untitled
|
|
|
- さあしゃ なかきむら
- 5 years ago
- Views:
Transcription
1 Mx3000P & Mx3005P Real-Time QPCR System MxPro Software version 4.10 Revision#
2 Mx3000P & Mx3005P Real-Time QPCR System 1
3 PCR Stratagene SYBR Green I PCR Brilliant II FAST SYBR QPCR Master Mix Cat# Brilliant II SYBR Green QPCR Master Mix Cat# Brilliant II SYBR Green QPCR Low ROX Master Mix Cat# Brilliant II SYBR Green QRT-PCR Master Mix, 1-step Cat# Brilliant II SYBR Green QRT-PCR Low ROX Master Mix, 1-step Cat# Brilliant II SYBR Green QRT-PCR AffinityScript Master Mix, 2-step Cat# Brilliant SYBR Green Core Reagent Kit Cat# PCR Brilliant II FAST QPCR Master Mix Cat# Brilliant II QPCR Master Mix Cat# Brilliant II QPCR low ROX Master Mix Cat# Brilliant II QRT-PCR Master Mix, 1-step Cat# Brilliant II QRT-PCR low ROX Master Mix, 1-step Cat# Brilliant II QRT-PCR AffinityScript Master Mix, 2-step Cat# Brilliant II QRT-PCR Core Reagent Kit, 1-step Cat# Brilliant Multiplex QPCR Master Mix Cat# SideStep SideStep SYBR Green QPCR Master Mix Cat# SideStep II SYBR Green QRT-PCR Master Mix, 2 Step Cat# SideStep II QRT-PCR Master Mix, 1-step Cat# SideStep II QRT-PCR Master Mix, 2-step Cat# SideStep Lysis and Stabilization Buffer Cat# ml SideStep Cell Lysate Analysis Kit Cat# SideStep II QPCR cdna Synthesis Kit Cat# cdna & AffinityScript QPCR cdna Synthesis Kit Cat# AffinityScript Multiple Temperature Reverse Transcriptase Cat# Mx3000P & Mx3005P Real-Time QPCR System 2
4 RNA Stratagene QPCR Reference Total RNA, Human Cat# µg Stratagene QPCR Reference Total RNA, Mouse Cat# µg Universal Human mirna Reference RNA Cat# µg mirna High-Specificity mirna QRT-PCR Detection Kit Cat# mirna QPCR Master Mix Cat# mirna QRT-PCR First strand cdna Synthesis Kit Cat# High-Specificity mirna QPCR Core Kit Cat# QPCR Alien QRT-PCR Inhibitor Alert Cat# Alien Reference RNA QRT-PCR Detection Kit (FAM-detection) Cat# Alien Reference RNA QRT-PCR Detection Kit (VIC-detection) Cat# RNA Absolutely RNA Miniprep Kit Cat# Absolutely RNA Microprep Kit Cat# Absolutely RNA Nanoprep Kit Cat# Absolutely RNA 96 Microprep Kit Cat# plates Absolutely mrna Purification Kit Cat# Absolutely RNA FFPE Kit Cat# Absolutely RNA mirna Kit Cat# Mx3000P Mx3005P Mx3000P / Mx3005P Replacement Bulb Assembly Cat# bulb 96-Well QPCR Plate, Semi-Skirted Cat# plates 96-Well QPCR Plate, Non-Skirted Cat# plates Mx3000P / Mx3005P Strip tubes Cat# strips Mx3000P / Mx3005P Optical Strip Caps Cat# strips Single PCR Reaction Tubes (Without Caps) Cat# bag MxP Perfect Frame Cat# frame Mx3000P & Mx3005P Real-Time QPCR System 3
5 Brilliant master mix Brilliant 2.5 ml Reference dye (ROX 1mM) 0.5µl ROX Reference dye DW 200 ROX 20µl 0.3µl ROX 20µl SYBR Green 2x Master Mix 10µL 10µL Primer xµl xµl Probe none xµl Template xµl xµl DW Up to 20µL Up to 20µL Mx3000P/Mx3005P PCR 10µl 60µl standard curve QPCR Mx3000P/Mx3005P Mx3000P & Mx3005P Real-Time QPCR System 4
6 Mx3000P/Mx3005P / Mx3000P/Mx3005P ON Mx3000P/Mx3005P PC ON Mx3000P/Mx3005P 96 well 8 Optical cap Mx3000P & Mx3005P Real-Time QPCR System 5
7 8 96 well / Mx3000P/Mx3005P Mx3000P / Mx3000P & Mx3005P Real-Time QPCR System 6
8 MxPro Ver.4.10 MxPro Mx Pro Multiple Experiment Analysis Mx3000P/ Mx3005P PC PC Quantitative PCR (Multiple Standards) TaqMan probe Molecular Beacon Standard curve Mx3000P Mx3005P QPCR Dissociation curve Comparative Quantification (Calibrator) Ct Standard TaqMan probe SYBR Green Dissociation curve Standard curve QPCR SYBR Green (with Dissociation Curve) SYBR Green Dissociation curve Mx3000P & Mx3005P Real-Time QPCR System 7
9 EvaGreen (with Dissociation Curve) SYBR Green EvaGreen Stratagene High Specificity mirna Detection kit Allele Discrimination / SNP s Real-Time Molecular Beacon amplification plot SNP Molecular Beacon Melting Curve Molecular Beacon Quantitative Plate Read Standard curve SYBR Green PicoGreen RiboGreen EvaGreen Plate Read / Allele Discrimination TaqMan probe SNP Multiple Experiment Analysis Standard curve Mx3000P & Mx3005P Real-Time QPCR System 8
10 Mx Pro Setup Run Analysis Multiple Experiment Analysis Project Setup Setup Plate Setup Thermal Profile Thermal Profile Run Run Thermal Profile Status Raw data plots Mx3000P/Mx3005P Analysis Analysis Analysis Selection / Setup Results Project Project Project Setup Project Results Mx3000P & Mx3005P Real-Time QPCR System 9
11 Plate Setup Experimental type Quantitative PCR (Multiple Standards) SYBR Green (with Dissociation curve) Import mxp Defaults Quick Setup Mx3000P & Mx3005P Real-Time QPCR System 10
12 Well type Well type Show Well Names Well Information Well Name Collect fluorescence data Mx3000P/Mx3005P MxPro Mx3000P/Mx3005P Reference dye Reference dye Assign Assay Names Well Information Well name Comments Assign Standard Quantity Standard template Auto Increment Standard Units Standard template Identify replicates Replicate symbol replicate well Threshold line Minimum Ct spread algorithm replicate symbol Clear Selected Wells Mx3000P & Mx3005P Real-Time QPCR System 11
13 Comparative quantification (Calibrator) Well type Comparative quantification Calibrator Normalizing assay Ct Association symbol SYBR Green Normalizer Unknown Normalizer Association symbol Mx3000P & Mx3005P Real-Time QPCR System 12
14 Undo Redo On / Off MxPro ver.4.1 ON 20 PC MxPro Replicate Wells treatment setting Analysis Term Setting Threshold line Baseline Amplification plot Chart Mx3005P Mx3000P Mx3000P & Mx3005P Real-Time QPCR System 13
15 Plate Setup Quick Protocol Mx3000P & Mx3005P Real-Time QPCR System 14
16 Plate Setup Quick Protocol (SYBR Green ) DNA SYBR Green I PCR SYBR Green PCR PCR standard template NTC Sample # Gene Well type Filter Conc. Well 1 A unknown SYBR A1-A3 2 A unknown SYBR B1-B3 3 A unknown SYBR C1-C3 4 B unknown SYBR A4-A6 5 B unknown SYBR B4-B6 6 B unknown SYBR C4-C6 7 A standard SYBR 10 6 A7-A9 8 A standard SYBR 10 5 B7-B9 9 A standard SYBR 10 4 C7-C9 10 A standard SYBR 10 3 D7-D9 11 A NTC SYBR E7-E9 12 B standard SYBR 10 6 A10-A12 13 B standard SYBR 10 5 B10-B12 14 B standard SYBR 10 4 C10-C12 15 B standard SYBR 10 3 D10-D12 16 B NTC SYBR E10-E12 Mx3000P & Mx3005P Real-Time QPCR System 15
17 MxPro SYBR Green SYBR Green (with Dissociation Curve a) # Well type unknown Mx3000P & Mx3005P Real-Time QPCR System 16
18 b) unknown SYBR Collect fluorescence data SYBR FAM ROX ROX Reference dye ROX Filter SYBR Collect fluorescence data FAM SYBR FAM Mx3000P/Mx3005P PC PC Mx3000P/Mx3005P PC Mx3000P & Mx3005P Real-Time QPCR System 17
19 SYBR Green FAM SYBR SYBR SYBR Green FAM FAM SYBR Green SYBR ROX REF c) Well A4 C6 d) #7 #11 standard NTC Mx3000P & Mx3005P Real-Time QPCR System 18
20 standard #7 #10 well A7 D9 e) Well type Standard Standard f) SYBR ROX ROX Reference dye Mx3000P & Mx3005P Real-Time QPCR System 19
21 g) #11 NTC well E7 E9 Well type NTC SYBR ROX ROX Reference dye h) (e) (g) Mx3000P & Mx3005P Real-Time QPCR System 20
22 i) Replicate symbol well A1 A3 well B1 B3 Replicate symbol Replicate #1 well A1 A3 Replicate Symbol Auto-increment Replicate symbol Mx3000P & Mx3005P Real-Time QPCR System 21
23 j) copies Standard Units Copies #7 # copies Mx3000P & Mx3005P Real-Time QPCR System 22
24 A7 A12 Auto-Increment -10x Auto-Increment Standard quantity SYBR SYBR all Auto-Increment Auto- Increment 10 6 copies 1/10-10 #8 # copies Auto-Increment Auto-Increment Mx3000P & Mx3005P Real-Time QPCR System 23
25 k) triplicate standard Replicate #1 #3 #7 #11 A Well Information SYBR Assay Assay Color Assay A Assay Color Mx3000P & Mx3005P Real-Time QPCR System 24
26 #4 #6 #12 #16 B Mx3000P & Mx3005P Real-Time QPCR System 25
27 Plate Setup Quick Protocol (Probe ) TaqMan probe Molecular Beacon PCR MxPro Quantitative PCR (Multiplex Standards) TaqMan probe Molecular beacon Simple assay TaqMan probe PCR FAM TaqMan probe A VIC TaqMan probe B standard template NTC Mx3000P & Mx3005P Real-Time QPCR System 26
28 Sample # Gene Well type Fileter Conc. Well 1 A unknown FAM A1-A3 2 A unknown FAM B1-B3 3 A unknown FAM C1-C3 4 B unknown HEX A4-A6 5 B unknown HEX B4-B6 6 B unknown HEX C4-C6 7 A standard FAM 10 6 A7-A9 8 A standard FAM 10 5 B7-B9 9 A standard FAM 10 4 C7-C9 10 A standard FAM 10 3 D7-D9 11 A NTC FAM E7-E9 12 B standard HEX 10 6 A10-A12 13 B standard HEX 10 5 B10-B12 14 B standard HEX 10 4 C10-C12 15 B standard HEX 10 3 D10-D12 16 B NTC HEX E10-E12 a) # A unknown triplicate Well type unknown Mx3000P & Mx3005P Real-Time QPCR System 27
29 b) unknown FAM TaqMan probe Collect fluorescence data FAM ROX ROX Reference dye ROX FAM ROX REF Mx3000P & Mx3005P Real-Time QPCR System 28
30 c) FAM TaqMan probe VIC TaqMan probe FAM HEX VIC HEX Well A4 C6 d) #7 #11 standard NTC standard #7 #10 well A7 D9 Mx3000P & Mx3005P Real-Time QPCR System 29
31 e) Well type Standard Standard f) FAM ROX ROX Reference dye g) #11 NTC well E7 E9 Well type NTC FAM ROX ROX Reference dye Mx3000P & Mx3005P Real-Time QPCR System 30
32 h) (e) (g) FAM HEX i) Replicate symbol well A1 A3 well B1 B3 Replicate symbol Mx3000P & Mx3005P Real-Time QPCR System 31
33 Replicate #1 well A1 A3 Replicate Symbol Auto-increment Replicate symbol j) FAM HEX 12 [all] 12 Mx3000P & Mx3005P Real-Time QPCR System 32
34 Standard quantity Standard units Standard units copies relative milimole Standard quantity FAM HEX Standard quantity all Replicate #7(well A7 A9) Replicate #13 (well A10 A12) standard all Auto-Increment -10x Auto-Increment Standard Mx3000P & Mx3005P Real-Time QPCR System 33
35 FAM A HEX (VIC) B SYBR Green Well information FAM FAM standard standard curve FAM HEX (VIC) Well information FAM HEX (VIC) Well information Well information FAM SYBR Green Eva Green FAM SYBR Green unknown standard Well information Well information SYBR Green Mx3000P & Mx3005P Real-Time QPCR System 34
36 Multiplex assay FAM HEX VIC Multiplex Sample # Gene Well type Filter Conc. Well 1 A/B unknown FAM & HEX A1-A3 2 A/B unknown FMA & HEX B1-B3 3 A/B unknown FAM & HEX C1-C3 4 A/B standard FAM & HEX 10 6 A4-A6 5 A/B standard FAM & HEX 10 5 B4-B6 6 A/B standard FAM & HEX 10 4 C4-C6 7 A/B standard FAM & HEX 10 3 D4-D6 8 A/B NTC FAM & HEX E4-E6 Multiplex a) unknown A1 C3 Well type unknown FAM HEX ROX Reference dye ROX Mx3000P & Mx3005P Real-Time QPCR System 35
37 b) Standard well A4 - D6 Well A4 D6 Well type standard FAM HEX ROX Reference Dye ROX c) NTC Well E4 E6 Well E4 E6 Well type NTC FAM HEX ROX Reference Dye ROX d) Replicate symbol Mx3000P & Mx3005P Real-Time QPCR System 36
38 e) Standard Standard Units Standard Quantities Auto-Increment Simple Multiplex assay Well information Well information Multiplex assay Mx3000P & Mx3005P Real-Time QPCR System 37
39 Plate Setup Quick Protocol ( ) SYBR Green SYBR Green A B (b-actin) A B RSq NTC 20µl 3 Sample # Gene Well type Filter Description Well #1 A calibrator SYBR A1-A3 #2 A unknown SYBR B1-B3 #3 A unknown SYBR C1-C3 #4 B calibrator SYBR A4-A6 #5 B unknown SYBR B4-B6 #6 B unknown SYBR C4-C6 #7 b-act calibrator SYBR A7-A9 #8 b-act unknown SYBR B7-B9 #9 b-act unknown SYBR C7-C9 Mx3000P & Mx3005P Real-Time QPCR System 38
40 Sample # Gene Well type Filter Conc. Well #10 A standard SYBR 1 x D1-D3 #11 A standard SYBR 1/10 x E1-E3 #12 A standard SYBR 1/100 x F1-F3 #13 A standard SYBR 1/1000 x G1-G3 #14 A NTC SYBR H1-H3 #15 B standard SYBR 1 x D4-D6 #16 B standard SYBR 1/10 x E4-E6 #17 B standard SYBR 1/100 x F4-F6 #18 B standard SYBR 1/1000 x G4-G6 #19 B NTC SYBR H4-H6 #20 b-act standard SYBR 1 x D7-D9 #21 b-act standard SYBR 1/10 x E7-E9 #22 b-act standard SYBR 1/100 x F7-F9 #23 b-act standard SYBR 1/1000 x G7-G9 #24 b-act NTC SYBR H7-H9 MxPro Comparative Quantification (Calibrator) Quantitative PCR SYBR Green Comparative Quantification Mx3000P & Mx3005P Real-Time QPCR System 39
41 a) #1 #1 well A1 A3 A Calibrator Well A1 A3 well type Calibrator Comparative quantification Multiplex 50 SYBR ROX Reference dye ROX Well A1 A3 SYBR Green p17 p18 Mx3000P & Mx3005P Real-Time QPCR System 40
42 b) #2 #3 #2 #3 well B1 C3 A Unknown a Well B1 C3 Well type Unknown SYBR ROX Reference dye ROX c) #4 B Calibrator #5 #6 B Unknown Reference dye A d) normalizer #7 #9 a c #7 b-actin Calibrator #8 #9 b-actin Unknown Mx3000P & Mx3005P Real-Time QPCR System 41
43 e) #10 #13 Standard #14 NTC #10 #13 Well D1 G3 Well type Standard SYBR ROX Reference dye ROX Well H1 H3 #14 NTC Well H1 H3 Well type NTC (No Template Control) SYBR ROX ROX Reference dye Mx3000P & Mx3005P Real-Time QPCR System 42
44 f) #15 #19 B Standard NTC #20 #24 b-actin Standard NTC e #15 #19 Well D4 H6 #20 #24 Well D7 H9 g) Replicate symbol Association symbol Mx3000P & Mx3005P Real-Time QPCR System 43
45 h) #1 #3 #10 #14 A Well information A #1 #3 #10 #14 Well A1 Well H3 Well information SYBR SYBR Assay A Assay Color Mx3000P & Mx3005P Real-Time QPCR System 44
46 A #4 #6 #15 #19 B #7 #9 #20 #24 b-actin Well information Mx3000P & Mx3005P Real-Time QPCR System 45
47 i) Standard #10 #15 #20 Well D1 Well D9 Standard quantity 1 #11 #15 #21 1/10 #12 #17 #22 1/100 #13 #18 #23 1/1000 Auto Increment Standard Mx3000P & Mx3005P Real-Time QPCR System 46
48 j) b-actin Normalizer Normalizer Well type b-actin #7 #9 Calibrator #7 #7 Well A7 A9 Normalizing assay b-act Well A7 A9 b-act NORM Unknown #8 #9 Normalizer Normalizer Well type Calibrator Unknown Mx3000P & Mx3005P Real-Time QPCR System 47
49 normalizer MxPro version 4.10 Normalizer BRCA Replicate #1 4 GAPDH Replicate #5 8 18S Replicate #9 12 GUS Replicate #13 16 Normalizer Normalizing assay GAPDH 18S GUS Normalizer Ct Ct Normalizer Mx3000P & Mx3005P Real-Time QPCR System 48
50 k) Association #1 A #4 B #7 b-act Well A1 A9 Well A1 A9 Assoc. Symbol A A Well B1 B9 Well C1 C9 B C Mx3000P & Mx3005P Real-Time QPCR System 49
51 Standard Association symbol Standard Standard curve Relative quantity to Standard Multiplex Cy5 b-actin FAM A VIC B normalizer b-actin association A FAM B VIC Multiplex b-actin SYBR Green Association Mx3000P & Mx3005P Real-Time QPCR System 50
52 normalizer Multiplex normalizer LIMS MxPro version 4.10 LIMS (Laboratory Information Management System) LIMS LIMS ASCII Mx3000P & Mx3005P Real-Time QPCR System 51
53 Thermal Profile Setup Quick Protocol Quantitative PCR (Multiple Standards) Comparative Quantification (Calibrator) Thermal Profile SYBR Green Eva Green Dissociation curve SYBR Green Comparative quantification Dissociation curve Dissociation curve profile Quantitative PCR Comparative Quantification Experimental type Thermal Profile Thermal Profile Thermal profile Thermal Profile Design Standard Pre-Melt / RT Segment(s) Amplification Segment Dissociation / Melt Segment Mx3000P & Mx3005P Real-Time QPCR System 52
54 Thermal Profile Design Custom Add Segment Plateau with Ramp Data collection marker by dragging All point End point Thermal Profile Plateau Ramp Ramp Ramp 0.2 scan Ramp Dissociation curve Plateau Ramp Plateau Selection Plateau Ramp Segment Property Import Thermal Profile Import Custom Defaults Thermal Profile Mx3000P & Mx3005P Real-Time QPCR System 53
55 Thermal Profile Plateau Plateau Ramp Segment Segment Plateau Thermal Profile Data collection marker Data collection marker Thermal Profile Data collection marker Plateau Ramp Ramp All point Data collection marker Ramp 0.2 scan Mx3000P & Mx3005P Real-Time QPCR System 54
56 Running the Experiment Quick Protocol PCR Lamp Ready Lamp Start-up Run RUN Run Status Stopped Mx3000P/Mx3005P Turn lamp off at end of run Start Run Run Status Stopped Mx3000P/Mx3005P Warming Up 20 Run After Warm-Up 20 Run Now 20 Thermal Profile Mx3000P & Mx3005P Real-Time QPCR System 55
57 Raw Data Plots Raw Data Plots Raw Data Plots Show Plots well Analysis X Y Raw Data Plots Mx3000P & Mx3005P Real-Time QPCR System 56
58 Mx3000P/Mx3005P Run Status Running Add Cycle Thermal Profile PCR Run Status Add cycle Pause Run Pause Thermal profile Run Run Continue Continue experiment at end of run Run Mx3000P/ Mx3005P PC Mx3000P & Mx3005P Real-Time QPCR System 57
59 Data Analysis Quick Protocol Analysis / Selection Setup Quantitative PCR (Multiple standard) Analysis Analysis Selection / Setup Select data Collection ramp / plateau Exclude Assays Mx3000P & Mx3005P Real-Time QPCR System 58
60 Replicates Treat individually Treat collectively Algorithm enhancements Amplification-based threshold Adaptive baseline Moving average Analysis Term Setting Analysis Term Setting Adv. Algorithm Settings Data Collection Marker Average SYBR Green Select data collection ramp/plateau: Dissociation curve Dissociation Data Dissociation curve Mx3000P & Mx3005P Real-Time QPCR System 59
61 Results Results Analysis / Selection Setup Customized Tool bar Mx3000P & Mx3005P Real-Time QPCR System 60
62 Amplification plots Area to analyze Show Standard Curve Amplification plot Standard curve Last cycle Amplification plot endpoint Fluorescence R (Multicomponet view) dr Rn drn Threshold fluorescence Threshold line Threshold line Threshold line Analysis / Selection Setup Threshold line Select amplification plots to display Mx3000P & Mx3005P Real-Time QPCR System 61
63 Fluorescence R (Multicomponent) dr Rn drn Show Standard Curve Amplification plot Standard curve Standard curve RSq Eff amplification plot threshold line RSq Eff Mx3000P & Mx3005P Real-Time QPCR System 62
64 Plate sample values Ct Standard Curve Show Amplification Plots Standard curve Amplification Plots Show confidence interval: confidence interval Threshold fluorescence Standard curve threshold line Initial template quantity calculator Ct Amplification efficiency calculator Slope Eff Mx3000P & Mx3005P Real-Time QPCR System 63
65 Initial template quantity standard curve Ct Text Report File Power Point Mx3000P & Mx3005P Real-Time QPCR System 64
66 amplification plot amplification plot Options Preferences Preferences Text Report tooltips Mx3000P & Mx3005P Real-Time QPCR System 65
67 amplification plot Ctrl Shift Show Amplification Plots Mx3000P & Mx3005P Real-Time QPCR System 66
68 Consolidated Report Consolidated report Dissociation Curve (for SYBR or Eva Green Assays) Dissociation curve SYBR Green Eva Green Experimental type Results Quantitative PCR Experimental type Thermal profile Dissociation curve Results Dissociation curve Experimental type SYBR Green Experimental type Dissociation curve Dissociation curve Experimental Type SYBR Green Eva Green Results Dissociation curve For ramp dissociation use cycle: Cycle increment Dissociation curve Ramp All point Fluorescence Mx3000P & Mx3005P Real-Time QPCR System 67
69 R (Multicomponent view) Rn -R (T) -Rn (T) Product melting temperature (Tm) Tm Min peak height Dissociation curve Tm Experimental type Experimental type File Convert Experimental Type Mx3000P & Mx3005P Real-Time QPCR System 68
70 Relative quantity (Comparative quantification ) Comparative quantification Experimental type Relative quantity_chart Relative quantity_plate Relative quantity chart Calibrator Ct Show fold change log2 Fold change Show error bar Graph Properties X Y p74 Mx3000P & Mx3005P Real-Time QPCR System 69
71 Relative quantity plate Calibrator Text report Calibrator Standard Rel. Quant. to Std) Normalizer (house keeping gene ) Rel. Quant. to Norm. Mx3000P & Mx3005P Real-Time QPCR System 70
72 Ct MxPro Analysis Term Setting Efficiency Setting 100% Normalizer Target standard curve Standard curve Efficiency Setting Eff Eff = Efficiency Mx3000P & Mx3005P Real-Time QPCR System 71
73 Threshold line Baseline Moving average Analysis Term Setting Threshold line Threshold Fluorescence Baseline Baseline Correction Baseline Moving average Smoothing Moving average Mx3000P & Mx3005P Real-Time QPCR System 72
74 Graph Properties Amplification plot Dissociation curve Standard curve Dual color plot Relative quantity chart Graph Properties X axis Y axis Ledgend On/Off Bkgd colors Style X-Axis Label Plate Setup Well ID Mx3000P & Mx3005P Real-Time QPCR System 73
75 Multiple experiments analysis MxPro Ver.4.10 Mx3000P Mx3005P Thermal profile Exp.1.mxp unknown Standard curve Exp.2.mxp unknown Exp.1.mxp Standard curve Exp.2.mxp Exp.1.mxp Exp.2.mxp amplification plot Ct threshold line Standard curve Mx3000P & Mx3005P Real-Time QPCR System 74
76 MxPro New Options Multiple Experiment Analysis Multiple Experiment Analysis Mx3000P/Mx3005P PC Standalone Multiple Experiment Analisys Add Experiments Finish Remove Experiments Multiple experiment analysis threshold line MxPro ver.4.10 Multiple experiment analysis threshold line Mx3000P & Mx3005P Real-Time QPCR System 75
77 threshold line Method 1) Calculate thresholds for individual experiments method threshold line new project Background-Based Threshold Algorithm Amplification-Based Threshold Algorithm Backgroung-Based Threshold Algofithm threhshold line method Data view type By Experiment threhshold value threhshold line By Assay Consolidated Data view type threshold line threshold line Background based Analysis Term Setting Threshold Fluorescence Background-based threshold Cycle range Background-based threshold Mx3000P & Mx3005P Real-Time QPCR System 76
78 Backgroung-based threshold threshold line amplification plot ROX threshold line Setup amplification plot Threshold fluorescence threshold line Lock threshold line threshold line standard curve amplification plot ROX standard IRC (Inter-run control) Method 3 threshold line Method 2) Use common threshold method threhshold line MxPro Project assay threshold line threshold line Ct Data view type By Experiment, By Assay, Consolidated threshold line Mx3000P & Mx3005P Real-Time QPCR System 77
79 Method 3) Use interrun calibrators Interrun calibrator (IRC) Run Ct threshold line Multiple experiment analysis Hellemans et.al., Genome Biology, 2007; 8(2): R19 IRC Well Id IRC Select IRC Wells IRC Multi Experiment Analysis (1) Well name (2) Assay name Well information Well name Assay name Mx3000P & Mx3005P Real-Time QPCR System 78
80 Multi Experiment Analysis Project Project Setup Setup Run Analysis Areas per page Project Results Project Results Area to analyze Data view taype By Experiment By Assay Consolidated Mx3000P & Mx3005P Real-Time QPCR System 79
81 Amplification plot amplification plot Fluorescence drn dr Setup Plate sample value Ct Mx3000P & Mx3005P Real-Time QPCR System 80
82 Standard curve Consolidated Amplification plot Ct Standard curve Consolidated By Experiment By Assay Initial template quantity Standard curve Text report Columun Mx3000P & Mx3005P Real-Time QPCR System 81
83 MxPro Ver.4.10 Normalizer Multiple Experiment Analysis Normalizer 3 samples Multiple experiment analysis threshold line Comparative quantification Multiple experiment analysis threshold line p77 Mx3000P & Mx3005P Real-Time QPCR System 82
84 house keeping gene A B C Inter-run control (Standard) Normalizer Mx3000P & Mx3005P Real-Time QPCR System 83
85 A B C Normalizer Comparative quantification Experiment type Convert A B C Normalizer Well information Well Name Well name Mx3000P & Mx3005P Real-Time QPCR System 84
86 Replicate #1 Sample 01 Name Sample 01 A B C Name Sample 01 Assay GAPDH sample 02 sample 06 Standard Show Well Names Well Name Mx3000P & Mx3005P Real-Time QPCR System 85
87 GAPDH Normalizer Calibrator Replicate #1 sample 01 Calibrator Association symbol Sample Normalizer Name Mx3000P & Mx3005P Real-Time QPCR System 86
88 Show Well Names Name Assign Assay Name Well information Name Assay Name Association symbol Multi Experiment Analysis Association symbol Invalid Multi Experiment Analysis Multi Experiment Analysis Relative quantity chart Standard NTC Project Standard curve Mx3000P & Mx3005P Real-Time QPCR System 87
89 standard curve normalizer Multiple experiment analysis normalizer Threshold line threshold line Project Cross experiment threshold calculation Use interrun calibrators Interrun Calibrators Mx3000P & Mx3005P Real-Time QPCR System 88
90 Project Project Results Project Amplification plot By assay By experiment Consolidated Assay per page Mx3000P & Mx3005P Real-Time QPCR System 89
91 Standard curve Relative quantity chart Mx3000P & Mx3005P Real-Time QPCR System 90
92 Multiplate Comparative assay Mx3000P & Mx3005P Real-Time QPCR System 91
93 Quantitative Plate Read Analysis Quick Protocol Set Read properties SNP Thermal cycling Set Read Properties Instrument data Plate Read Start Options Pre-Read Post-Read Pre-Read Post-Read Pre Post Run Status - Stopped Mx3000P & Mx3005P Real-Time QPCR System 92
94 Plate Setup PCR Well type Unknown Standard NTC Collect fluorescence data Reference dye ROX Reference dye Assign Assay Name Well information Standard quantity Standard Replicate symbol Replicate Import Quick Setup Mx3000P & Mx3005P Real-Time QPCR System 93
95 Run Plate Setup Read properties RUN Start Run Pre-Read Post-Read Pre-Read Post-Read Mx3000P & Mx3005P Real-Time QPCR System 94
96 Raw data plot Raw data plot Raw data Raw data Show Plots Mx3000P & Mx3005P Real-Time QPCR System 95
97 Analysis Selection/Setup 1 Shift Ctrl 2 individually collectively Adv.Algorithm Settings Analysis Results Analysis Selection/Setup Plate sample values Standard curve Initial template quantity Dual color scatter plot Final call results Text report Consolidated reports Plate sample values well Ct Standard curve Standard well Ct Unknown well Mx3000P & Mx3005P Real-Time QPCR System 96
98 Dual color scatter plot well Ct Final call results well (+) (-) Final call Ct Ct (+) Text report Column Mx3000P & Mx3005P Real-Time QPCR System 97
99 Mx3000P/Mx3005P Allele discrimination/snp s Mx3000P/ Mx3005P QPCR TaqMan probe Allele discrimination / SNP s MxPro Real time PCR Advanced End point Allele discrimination/snp New Options Allele Discrimination / SNP s Real-Time Plate Read / Allele Discrimination Allele Discrimination / SNP s Real- Time TaqMan probe SNP/Mutation A/G SNP/Mutation SNP/Mutation TaqMan probe A T TaqMan probe FAM G C TaqMan probe VIC DNA PCR SNP/Mutation SNP/Mutation TaqMan probe A-homozygote FAM G-homozygote VIC heterozygote FAM VIC Dual color plot Negative control (NTC) NTC Mx3000P & Mx3005P Real-Time QPCR System 98
100 Allele Discrimination/SNP s Real-Time unknown: triplicate FAM VIC TaqMan probe SNP MxPro SNP/Mutation NTC (No template control) NTC FAM probe VIC probe SNP NTC FAM VIC Mx3000P/Mx3005P VIC HEX HEX VIC 20µl 3 Sample # Well type Filter Description Well #1 VIC positive FAM/VIC VIC positive cont. A1-C1 #2 FAM positive FAM/VIC FAM positive cont D1-F1 #3 unknown FAM/VIC Patient #1 A2-C2 #4 unknown FAM/VIC Patient #2 D2-F2 #5 unknown FAM/VIC Patient #3 A3-C3 #6 unknown FAM/VIC Patient #4 D3-F3 #7 unknown FAM/VIC Patient #5 A4-C4 #8 unknown FAM/VIC Patient #6 D4-F4 #9 NTC FAM/VIC No template A5-C5 SNP PCR 96 well plate / tubes Mx3000P / Mx3005P MxPro Mx3000P & Mx3005P Real-Time QPCR System 99
101 Allele Discrimination / SNP s Real-Time OK VIC positive (Allele 1) FAM positive (Allele 2) Unknown NTC Plate setup a) VIC HEX HEX VIC Optics Configration Mx3000P & Mx3005P Real-Time QPCR System 100
102 HEX-JOE Filter HEX VIC HEX VIC b) Well A1 C1 VIC positive c) Well A1 B1 C1 Well type VIC positive d) VIC FAM ROX ROX Reference dye Mx3000P & Mx3005P Real-Time QPCR System 101
103 e) D1 F1 FAM positive Well A2 F4 unknown Well A5 C5 NTC f) Replicate symbol Replicate Symbol Auto-increment Mx3000P & Mx3005P Real-Time QPCR System 102
104 g) Thermal profile Thermal profile Tm Thermal profile PCR h) Real-Time PCR Run Run PCR p55 Mx3000P & Mx3005P Real-Time QPCR System 103
105 Plate Read / Allele Discrimination SNP PCR 96 well plate / tubes Mx3000P / Mx3005P MxPro Plate Read / Allele Discrimination OK VIC positive (Allele 1) FAM positive (Allele 2) Unknown NTC Plate setup Plate Setup Allele Discrimination / SNP s Real-Time Plate Read Amplification plot End point Mx3000P/Mx3005P PCR Mx3000P/Mx3005P Thermal cycler Mx3000P & Mx3005P Real-Time QPCR System 104
106 Plate Read Mx3000P/Mx3005P Run Pre-Read Post-Read Mx3000P & Mx3005P Real-Time QPCR System 105
107 MxPro SNP MxPro Allele Discrimination /SNP s Allele Discrimination / SNP s Real-Time Plate Read / Allele Discrimination Plate Read / Allele Discrimination Allele Discrimination / SNP s Real-Time Amplification plot Area to analyze Mx3000P & Mx3005P Real-Time QPCR System 106
108 Allele Discrimination / SNP s Real Time Amplification plot Real-Time PCR a) Analysis Selection/Setup b) Results Amplification plot threshold line Results Amplification plot Amplification plot FAM VIC amplification plot FAM amplification plot FAM VIC homozygote FAM- Negative Threshold line Mx3000P & Mx3005P Real-Time QPCR System 107
109 negative VIC amplification plot FAM homozygous VIC negative negative threshold line Threshold line FAM VIC amplification plot VIC FAM c) Dual color plot Mx3000P & Mx3005P Real-Time QPCR System 108
110 Dual color plot amplification plot Last cycle Last cycle allele discrimination Dual color plot Amplification plot Dual color plot Amplification plot Show Dual clor plor line Last cycle Plate Read / Allele Discrimination Plate Read / Allele Discrimination experiment type SNP PCR SNP Real-time Negative threshold line End point Dual color plot Real-time PCR End point Mx3000P & Mx3005P Real-Time QPCR System 109
111 Dual color plot Real-Time PCR Plate Read Dual Color Scatter Plot Dual Color Scatter Plot allele Dual Color Scatter Plot FAM TET FAM TET FAM TET A allele FAM G allele TET A homozygote G homozygote heterozygote wild-type mutant-type Dual color plot plot Allele discrimination positive control NTC plot Mx3000P & Mx3005P Real-Time QPCR System 110
112 Plate sample value Real-Time Last cycle Dual color plot Amplification Plot Display the values for Ct Plate Read Fluorescence Rpost Rpre Dual color scapter plot Genotype Rectangle Mx3000P & Mx3005P Real-Time QPCR System 111
113 Allele associations Rename Alleles/Genotypes Allele Rename Alleles/Genotypes Dual color scatter plot Genotype rectangle Rectangle Reset genotype Rectangle Show all genotype Genotype Mx3000P & Mx3005P Real-Time QPCR System 112
114 confidence Final Call Result VIC FAM NTC positive Text Report Final Call Mx3000P & Mx3005P Real-Time QPCR System 113
115 Allele Discrimination / SNP s Real-Time Experiment Type Molecular Beacon probe Molecular beacon SNP allelic composition allele Molecular beacon allele molecular beacon A/G SNP A G Allele A allele molecular beacon Molecular beacon (MB) Molecular beacon + matched oligo (MBO) Molecular beacon + mismatched oligo (MBMO) Molecular beacon Molecular Beacon Melting Curve Experiment Type MBO MBMO MB MBO Molecular beacon 65 Molecular beacon MBMO Molecular beacon SNP MBO 50 Mx3000P & Mx3005P Real-Time QPCR System 114
116 SNP 60 MBO MBMO MBO MBMO 55 PCR threshold line Minimum Ct Spread algorithm MBMO amplification plot MBMO Ct MBO MBMO SNP allele Molecular beacon Molecular beacon FAM + FAM-MBO TET-MBO TET + FAM-MBMO TET-MBMO Mx3000P & Mx3005P Real-Time QPCR System 115
117 FAM TET FAM-MBMO TET-MBMO threshold line FAM TET Molecular beacon Ct FAM A-allele TET G-allele Molecular beacon heterozygote FAM+ heterozygote TET+ Heterozygote Molecular beacon unknown Threshold line Mx3000P & Mx3005P Real-Time QPCR System 116
118 FAM positive(homozygote) TET positive(homozygote) Heterozygote Dual Color Plot FAM-homozygote Heterozygote Negative TET-homozygote Amplification plot SNP endpoint amplification plot Molecular beacon Melting curve MxPro Mx3000P & Mx3005P Real-Time QPCR System 117
119 Molecular Beacon Melting Curve Analysis Quick Protocol Analysis Selection/Setup 1 Shift Ctrl 2 Select data collection ramp/plateau 3 individually collectively Adv.Algorithm Settings 4 Results 5 Area to analyze Annealing range (MBO) (MBMO) Melting curves/temperature Tm Mx3000P & Mx3005P Real-Time QPCR System 118
120 Beacon Designer or Beacon Designer Windows Mac CD Mac MacOSX safari MS IE Mx3000P & Mx3005P Real-Time QPCR System 119
121 Mx3000P Mx3005P Cat# Mx3000P Real-Time PCR System Bulb 48, Mx3000P / Mx3005P OFF Mx3000P / 3005P Mx3000P & Mx3005P Real-Time QPCR System 120
122 1) 2) 3) 4) 5) 6) 7) 8) 9) Lamp Reset and Utilities Mx3000P & Mx3005P Real-Time QPCR System 121
![Slope) ]-1 MxPro PCR](/docs-images/85/92531838/images/123-1.jpg "Mx3000P & Mx3005P Real-Time")
123 Dye and Quencher choice Efficiency = [10 (-1 / Slope) ]-1 MxPro PCR Mx3000P & Mx3005P Real-Time QPCR System 122
124 Stratagene s Mx3000P / Mx3005P Realtime QPCR system jtech@agilent.com Mx3000P & Mx3005P Real-Time QPCR System 123
プリント
Stratagene Products Mx3000P/Mx3005P PCR Mx3000P/Mx3005P Mx3000P PCR 5FRET Mx3005P PCR 401403 Mx3000P PCR 401449 Mx3005P PCR SYBR Green I TaqMan PCR PCRThreshold line ROX TM Tm 12Multiple Experimental Analysis)
AriaMx PCR AriaMx PCR PCR AriaMx PCR 0.2 ml 96 6 PCR Ct SYBR Green I PCR Tm TaqMan PCR DNA HRM TaqMan AriaMx 8 Multiple Experiment Analysis PDF RDML P
AriaMx PCR See Deeper. Reach Further. AriaMx PCR AriaMx PCR PCR AriaMx PCR 0.2 ml 96 6 PCR Ct SYBR Green I PCR Tm TaqMan PCR DNA HRM TaqMan AriaMx 8 Multiple Experiment Analysis PDF RDML PCR ROX Rn MIQE
untitled
January 2009 Rotor-Gene Q Sample & Assay Technologies 1 1-1 1.1 1-1 1.2 Rotor-Gene Q 1-1 1.3 Rotor-Gene Q 1-1 1.4 1-1 2 2-1 2.1 Run File 2-1 2.2 2-2 2.3 2-2 2.4 PCR 2-3 2.5 2-3 2.6 2-4 2.7 2-4 2.8 2-5
RT-PCR プロトコール.PDF
Real -Time RT-PCR icycler iq Bio Rad RT-PCR RT-PCR 1 icycler iq Bio Rad icycler iq 30 2 Ready-To-Go T-Primed First-Strand Kit (amersham pharmacia biotech) Ready-To-Go T-Primed First-Strand Kit QuantiTect
steponeplus_bro_f-0210.indd
StepOnePlus / StepOne PCR PCR Applied Biosystems StepOnePlus StepOne PCR 1 Features At A Glance StepOne StepOnePlus / 48 96 FAM/SYBR Green dyes VIC/JOE dyes ROX dyes NED/TAMRA dyes 1. VeriFlex Block StepOnePlus
steponeplus_bro_f-0912.indd
StepOnePlus /StepOne PCR PCR PCR StepOnePlus StepOne PCR PCR Features At A Glance StepOne StepOnePlus / 48 96 FAM /SYBR Green dyes VIC /JOE dyes ROX dyes 1. NED /TAMRA dyes VeriFlex Block StepOnePlus StepOne
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K - 18 - [1] 1 [2] 2 [3] 3 [4] 5 [5] : RNA cdna 13 [6] 14 [7] 17 LightCycler TM Idaho
ｽﾗｲﾄﾞ ﾀｲﾄﾙなし
LightCycler Software Ver.3.5 : 200206 1/30 Windows NT Windows NT Ctrl + Alt + Delete LightCycler 3 Front Screen 2/30 LightCycler3 Front RUN Data Analysis LightCycler Data Analysis Edit Graphics Defaults
steponeplus_bro_f-1124.indd
PCR StepOnePlus /StepOne PCR PCR PCR StepOnePlus StepOne PCR PCR Features At A Glance StepOne StepOnePlus / 48 96 FAM /SYBR Green dyes VIC /JOE dyes ROX dyes 1. NED /TAMRA dyes VeriFlex Block StepOnePlus
CycleavePCR® 呼吸器系感染症起因ウイルス検出キット Ver.2（製品コード CY216） 補足
CycleavePCR 呼吸器系感染症起因ウイルス検出キット Ver.2( 製品コード CY216) 補足 < Applied Biosystems StepOnePlus Real-Time PCR System の操作方法 > 詳細は 装置に付属の説明書をご確認ください (1)Experiment Properties 画面の設定を行う Experiment Type:Quantification-Standard
Cycleave®PCR 呼吸器系感染症起因菌検出キットVer.2（製品コード CY214） 補足
Cycleave PCR 呼吸器系感染症起因菌検出キット Ver.2( 製品コード CY214) 補足 < Applied Biosystems StepOnePlus Real-Time PCR System の操作方法 > 詳細は 装置に付属の説明書をご確認ください (1)Experiment Properties 画面の設定を行う Experiment Type:Quantification-Standard
PCR 3 Mature mirna PCR 7 miscript mirna PCR Array C T 12 Mature mirna cdna 15 miscript mirna QC PCR Array miscript mirna PCR Array 10/2011
October 2011 miscript mirna PCR Array miscript II RT Kit miscript SYBR Green PCR Kit miscript mirna PCR Arrays miscript mirna QC PCR Array Pathway-Focused mirna PCR Array mirnome miscript mirna PCR Array
SDS099-B1603OB_QuantStudio-3-and-5_PB_12.indd
QuantStudio 3 / QuantStudio 5 PCR PCR Applied Biosystems QuantStudio 3 QuantStudio 5 PCR Thermo Fisher Cloud 1 QuantStudio 3 and 5 Real-Time PCR Systems QuantStudio 3 and 5 Real-Time PCR Systems 2 Applied
PG1501-PJ7816-CO36363-QuantStudio-3-and-5-brochure-Global-J.indd
Applied Biosystems QuantStudio 3 / QuantStudio 5 PCR PCR Applied Biosystems QuantStudio 3 QuantStudio 5 PCR Thermo Fisher Cloud 1 QuantStudio 3 and 5 Real-Time PCR Systems QuantStudio 3 and 5 Real-Time
3 1 2
Agilent 4200 TapeStation Agilent 4200 TapeStation 3 1 2 2 3 ScreenTape 3 Agilent 4200 TapeStation QC Agilent 4200 TapeStation Ready-to-use ScreenTape 8 PCR 96-well plate 1 96 1 qrt-pcr DNA RNA DNA / RNA
PCR & PCR QuantStudio
PCR & PCR QuantStudio QuantStudio d PCR igital q uantitative PCR 1 PCR PCR SNP Genotyping PCR 1 1 DNA PCR PCR PCR PCR Applied Biosystems QuantStudio PCR PCR PCR PCR 20,000 5 log 1.1 NGS PCR / 10 log SNP
Step 1 Feature Extraction Featuer Extraction Feature Extraction Featuer Extraction Image Analysis Start>Programs>Agilent-Life Sciences>Feature Extract
Agilent G2565AA Feature Extraction Step 1 Feature Extraction Step 2 Step 3 Step 4 ( ) Step 5 ( ) Step 6 Step 7 Step 8 Feature Extraction Step 9 Step 10 Feature Extraction Step 11 Feature Extraction Step
(2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) 1 RT-PCR (10) (12) (13) F. Hoffmann-La Roche Ltd. Roche Molecular
05-8 SYBR Green Realtime PCR Master Mix (Code No. QPK-201, QPK-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3314K (2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux
untitled
PCR Enzymes & Instruments PCR Stratagene Gradient Cycler Problem PCRDNA Polymerase Solution Stratagene Gradient Cycler PCRQuikChange 3 Stratagene Gradient Cycler 96 96 Fast 384 3 3 0.4 0.1 12 96 Fast 384
(2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) (10) (12) F. Hoffmann-La Roche Ltd. Roche Molecular Systems Inc.
06-08 SYBR Green Realtime PCR Master Mix -Plus- (Code No. QPK-211, 211T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN (2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene
ProVisionaire Control V3.0セットアップガイド
ProVisionaire Control V3 1 Manual Development Group 2018 Yamaha Corporation JA 2 3 4 5 NOTE 6 7 8 9 q w e r t r t y u y q w u e 10 3. NOTE 1. 2. 11 4. NOTE 5. Tips 12 2. 1. 13 3. 4. Tips 14 5. 1. 2. 3.
Agilent Microarray Total Solution 5 5 RNA-Seq 60 mer DNA in situ DNA 5 2 QC 4200 TapeStation 2100 / mirna CGHCGH+SNP ChIP-on-chip 2 mirna QC
Microarray Agilent Microarray Total Solution Agilent Microarray Total Solution 5 5 RNA-Seq 60 mer DNA in situ DNA 5 2 QC 4200 TapeStation 2100 / mirna CGHCGH+SNP ChIP-on-chip 2 mirna QC RNA / mirna total
GeneMapperR Software Version 4.0 マイクロサテライト解析 Getting Started Guide
GeneMapper v4.0 Getting Started Guide GeneMapper v4.0 Getting Started Guide Copyright 2005, Applied Biosystems.All rights reserved. Applied Biosystems Applied Biosystems : Applera Corporation GeneMapper
PCR Rotor-Gene Q PCR DN QIGEN PCR Rotor-Gene Q QIGEN Rotor-Gene Q Rotor-Gene Q PCR High Resolution Melt SNP QIGEN Rotor-Gene Q QIGEN PCR HRM High Reso
Rotor-Gene Q PCR Sample to Insight PCR Rotor-Gene Q PCR DN QIGEN PCR Rotor-Gene Q QIGEN Rotor-Gene Q Rotor-Gene Q PCR High Resolution Melt SNP QIGEN Rotor-Gene Q QIGEN PCR HRM High Resolution Melt DN 8
PG1571-PJT1363-COL01356-MP T indd
Marketplace Thermo Scientific ALL PCR DNA メ ー PCR 製品 dntp PCR ー ン キ Essentials Tour 2016 キャンペーン ー DNA ー 製キ 2016 年 7 月 1 日 2016 年 9 月 30 日 まで キャンペーンの はこちらをご覧ください www.thermofisher.com/jp-mktp PCR Thermo
(2) (2) (3) (3) (4) ABI PRISM 7700 TaqMan (4) Roche LightCycler TM (5) BioFlux LineGene TaqMan (6) 1 RT-PCR (7) (9) (10) F. Hoffmann-La Roche Ltd. Roc
05-8 Realtime PCR Master Mix (Code No. QPK-101, QPK-101T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3313K (2) (2) (3) (3) (4) ABI PRISM 7700 TaqMan (4) Roche LightCycler TM (5) BioFlux LineGene
Specview Specview Specview STSCI(Space Telescope SCience Institute) VO Specview Web page htt
Specview Specview Specview STSCI(Space Telescope SCience Institute) VO Specview Web page http://www.stsci.edu/resources/software_hardware/specview http://specview.stsci.edu/javahelp/main.html Specview
GeneMapper. Software Version 4.0 LOH Analysis Getting Started Guide (PN 4363081 Rev. B)
GeneMapper v4.0 SNaPshot Kit SNP Getting Started Guide Panel Bin Set Primer Focus Kit Panel Bin Set SNaPshot Kit GeneMapper v4.0 SNaPshot Kit SNP Getting Started Guide Panel Bin Set Primer Focus Kit Panel
Microsoft Word - MetaFluor70取扱説明.doc
MetaFluor (Version 7.7) MetaFluor 1. MetaFluor MetaFluor Meta Imaging Series 7.x Meta Imaging Series Administrator CCD Meta Imaging Series Administrator CCD Molecular Devices Japan KK/ Imaging Team (1/14)
ProVAL Recent Projects, ProVAL Online 3 Recent Projects ProVAL Online Show Online Content on the Start Page Page 13
ProVAL Unit System Enable Recording Log Preferred Language Default File Type Default Project Path ProVAL : Unit SystemUse SI Units SI SI USCS Enable Recording Log Language Default File Type Default Project
フレキシブルな蛍光検出モジュールと優れた定量性能 信頼の AriaMx リアルタイム PCR システム AriaMx リアルタイム PCR システムは 独創的なオプティカルモジュール方式による高いフレキシビリティ 直感的に使えるタッチスクリーン 正確な定量解析を簡単に行える 解析用ソフトウェア シス
アジレント テクノロジー AriaMx リアルタイム PCR システム フレキシブルな蛍光検出モジュールと優れた定量性能 信頼の AriaMx リアルタイム PCR システム AriaMx リアルタイム PCR システムは 独創的なオプティカルモジュール方式による高いフレキシビリティ 直感的に使えるタッチスクリーン 正確な定量解析を簡単に行える 解析用ソフトウェア システムの異常を早期に発見できる自己診断機能を持ち合わせており
LSM5Pascal Ver 3.2 GFP 4D Image VisArt Carl Zeiss Co.,Ltd.
LSM5Pascal Ver 3.2 GFP 4D Image VisArt 2004.03 LSM5PASCAL V3.2 LSM5PASCAL SW3.2Axiovert200M 1 1 2 3 3 4 4 5 SingleTrack 9 Multi Track 10,18 5 / 21 6 3 27 7 35 8 ( OFF) 40 LSM5PASCAL V3.2 LSM5PASCAL 65
IonTorrentPGM_appnote_0319.indd
Ion PGM Heterogeneity * 1 * 1 * 2 * 2 * 2 * 2 * 2 * 1 *1 * 2 DNA DNA DNA ArcturusXT LCM Laser Capture Microdissection LCM A Workflow for Cancer Profiling Applied Biosystems ViiA7 PCR qpcr QC Assay Ion
0.2 Button TextBox: menu tab 2
Specview VO 2012 2012/9/27 Specview Specview STSCI(Space Telescope SCience Institute) VO Specview Web page http://www.stsci.edu/resources/software hardware/specview http://specview.stsci.edu/javahelp/main.html
Rotem Meter View Software
Rotem Meter View (RMV) Version 2.05 Rotem Meter View Software PRIR42X9.DOC Page 1 1... 3 2... 3 3... 3 4... 4 5... 4 5.1 PC COM... 4 5.2 Excel... 5 5.3... 5 5.3.1... 5 5.3.2 Lost Contact Interval... 6
30 OFF Ion Torrent Ion Torrent Ion AmpliSeq Ion Chip Ion 314 Chip Kit v2 BC 8 pack , ,000 Ion 316 Chip Kit v2 BC 8 pack
Applied Biosystems / Ion Torrent 2017 DNA 20 OFF DNA BigDye Terminator v3.1 Cycle Sequencing Kit 100 4337455 154,000 123,200 1,000 4337456 1,232,000 985,600 BigDye Terminator v1.1 Cycle Sequencing Kit
QIAzol Lysis Reagent MaXtract High Density RNAlater RNA Stabilization Reagent QIAGEN QIAzol Lysis Reagent RNA RNase MaXtract High Density QIAzol QIAzo
Vol.7November 216 3 DNA QIAamp DNA Mini Kit QIAcube QIAamp DNA Mini Kit 2 M Lung Kidney Liver Skin and muscle Whole blood Buffy coat Lymphocytes M QIAcube DNA 48.5 kb QIAamp DNA Mini Kit (5) 5134 25, QIAamp
1 I EViews View Proc Freeze
EViews 2017 9 6 1 I EViews 4 1 5 2 10 3 13 4 16 4.1 View.......................................... 17 4.2 Proc.......................................... 22 4.3 Freeze & Name....................................
CM1-GTX
CM1-GTX000-2002 R R i R ii 1-1 1-2 1-3 Process Variables Process Variables Pressure Output Analog Output Sensor Temp. Lower Range Value (0%) Upper Range Value (100%) Pressure Pressure Chart Pressure
New version (2.15.1) of Specview is now available Dismiss Windows Specview.bat set spv= Specview set jhome= JAVA (C:\Program Files\Java\jre<version>\
![New version (2.15.1) of Specview is now available Dismiss Windows Specview.bat set spv= Specview set jhome= JAVA (C:\Program Files\Java\jre<version>\](/thumbs/92/109450602.jpg "New version (2.15.1) of Specview is now available Dismiss Windows Specview.bat set spv= Specview set jhome= JAVA (C:\Program Files\Java\jre<version>\")
Specview VO 2012 2012/3/26 Specview Specview STSCI(Space Telescope SCience Institute) VO Specview Web page http://www.stsci.edu/resources/software hardware/specview http://specview.stsci.edu/javahelp/main.html
NanoVuePlus_H1-4_CC2014.indd
NanoVue Plus 71-3327-34 ... 2... 2... 2... 3... 4... 5... 6 NanoVue Plus...6 NanoVue Plus Sample... 7 Life Science... 9 1. DNA/RNA... 10 2. CyDye... 12 3.... 15 3-1Protein UV UV Protein A280 A280... 15
BRADY Worldwide, Inc. BRADY BRADY BRADY BMP 51 BRADY Worldwide, Inc. Microsoft Windows Microsoft BRADY 2011 BRADY Worldwide, Inc. All Rights Reserved
BRADY Worldwide, Inc. BRADY BRADY BRADY BMP 51 BRADY Worldwide, Inc. Microsoft Windows Microsoft BRADY 2011 BRADY Worldwide, Inc. All Rights Reserved 2011 2 2 Brady Worldwide Inc. 6555 West Good Hope Road
NanoVue_H1-4.indd
NanoVue 71-2888-36 ... 2... 2... 2... 3... 4... 5... 6 NanoVue...6 NanoVue Sample... 7 Life Science... 9 1. DNA/RNA... 10 2. CyDye... 12 3.... 15 3-1Protein UV UV Protein A280 A280... 15 3-2BCABradford
HA8000シリーズ ユーザーズガイド ～BIOS編～ HA8000/RS110/TS10 2013年6月～モデル
P1E1M01500-3 - - - LSI MegaRAID SAS-MFI BIOS Version x.xx.xx (Build xxxx xx, xxxx) Copyright (c) xxxx LSI Corporation HA -0 (Bus xx Dev
150602_AN_SureSelect と KAPA Hyper Prep.indd
Agilent SureSelect KAPA Hyper Prep Kit DNA 10 ng : FFPE DNA DNA V2.5BJ 2015 5 & : KAPA Hyper Prep Kit SureSelect XT :KAPA Hyper Prep Kit, SureSelect, Agilent SureSelect XT PCR Primer KAPA Hyper Prep Kit
5.2 White
1 EViews 1 : 2007/5/15 2007/5/25 1 EViews 4 2 ( 6 2.1............................................ 6 2.2 Workfile............................................ 7 2.3 Workfile............................................
リアルタイム PCR 用試薬 Luna Universal qpcr & RT-qPCR be INSPIRED drive DISCOVERY stay GENUINE
リアルタイム PCR 用試薬 Luna Universal qpcr & RT-qPCR be INSPIRED drive DISCOVERY stay GENUINE 用途に最適な qpcr 試薬を提供 -Step RT-qPCR 2-Step RT-qPCR RNA RNA 逆転写 cdna cdna 逆転写 & qpcr Luna Universal One-Step RT-qPCR Kit
AUTO-TUNE 4
AUTO-TUNE 4 Chapter 1 4 Auto Tune4.0 4 5 7 Chapter 2 Auto-Tune 4 8 8 9 MIDI 12 15 Chapter 1 Auto-Tune Auto-Tune 4 Auto-Tune 4.0 Auto-Tune 4 Auto-Tune 4 Auto-Tune 4 192kHz Options Scale Note Remove Bypass
ThermoFisher
Thermo Fisher Connect Relative Quantification 操作簡易資料 http://www.thermofisher.com/cloud 使用には事前登録が必要になります 画面は予告なく変わることがあります The world leader in serving science Thermo Fisher Connect とは? キャピラリシーケンサ リアルタイム
TM-m30 詳細取扱説明書
M00094106 Rev. G Seiko Epson Corporation 2015-2018. All rights reserved. 2 3 4 5 6 7 8 Bluetooth 9 ... 71 10 1 11 Bluetooth 12 1 13 1 2 6 5 4 3 7 14 1 1 2 3 4 5 15 16 ONF 1 N O O N O N N N O F N N F N
Introduction Purpose This training course demonstrates the use of the High-performance Embedded Workshop (HEW), a key tool for developing software for
Introduction Purpose This training course demonstrates the use of the High-performance Embedded Workshop (HEW), a key tool for developing software for embedded systems that use microcontrollers (MCUs)
ワイヤレス～イーサネットレシーバー UWTC-REC3
www.jp.omega.com : esales@jp.omega.com www.omegamanual.info UWTC-REC3 www.jp.omega.com/worldwide UWIR UWTC-NB9 / UWRH UWRTD UWTC 61.6 [2.42] REF 11.7 [0.46] 38.1 [1.50] 66.0 [2.60] REF 33.0 [1.30]
Microsoft Word - PIVマニュアル.doc
(Nikkor 50mm f/1.2) C CCD (PixelFly QE) LAN USB BNC 1 1.1 CCD 注意 CCD CCD 1) 注意 2) 3) LAN LAN 4) 3 2 5) 2 1.2 1) Came Ware Came Ware 2) [Camera] [Camera Control] Camera mode Video Trigger Mode Intern CameraControl
GeneMapper. Software Version 4.0 AFLP. System Analysis Getting Started Guide (PN )
GeneMapper v4.0 AFLP Getting Started Guide GeneMapper v4.0 AFLP Getting Started Guide Copyright 2005 Applied Biosystems Japan Ltd. All rights reserved. Applied Biosystems Applied Biosystems : Applera
目 次
YS20060216 ...1 1 VP-ITC...3.....5. VP-ITC. VP-ITC. -...8.........22......25.....32. Y-Axis...34.......37 ii 10-12 V( ) VP-ITC 150cm 75cm NMR LAN VP-ITC VP-ITC 100VOA 3 VP-ITC 1 VP-ITC *.itc Origin *.opje-mail
PG1571-PJT1690-COL02509-MP T2_J.indd
molecular biology Marketplace MOLECULAR BIOLOGY GREATEST HITS IPTG X-Gal DNA DNA PCR dntp Thermo Scientific off Essentials Tour 2016 2016 10 3 2016 12 22 www.thermofisher.com/marketplace Thermo Scientific
TM-m30 詳細取扱説明書
M00094100 Rev. A Seiko Epson Corporation 2015. All rights reserved. 2 3 4 5 6 Bluetooth 7 Bluetooth 8 1 9 Bluetooth 10 1 11 1 2 6 5 4 3 7 12 1 13 14 ONF 1 N O O N O N N N O F N N F N N N N N N F F O O
Nios II ハードウェア・チュートリアル
Nios II ver. 7.1 2007 8 1. Nios II FPGA Nios II Quaruts II 7.1 Nios II 7.1 Nios II Cyclone II count_binary 2. 2-1. http://www.altera.com/literature/lit-nio2.jsp 2-2. Nios II Quartus II FEATURE Nios II
1 1.1 (JCPRG) 30 Nuclear Reaction Data File (NRDF) PC GSYS2.4 JCPRG GSYS2.4 Java Windows, Linux, Max OS X, FreeBSD GUI PNG, GIF, JPEG X Y GSYS2
(GSYS2.4) GSYS2.4 Manual SUZUKI Ryusuke Hokkaido University Hospital Abstract GSYS2.4 is an update version of GSYS version 2. Main features added in this version are Magnifying glass function, Automatically
UM PM Q24 ( /2013)
July 2011 PyroMark Q24 Sample & Assay Technologies 1 User Manual 1 Safety Information 2 User Manual 2 Introduction 3 User Manual 3 General Description 4 4 4.1 4 4.2 4 5 5 5.1 5 5.1.1 5 5.1.2 5 5.1.3 5
Cleaner XL 1.5 クイックインストールガイド
Autodesk Cleaner XL 1.5 Contents Cleaner XL 1.5 2 1. Cleaner XL 3 2. Cleaner XL 9 3. Cleaner XL 12 4. Cleaner XL 16 5. 32 2 1. Cleaner XL 1. Cleaner XL Cleaner XL Administrators Cleaner XL Windows Media
TM-m30 詳細取扱説明書
M00094101 Rev. B Seiko Epson Corporation 2015-2016. All rights reserved. 2 3 4 5 6 7 8 Bluetooth 9 Bluetooth 10 1 11 Bluetooth 12 1 13 1 2 6 5 4 3 7 14 1 1 2 3 4 5 15 16 ONF 1 N O O N O N N N O F N N F
RNA_Brochure_2012JPCS55_0913.indd
RNA produced by RNA Ambion RNA Ambion Confidence comes naturally Ambion 20RNA RNA RNA 2 4 6 Total RNA 12 mrna 14 18 mirna 20 RNase 22 RNA 3 RNA Ambion RNA RNA RT-PCR qpcr RNA RNA PureLink RNA Mini Kit
フリーセルプロの使い方
FreeCell Pro 011 2 FreeCell Pro 2002 FCPRO.HLP FreeCell Pro6.4 6.5 FreeCell Pro 1000 http://solitairelaboratory.com/fcpro.html FreeCell Pro 2009 2 3 FreeCell Pro Microsoft FC 0 Windows 3.1 FreeCell Pr
C
(1) (2) Play Volume (3) (4) (5) (6) (7) (8) Ctrl N Ctrl Ctrl (9) (10) OFF (11) (12) OFF (13) (14) 2 3 4 5 6 7 LCD LCD 8 TV ANT. S IN VIDEO IN LINE-L IN LINE-R IN 9 10 11 12 LCD LCD 13 LCD 14 LCD 15 LCD
BRC-X1000
C-455-100-02(1) BRC-X1000/H800 2016 Sony Corporation 2 3 4 5 6 4 D E 5 6 7 A B C F G 7 8 9 0 qa qs SYSTEM SELECT IN IR SELECT VISCA RS - 422 OUT OSD OFF CAMERA SETUP 1 qd 2 3 4 5 6 7 8 LAN 12V qf HDMI
untitled
2008/02/05 Isoiso FACSCalbur 488nm FL1 530/30 FITC GFP alexa488 FL2 585/42 PE Cy3 PI FL3 650LP Percp PC5 PI PE-C 7 Red APC Falcon BD Calibrite 3 BD No.340486 DNA QC particles BDNo.349523 Flow-Cheak(10um)
PrimerArray® Analysis Tool Ver.2.2
研究用 PrimerArray Analysis Tool Ver.2.2 説明書 v201801 PrimerArray Analysis Tool Ver.2.2 は PrimerArray( 製品コード PH001 ~ PH007 PH009 ~ PH015 PN001 ~ PN015) で得られたデータを解析するためのツールで コントロールサンプルと 1 種類の未知サンプル間の比較が可能です
GeneMapper® Software Version 4.0 LOH 解析 ユーザーガイド
GeneMapper v4.0 LOH Getting Started Guide LOH (Loss of Heterozygosity) LOH (Loss of Heterozygosity) GeneMapper v4.0 LOH Getting Started Guide LOH (Loss of Heterozygosity) LOH (Loss of Heterozygosity)
untitled
TEM with CCD Ver. 5.1 18 10 25 6 50 FILAMENT OFF 80 V () FILAMENT 2 25 FILAMENT OFF FILAMENT 1 min 2-5 L TEM(&CCD) FILAMENT ON CCD( ) - 1 - 3 TEM 3 CCD 3 4 5 6 7 (CCD ) 7 CCD 7 10 10 11 CCD &TEM 11-2 -
HA8000-bdシリーズ RAID設定ガイド HA8000-bd/BD10X2
HB102050A0-4 制限 補足 Esc Enter Esc Enter Esc Enter Main Advanced Server Security Boot Exit A SATA Configuration SATA Controller(s) SATA Mode Selection [Enabled] [RAID] Determines how
Introduction Purpose This training course describes the configuration and session features of the High-performance Embedded Workshop (HEW), a key tool
Introduction Purpose This training course describes the configuration and session features of the High-performance Embedded Workshop (HEW), a key tool for developing software for embedded systems that
グラフ数値読み取りシステム (GSYS2.4) 利用の手引
(GSYS2.4) GSYS2.4 Manual SUZUKI Ryusuke Hokkaido University Hospital 2011 6 7 Abstract GSYS2.4 is an update version of GSYS version 2. Main features added in this version are Magnifying glass function,
TOPLON PRIO操作手順
TOPLON PRIO 2004/05/24 I/O LON WAGO TOPLON PRIO 1. 1) PCC-10 S/W 2) PC 3) PCC-10 4) Windows Lon WorksR Plug n Play Apply OK 5) Visio LonMaker LonPoint 6) TOPLON PRIO 2. IO-PRO SYM TOPLON-PRIO SNVT NVI
ECCS. ECCS,. ( 2. Mac Do-file Editor. Mac Do-file Editor Windows Do-file Editor Top Do-file e
1 1 2015 4 6 1. ECCS. ECCS,. (https://ras.ecc.u-tokyo.ac.jp/guacamole/) 2. Mac Do-file Editor. Mac Do-file Editor Windows Do-file Editor Top Do-file editor, Do View Do-file Editor Execute(do). 3. Mac System
こんにちは由美子です
Sample size power calculation Sample Size Estimation AZTPIAIDS AIDSAZT AIDSPI AIDSRNA AZTPr (S A ) = π A, PIPr (S B ) = π B AIDS (sampling)(inference) π A, π B π A - π B = 0.20 PI 20 20AZT, PI 10 6 8 HIV-RNA
Sequencher 4.9 Confidence score Clustal Clustal ClustalW Sequencher ClustalW Windows Macintosh motif confidence Sequencher V4.9 Trim Ends Without Prev
2009 Gene Codes Corporation Gene Codes Corporation 775 Technology Drive, Ann Arbor, MI 48108 USA 1.800.497.4939 (USA) +1.734.769.7249 (elsewhere) +1.734.769.7074 (fax) www.genecodes.com info@genecodes.com
取扱説明書の読み替え一覧表
SCSI アレイコントローラカード取扱説明書 ( 追補版 ) PG-140BL PG-140C PG-140CL PG-141B PG-142B PG-142C PG-142D GP5-150 GP5-1501 GP5-151 はじめに Linux MicrosoftWindows NTMicrosoft Corporation NetwareNovell Copyright 1985-2001 Microsoft
untitled
PCRM-PCR PCR MPCR Dualreal PCR PCR (M-PCR) PCR (M-PCR) Q & A PCR DNA 50 2030 DNA PCR 1 PCR (MPCR) MBI PCR 1 MPCR 1 MPCR DNA 1 PCR 510 DNA (US patent 5, 582, 989) 1 DNA ( 1) MPCR GC MBI MPCR PCR DNA 2 MBI
QuantStudio_3D_Prod_Bulletin-JP-4th-0326.indd
3D PCR 3D PCR PCR 2, 3D PCR PCR 3D PCR PCR PCR PCR 1 PCR PCR Ct CNV mrna mirna GMO サンプル調製サンプルの分配 DNA の増幅結果 コピー数 / µl サンプルを多数の反応に分配します ポジティブ反応ネガティブ反応 1. PCRPCR 1 PCR PCR Ct 3D PCR PCR 3D PCR 1 /µl 2 3D
untitled
1 OrCAD PSpice OrCAD PSpice OrCAD PSpice OrCAD Capture OrCAD Capture OrCAD 15.7 Demo OrCAD Capture CIS Demo 1.1 Capture 1 OrCAD Capture 1.2 1.2 OrCAD Capture [File] [New] [Project] 1.3 Project 2 New Project
九州大学学術情報リポジトリ Kyushu University Institutional Repository リアルタイム PCR : ソノゲンリトトクチョウ 小島, 夫美子九州大学医学部保健学科検査技術科学専攻 大阪大学大学院医学系研究科保健学専攻 岩谷, 良則大阪大学大学院医学系研究科保健学
九州大学学術情報リポジトリ Kyushu University Institutional Repository リアルタイム PCR : ソノゲンリトトクチョウ 小島, 夫美子九州大学医学部保健学科検査技術科学専攻 大阪大学大学院医学系研究科保健学専攻 岩谷, 良則大阪大学大学院医学系研究科保健学専攻 藤本, 秀士九州大学医学部保健学科検査技術科学専攻 https://doi.org/10.15017/54
Microsoft Word - RMD_75.doc
Review Multi Dimensional Data Multi Dimensional Acquire *.nd MetaMorph.nd Muliti Dimensional Acquisition Stack Tiff Multi Dimensional Acquisition Z nd nd 1 Review Multi Dimensional Data nd Review Multi
Slide 1
Bioanalyzer と qpcr を使用したライブラリの定性 定量評価 酒井名朋子イルミナ株式会社テクニカルサポート 2010 Illumina, Inc. All rights reserved. Illumina, illuminadx, Solexa, Making Sense Out of Life, Oligator, Sentrix, GoldenGate, GoldenGate Indexing,
JAJP.indd
Agilent Application Note 1....1 2. MIMO...2 2.1...2 2.2 MIMO...3 3. Agilent MIMO...4 3.1 P...4 3.2 U2000 USB...7 4....10 4.1 P...11 4.2 U2000...16 2 T 0 T 1 = 1.15 0.26 0.39 1.03 R 0 R 1 CB.log 2 1 C MIMO
CycleavePCR™ Library Quantification Kit (for Illumina)
研究用 CycleavePCR Library Quantification Kit (for Illumina) 説明書 v201811da イルミナ社の次世代シーケンサー (MiSeq Genome Analyzer IIx HiScanSQ HiSeq 1000 HiSeq 2000) は 一度に多量の DNA 分子の塩基配列を超並列に決定します 通常 解析に必要なライブラリーは DNA 断片の両端に指定のアダプターを連結することにより調製されます
13B1X gonyx 13B1X gonyx 13B1X gonyx 13B1X
STRAUMANN CARES GUIDED SURGERY c odiagnostix 使用説明書 13B1X10163000172 gonyx 13B1X10163000173 gonyx 13B1X10163000174 gonyx 13B1X10163000175 1 1. 4 1.1 4 1.2 4 1.3 5 1.4 5 1.5 5 1.6 7 1.7 8 1.8 9 1.9 10 1.10
作業手順手引き
Praat Introduction to Praat: Let's take a look at sounds : * WS Dec/01/'14 : ver. 1.1.4 1. Praat STEP 1: STEP 2: STEP 3: STEP 4: STEP 2 Editor STEP 3 Dynamic menu 2 FAQ: Pitch analysis http://goo.gl/r65la
/
333 344 / 332 03-6264-066003-6264-0663 03-6264-0620 334 LAL 335 336 LAL 338 QCL-1000 LAL 33 LAL 340 C 341 LAL 343 ELx808 344 FLx800 345 PyroTec 346 WinKQCL 347 CSE 348 / 03-6264-066003-6264-0663 03-6264-0620
untitled
Track Stick...1...2...7...8...9...10...10...14...14...17...19...23 1. CD CD 2. INSTALL TRACK SITCK MANAGER 3. OK 2 4. NEXT 5. license agreement I agree 6. Next 3 7. 8. Next 9. Next 4 10. Close 9 OK PDF
MACS機器予算申請カタログ
MACS Instruments MACSQuant automacs MultiMACS gentlemacs MACSmix TM MACSQuant 3 MACSQuant Analyzer 10 MACSQuant VYBMACSQuant X MACSQuant X Coming soon! 384 well plate Laser Channel Filter (nm) Dye or Parameter
Version1.5
Version1.5 Version Date Version1.0 Version1.1 Version1.2 Version1.3 Version1.4 Version1.5 Test J/K/SE0_NAK USB-IF Test Procedure FS Upstream Signal Quality Test Receiver Sensitivity Test DG2040 Packet
HARK Designer Documentation 0.5.0 HARK support team 2013 08 13 Contents 1 3 2 5 2.1.......................................... 5 2.2.............................................. 5 2.3 1: HARK Designer.................................
XPS分析のための簡易マニュアル
XPS 200.. XPS XPS XPS XPS 1. N2 0.4MPa 2. ON PUMP 3. POWER SUPPLY CONTROL UNIT SPEC ON 4. POWER SUPPLY CONTROL UNIT X-R ON 0.4MPa RESET 5. X-RAY POWER UNIT (a) AUTO/MANUMANU (b) Al/MgMg (c) FILAMENT ON/OFFOFF
mRNA-Seq_SamplePrep.book
mrna Sequencing Sample Preparation Guide Topics 3 Introduction 4 RNA Input Recommendations 6 mrna-seq Sample Preparation Kit Contents 8 User-Supplied Consumables and Equipment 9 Purify the mrna 12 Fragment
ＷｉｎＰＳＫユーザーズガイド
8 ae4jy@mindspring.com - 1 - - 2 - 5 5.. 5 5 5.. 6 6 1 1 1 1 1 1 1 1-3 - Status Bar 36-4 - Peter Martinez, WinPSK MHz Pentium Windows95,98 NT4.0 DX VGA640x480 Help MB RAM CPU WinPSK CPU CPU Too Slow CPU
PrimerArray Analysis Tool Ver.2.1
PrimerArray Analysis Tool Ver.2.1 説 明 書 PrimerArray Analysis Tool Ver.2.1 は PrimerArray ( 製 品 コード PH001 ~ PH010 PN001 ~ PN010) で 得 られたデータを 解 析 するためのツールで コントロールサンプルと 1 種 類 の 未 知 サンプル 間 の 比 較 が 可 能 です リアルタイム
Microsoft Word - Meta70_Preferences.doc
Image Windows Preferences Edit, Preferences MetaMorph, MetaVue Image Windows Preferences Edit, Preferences Image Windows Preferences 1. Windows Image Placement: Acquire Overlay at Top Left Corner: 1 Acquire
GM-F520S/GM-F470S/GM-F420S
GM-F520S GM-F470S GM-F420S LCT2504-002A-H 2 3 4 200 150 150 50 1 3 4 1 2 3 1 2 3 4 5 e 6 7 8 9 p q w r t 5 6 5 23 7 8 9 p q 4 5 6 7 8 9 2 3 4 5 5 23 6 7 8 9 w 1 2 e r t p p 5 6 9( 3 DVI-D (HDCP) RGB IN
untitled
SUBJECT: Applied Biosystems Data Collection Software v2.0 v3.0 Windows 2000 OS : 30 45 Cancel Data Collection - Applied Biosystems Sequencing Analysis Software v5.2 - Applied Biosystems SeqScape Software