試薬消耗品012_p1-40_30th.indd

Transcription

1 PCR

2 Contents PCR...3 PCR TaqMan...6 TaqMan / PCR / PCR...8 PCR RNA...10 Cells-to-CT Kit...12 Cell-to-CT Kit RT-PCR TaqMan Fast...18 TaqMan...19 SYBR Green...22 TaqMan...24 TaqMan TaqMan TaqMan...29 RNAi...32 sirna...33 RNA sirna mirna...36 microrna...37 microrna...38 microrna...39 microrna...40 Non-coding RNA...42 microrna...43 SNP...44 SNP...45 SNP High Resolution Melt HRM TaqMan INDEX...63 INDEX...69

3 PCR PCR TaqMan SYBR Green RNA PureLink RNA Kit TRIzol Reagent TRIzol Plus RNA Purification System TaqMan Cells-to-CT Kit Ambion Single Cell-to-CT Kit MagMax RNAqueous Kit SYBR Green Cells-to-CT Kit Super Script VILO 16 High Capacity RNA-to-cDNA Kit 17 1 RT-PCR TaqMan RNA-to-CT 1-Step Kit TaqMan Fast Virus 1-Step Master Mix Power SYBR RNA-to-CT 1-Step Kit 15 PCR TaqMan Fast Advance Master Mix TaqMan Fast Universal PCR Master Mix TaqMan Universal Master Mix II TaqMan Gene Expression Master Mix Power SYBR Green PCR Master Mix Fast SYBR Green Master Mix SYBR Select Master Mix TaqMan TaqMan Gene Expression Assays TaqMan Array Plates TaqMan Array Gene Signature 96-well Plate TaqMan Gene Signature Arrays TaqMan Custom Arrays RNAi sirna Silencer Select sirna TaqMan sirna Assays Ambion In Vivo sirna Lipofectamie RNAiMAX Invivofectamine microrna mirvana mirna Isolation Kit mirvana PARIS Kit TaqMan mirna ABC Purification Kit microrna TaqMan MicroRNA RT Kit 39 Megaplex Primer Pools 39 PCR TaqMan Universal Master Mix II 19 TaqMan TaqMan MicroRNA Assays TaqMan Array MicroRNA Card TaqMan Pri-miRNA Assays TaqMan Non-coding RNA Assays microrna microrna mimic & inhibitor mirvana mirna mimic & inhibitors 43 RNAiMAX Lipofectamine Invivofectamine SNP TaqMan Genotyping Master Mix 46 PCR TaqMan GTXpress Master Mix 46 TaqMan TaqMan SNP Genotyping Assays 45 1 SNP TaqMan Sample-to-SNP Kit 47 TaqMan TaqMan Mutation Detection Assays 48 TaqMan TaqMan Copy Number Assays 49 HRM PCR MeltDoctor HRM 50 TaqMan TaqMan Protein Expression Core Reagents Kit with Master Mix TaqMan Protein Assay Kit TaqMan Protein Assay Open Kit Protein Thermal Shift Dye Kit 55 Protein Thermal Shift Starter Kit 55 3

4 PCR TaqMan SYBR Green PCR TaqMan 5'- TaqMan TaqMan PCR TaqMan PCR5 - TaqMan PCR 5'- TaqMan TaqMan PCR DNA 5'-3' TaqMan 1.Polymerization TaqMan R Q 5' 3' TaqMan Gene Expression Assays Strand displacement TaqMan 3.Cleavage DNA 5'- 3' TaqMan 5' 4.Polymerization completed PCR 1. TaqMan 4

5 SYBR Green SYBR Green SYBR Green2 DNA SYBR Green I PCR 2 DNA PCRSYBR Green I PCR Tm PCR 2 DNA SYBR Green I Dye DNA SYBR Green AmpliTaq Gold DNA Polymerase 1.Reaction setup SYBR Green I Dye 2 DNA 2.Denaturation DNA SYBR Green I Dye DNA 3.Polymerization PCR 4.Polymerization completed SYBR Green I Dye 2 DNA PCR 2. SYBR Green 1. TaqMan SYBR Green TaqMan SYBR Green TaqMan 2 DNA SYBR Green I 3 PCR 2 TaqMan 2 PCR PCR TaqMan Gene Expression Assays PCR 5

6 TaqMan TaqMan TaqMan microrna mirna Small RNASNP TaqMan SNP TaqMan PCR TaqMan TaqMan Gene Expression Assay / FAM VIC 4 2,700 TaqMan Drug Metabolism Genotyping Assay PharmaADME DME mirna non-coding RNA mirna non-codingrna ncrna mirna pri-mirna small RNA mirna ncrna TaqMan Protein Assay SNP TaqMan SNP 450 TaqMan SNP Genotyping Assay Custom TaqMan SNP Genotyping Assay TaqMan Copy Number Assay Custom Plus TaqMan Copy Number Assays Custom TaqMan Copy Number Assays 2. TaqMan TaqMan Gene Expression 1 TaqMan MGB 24 Assays cdna Custom TaqMan Gene TaqMan Gene Expression Assays 24 Expression Assays TaqMan Array Plates TaqMan Gene Expression Assays 96 5 Fast 25 TaqMan Array Gene Signature 96-Well Plate TaqMan Gene Signature Arrays TaqMan Custom Arrays TaqMan Array Human Endogenous Control Plate Custom Plus TaqMan RNA Assays 96 TaqMan Gene Expression Assays 384 TaqMan TaqMan 32 TaqMan Gene Expression Assays

7 mirna non-coding RNA Megaplex RT Primer Pools for TaqMan MicroRNA Assays mirna cdna Multiplex RT 1 mirna 39 TaqMan MicroRNA Assays TaqMan mirna mirna TaqMan Array MicroRNA Card TaqMan MicroRNA Assays 384 TaqMan 8 TaqMan Non-coding RNA Assays TaqMan Non-coding RNA Assays non-coding 42 TaqMan Pri-miRNA Assays mirnaprimary mirna pri-mirna SNP TaqMan Pre-Designed SNP Genotyping Assays SNP SNP 2 TaqMan PCR 45 TaqMan Drug Metabolism Genotyping Assays TaqMan Copy Number Assays Custom TaqMan Copy Number Assays & Custom Plus TaqMan Copy Number Assays TaqMan Copy Number Reference Assays TaqMan Mutation Detection Assays 221 2,700 Genotyping TaqMan CNV TaqMan CNVs TaqMan Copy Number Assays TaqMan Copy Number Reference Assays PCR 3 4 TaqMan Copy Number Assays CNVs TaqMan Copy Number Reference Assays TaqMan Copy Number Assays Custom TaqMan Copy Number Assays TaqMan Competitive Allele-Specific TaqMan PCR castpcr MGB TaqMan Protein Assay Open Kit / TaqMan Protein Assays Core Reagents Kit with Master Mix TaqMan Protein Assay TaqMan 5 TaqMan Custom Plating Service TaqMan Gene Expression Assays TaqMan MicroRNA Assays / / 96 Standard 96 Fast 384 TaqMan Gene Expression Assays Inventoried, Made-to-Order, and Custom 24 TaqMan MicroRNA Assays Inventoried, Made-to-Order, and Custom 40 TaqMan Non-coding RNA Assays Inventoried, Made-to-Order 42 TaqMan SNP Genotyping Assays Predesigned Custom and DME 45 TaqMan Copy Number Assays Predesigned Custom Plus or Custom

8 TaqMan TaqMan TaqMan Array OpenArray 1 PCR Applied Biosystems Quant Studio 12K Flex ViiA HTPCR TaqMan Array 1 Applied Biosystems Quant Studio 12K Flex OpenArray PCR / PCR PCR StepOne PCR 48 3 Fast StepOnePlus TM StepOnePlus 96 4 Fast PCR VeriFlex TM QuantStudio TM 12K Flex Fast 384 TaqMan Array OpenArray ,000 OptiFlex LED 21 microrna PCR ViiA TM 7 PCR 21 OptiFlex Fast 384 TaqMan Array 4 QuantStudio TM 3D PCR 20,000 NEW 8

9 PCR QuantStudio 12K Flex PCR PCR PCR PCR PCR PCR PCR PCR PCR 3 QuantStudio 12K Flex PCR PCR QuantStudio 12K Flex PCR OpenArray QuantStudio 12K Flex PCR QuantStudio PCR 1 49,000 PCR DNA DNA PCR 3. QuantStudio 12K Flex TaqMan OpenArray PCR BK AcroMetrix BK Viral StandardDNA OpenArray PCR PCR 15,800 / L QuantStudio 12K FlexPCR QuantStudio 12K Flex OpenArray OpenArray 2 OpenArray Accufill DigitalSuite Windows 7 1 QuantStudio Digital PCR Kit, 10-pack QuantStudio Digital ,700 2X TaqMan OpenArray Digital PCR Master Mix 5 ml QuantStudio 12K Flex OpenArray Accessories Kit 10 QuantStudio Digital PCR Kit, 4-pack QuantStudio PCR 4 2X TaqMan OpenArray Digital PCR Master Mix 5 ml ,700 QuantStudio 12K Flex OpenArray Digital PCR Starter Kit OpenArray Digital PCR Plate 2 TaqMan OpenArray Digital PCR Master Mix 1.5 ml Human MaleDNA TaqMan Assay RNase P OpenArray Accessories Kit 222,200 DigitalSuite Software PCR ,000 QuantStudio 12K Flex OpenArray Accessories Kit QuantStudio 12K Flex OpenArray PCR Cases 10 QuantStudio 12K Flex OpenArray Immersion Fluids 10 QuantStudio 12K Flex OpenArray Case Sealing Glues 2 38,900 9

10 RNA RNA PureLink RNA Kit PureLink RNA Mini Kit RNA RNA 1,000µg 100µg DNase Set DNA RNA µg 1, PureLink RNA Mini Kit Competitor s midi kit Competitor s mini kit Tissue mg 4. RNA PureLink RNA Mini Kit RNA PureLink RNA Mini Kit PureLink RNA Mini Kit , A 28, ,000 PureLink RNA Micro Kit ,000 PureLink Homogenizer ,000 PureLink DNase Set ,500 RNA TRIzol Reagent TRIzol Reagent DNA RNA TRIzol LS Reagent RNA TRIzol Reagent 100 L , L , L SP 158,000 TRIzol LS Reagent 100 L , L ,800 RNA TRIzol Plus RNA Purification System TRIzol Plus RNA Purification System Purelink RNA Mini Kit No.1 TRIzol 45RNA TRIzol RNA TRIzol Plus RNA Purification System TRIzol Reagent 100mL PureLink RNA Mini Kit A , RNA TRIzol Reagent PureLink Kits TRIzol Plus RNA Purification System MagMAX Kits TRIzol mg (1mL ) 200mg mg mg

11 RNA MagMax RNA RNA MagMax RNA MagMAX Total Nucleic Acid 100 AM ,000 Isolation Kit MagMAX -96 Blood RNA Isolation Kit 96 AM ,800 MagMAX -96 Total RNA Isolation Kit 96 AM ,500 MagMAX Viral RNA Isolation Kit 50 AM ,000 MagMAX -96 Viral RNA Isolation Kit 96 AM ,900 RNA Single Place Magnetic Stand 1 AM ,400 Magnetic Stand-96 1 AM , well Magnetic-Ring Stand 1 AM ,000 RNA RNAqueous Kit RNAqueous Kit RNA 20 RNA DNA RNARNAqueous -4PCR Kit RNAqueous Kit 50 AM ,400 RNAqueous Midi Kit 15 AM ,500 RNAqueous -4PCR Kit 30 AM ,600 RNAqueous -96 Kit 192 AM ,000 RNAqueous -96 Automated Kit 4 96 AM ,000 RNAqueous -Micro Kit 50 AM ,400 RNA RNAlater Solution RNAlater Solution RNA RNAlater Solution RNAlater Solution 100mL AM , mL AM , mL AM , mL AM , mL AM ,500 RNA RNAlater -ICE RNAlater -ICE RNA RNAlater -ICE -20 RNA RNAlater -ICE 25mL AM ,700 DNA TURBO DNA-free Kit TURBO DNA-free Kit DNA TURBO DNase TURBO DNase DNase I DNA DNase I 6 RT-PCR false positive DNA TURBO DNA-free Kit 50 AM ,600 RNA THE RNA Storage Solution 10 1mL AM7000 7,400 50mL AM7001 7,400 TE Buffer ph mL AM9860 7,400 50mL AM9861 7, mm EDTA 50mL AM9912 7,400 11

12 Cells-to-CT Kit RNA Cells-to-CT Kit Cells-to-CT Kit RNA PCR RNA Cells-to-CT Kit sirnamicrorna mirna gdna PCR DNase ,000 RNA TaqMan Gene Expression Cells-to-CT Kit TaqMan Fast Cells-to-CT Kit Power SYBR Green Cells-to-CT Kit Fast SYBR Green Cells-to-CT Kit TaqMan PreAmp Cells-to-CT Kit mirna TaqMan MicroRNA Cells-to-CT Kit 5. RNA 6. Cells-to-CT Kit PCR Cells-to-CT CTs HeLa RNA Cells-to-CT Kit 128 TaqMan Gene Expression Assays Applied Biosystems 7900HT PCR Cells-to-CT Kit Ct RNACt Purified RNA C T s 12

13 TaqMan TaqMan Gene Expression Cells-to-CT Kit TaqMan Gene Expression Cells-to-CT Kit RNA RNA DNA cdna PCR TaqMan Gene Expression Master Mix TaqMan Gene Expression Cells-to-CT Kit , AM , AM ,000 * TaqMan Cells-to-CT Control Kit/Stop Solution TaqMan Cells-to-CT Control Kit TaqMan Gene Expression Cells-to-CT Kit PCR TaqMan Cells-to-CT Control Kit ,000 Cells-to-CT Stop Solution 1 ml ,000 SYBR Green Power SYBR Green / Fast SYBR Green Cells-to-CT Kit SYBR Green Cells-to-CT Kits RNA SYBR Green DNA PCR Power SYBR Green Master Mix Fast SYBR Green Master Mix Power SYBR Green Cells-to-CT Kit Power SYBR Green Cells-to-CT Kit , , ,000 * Fast SYBR Green Cells-to-CT Kit Fast SYBR Green Cells-to-CT Kit , ,000 * SYBR Green Cells-to-CT Control Kit SYBR Green Cells-to-CT Control Kit ,200 TaqMan Fast Cells-to-CT Kit TaqMan Fast Cells-to-CT Kit RNA Fast PCR PCR TaqMan Fast Universal PCR Master Mix TaqMan Fast Cells-to-CT Kit ,000 * mirna TaqMan MicroRNA Cells-to-CT Kit TaqMan MicroRNA Cells-to-CT Kit RNA mirna mirna TaqMan MicroRNA Cells-to-CT Kit ,000 * TaqMan PreAmp Cells-to-CT Kit TaqMan PreAmp Cells-to-CT Kit TaqMan Gene Expression Cells-to-CT Kit RNA TaqMan Gene Expression Master Mix cdna TaqMan PreAmp Master Mix TaqMan PreAmp Cells-to-CT Kit ,000 * 13

14 Cell-to-CT Kit Ambion Single Cell-to-CT Kit Ambion Single Cell-to-CT Kit 1 10 RNA 5 Pre-Amplification PCR RNA Ambion Single Cell Lysis Kit RNA Ambion Single Cell Lysis Kit RNA RNA PCR Single Cell-to-CT Kit Cell Equivalent Single Cells 100 Cells 7. Single Cell-to-CT Kit CT CT T 1 Ambion Single Cell-to-CT Kit , ,000 Ambion Single Cell Lysis Kit ,000 * DNA 10 L Single Cell Solution L Stop Solution 2 Cells-to-CpG Bisulfite Conversion Kit Cells-to-CpG Bisulfite Conversion Kit DNA FFPE DNA Single Cell Lysis Buffer PBS / 5 3. PBS RT cdna 14 Pre-Amplification B2M GAPDH HPRT1 TGFB1 5 Ct Cells-to-CpG Bisulfite Conversion Kit , ,000 Cells-to-CpG Bisulfite Conversion & ,500 Quantitation Control Kit Cells-to-CpG Methylated and Unmethylated gdna Control Kit 50µL ,000 14

15 1 RT-PCR TaqMan RNA-to-CT 1-Step Kit TaqMan RNA-to-CT 1-Step Kit 1 TaqMan 1 1 TaqMan RNA TaqMan Fast Virus 1-Step Master Mix 21 1 AT GC TaqMan Gene Expression Assays 24 TaqMan RNA-to-CT 1-Step Kit, 1mL ,000 TaqMan RNA-to-CT 1-Step Kit, 5mL ,000 TaqMan RNA-to-CT 1-Step Kit, 2, ,000 5mL 10 TaqMan RNA-to-CT 1-Step Kit TaqMan RNA-to-CT 1-Step Kit TaqMan One-Step RT-PCR Mater Mix Reagents Kit RT 1 RT-PCR TaqMan One-Step RT-PCR Master ,000 Mix Reagents Kit 10-Pack, TaqMan One-Step RT-PCR 2, ,000 Master Mix Reagents Kit TaqMan One-Step RT-PCR Master Mix Reagents Kit Power SYBR Green RNA-to-CT 1-Step Kit Power SYBR Green RNA-to-CT 1-Step Kit PCR 1 SYBR Green Rn Cycle Power SYBR Green RNA-to-CT ,000 1-Step Kit, 1mL Power SYBR Green RNA-to-CT ,000 1-Step Kit, 5mL Power SYBR Green RNA-to-CT Step Kit Power SYBR Green RNA-to-CT 1-Step Kit Derivative Reporter (-Rn) Temperature (ºC) 10. Power SYBR Green RNA-to-CT 1-Step Kit GAPDH RNA BOSC23 Applied Biosystems StepOnePlus PCR 3 TaqMan EZ RT-PCR Core Reagents RT 1 RT-PCR TaqMan EZ RT-PCR Core Reagents 200 N , Pack, TaqMan EZ RT-PCR Core 2, ,120,000 Reagents TaqMan EZ RT-PCR Kit

16 PCR SuperScript VILO SuperScript VILO Variable Input Linear Output SuperScript RT PCR mrna cdna RNA SuperScript VILO Master Mix 1 VILO SuperScript VILO PCR cdna PCR 1pg 2.5 g RNA cdna RNA cpm , , ,965 0 S VILO A 11. cdna 32 P-dCTP cdna S A SuperScript VILO (VILO) A 9 VILO cdna 13. cdna PCR TaqMan TfR Target SuperScript VILO cdna Synthesis Kit SuperScript VILO cdna Synthesis Kit , ,400 SuperScript VILO Master Mix SuperScript VILO Master Mix , , ,900 RNA cdna RNA cdna 12. RNA cdna VILO RNA cdna 4. High Capacity cdna High Capacity RNA-tocDNA Kit cdna Synthesis Kit Master Mix SuperScript VILO SuperScript VILO Reverse Transcriptase Kit MultiScribe MultiScribe Superscript III Superscript III 37 C 37 C 42 C 42 C (Total RNA) 20ng-2 g 20ng-2 g 1pg-2.5 g 1pg-2.5 g RNase H

17 High Capacity RNA-to-cDNA Kit High Capacity RNA-to-cDNA Kit PCR cdna RNA PCRHigh Capacity RNA-to-cDNA Kit RNA cdna µg RNA 2 RT-PCR * High Capacity RNA-to-cDNA Kit ,800 High Capacity RNA-to-cDNA Kit High Capacity RNA-to-cDNA Kit *20µL High Capacity cdna Reverse Transcription Kit High Capacity cdna Reverse ,600 Transcription Kit 1, ,000 High Capacity cdna Reverse Transcription Kit, with RNase Inhibitor ,000 1, ,000 High Capacity cdna Reverse Transcription Kit RNase Inhibitor, 20units/µL 100 N ,600 *20µL TaqMan Reverse Transcriation Reagents TaqMan Reverse Transcription 200 N ,400 Reagents 10-Pack, TaqMan Reverse Transcription Reagents 2, ,000 / / MultiScribe Reverse Transcriptase 5,000units ,100 RNase Inhibitor, 20units/µL 2,000units N ,600 Random Hexamers, 5nmoles 0.1mL N ,000 Oligo d(t) 16 Primers, 5nmoles 0.1mL N ,000 GeneAmp dntp Blend, 10mM 1mL N ,000 GeneAmp dntp Blend, 12.5mM with dutp 100 N ,200 AmpErase Uracil N-glycosylase 100units N ,000 UNG 20% Glycerol Solution, Molecular 100mL ,000 Biology Grade TaqMan Control Total RNA Human 100µL ,000 50ng/µL TaqMan Control Genomic DNA Human 10ng/µL, 2 100µL ,000 17

18 TaqMan Fast Fast PCR 4045 Fast Fast 40 TaqMan Fast Advanced Master Mix FastFast PCR Fast Fast PCR Rn Cycle Number TaqMan Fast Advanced Master Mix 40 TaqMan Fast Universal PCR Master Mix TaqMan Fast Universal PCR Master Mix TaqMan Fast PCR Fast PCR Fast PCR Fast AmpliTaq Fast DNA Polymerase 72 PCR TaqMan Assays TaqMan Array Fast Fast Fast TaqMan Fast Universal PCR Master Mix ,000 2X, No AmpErase UNG 2-Pack, TaqMan Fast Universal PCR ,000 Master Mix 2X, No AmpErase UNG 5-Pack, TaqMan Fast Universal PCR 1, ,000 Master Mix 2X, No AmpErase UNG 10-Pack, TaqMan Fast Universal PCR Master Mix 2X, No AmpErase UNG 2, ,000 TaqMan Fast Universal PCR Master Mix 5, ,000 50mL TaqMan Fast Universal PCR Master Mix *20µL TaqMan Fast Advanced Master Mix, ,000 1mL TaqMan Fast Advanced Master Mix, ,000 5mL TaqMan Fast Advanced Master Mix, 1, , mL TaqMan Fast Advanced Master Mix, 2, , mL TaqMan Fast Advanced Master Mix, 5, , mL TaqMan Fast Advanced Master Mix, 5, ,000 50mL *20µL 18

19 TaqMan 1 TaqMan Universal Master Mix II TaqMan Universal Master Mix II microrna mirna SNP CNV TaqMan Universal Master Mix II 1 RNase P TaqMan Universal Master Mix II PCR PCR 24 SNP mirna TaqMan Assays TaqMan Assays PCR UNG PCR TaqMan Universal Master Mix II, with UNG TaqMan Universal Master Mix II, ,000 with UNG 1mL TaqMan Universal Master Mix II, ,000 with UNG 5mL TaqMan Universal Master Mix II, ,000 with UNG 2 5mL TaqMan Universal Master Mix II, 1, ,000 with UNG 5 5mL TaqMan Universal Master Mix II, 2, ,000 with UNG 10 5mL TaqMan Universal Master Mix II, with UNG 50mL 2, ,000 TaqMan Universal Master Mix II, no UNG TaqMan Universal Master Mix II, ,000 no UNG 1mL TaqMan Universal Master Mix II, ,000 no UNG 5mL TaqMan Universal Master Mix II, ,000 no UNG 2 5mL TaqMan Universal Master Mix II, 1, ,000 no UNG 5 5mL TaqMan Universal Master Mix II, 2, ,000 no UNG 10 5mL TaqMan Universal Master Mix II, no UNG 50mL 2, ,000 *50µL PCR24 PCR TaqMan Universal Master Mix II PCR PCR

20 TaqMan TaqMan Gene Expression Master Mix TaqMan Gene Expression Master Mix PCR GC AT PCR TaqMan Universal PCR Master Mix TaqMan Universal PCR Master Mix TaqMan 5' PCR AmpliTaq Gold DNA Polymerase 72 TaqMan Gene Expression Master Mix, ,000 1mL TaqMan Gene Expression Master Mix, ,000 5mL TaqMan Gene Expression Master Mix, , mL TaqMan Gene Expression Master Mix, 1, , mL TaqMan Gene Expression Master Mix, 2, , mL TaqMan Gene Expression Master Mix, 2, ,000 50mL TaqMan Gene Expression Master Mix TaqMan Gene Expression Master Mix *50µL RNA PCRWater Nuclease free Water Nuclease-free Water 500mL AM ,400 1,000mL AM , mL AM , mL AM9938 5, mL AM ,500 TaqMan Universal PCR Master Mix ,000 2-Pack, TaqMan Universal PCR Master Mix , Pack, TaqMan Universal PCR Master Mix 1, , Pack, TaqMan Universal PCR Master Mix 2, , TaqMan Universal PCR Master Mix, 10-Pack 2, ,000 5mL 10 TaqMan Universal PCR Master Mix, 50 ml 2, ,000 TaqMan Universal PCR Master Mix *50µL TaqMan Universal PCR Master Mix, No AmpErase UNG AmpErase UNG TaqMan 5' PCR AmpliTaq Gold DNA Polymerase TaqMan Universal PCR Master Mix, ,000 No AmpErase UNG TaqMan Universal PCR Master Mix, ,000 No AmpErase UNG, 2-Pack TaqMan Universal PCR Master Mix, 1, ,000 No AmpErase UNG, 5-Pack TaqMan Universal PCR Master Mix, 2, ,000 No AmpErase UNG, 10-Pack TaqMan Universal PCR Master Mix, 2, ,000 No AmpErase UNG, 50 ml TaqMan Universal PCR Master Mix *50µL 20

21 PCR TaqMan Fast Virus 1-Step Master Mix PCR RNA DNA 1 4 RNA / DNA PCR Fast RT-PCR PCR PCR TaqMan Fast Virus 1-Step Master Mix Fast PCR TaqMan Fast Virus 1-Step Master Mix PCR 4 Fast TaqMan EZ RT-PCR Core Reagents TaqMan RNA-to-C T 1-Step Kit TaqMan Fast Virus 1-Step Master Mix TaqMan PreAmp Master Mix TaqMan PreAmp Master Mix cdna PrePCR PrePCR TaqMan Gene Expression Assays 100 1ngcDNA 200 PCR TaqMan PreAmp Master Mix ,000 TaqMan PreAmp Master Mix Kit ,000 PreAmp Master Mix ( ) TaqMan Gene Expression Master Mix, 5mL ( ) TaqMan PCR Core Reagents Kit TaqMan PCR Core Reagents Kit TaqMan PCR TaqMan PCR Core Reagents Kit with 200 N ,000 AmpliTaq Gold 10-Pack, TaqMan PCR Core Reagent Kit 2, ,000 TaqMan PCR Reagent Kit *50µL TaqMan Gold with Buffer A Pack 1000 Reaction TaqMan Gold with Buffer A Pack 1, ,000 *50µL TaqMan Reagents Starter Kit TaqMan Reagents Starter Kit 1Kit ,000 TaqMan Universal Master Mix, No UNG 1.25mL TaqMan Gene Expression Assay, Eukaryotic 18S rrna, 20X 0.3mL Human Raji cdna, 25ng/µL 0.05mL Fast TaqMan Fast Virus 1-Step Master Mix 1 1 TaqMan Fast Virus 1-Step Master Mix TaqMan Fast Virus 1-Step Master Mix, ,600 1mL TaqMan Fast Virus 1-Step Master Mix, 1, , mL TaqMan Fast Virus 1-Step Master Mix, 10mL 2, ,000 *20µL 21

22 SYBR Green Power SYBR Green PCR Master Mix Power SYBR Green PCR Master Mix 2 DNA cdna RNA PCR SYBR Green PCR 2 Power SYBR Green PCR Master Mix, ,000 1mL Power SYBR Green PCR Master Mix, ,000 5mL 2-Pack, Power SYBR Green PCR Master ,000 Mix, 2 5mL 5-Pack, Power SYBR Green PCR Master 1, ,000 Mix, 5 5mL 10-Pack, Power SYBR Green PCR Master 2, ,000 Mix, 10 5mL Power SYBR Green PCR Master Mix, 2, ,000 50mL Power SYBR Green PCR Master Mix *50µL Fast SYBR Green Master Mix Fast SYBR Green Master Mix Fast PCR Fast PCR PCR Fast SYBR Green Master Mix, 1mL ,000 Fast SYBR Green Master Mix, 5mL ,000 2-Pack, Fast SYBR Green Master Mix, 1, , mL 5-Pack, Fast SYBR Green Master Mix, 2, , mL 10-Pack, Fast SYBR Green Master Mix, 5, , mL Fast SYBR Green Master Mix, 50mL 5, ,000 Fast SYBR Green Master Mix Fast SYBR Green Master Mix *20µL 1.0e e+001 Delta Rn vs Cycle 50 ng 5 ng 500 pg 50 pg 5 pg 18. Fast SYBR Master Mix CT Delta Rn 1.0e e e Cycle Number Standard Curve pg Slope = pg 28 R 2 = pg ng ng Log Concentration 17. Human gdna -actin SYBR Green PCR Master Mix SYBR Green PCR Master Mix SYBR Green PCR 2 SYBR Green PCR Master Mix, 1mL ,000 SYBR Green PCR Master Mix, 5mL ,000 SYBR Green PCR Master Mix, 50mL 2, , Pack, SYBR Green PCR Master Mix, 2, , mL SYBR Green PCR Master Mix *50µL SYBR Green PCR Core Reagents SYBR Green PCR Core Reagents , Pack, SYBR Green PCR Core 2, ,000 Reagents SYBR Green PCR Core Reagents *50µL 22

23 A SYBR Select Master Mix SYBR Select Master Mix PCR Rn Cycle DNA PCR B Rn 1E01 1E00 SYBR GreenER 0.1 Cycle PCR 72 PCR 72 PCR UDG 19. cdna A cdna PGK1 PCR 100 ng cdna B 1 ng 2 ng cdna ViiA 7 PCR A PCR Applied Biosystems PCR SYBR Select Master Mix, mini Pack ,700 1mL SYBR Select Master Mix, 1 Pack ,800 5mL SYBR Select Master Mix, 2 Pack 1, , mL SYBR Select Master Mix, 5 Pack 2, , mL SYBR Select Master Mix, 10 Pack 5, , mL SYBR Select Master Mix, Bulk Pack 50mL 5, ,000 *20µL B Distribution qpcr C t A DNA DNA PCR 50 DNA B RNase PCt Ct SYBR Select Master Mix ViiA 7 PCR 23

24 TaqMan TaqMan Gene Expression Assays TaqMan Gene Expression Assays PCR & 130 Exon RNAi FAM TaqMan Gene Expression Assays, Inventoried ,800 TaqMan Gene Expression Assays, Small, Made-to-Order TaqMan Gene Expression Assays, Medium, Made-to-Order TaqMan Gene Expression Assays, Large, Made-to-Order VIC , ,800 2, ,500 TaqMan Gene Expression Assays, ,000 Small_VIC, Made-to-Order TaqMan Gene Expression Assays, ,800 Medium_VIC, Made-to-Order TaqMan Gene Expression Assays, Large_VIC, Made-to-Order 2, ,500 (Primer Limited)TaqMan Assays VIC TaqMan Gene Expression Assays, ,000 Small_VIC, PL Made-to-Order TaqMan Gene Expression Assays, ,800 Medium_VIC, PL Made-to-Order TaqMan Gene Expression Assays, Large_VIC, PL Made-to-Order 2, ,500 *20µL Inventoried Made-to-Order TaqMan Gene Expression Assays Species Number of Assays H. sapiens (Human) 212,207 M. musculus (Mouse) 185,566 R. norvegicus (Rat) 151,955 O. sativa (Rice) 99,822 A. thaliana (Arabidopsis) 97,879 C. elegans (Nematode) 92,687 B. taurus (Cow) 67,104 M. mulatta (Rhesus Monkey) 66,480 D. rerio (Zebrafish) 63,712 X. tropicalis (Frog) 56,764 C. familiaris (Dog) 55,202 G. gallus (Chicken) 48,432 Custom Plus TaqMan RNA Assays Custom Plus TaqMan RNA Assays TaqMan Gene Experssion Assays Custom Plus TaqMan RNA Assays Custom Plus TaqMan RNA Assay, Small , Custom Plus TaqMan RNA Assay, Medium ,900 Custom Plus TaqMan RNA Assay, Large 60 Species Number of Assays D. melanogaster (Fruit Fly) 41,607 Z. mays (Sweet Corn) 38,446 S. scrofa (Pig) 9,868 S. pombe (Fission Yeast) 6,538 O. cuniculus (Rabbit) 5,924 S. cerevisiae (Baker's Yeast) 5,525 G. max (Soybean) 3,456 E. caballus (Horse) 2,970 C. porcellus (Guinea Pig) 2,037 V. vinifera (Grape) 965 T. aestivum (Wheat) 760 Total Number of Assays 1,315,906 Custom TaqMan Gene Expression Assays Custom TaqMan Gene Expression Assays TaqMan Gene Expression Assays TaqMan Gene Expression Assays TaqMan Custom TaqMan Gene 50µL ,000 Expression Assays, S-scale 20µL 360 Custom TaqMan Gene 50µL ,900 Expression Assays, M-scale 20µL 750 Custom TaqMan Gene Expression Assays, L-scale 50µL 1,160 20µL 2, ,600 2, ,600 24

25 TaqMan 96 TaqMan Array Plate 96 - Fast TaqMan Array Plate TaqMan Gene Expression Assays Fast 10 TaqMan Gene Expression Assays 6 96 PCR TaqMan Standard : 7900HT / ViiA 7 / 7500 / 7300 / QuantStudio 96 Fast : 7900HT / ViiA 7 /7500Fast /StepOnePlus / QuantStudio Fast96 60 TaqMan Array Plate ** * TaqMan Array Plates, Format 8 (Standard) TaqMan Array Plates, Format 8 (Fast) TaqMan Array Plates, Format 16 (Standard) TaqMan Array Plates, Format 16 (Fast) TaqMan Array Plates, Format 16 + Endogenous Controls (Standard) TaqMan Array Plates, Format 16 + Endogenous Controls (Fast) TaqMan Array Plates, Format 32 (Standard) TaqMan Array Plates, Format 32 (Fast) TaqMan Array Plates, Format 32 + Endogenous Controls (Standard) TaqMan Array Plates, Format 32 + Endogenous Controls (Fast) TaqMan Array Plates, Format 48 (Standard) TaqMan Array Plates, Format 48 (Fast) TaqMan Array Plates, Format 48 + Endogenous Controls (Standard) TaqMan Array Plates, Format 48 + Endogenous Controls (Fast) TaqMan Array Plates, Format 96 (Standard) TaqMan Array Plates, Format 96 (Fast) TaqMan Array Plates, Format 96 + Endogenous Controls (Standard) TaqMan Array Plates, Format 96 + Endogenous Controls (Fast) ,300 26,300 22, ,300 26,300 22, ,400 30,300 26, ,400 30,300 26, ,400 30,300 26, ,400 30,300 26, ,500 39,400 34, ,500 39,400 34, ,500 39,400 34, ,500 39,400 34, ,500 45,500 39, ,500 45,500 39, ,500 45,500 39, ,500 45,500 39, ,600 50,500 45, ,600 50,500 45, ,600 50,500 45, ,600 50,500 45,500 * ** Candidate Endogenous Control GAPDH HPRT1 GUSB 3 25

26 TaqMan 96 TaqMan Array Gene Signature 96-Well Plate TaqMan Array Gene Signature 96-Well Plate TaqMan Gene Expression Assays Standard Fast Human Alzheimer's Disease Human Angiogenesis Human Apoptosis Human Immune Response Human Inflammation Human Phosphodiesterase Human Protein Kinase Pathways Human Stem Cell Pluripotency Mouse Alzheimer's Disease Mouse Immune Response Mouse Stem Cell Pluripotency Rat Inflammation Rat Phosphodiesterase Human Androgens Human Chemokines Human CYP450 & other Oxygenases Human Diabetes Human Estrogens Human Heat Shock Proteins Human Hedgehog Pathway Human Hypoxia Human LDL Pathways Human Ligand Gated Ion Channels Human MAPK Pathways Human Neurotransmitters Human NFkB Pathway Human Osteogenesis Human TGF Pathway Human Tumor Metastasis Human Voltage-Gated Ion Channels Human WNT Pathway Human Induced Intracellular Signaling Human Activation of camp-dependent PKA Human HOX Genes Human Antigen Processing and Presentation by MHCS Human Apoptosis via Death Receptors Human Apoptotic Pathways Triggered By HIV Mouse ABC Transporters Human Nuclear Receptors Human Cell Surface Markers Human BMP Pathway ,400 Standard Fast Human BRCA1 Pathway Human Breast Cancer Regulations by Stathmin Human c-myc and Apoptosis Human camp Pathway Human Cardiomyocyte Differentiation by BMP Receptors Human Cell Cycle Control of Chromosomal Replication Human Cellular Apoptosis Pathway Human Drug Transporters Human Antioxidant Mechanisms Human Complement Pathway Human Colorectal Cancer Metastasis Human CREB Pathway Human Cyclins & Cell Cycle Regulation Human Cytokine Network Human Developmental Phases of Colorectal Cancer Human DNA Damage Induced Sigma Signaling Human DNA Methylation & Transcriptional Repression Human DNA Repair Mechanisms Human EGF Pathway Human Signal Transduction Pathways Human enos Signaling Human Erythropoietin Pathway Human Extracellular Matrix & Adhesion Molecules Human Factors for Cardiogenesis Human FAS Signaling Human FGF Pathway Human GPCR Pathway Human Guidance & Growth Cone Motility Human Transcription Factors (Non-Hox) Human Huntington Disease Pathway Human IGF1R Signaling Human IL-1 Pathway Human IL-10 Pathway Human IL-2 Active 8 & Quiescent T-Cell Human IL-2 Pathway Human AHR Pathway Human IL-3 Pathway

27 Standard Fast Human IL-4 Pathway Human IL-6 Pathway Human IL-9 Pathway Human ILK Signaling Human inos Signaling Human Insulin Receptor Pathway Human Interferon Pathway Human Intracellular Calcium Signaling Human JAK-STAT Pathway Human Growth Factors Human Hematopoiesis Human Melatonin Signaling Human Prion Pathway Human Molecular Mechanisms of Cancer Human NGF Pathway Human nnos Signaling at Neuronal Synapses Human nnos Signaling in Skeletal Muscle Human Notch Signaling Human ABC Transporters Human P53 Mediated Apoptosis Human P53 Signaling Human CMV & MAPK Pathways Human Patho Rheumatoid Arthritis Human PDGF Pathway Human PI3K Signaling Human PI3K Signaling in B-Lymphocyte Human PKA Signaling Human Cholera Infection Human RAS Pathway Human Pancreatic Adenocarcinoma Human Phagocytosis of Microbes Human RHO Family GTPases Human RHOA Pathway Human Pathogenesis of ALS Human Signaling in Gap Junctions Human NFAT & Cardiac Hypertrophy Human SMAD Signaling Network Human Sperm Motility Human T-Cell Receptor and CD3 Complex Human Telomere Extension by Telomerase Human Thrombin Signaling Human TNF Superfamily Pathway ,400 Standard Fast Human TNFR1 Pathway Human TNFR2 Pathway Human Toll Comparative Pathway Human Toll-Like Receptors Pathway Human Transcription of mrna Human Transcription of rrna Human Transcription of trna Human Transcriptional Regulatory Network in Embryonic Stem Cell Human Ubiquitin-Proteasome Dependent Proteolysis Human VEGF Pathway Human Maturity-Onset Diabetes of Young (MODY) Mouse Lipid Regulated Genes Rat Endogenous Control Mouse Endogenous Control Human Endogenous Control

28 TaqMan 384 TaqMan Gene Signature Arrays TaqMan Gene Signature Arrays TaqMan Human Human Immune Array ,000 Human Endogenous Control Array ,900 Human Protein Kinase Array ,000 Human GPCR Array ,200 Human ABC Transporter Array ,000 Human Apoptosis Array ,000 Human Phosphodiesterase Array ,000 Human Inflammation Array ,000 Human Angiogenesis Array ,000 Human Alzheimer's Array ,000 Human Nuclear Receptor Array ,000 Human Stem Cell Pluripotency Array ,000 TaqMan Array Human MicroRNA A Cards v ,000 TaqMan Array Human MicroRNA B Cards v ,000 TaqMan Arrays Human MicroRNA A+B Cards Set v ,600 TaqMan Arrays Human MicroRNA B Cards v ,000 TaqMan Arrays Human MicroRNA A+B Cards v ,600 Mouse Mouse Immune Array ,000 Mouse Endogenus Control Array ,900 Mouse GPCR Array ,200 Mouse Alzheimer's Array ,000 Mouse Stem Cell Pluripotency Array ,000 TaqMan Array Rodent MicroRNA A Cards v ,000 TaqMan Array Rodent MicroRNA B Cards v ,000 TaqMan Array Rodent MicroRNA A+B Cards Set v ,600 Rat Rat Endogenous Control Array ,900 Rat Phosphodiesterase Array ,000 Rat Inflammation Array ,000 Rat GPCR Array ,200 TaqMan Array Rodent MicroRNA A Cards v ,000 TaqMan Array Rodent MicroRNA B Cards v ,000 TaqMan Array Rodent MicroRNA A+B Cards Set v ,600 TaqMan Custom Arrays Micro Fluidic Card TaqMan Custom Arrays 7900HT Fast ViiA 7 QuantStudio PCR TaqMan Custom Arrays 7900HT ViiA 7 QuantStudio TaqMan Array Micro Fluidic Card 2µL 384 TaqMan Gene Signature Arrays TaqMan Array, Format ,700 56,600 45,500 40,400 TaqMan Array, Format ,700 58,600 46,500 41,400 TaqMan Array, Format ,700 58,600 46,500 41,400 TaqMan Array, Format ,800 62,600 48,500 43,400 TaqMan Array, Format ,800 62,600 48,500 43,400 TaqMan Array, Format ,800 64,600 50,500 44,400 TaqMan Array, Format 96a ,800 65,700 56,600 46,500 TaqMan Array, Format 96b ,800 65,700 56,600 46,500 TaqMan Array, Format ,000 72,700 62,600 50,500 TaqMan Array, Format ,300 84,800 66,700 54,500 *TaqMan Gene Expression Assays, Inventoried 24 TaqMan Gene Expression Assays, Made-to-Order

29 TaqMan TaqMan Array Endogenous Control Plate TaqMan Array Endogenous Control Plate TaqMan TaqMan Array Human Endogenous ,000 Controls, 96-well Plate TaqMan Array Human Endogenous ,000 Controls, Fast 96-Well Plate TaqMan Array Mouse Endogenous ,000 Controls, 96-well Plate TaqMan Array Mouse Endogenous ,000 Controls, Fast 96-Well Plate TaqMan Array Rat Endogenous Controls, , well Plate TaqMan Array Rat Endogenous Controls, Fast 96-Well Plate , TaqMan Array Human Endogenous Control Plate Assay ID Gene Symbol TaqMan Human Endogenous Control Array TaqMan Human Endogenous Control Array TaqMan Gene Signature Arrays Gene Expression Assays TaqMan Human Endogenous Control Array Applied Biosystems 7900HT ViiA 7 QuantStudio TaqMan Array TaqMan Human Endogenous Control Array ,900 29

30 TaqMan TaqMan Gene Expression Assays (Endogenous Control) TaqMan Gene Expression Assays TaqMan Endogenous Controls TaqMan Endogenous Controls VIC FAM TaqMan Gene Expression 50µL ,800 Assays, Inventoried 20µL 250 TaqMan Endogenous Control 1,000 ** 99,000 VIC /MGB * 50µL ** Human Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC /MGB FAM /MGB 18S X Eukaryotic 18S rrna Hs _s E E ACTB NM_ actin, beta Hs _m E E GAPDH NM_ glyceraldehyde-3-phosphate dehydrogenase Hs _m E E RPLP0 NM_ ribosomal protein, large, P0 Hs _m E PPIA NM_ peptidylprolyl isomerase A (cyclophilin A) Hs _m E PGK1 NM_ phosphoglycerate kinase 1 Hs _m E B2M NM_ beta-2-microglobulin Hs _m E GUSB NM_ glucuronidase, beta Hs _m E HPRT1 NM_ hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome) Hs _m E TBP M TATA box binding protein Hs _m E TFRC NM_ transferrin receptor (p90, CD71) Hs _m E IPO8 NM_ importin 8 Hs _m1 YWHAZ NM_ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Hs _m1 UBC NM_ ubiquitin C Hs _m1 POLR2A NM_ polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Hs _m1 HMBS NM_ hydroxymethylbilane synthase Hs _m1 * VIC TaqMan Fast Universal Master Mix /

31 Mouse Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC /MGB FAM /MGB 18S X Eukaryotic 18S rrna Hs _s E E ACTB NM_ actin, beta, cytoplasmic Mm _s E E Gapdh NM_ glyceraldehyde-3-phosphate dehydrogenase Mm _g E E Rplp2 (AK ) ribosomal protein, large P2 Mm _s1 Ppia NM_ peptidylprolyl isomerase A Mm _g1 Pgk1 NM_ phosphoglycerate kinase 1 Mm _m1 B2m NM_ beta-2 microglobulin Mm _m1 Gusb NM_ glucuronidase, beta Mm _m1 Hprt1 NM_ hypoxanthine guanine phosphoribosyl transferase 1 Mm _m1 Tbp NM_ TATA box binding protein Mm _m1 Tfrc NM_ transferrin receptor Mm _m1 Ipo8 NM_ importin 8 Mm _m1 Ywhaz NM_ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Mm _g1 Ubc ubiquitin C Mm _m1 Polr2a NM_ polymerase (RNA) II (DNA directed) polypeptide A Mm _m1 Hmbs NM_ hydroxymethylbilane synthase Mm _g1 Rat Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC /MGB FAM /MGB 18S X Eukaryotic 18S rrna Hs _s E E Actb NM_ actin, beta Rn _m E E Gapdh NM_ glyceraldehyde-3-phosphate dehydrogenase Rn _s E Rplp2 NM_ ribosomal protein, large P2 Rn _g1 Ppia NM_ peptidylprolyl isomerase A Rn _m1 Pgk1 NM_ phosphoglycerate kinase 1 Rn _g1 B2m NM_ beta-2 microglobulin Rn _m1 Gusb NM_ glucuronidase, beta Rn _m1 Hprt NM_ hypoxanthine guanine phosphoribosyl transferase Rn _m1 Tbp NM_ TATA box binding protein Rn _m1 Tfrc M transferrin receptor Rn _m1 Hmbs NM_ hydroxymethylbilane synthase Rn _m1 Ywhaz NM_ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Rn _m1 Ubc NM_ ubiquitin C Rn _g1 Ppib NM_ peptidylprolyl isomerase B Rn _m1 Arbp NM_ acidic ribosomal phosphoprotein P0 Rn _g1 * VIC TaqMan Fast Universal Master Mix /

32 RNAi RNAi RNA RNAi RNA2 RNA dsrna dsrna mrna RNAi 1. sirna RNAi 2siRNA sirna sirna33 35 RNAi RNAi RNA dsrna RNAi sirna DicerdsRNA sirna small interfering RNA sirna RISC RNA-induced silencing complexes sirna RISC mrna RISC mrna 30 dsrna sirna Dicer sirna shrna short hairpin RNADNA RNAi 2. sirna sirna sirna sirna sirna sirna36 3. sirna mrna sirna PCR TaqMan mrna TaqMan TaqMan RNAi 32

33 sirna Silencer Select sirna Silencer Select sirna LNA sirna sirna 1/10 1/100 5nM LNA 22. Silencer Select sirna sirna 10siRNA nM sirna HeLa sirnatriplicate 48 mrna sirna mrna % sirna 5nM Silencer Select Pre-designed sirna Silencer Select Pre-designed sirna 2 70% RNA Silencer Select Validated sirna Silencer Select Validated sirna 80% mrna Silencer Select Pre-designed sirna % RNA 7. Silencer Select sirna Silencer Select Pre-designed Silencer Select Validated Custom Design Select sirna Custom Select sirna Silencer Select Negative Control #1 sirna Silencer Select Negative Control #2 sirna Silencer Select GAPDH Positive Control sirna Silencer Select Pre-designed sirna 98 Silencer Select Validated sirna RNA 80 RNA Silencer Select sirna sirna Select sirna 21 sirna Silencer Select sirna Silencer Select sirna sirna 2 Silencer Select GAPDH sirna GAPDH GAPDH sirna 23. / RNAi 33

34 sirna Silencer Select Pre-designed sirna Silencer Select Pre-designed sirna, 1nmol ,000 inventoried, standard purity 5nmol ,000 20nmol ,000 40nmol ,000 Silencer Select Pre-designed sirna, non-inventoried, standard purity Silencer Select Pre-designed sirna, HPLC purified * 1nmol 4 Silencer Select Validated sirna 5nmol ,000 20nmol ,000 40nmol ,000 20nmol ,000 40nmol ,000 Silencer Select Validated sirna, 1nmol ,000 standard purity 5nmol ,000 20nmol ,000 40nmol ,000 Silencer Select Validated sirna, HPLC purified * 1nmol 4 Silencer Select Negative Control sirna 20nmol ,000 40nmol ,000 Silencer Select Negative Control #1 5nmol ,000 sirna 40nmol , nmol ,000 Silencer Select Negative Control #2 sirna 5nmol ,000 40nmol ,000 Silencer Select GAPDH Positive Control sirna Silencer Select GAPDH sirna (Hs, Mm, Rn) Custom Select sirna 5nmol ,000 40nmol , nmol ,000 Custom Select sirna, standard purity 20nmol ,000 40nmol ,000 Custom Select sirna, HPLC purified 20nmol ,000 40nmol ,000 sirna sirna 3 sirna sirna Silencer Select Human Kinase sirna Library V4 2, Silencer Select Human Phosphatase sirna Library V4 Silencer Select Human GPCR sirna 1, Library V4 Silencer Select Human Nuclear Hormone Receptor sirna Library V4 Silencer Select Human Ion Channel 1, sirna Library V4 Silencer Select Human Protease 1, sirna Library V4 Silencer Select Human Druggable 27,096 9, Genome sirna Library V4-96-well Silencer Select Human Druggable 4,149 1, Genome V4 sirna Ext Set - 96-well Silencer Select Human Extended 31,245 10, Druggable Genome sirna Library V4-96-well Silencer Select Human Druggable 27,096 9, Genome sirna Library V4-384-well Silencer Select Human Druggable 4,149 1, Genome V4 sirna Ext Set well Silencer Select Human Genome sirna 33,510 11, V4 Extension Set well Silencer Select Human Genome sirna Library V4-384-well 64,755 21, nmol 0.5nmol sirna sirna 36 sirna sirna sirna nmol, 0.25 nmol, 1 nmol, 2 nmol sirna Inventoried sirna non- Inventoried sirna 34

35 Ambion In Vivo sirna Ambion In Vivo sirna in vivo RNAisiRNA Silencer Select Silencer Select 33 in vivo RNAi LNA 90% RNAi sirna remaining intact (%) Unmodified sirna: time (h) t 1/2 5 min Ambion In Vivo sirna: t1/2 24 h Ambion In Vivo Pre-designed sirna, 5 nmol ,000 Standard Purity Ambion In Vivo Pre-designed sirna, 250 nmol ,000 HPLC 1 mol ,000 Ambion In Vivo Pre-designed sirna, HPLC In Vivo Ready Ambion In Vivo Custom sirna Standard Purity Ambion In Vivo Custom sirna, HPLC Ambion In Vivo Custom sirna, HPLC In Vivo Ready Ambion In Vivo Custom Designed Standard Purity Ambion In Vivo GAPDH Positive Control sirna Ambion In Vivo GAPDH Positive Control sirna, HPLC In Vivo Ready Ambion In Vivo Factor VII sirna, HPLC In Vivo Ready, 50 nmol Ambion In Vivo Factor VII Positive Control sirna, HPLC In Vivo Ready, 250 nmol Ambion In Vivo Negative Control #1 sirna, 5 nmol Ambion In Vivo Negative Control #1 sirna, HPLC In Vivo Ready, 50 nmol Ambion In Vivo Negative Control #1 sirna, HPLC In Vivo Ready, 250 nmol 250 nmol ,000 1 mol ,000 5 nmol , nmol ,000 1 mol , nmol ,000 1 mol ,000 5 nmol ,000 5 nmol , nmol , nmol , nmol , nmol ,000 5 nmol , nmol , nmol , sirna Ambion In Vivo sirna 8 sirna 90% sirna HPLC TaqMan sirna Assays TaqMan sirna Assays Silencer Select sirna in vivo sirna TaqMan Assays sirna shrna TaqMan sirna Assays, Made-to-Order, S ,000 TaqMan sirna Assays, Made-to-Order, M ,800 TaqMan sirna Assays, Made-to-Order, L 2, ,500 *20µL 35

36 RNA sirna mirna Lipofectamine RNAiMAX Lipofectamine RNAiMAXsiRNA mirna Silencer Select sirna 33 mirvana mirna mimics & inhibitors 43 sirna Invivofectamine 2.0 Invivofectamine 2.0 sirna mirna in vivo sirna 70% mrna 3mg/kg 3A. mrna p53 % remaining expression 120% 100% 80% 60% 40% 20% mrna 0 7mg/kg 7mg/kg 5mg/kg 3mg/kg 1mg/kg p Lipofectamine RNAiMAX Lipofectamine RNAiMAX µl RNAiMAXp53 CTRL FVII sirna complexed with Invivofectamine 2.0 3B. % remaining expression 120% 100% 80% 60% 40% 20% 0 7mg/kg 7mg/kg 5mg/kg 3mg/kg 1mg/kg CTRL FVII sirna complexed with Invivofectamine 2.0 3C. Protein 120% p53, relative to nontransfected nm + Lipofectamine RNAiMAX Scrambled control 10 nm + B 10 nm + T p53 sirna 10 nm + Q 1 nm + Lipofectamine RNAiMAX 1 nm + B 1 nm + T 1 nm + Q Nontransfected 26. Lipofectamine RNAiMAX Lipofectamine RNAiMAX 0.1 ml , ml , ml , ml , ml ,000 activity % remaining FVII 100% 80% 60% 40% 20% 0 CTRL-7mg/kg No treatment FVII sirna- 3mg/kg FVII sirna- 5mg/kg FVII sirna- 7mg/kg 3mg/kg 5mg/kg 7mg/kg Day 2 Day 9 Day 15 Day 22 Day 29 Days after injection 27. Invivofectamine 2.0 Reagent Ambion In Vivo sirna 3A Invivofectamine 2.0 Reagent Factor VII sirna mrna 95% mg/kg TaqMan 348 Factor VII 95% 3C Factor VII sirna mg/kg FVII Invivofectamine 2.0 Reagent Starter Kit* 1mL ,000 Invivofectamine 2.0 Reagent 5mL ,000 *Starter Kit sirna Ambion In Vivo Factor VII sirna 50nmol 36

37 microrna microrna microrna mirna pol precursor mirna mirna 21-23nt mature mirna mirna non-coding RNA mirna mirna mrna 3'UTR mrna mirna Human Precursor mirna Mature mirna 28. mirna (Mature mirna) mirna mirna mirna mirbase mirna 8. mirna mirna mirna mirna mirvana mirna Isolation Kit mirvana PARIS Kit TaqMan mirna ABC Purlfication Kit TaqMan MicroRNA Cells-to-CT Kit TaqMan Array MicroRNA Cards TaqMan Universal PCR Master Mix II Megaplex Primer Pools TaqMan MicroRNA RT Kit TaqMan MicroRNA Assays TaqMan Universal PCR Master Mix II TaqMan MicroRNA RT Kit mirvana mirna mimic mirvana mirna inhibitor Lipofectamine RNAiMAX TaqMan Gene Expression Cells-to-CT Kit TaqMan Gene Expression Assays 37

38 microrna mirvana mirna Isolation Kit RNA RNA microrna mirna small RNA mirvana mirna Isolation Kit mirna RNA mirna Total RNA 250mg Total RNA 30 mirvana mirna Isolation Kit 40 AM ,300 mirvana mirna Isolation Kit without Phenol 40 AM ,000 mirvana PARIS Kit small RNA RNA 2 1 Small RNA RNA mirna 30 small RNA RNA mrna mirna sirna mg 200 small RNA RNA TaqMan mirna ABC Anti-miRNA Bead Capture Purification Kit mirna 75 Human Panel A B 754 mirna mirna Anti-miRNA oligos Dynabeads mirna mirna 10 L 50 L RT-qPCR TRIzol 75 mirna Avg. C t cel-mir-39 expression profile serum sample (50 μl) hsa-mir-16 hsa-let-7a Assay ID 30. TaqMan mirna ABC Purification Ki 50 L hsa-mir-150 hsa-let-7c hsa-mir-135b mirvana PARIS Kit 40 AM , ABC Purification Kit TaqMan mirna ABC Purification ,000 Kit-Human Panel A TaqMan mirna ABC Purification Kit-Human Panel B ,000 38

39 microrna TaqMan MicroRNA RT Kit TaqMan MicroRNA RT Kit TaqMan MicroRNA Assays 40 TaqMan MicroRNA Assays RT TaqMan MicroRNA RT Kit ,300 1, ,800 Megaplex Primer Pools Megaplex RT Primers stem-looped Sanger mirbase mirna mirna RNA Megaplex Primer Pools TaqMan MicroRNA Assays TaqMan MicroRNA Arrays Megaplex PreAmp Primers 1ng RNA Megaplex RT Primers Pool (Rodent) Megaplex RT Primers, Rodent Pool A ,900 Megaplex RT Primers, Rodent Pool B ,900 Megaplex RT Primers, Rodent Pool Set ,000 RT Primers Pools A&B ( & ) Megaplex RT Primers, Rodent Pool B v ,900 Megaplex RT Primers, Rodent Pool Set v3.0 RT Primers Pools A&B ( & ) ,000 Megaplex PreAmp Primers Pool (Rodent) Megaplex PreAmp Primers, Rodent Pool A ,900 PreAmp Megaplex PreAmp Primers, Rodent Pool B ,900 PreAmp Megaplex PreAmp Primers, Rodent Pool Set ,000 PreAmp Primers Pools A&B ( & ) Megaplex PreAmp Primers, Rodent Pool B v ,900 PreAmp Megaplex PreAmp Primers, Rodent Pool Set v3.0 PreAmp Primers Pools A&B ( & ) ,000 Megaplex RT and PreAmp Primers Package (Rodent) Megaplex Primer Pools, Rodent Pools A ,200 RT Primers ( ) PreAmp Primers ( ) Megaplex Primer Pools, Rodent Pools B ,200 RT Primers ( ) PreAmp Primers ( ) Megaplex Primer Pools, Rodent Pools Set ,400 RT Primers Pools A&B ( ) PreAmp Primers Pools A&B ( ) Megaplex Primer Pools, Rodent Pools B v ,200 RT Primers ( ) PreAmp Primers ( ) Megaplex Primer Pools, Rodent Pools Set v3.0 RT Primers Pools A&B ( ) PreAmp Primers Pools A&B ( ) ,400 Megaplex RT Primers Pool (Human) Megaplex RT Primers, Human Pool A ,900 Megaplex RT Primers, Human Pool B Megaplex RT Primers, Human Pool Set RT Primers Pools A&B ( & ) Megaplex RT Primers, Human Pool B v3.0 Megaplex RT Primers, Human Pool Set v3.0 RT Primers Pools A&B ( & ) , , , ,000 Megaplex PreAmp Primers Pool (Human) Megaplex PreAmp Primers, Human Pool A ,900 PreAmp Megaplex PreAmp Primers, Human Pool B PreAmp Megaplex PreAmp Primers, Human Pool Set PreAmp Primers Pools A&B ( & ) Megaplex PreAmp Primers, Human Pool B v3.0 PreAmp Megaplex PreAmp Primers, Human Pool Set v3.0 PreAmp Primers Pools A&B ( & ) , , , ,000 Megaplex RT and PreAmp Primers Package (Human) Megaplex Primer Pools, Human Pools A RT Primers ( ) PreAmp Primers ( ) ,200 Megaplex Primer Pools, Human Pools B RT Primers ( ) PreAmp Primers ( ) Megaplex Primer Pools, Human Pools Set RT Primers Pools A&B ( ) PreAmp Primers Pools A&B ( ) Megaplex Primer Pools, Human Pools B v3.0 RT Primers ( ) PreAmp Primers ( ) Megaplex Primer Pools, Human Pools Set v3.0 RT Primers Pools A&B ( ) PreAmp Primers Pools A&B ( ) , , , ,400 TaqMan MicroRNA Arrays A B Megaplex Primer Pools 39

40 microrna TaqMan MicroRNA Assays TaqMan MicroRNA Assays PCR microrna mirna TaqMan MicroRNA Assays mirna mirna mirna mirna Assays Synthetic mirna Target Relative Detection (%) No. of base differences TaqMan mirnamirna PCR ng RNA 7 TaqMan MicroRNA Assays TaqMan MicroRNA Assays mirna loop mirna RT mirna mirna 5' TaqMan PCR mirna Mature mirna Looped RT primer TaqMan MicroRNA Assays 1let-7a let-7a let-7c 3.7 TaqMan MicroRNA Assays, ,800 Inventoried* mirna RT primer 5X reaction mix (50 / 15 µl ) mirna TaqMan Assay 20X reaction mix (150 / 20 µl ) 150 TaqMan MicroRNA Assays, Made-to-Order, Small mirna RT primer 5X reaction mix (50 / 15 µl ) mirna TaqMan Assay 20X reaction mix (150 / 20 µl ) TaqMan MicroRNA Assays, Made-to-Order, Medium TaqMan MicroRNA Assays, Made-to-Order, Large ,900 2, , , ,500 * Arabidopsis thaliana Caenorhabditis elegans Drosophila melanogaster Made-to-Order Sanger Release 16 mirna Custom TaqMan Small RNA Assays Forward primer TaqMan probe Reverse primer 32. TaqMan MicroRNA Assays TaqMan MicroRNA Assays mirna mirnas Custom TaqMan Small RNA Assays, , Custom TaqMan Small RNA Assays, ,200 2,250 Custom TaqMan Small RNA Assays, 2, ,600 8,700 *20µL 40

41 TaqMan Array MicroRNA Card TaqMan Array MicroRNA Card TaqMan 384 TaqMan mirna 1 Megaplex RT Primers 39 TaqMan MicroRNA Array v2.0 / v3.0 Megaplex PreAmp Primers TaqMan MicroRNA Arrays v2.0 / v3.0 Megaplex Primer Pools Sanger mirbase 5 TaqMan Array Applied Biosystems 7900HT ViiA 7 QuantStudio TaqMan Array mature mirna Human TaqMan Array Human MicroRNA A+B Cards Set v2.0 A array ( ) + B Array ( ) TaqMan Array Human MicroRNA A Cards v2.0 TaqMan Array Human MicroRNA B Cards v2.0 TaqMan Array Human MicroRNA A+B Cards Set v3.0 A array ( ) + B Array ( ) TaqMan Array Human MicroRNA B Cards v3.0 Rodent , , , , ,000 TaqMan Array Rodent MicroRNA A+B Cards Setv2.0 A array ( ) + B Array ( ) TaqMan Array Rodent MicroRNA A Cards v2.0 TaqMan Array Rodent MicroRNA B Cards v2.0 TaqMan Array Rodent MicroRNA A+B Cards Set v3.0 A array ( ) + B Array ( ) TaqMan Array Rodent MicroRNA B Cards v , , , , ,000 mirvana mirna Isolation Kit TaqMan mirna ABC Purification Kit Megaplex RT Primers & TaqMan MicroRNA Reverse Transcription Kit Megaplex PreAmp Primers & TaqMan PreAmp Master Mix Diluted RT product & TaqMan Universal PCR Master Mix II Applied Biosystems QuantStudio 12K Flex ViiA 7 / 7900 HT Fast PCR RT-PCR Expression Suite Software 34. mirna 41

42 Non-coding RNA TaqMan Pri-miRNA Assays mirna TaqMan Pri-miRNA Assays TaqMan Pri-miRNA Assays microrna mirna primary microrna Pri-miRNA microrna Pri-miRNA 1 microrna Drosha mirna mirna stem-loop Dicer RNA Induced Silencing Complex RISC mirna 35. microrna 37. hsa-mir-26 mir-26a-1 mir-26a-2 Pri-miRNA Stemloop Pri-miR-26a-2 TaqMan Pri-miRNA Assay hsa-mir-26a-2 TaqMan Non-coding RNA Assays TaqMan Non-coding RNA Assays TaqMan Non-coding RNA Assays non-cording TaqMan Gene Expression AssaysTaqMan Non-coding RNA TaqMan Non-coding RNA Assays TaqMan PreAmp Master Mix Pre-Amplification 36. TaqMan Pri-miRNA Assays Sanger mirbase Stem-loop 500 Pri-miRNA Sanger mirbase sequence repository stem-loop mirna TaqMan Pri-miRNA Assay TaqMan MicroRNA AssaymiRNA TaqMan Non-coding RNA Assays, S ,000 TaqMan Non-coding RNA Assays, M ,800 TaqMan Non-coding RNA Assays, L 2, ,500 *20µL TaqMan Pri-miRNA Assays, S ,000 TaqMan Pri-miRNA Assays, M ,800 TaqMan Pri-miRNA Assays, L 2, ,500 *20µL 42

43 microrna mirvana mirna mimic & inhibitors mirna mirna mirbase in vivo in vivo ready mirvana mirna mimic mirvana mirna mimic mirna mirna mirna mirna 2 mirna mirvana mirna mimic 5 nmol , nmol ,000 mirvana mirna mimic, in vivo Ready 250 nmol ,000 Customer-Defined mirvana mirna mimic Customer-Defined mirvana mirna mimic, in vivo Ready mirvana mirna mimic Negative Control #1 mirvana mirna mimic Negative Control #1, in vivo Ready mirvana mirna mimic mir-1 Positive Control mirvana mirna mimic mir-1 Positive Control, in vivo Ready mirvana mirna mimic mirbase v nmol , nmol , nmol ,000 5 nmol , nmol , nmol , nmol ,000 5 nmol , nmol , nmol , nmol , nmol each 1 nmol each mirvana mirna inhibitor mirvana mirna inhibitormirna mirna mirna 1 mirvana mirna inhibitor 5 nmol , nmol ,000 mirvana mirna inhibitor, in vivo 250 nmol ,000 Ready Customer-Defined mirvana mirna 5 nmol ,000 inhibitor 20 nmol ,000 Customer-Defined mirvana mirna 250 nmol ,000 inhibitor, in vivo ready mirvana mirna inhibitor Negative 5 nmol ,000 Control #1 20 nmol ,000 mirvana mirna inhibitor Negative 50 nmol ,000 Control #1, in vivo Ready 250 nmol ,000 mirvana mirna inhibitor let-7 5 nmol ,000 Positive Control 20 nmol ,000 mirvana mirna inhibitor 0.25 nmol mirbase v 19 each 1 nmol each Pre-miR mirna Precursor Molecule Pre-miR mirna Precursor Starter Kit 1 AM ,000 Pre-miR mirna Precursors 5nmol AM ,000 Molecule 20nmol AM , nmol Customer-Defined Pre-miR mirna Precursor 20nmol 4 5nmol AM ,000 Pre-miR Negative Control #1 5nmol AM ,000 Pre-miR Negative Control #2 5nmol AM ,000 Cy3 Pre-miR Negative Control 5nmol AM ,000 FAM Pre-miR Negative Control 5nmol AM ,000 Pre-miR hsa-mir-1 mirna Precursor 5nmol AM ,000 Anti-miR mirna Inhibitor Anti-miR mirna Inhibitor 5nmol AM ,000 20nmol AM , nmol Customer Defined Anti-miR mirna Inhibitor 20nmol 4 5nmol AM ,000 Anti-miR Negative Control #1 5nmol AM ,000 Cy3 Anti-miR Negative Control 5nmol AM ,000 FAM Anti-miR Negative Control 5nmol AM ,000 mirna 36 Lipofectamine RNAiMAX Invivofectamine

44 SNP TaqMan SNP 2 TaqMan TaqMan 5' VIC dye FAM dye 3' Q PCR DNA VIC dye FAM dye TaqMan SNP Genotyping Assay 100 DNA 38. TaqMan SNP 39. Allele1 TaqMan VIC Allele2 TaqMan FAM VIC FAM Allele1 Allele2 Allele1 2 44

45 SNP TaqMan Pre-Designed SNP Genotyping Assays TaqMan SNP Genotyping Assays SNP SNP SNP ID SNP csnp 7 SNP 16 SNP 350 SNP 2600 Human TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Small scale 300 1, ,000 TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Medium scale TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Large scale * 25µL 5µL non-human 1,000 5,000 2,400 12, , ,900 TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Small scale 300 1, ,000 TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Medium scale TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Large scale * 25µL 5µL 1,000 5,000 2,400 12, , ,900 Custom TaqMan SNP Genotyping Assays Custom TaqMan SNP Genotyping Assays SNP SNP SNP SNP SNP 6 MNP SNP Human Custom TaqMan SNP Genotyping ,600 Assays, S-scale, Human 1,500 Custom TaqMan SNP Genotyping 1, ,100 Assays, M-scale, Human 5,000 Custom TaqMan SNP Genotyping Assays, L-scale, Human 2,400 12, ,200 * 25µL 5µL non-human Custom TaqMan SNP Genotyping ,600 Assays, S-scale, non-human 1,500 Custom TaqMan SNP Genotyping 1, ,100 Assays, M-scale, non-human 5,000 Custom TaqMan SNP Genotyping Assays, L-scale, non-human 2,400 12, ,200 * 25µL 5µL TaqMan Drug Metabolism Genotyping Assays 220 2,000 TaqMan Drug Metabolism Genotyping ,600 Assays 750 * 25µL 5µL 45

46 SNP SNP TaqMan Genotyping Master Mix TaqMan Genotyping Master Mix PCR GC AT TaqMan Universal Master Mix GC AT 72 Fast SNP TaqMan GTXpress Master Mix TaqMan GTXpress Master Mix Fast PCR SNP SNP TaqMan PCR 50 PCR 50 Tracking Dye 9. PCR 40. TaqMan Genotyping Master Mix GC-rich TaqMan Genotyping Master Mix 94 gdna TaqMan SNP Assay C _10 PCR GeneAmp PCR System HT PCR GC-rich Catjespin D rs12214 dbsnp TaqMan Genotyping Master Mix TaqMan Genotyping Master Mix, ,000 1mL 400 TaqMan Genotyping Master Mix, 10mL 800 4, ,000 TaqMan Genotyping Master Mix, 10mL 2 TaqMan Genotyping Master Mix, 50mL TaqMan Genotyping Master Mix, 50mL 2 TaqMan Genotyping Master Mix TaqMan Genotyping Master Mix * 25µL 5µL 1,600 8,000 4,000 20,000 8,000 40, , , , TaqMan GTXpress Master Mix TaqMan GTXpress Master Mix, 1mL , TaqMan GTXpress Master Mix, 10mL ,000 2,000 4,000 TaqMan GTXpress Master Mix, 50mL 4, ,000 10,000 20,000 TaqMan GTXpress Master Mix, 100mL 2 50mL 8,000 20,000 40, ,000 *PCR 96 Standard Block,0.2µL 25µL 96 Fast Block,0.1µL 10µL 384 5µL 46

47 SNP TaqMan Sample-to-SNP Kit TaqMan Sample-to-SNP Kit DNA TaqMan SNP 1 DNAFast PCR TaqMan GTXpress Master Mix DNA 5 PCR 50 SNP 1 / FTA / / / Buccal swab / Rat Mouse tail / Leaf punch / / FFPE DNA 95 3 DNA PCR Fast PCR TaqMan GTXpress Master Mix PCR TaqMan Sample-to-SNP Kit A. Buccal swab, B. Mouse tail, C. Corn leaf, D. FFPE TaqMan Sample-to-SNP Kit DNA ,000 5mL sample prep, 1mL PCR PCR 80 TaqMan Sample-to-SNP Kit 20mL sample prep, 10mL PCR TaqMan Sample-to-SNP Kit 20mL sample prep, 50mL PCR TaqMan Sample-to-SNP Kit 200mL sample prep, 10mL PCR * PCR 25µL DNA 400 PCR 800 DNA 400 PCR 4,000 DNA 4,000 PCR , , , TaqMan Sample-to-SNP Kit SNP SNP / 47

48 TaqMan Mutation Detection Assays TaqMan Mutation Detection Assays Competitive Allele-Specific TaqMan PCR castpcr MGB TaqMan Mutation Detection Assay 11. Reference Assay Mutant Assay Mutant Allele Assay EGFR_exlqdels_mu* 19 MGB ASP = ASB = MGB LST = TaqMan LSP = EGFR exon19 deletion Wild type Assay Mutant Assay EGFR Reference Assay EGFR Exon19 Deletion Assay Wild type Allele Assay MGB ASP = ASB = MGB LST = TaqMan LSP = Mutation Detector TaqMan Mutation Detection Assay Gene Reference Assay FP = RP = LST = TaqMan ASP: LST: TaqMan LSP: MGB: ASB: MGB FP: RP: TaqMan Mutation Detection Assays ** 150µL ,800 TaqMan EGFR Exon 19 Deletions Assay 1 EGFR 19 1 ** 150µL ,800 TaqMan Mutation Detection Reference Assays 46 ** 150µL ,000 TaqMan Mutation Detection IPC Reagent Kit 1 kit ,300 *20µL ** TaqMan Mutation Detection Assays 48

49 TaqMan Copy Number Assays Copy Number Variations, CNVs CNVs CNVs TaqMan Copy Number Assays CNVs 160 TaqMan CNVs TaqMan Copy Number Assays TaqMan Copy Number Reference Assays PCR 3 4 PCR DNA Copy Number 4 TaqMan Copy Number Assays, 360 assays ,000 Small 720 assays TaqMan Copy Number Assays, 750 assays ,900 Medium 1,500 assays TaqMan Copy Number Assays, Large 2,900 assays 5,800 assays ,600 * 20µL 96 10µL 384 Custom TaqMan Copy Number Assays Custom TaqMan Copy Number Assays TaqMan Copy Number Assays CNVs GeneAssist Copy Number Assay Tool CNVs Custom TaqMan Copy Number Assays, 360 assays ,000 Small 720 assays Custom TaqMan Copy Number Assays, 750 assays ,000 Medium 1,500 assays Custom TaqMan Copy Number Assays, Large 2,900 assays 5,800 assays ,800 * 20µL 96 10µL 384 CopyCaller TaqMan Copy Number Assays CopyCallerTM TaqMan Copy Number Reference Assays TaqMan Copy Number Reference Assays TaqMan Copy Number Assays Custom TaqMan Copy Number Assays RNase P Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2TERT RNase P telomerase reverse transriptase (TERT) gene located on chromosome 5, cytoband 5p15.33 Human TaqMan Copy Number Reference Assay, 750 assays ,200 RNase P 1,500 assays 3,000 assays 6,000 assays ,500 TaqMan Copy Number Reference Assay, TERT 750 assays 1,500 assays 3,000 assays 6,000 assays , ,500 Mouse * TaqMan Copy Number Reference Assay, 750 assays ,200 TFRC 1,500 assays 3,000 assays 6,000 assays ,500 TaqMan Copy Number Reference Assay, TERT 750 assays 1,500 assays 3,000 assays 6,000 assays , ,500 * 20µL 96 10µL 384 Custom Plus TaqMan Copy Number Assays Custom Plus TaqMan Copy Number Assays Custom TaqMan Copy Number Assays SNP TaqMan TaqMan Copy Number Assays * Custom Plus TaqMan Copy Number 360assays ,000 Assays, Small 720assays Custom Plus TaqMan Copy Number 750assays ,000 Assays, Medium 1,500assays Custom Plus TaqMan Copy Number Assays, Large 2,900assays 5,800assays ,800 * 20µL 96 10µL

50 High Resolution Melt HRM High Resolution Melting HRM PCR PCR PCR MeltDoctor HRM MeltDoctor HRM 1 * 2 PCR 3 PCR 4 HRM 5 HRM PCR PCR PCR 3 5 PCR *Only required once every six months HRM PCR 43. HRM MeltDoctor HRM High Resolution Melting (HRM) PCR MeltDoctor HRM SYTO 9 DNA Tm PCR dutp dntp PCR HRM 50

51 MeltDoctor HRM HRM AmpliTaq Gold 360 DNA Polymerase PCR MeltDoctor HRM 2 SYTO 9 MeltDoctor HRM MeltDoctor HRM HRMPCR MeltDoctor HRM 1 500units ,000 AmpliTaq Gold 360 DNA Polymerase GeneAmp dntp Blend MeltDoctor HRM Dye MeltDoctor HRM ,000 5 ml MeltDoctor HRM 2, , ml MeltDoctor HRM 5, , ml MeltDoctor HRM 5, , ml *20µL MeltDoctor HRM MeltDoctor HRM 3 AA, AB, BB 3 DNA MeltDoctor HRM ,000 MeltDoctor HRM MeltDoctor HRM 96 Fast MeltDoctor HRM DNA 44. HRM 3 HeLa, Raji, and Jurkat HRM p bp PCR 3 HRM GC, GG, CC HRM MeltDoctor HRM Applied Biosystems 7500 Fast PCR Applied Biosystems HRM Software v2.0 HRM PCR BigDye Terminator v1.1 Cycle Sequencing Kit Applied Biosystems 3130 MeltDoctor HRM , Fast MeltDoctor HRM ,000 MeltDoctor HRM 20 1mL ,000 HRM HRM HRM PCR 51

52 TaqMan Protein Assays Build Your Assay 90 min Prepare Sample 30 min Binding 60 min - TaqMan Protein Assay Open Kit Protein Quant Sample Lysis Kit Protein Expression Sample Lysis Kit Ambion PARIS Kit TaqMan Protein Assay TaqMan Protein Assay Open Kit TaqMan Protein Assay Kit Ligation/ Inactivation 25 min TaqMan Fast Real-Time PCR 40 min Data Analysis DNA TaqMan Protein Assay Core Reagents Kit with Master Mix Universal TaqMan Assay PCR TaqMan PCR mrna mirna ProteinAssist Software v TaqMan Protein Assays 52

53 TaqMan Protein Assays TaqMan Protein Assays TaqMan 5 mrna mirna TaqMan Protein Expression Core Reagents Kit with Master Mix TaqMan PCR TaqMan Protein Assays Kit TaqMan Protein Assays Open Kit TaqMan Protein Assay Core Reagent Base Kit DNA Ligase, Protease, Ligase PCR / TaqMan Protein Assays Fast Master Mix ph PCR TaqMan Protein Assays Kit (hesc ) hicam1 hcstb TaqMan Protein Assay Kit hcstb ,600 TaqMan Protein Assay Kit hicam ,600 TaqMan Protein Assay Open Kit TaqMan Protein Assay 2 TaqMan PCR mrna mirna TaqMan Protein Assays Open Kit TaqMan Protein Assays Oligo Probe Kit TaqMan Protein Assays Buffer Kit 4, ,300 TaqMan Protein Assays Oligo Probe Kit 4, ,600 5'Prox-Oligo 200nM, 50µL 3'Prox-Oligo 200nM, 50µL TaqMan Protein Assays Buffer Kit Antibody Dilution Buffer, 1mL Assay Probe Strage Buffer, 1mL Assay Probe Dilution Buffer, 7mL Lysate Dilution Buffer, 25mL 4, ,900 TaqMan Protein Expression Core ,600 Reagents Kit with Master Mix TaqMan Protein Expression Core Reagents Base Kit TaqMan Protein Assays Fast Master Mix TaqMan Protein Expression Core Reagents Kit with Master Mix TaqMan ProteinExpression Core Reagents Base Kit TaqMan Protein Assays Fast Master Mix ,700 TaqMan Protein Assays Core Reagents ,500 Base Kit ,400 TaqMan Protein Assays Fast Master Mix , ,600 53

54 Protein Expression Sample Preparation Kit TaqMan Protein Assay Kit TaqMan Protein Assay Open Kit 10,000 50,000 Protein Quant Sample Lysis Kit 50,000 Protein Expression Lysate Control Kits TaqMan Protein Assays Protein Expression Lysate Control Kit (Raji) hcstb hicam1 Protein Expression Lysate Control Kit ,600 Raji ProteinAssist TaqMan Protein Assays ProteinAssist Protein Expression Sample Preparation Kit Protein Quant Sample Lysis Kit 10,000 50,000 50,000 Lysis Yes No TaqMan Protein Assays 500 cells/µl 2,500 cells/µl TaqMan Protein Assay 1:2 1:10 Protein Expression Sample Prep Kit cell counting 1, ,800 Protein Quant Sample Lysis Kit cell counting or total protein quant 25 ml ,300 54

55 PCR Protein Thermal Shift PCR PCR 1 10 * PCR * 1 Protein Thermal Shift , Protein Thermal Shift ,000 Protein Thermal Shift Dye Kit 2, ,000 Protein Thermal Shift 2, ,000 Starter Kit ,000 Protein 80,000 Protein + Ligand d /dt 60,000 40,000 20, ,000 10,000 5, , ~7oC Tm Difference StepOne /StepOnePlus 7500 / 7500 Fast ViiA 7 / QuantStudio 12K Flex PCR StepOne / StepOnePlus v / 7500 Fast v2.0.4 ViiA 7 v1.0 QuantStudio 12K Flex 46. Tm 30 PCR 47. Protein Thermal Shift TM 55

56 Well Clear Optical Reaction Plate ( ) 96-Well Reaction Plate ( N / ) + + Adhesive Film ( / ) Adhesive Film ( / ) QuantStudio 12K Flex PCR Applied Biosystems ViiA 7 PCR QuantStudio 12K Flex ViiA 7 96-Well Reaction Plate ( N / ) Optical Tubes ( N ) Fast 8-Tube Strip, 0.1mL ( ) Fast 96-Well Reaction Plate ( / ) + or Optical 8-Tube Strip and Fast Reaction Tube with Cap, 0.1mL ( ) Optical Caps (Flat Cap) ( ) Tray / Retainer Set ( ) ( ) Well Tray for VeriFlex TM Blocks ( ) 96-Well Tray for VeriFlex TM Blocks ( ) Adhesive Film ( / ) + Optical Caps (Flat Cap) ( ) Optical 8-Cap Strip ( ) 384-Well Clear Optical Reaction Plate ( ) + Adhesive Film ( / ) Applied Biosystems 7900HT Fast PCR 7900HT 96-Well Reaction Plate ( N / ) 96-Well Reaction Plate ( N / ) Fast 96-Well Reaction Plate ( / ) Adhesive Film ( / ) Optical 8-Cap Strip ( ) Adhesive Film ( / ) + Compression Pad ( ) 7500 Fast Fast 96-Well Reaction Plate ( / ) Fast 8-Tube Strip, 0.1mL ( ) + + Adhesive Film ( / ) Optical 8-Cap Strip ( ) Applied Biosystems 7500 Fast / 7500 PCR Well Reaction Plate ( N / ) + Adhesive Film ( / ) 7300 Optical Tubes or Optical 8-Tube Strip Optical 8-Cap Strip Optical 8-Tube Strip ( N ) and ( ) + ( ) StepOne Plus Fast 96-Well Reaction Plate ( / ) Fast 8-Tube Strip, 0.1mL ( ) Fast Reaction Tube with Cap, 0.1mL ( ) Adhesive Film ( / ) 96-Well Tray for VeriFlex TM Blocks ( ) 96-Well Tray for VeriFlex TM Blocks ( ) + Optical 8-Cap Strip ( ) StepOnePlus TM /StepOne TM PCR StepOne Fast Optical 48-Well Reaction Plate ( ) Fast 8-Tube Strip, 0.1mL ( ) Well Optical Adhesive Film ( / ) Fast 48-Well Tray ( ) + Optical 8-Cap Strip ( ) Fast Reaction Tube with Cap, 0.1mL ( ) + Fast 48-Well Tray ( ) ABI PRISM Well Reaction Plate ( N / ) 96-Well Reaction Plate ( N / ) Optical Tubes ( N ) or Optical 8-Tube Strip and ( ) Adhesive Film ( / ) Optical 8-Cap Strip ( ) Tray / Retainer Set ( ) + + Compression Pad ( ) Optical 8-Cap Strip ( ) 56

57 Fast 96-Well 7900HT Fast 96 / 7500 Fast / StepOnePlus ViiA 7 / QuantStudio Fast 96 MicroAmp Fast 96-Well Reaction ,200 Plate 0.1mL MicroAmp Fast Optical 96-Well ,000 Reaction Plate with Barcode 0.1mL , HT / 7700 / 7500 / 7500 Fast / 7300 / 7000 / 5700 / StepOnePlus / ViiA 7 / QuantStudio MicroAmp Optical Adhesive Film , ,500 MicroAmp Adhesive Film Applicator , Well 7900HT 96 / 7700 / 7500 / 7300 / 7000 / 5700 ViiA 7 / QuantStudio Standard 96 MicroAmp Optical 96-Well Reaction Plate 10 N ,000 MicroAmp Optical 96-Well Reaction Plate ,800 with Barcode MicroAmp Optical 96-Well Reaction Plate ,000 with Barcode MicroAmp Optical 96-Well Reaction Plate with Barcode MicroAmp Optical 96-Well Reaction Plate ,000 with Barcode and Optical Caps MicroAmp Optical 96-Well Reaction Plate with Barcode MicroAmp Optical 8-Cap Strip Well Compression Pad 7900HT* / 7700 / 7000 / Optical Adhesive Film+Compression Pad * Fast TaqMan Low Density Array Compression Pad MicroAmp Optical Adhesive Film Kit ,500 MicroAmp Optical Adhesive Film 20 1 MicroAmp Optical Film Compression Pad HT 96 * / 7700 / 7500 / 7500Fast / 7000 / 5700 / StepOnePlus / StepOne / ViiA 7 / QuantStudio * 384 TaqMan Array Optical Cap MicroAmp Optical 8-Cap Strip Flat ,800 N Well 7900HT 384 / ViiA 7 / QuantStudio 384 MicroAmp Optical 384-Well Reaction ,000 Plate with Barcode MicroAmp Optical 384-Well Reaction ,000 Plate with Barcode MicroAmp Optical 384-Well Reaction Plate with Barcode MicroAmp Optical 384-Well Reaction 1, ,000 Plate with Barcode Optical 384-Well Reaction Plate 1000 Code 128 MicroAmp Optical 384-Well Reaction Plate 1, ,000 Optical 384-Well Reaction Plate / 7500 / 7000 / 5700 / ViiA 7 / QuantStudio Standard 96 MicroAmp Optical 8-Tube Strip , mL MicroAmp Optical Tube without Cap 0.2mL 2,000 N ,000 57

58 Fast 7500Fast / StepOnePlus / StepOne / ViiA 7 / QuantStudio Fast 96 MicroAmp Fast 8-Tube Strip , mL & 7700 / 7000 / 5700 MicroAmp 96-Well Tray/Retainer Set ,000 Fast StepOnePlus / StepOne MicroAmp Fast Reaction Tube 1, ,000 with Cap 0.1mL StepOne 48-Well StepOne 48 MicroAmp Fast Optical 48-Well ,000 Reaction Plate MicroAmp Optical 48-Well Adhesive Film StepOnePlus StepOnePlus , ,000 MicroAmp Fast 48-Well Trays ,000 MicroAmp 48-Well Base Adaptor ,800 MicroAmp 96-Well Tray for VeriFlex ,900 Blocks *StepOnePlus / StepOne MicroAmp Splash Free 96-Well Base ,500 MicroAmp 96-Well Support Base * 13,800 Compression Pad 7900HT* / 7700 / 7000 / Optical Adhesive Film Compression Pad * Fast TaqMan Low Density Array Compression Pad MicroAmp Optical Film Compression ,500 Pad ViiA 7 / QuantStudio ViiA 7 / QuantStudio Standard 96 MicroAmp 96-Well Tray/Retainer Set ,200 for Veriti System ViiA 7 / QuantStudio Fast 96 MicroAmp 96-Well Tray for VeriFlex ,900 Blocks LAMP for GeneAmp 5700 and ,000 ABI PRISM 7000 System LAMP for Applied Biosystems 7300/7500 / ViiA 7 System ,000 58