PCR Rotor-Gene Q PCR DN QIGEN PCR Rotor-Gene Q QIGEN Rotor-Gene Q Rotor-Gene Q PCR High Resolution Melt SNP QIGEN Rotor-Gene Q QIGEN PCR HRM High Reso
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1 Rotor-Gene Q PCR Sample to Insight
2 PCR Rotor-Gene Q PCR DN QIGEN PCR Rotor-Gene Q QIGEN Rotor-Gene Q Rotor-Gene Q PCR High Resolution Melt SNP QIGEN Rotor-Gene Q QIGEN PCR HRM High Resolution Melt DN 8 12 mirn 2 Rotor-Gene Q 7/215
3 Rotor-Gene Q PCR 1 2 ±.2 ±.2 1/2 ROX Reaction chamber 7 2 Tubes in rotor spin past optics 3 Motor spindle assembly 4 4 LED lights 5 Lens 6 Emission filters 7 Photomultiplier detector Centrifugal fan drives cool air into chamber Vent opens to expel hot air Centrifugal fan drives air around chamber Heating element switches off 2. PCR DN 2 3 ng.6 ng 1, 2 PCR IL1R2 TaqMan Rotor-Gene Probe PCR Kit Rotor-Gene Q 5 replicate C T 1.7 CL2 TaqMan Rotor-Gene Probe PCR Kit Rotor-Gene Q ROX DN PCR 72 replicate C T CV.2 1. Rotor-Gene Q Rotor-Gene Q 7/215 3
4 Rotor-Gene Q SYR Green TaqMan FRET UV 6 1 Rotor-Gene Q 1. Rotor-Gene Q 72-Well Rotor nm nm lue 365 ± 2 46 ± 2 Marina lue, Edans, othell lue, lexa Fluor 35 Green 47 ± 1 51 ± 5 FM, SYR Green I, Fluorescein, EvaGreen, lexa Fluor 488 Yellow 53 ± ± 5 JOE, VIC, HEX, TET, CL Fluor Gold 54, Yakima Yellow Orange 585 ± 5 61 ± 5 ROX, CL Fluor Red 61, Cy 3.5, Texas Red, lexa Fluor 568 Red 625 ± 5 66 ± 1 Cy5, Quasar 67, Lightr Red64, lexa Fluor 633 Crimson 68 ± high pass Quasar 75, Lightr Red75, lexa Fluor 68 HRM 46 ± 2 51 ± 5 SYR Green I, SYTO9, LC Green, LC Green Plus+, EvaGreen Rotor-Gene Q Rotor-Gene Q PCR Rotor-Disc Rotor-Disc Rotor-Disc 72 Rotor-Dsic 72 1 Rotor-Disc 1 Rotor-Disc Rotor- Disc Heat Sealer QIgility PCR PCR QIgility Rotor-Gene Q QIgility PCR 4 Rotor-Gene Q 7/215
5 HRM High Resolution Melt HRM PCR PCR GC T m PCR T m High Resolution Melt HRM T m Rotor-Gene Q ±.2 PCR Rotor-Gene Q HRM Heterozygote Homozygote mutant Heterozygote Homozygote mutant Wild type Wild type Temperature ( C) Heterozygote 5 Rotor-Gene Q HRM Heterozygote 5 HRM Wild type ± Wild 84 type Temperature ( C) Temperature ( C) 3. HRM SNP PPP1R14 protein phosphatase 1, regulatory Rotor-Gene Q class IV SNP T HRM [inhibitor] subunit 14 SNP rs G DN 1 ng Type-it HRM PCR 1 PCR HRM Kit Rotor-Gene Q 9 GG G 8 1% methylated HRM wt 7 difference plot 3 6 9%. 7% %. 3 3%. 2 1%. 1 % methylated % methylated 7 6 9%. 7% 5 4 5%. 3 3%. 2 1%. 1 % methylated Temperature ( C) minus 5% meth Temperature ( C) Normalized fluorescence minus minus wt wt Temperature ( C) Temperature ( C) Homozygote mutant Homozygote mutant 4. HRM DN-PC adenomatosis polyposis coli EpiTect HRM PCR Kit Rotor-Gene Q HRM HRM 5 difference plot minus 5% meth Temperature ( C) Rotor-Gene Q 7/215 5
6 Rotor-Gene Q PCR 2 2. Rotor-Gene Q 2 C T Export to Relative Expression Software Tool REST Export to LinRegPCR assumption-free analysis Melt HRM Scatter Plot Virtual mode Rotor-Gene Q Rotor-Gene Q LED 6 Rotor-Gene Q 7/215
7 Rotor-Gene Q Rotor-Gene Q Rotor-Disc OTV Optical Temperature Verification Kit Rotor-Disc Rotor-Gene Q Rotor-Gene Q PCR 3 3. Rotor-Gene Q 37 mm x 42 mm x 286 mm 538 mm 12.5 kg ±.2 ±.5 ± nm nm LED Gain PMT 1 1 Rotor-Disc 1 3 µl x µl Rotor-Disc 72.1 ml x µl*.1 ml Strip Tubes.1 ml x µl PCR.2 ml.2 ml x µl QIGEN Rotor-Gene Kit V C 5/6 Hz 56 V peak *.1 ml Strip Tubes 2 µl SN Rotor-Gene Q 7/215 7
8 Rotor-Gene Q QIGEN PCR 4 5 PCR Q-ond 6 45 PCR Factor MP PCR Factor MP DN 4. QIGEN DN PCR SYR Green PCR 2 RT-PCR Rotor-Gene SYR Green PCR Kit 5 SYR Green 1 RT-PCR Rotor-Gene SYR Green RT-PCR Kit 7 singleplex PCR 2 RT-PCR Rotor-Gene Probe PCR Kit 2 singleplex 1 RT-PCR Rotor-Gene Probe RT-PCR Kit 8 multiplex PCR 2 RT-PCR Rotor-Gene Multiplex PCR Kit 9 mirn SYR Green 2 RT-PCR miscript SYR Green PCR Kit singleplex PCR 2 RT-PCR QuantiTect Virus +ROX Vial Kit 1 multiplex 1 RT-PCR SNP PCR Type-it Fast SNP Probe PCR Kit 11 EvaGreen HRM Type-it HRM PCR Kit 3 PCR EpiTect MethyLight PCR Kit 12 EvaGreen HRM EpiTect HRM PCR Kit 4 NH4 + K + NH3 + H + K + P _ H P _ 5. QIGEN PCR NH 4 + K + K + DN NH 4 + Fast 6. Rotor-Gene Master Mix Type-it Fast SNP PCR Master Mix Q-ond DN DN DN Q-ond 8 Rotor-Gene Q 7/215
9 Rotor-Gene SYR Green Kit QuantiTect Primer ssay Rotor-Gene Q QuantiTect Primer ssay GeneGlobe Rotor-Gene SYR Green Kit PCR df/dt QIGEN Temperature ( C) s 5. SYR Green RT-PCR R QIGEN II C T Mean deviation C T Mean deviation X CL2-associated X protein CL2 apoptosis gene MYC proto-oncogene b-ctin housekeeping gene cdn 1 ng SYR Green 2 RT-PCR 4 QuantiTect Primer ssay triplicate X CL2 MYC b-ctin Rotor-Gene Q Rotor-Gene SYR Green RT-PCR Kit II C T TaqMan Gene Expression ssays Rotor-Gene Probe Kit Rotor-Gene Q.3 1 RT-PCR 8 QIGEN II 7. SYR Green RN 1 1 ng 1 pg SYR Green 1 RT-PCR CL2 -cell CLL/lymphoma 2 QuantiTect Primer ssay duplicate Rotor-Gene Q Rotor-Gene SYR Green RT-PCR Kit 1 pg RN PCR R Mg 2+ 1 pg PCR 6 E Rn 5 E-1 4 E-1 3 E-1 2 E-1 1 E E E-1 RN 1 1 ng 1 ng 1 4 E-1RT-PCR IL12R1 interleukin 12 receptor, beta 1 TaqMan Gene Expression ssay3 E-1 Triplicate Rotor-Gene Q Rotor-Gene Probe RT-PCR Kit 2 E-1 II C T 1 E-1 Rn Rotor-Gene Q 7/215 9
10 Rotor-Gene Multiplex PCR Kit Rotor-Gene Q 4 cdn 9 C T C TNF tumor necrosis factor IFNG interferon, gamma MYC v-myc myelocytomatosis viral oncogene homolog [avian] HSP91 heat shock protein 9 kda alpha [cytosolic], class member 1 TNF FM Cy5 HEX Texas Red CT values D TNF N.D TNF IFNG IFNG MYC MYC HSP9 HSP IFNG cdn 1 1 ng 1 pg 4-plex PCR Rotor-Gene 2 Q Rotor-Gene Multiplex PCR Kit S triplicate.12 1 TaqMan 4 C T S IFNG.1 N.D. Rotor-Gene Q C T C TNF S.8 D IFNG S mirn mirn QIGEN 3 miscript PCR System mirn mrn cdn SYR Green PCR mirn miscript II Reverse Transcription Kit miscript SYR Green PCR Kit miscript Primer ssay Rotor-Gene Q mirn miscript PCR System 1 Rotor-Gene Q 7/215
11 -2. QuantiTect Virus +ROX Vial Kit Rotor-Gene Q 4 DN RN singleplex RN DN 1 QIGEN II influenza virus RN1 4 in vitro duplex.14 RT-PCR singleplex 1 RT-PCR TaqMan.12 triplicate Rotor-Gene Q QuantiTect Virus +ROX Vial Kit duplex.1 singleplex.8 II duplex.6 singleplex SNP Type-it Fast SNP Probe PCR Kit SNPs Rotor-Gene Q SNP 11 SNP TaqMan MG TaqMan 11. SNP Type-it Fast SNP Probe PCR Kit 7 DN 1 ng llelic discrimination rs TaqMan SNP 2 NTCs Rotor-Gene Q PCR NTCs FM T allele VIC allele Rotor-Gene Q 7/215 11
12 DN HRM 5 4 CpG EpiTect MethyLight PCR Kit Rotor-Gene Q TaqMan EpiTect MethyLight ssay EpiTect MethyLight ssay PCR 2 single PCR 12 excitation excitation C Primer 5' 3' excitation FM FM FM VIC Q Q excitation Primer.4 5'.3 3'.2 3' 5' Primer.1 Fluorophore 1 (FM). Fluorophore 2 (VIC) Quencher VIC Q D VIC.16 1%.14 9% % 1% % NTC VIC FM Q 1% 9% 5% 3' 5' Primer % NTC 45 1% % 1% 5% 9% 1% NTC 45 Q.16 C T CG FM.14 CG FM C T TG % VIC TG VIC 1% threshold C meth = 1/ C T CG C T TG.12threshold 5%.1.8 9%.6 C.4 T CG C T TG FM VIC.2 1% NTC EpiTect MethyLight PCR Kit DN 2 TaqMan PCR isulfite DN FM TaqMan isulfite DN VIC TaqMan DN PCR 1 ng isulfite DN DN 9 1 DN Hs_TMEFF2 EpiTect MethyLight ssay EpiTect MethyLight PCR Kit Rotor-Gene Q DN C T CG FM C T C DN C T TG VIC C T D 12 Rotor-Gene Q 7/215
13 Cat. no. Rotor-Gene Q 2plex System* Rotor-Gene Q 2plex HRM System* Rotor-Gene Q 5plex System* Rotor-Gene Q 5plex HRM System* Rotor-Gene Q 6plex System* Rotor-Disc 1 Starter Kit Rotor-Gene Q 2plex green yellow Rotor-Gene Q Software PC Rotor-Gene Q 2plexHRM green yellow HRM Rotor-Gene Q Software PC Rotor-Gene Q 5plex green yellow orange red crimson Rotor-Gene Q Software PC Rotor-Gene Q 5plex HRM green yellow orange red crimson HRM Rotor-Gene Q Software PC Rotor-Gene Q 6plex blue green yellow orange red crimson Rotor-Gene Q Software PC Rotor-Disc 1, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 1 Rotor and Locking Ring, Rotor-Disc 1 Loading lock, Rotor-Disc Pipetting id ,5, ,9, ,5, ,9, ,2, , Rotor-Disc 1 (3) Pack of 3 individually wrapped discs for 3 reactions ,5 Rotor-Disc 72 Starter Kit Rotor-Disc 72, Rotor-Disc Heat Sealer, Rotor-Disc Heat Sealing Film, Rotor-Disc 72 Rotor and Locking Ring, Rotor-Disc 72 Loading lock, Rotor-Disc Pipetting id , Rotor-Disc 72 (24) Pack of 24 individually wrapped discs for 1728 reactions ,5 Strip Tubes and Caps,.1 ml (25) PCR Tubes,.2 ml (1) 25 strips of 4 tubes and caps for 1 reactions , 1 thin-walled tubes for 1 reactions , Rotor-Disc OTV Kit Kit for optical temperature verification of Rotor-Gene Q systems; requires Rotor-Disc 72 Rotor and Locking Ring or Rotor-Disc 72 Starter Kit , Rotor-Gene SYR Green For 4 x 25 µl reactions: 2x Master Mix, RNase-Free Water , PCR Kit (4) Rotor-Gene SYR Green RT-PCR Kit (4) For 4 x 25 µl reactions: 2x Master Mix, RT Mix, RNase-Free Water , Rotor-Gene Probe PCR For 4 x 25 µl reactions: 2x Master Mix, RNase-Free Water ,5 Kit (4) Rotor-Gene Probe RT-PCR Kit (4) For 4 x 25 µl reactions: 2x Master Mix, RT Mix, RNase-Free Water , Rotor-Gene Multiplex PCR For 4 x 25 µl reactions: 2x Master Mix, RNase-Free Water ,5 Kit (4) * Rotor-Gene Q 7/215 13
14 Cat. no. QuantiTect Primer ssay (2)* For 2 x 5 µl reactions: 1x QuantiTect Primer ssay (lyophilized) Varies 18, QuantiTect Reverse Transcription Kit (5)* miscript SYR Green PCR Kit (2)* QuantiTect Virus +ROX Vial Kit (2)* For 5 x 2 µl reactions: uffers, Quantiscript Reverse Transcriptase, RT Primer Mix, RNase-Free Water For 2 x 5 µl reactions: 2x Master Mix, Universal Primer, RNase-Free Water For 2 x 5 µl reactions: 5x Master Mix, RT Mix, RNase-Free Water, Nucleic cid Dilution uffer , , , Type-it Fast SNP Probe PCR Kit (8)* EpiTect MethyLight PCR Kit (2)* For 8 x 25 µl reactions: 2x Master Mix, RNase-Free Water, , Q-Solution For 2 x 5 µl reactions: 2x Master Mix, RNase-Free Water ,5 EpiTect HRM PCR Kit (1) For 1 x 25 µl reactions: 2x Master Mix, RNase-Free Water ,5 Type-it HRM PCR Kit (1) For 1 x 25 µl reactions: 2x Master Mix, RNase-Free Water , * miscript PCR System Rotor-Gene Q QIGEN Kit QIGEN Kit Rotor-Gene Q QIGEN Trademarks and Disclaimers QIGEN Handbook User Manual Rotor-Gene Q 14 Rotor-Gene Q 7/215
15 Trademarks: QIGEN, EpiTect, GeneGlobe, Q-ond, Q-Solution, Quantiscript, QuantiTect, Rotor-Gene, Rotor-Disc, Type-it (QIGEN Group); lexa Fluor, SYR, Texas Red (Molecular Probes, Inc.); CL Fluor, Quasar (iosearch Technologies, Inc.); Cy (GE Healthcare); FM, JOE, ROX, VIC (pplera Corporation or its subsidiaries); Lightr, TaqMan (Roche Group). 215 QIGEN, all rights reserved. Rotor-Gene Q 7/215 15
16 Forefront Tower II Tel: Fax: /215
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