*, 1 1 Selection of superior Frankia spp. for rapid cultivation of Alnus hirsuta seedlings Maki Saito *, 1 and Mikio Hasegawa 1 4 2 4 28 9 30 cm 30 cm 70 % 5 Abstract: In order to develop the rapid cultivation of potted seedlings of Alnus hirsuta, we selected Frankia spp. favorable for the growth of A. hirsuta. The growth of A. hirsuta seedlings inoculated with suspension of root nodules of the species for two years after germination was different among the root nodule suspensions derived from natural seedlings collected from different four sites. Using the root nodule suspensions re-derived from the seedlings with the best and worse growth-performances revealed by the experiments, repeatable results were obtained, where the average size of the 28 potted seedlings with the best growth-performance exceeded 30 cm in the middle of September and the proportion of seedlings higher than 30 cm was about 70 %. This study demonstrated that A. hirsuta seedlings grew to the size suitable for shipping, for about five months, by the inoculation of Frankia spp. with high growth promoting effects as selected in this study, into germinated seedlings. Keywords: labor saving, local planting stocks, low cost, potted seedlings, revegetation plants Alnus hirsuta Frankia 2001 1992, 1994 Yamanaka et al. 2005 1995 2009 2009 DNA * E-mail: saito@fes.pref.toyama.jp 1 Forestry Research Institute, Toyama Prefectural Agricultural, Forestry & Fisheries Research Center, 3 Yoshimine, Tateyama-machi, Nakaniikawa-gun, Toyama 930-1362, Japan 2012 6 22 2012 9 1 7
2006 30 cm 1 500 mg/l 440 mg/l 56 cm 27 cm 25 cm 2 2010 2 10 2007 10 A B C D 4 30 50 cm 3 5 1 1 56 cm 27 cm 25 cm 2007 10 2 4 2008 4 128 24 cm 3 2009 3 60 3 1.0 g/l 1 2mL 6 21 460 mg/l 1 A 690 m B 810 m C 1010 m D 1100 m 1180 m 1010 m 670 m 1100 m 2010 4 22 3 3 1.0 g/l 1 4 1 1 2 ml 6 18 28 12 cm 700 ml 8
1 1 9 12 4 2 2 1 1 2 4 3 2 6 18 80 4.6 2 1 2.09 1.86 1 4 2 A B A 1% B 5% 2 % 95 80 84.2 90 70 77.8 87 4 4.6 1 F P -9 ** 5169.8 3 1723.2 19.51 2.3 10 227.8 1 227.8 2.57 0.11 n.s. 627.6 3 209.2 2.36 0.07 n.s. 6261.1 72 88.3 ** 1 % n.s. 9
9 13 31.4 cm 30 cm 69.2% 5 6 3 10 4 35.5 cm 84.6 12 30 cm 3 30 cm 9 6 9 13 10 4 11 1 % 34.6 69.2 84.6 84.6 5 3 2 A 2 B 6 1 A 1 B C 2010 9 13 4 1 % Rhizobium 10
1989 1981 1985 1987 Schubert et al. 1978 1999 1989 1995 2009 Rhizobium 4 2010 9 28 30 cm 30 cm 1 30 cm 2011 4 5 1 8 30cm 90 4 5 1994 0 2 mm 100 10 mm 20 50 ph 4.0 1982 1977 Rhizobium pf 2.2 2.8 1982 pf 5 2 3 11
1989 8. 64: 79-82 1981. 52: 114-118 1992. 63: 325-331 1994 13-3-2. 65: 392-399 2006.,.,, pp 117-128 1985. 56: 292-299 1987. 58: 291-292 2009. 35: 332-337 2009. 91: 173-177 (1982) Fusarium oxysporum. 22: 19-26 Schubert KR, Jennings NT, Evans HJ (1978) Hydrogen reaction of nodulated leguminous plants. II. Effects on dry matter accumulation and nitrogen fixation. Plant Physiology 61: 398-401 1999 En-b0-1-2. 68 2 : 36-37 1982. 17: 151-159 2001.,.,, pp 67-74 Yamanaka T, Akama A, Li C-Y, Okabe H (2005) Growth, nitrogen fixation and mineral acquisition of Alnus sieboldiana after inoculation of Frankia together with Gigaspora margarita and Pseudomonas putida. Journal of Forest Research 10: 21-26 1995. 77: 269-271 1989 19 USDA. 64: 1429-1435 1977. 23: 6-13 12