047_m1 UBC NM_021009.4 ubiquitin C Hs00824723_m1 POLR2A NM_000937.3 polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Hs00172187_m1 HMBS NM_000190.3 hydroxymethylbilane synthase Hs00609297_m1 * VIC TaqMan Fast Universal Master Mix / 60 62 30

Mouse Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC/MGB FAM/MGB 18S X03205.1 Eukaryotic 18S rrna Hs99999901_s1 4319413E 4352930E ACTB NM_007393.1 actin, beta, cytoplasmic Mm00607939_s1 4352341E 4352933E Gapdh NM_008084.2 glyceraldehyde-3-phosphate dehydrogenase Mm99999915_g1 4352339E 4352932E Rplp2 (AK012402.1) ribosomal protein, large P2 Mm00782638_s1 Ppia NM_008907.1 peptidylprolyl isomerase A Mm02342430_g1 Pgk1 NM_008828.2 phosphoglycerate kinase 1 Mm00435617_m1 B2m NM_009735.3 beta-2 microglobulin Mm00437762_m1 Gusb NM_010368.1 glucuronidase, beta Mm00446953_m1 Hprt1 NM_013556.2 hypoxanthine guanine phosphoribosyl transferase 1 Mm00446968_m1 Tbp NM_013684.2 TATA box binding protein Mm00446973_m1 Tfrc NM_011638.3 transferrin receptor Mm00441941_m1 Ipo8 NM_001081113.1 importin 8 Mm01255158_m1 Ywhaz NM_011740.2 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Mm01158417_g1 Ubc ubiquitin C Mm01201237_m1 Polr2a NM_009089.2 polymerase (RNA) II (DNA directed) polypeptide A Mm00839493_m1 Hmbs NM_013551.2 hydroxymethylbilane synthase Mm00660262_g1 Rat Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC/MGB FAM/MGB 18S X03205.1 Eukaryotic 18S rrna Hs99999901_s1 4319413E 4352930E Actb NM_031144.2 actin, beta Rn00667869_m1 4352340E 4352931E Gapdh NM_017008.3 glyceraldehyde-3-phosphate dehydrogenase Rn99999916_s1 4352338E Rplp2 NM_001030021.1 ribosomal protein, large P2 Rn01479927_g1 Ppia NM_017101.1 peptidylprolyl isomerase A Rn00690933_m1 Pgk1 NM_053291.3 phosphoglycerate kinase 1 Rn00821429_g1 B2m NM_012512.1 beta-2 microglobulin Rn00560865_m1 Gusb NM_017015.2 glucuronidase, beta Rn00566655_m1 Hprt NM_012583.2 hypoxanthine guanine phosphoribosyl transferase Rn01527840_m1 Tbp NM_001004198.1 TATA box binding protein Rn01455648_m1 Tfrc M58040.1 transferrin receptor Rn01474695_m1 Hmbs NM_013168.2 hydroxymethylbilane synthase Rn00565886_m1 Ywhaz NM_013011.3 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Rn00755072_m1 Ubc NM_017314.1 ubiquitin C Rn01789812_g1 Ppib NM_022536.1 peptidylprolyl isomerase B Rn00574762_m1 Arbp NM_022402.1 acidic ribosomal phosphoprotein P0 Rn00821065_g1 * VIC TaqMan Fast Universal Master Mix / 60 62 31

RNAi RNAi RNA RNAi RNA 2 RNA dsrna dsrna mrna RNAi RNAi RNAi RNA dsrna RNAi sirna Dicer dsrna 21 23 sirna small interfering RNA sirna RISC RNA-induced silencing complexes sirna RISC mrna RISC mrna 30 dsrna sirna Dicer sirna shrna short hairpin RNA DNA RNAi 1. sirna RNAi 2 sirna sirna sirna 33 35 2. sirna sirna sirna sirna sirna sirna 36 3. sirna mrna sirna PCR TaqMan mrna TaqMan 18 21 TaqMan 24 31 20. RNAi 32

sirna Silencer Select sirna Invitrogen Silencer Select sirna LNA sirna sirna 1/10 1/100 5nM LNA 21. Silencer Select sirna sirna 10 sirna 0.03 30nM sirna HeLa sirna triplicate 48 mrna sirna mrna % sirna 5nM Silencer Select Pre-designed sirna Silencer Select Pre-designed sirna 2 70% RNA Silencer Select Validated sirna Silencer Select Validated sirna 80% mrna Silencer Select Pre-designed sirna 3 3 2 80% RNA 7. Silencer Select sirna Silencer Select Pre-designed Silencer Select Validated Custom Design Select sirna Custom Select sirna Silencer Select Negative Control #1 sirna Silencer Select Negative Control #2 sirna Silencer Select GAPDH Positive Control sirna Silencer Select Pre-designed sirna 98 Silencer Select Validated sirna RNA 80 RNA Silencer Select sirna sirna Select sirna 21 sirna Silencer Select sirna Silencer Select sirna sirna 2 Silencer Select GAPDH sirna GAPDH GAPDH sirna 22. / RNAi 33

sirna Silencer Select Pre-designed sirna Silencer Select Pre-designed sirna, inventoried, standard purity Silencer Select Pre-designed sirna, non-inventoried, standard purity Silencer Select Pre-designed sirna, HPLC purified *1 nmol 4 Silencer Select Validated sirna 1 nmol 4427037 13,700 5 nmol 4392420 45,200 20 nmol 4392421 69,300 40 nmol 4392422 87,200 5 nmol 4390771 45,200 20 nmol 4390815 69,300 40 nmol 4390816 87,200 20 nmol 4390817 100,800 40 nmol 4390818 118,700 Silencer Select Validated sirna, standard purity Silencer Select Validated sirna, HPLC purified *1 nmol 4 1 nmol 4427038 15,800 5 nmol 4390824 57,800 20 nmol 4390825 81,900 40 nmol 4390826 99,800 20 nmol 4390821 113,400 40 nmol 4390822 131,300 Silencer Select Negative Control sirna Silencer Select Negative Control #1 sirna Silencer Select Negative Control #2 sirna 5 nmol 4390843 31,500 40 nmol 4390844 94,500 250 nmol 4404020 294,000 5 nmol 4390846 31,500 40 nmol 4390847 94,500 Silencer Select GAPDH Positive Control sirna Silencer Select GAPDH sirna (Hs, Mm, Rn) Custom Select sirna 5 nmol 4390849 31,500 40 nmol 4390850 94,500 250 nmol 4404024 294,000 Custom Select sirna, standard purity Custom Select sirna, HPLC purified 5 nmol 4390827 47,300 20 nmol 4390828 71,400 40 nmol 4390829 89,300 20 nmol 4390830 102,900 40 nmol 4390831 120,800 sirna sirna 3 sirna sirna Silencer Select Human Kinase sirna Library V4 2,130 710 4397918 Silencer Select Human 894 298 4397919 Phosphatase sirna Library V4 Silencer Select Human 1,140 380 4397916 GPCR sirna Library V4 Silencer Select Human Nuclear 141 47 4397914 Hormone Receptor sirna Library V4 Silencer Select Human Ion 1,014 338 4397915 Channel sirna Library V4 Silencer Select Human Protease sirna Library V4 1,482 494 4397917 Silencer Select Human Druggable 27,096 9,032 4397920 Genome sirna Library V4-96-well Silencer Select Human Druggable 4,149 1,383 4397921 Genome V4 sirna Ext Set - 96-well Silencer Select Human Extended 31,245 10,415 4397927 Druggable Genome sirna Library V4-96-well Silencer Select Human Druggable 27,096 9,032 4397922 Genome sirna Library V4-384-well Silencer Select Human Druggable 4,149 1,383 4397924 Genome V4 sirna Ext Set - 384-well Silencer Select Human Genome 33,510 11,170 4397923 sirna V4 Extension Set - 384-well Silencer Select Human Genome sirna Library V4-384-well 64,755 21,585 4397926 0.25 nmol 0.5 nmol sirna sirna 36 sirna sirna sirna 36 0.1 nmol, 0.25 nmol, 1 nmol, 2 nmol 96 384 sirna Inventoried sirna non- Inventoried sirna 34

Ambion In Vivo sirna Invitrogen Ambion In Vivo sirna in vivo RNAi sirna Silencer Select Silencer Select 33 in vivo RNAi LNA 90% RNAi sirna remaining intact (%) Unmodified sirna: time (h) t 1/2 5 min Ambion In Vivo sirna: t 1/2 24 h Ambion In Vivo Pre-designed sirna, Standard Purity Ambion In Vivo Pre-designed sirna,hplc Ambion In Vivo Pre-designed sirna, HPLC In Vivo Ready Ambion In Vivo Custom sirna Standard Purity Ambion In Vivo Custom sirna, HPLC Ambion In Vivo Custom sirna, HPLC In Vivo Ready Ambion In Vivo Custom Designed Standard Purity Ambion In Vivo GAPDH Positive Control sirna Ambion In Vivo GAPDH Positive Control sirna, HPLC In Vivo Ready Ambion In Vivo Factor VII sirna, HPLC In Vivo Ready, 50 nmol Ambion In Vivo Factor VII Positive Control sirna, HPLC In Vivo Ready, 250 nmol Ambion In Vivo Negative Control #1 sirna, 5 nmol Ambion In Vivo Negative Control #1 sirna, HPLC In Vivo Ready, 50 nmol Ambion In Vivo Negative Control #1 sirna, HPLC In Vivo Ready, 250 nmol 5 nmol 4457298 47,300 250 nmol 4457302 315,000 1 µmol 4457304 504,000 250 nmol 4457308 336,000 1 µmol 4457310 525,000 5 nmol 4457297 47,300 250 nmol 4457301 337,100 1 µmol 4457303 539,300 250 nmol 4457307 336,000 1 µmol 4457309 525,000 5 nmol 4457296 47,300 5 nmol 4457288 39,900 50 nmol 4459407 126,000 250 nmol 4457291 336,000 50 nmol 4459408 126,000 250 nmol 4457292 336,000 5 nmol 4457287 39,900 50 nmol 4459405 126,000 250 nmol 4457289 336,000 23. sirna 100 8 Ambion In Vivo sirna 8 sirna 90% sirna HPLC TaqMan sirna Assays Applied Biosystems TaqMan sirna Assays Silencer Select sirna in vivo sirna TaqMan Assays sirna shrna TaqMan sirna Assays, 150 4440878 43,900 Made-to-Order, S TaqMan sirna Assays, 750 4440879 88,500 Made-to-Order, M TaqMan sirna Assays, 2,900 4440880 164,600 Made-to-Order, L *20 µl 35

RNA sirna mirna Lipofectamine RNAiMAX Invitrogen Lipofectamine RNAiMAX sirna mirna Silencer Select sirna 33 mirvana mirna mimics & inhibitors 43 sirna Invivofectamine 3.0 Reagent Invitrogen Invivofectamine 3.0 Reagent in vivornai sirna 85 sirna 85% sirna 90% p53 発現レベル細胞生存率 (%) p53 発現レベル細胞生存率 (%) 24. Lipofectamine RNAiMAX Lipofectamine RNAiMAX 試薬量 0.1 1.0 µl RNAiMAX p53 26. Invivofectamine 3.0 Reagent Factor VII PPIB sirnai mrna 85 p53, relative to nontransfected 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0 10 nm + Lipofectamine RNAiMAX Scrambled control 10 nm + B 社製品 10 nm + T 社製品 p53 sirna 10 nm + Q 社製品 1 nm + Lipofectamine RNAiMAX 1 nm + B 社製品 1 nm + T 社製品 1 nm + Q 社製品 Nontransfected 25. Lipofectamine RNAiMAX 27. sirna Invivofectamine 3.0 Reagent FVII sirna 0.02 2 mg/kg FVII sirna Invivofectamine 3.0 Reagent ED 50 0.1 mg/kg 10 1 1.0 mg/kg Lipofectamine RNAiMAX 0.1 ml 13778-100 10,200 0.3 ml 13778-030 29,500 0.75 ml 13778-075 71,300 1.5 ml 13778-150 101,300 15 ml 13778-500 831,300 Invivofectamine 3.0 Reagent 10 IVF3001 158,400 50 IVF3005 588,000 36

microrna microrna microrna mirna pol precursor mirna mirna 21-23nt mature mirna mirna non-coding RNA mirna mirna mrna 3'UTR mrna mirna Human Precursor mirna Mature mirna 28. mirna (Mature mirna) mirna mirna mirna mirbase www.mirbase.org 29. mirna 8. mirna mirna mirna mirna mirvana mirna Isolation Kit mirvana PARIS Kit TaqMan mirna ABC Purlfication Kit TaqMan MicroRNA Cells-to-CT Kit TaqMan Array MicroRNA Cards TaqMan Universal PCR Master Mix II Megaplex Primer Pools TaqMan MicroRNA RT Kit TaqMan MicroRNA Assays TaqMan Universal PCR Master Mix II TaqMan MicroRNA RT Kit mirvana mirna mimic mirvana mirna inhibitor Lipofectamine RNAiMAX TaqMan Gene Expression Cells-to-CT Kit TaqMan Gene Expression Assays 37

microrna mirvana mirna Isolation Kit RNA RNA microrna mirna small RNA Invitrogen mirvana mirna Isolation Kit mirna RNA mirna Total RNA 250mg Total RNA 30 mirvana mirna Isolation Kit 40 AM1560 57,000 mirvana mirna Isolation Kit without Phenol mirvana PARIS Kit 40 AM1561 49,800 small RNA RNA 2 1 Small RNA RNA mirna 30 small RNA RNA mrna mirna sirna 10 2 10 7 100mg 200 small RNA TaqMan mirna ABC Anti-miRNA Bead Capture Purification Kit mirna 75 Human Panel A B 754 mirna mirna Anti-miRNA oligos Dynabeads mirna mirna 10µL 50µL RT-qPCR TRIzol 75 mirna Avg. C t 40.00 35.00 30.00 25.00 20.00 15.00 10.00 cel-mir-39 expression profile serum sample (50 µl) hsa-mir-16 hsa-let-7a Assay ID 30. TaqMan mirna ABC Purification Ki 50µL hsa-mir-150 hsa-let-7c hsa-mir-135b RNA mirvana PARIS Kit 40 AM1556 65,500 31. ABC Purification Kit TaqMan mirna ABC Purification 40 4473087 67,500 Kit-Human Panel A TaqMan mirna ABC Purification Kit-Human Panel B 40 4473088 67,500 38

microrna TaqMan MicroRNA RT Kit Applied Biosystems TaqMan MicroRNA RT Kit TaqMan MicroRNA Assays 40 TaqMan MicroRNA Assays RT TaqMan MicroRNA RT Kit 200 4366596 27,300 Megaplex Primer Pools 1,000 4366597 81,800 Applied Biosystems Megaplex RT Primers stem-looped Sanger mirbase mirna mirna RNA Megaplex Primer Pools TaqMan MicroRNA Assays TaqMan MicroRNA Arrays Megaplex PreAmp Primers 1ng RNA Megaplex RT Primers Pool Rodent Megaplex RT Primers, Rodent Pool A 50 4399970 97,600 Megaplex RT Primers, Rodent Pool B v3.0 50 4444292 97,600 Megaplex RT Primers, Rodent Pool Set v3.0 RT Primers Pools A&B 4399970 & 4444292 50 4444746 112,400 Megaplex PreAmp Primers Pool Rodent Megaplex PreAmp Primers, 50 4399203 97,600 Rodent Pool A PreAmp Megaplex PreAmp Primers, 50 4444308 97,600 Rodent Pool B v3.0 PreAmp Megaplex PreAmp Primers, Rodent Pool Set v3.0 PreAmp Primers Pools A&B 4399203 & 4444308 50 4444747 112,400 Megaplex RT and PreAmp Primers Package Rodent Megaplex Primer Pools, 50 4401090 136,300 Rodent Pools A RT Primers 4399970 PreAmp Primers 4399203 Megaplex Primer Pools, 50 4444752 136,300 Rodent Pools B v3.0 RT Primers 4444292 PreAmp Primers 4444308 Megaplex Primer Pools, Rodent Pools Set v3.0 RT Primers Pools A&B 4444746 PreAmp Primers Pools A&B 4444747 50 4444766 154,500 Megaplex RT Primers Pool Human Megaplex RT Primers, Human Pool A 50 4399966 97,600 Megaplex RT Primers, Human Pool B v3.0 50 4444281 97,600 Megaplex RT Primers, Human Pool Set v3.0 RT Primers Pools A&B 4399966 & 4444281 50 4444745 112,400 Megaplex PreAmp Primers Pool Human Megaplex PreAmp Primers, 50 4399233 97,600 Human Pool A PreAmp Megaplex PreAmp Primers, 50 4444303 97,600 Human Pool B v3.0 PreAmp Megaplex PreAmp Primers, Human Pool Set v3.0 PreAmp Primers Pools A&B 4399233 & 4444303 50 4444748 112,400 Megaplex RT and PreAmp Primers Package Human Megaplex Primer Pools, 50 4401009 136,300 Human Pools A RT Primers 4399966 PreAmp Primers 4399233 Megaplex Primer Pools, 50 4444749 136,300 Human Pools B v3.0 RT Primers 4444281 PreAmp Primers 4444303 Megaplex Primer Pools, Human Pools Set v3.0 RT Primers Pools A&B 4444745 PreAmp Primers Pools A&B 4444748 50 4444750 154,500 TaqMan MicroRNA Arrays A B Megaplex Primer Pools 39

microrna TaqMan MicroRNA Assays Applied Biosystems TaqMan MicroRNA Assays PCR microrna mirna TaqMan MicroRNA Assays mirna mirna mirna mirna Assays Synthetic mirna Target Relative Detection (%) No. of base differences TaqMan mirna mirna PCR 3 1-10ng RNA 7 TaqMan MicroRNA Assays TaqMan MicroRNA Assays mirna loop mirna RT mirna mirna 5' TaqMan PCR mirna Mature mirna Looped RT primer 33. 1 TaqMan MicroRNA Assays 1 let-7a let-7a let-7c 3.7 TaqMan MicroRNA Assays, Inventoried* mirna RT primer 5X reaction mix (50 / 15 µl ) mirna TaqMan Assay 20X reaction mix (150 / 20 µl ) 50 150 4427975 33,500 TaqMan MicroRNA Assays, Made-to-Order, Small mirna RT primer 5X reaction mix (50 / 15 µl ) mirna TaqMan Assay 20X reaction mix (150 / 20 µl ) TaqMan MicroRNA Assays, Made-to-Order, Medium TaqMan MicroRNA Assays, Made-to-Order, Large 50 150 750 750 2,900 2,900 4440886 43,900 4440887 88,500 4440888 164,600 * Arabidopsis thaliana Caenorhabditis elegans Drosophila melanogaster Made-to-Order Sanger Release 16 mirna 2011 2 Custom TaqMan Small RNA Assays Forward primer TaqMan probe Reverse primer 32. TaqMan MicroRNA Assays TaqMan MicroRNA Assays mirna mirnas Custom TaqMan Small RNA Assays, 50 150 4398987 56,200 Custom TaqMan Small RNA Assays, Custom TaqMan Small RNA Assays, *20 µl 750 750 2,900 2,900 4398988 136,300 4398989 288,300 40

TaqMan Array MicroRNA Card Applied Biosystems TaqMan Array MicroRNA Card TaqMan 384 TaqMan 8 381 mirna 1 Megaplex RT Primers 39 TaqMan MicroRNA Array v2.0 / v3.0 Megaplex PreAmp Primers TaqMan MicroRNA Arrays v2.0 / v3.0 Megaplex Primer Pools Sanger mirbase 5 TaqMan Array Applied Biosystems 7900HT ViiA 7 QuantStudio TaqMan Array Human TaqMan Array Human MicroRNA A Cards v2.0 TaqMan Array Human MicroRNA A+B Cards Set v3.0 A array (4398965) + B Array (4444910) TaqMan Array Human MicroRNA B Cards v3.0 Rodent 4 4398965 227,000 8 4 4444913 292,800 4 4444910 227,000 TaqMan Array Rodent MicroRNA A Cards v2.0 TaqMan Array Rodent MicroRNA A+B Cards Set v3.0 A array (4398967) + B Array (4444899) TaqMan Array Rodent MicroRNA B Cards v3.0 8 4 4 4398967 227,000 4444909 292,800 4 4444899 227,000 mature mirna mirvana mirna Isolation Kit TaqMan mirna ABC Purification Kit Megaplex RT Primers & TaqMan MicroRNA Reverse Transcription Kit Megaplex PreAmp Primers & TaqMan PreAmp Master Mix Diluted RT product & TaqMan Universal PCR Master Mix II Applied Biosystems QuantStudio 12K Flex ViiA 7 / 7900 HT Fast PCR RT-PCR Expression Suite Software 34. mirna 41

Non-coding RNA TaqMan Pri-miRNA Assays mirna TaqMan Pri-miRNA Assays Applied Biosystems TaqMan Pri-miRNA Assays microrna mirna primary microrna Pri-miRNA microrna Pri-miRNA 1 microrna Drosha mirna mirna stem-loop Dicer RNA Induced Silencing Complex RISC mirna 35. microrna 37. hsa-mir-26 mir-26a-1 mir-26a-2 Pri-miRNA Stemloop Pri-miR-26a-2 TaqMan Pri-miRNA Assay hsa-mir-26a-2 TaqMan Non-coding RNA Assays TaqMan Non-coding RNA Assays Applied Biosystems TaqMan Non-coding RNA Assays non-cording TaqMan Gene Expression Assays TaqMan Non-coding RNA TaqMan Non-coding RNA Assays TaqMan PreAmp Master Mix Pre-Amplification 36. TaqMan Pri-miRNA Assays Sanger mirbase Stem-loop 500 Pri-miRNA Sanger mirbase sequence repository stem-loop mirna TaqMan Pri-miRNA Assay TaqMan MicroRNA Assay mirna TaqMan Non-coding RNA Assays, S 360 4426961 43,900 TaqMan Non-coding RNA Assays, M 750 4426962 88,500 TaqMan Non-coding RNA Assays, L 2,900 4426963 164,600 *20 µl TaqMan Pri-miRNA Assays, S 360 4427012 43,900 TaqMan Pri-miRNA Assays, M 750 4427013 88,500 TaqMan Pri-miRNA Assays, L 2,900 4427014 164,600 *20 µl 42

microrna mirvana mirna mimic & inhibitors mirna mirna mirvana mirna inhibitor Invitrogen mirvana mirna inhibitor mirna mirna mirna 1 mirbase in vivo in vivo ready mirvana mirna mimic Invitrogen mirvana mirna mimic mirna mirna mirna mirna 2 mirna mirvana mirna mimic 5 nmol 4464066 60,900 mirvana mirna mimic, in vivo Ready Customer-Defined mirvana mirna mimic Customer-Defined mirvana mirna mimic, in vivo Ready mirvana mirna mimic Negative Control #1 mirvana mirna mimic Negative Control #1, in vivo Ready mirvana mirna mimic mir-1 Positive Control mirvana mirna mimic mir-1 Positive Control, in vivo Ready mirvana mirna mimic mirbase v 20 2015 6 20 nmol 4464067 186,900 250 nmol 4464070 535,500 5 nmol 4464068 102,900 20 nmol 4464069 189,000 250 nmol 4464071 535,500 5 nmol 4464058 59,900 20 nmol 4464059 182,700 50 nmol 4464060 262,500 250 nmol 4464061 525,000 5 nmol 4464062 59,900 20 nmol 4464063 182,700 50 nmol 4464064 262,500 250 nmol 4464065 525,000 0.25 nmol each 1 nmol each 4464074 4464075 mirvana mirna inhibitor 5 nmol 4464084 60,900 20 nmol 4464085 186,900 mirvana mirna inhibitor, 250 nmol 4464088 535,500 in vivo Ready Customer-Defined mirvana 5 nmol 4464086 102,900 mirna inhibitor 20 nmol 4464087 189,000 Customer-Defined mirvana 250 nmol 4464089 535,500 mirna inhibitor, in vivo ready mirvana mirna inhibitor 5 nmol 4464076 59,900 Negative Control #1 20 nmol 4464077 182,700 mirvana mirna inhibitor 50 nmol 4464078 262,500 Negative Control #1, in vivo Ready 250 nmol 4464079 525,000 mirvana mirna inhibitor let-7 5 nmol 4464080 59,900 Positive Control 20 nmol 4464081 182,700 mirvana mirna inhibitor mirbase v 20 2015 6 Pre-miR mirna Precursor Molecule Pre-miR mirna Precursor 1 AM1540 98,700 Starter Kit Pre-miR mirna Precursors 5 nmol AM17100 57,800 Molecule 20 nmol AM17101 214,200 4 5 nmol Customer-Defined Pre-miR 20 nmol AM17103 214,200 mirna Precursor 4 5 nmol Pre-miR Negative Control #1 5 nmol AM17110 57,800 Pre-miR Negative Control #2 5 nmol AM17111 57,800 Cy3 Pre-miR Negative Control 5 nmol AM17120 65,100 FAM Pre-miR Negative Control 5 nmol AM17121 59,900 Pre-miR hsa-mir-1 mirna Precursor 5 nmol AM17150 57,800 Anti-miR mirna Inhibitor Anti-miR mirna Inhibitor 5 nmol AM17000 57,800 20 nmol 4 5 nmol AM17001 214,200 Customer Defined Anti-miR mirna Inhibitor 0.25 nmol each 1 nmol each 20 nmol 4 5 nmol 4464092 4464093 AM17003 214,200 Anti-miR Negative Control #1 5 nmol AM17010 57,800 Cy3 Anti-miR Negative Control 5 nmol AM17011 63,000 FAM Anti-miR Negative Control 5 nmol AM17012 59,900 mirna 36 Lipofectamine RNAiMAX Invivofectamine 3.0 43

SNP TaqMan SNP 2 TaqMan TaqMan 5' VIC dye FAM dye 3' Q PCR DNA VIC dye FAM dye TaqMan SNP Genotyping Assay 100 DNA 38. TaqMan SNP 39. Allele1 TaqMan VIC Allele2 TaqMan FAM VIC FAM Allele1 Allele2 Allele1 2 44

SNP TaqMan Pre-Designed SNP Genotyping Assays Applied Biosystems TaqMan SNP Genotyping Assays SNP SNP 450 1 SNP ID SNP csnp 7 SNP 16 SNP 350 SNP 2600 Human TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Small scale 300 1,500 4351379 59,600 TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Medium scale TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Large scale * 25 µl 5 µl non-human 1,000 5,000 2,400 12,000 4351376 107,900 4351374 208,900 TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Small scale 300 1,500 4351384 59,600 TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Medium scale TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Large scale * 25 µl 5 µl 1,000 5,000 2,400 12,000 4351382 107,900 4351380 208,900 Custom TaqMan SNP Genotyping Assays Applied Biosystems Custom TaqMan SNP Genotyping Assays SNP SNP SNP SNP SNP 6 MNP SNP Human Custom TaqMan SNP Genotyping 300 4331349 65,900 Assays, S-scale, Human 1,500 Custom TaqMan SNP Genotyping Assays, M-scale, Human Custom TaqMan SNP Genotyping Assays, L-scale, Human * 25 µl 5 µl non-human 1,000 5,000 2,400 12,000 4332072 124,900 4332073 249,700 Custom TaqMan SNP Genotyping 300 4332077 65,900 Assays, S-scale, non-human 1,500 Custom TaqMan SNP Genotyping Assays, M-scale, non-human Custom TaqMan SNP Genotyping Assays, L-scale, non-human * 25 µl 5 µl 1,000 5,000 2,400 12,000 4332075 124,900 4332076 249,700 TaqMan Drug Metabolism Genotyping Assays 220 2,000 TaqMan Drug Metabolism 150 4362691 67,000 Genotyping Assays 750 * 25 µl 5 µl 45

SNP SNP TaqMan Genotyping Master Mix Applied Biosystems TaqMan Genotyping Master Mix PCR GC AT TaqMan Universal Master Mix GC AT 72 Fast SNP TaqMan GTXpress Master Mix Applied Biosystems TaqMan GTXpress Master Mix Fast PCR SNP SNP TaqMan PCR 50 PCR 50 Tracking Dye 9. PCR 40. TaqMan Genotyping Master Mix GC-rich TaqMan Genotyping Master Mix 94 gdna TaqMan SNP Assay C 12050942_10 PCR GeneAmp PCR System 9700 7900HT PCR GC-rich Catjespin D rs12214 dbsnp TaqMan Genotyping Master Mix TaqMan Genotyping Master Mix, 80 4371353 15,800 1 ml 400 TaqMan Genotyping Master Mix, 10 ml TaqMan Genotyping Master Mix, 10 ml 2 TaqMan Genotyping Master Mix, 50 ml TaqMan Genotyping Master Mix, 50 ml 2 TaqMan Genotyping Master Mix TaqMan Genotyping Master Mix 800 4,000 1,600 8,000 4,000 20,000 8,000 40,000 4371355 129,300 4381656 247,200 4371357 561,800 4381657 1,089,800 1 4371131 1 4371130 TaqMan GTXpress Master Mix TaqMan GTXpress Master Mix, 1 ml 80 200 400 4403311 15,800 TaqMan GTXpress Master Mix, 10 ml TaqMan GTXpress Master Mix, 50 ml TaqMan GTXpress Master Mix, 100 ml 2 50 ml *PCR 96 Standard Block,0.2 µl 25 µl 96 Fast Block,0.1 µl 10 µl 384 5 µl 800 2,000 4,000 4,000 10,000 20,000 8,000 20,000 40,000 4401892 129,300 4401890 561,800 4401857 1,089,800 * 25 µl 5 µl 46

SNP TaqMan Sample-to-SNP Kit Applied Biosystems TaqMan Sample-to-SNP Kit DNA TaqMan SNP 1 DNA Fast PCR TaqMan GTXpress Master Mix DNA 5 PCR 50 SNP 1 41. TaqMan Sample-to-SNP Kit / FTA / / / Buccal swab / Rat Mouse tail / Leaf punch / / FFPE DNA 95 3 DNA PCR A. Buccal swab, B. Mouse tail, C. Corn leaf, D. FFPE Fast PCR TaqMan GTXpress Master Mix PCR 50 TaqMan Sample-to-SNP Kit 5 ml sample prep, 1 ml PCR TaqMan Sample-to-SNP Kit 20 ml sample prep, 10 ml PCR TaqMan Sample-to-SNP Kit 20 ml sample prep, 50 ml PCR TaqMan Sample-to-SNP Kit 200 ml sample prep, 10 ml PCR * PCR 25 µl DNA 100 PCR 80 DNA 400 PCR 800 DNA 400 PCR 4,000 DNA 4,000 PCR 800 4403313 38,200 4403083 202,300 4403087 618,000 4403081 629,200 42. TaqMan Sample-to-SNP Kit SNP www.lifetechnologies.com/taqmanjp SNP / 47

TaqMan Mutation Detection Assays Applied Biosystems TaqMan Mutation Detection Assays Competitive Allele-Specific TaqMan PCR castpcr MGB 0.1 1 3 1 10. TaqMan Mutation Detection Assay 11. アッセイのタイプ説明模式図 Reference Assay Mutant Assay Mutant Allele Assay 特定の変異型アレルを検出します (EGFR_exlqdels_mu* は 19 種類の変異を検出 ) アレル特異的プライマーが変異型アレルを検出します MGB ブロッカーオリゴスクレオチドが野生型アレルを抑えます ASP = 対立遺伝子特異的プライマー ASB = 対立遺伝子特異的ブロッカー (MGB) LST = 遺伝子特異的 TaqMan プローブ LSP = 遺伝子特異的プライマー EGFR exon19 deletion Wild type Assay Mutant Assay EGFR Reference Assay EGFR Exon19 Deletion Assay Wild type Allele Assay 野生型アレルを検出しますアレル特異的プライマーが野生型アレルを検出します MGB ブロッカーオリゴヌクレオチドが変異型アレルを抑えます ASP = 対立遺伝子特異的プライマー ASB = 対立遺伝子特異的ブロッカー (MGB) LST = 遺伝子特異的 TaqMan プローブ LSP = 遺伝子特異的プライマー Mutation Detector TaqMan Mutation Detection Assay Gene Reference Assay 変異を含む遺伝子を検出します遺伝子特異的なプライマーペアで遺伝子内の変異のない領域を増幅します FP = フォワードプライマー RP = リバースプライマー LST = 遺伝子特異的 TaqMan プローブ ASP: LST: TaqMan LSP: MGB: ASB: MGB FP: RP: TaqMan Mutation Detection Assays 46 778 ** 150 µl 75 4465804 90,800 TaqMan EGFR Exon 19 Deletions Assay 1 EGFR 19 1 ** 150 µl 75 4465805 90,800 TaqMan Mutation Detection Reference Assays 46 ** 150 µl 75 4465807 56,200 TaqMan Mutation Detection IPC Reagent Kit 1 kit 500 4467538 31,800 *20 µl ** 2015 6 www.lifetechnologies.com TaqMan Mutation Detection Assays 48

TaqMan Copy Number Assays Copy Number Variations, CNVs CNVs CNVs Applied Biosystems TaqMan Copy Number Assays CNVs 160 TaqMan CNVs TaqMan Copy Number Assays TaqMan Copy Number Reference Assays PCR 3 4 PCR DNA 3 4 160 Copy Number 4 TaqMan Copy Number 360 assays 4400291 50,700 Assays, Small 720 assays TaqMan Copy Number Assays, Medium TaqMan Copy Number Assays, Large 750 assays 1,500 assays 2,900 assays 5,800 assays 4400292 96,600 4400293 181,600 * 20 µl 96 10 µl 384 Custom TaqMan Copy Number Assays Applied Biosystems Custom TaqMan Copy Number Assays TaqMan Copy Number Assays CNVs GeneAssist Copy Number Assay Tool CNVs Custom TaqMan Copy Number 360 assays 4400294 56,200 Assays, Small 720 assays Custom TaqMan Copy Number Assays, Medium Custom TaqMan Copy Number Assays, Large 750 assays 1,500 assays 2,900 assays 5,800 assays 4400295 110,200 4400296 204,300 * 20 µl 96 10 µl 384 CopyCaller TaqMan Copy Number Assays Applied Biosystems CopyCaller www.lifetechnologies.com/copycaller TaqMan Copy Number Reference Assays Applied Biosystems TaqMan Copy Number Reference Assays TaqMan Copy Number Assays Custom TaqMan Copy Number Assays RNase P Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2 TERT RNase P telomerase reverse transriptase (TERT) gene located on chromosome 5, cytoband 5p15.33 Human TaqMan Copy Number 750 assays 4403326 20,500 Reference Assay, RNase P 1,500 assays 3,000 assays 6,000 assays 4403328 57,900 TaqMan Copy Number Reference Assay, TERT 750 assays 1,500 assays 3,000 assays 6,000 assays 4403316 20,500 4403315 57,900 Mouse TaqMan Copy Number 750 assays 4458366 20,500 Reference Assay, TFRC 1,500 assays 3,000 assays 6,000 assays 4458367 57,900 TaqMan Copy Number Reference Assay, TERT 750 assays 1,500 assays 3,000 assays 6,000 assays 4458368 20,500 4458369 57,900 * 20 µl 96 10 µl 384 Custom Plus TaqMan Copy Number Assays Applied Biosystems Custom Plus TaqMan Copy Number Assays Custom TaqMan Copy Number Assays SNP TaqMan TaqMan Copy Number Assays Custom Plus TaqMan 360 assays 4442487 56,200 Copy Number Assays, Small 720 assays Custom Plus TaqMan Copy Number Assays, Medium Custom Plus TaqMan Copy Number Assays, Large 750 assays 1,500 assays 2,900 assays 5,800 assays 4442520 110,200 4442488 204,300 * 20 µl 96 10 µl 384 49

High Resolution Melt HRM High Resolution Melting HRM PCR PCR PCR Applied Biosystems MeltDoctor HRM MeltDoctor HRM ステップ 1: 装置のキャリブレーション * ステップ 2: PCR の準備ステップ 3: PCR ステップ 4: HRM 解析ステップ 5: データ解析 HRM の蛍光色素とプライマーの設計を行い PCR 増幅を行いますリアルタイム PCR で融解曲線のデータをバックグラウンドの必要に応じて PCR 条件のステップ 3 ~ 5 はリアルタイム融解曲線を描きます解析しますキャリブレーションを最適化を行います PCR を使った場合の一連の行います操作となります *Only required once every six months 43. HRM HRM PCR MeltDoctor HRM High Resolution Melting HRM PCR MeltDoctor HRM SYTO 9 DNA Tm PCR dutp dntp PCR HRM 50

MeltDoctor HRM HRM AmpliTaq Gold 360 DNA Polymerase PCR MeltDoctor HRM 2 SYTO 9 MeltDoctor HRM MeltDoctor HRM HRM PCR MeltDoctor HRM 1 500 units 4425557 50,400 AmpliTaq Gold 360 DNA Polymerase GeneAmp dntp Blend MeltDoctor HRM Dye MeltDoctor HRM 500 4415440 41,000 5 ml MeltDoctor HRM 2,500 4415452 189,000 5 5 ml MeltDoctor HRM 5,000 4415450 357,000 10 5 ml MeltDoctor HRM 5,000 4409535 336,000 50 ml *20 µl MeltDoctor HRM Applied Biosystems MeltDoctor HRM 3 AA, AB, BB 3 DNA MeltDoctor HRM 1 450 4410126 42,000 MeltDoctor HRM Applied Biosystems MeltDoctor HRM 96 Fast 384 2 MeltDoctor HRM DNA 44. HRM 3 HeLa, Raji, and Jurkat HRM p53 4 152bp PCR 3 HRM GC, GG, CC HRM MeltDoctor HRM Applied Biosystems 7500 Fast PCR Applied Biosystems HRM Software v2.0 HRM PCR BigDye Terminator v1.1 Cycle Sequencing Kit Applied Biosystems 3130 MeltDoctor HRM 1 4425618 52,500 96 Fast MeltDoctor HRM 1 4425559 52,500 384 MeltDoctor HRM 20 1 ml 4425562 42,000 HRM HRM HRM PCR www.lifetechnologies.com/hrm 51

TaqMan Protein Assays 市販または自家調製抗体 SA- オリゴ A 3 末端オリゴアッセイプローブ A 5 末端オリゴ SA- オリゴ B アッセイプローブ B エピトープ 1 エピトープ 2 Build Your Assay 90 min Prepare Sample 30 min Binding 60 min - TaqMan Protein Assay Open Kit Protein Quant Sample Lysis Kit Protein Expression Sample Lysis Kit Ambion PARIS Kit TaqMan Protein Assay TaqMan Protein Assay Open Kit TaqMan Protein Assay Kit 高濃度中濃度低濃度コネクター蛍光強度バックグラウンド相対発現量変化サイクル数 Ligation/ Inactivation 25 min TaqMan Fast 40 min Real-Time PCR Data Analysis DNA TaqMan Protein Expression Core Reagents Kit with Master Mix Universal TaqMan Assay PCR TaqMan PCR mrna mirna ProteinAssist Software v1.0 45. TaqMan Protein Assays 52

TaqMan Protein Assays Applied Biosystems TaqMan Protein Assays TaqMan 5 mrna mirna 500 TaqMan Protein Assays Kit hesc Applied Biosystems TaqMan Protein Assays Kit hicam1 hcstb TaqMan Protein Assay Kit hcstb 100 4405465 63,600 TaqMan Protein Assay Kit hicam1 100 4405471 63,600 4 TaqMan Protein Expression Core Reagents Kit with Master Mix Applied Biosystems TaqMan Protein Expression Core Reagents Kit with Master Mix PCR TaqMan Protein Assays Kit TaqMan Protein Assays Open Kit TaqMan Protein Assay Core Reagent Base Kit DNA Ligase, Protease, Ligase PCR / TaqMan Protein Assays Fast Master Mix ph PCR TaqMan Protein Assay Open Kit Applied Biosystems TaqMan Protein Assay Open Kit TaqMan Protein Assay 2 TaqMan PCR mrna mirna TaqMan Protein Assays Open Kit 4,000 4453745 145,400 TaqMan Protein Assays Oligo Probe Kit TaqMan Protein Assays Buffer Kit TaqMan Protein Assays Oligo 4,000 4448549 61,400 Probe Kit 5'Prox-Oligo 200 nm, 50 µl 3'Prox-Oligo 200 nm, 50 µl TaqMan Protein Assays Buffer Kit Antibody Dilution Buffer, 1 ml Assay Probe Strage Buffer, 1 ml Assay Probe Dilution Buffer, 7 ml Lysate Dilution Buffer, 25 ml 4,000 4448571 94,300 TaqMan Protein Expression Core Reagents Kit with Master Mix 100 4405501 65,900 TaqMan Protein Expression Core Reagents Base Kit 4405460 TaqMan Protein Assays Fast Master Mix 4400088 TaqMan Protein Expression Core Reagents Kit with Master Mix 500 4448591 193,000 TaqMan ProteinExpression Core Reagents Base Kit 4448592 TaqMan Protein Assays Fast Master Mix 4448616 TaqMan Protein Assays 100 4405460 54,500 Core Reagents Base Kit 500 4448592 156,700 TaqMan Protein Assays 100 4400088 22,700 Fast Master Mix 500 4448616 63,600 53

Protein Expression Sample Preparation Kit TaqMan Protein Assay Kit TaqMan Protein Assay Open Kit 10,000 50,000 Protein Quant Sample Lysis Kit 50,000 12. Protein Expression Sample Preparation Kit Protein Quant Sample Lysis Kit 10,000 50,000 50,000 Lysis Yes No TaqMan Protein Assays 500 cells/µl 2,500 cells/µl TaqMan Protein Assay 1:2 1:10 Protein Expression Lysate Control Kits Applied Biosystems TaqMan Protein Expression Lysate Control Kits Protein Expression Lysate Control Kit Raji hcstb hicam1 Protein Expression Lysate Control Kit 100 4405448 68,100 Raji ProteinAssist Applied Biosystems TaqMan ProteinAssist ProteinAssist www.appliedbiosystems.com/proteinassist Protein Expression Sample Prep Kit 1,000 4405443 81,800 cell counting Protein Quant Sample Lysis Kit cell counting or total protein quant 25 ml 4448536 31,800 54

PCR Protein Thermal Shift Applied Biosystems Protein Thermal Shift PCR PCR 1 10 * PCR 48 96 384 * 1 Protein Thermal Shift 10 4466037 513,600 1.0 Protein Thermal Shift 1 4466038 256,800 Protein Thermal Shift 2,000 4461146 21,400 Dye Kit Protein Thermal Shift Starter Kit 2,000 + 100 4462263 49,500 100,000 Protein 80,000 Protein + Ligand d /dt 60,000 40,000 20,000 0 15,000 10,000 5,000 0-5,000 30.0 32.5 35.0 37.5 40.0 42.5 45.0 47.5 50.0 52.5 55.0 ~7oC Tm Difference 30.0 32.5 35.0 37.5 40.0 42.5 45.0 47.5 50.0 52.5 55.0 Temperature (oc) StepOne /StepOnePlus 7500 / 7500 Fast QuantStudio 7 / QuantStudio 12K Flex PCR 46. Tm 30 PCR 47. Protein Thermal Shift 55

13. ルシイ 384-Well Clear Optical Reaction Plate ( 製品番号 4309849) 96-Well Reaction Plate ( 製品番号 N8010560 / 4306737) + + Adhesive Film ( 製品番号 4311971 / 4360954) Adhesive Film ( 製品番号 4311971 / 4360954) QuantStudio 12K Flex リアルタイム PCR システム QuantStudio 12K Flex 96-Well Reaction Plate ( 製品番号 N8010560 / 4306737) Optical Tubes ( 製品番号 N8010933) or and + Optical Caps (Flat Cap) ( 製品番号 4323032) Optical 8-Tube Strip ( 製品番号 4316567) + + Tray / Retainer Set ( 製品番号 4381850) Optical Caps (Flat Cap) ( 製品番号 4323032) QuantStudio 7 Fast 8-Tube Strip, 0.1mL ( 製品番号 4358293) + 96-Well Tray for VeriFlex Blocks ( 製品番号 4379983) + Optical 8-Cap Strip ( 製品番号 4323032) Fast Reaction Tube with Cap, 0.1mL ( 製品番号 4358297) + 96-Well Tray for VeriFlex Blocks ( 製品番号 4379983) QuantStudio 7 リアルタイム PCR システム Fast 96-Well Reaction Plate ( 製品番号 4346907 / 4346906) + Adhesive Film ( 製品番号 4311971 / 4360954) QuantStudio 3 QuantStudio 5 (96 ウェル ) 96-Well Reaction Plate ( 製品番号 N8010560 / 4306737) + Optical 8-Tube Strip ( 製品番号 4316567) Adhesive Film ( 製品番号 4311971 / 4360954) + + Tray / Retainer Set ( 製品番号 4381850) Optical 8-Cap Strip ( 製品番号 4323032) QuantStudio 3 / QuantStudio 5 リアルタイム PCR システム QuantStudio 3 QuantStudio 5 (Fast 96 ウェル ) Fast 96-Well Reaction Plate ( 製品番号 4346907 / 4346906) + Fast 8-Tube Strip, 0.1mL ( 製品番号 4358293) Adhesive Film ( 製品番号 4311971 / 4360954) + + 96-Well Tray for VeriFlex Blocks ( 製品番号 4379983) Optical 8-Cap Strip ( 製品番号 4323032) QuantStudio 5 (384 ウェル ) 384-Well Clear Optical Reaction Plate ( 製品番号 4309849) + Adhesive Film ( 製品番号 4311971 / 4360954) 384-Well Clear Optical Reaction Plate ( 製品番号 4309849) + Adhesive Film ( 製品番号 4311971 / 4360954) Applied Biosystems 7900HT Fast リアルタイム PCR システム 7900HT 96-Well Reaction Plate ( 製品番号 N8010560 / 4306737) 96-Well Reaction Plate ( 製品番号 N8010560 / 4306737) + + Adhesive Film ( 製品番号 4311971 / 4360954) Optical 8-Cap Strip ( 製品番号 4323032) + Compression Pad ( 製品番号 4312639) Fast 96-Well Reaction Plate ( 製品番号 4346907 / 4346906) + Adhesive Film ( 製品番号 4311971 / 4360954) 7500 Fast Fast 96-Well Reaction Plate ( 製品番号 4346907 / 4346906) Fast 8-Tube Strip, 0.1mL ( 製品番号 4358293) + + Adhesive Film ( 製品番号 4311971 / 4360954) Optical 8-Cap Strip ( 製品番号 4323032) Applied Biosystems 7500 Fast / 7500 リアルタイム PCR システム 7500 96-Well Reaction Plate ( 製品番号 N8010560 / 4306737) + Adhesive Film ( 製品番号 4311971 / 4360954) 7300 Optical Tubes or Optical 8-Tube Strip Optical 8-Cap Strip 注 :Optical 8-Tube Strip を使用の ( 製品番号 N8010933) and ( 製品番号 4316567) + ( 製品番号 4323032) 場合には縦向きにセット Fast 96-Well Reaction Plate ( 製品番号 4346907 / 4346906) + Adhesive Film ( 製品番号 4311971 / 4360954) StepOne Plus Fast 8-Tube Strip, 0.1mL ( 製品番号 4358293) Fast Reaction Tube with Cap, 0.1mL ( 製品番号 4358297) + + 96-Well Tray for VeriFlex Blocks ( 製品番号 4379983) 96-Well Tray for VeriFlex Blocks ( 製品番号 4379983) + Optical 8-Cap Strip ( 製品番号 4323032) StepOnePlus / StepOne リアルタイム PCR システム StepOne Fast Optical 48-Well Reaction Plate ( 製品番号 4375816) Fast 8-Tube Strip, 0.1mL ( 製品番号 4358293) + + 48-Well Optical Adhesive Film ( 製品番号 4375928 / 4375323) Fast 48-Well Tray ( 製品番号 4375282) + Optical 8-Cap Strip ( 製品番号 4323032) Fast Reaction Tube with Cap, 0.1mL ( 製品番号 4358297) + Fast 48-Well Tray ( 製品番号 4375282) 56

Fast 96-Well 7900HT Fast 96 / 7500 Fast / StepOnePlus ViiA 7 / QuantStudio Fast 96 MicroAmp Fast 96-Well Reaction Plate 0.1 ml MicroAmp Fast Optical 96-Well Reaction Plate with Barcode 0.1 ml 96-Well 10 4346907 7,200 20 4346906 18,000 200 4366932 163,000 7900HT 96 / 7700 / 7500 / 7300 / 7000 / 5700 ViiA 7 / QuantStudio Standard 96 MicroAmp Optical 96-Well Reaction Plate 10 N8010560 7,200 MicroAmp Optical 96-Well Reaction Plate 20 4306737 17,800 with Barcode MicroAmp Optical 96-Well Reaction Plate with Barcode 500 4326659 381,900 MicroAmp Optical 96-Well Reaction Plate with Barcode 4306737 25 MicroAmp Optical 96-Well Reaction Plate with Barcode and Optical Caps 1 403012 29,700 MicroAmp Optical 96-Well Reaction Plate with Barcode 4306737 1 MicroAmp Optical 8-Cap Strip 4323032 1 384-Well 7900HT 384 / ViiA 7 / QuantStudio 384 MicroAmp Optical 384-Well Reaction 50 4309849 56,200 Plate with Barcode MicroAmp Optical 384-Well Reaction 500 4326270 449,300 Plate with Barcode MicroAmp Optical 384-Well Reaction Plate with Barcode 4309849 10 MicroAmp Optical 384-Well Reaction 1,000 4343814 741,400 Plate with Barcode Optical 384-Well Reaction Plate 1000 Code 128 MicroAmp Optical 384-Well Reaction Plate 1,000 4343370 718,900 Optical 384-Well Reaction Plate 1000 EnduraPlate 96-Well Fast MicroAmp EnduraPlate Optical 96-Well Fast Plate Clear 20 500 4483485 4483494 21,000 483,000 Multicolor Clear Yellow Blue Green Red 5 500 4483493 4483496 Endura 5,300 483,000 Yellow 20 4483495 21,000 Blue 20 4483486 21,000 Green 20 4483487 21,000 Red 20 4483492 21,000 96-Well MicroAmp EnduraPlate Optical 96-Well Clear Multicolor Clear Yellow Blue Green Red 20 500 5 500 4483354 4483352 4483355 4483356 31,500 410,100 8,000 410,100 Yellow 20 4483395 31,500 Blue 20 4483343 31,500 Green 20 4483349 31,500 Red 20 4483350 31,500 384-Well MicroAmp EnduraPlate Optical 384-Well Clear Multicolor Clear Yellow Blue Green Red 20 500 5 500 4483285 4483273 4483316 4483317 36,000 494,300 9,000 494,300 Yellow 20 4483315 36,000 Blue 20 4483320 36,000 Green 20 4483321 36,000 Red 20 4483322 36,000 57

7900HT / 7700 / 7500 / 7500 Fast / 7300 / 7000 / 5700 / StepOnePlus / ViiA 7 / QuantStudio MicroAmp Optical Adhesive Film 25 4360954 11,300 100 4311971 36,600 MicroAmp Adhesive Film Applicator 5 4333183 11,300 5 96-Well Compression Pad 7900HT* / 7700 / 7000 / 5700 96 Optical Adhesive Film+Compression Pad *Fast 96 384 TaqMan Low Density Array Compression Pad MicroAmp Optical Adhesive Film Kit 1 4313663 9,700 MicroAmp Optical Adhesive Film 20 1 MicroAmp Optical Film Compression Pad 1 7900HT 96 * / 7700 / 7500 / 7500Fast / 7000 / 5700 / StepOnePlus / StepOne / ViiA 7 / QuantStudio *384 TaqMan Array Optical Cap MicroAmp Optical 8-Cap Strip Flat 300 4323032 17,900 N8010935 7700 / 7500 / 7000 / 5700 / ViiA 7 / QuantStudio Standard 96 MicroAmp Optical 8-Tube Strip 0.2 ml MicroAmp Optical Tube without Cap 0.2 ml 125 4316567 17,900 2,000 N8010933 43,000 58

Fast 7500Fast / StepOnePlus / StepOne / ViiA 7 / QuantStudio Fast 96 MicroAmp Fast 8-Tube Strip 0.1 ml 125 4358293 20,400 & 7700 / 7000 / 5700 MicroAmp 96-Well Tray/Retainer Set 10 403081 13,700 Fast StepOnePlus / StepOne MicroAmp Fast Reaction Tube with Cap 0.1 ml 1,000 4358297 23,800 StepOne 48-Well StepOne 48 MicroAmp Fast Optical 48-Well 20 4375816 13,500 Reaction Plate MicroAmp Optical 48-Well Adhesive 25 4375928 11,100 Film 100 4375323 32,700 MicroAmp Fast 48-Well Trays 10 4375282 12,500 MicroAmp 48-Well Base Adaptor 5 4375284 6,700 96 96 *StepOne 4379590 MicroAmp Splash Free 96-Well Base 10 4312063 15,500 MicroAmp 96-Well Support Base 10 4379590* 15,600 StepOnePlus StepOnePlus 96 MicroAmp 96-Well Tray for VeriFlex Blocks ViiA 7 / QuantStudio 10 4379983 10,200 ViiA 7 / QuantStudio Standard 96 MicroAmp 96-Well Tray/Retainer Set for Veriti System 10 4381850 11,800 Compression Pad 7900HT* / 7700 / 7000 / 5700 96 Optical Adhesive Film Compression Pad *Fast 96 384 TaqMan Low Density Array Compression Pad MicroAmp Optical Film Compression Pad 5 4312639 8,600 ViiA 7 / QuantStudio Fast 96 MicroAmp 96-Well Tray for VeriFlex Blocks 10 4379983 10,200 LAMP for GeneAmp 5700 and ABI PRISM 7000 System LAMP for Applied Biosystems 7300/7500 Replacement Lamp for ViiA 7/ QuantiStudio 6 / QuantStudio 7 1 4309224 18,000 1 4345287 25,700 1 4443978 25,700 59

TaqMan Applied Biosystems TaqMan HPLC TaqMan MGB TaqMan 3' Tm Minor Groove Binder MGB TaqMan TAMRA Tm TaqMan MGB Probe Small size 6,000 pmol 4316034 69,700 TaqMan MGB Probe Medium size 20,000 pmol 4316033 145,400 TaqMan MGB Probe Large size 50,000 pmol 4316032 186,200 14. First Choice Second Choice DNA/RNA FAM VIC Minor Allele Major Allele SNP FAM VIC * FAM VIC TaqMan TAMRA TaqMan TAMRA Probe Small size 6,000 pmol 450025 58,500 TaqMan TAMRA Probe Medium size 20,000 pmol 450024 110,200 TaqMan TAMRA Probe Large size 50,000 pmol 450003 153,300 TaqMan MGB MGB TaqMan MGB Small size 4324034 73,100 6,000 pmol 1 27,000 pmol 2 TaqMan MGB Medium size 4324035 152,200 20,000 pmol 1 90,000 pmol 2 TaqMan MGB Large size 4324036 198,600 50,000 pmol 1 225,000 pmol 2 TaqMan TAMRA TAMRA TaqMan Probe 4323969 63,600 6,000 pmol 1 27,000 pmol 2 TaqMan TaqMan MGB SNP MGB 2 TaqMan MGB SNP Small size 4323297 134,900 6,000 pmol 2 27,000 pmol 2 TaqMan MGB SNP Medium size 4323298 283,700 20,000 pmol 2 90,000 pmol 2 TaqMan MGB SNP Large size 4323300 363,200 50,000 pmol 2 225,000 pmol 2 TaqMan QSY Applied Biosystems TaqMan QSY 4 3 MGB QSY TaqMan QSY 6,000 pmol 4482777 58,500 20,000 pmol 4482778 110,200 50,000 pmol 4482779 153,300 TaqMan TaqMan Universal Master Mix II TaqMan Gene Expression Master Mix TaqMan Universal PCR Master Mix TaqMan Fast Universal PCR Master Mix TaqMan Fast Advanced Master Mix SYBR Green Power SYBR Green PCR Master Mix Fast SYBR Green Master Mix SYBR Green PCR Master Mix SYBR Select Master Mix TaqMan SYBR Green * 250 nm 900 nm 50 nm 900 nm 50 nm * 2 60

TaqMan Assays sirna ID E www.lifetechnologies.com/taqman-order TaqMan Gene Expression Assays, Inventoried 24 TaqMan Gene Expression Assays, Made to Order 24 Custom TaqMan Gene Expression Assays 24 Custom Plus TaqMan RNA Assays 24 TaqMan Array Plate 25 TaqMan Custom Arrays 28 Silencer Select sirna 33 TaqMan sirna Assays 35 TaqMan Micro RNA Assays 40 Custom TaqMan Small RNA Arrays 40 TaqMan Pri-miRNA Assays 42 TaqMan Non-coding RNA Assays 42 mirvana mirna mimics 43 mirvana mirna Inhibitors 43 Pre-miR mirna Precursor Molecule 43 Anti-miR mirna Inhibitor 43 TaqMan SNP Genotyping Assays 45 Custom TaqMan SNP Genotyping Assays 45 TaqMan Drug Metabolism Genotyping Assays 45 TaqMan Mutation Detection Assays 48 TaqMan Copy Number Assays 49 Custom TaqMan Copy Number Assays 49 E ウェブ登録画面に必要事項を記入する Applied Biosystems 製品取扱代理店に登録用紙を渡す代理店が必要事項を記入し弊社宛に Email / FAX ユーザー ID とパスワードをご登録者にEメールでお知らせ E JP.Admin@lifetech.com 03-6832-6980 TaqMan MGB 60 TaqMan 60 61

TaqMan Custom Plating Service Applied Biosystems TaqMan Custom Plating Service TaqMan Assay 96 384 TaqMan Gene Expression Assays TaqMan MicroRNA Assays SNP Genotyping Assay Copy Number Assay Non coding RNA Assay sirna TaqMan Gene Expression Assays, Inventoried TaqMan Gene Expression Assays, Made-to-order TaqMan MicroRNA Assays, Inventoried TaqMan MicroRNA Assays, Made-to-order TaqMan Non-coding RNA Assays, Inventoried TaqMan Non-coding RNA Assays, Made-to-order TaqMan SNP Genotyping Assays DME TaqMan Copy Number Assays 96 Standard 96 Fast 384 10 µl 384 20 µl 96 20 62

INDEX -1-4311235 17 4331182 24,30 4351891 18 10296010 10 4311971 58 4331348 24 4352042 18 10296028 10 4312063 59 4331349 45 4352338E 31 11754050 16 4312639 59 4332072 45 4352339E 31 11754250 16 4312660 17 4332073 45 4352340E 31 11755050 16 4312704 23 4332075 45 4352341E 31 11755250 16 4313663 58 4332076 45 4352405 21 11755550 16 4316032 60 4332077 9,45 4352930E 30,31 12183016 10 4316033 60 4332078 24 4352931E 31 12183018A 10 4316034 60 4332079 24 4352932E 31 12183020 10 4316567 58 4333183 58 4352933E 31 12183025 10 4318157 20 4334973 23 4352934E 30 12183026 10 4319413E 30,31 4342247 28 4352935E 30 12183555 10 4323032 58 4342249 28 4358293 59 12185010 10 4323297 60 4342253 28 4358297 59 13778-030 36 4323298 60 4342259 28 4360954 58 13778-075 36 4323300 60 4342261 28 4362691 45 13778-100 36 4323969 60 4342265 28 4364103 18 13778-150 36 4324018 20 4343370 57 4364338 20 13778-500 36 4324020 20 4343814 57 4364340 20 15596018 10 4324034 60 4344463 23 4364341 20 15596018SP 10 4324035 60 4345287 59 4364343 20 15596026 10 4324036 60 4346798 28 4366072 18-4- 4326270 57 4346799 28 4366073 18 402929 17 4326314E 30 4346800 28 4366596 39 403012 57 4326315E 30 4346802 28 4366597 39 403081 59 4326316E 30 4346906 57 4366932 57 4304134 17 4326317E 30 4346907 57 4367218 23 4304437 20 4326318E 30 4351368 24 4367563 28,29 4304449 20 4326319E 30 4351370 24 4367659 23 4305719 20 4326320E 30 4351372 24 4367660 23 4306737 57 4326321E 30 4351374 45 4367784 28 4307281 17 4326322E 30 4351376 45 4367785 28 4309155 23 4326323E 30 4351379 45 4367786 28 4309224 59 4326614 20 4351380 45 4367846 18 4309849 57 4326659 57 4351382 45 4368577 23 4310251 23 4326708 20 4351384 45 4368702 23 63

INDEX 4368706 23 4378713 28 4390829 34 4397921 34 4368708 23 4378714 28 4390830 34 4397922 34 4368813 17 4379590 59 4390831 34 4397923 34 4368814 17 4379961 28 4390843 34 4397924 34 4369016 20 4379983 59 4390844 34 4397926 34 4369510 20 4381656 46 4390846 34 4397927 34 4369514 20 4381657 46 4390847 34 4398965 28,41 4369542 20 4381850 59 4390849 34 4398967 28,41 4370048 20 4383547 9 4390850 34 4398987 40 4370074 20 4384267 21 4391003 15 4398988 40 4370573 28 4385344 28 4391112 15 4398989 40 4371130 46 4385363 28 4391128 21 4399002 13 4371131 46 4385371 23 4391178 15 4399003 13 4371134 20 4385372 23 4391524 25 4399203 39 4371135 20 4385610 23 4391525 25 4399233 39 4371353 46 4385612 23 4391526 25 4399966 39 4371355 46 4385614 23 4391527 25 4399970 39 4371357 46 4385616 23 4391528 25 4400088 53 4374966 17 4385617 23 4391529 25 4400291 49 4374967 17 4385618 23 4391848 13 4400292 49 4375282 59 4386995 13 4392339 17 4400293 49 4375284 59 4387299 13 4392420 34 4400294 49 4375323 59 4387406 17 4392421 34 4400295 49 4375575 17 4387951 17 4392422 34 4400296 49 4375816 59 4389986 15 4392653 15 4401009 39 4375928 59 4390771 34 4392656 15 4401090 39 4378700 28 4390815 34 4392938 15 4401857 46 4378701 28 4390816 34 4393463 15 4401890 46 4378702 28 4390817 34 4393464 15 4401892 46 4378703 28 4390818 34 4396840 27,29 4402953 13 4378704 28 4390821 34 4397914 34 4402954 13 4378705 28 4390822 34 4397915 34 4402955 13 4378706 28 4390824 34 4397916 34 4402956 13 4378707 28 4390825 34 4397917 34 4402957 13 4378708 28 4390826 34 4397918 34 4402959 13 4378709 28 4390827 34 4397919 34 4402960 13 4378710 28 4390828 34 4397920 34 4403081 47 64

INDEX 4403083 47 4414077 26 4414114 26 4414151 27 4403087 47 4414078 26 4414115 26 4414152 27 4403311 46 4414079 26 4414116 26 4414153 27 4403313 47 4414080 26 4414117 26 4414154 27 4403315 49 4414081 26 4414118 26 4414155 27 4403316 49 4414082 26 4414119 26 4414156 27 4403326 49 4414083 26 4414120 26 4414157 27 4403328 49 4414084 26 4414121 26 4414158 27 4404020 34 4414085 26 4414122 26 4414159 27 4404024 34 4414086 26 4414123 26 4414160 27 4405443 54 4414087 26 4414124 26 4414161 27 4405448 54 4414088 26 4414125 26 4414162 27 4405460 53 4414089 26 4414126 26 4414163 27 4405465 53 4414090 26 4414127 26 4414164 27 4405471 53 4414091 26 4414128 26 4414165 27 4405501 53 4414092 26 4414129 26 4414166 27 4409535 51 4414093 26 4414130 26 4414167 27 4410126 51 4414094 26 4414131 26 4414168 27 4413255 25 4414095 26 4414132 26 4414169 27 4413256 25 4414096 26 4414133 26 4414170 27 4413257 25 4414097 26 4414134 26 4414171 27 4413258 25 4414098 26 4414135 26 4414172 27 4413259 25 4414099 26 4414136 26 4414173 27 4413260 25 4414100 26 4414137 26 4414174 27 4413261 25 4414101 26 4414138 26 4414175 27 4413262 25 4414102 26 4414139 26 4414176 27 4413263 25 4414103 26 4414140 26 4414177 27 4413264 25 4414104 26 4414141 26 4414178 27 4413265 25 4414105 26 4414142 26 4414179 27 4413266 25 4414106 26 4414143 26 4414180 27 4414070 26 4414107 26 4414144 26 4414181 27 4414071 26 4414108 26 4414145 26 4414182 27 4414072 26 4414109 26 4414146 26 4414183 27 4414073 26 4414110 26 4414147 26 4414184 27 4414074 26 4414111 26 4414148 26 4414185 27 4414075 26 4414112 26 4414149 27 4414186 27 4414076 26 4414113 26 4414150 27 4414187 27 65

INDEX 4414188 27 4418735 26 4418772 26 4418809 27 4414189 27 4418736 26 4418773 26 4418810 27 4414190 27 4418737 26 4418774 26 4418811 27 4414191 27 4418738 26 4418775 26 4418812 27 4414192 27 4418739 26 4418776 26 4418813 27 4414193 27 4418740 26 4418777 26 4418814 27 4414194 27 4418741 26 4418778 26 4418815 27 4414195 27 4418742 26 4418779 26 4418816 27 4414196 27 4418743 26 4418780 26 4418817 27 4414197 27 4418744 26 4418781 26 4418818 27 4414198 27 4418745 26 4418782 26 4418819 27 4414199 27 4418746 26 4418783 26 4418820 27 4414200 27 4418747 26 4418784 26 4418821 27 4415440 51 4418748 26 4418785 26 4418822 27 4415450 51 4418749 26 4418786 26 4418823 27 4415452 51 4418750 26 4418787 26 4418824 27 4415461 27 4418751 26 4418788 26 4418825 27 4418715 26 4418752 26 4418789 26 4418826 27 4418716 26 4418753 26 4418790 26 4418827 27 4418717 26 4418754 26 4418791 26 4418828 27 4418718 26 4418755 26 4418792 26 4418829 27 4418719 26 4418756 26 4418793 26 4418830 27 4418720 26 4418757 26 4418794 27 4418831 27 4418721 26 4418758 26 4418795 27 4418832 27 4418722 26 4418759 26 4418796 27 4418833 27 4418723 26 4418760 26 4418797 27 4418834 27 4418724 26 4418761 26 4418798 27 4418835 27 4418725 26 4418762 26 4418799 27 4418836 27 4418726 26 4418763 26 4418800 27 4418837 27 4418727 26 4418764 26 4418801 27 4418838 27 4418728 26 4418765 26 4418802 27 4418839 27 4418729 26 4418766 26 4418803 27 4418840 27 4418730 26 4418767 26 4418804 27 4418841 27 4418731 26 4418768 26 4418805 27 4418842 27 4418732 26 4418769 26 4418806 27 4418843 27 4418733 26 4418770 26 4418807 27 4418844 27 4418734 26 4418771 26 4418808 27 4418845 27 66

INDEX 4418846 27 4441114 24 4448486 24 4461146 55 4425557 51 4441117 24 4448489 24 4461881 22 4425559 51 4441118 24 4448490 24 4461882 22 4425562 51 4442487 49 4448491 24 4461884 22 4425618 51 4442488 49 4448536 54 4462263 55 4426697 27,29 4442520 49 4448549 53 4464058 43 4426698 27,29 4444281 39 4448571 53 4464059 43 4426699 27,29 4444292 39 4448591 53 4464060 43 4426700 27,29 4444303 39 4448592 53 4464061 43 4426701 27,29 4444308 39 4448616 53 4464062 43 4426961 42 4444432 21 4453745 53 4464063 43 4426962 42 4444434 21 4457287 35 4464064 43 4426963 42 4444436 21 4457288 35 4464065 43 4427012 42 4444556 18 4457289 35 4464066 43 4427013 42 4444557 18 4457291 35 4464067 43 4427014 42 4444558 18 4457292 35 4464068 43 4427037 34 4444745 39 4457296 35 4464069 43 4427038 34 4444746 39 4457297 35 4464070 43 4427975 40 4444747 39 4457298 35 4464071 43 4440038 19 4444748 39 4457301 35 4464074 43 4440039 19 4444749 39 4457302 35 4464075 43 4440040 19 4444750 39 4457303 35 4464076 43 4440041 19 4444752 39 4457304 35 4464077 43 4440042 19 4444766 39 4457307 35 4464078 43 4440043 19 4444899 41 4457308 35 4464079 43 4440044 19 4444909 41 4457309 35 4464080 43 4440045 19 4444910 28,41 4457310 35 4464081 43 4440046 19 4444913 28,41 4458235 14 4464084 43 4440047 19 4444963 18 4458236 14 4464085 43 4440048 19 4444964 18 4458237 14 4464086 43 4440049 19 4444965 18 4458366 49 4464087 43 4440878 35 4445552 14 4458367 49 4464088 43 4440879 35 4445553 14 4458368 49 4464089 43 4440880 35 4445554 14 4458369 49 4464092 43 4440886 40 4445555 14 4459405 35 4464093 43 4440887 40 4448484 24 4459407 35 4465804 48 4440888 40 4448485 24 4459408 35 4465805 48 67

INDEX 4465807 48 4485714 22 AM1728 13 N8080119 17 4466037 55 4485715 22 AM1729 13 N8080127 17 4466038 55 4486295 22 AM1812 11 N8080128 17 4467538 48 450003 60 AM1830 11 N8080234 17 4473087 38 450024 60 AM1836 11 N8080260 17 4473088 38 450025 60 AM1837 11 N8080270 17 4482777 22,60 -A- AM1840 11 -Q- 4482778 22,60 A25316 9 AM1907 11 QS3D-PF 9 4482779 22,60 A26358 9 AM1911 11 4483273 57 A26359 9 AM1912 11 4483285 57 A25599 13 AM1914 11 4483315 57 A25600 13 AM1920 11 4483316 57 A25601 13 AM1931 11 4483317 57 A25602 13 AM1939 11 4483320 57 A25603 13 AM7000 11 4483321 57 A25605 13 AM7001 11 4483322 57 AM10026 11 AM7020 11 4483343 57 AM10027 11 AM7021 11 4483349 57 AM10050 11 AM7022 11 4483350 57 AM1540 43 AM7023 11 4483352 57 AM1556 38 AM7024 11 4483354 57 AM1560 38 AM7030 11 4483355 57 AM1561 38 AM9860 11 4483356 57 AM17000 43 AM9861 11 4483395 57 AM17001 43 AM9912 11 4483485 57 AM17003 43 AM9930 20 4483486 57 AM17010 43 AM9932 20 4483487 57 AM17011 43 AM9937 20 4483492 57 AM17012 43 AM9938 20 4483493 57 AM17100 43 AM9939 20 4483494 57 AM17101 43 -I- 4483495 57 AM17103 43 IVF3005 36 4483496 57 AM17110 43 IVF3001 36 4484262 22 AM17111 43 -N- 4484263 22 AM17120 43 N8010560 57 4485712 22 AM17121 43 N8010933 58 4485713 22 AM17150 43 N8080096 17 68

INDEX Customer Defined Anti-miR mirna Inhibitor 43 Customer Defined Pre-miR mirna Precursor 43 Customer-Defined mirvana mirna Inhibitor 43 Customer-Defined mirvana mirna Mimic 43 Cy3 Anti-miR Negative Control 43 Cy3 Pre-miR Negative Control 43 -F- FAM Anti-miR Negative Control 43 FAM Pre-miR Negative Control 43 Fast SYBR Green Cells-to-CT Kit 13 Fast SYBR Green Master Mix 23 -G- GeneAmp dntp Blend 17 -H- High Capacity cdna Reverse Transcription Kit 17 High Capacity RNA-to-cDNA Kit 17 -I- Invivofectamine 3.0 Reagent 36 -L- LAMP for Applied Biosystems 7300/7500/ViiA 7 System 59 LAMP for GeneAmp 5700 and ABI PRISM 7000 System 59 Lipofectamine RNAiMAX 36 -M- MagMAX Total Nucleic Acid Isolation Kit 11 MagMAX Viral RNA Isolation Kit 11 MagMAX -96 Blood RNA Isolation Kit 11 MagMAX -96 Total RNA Isolation Kit 11 MagMAX -96 Viral RNA Isolation Kit 11 Magnetic Stand-96 11 Megaplex PreAmp Primers, Human Pool 39 Megaplex PreAmp Primers, Human Pool Set 39 Megaplex PreAmp Primers, Rodent Pool 39 Megaplex PreAmp Primers, Rodent Pool Set 39 Megaplex Primer Pools, Human Pools 39 Megaplex Primer Pools, Human Pools Set 39 Megaplex Primer Pools, Rodent Pools 39 Megaplex Primer Pools, Rodent Pools Set 39-0- 0.1 mm EDTA 11-2- 20% Glycerol Solution Molecular Biology Grade 17-9- 96 well Magnetic-Ring Stand 11 -A- Ambion In Vivo Custom Designed 35 Ambion In Vivo Custom sirna 35 Ambion In Vivo Factor VII Positive Control sirna, HPLC In Vivo Ready 35 Ambion In Vivo Factor VII sirna, HPLC In Vivo Ready 35 Ambion In Vivo GAPDH Positive Control sirna 35 Ambion In Vivo Negative Control #1 sirna 35 Ambion In Vivo Pre-designed sirna 35 Ambion Single Cell Lysis Kit 14 Ambion Single Cell-to-CT Kit 14 AmpErase Uracil N-glycosylase UNG 17 Anti-miR mirna Inhibitor 43 Anti-miR Negative Control #1 43 -C- Cells-to-CpG Bisulfite Conversion & Quantitation Control Kit 14 Cells-to-CpG Bisulfite Conversion Kit 14 Cells-to-CpG Methylated and Unmethylated gdna Control Kit 14 Cells-to-CT 1-Step TaqMan Kit 13 Cells-to-CT 1-Step Power SYBR Green Kit 13 Cells-to-CT Stop Solution 13 Custom Plus TaqMan Copy Number Assays 49 Custom Plus TaqMan RNA Assay 24 Custom Select sirna 34 Custom TaqMan Copy Number Assays 49 Custom TaqMan Gene Expression Assays 24 Custom TaqMan Small RNA Assays 40 Custom TaqMan SNP Genotyping Assays 45 Custom TaqMan SNP Genotyping Assays for Rare Mutation Analysis 9 69

INDEX Megaplex RT Primers, Human Pool 39 Megaplex RT Primers, Human Pool Set 39 Megaplex RT Primers, Rodent Pool 39 Megaplex RT Primers, Rodent Pool Set 39 MeltDoctor HRM 51 MeltDoctor HRM 51 MeltDoctor HRM 51 MeltDoctor HRM 51 MeltDoctor HRM 51 MicroAmp 48-Well Base Adaptor 59 MicroAmp 96-Well Support Base 59 MicroAmp 96-Well Tray for VeriFlex Blocks 59 MicroAmp 96-Well Tray/Retainer Set 59 MicroAmp 96-Well Tray/Retainer Set for Veriti System 59 MicroAmp Adhesive Film Applicator 58 MicroAmp EnduraPlate Optical 96-Well Fast Plate 57 MicroAmp EnduraPlate Optical 96-Well Plate 57 MicroAmp EnduraPlate Optical 384-Well Plate 57 MicroAmp Fast 48-Well Trays 59 MicroAmp Fast 8-Tube Strip 0.1mL 59 MicroAmp Fast 96-Well Reaction Plate 0.1mL 57 MicroAmp Fast Optical 48-Well Reaction Plate 59 MicroAmp Fast Optical 96-Well Reaction Plate with Barcode 0.1mL 57 MicroAmp Fast Reaction Tube with Cap 0.1mL 59 MicroAmp Optical 384-Well Reaction Plate 57 MicroAmp Optical 384-Well Reaction Plate with Barcode 57 MicroAmp Optical 48-Well Adhesive Film 59 MicroAmp Optical 8-Cap Strip Flat 58 MicroAmp Optical 8-Tube Strip 0.2mL 58 MicroAmp Optical 96-Well Reaction Plate 57 MicroAmp Optical 96-Well Reaction Plate with Barcode 57 MicroAmp Optical 96-Well Reaction Plate with Barcode and Optical Caps 57 MicroAmp Optical Adhesive Film 58 MicroAmp Optical Adhesive Film Kit 58 MicroAmp Optical Film Compression Pad 59 MicroAmp Optical Tube without Cap 0.2mL 58 MicroAmp Splash Free 96-Well Base 59 mirvana mirna inhibitor 43 mirvana mirna inhibitor 43 mirvana mirna Inhibitor let-7 Positive Control 43 mirvana mirna Inhibitor Negative Control #1 43 mirvana mirna Isolation Kit 38 mirvana mirna mimic 43 mirvana mirna mimic mir-1 Positive Control 43 mirvana mirna mimic Negative Control #1 43 mirvana mirna mimics 43 mirvana PARIS Kit 38 MultiScribe Reverse Transcriptase 17 -N- Nuclease-free Water 20 -O- Oligo d(t) 16 Primers 17 -P- Power SYBR Green Cells-to-CT Kit 13 Power SYBR Green PCR Master Mix 23 Power SYBR Green RNA-to-CT 1-Step Kit 15 Pre-miR hsa-mir-1 mirna Precursor 43 Pre-miR mirna Precursor Starter Kit 43 Pre-miR mirna Precursors Molecule 43 Pre-miR Negative Control 43 Protein Expression Lysate Control Kit 54 Protein Expression Sample Prep Kit 54 Protein Quant Sample Lysis Kit 54 Protein Thermal Shift Dye Kit 55 Protein Thermal Shift Starter Kit 55 Protein Thermal Shift 1.0 55 Protein Thermal Shift 55 PureLink DNase Set 10 PureLink Homogenizer 10 PureLink RNA Micro Kit 10 PureLink RNA Mini Kit 10 70

INDEX Single Place Magnetic Stand 11 SuperScript VILO cdna Synthesis Kit 16 SuperScript VILO Master Mix 16 SYBR Green Cells-to-CT Control Kit 13 SYBR Green PCR Core Reagents 23 SYBR Green PCR Master Mix 23 -T- TaqMan Array Gene Signature 96-Well Plate 26,27 TaqMan Array Human Endogenous Control Plate 29 TaqMan Array Human Endogenous Controls 29 TaqMan Array Human MicroRNA Cards 28,41 TaqMan Array Human MicroRNA Cards Set 28,41 TaqMan Array Mouse Endogenous Controls 29 TaqMan Array Plates 25 TaqMan Array Rat Endogenous Controls 29 TaqMan Array Rodent MicroRNA Cards 28,41 TaqMan Array Rodent MicroRNA Cards Set 28,41 TaqMan Custom Arrays 28 TaqMan Cells-to-CT Control Kit 13 TaqMan Control Genomic DNA Human 17 TaqMan Control Total RNA Human 17 TaqMan Copy Number Assays 49 TaqMan Copy Number Reference Assay 49 TaqMan Drug Metabolism Genotyping Assay 45 TaqMan EGFR Exon 19 Deletions Assay 48 TaqMan Endogenous Control 30,31 TaqMan Fast Advanced Master Mix 18 TaqMan Fast Cells-to-CT Kit 13 TaqMan Fast Universal PCR Master Mix 18 TaqMan Fast Virus 1-Step Master Mix 21 TaqMan GAPDH Assay, JUN dye/qsy probe 22 TaqMan GAPDH Assay, JUN dye/qsy probe, primer-limited 22 TaqMan Gene Expression Assays, Inventoried 24,30 TaqMan Gene Expression Assays, Made-to-Order 24 TaqMan Gene Expression Cells-to-CT Kit 13 -Q- QuantStudio 3D PCR 9 QuantStudio 3D PCR Chips 9 QuantStudio 3D PCR Master Mix 9 -R- Random Hexamers 17 RNA later -ICE 11 RNA later Solution 11 RNAqueous Kit 11 RNAqueous Midi Kit 11 RNAqueous -4PCR Kit 11 RNAqueous -96 Automated Kit 11 RNAqueous -96 Kit 11 RNAqueous -Micro Kit 11 RNase Inhibitor 17 -S- Silencer Select GAPDH sirna (Hs, Mm, Rn) 34 Silencer Select Human Druggable Genome sirna Library V4 34 Silencer Select Human Druggable Genome V4 sirna Ext Set 34 Silencer Select Human Extended Druggable Genome sirna Library V4 34 Silencer Select Human Genome sirna Library V4 34 Silencer Select Human Genome sirna V4 Extension Set 34 Silencer Select Human GPCR sirna Library V4 34 Silencer Select Human Ion Channel sirna Library V4 34 Silencer Select Human Kinase sirna Library V4 34 Silencer Select Human Nuclear Hormone Receptor sirna Library V4 34 Silencer Select Human Phosphatase sirna Library V4 34 Silencer Select Human Protease sirna Library V4 34 Silencer Select Negative Control sirna 34 Silencer Select Pre-designed sirna 34 Silencer Select Validated sirna 34 71

INDEX TaqMan Gene Expression Master Mix 20 TaqMan Gene Signature Arrays 28 TaqMan Genotyping Master Mix 46 TaqMan GTXpress Master Mix 46 TaqMan Human Endogenous Control Array 29 TaqMan MGB Probe 60 TaqMan MGB SNP 60 TaqMan MGB 60 TaqMan MicroRNA Assays, Inventoried 40 TaqMan MicroRNA Assays, Made-to-Order 40 TaqMan MicroRNA Cells-to-CT Kit 13 TaqMan MicroRNA RT Kit 39 TaqMan mirna ABC Purification Kit-Human Panel 38 TaqMan Multiplex Master Mix 22 TaqMan Mutation Detection Assays 48 TaqMan Mutation Detection IPC Reagent Kit 48 TaqMan Mutation Detection Reference Assays 48 TaqMan Non-coding RNA Assays 42 TaqMan PCR Core Reagents Kit 21 TaqMan Pre-Designed SNP Genotyping Assays 45 TaqMan PreAmp Cells-to-CT Kit 13 TaqMan PreAmp Master Mix 21 TaqMan PreAmp Master Mix Kit 21 TaqMan Pri-miRNA Assays 42 TaqMan Probe 60 TaqMan Protein Assay Kit 53 TaqMan Protein Assays Buffer Kit 53 TaqMan Protein Assays Core Reagents Base Kit 53 TaqMan Protein Assays Fast MasterMix 53 TaqMan Protein Assays Oligo Probe Kit 53 TaqMan Protein Assays Open Kit 53 TaqMan Protein Expression Core Reagents Kit with MasterMix 53 TaqMan QSY 22,60 TaqMan Reagents Starter Kit 21 TaqMan Reverse Transcription Reagents 17 TaqMan RNA-to-CT 1-Step Kit 15 TaqMan RNase P Assay, ABY dye/qsy probe 22 TaqMan RNase P Assay, ABY dye/qsy probe, primer-limited 22 TaqMan Sample-to-SNP Kit 47 TaqMan sirna Assays, Made-to-Order 35 TaqMan TAMRA Probe 60 TaqMan Universal Master Mix II, no UNG 19 TaqMan Universal Master Mix II, with UNG 19 TaqMan Universal PCR Master Mix 20 TaqMan Universal PCR Master Mix, No AmpErase UNG 20 TE Buffer ph7.0 11 THE RNA Storage Solution 11 TRIzol LS Reagent 10 TRIzol Plus RNA Purification System 10 TRIzol Reagent 10 TURBO DNA-free Kit 11 72

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