OECD GLP OECD OECD 1960 30 OECD TG 2001 10 17 8 TG 202 208 TG 201 1984 6 TG 202 1984 4 TG 202 2000 10 TG 203 1992 7 TG 204 14 1984 4 TG 205 1984 4 TG 206 1984 4 TG 207 1984 4 TG 208 1984 4 2000 TG 209 1984 4 TG 210 1992 7 TG 211 1998 9 TG 212 1998 9 TG 213 1998 9 TG 214 1998 9 TG 215 1998 9 TG 216 1998 9 TG 217 1998 9 TG 218 2001 2 TG 219 2001 2 TG 220 2000 3 TG 221 2000 10 TG 2000 4 TG 2000 1 1
TG 201 Selenastrum capricornutum 1 TG 201 Selenastrum capricornutum Scenedesmus subspicatus Chlorella vulgaris 72 5 ( 2 ) 10 4 cells/ml(selenastrum capricornutum Scenedesmus subspicatus) 100mg/L 21 25 2 400 700nm 0.72 10 20 photons/m 2 s ( 24 48 72 ) ( ) ph( ) EC50 NOEC TG 202 TG 211 TG 202 1984 1998 TG 211 TG 202 2
TG 202 TG 211 TG 202 1 Daphnia magna 2 21 3 40 10 4 4 2 TG 202 Daphnia magna 24 ph 6 9 NaCl, NaOH 140 250mg/L(CaCO 3 ) 48 1.5 2.2 3.2 4 20 / 100mg/L 18 22 1 16 8 24 48 DO H( ) 24 48 50 EC50 3
TG 211 Daphnia magna 24 ( 1 3 ) 21 ( F1 3 14 21 ) 3.2 5 18 22 2 10 / 40 / i.e. 0.1ml/L 0.1ml/L 16 8 15 20 E/m 2 s ( ) DO ph 1 NOEC 1, 2, 4, 7, 14 EC 50 ( ) TG 203 TG 204 TG 210 TG 212 TG 215 TG 204 TG 203 TG 210 LOEC (Lowest Observed Effect Concentration ) NOEC (No Observed Effect Concentration ) TG 212 TG 210 TG 210 4
TG 203 96 ( 2.2) 5 7 / 100mg/L 1.0g/L 12 16 / 24 3 / (24 (3 6 )) DO ph(1 / ) (24 ) 96 50 (LC50) 0 100 TG 204 14 1 2 NOEC 10 100mg/L 1.0g/L 12 16 / ( ) 1 / ( ( 2%)) (1 / ) ph DO (2 / ) NOEC 5
TG 210 3.2 5 60 / 2 0.1mL/L ( ) LOEC NOEC TG 212 3.2 5 30 / 3 0.1mL/L ( ) DO( 1 / ( )) ph( ( ) 1 / ( )) (1 ) ( ) LOEC NOEC 6
TG 215 28 5 0.1mL/L 7 / 12 16 / 2 4% DO( ph( EC 50 LOEC NOEC 4 OECD Earthworm TG 207 TG 220 TG 207 2 Chironomid BBA (1995) 1 TG 218 TG 219 2 1998 2001 1 BBA(1995): Long-term toxicity test with Chironomus riparius: Development and validation of a new test system. Edited by M. Streloke and H. Köpp. Berlin. 7
2 TG 218 TG 219 EC50 LOEC NOEC 10 Lemna sp. ASTM 1991 2 2000 10 TG 221 7 EC50 LOEC NOEC 11 TG 205 TG 206 TG 208 TG 209 TG 213 TG 214 TG 216 TG 217 12 16 2 ASTM American Society for Testing and Materials.(1991): Standards Guide for Conducting Statis Toxicity Test With Lemna gibba G3. E1415-91:10 pp. 8
TG 207 TG 207) TG 220 Eisenia foetida Eisenia foetida (Enchytraeus albidus) (Eisenia fetida fetida (Michaelsen ) (Michaelsen ) Enchytraeus Eisenia fetida andrei) 10 10 20 0.3-1.0 20 0.3-1.0 750g( ) ph 6.0 0.5 ph 6.0 0.5 69 ] 70 ] 48 72 14 21 4 NOEC 5 ( NOEC 5 ( 1.8 ) 1.8 ) ECx 12 ( 2 ) ECx 12 ( 2 ) 5 5 NOEC ECx 8 ( NOEC ECx 8 1.8 ) ( 1.8 ) 1,000mg/L ( ( 1 / 10 20 10 / 4 10 / 0.20-0.25L 10 / 1-2L 6cm 200cm 2 500-600g 20 2 20 2 20 2 20 2 (400 800 ) 16 400 800 8 16 400 800 8 28 21 ( 7 14 ( 7 (21 (28 (6 ) (8 LC 50 LC 50 NOEC ECx NOEC ECx x 9
10 TG 218 TG 219 Chironomus riparius C. yoshimatsu C. tentans C. sp. 1 20-28 C. riparius C. yoshimatsu 28-65 C. tentans 5 3 Ecx4 NOEC 10g 10 / 20 2 C. riparius C. yoshimatsu 23 2 C. tentans 16 500 1,000 3 / 10 EC 50 LOEC NOEC 11 TG 221 Lemna gibba Lemna minor 7 ( 3.2 ) 5 100mg/L 24 2 400 700nm 85 125 E/m 2 s 6,500 10,000 ( 3 ) ( ) ph( ) (Td) ECx LOEC NOEC 10
12 TG 205 TG 206 1 1 2 20 3 1 1 1 6 8 21 ( 17-18 10 14 24-25 14 3 1000ppm 5 2 3 1 / 1 1 2 / 12 10 / 1 / 1 3 / 8 2 2 22 32 1 35-38 2 30-35 22 5 25 32 18 22 3-4 23-27 4 16-27 40-80 8 7 8 / 16 17 10 16 18 / 7 8 1 / 1 2 1 1 2 1 0, 5, 8 8 14 1 2 0 5 5 8 8 1 2 2 4 14 14 LC 50 LC50 5000ppm NOEC NOEC NOEC mg/kg / mg/kg / 11
13 TG 208,1984 4 1 3 1 3 0.5 1.5 20 m) 10 20 PH 5.0 7.5 50 14 1 3 4 5 50 2 70 LC50 EC50 1 ryegrass rice oat wheat sorghum 2 mustard rape Lolium perenne Oryza sativa Avena sativa Triticum aestivm Sorghum bicolor Brassica alba Brassica napus radish Raphanus sativus turnip Brassica rapa Chinese cabbage Brassica camperstris var. chinensis 3 vetch Vicia sativa mung bean Phaseolus aureus red clover Trifolium pratense fenugreek lettuce cress Trifolium ornithopodioides Lactuca sativa Lepidium sativum 12
14 TG 209 30 3 5 3.2 4g/L( 10 0 1 30 3 EC 50 95 1 16g 11g NaCl 3g l0.7g CaCl 2 2H 2 O 0.4g MgSO 4 7H 2 O 0.2g K 2 HPO 4 2.8g 1 13
15 TG 213 TG 214 TG 213 TG 214 50 50 100 200 1 L 48 24 10 48 24 10 96 96 10 10 2.2 5 2.2 5 10 10 3 95 1 25 2 50 70 25 2 50 70 500g/L 500g/L 4 24 24 4 24 24 LD 50 95 LD 50 95 50 95 50 95 14
16 TG 216 TG 217 TG 216 TG 217 28 28 25% 25% 100 100 2 PEC 5 2 PEC 5 5 5 0.1-0.1-0.5mm 20 2 20 2 0, 7, 14, 28 28 14 0, 7, 14, 28 28 14 0 28 0 28 7 7 28 28 25 25 EC 50, EC 25 EC 10 EC 50, EC 25 EC 10 15
5 OECD GUIDELINES FOR THE TESTING OF CHEMICALS http://www1.oecd.org/ehs/test/biotic.htm OECD 1999 Environmental Health &Safty News, No.9. 1998, ( ) 1985 OECD, 2000 16
OECD GLP GLP Good Laboratory Practice FDA OECD OECD 1979 OECD GLP OECD OECD-GLP 1981 OECD MAD Mutual Acceptance of Data: OECD OECD-GLP GLP 1983 OECD GLP 1984 GLP 1997 OECD-GLP 2000 12 OECD-GLP GLP OECD 1997 11 OECD-GLP GLP 16 17
GLP GLP GLP GLP GLP SOP GLP GLP GLP 16 18
GLP SOP SOP GLP SOP GLP GLP GLP 10 16 19