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1 皮膚バリア保護的に働く弱酸性ポリエステルを利用した化繊アレルギー原因物質の解明 Investigation of Causes of Clothing Dermatitis - Absorption Test using Weak- Acidic Polyethylene Terephthalate and PET by Ryoji Hirota Kochi Medical School Momoe Ukaji Otsuma Women s University ABSTRACT Polyethylene terephthalate PET fabrics are frequently used in human clothes but are irritant in direct contact with the skin. Although sportswear made of weakacidic PET fabrics did not induced skin eczema on irritant contact dermatitis ICD patient, this mechanism was not clear. We performed the patch test using both fabrics in medical students from Kochi University in order to determine whether the skin condition was related to clothing. And we also demonstrated the metal absorption test of synthetic sweat using an inductively coupled plasma mass spectrometry ICP-MS In addition we demonstrated a protein analysis of sweat from participant using a SDS polyacrylamide gel electrophoresis PAGE Thirteen of participants have developed contact dermatitis at the time of this study. There was positive corelation between positive participants of patch test and participants who had past history of clothing dermatitis. We applied synthetic sweat on each fabric and metals were measured. Pb, Cu, Al were strongly absorbed by both fabric, but remaining metals were not Vol.

2 absorbed. After absorption to fabrics, proteins in sweat were separated by SDS-PAGE, but there were no difference in the gel at this time. 要旨 ICP-MS SDS-PAGE. p=. ICP-MS Pb Cu Al 緒言 QOL,. %. %. %. %. % Vol.

3 . 方法. 対象..2 アンケート調査内容.3 パッチテスト WAP REG.4 汗の採取..5 人工汗の調整 Al B Cd Cr Cu Fe Mo Mn Na Ni Pb Zn ppm mol/ HNO Q ppm ph.. ppm ph..6 ポリエステル繊維への元素吸収試験 WAP REG. cm No textile Q.. Q No textile.. ppm Terbium.7 ICP-MS 分析 ICP-MS x No textile SD.8 SDS ポリアクリルアミド (SDS-PAGE) 解析.. Q Vol.

4 . Loading. - SDS e -ATTO II.9 統計解析 SPSS ver. Fisher's exact test ICP-MS multiple analysis of variance MANOVA p. 2. 結果図表 p=. Fisher's exact test 2. 衣服による皮膚障害の経験の有無. %. %. %. % 図 REG と WAP のパッチテスト REG WAP p. 表パッチテスト陽性率. REG. WAP.. 図 2.3 既往歴とパッチテストの関連表. Fisher's extact test p=. 表 2 WAP REG REG REG WAP WAP p=. 2.5 ポリエステル繊維への元素吸収条件 REG WAP. ppm ppm ICP-MS. ppm 図 2 Al Cr Cu Pb Vol.

.2 Cr.4 Cu.2 ppm WAP ppm REG.ppm WAP.ppm REG ppm WAP ppm REG.ppm WAP.ppm REG NO-textile 3.2 Al.2 NO-textile 3 Pb ppm WAP ppm REG.ppm WAP.ppm REG ppm WAP ppm REG.ppm WAP.ppm REGP NO-textile 3 図 2 WAP REG *, X,+,# ;p <.

5 .2 Cr.4 Cu.2 ppm WAP ppm REG.ppm WAP.ppm REG ppm WAP ppm REG.ppm WAP.ppm REG NO-textile 3.2 Al.2 NO-textile 3 Pb ppm WAP ppm REG.ppm WAP.ppm REG ppm WAP ppm REG.ppm WAP.ppm REGP NO-textile 3 図 2 WAP REG *, X,+,# ;p <., **,xx,++,## ; p<. vs. NO-textile NO-textile ポリエステル繊維の元素吸収率 REG WAP Al B Cd Cr Cu Fe Mo Mn Na Ni Pb Zn. ppm 表 3Pb Cr Cu Na Mn 表 3 WAP REG Na - % % Al % % Cr % % Mn - % - % Fe - % % Ni - % - % Cu % % Zn % % Cd % % Pb % % % % Fe 2.7 被験者の汗の蛋白質分析 Sample Sample Sample WAP REG SDS-PAGE KDa Sample Sample WAP REG 図 3 3. 考察 Vol.

(kda) Sample Sample2 Sample3 25 75 5 25 5-2% 図 3 p=. Mizutani et al vs.

6 (kda) Sample Sample2 Sample % 図 3 p=. Mizutani et al vs. Pb Cu Al Cr Ni Cr Ni WEP REG Cr REG Dai et al. - - NF- B SDS- PAGE WAP REG 4. 結論 Vol.

7 ICP-MS Pb Cu Al 謝辞文献 34: - G.Ricci, A.Patrizi, F.Bellini, M.Medri, Use of Textiles in Atopic Dermatitis: Care of Atopic Dermatitis., Curr. Probl. Dermatol., 33: - E.Dawes-Higgs, S.Freeman, Allergic Contact Dermatitis Caused by the Clothing Dye, Disperse Blue, an Important Contact Allergen That May Be Frequently Missed., Australas J. Dermatol., 45: -, 9 : - R.Hirota, C.Mizutani, Y.Kawanoue, Prevalence of Clothing-Caused Contact Dermatitis among Japanese Female Adults., KMS / / - C.Mizutani, M.Ukaji, N.Horikawa, T.Yamamoto, K.Kajiwara, Study of Weak-Acidic Clothing Materials Developed for Skin Conditioning., SEN'I GAKKAISHI, 69, : - X.Dai, H.Okazaki, Y.Hanakawa, M.Murakami, M.Tohyama, Y.Shirakata, K.Sayama, Eccrine Sweat Contains Il- alpha, Il- beta and Il- and Activates Epidermal Keratinocytes as a Danger Signal., PLoS One, 8: e Vol.

220 28;29) 30 35) 26;27) % 8.0% 9 36) 8) 14) 37) O O 13 2 E S % % 2 6 1fl 2fl 3fl 3 4

220 28;29) 30 35) 26;27) % 8.0% 9 36) 8) 14) 37) O O 13 2 E S % % 2 6 1fl 2fl 3fl 3 4 Vol. 12 No. 2 2002 219 239 Λ1 Λ1 729 1 2 29 4 3 4 5 1) 2) 3) 4 6) 7 27) Λ1 701-0193 288 219 220 28;29) 30 35) 26;27) 0 6 7 12 13 18 59.9% 8.0% 9 36) 8) 14) 37) 1 1 1 13 6 7 O O 13 2 E S 1 1 17 0 6 1 585