PB-9 Proteins, Peptides & Nucleic acids http://www.ymc.co.jp Ion Ex change Chr omat atog ogra raph phy Size Exclusion Chr omat ography Re verseded Phase Chr om at ogra phy Normal Phase Chr hrom omat og raph y
MC iosep ro duc CONTENTS CO NTENTS 7
Column Selection Guide
Proteins, Peptides, Saccharides IgG Transferrin Albumin N8E min Eluent : A) mm Tris-HCl (ph 8.6) B) mm Tris-HCl (ph 8.6) containing. M NaCl -%B (- min), -%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 8 nm Injection : L ( L/mL) N866A 6 min Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl Flow rate :. ml/min Temperature : ambient ( C) Detection : UV at 8 nm Injection : L ( L/mL) IgG Albumin Transferrin P8A NaN Mouse monoclonal IgG (Anti-human IgG) 6 8 6 min P8A Mouse monoclonal IgG (Anti-human IgG) NaN min Eluent : A) mm Tris-HCl (ph 8.) B) mm Tris-HCl (ph 8.) containing. M NaCl - %B (-8 min) Flow rate :. ml/min Temperature : C Detection : UV at nm Injection : L (. mg/ml) Eluent :. M KHPO-KHPO (ph 7.) Flow rate :.7 ml/min Temperature : ambient ( C) Detection : UV at nm Injection : L (. mg/ml). Rabbit IgG. Mouse IgG Fc fragment Mouse IgG Fc fragment R86D min Eluent :. M KHPO-KHPO (ph 6.9) containing. M NaCl Flow rate :. ml/min Temperature : ambient (7 C) Detection : UV at nm Injection : L (. mg/ml) R869B min Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -%B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm Injection : L (. mg/ml) YMC Bioseparation Products
N8B 6 min 6 N86L * 6 min N88B 6 min Eluent : A) mm Tris-HCl (ph 8.6) B) mm Tris-HCl (ph 8.6) containing. M NaCl -%B (- min), -6%B (-6 min) Flow rate :. ml/min Temperature : C Detection : UV at nm Injection : L Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -%B (- min), -%B (- min), %B (-6 min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm Injection : L * undigested BSA * * MW. Myoglobin 7,). Insulin (Bovine),7). Neurotensin,67). Tetraglycine 6). Glycine ) Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl/acetonitrile (7/) Flow rate :.7 ml/min Temperature : ambient ( C) Detection Injection : UV at nm : L min min G967A G96A Eluent : methanol/ mm NHHPO (/9) Flow rate :. ml/min Temperature : 7 C Detection : FLS at Ex. nm, Em. nm Injection : L (. pmol/ml) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. Eluent : methanol/ mm NHHPO (8/) Flow rate :. ml/min Temperature : 7 C Detection : FLS at Ex. nm, Em. nm Injection : L (. pmol/ml) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD.
Nucleic acids N8G min Eluent : A) mm Tris-HCl (ph 8.) containing.7 M NaCl B) mm Tris-HCl (ph 8.) containing. M NaCl -%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 6 nm Injection : L Plasmid pbr Hae digest (8-87 bp) Plasmid pbr (,6 bp) N86F min Eluent : A) mm Tris-HCl (ph 8.) B) mm Tris-HCl (ph 8.) containing. M NaCl 7-8%B (- min), 8%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 6 nm Injection : L Plasmid pbr Hae digest (8-87 bp) Plasmid pbr (,6 bp) P867A min Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl Flow rate :.7 ml/min Temperature : ambient ( C) Detection : UV at 6 nm Injection : L '-CCGCTCGAGCTAAAAAAAGCCTGTGTTACC-' ( mer) 7 8 9 mer F6C- min UV TIC Eluent : A) mm DBAA* (ph 6.) B) mm DBAA* (ph 6.)/acetonitrile (/) 8-6%B (- min), 6%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 69 nm and ESI negative-mode Injection : L ( pmol/component) * di-n-butylammonium acetate YMC Bioseparation Products
Ion Exchange Chromatography YMC-BioPro series AU.. N() N() N() Rs(,) Rs(,) 7 76 7.6 6.8.8-. MPa. N7K min AU... N7J N() N() N() Rs(,) Rs(,) min 6...9-. MPa. Ribonuclease A (. mg/ml). Cytochrome c (. mg/ml). Lysozyme (. mg/ml) Eluent Flow rate Temperature Detection Injection : A) mm KHPO-KHPO (ph 6.8) B) mm KHPO-KHPO (ph 6.8) containing. M NaCl - %B (- min) for X mmi.d. - %B (-7 min) for X mmi.d. :. ml/min : C : UV at nm : L 7 YMC Bioseparation Products
AU. 6 min AU. 6 min AU. 6 min IgG Transferrin N76E N78F N78D Albumin N8E min. Ribonuclease A (. mg/ml). Cytochrome c (. mg/ml). Lysozyme (. mg/ml) Eluent : A) mm KHPO-KHPO (ph 6.8) B) mm KHPO-KHPO (ph 6.8) containing. M NaCl -%B (-6 min) Flow rate :. ml/min for mmi.d..9 ml/min for. mmi.d. Temperature: C Detection : UV at nm Injection : L for mmi.d..6 L for. mmi.d. peaks = N8B 6 min N8A min IgG Transferrin IgG Transferrin Albumin Albumin peaks = 8 N87C 6 min peaks = N8G min Eluent Flow rate Temperature Detection Injection Sample : A) mm Tris-HCl (ph 8.6) B) mm Tris-HCl (ph 8.6) containing. M NaCl -%B (- min), -%B (- min) :. ml/min : C : UV at 8 nm : L : Human serum ( L/mL) N87G 6 min Eluent Flow rate Temperature Detection Injection Sample : A) mm Tris-HCl (ph 8.6) B) mm Tris-HCl (ph 8.6) containing. M NaCl -%B (- min), -6%B (-6 min) :. ml/min : C : UV at nm : L : Tryptic digest of BSA 8
Ion Exchange Chromatography YMC-BioPro series AU.. Column : YMC-BioPro QA X mmi.d. A (porous Q type) X mmi.d. B (porous Q type) X. mmi.d. Linear velocity :. cm/min Sample : mg/ml Bovine serum albumin (BSA) in equilibration buffer Equilibration buffer : mm Tris-HCl (ph 8.6) Elution buffer : mm Tris-HCl (ph 8.6) containing. M NaCl Detection : UV at 8 nm BioPro QA % breakthrough 6 8 6. Ovalbumin. Trypsin inhibitor N86B N87C N87B N86E N88C N88B N86A N86D min min 7 7 7 7 7 Bioseparation YMC Products.... Eluent : A) mm Tris-HCl (ph 8.) B) mm Tris-HCl (ph 8.) containing. M NaCl -8%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 8 nm Injection : L 9
AU... AU. N7K N76E 6 min P8A N8G N8B. ml/min.8-. MPa min. ml/min.- MPa. Ribonuclease A. Cytochrome c. Lysozyme Eluent Temperature Detection Injection min P8B NaN NaN MAb MAb min : A) mm KHPO-KHPO (ph 6.8) B) mm KHPO-KHPO (ph 6.8) containing. M NaCl - %B (- min) for YMC-BioPro SP-F -%B (-6 min) for YMC-BioPro SP : C : UV at nm : L X mmi.d. min X mmi.d. Eluent : A) mm Tris-HCl (ph 8.) B) mm Tris-HCl (ph 8.) containing. M NaCl -%B (-6 min) Flow rate :. ml/min Temperature : C Detection : UV at nm Injection : L (. mg/ml) Sample : Mouse monoclonal IgG anti-human IgG (Purified by DEAE chromatography, containing NaN) min Eluent : A) mm Tris-HCl (ph 8.) containing.7 M NaCl B) mm Tris-HCl (ph 8.) containing. M NaCl : -%B(- min) Flow rate :. ml/min Temperature : C Detection : UV at 6 nm Injection : L (. mg/ml)
Size Exclusion Chromatography YMC-Pack Diol MW 6 Diol- 6 Diol- 7 8 9 Diol-. IgM. Thyroglobulin. IgA. Fibrinogen. -Globulin 6. IgG 7. Transferrin 8. HSA (human serum albumin) 9. -Antitrypsin. Ovalbumin. Carbonic anhydrase. Trypsin inhibitor. Myoglobin. -Lactalbumin. Ribonuclease A 6. Cytochrome c MW 9, 67, 9,, 8,,, 66,,,,, 7,,,7, 6 7 9 Elution volume (ml) Column : YMC-Pack Diol X 8. mmi.d. Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl Flow rate :. ml/min Temperature : C Detection : UV at 8 nm. Glutamate dehydrogenase. Lactate dehydrogenase. Enolase. Adenylate kinase. Cytochrome c MW 9,, 67,,, Column : YMC-Pack Diol X 8. mmi.d. Eluent :. M KHPO-KHPOpH 7. containing. M NaCl Flow rate :.7 ml/min Temperature : ambient Detection : UV at 8 nm min min min YMC Bioseparation Products
N866A N866E N866M N867B N866F N866B N866C N866D N866K N866G N866H 6 min Eluent :. M KHPO-KHPO (ph 7.) containing. M NaCl Flow rate :. ml/min Temperature : ambient ( C) Detection : UV at 8 nm 6 P88A Albumin Transferrin IgG 6 min Eluent :. M KHPO-KHPO (ph 7.) Flow rate :.7 ml/min Temperature : ambient ( C) Detection : UV at nm Injection : L Sample : Mouse ascites fluid (6 times dilution with water) min. Insulin (Bovine). Neurotensin. Angiotensin. Glycine Column : YMC-Pack Diol-6 X 8. mmi.d. Eluent :. M KHPO-KHPO ph 7. containing. M NaCl/ acetonitrile7/ Flow rate :.7 ml/min Temperature : ambient Detection : UV at nm MW 7 6 8 9 6 7. Myoglobin. Ribonuclease A. Cytochrome c. InsulinBovine). Insulin B chain 6. -Mating factor 7. Neurotensin 8. Angiotensin 9. CCK-Octapeptide. Bradykinin. Angiotensin. Oxytocin. Angiotensin. Met-Enkephalin. Leu-Enkephalin 6. Tetraglycine 7. Glycine MW 7,,7,,7,96,68,67,96,,6,,7 9 7 6. P-8. P-. P-. P- MW 8, 8,,7,8 MW 6 6 7 8 Diol-6 Diol- Diol- 9 Diol- Elution volume (ml) MW. Pullulan (P-8) 8,. Pullulan (P-) 8,. Pullulan (P-) 86,. Pullulan (P-),. Pullulan (P-) 8, 6. Pullulan (P-),7 7. Pullulan (P-), 8. Pullulan (P-),8 9. Maltopentadecaose (G),8. Maltoundecaose (G),8. Maltoheptaose (G7),. Maltopentaose (G) 8. Maltotriose (G). Maltose (G). Glucose (G) 8 min Elution volume (ml) Column : YMC-Pack Diol, X 8. mmi.d. Eluent : water Flow rate :. ml/min Temperature : ambient Detection : RI
Reversed Phase Chromatography r 6 min 7 A8B 6 min. BAM-P. [D-Ala,Met ]-Enkephalinamide. -Endorphin. Met-Enkephalin. [D-Ala,Met ]-Enkephalin 6. -Endorphin 7. -Endorphin 7 AD,) 87),76) 7) 88),899),6) Eluent : A) water/tfa(/.) B) acetonitrile/tfa (/.) -%B (- min), %B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm YMC Bioseparation Products
8 6 PC=8 * NG 8 6 6 8 PC= * ND 6 8 min min Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -%B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm Injection : L Sample : Tryptic digest of -Lactoglobulin B C8, nm C8, nm 6 76.D, 6 79.D C8, nm C, nm 6 6 8.D min 7.D min C8, nm. Cytochrome c. Insulin (Bovine). Amyloid -protein. Lysozyme. -Lactalbumin 6. Myoglobin,),7),),),) 7,) Column : m, X mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -6%B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm 6 8.D min
Reversed Phase Chromatography r A) water/tfa (/.) B) acetonitrile/tfa (/.).D 97.D min min. BSA 66,). Conalbumin 77,). Lipoxidase 96,) Column : YMC-Pack C ( nm) m X mmi.d. Eluent : -%B (- min), %B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm A) water/tfa (/.) B) acetonitrile/-propanol/tfa (//.) C, nm. BSA 66,). Conalbumin 77,). Lipoxidase 96,) Column : m, X mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/-propanol/tfa (//.) -%B (- min), %B (- min) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm 97.D 8 6 min C, nm C8, nm 7.D 78.D C, nm C8, nm 7.D 8.D 8 6 min 8 6 min YMC Bioseparation Products
S999A min Column : Hydrosphere C8 m X mmi.d. Eluent : mm KHPO-KHPO (ph 6.9) Flow rate :. ml/min Temperature : 7 C Detection : UV at nm Injection : 8 L (.. mg/ml) min A9A Column : Hydrosphere C8 m X mmi.d. Eluent : mm KHPO-KHPO (ph.) Flow rate :. ml/min Temperature : C Detection : UV at 6 nm Injection : L (.. mg/ml) T T T J9A T T T T JA min T- T Impurities T mer Recovery 6% JE J9B min. 7. min Impurities mer min 7.. min Recovery % Column : m, X mmi.d. Eluent : A) mm or mm TEAA* (ph6.) B) mm or mm TEAA* (ph6.)/acetonitrile (8/) -6%B (- min) Flow rate :. ml/min Temperature : C Detection : UV at 69 nm * triethylammonium acetate '-CCGCTCGAGCTAAAA AAAGCCTGTGTTACC-' purity>99% Eluent : A) mm DBAA* (ph6.)/methanol (6/) B) mm DBAA* (ph6. )/methanol (/8) -%B (- min) Temperature : ambient Detection : UV at 69 nm Sample : synthetic oligonucleotide ( M) * di-n-butylammonium acetate 6
Reversed Phase Chromatography YMC-Pack PROTEIN-RP 9 8 7 6 Eluent : water/tfa (/.) Flow rate :. ml/min Temperature : ambient Column : X mmi.d. Eluent : acetonitrile/water (/6) Flow rate :. ml/min Temperature : C Detection : UV at nm. Uracil. Benzene. Naphthalene. Diphenyl min N() min N() 8 8 7 6 9. Met-Enkephalin. Leu-Enkephalin. Oxytocin. Bradykinin. Angiotensin I 6. Ribonuclease A 7. -Mating factor 8. Insulin (Bovine) 9. Cytochrome c. Lysozyme. BSA. -Lactoglobulin. Ovalbumin 7) ),7),6),96),7),68),7),),) 66,) 8,),). Cytochrome c. BSA. Ferritin,) 66,),) Column : YMC-Pack PROTEIN-RP m X mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -9%B (-6 min) Flow rate :. ml/min Temperature : ambient min min Detection : UV at nm Column : YMC-Pack PROTEIN-RP m X mmi.d. Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -9%B (- min) Flow rate :. ml/min Temperature : ambient Detection : UV at 8 nm 7 YMC Bioseparation Products
Normal Phase Chromatography 6 min A99E, 6 8 min A997I. TRP-GLY. GLY-GLY-PHE. Angiotensin I. Substance P. Insulin (Bovine) 6) 79),96),8),7) Eluent : A) acetonitrile/tfa/tea (/./.) B) water/tfa/tea (/./.) -%B (- min), %B (- min) Flow rate :. ml/min Temperature : ambient Detection : UV at nm Eluent : A) water/tfa (/.) B) acetonitrile/tfa (/.) -%B (- min), %B (- min) Flow rate :. ml/min Temperature : ambient Detection : UV at nm 6 6... OH Phenol OH OH Catechol OH HO Resorcinol HO OH OH Hydroquinone OH HO Pyrogallol OH HO OH Phloroglucinol Eluent : hexane/ethanol (8/) Flow rate :. ml/min Temperature : C Detection : UV at nm.. 6. min A6A min A6C 8
Normal Phase Chromatography YMC-Pack Polyamine 9 Column : YMC-Pack Polyamine II X mmi.d. Eluent : acetonitrile/water (/) Flow rate :. ml/min Temperature : C Detection : RI, -6 RIU/FS Injection : L (-7 mg/ml) min F68H 8 6 YMC Bioseparation Products 6 6 Raffinose Lactitol Palatinit Lactose Maltose Maltitol Inositol Palatinose Sucrose Galactose() Mannose() Arabitol Ribitol Xylitol % 8% 8% Glucose Mannitol Sorbitol Fructose Xylose Fucose Glycerol Column : YMC-Pack Polyamine II X mmi.d. Temperature : C Erythritol Rhamnose Sucralose
Packing Materials YMCGEL Eluent Temperature Detection Purity: 9.9% : A) water/tfa (/.) B) acetonitrile/tfa (/.) 7%B (- min), 7-%B (- min) : ambient : UV at nm min min min Sample : Insulin bovine / Insulin human = / (w/w) Pro C8 ODS-A ODS-AQ PolymerC8 C8 Load: mg Purity: 99.% ASS ASS6 AAS AAS AAS AAS6 AAS6 AAS6 AAS AAS AAS AAS AAS AASA AQS AQS AQS6 AQS6 AQS AQS PC99S OCS OCS OCS OCS6 OCS6 OCS6 OCS OCS OCS Ph PHS PHS6 PHS C BUS BUS BUS BUS6 BUS6 BUS6 BUS BUS BUS CN CNS CNS6 CNS YMCbasic BA99S Diol DLS DLS6 DLS6 PROTEIN-RP PR99S
QA SP QA-F SP-F Non-porous Non-porous QAAS-6WP SPAS-6WP QFS-6WP QFS-6WP QFS-6WP SFS-6WP SFS-6WP SFS-6WP,,,, 8,,, 8, 8. 8. 8. 8. 8. 8. 8. 8. Diol-6 Diol- Diol- Diol- 6 DL6S-6WT DL6S-8WT DL6S-8WT DL6S-WT DL6S-WT DLS-6WT DLS-8WT DLS-8WT DLS-WT DLS-WT DLS-6WT DLS-8WT DLS-8WT DLS-WT DLS-WT DLS-6WT DLS-8WT DLS-8WT DLS-WT DLS-WT 9,, 6,, 6, 9,, 6,, 6, 9,, 6,, 6, 9,, 6,, 6,......... 6. 6. 6.......... S- (X)SS-HWT (X)SS-WT (X)SS-WT (X)SS-HPWT (X)SPWT (X)SS-PWT (X)SS-HWT (X)SS-WT (X)SS-LWT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-H6WT (X)S6WT (X)SS-L6WT (X)SS-6WT (X)S6WT (X)SS-6WT (X)SS-L6WT (X)SS-6WT (X)SS-WT (X)SS-WT (X)SS-PWT (X)SS-PWT (X)SS-HWT (X)SS-WT (X)SS-LWT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-LWT (X)SS-WT (X)SS-WT (X)SS-WT,, 6,,, 6,,,, 6,,,, 8, 8,,,, 8, 8,,, 6,, 6, 7,,,, 6,, 6,,,, 8, 6, 6. 6. (X)SS-6WT (X)SS-L6WT (X)SS-6WT (X)SS-6WT (X)SS-6WT (X)SS-6WT (X)SS-6WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT (X)SS-WT,,, 8, 6, 6, 76, 96,, 8,, 8,......... 6. 6. 6.......... 6. 6. S- 8 8 RS8S-HWT RS8S-WT RS8S-WT RS8S-HPWT RS8PWT RS8S-PWT RS8S-HWT RS8S-WT RS8S-LWT RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-H6WT RS86WT RS8S-L6WT RS8S-6WT RS86WT RS8S-6WT RS8S-L6WT RS8S-6WT RS8S-WT RS8S-WT RS8S-PWT RS8S-PWT RS8S-HWT RS8S-WT RS8S-LWT RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-LWT RS8S-WT RS8S-WT RS8S-WT RS8S-6WT RS8S-L6WT RS8S-6WT RS8S-6WT RS8S-6WT RS8S-6WT RS8S-6WT RS8S-WT RS8S-WT RS8S-WT RS8S-WT RS8S-WT,, 6,,, 6,,,, 6,,,, 8, 8,,,, 8, 8,,, 6,, 6, 7,,,, 6,, 6,,,, 8, 6,,,, 8, 6, 6, 76, 96,, 8,, 8, Ordering Information YMC Bioseparation Products
... 6. 6....... 6. 6. 6. 6..... 6. 6. 6. 6. S- AAS-HWT AAS-WT AAS-LWT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-L6WT AAS-6WT AA6WT AAS-6WT AAS-6WT AAS-WT AAS-WT AAS-PWT AAS-PWT AAS-LWT AAS-WT AAS-WT AAS-WT AAS-WT AAS-6WT AAS-L6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-6WT AAS-6WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-PWT AAS-PWT AAS-LWT AAS-WT AAS-WT AAS-L6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-6WT AAS-WT AAS-WT AAS-WT AAS-WT AAS-WT 8,,,, 8,,,,,,,,, 6,, 6, 7,,, 6,, 6,,,,, 6, 6,,, 7,, 8,,,, 8,, 6, 8,, 8, 6,, 6, 7,,, 6,, 6, 6, 8, 9, 6, 6, 8, 9,,, 68,, 8,... 6. 6....... 6. 6. 6. 6. S- AQS-HWT AQS-WT AQS-LWT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-6WT AQ6WT AQS-6WT AQS-6WT AQS-WT AQS-WT AQS-PWT AQS-PWT AQS-LWT AQS-WT AQS-WT AQS-WT AQS-WT AQS-L6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-6WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-WT AQS-6WT AQS-6WT AQS-WT AQS-WT,, 6,,,,,,,,, 7, 6,, 6, 7,,, 6,,, 8,,,, 78,, 7, 8, 8, 96,,,, 8,, 6, 8,, 8,....... S- (XX)S-HWT (XX)S-WT (XX)S-LWT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-6WT (XX)6WT (XX)S-WT (XX)S-WT (XX)S-PWT (XX)S-PWT (XX)S-WT (XX)S-WT (XX)S-L6WT (XX)S-6WT (XX)S-6WT (XX)S-6WT (XX)S-6WT,, 6,,,,,,,, 6,, 6, 7,, 6, 8,,,, 78,
URL http://www.ymc.co.jp 6-86 8 YMC F TEL.-FAX.- ISO 9 JQA-7 88K - -6- F TEL.687-FAX.687-9 6. 6. 6. 6..... 6. 6. 6. 6. (XX)S-6WT (XX)S-6WT (XX)S-6WT (XX)S-6WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (XX)S-WT (ZZ)S-6WT (ZZ)S-6WT (ZZ)S-WT (ZZ)S-WT (YY)S-WT (YY)S-WT (YY)S-PWT (YY)S-PWT (YY)S-WT (YY)S-WT (YY)S-L6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-6WT (YY)S-WT (YY)S-WT (YY)S-WT (YY)S-WT (YY)S-WT, 7, 8, 8, 96,,, 8,, 8, 6, 8,,, 6,, 6, 7,, 6,, 6, 6, 8, 9, 6, 6, 8, 9,,, 68,,,.... 6. 6. S- BA99S-HWT BA99S-WT BA99S-LWT BA99S-WT BA99S-WT BA99S-WT BA99S-WT BA99S-WT BA996WT BA99S-6WT BA996WT BA99S-WT BA99S-WT BA99S-6WT BA99S-6WT BA99S-6WT BA99S-6WT BA99S-6WT BA99S-6WT 8,, 8, 67, 78, 8, 6,, 8, 6,, 7, 7, 8, 6, 7, 78, 7, 86, PR99S-WT PR99S-WT PR99S-6WT PR99S-6WT PR99S-WT PR99S-WT PR99S-WT 8, 9, 6, 8,,,, 6. 6. S-6 PC99S6-LWT PC99S6-WT PC99S6-6WT PC99S6-6WT PC99S6-6WT PC99S6-6WT PC99S6-WT, 6,, 6, 6, 6, 8, 6 DN6S-6WT DN6S-6WT DN6S-6WT DN6S-WT DN6S-WT DNS-6WT DNS-6WT DNS-6WT DNS-6WT DNS-WT DNS-WT 6, 7, 9,,,, 6, 7, 9,,, 6. 6. PBS-6WT PBS-6WT PBS-6WT PBS-6WT PBS-WT PBS-WT, 6,, 8, 6,,