Importance of timing for an intake of protein/amino acids nutrients combined with exercise Challenge in Japan after studying in Denmark Masao Mizuno, PhD, Prof. Dept. of Health and Physical Education Faculty of Education
Denmark 6º Esbjerg Copenhagen 4º
Phenylalanine as a marker for muscle proteolysis 筋タンパク質分解マーカー Amino acids Amino acids Synthesis 合成 Uptake 取込 Release 放出 Breakdown 分解 (Okamura et al. Am J Physiol, 272, E123-E13, 1997)
Resistance training in elderly subjects
Mean fiber transverse area of the thigh 大腿外側広筋の筋線維横断面積 6 5 Training effects combined with protein intake タンパク質摂取と組み合わせたトレーニング効果 Cross-sectional area of the thigh 大腿四頭筋の横断面積 m 2 cm 2 Nm * wk 12 wk 7 65 * wk 12 wk 2 Maximal knee extension force 最大膝伸展力 * wk 12 wk 4 6 15 3 55 2 5 1 1 45 Immediate feeding 2hrs Delayed delayed feeding 運動直後 運動 2 時間後 n = 7 n = 6 4 Immediate feeding Delayed feeding Immediate 2hrs delayed 運動直後 運動 2 時間後 n = 7 n = 6 Mean age 74 yr, 13 males 平均年齢 74 歳男性 13 名, 12-week resistance training (3 times/wk) 12 週間の筋力トレーニング ( 週 3 回 ) Protein 1g, CHO 7g, Fat 3g タンパク質 1g 糖質 7g 脂肪 3g 5 Immediate feeding Delayed feeding Immediate 2hrs delayed 運動直後 運動 2 時間後 n = 7 n = 6 (Mean±SD, * P<.5) (Esmark et al. J Physiol, 535.1, 31-311, 21)
Muscle metabolism marker 筋タンパク質代謝マーカー Amino acids Amino acids Synthesis 合成 Uptake 取込 Release 放出 Breakdown 分解 (Okamura et al. Am J Physiol, 272, E123-E13, 1997)
Actual video-clip of the experiment 実験の様子
Plasma BCAA concentrations 血漿 BCAA 濃度 (mean±sd) µmol L -1 µmol L -1 8 8 6 6 4 4 2 Protein CHO 2 BCAA CHO -5 1 25 4 55 7 85 1 115 13 BCAA-P16-A BCAA-Cont-A BCAA-P16-V BCAA-Cont-V Healthy elderly sedentary 健常高齢非鍛錬者群 (n=9) -5 1 25 4 55 7 85 1 115 13 BCAA-P18-A BCAA-Cont-A BCAA-P18-V BCAA-Cont-V Healthy young sedentary 健常青年非鍛錬群 (n=8) (Mizuno et al. FASEB J, 19: 133.2, 25) (Mattsumoto et al. Int J Sports Med, 28, 531-538, 27)
Phenylalanine balance over the lower limb 下肢でのフェニルアラニン バランス (mean±sd) µmol min -1 1,5 1,5 -,5-1 -1,5-2 -2,5 µmol min -1 1,5 1,5 -,5-1 -1,5-2 -2,5 Release -3-5 1 25 4 55 7 85 1 115 13 min Phe-Placebo Healthy elderly sedentary 健常高齢非鍛錬者群 (n=9) -3-5 1 25 4 55 7 85 1 115 13 Phe-IP Phe-NP Healthy young sedentary 健常青年非鍛錬群 (n=8) (Mizuno et al. FASEB J, 19: 133.2, 25) (Mattsumoto et al. Int J Sports Med, 28, 531-538, 27)
Phenylalanine balance over the lower limb 下肢でのフェニルアラニン バランス (mean±sd) µmol min -1 1,5 1,5 * ** * µmol min -1 1,5 1,5 * Uptake -,5 -,5-1 -1-1,5-1,5-2 -2,5-2 -2,5 Release -3-5 1 25 4 55 7 85 1 115 13 Phe-Protein Phe-Placebo Healthy elderly sedentary 健常高齢非鍛錬者群 (n=9) min -3-5 1 25 4 55 7 85 1 115 13 Phe-P18 Phe-IP Phe-NP *P<.5, **P<.1, vs Placebo Healthy young sedentary 健常青年非鍛錬群 (n=8) (Mizuno et al. FASEB J, 19: 133.2, 25) (Mattsumoto et al. Int J Sports Med, 28, 531-538, 27)
Effects of protein/bcaa intake タンパク質 分岐鎖アミノ酸摂取の効果 Moderate bicycling exercise induced skeletal muscle proteolysis in both young and elderly untrained healthy individuals. 軽強度な持久的運動は 非鍛錬で健康な青年と高齢者両群において骨格筋タンパク質の分解を誘発させた As a result of a single protein/bcaa intake, exercise-induced skeletal muscle proteolysis was suppressed in both elderly and young groups. 単回 BCAA 摂取の結果 運動誘発性の骨格筋タンパク質分解は 青年と高齢者の両群において抑制された Due to daily diets a blood level of circulating protein/bcaa is kept constant, thus a timing of nutrient intakes appears to be of importance for suppressing exercise-induced proteolysis. 日常の食事により循環 BCAA の血中レベルは一定に保たれているため BCAA/ タンパク質の摂取タイミングが運動誘発性筋タンパク質分解の抑制にとって重要である
Hokkaido University 北海道大学
Effects of bowling combined with milk intakes 健康ボウリングと牛乳飲用併用の効果 Protein 17. g CHO 24.3 g Fat 2.3 g 348 kcal Fatigue? Cognitive function? Immune function? Upper respiratory tract Inflammation? Protein g CHO 31. g Fat g 125 kcal
Subjects( 研究対象者 ) Methods subjects & drinks 方法 研究対象者と飲料 Age Height Weight M 7 F 12 65 yrs (56-76) 159 cm (147-173) 59 kg (48-81) Milk M 2 F 6 65 yrs (61-69) 155 cm (147-163) 54 kg (5-63) Sport-drink M 5 F 6 65 yrs (56-76) 161 cm (147-173) 63 kg (48-8) Drinks( ゲーム時の飲料 ) Milk 5ml (Protein:17.g,Fat:2.3g,CHO:24.3g, 348 kcal) Sport-drink 5ml (Protein: g, Fat:g, CHO:31g,125 kcal)
Methods Experimental protocol 方法 実験プロトコル Bowling league match Subjects n=36 Milk group (n=8) Sport-drink group (n=11) Physical activity Milk consumption Saliva makers Upper respiratory tract inflammation Hearing Questionnaire April September 214 Oct - December January March 215
Physical activity levels in daily life 日常の身体活動量 (mean±sem) Mild intensity High intensity 28 28 Active hours per week 24 2 16 12 8 Active hours per week 24 2 16 12 8 4 4 Milk 分類 Sport-drink 1 (n=8) (n=11) Milk 分類 Sport-drink 1 (n=8) (n=11)
Daily milk consumption 日常の牛乳摂取量 (mean±sem) 4 Total 4 Home Amount per week (ml) 35 3 25 2 15 1 * Amount per week (ml) 35 3 25 2 15 1 + 5 5 Milk 分類 Sport-drink 1 (n=8) (n=11) Milk 分類 Sport-drink 1 (n=8) (n=11) *:vs 牛乳, p<.5 *:vs 牛乳, p=.52
Saliva IgA concentration 唾液 IgA 濃度 (mean±sem) 16 14 12 μg/ml 1 8 6 4 2 分類 1 Milk Sport-drink (n=8) (n=11)
Plevalence for upper respiratory tract inflammation 上気道感染炎の罹患状況 (mean±sem) Average number 2 1.5 1.5 * 1.3.4 Milk 分類 1 Sport-drink No. of persons 2 out of 8 persons 9 out of 11 persons *:vs 牛乳,p<.5
Effects of milk intake on upper tract inflammation 牛乳摂取の上気道感染炎へ及ぼす効果 Regular physical activity in combination with milk intakes may prevent the onset of acute upper respiratory tract inflammation in elderly individuals during the winter. 牛乳飲用を併用した定期的な身体活動は 急性上気道感染炎への罹患を抑制する効果を有することが示唆された. The intake timing of protein nutrients appears to be also important for immune function preventing acute upper respiratory tract inflammation. The mechanism underlying the preventing process is, however, still unclear. タンパク質栄養の摂取タイミングは 急性上気道感染炎を抑制する免疫機能において重要であることが推察されるが この抑制機序については未解明であると考えられる.