VOL. 30 S-3 CHEMOTHERAPY imeumoniae, Serratia marcescens, Proteus sp,
CHEMOTHERAPY DEC. 1982 Table 1 Antibacterial spectra of T-1982, CTT, CMZ, CTX, CPZ and CEZ 106 CFU/ml Note: P; Peptococcus, S; Streptococcus, G; Gaffkyc, Ps; Peptostreptococcus
VOL. 30 S-3 CHEMOTHERAPY Table 2 Antibacterial spectra of T-1982, CTT, CMZ, CTX, CPZ and CEZ 106 CFU/ml Note: B; Bacteroides, F; Fusobacterium, E ; Eubacterium, P; Propionibacterium C; Clostridium, *V; Veillonella, **V; Vibrio
CHEMOTHERAPY DEC. 1982 Table 3 Antibacterial activity of T-1982 against anaerobic bacteria Table 4 Antibacterial activity of T-1982 against anaerobic bacteria Note: Sensitivity test media; Brucella blood agar Incubation; 24 hours in an anaerobic cham - ber P; Peptococcus, S; Streptococcus, G; Gaffkya Ps; Peptostreptococcus ; 24 hours in an anaero- (Difco), Incubation bic chamber Note: Sensitivity test media ; Brucella blood agar B ; Bacteroides, F ; Fusobacterium **V ; Vibrio,, *V ; Veillonella, E; Eubacterium, P ; Propionibacterium, C ; Clostridium
VOL.30 S-3 CHEMOTHERAPY Table 5 Antimicrobial activity of T-1982 and other three antimicrobials against Bacteroides fragilis (26 strains) Table 6 Antimicrobial activity of T-1982 and other three antimicrobials against Peptococcus magnus (21 strains) Table 7 Antimicrobial activity of T-1982 and other three antimicrobials against Peptococcus asaccharolyticus (8 strains)
CHEMOTHERAPY DEC. 1982 Table 8 Antimicrobial activity of T-1982 and other three antimicrobials against Peptostreptococcus sp.*(17 strains) *Ps. parvulus, Ps. anaerobius, Ps. micros, S. constellatus, S. intermedius, S. morbillorum, Peptostreptococcus sp. Table 9 Antimicrobial activity of T-1982 and other three antimicrobials against Veillonella parvula (14 strains) Table 10 Antimicrobial activity of T-1982 and other three antimicrobials against Clostridium difficile (20 strains)
CHEMOTHERAPY Fig. 1 Development of resistance of Bacteroides fragilis (GAI-0562) to T-1982, LMOX and CEZ Fig. 3 Ĉ-lactamase activity of Bacteroides fragilis (GAI-0830) 5.30 U/mg of protein Hydrolysis of CEZ=100 Fig. 4 Ĉ-lactamase activity of Bacteroides fragilis (GAI-0548) Fig. 2 Development of resistance of Bacteroides fragilis (GAI-0548) to T-1982, LMOX and CEZ 0.85 U/mg of protein Hydrolysis of CEZ=100
CHEMOTHERAPY DEC. 1982 Fig. 5 j9-lactamase activity fragilis (GAI-0556) of Bacteroides Fig. 8 Morphological changes at various concentration of T-1982, LMOX and CEZ Fig. 6 P-Lactamase activity of Bacteroides fragilis (GAI-0511) Fig. 9 Morphological changes at various concentration of T-1982, LMOX and CEZ Fig. 7 /9-Lactamase activity fragilis (GAI-0763) of Bacteroides
CHEMOTHERAPY Fig.10 Appearance of Clostridium difficile in caecum contents of mice administrated various cephem-antibiotics Table 11 In vivo activity of T-1892 against Bacteroides fragilis (GAI-0558) B. fragilis; 108 CFU/mouse i. p. Grade of recovery of B. fragilis; to Table 12 In vivo activity of T-1982 against Bacteroides fragilis (GAI-0558) Medication: dose; 2 mg/day, route; S. C., term; 7 days *4+: 104 or more/plate, 3+: 104 `103/plate, 2+: 103 `102/plate, 1+: 102 `10 /plate,-: no growth B. fragilis; 108 CFU/mouse i. p. Grade of recovery of B. fragilis; -FFE to
CHEMOTHERAPY
CHEMOTHERAPY ANTIBACTERIAL ACTIVITY OF T-1982 AGAINST ANAEROBES KUNITOMO WATANABE, MIDORIISONO, MAKOTO AOKI TOYOKO KOBAYASHI and KAZUE UENO Institute of Anaerobic Bacteriology, Gifu University, School of Medicine The antibacterial activity of T-1982, a new cephamycin antibiotic, was studied. The following results were obtained. 1) The antibacterial spectrum of T-1982 against anaerobes was almost conformable to that of cefmetazole and cefotetan. Its antibacterial activity against B. fragilis was slightly more potent than that of cefmetazole and cefotetan, with the MIC of 1.56-6.25ƒÊg/ml. Against anaerobic cocci, T-1982 was less active than cefazolin, cefotaxime and cefoperazone but comparable to cefmetazole and cefotetan. The activity was weak against B. thetaiotaomicron, E. lentum, C.difficile and C. rarnosum. 2) Like cefmetazole and cefotetan, T-1982 was very stable to ƒà-laatamase produced by B. fragilis. 3) In the bouillon containing T-1982 (at the MIC), B. fragilis was almost completely lyzed and filamentation was scarcely observed. 4) In the broth containing T-1,982, resistance of B. fragilis was gradually increased with the passage of culture. 5) Following s.c. administration of T-1982 at a dose of 2 mg for 7 days in mice, abnormal growth rate of (C. difficile was 80% in the,cecum, although the number of organisms detected was extremely small. 6) T-1982 exhibited potent in vivo antibacterial activity against B. fragilis intraperitoneally inoculated in mice.