I
3 2 4 2. 4 2. 2 4 2. 3 4 2. 4 4 2. 5 4 2. 6 4 2. 7 5 2. 8 5 2. 9 5 3. 8 3. 8 3.2 8 3.3 0 3.4 0 3.5 4 3.6 6 3.7 7 3.8 Lagmur 22 3.9 Freudlch 26 3.0 29 3. 30 3.2 36 4. 37 4. 37 4.2 37 4.3 39 4.4 4 5. DNA 44 5. 44 5.2 44 5.3 44 5.4 DNA 44 5.5 45 5.6 46 6. 48 6. 48
6.2 48 6.3 48 7. 50 7. 50 7.2 50 7.3 GCMS 53 7.4 54 56
.
2. 2. 2.2 : 4 5 : 4 5 2.3 67 6 2.4 A A 2.5 2 3 2.6 2 2 2.7 7 5 3 3 7 5
2.8 a) b) a 2.9 A4
3 4 5 6 7). 2. 3. 4. 5. [ ][ ] Cp [Jmol K ], Cp =.98 Jmol K 6. 7. 8. 9. Excel
, A4 A 5 TK03500 B6 67
3. 3. C [mol] 3. m 3 40mol/ m 3 40m 3 60kg mol/ m 3 g mol/g g [mol/g] g + V[m 3 ] C 0 [mol/ m 3 ] : W () (C 0 -C)V (2) W(C 0 -C)V (3) C C V = 0 V = ( C C0 ) (4) W W
C kg : [mol/g] V (3) W V mol / g) = ( C C W 2 C 40 3 ( 0 40 ) = ( C 40) 60-2/3 C C 0 Lagmur KC = + KC V = ( C C0 ) W [mol/m 3 ] C 0 -V/W V C 0 W C 5 0.4 mol/ 0.02 mol/ (4) C W= V=00cm 3 C 0 0.52mol/dm 3 dm 3 3 = 0.N 0. C=0.478mol/dm 3 [mol] (4)
V = ( C C0 ) W = () [mol] C 0 [mol/m 3 ] V[m 3 /hr] w[g] C C - [mol/m 3 ] [mol/g] C [mol/m3] [mol/g]lagmur K K8.6 0.003262 EPS EPS0.0000 3.3 Freudlch Lagmur Lagmur 3.4 000ml 500ml 500ml 5
52ml 25ml 20ml 00ml 50ml N/0N/00-NaOHN/0-HCl 2 78 28ml 000ml 000ml N2- N2-500ml 000ml N4N8N6N32 500ml 00ml g 00ml 20 2 N0-,N00-000ml N0- N2- N4-2mlN8- N6-5mlN32 5ml N32 N00 3 2 N2 N0- N0- N2- N4-2mlN8- N6-5mlN32-5ml N32 N00
28ml 000ml 000ml N2- N2-500ml 000ml N4-N8-N6- N32-500ml N0-,N00-3 3 3 3 v v' x = N f 000 000 (3.3) x [mol/] v [m] NNaOH [N] fnaoh [-] v NaOH [m] mol/=n
(3.3) v 2 m, NaOH N = 0. N NaOH f = 0.998, NaOH v = 9.85 m (3.3) x[mol/] v v' x = N f 000 000 v' x = N f v 9.85 = 0. 0.998 = 0. 492 mol/ ( x C C 0 ) 2 00m g 00ml g g 00ml 00ml 00ml 20 2
2 Lagmur Freudlch C0 C 00 = w 000 (3.4) [mol/g] C [mol/] C [mol/] w [g] 3.5 vs C C vs C l vs l C 3 Excel
g w[g] Table Table w [g] 23 2 Table 6 Fg. g [mol/g] C 0 C b w w [mol/] ( C C ) = 0 b [mol/] [g] [mol/g] 00 000 Fg. C b =0.462C 0 =0.4894w=.0300 00 = ( 0.4894 0.462) = 0. 0265.0300 000 Table6 23 2 Table 6 Fg. C b C b =
3.6 WdowsXP OSWdows98, 2000, NT C Excel Excel A A B A A Eter 5 Excel 6 Excel B5 C A B C fx =A+B Eter C 6 Excel C Excel AA3 Delete
A5A9 C 0 mol/dm 3 B5B9 C mol/dm 3 E5E9 W[g] C5 fx (A5 B5)0.E5 8 Excel [mol](4) C 5C9 V = ( C C0 ) W Excel C 5 fx (A5 B5)0.E5 Eter C 6C9 00cm 3 C mol/dm 3 00cm 3 0.dm 3 V=0. dm 3 Excel ad_gra.xls Excel ad_gra.xls 3.6 Lagmur Lagmur
Lagmur Lagmur Excel ad_gra.xls Excel ad_gra.xls USB
3.5 FD ad_gra.xls ad_gra.xls ad_gra.xls ad_gra.xls Excel
4 0 Excel ad_gra.xls ad_gra.xls C b [mol/l] C [mol/g]b,c 4 C b / Excel D5 =B5/C5 D6D9
C b [mol/l] [mol/g] C b [mol/l] C b /[g/l] Excel Excel sheet Graph2 Graph Graph2 Graph C b [mol/l] [mol/g] Graph2 C b [mol/l] C b /[g/l] Graph2Lagmur C b [mol/l] C b [mol/l] [mol/g] C b /[g/l] Lagmur Lagmur K Excel Excel ad_gra.xls Graph2 Graph2Excel Graph Graph2
3.8 Lagmur Lagmur K Graph2 C b [mol/l] C b /[g/l]excel R ax+b OK OK
OK 22
22 C8 D8 Graph2 C b [mol/l] C b /[g/l] Excel 438.6526.54 438.65 26.54 Lagmur K 3.6 Lagmur C ( + KC ) C KC = K = C = C = =( K) C/ C = = K Excel Lagmur K C8D8 Graph2 26.54 438.65 D9 C9 D8 D8C8 C8D8 Graph2 26.54 438.65 D9 C9 D8 D8C8
Excel =/ =438.65, =/( K)=26.54 K =/D80.00228 molg -, K =/ /=/D8/C86.5 D9 C9 K Lagmur Lagmur Excel F5F30 C b [mol/l] [mol/g]graph C b [mol/l] C b [mol/l] [mol/g] Lagmur KC = + KC (3.0) K =/D80.00228 molg -, K =/ /=/D8/C86.5 D9 C9 K Graph G5G30 F5F30 C b [mol/l] [mol/g] G5 D9 C9 K $$D$9, $C$9 =$D$9*$C$9*F5/(+$C$9*F5) Lagmur G5 F5 D9 C9
G5 G5 G6G30 Sheet Graph Graph ( Freudlch BET ) Excel Excel Excel Excel C b [mol/l] [mol/g] C b / Excel C b [mol/l] [mol/g] C b [mol/l] C b /[g/l] 3.9 Freudlch C b Freudlch ab Freudlch Freudlch = ac b ab l = l a + l C b b l C b l /b l a C b ad_gra.xls b
l C b [-] =l(b5) B5 Cb l [-] =l(c5) C5 l C b [-]l [-] l C b [-]l [-]
y = 0.369x 5.93 l = 0.369 lcb 5.93-5.93 0.369 a/b 93 l a = 5.93 a = e 5. = 0.00270 = 0.369 b a Excel =exp(-5.93) Ferudlch a/b Ferudlch a/b Ferudlch Ferudlch = ac b a /b C b Ferudlch b Lagmur Excel Ferudlch = ac b b a/b 0.369 0.00270 b = C Excel
=0.0027*G5^0.369 G5 C b 0 C b 0.5 C b Freudlch Graph C b [mol/l] [mol/g] Lagmur 3.0 Freudlch ac /b () C a b (Lagmur)
KC = + KC (2) K Freudlch Lagmur 3. (a) Hery KC (3) K 3- (a) C C p 0 (a) 0 (b) 0 0 (c) (d)bet 3-
(b)lagmur 3-2 [-] r [mols - ] a[mols - ] 3-2 Lagmur ra (6) r[mols - ](-)[-] C[mol m -3 ] r b(-)c (7) b[m 3 s - ] ab(-)c (8) b C θ = (9) a + b C [molkg - ] b/a K[m 3 mol - ](K )θ =
KC = + KC (0) (c)(freudlch) 3-(c) ac /b () [molkg - ] a b [-] (d)bet(bruauer-emmett-teller) BET 3-(d) BET 3-3 q = q K C ( C) ( + KC C) 3-3 Lagmur 2 3- K K
3-, C, C/ C [molm -3 ] [molkg - ] C/ [kgm -3 ] 460 2.68 72 225.99 28 09.49 73.2 52.9.05 50.4 2.4 0.60 35.7 (3.0) K C = + KC (3.3) C C ( + KC ) C KC = K = C = C (3.4) = =( K)(3.4) C/ C = = K 3- C/ C 3-3-4 =/ =438.65, =/( K)=26.54 K =/D80.00228 molg -, K =/ /=/D8/C86.5 K = / [ molkg - ] Lagmur : C [ molm -3 ] 3-4 3-5 2 C
Freudlch C b C b [mol/l] [mol/g] log 0 C b log 0 0.462 0.00205 0.240 0.0075 0.39 0.0039 0.059 0.0005 0.0243 0.0008 Freudlch ab b = ac b () ab l = l( ac b b = l a + lc ) b b = l a + lc b b l C b l x=l C b y=l l = l a + lc b (3) b y=a 0 +a x a a 0 = l a( ) a = e = ( ) b a 0 a 0 a a = a 0 = a= b= =ac /b b c b =00.5 (2) (4)
BET BET KC = ( C)( C + KC) 7 BET KC ( C) = ( C + KC) 8 8 ( C) ( C + KC) = KC + ( K ) C = KC 9 9 C C ( C) C( + ( K ) C) = KC 20 20 C ( C) = K K + C K 2 C K C (- C) K K
3.2 Hb HcCbCa Hd Oa ObHa Ob Ca 2.9 Oa 3.6 [m 2 ] 3-6 Ca Oa.32 Ca Ob.206 OaCaOb 2.9 C 0.77 O 0.62 S 8 8 20 s' = ( 3.36 0 ) ( 3.99 0 ) = 6.37 0 m 2 2 S[m 2 /g] 23 S = 6.02 0 s' S [m 2 /g] [mol/g] s [m 2 ] 6.020 23 [/mol] S[m 2 /g] = 0.00335 mol/g 20 s ' = 6.37 0 m 2 23 20 S = 0.00335 6.02 0 6.37 0 = 28.5 m 2 /g
4. 4. 4.2 [] [] 25 0,20,40,50,60,80,00 wt% vs [] dt / w = (4.) d t w w : [g/cms] :8.0070-3 g/cms [g/cms]25 d : [g/cm ] d :0.99704g/cm 3 [g/cm ]25 t : [s] t : [s] W: dt = w (4.2) d w t w /d [cm /s] (4.3)
[] ) b 20m f 325 0 4 a b 5 4- bd 6 3 7 80m 463 7 4- [wt.%] d[g/cm 3 ] / w [-] 0 20 40 50 60 80 00
4.3 [] 4-2 w'g 25 0 42 w g 25 d g/cm 0.99704g/cm 3 V w w = (4.4) d w' w
V wg w w' d = (4.5) V d 4-2 25 w [g] w w [g] V[cm 3 ] w[g] d[g/cm 3 ] [wt.%] 0 20 40 50 60 80 00 0.99704 [wt.%] w [g] w w [g] V[cm 3 ] w[g] d[g/cm 3 ]
4.4 [] [ ] PVA; -CH 2 CHOH- 0.5g00cc 2/3, /2, /3, /5 l( / w ) [] C - w C w vs [] 4-3 [g/00cc] d[g/cm 3 ] / w [-] 0.5(/5) 0.5(/3) 0.5(/2) 0.5(2/3) 0.5
[] PVA M - w w l( / w ) C w =8.0070-3 g/cms [] M []KM (4.6) K=6.660-4 0.64 [] - [ ] = lm (4.7) w C 0 C w l( / w ) [ ] = lm (4.8) C C 0 - w l( / ) C C w C C w [] - w C w C 4.3 0.32420.0874 0.3242 l( / w ) C 4.4 0.32490.0256 0.3249 - w C w C 0.3242 PVA M 4.6 PVA M M=/K / 0.3242/6.660-4 /0.64 58
[] w c w - w 4.3 298K PVA C C w [] l( / w ) c l( / w ) 4.4 298K PVA C C
5. DNA 5. DNA 5.2 DNA DNA DNA TE ph 5.3 ) 30ml 4060 2) ) 3) 2) 4) 5) DNA DNA DNA DNA 6).5ml 70% 8 5) DNA 70% DNA DNA DNA TE ml DNA 5.4 DNA ) DNA DNA
A 0 H Buffer 2µ DNA 5 B Eco RI 0 H Buffer 2µ DNA 5 2 2) ) A B 20 00V DNA 3) 7 30 4) DNA + 5. 500bp 6 5 4 3 2 DNA 2 DNA 3 DNA 4 -DNA 5 -DNA 6 5.2 DNA 5.5 DNA DNA
DNA DNA DNA DNA (A,T,G,C) DNA DNA(dNTPs) (EtBr) (DNA) UV =302m EtBr () DNA 5.3 DNA 5.6 DNA 4 GATC DNA Eco RIGAATTC Eco RI GAATTC CTTAAG H diii AAGCTT TTCGAA 5.4 DNA. 2.
6. 0
() (S Fe) NH 3 Cl NH 2 NaOH + N 2 Cl O N C0H7ONa ( 2)
7. 7. GCMS GCMS 7. Gas ChromatographGC Mass Spectrometer MS 7. GABA GCMS GCMS 405MPa GCMS 7. 7.2 Web Wkpedacaffee.3.7-(C 8 H 0 N 4 O 2 ) () C 8 H 0 N 4 O 2 H 2 O,C 8 H 0 N 4 O 2 238 Wkpedahttp://www.wkpeda.org/ GCMShttp://www.a.shmadzu.co.jp/products/gcms/qp200.htm http://www.kagaku.com/jasco/39-49.html
GABA A4 GCMS A4 g 7.2 GCMS 7.3 7.4 7.4 GCMS
GCMS 6 GC7 MS 3 GCMS 5.6. 8.0. GCMS 7.5 7.6 7.5 GCMS 7.6 M.W.=94 7.5 2.6m GABA GCMS 7.5 7.6
7.3 GCMS GCMS 7.3 g 50cc 405MPa0 GCMS ON GCMS ON GCMS GCMS GCMS GCMS 7.3 ON GCMS ON GCMS GCMS OK OK 7.7 GCMD GCMS GC-MS
2 GCMS 7.8 GCMS 3GCMS 7.9 GCMS 4)
GC-MS 7.0 7. 0 7.0 RR
60 3060
(x y )(=2) y= a 0 + a x y y = a + bx ) ( ε = y y ( a 0 a ) ) f (x) = a 0 + a x (A-) (A-) y e (A-2) e = y f (x ) = y ( a 0 + a x ) (A-2) (A-2) S S = = e 2 = = 2 { y ( a + a x )} (A-3) 0 S a 0 a (A-3) a 0 a S a 0 = = a 0 2 { y ( a0 + ax )} = 2 { y ( a0 + ax )} (A-4) = a S a = 2 [{ y ( a + a x )} x ] (A-5) = 0 S S a 0 S = 0 = 0 a (A-6) a 0 a (A-6) = y = = a0 + a x (A-7) = = y x = = a0 x + a x (A-8) = 2 (A-7)(A-8) a 0 a
= = = = = = = x x x x y y x a 2 2 2 0 ) ( ) )( ( ) )( ( (A-9) = = = = = = x x y x x y a 2 2 ) ( ) )( ( ) ( (A-0) (A-9)(A-0) y = a 0 + a x y=a x+a 0 e x y y=a x+a 0 S S a 0 a y=a x+a 0 S=e ={y -(a x +a 0 )} S (S a 0 )=0 (S a )=0 2a 0,a S