PCR 2013 4
Contents PCR...3 PCR...3...4 TaqMan...6 TaqMan / PCR / PCR...8 PCR...9...10 RNA...10 Cells-to-CT Kit...12 Cell-to-CT Kit...14 1 RT-PCR...15...16 TaqMan Fast...18 TaqMan...19 SYBR Green...22 TaqMan...24 TaqMan 96...25 TaqMan 384...28 TaqMan...29 RNAi...32 sirna...33 RNA sirna mirna...36 microrna...37 microrna...38 microrna...39 microrna...40 Non-coding RNA...42 microrna...43 SNP...44 SNP...45 SNP...46...48...49 High Resolution Melt HRM...50...52...55...56 TaqMan...59...60 INDEX...63 INDEX...69
PCR PCR TaqMan SYBR Green RNA PureLink RNA Kit TRIzol Reagent TRIzol Plus RNA Purification System TaqMan Cells-to-CT Kit Ambion Single Cell-to-CT Kit 10 10 10 13 14 MagMax RNAqueous Kit SYBR Green Cells-to-CT Kit 11 11 13 Super Script VILO 16 High Capacity RNA-to-cDNA Kit 17 1 RT-PCR TaqMan RNA-to-CT 1-Step Kit TaqMan Fast Virus 1-Step Master Mix 15 21 Power SYBR RNA-to-CT 1-Step Kit 15 PCR TaqMan Fast Advance Master Mix TaqMan Fast Universal PCR Master Mix TaqMan Universal Master Mix II TaqMan Gene Expression Master Mix 18 18 19 20 Power SYBR Green PCR Master Mix Fast SYBR Green Master Mix SYBR Select Master Mix 22 22 23 TaqMan TaqMan Gene Expression Assays TaqMan Array Plates TaqMan Array Gene Signature 96-well Plate TaqMan Gene Signature Arrays TaqMan Custom Arrays 24 25 26 28 28 RNAi sirna Silencer Select sirna TaqMan sirna Assays Ambion In Vivo sirna 33 35 35 Lipofectamie RNAiMAX Invivofectamine 2.0 36 36 microrna mirvana mirna Isolation Kit mirvana PARIS Kit TaqMan mirna ABC Purification Kit 38 38 38 microrna TaqMan MicroRNA RT Kit 39 Megaplex Primer Pools 39 PCR TaqMan Universal Master Mix II 19 TaqMan TaqMan MicroRNA Assays TaqMan Array MicroRNA Card TaqMan Pri-miRNA Assays TaqMan Non-coding RNA Assays 40 41 42 42 microrna microrna mimic & inhibitor mirvana mirna mimic & inhibitors 43 RNAiMAX Lipofectamine Invivofectamine 2.0 36 36 SNP TaqMan Genotyping Master Mix 46 PCR TaqMan GTXpress Master Mix 46 TaqMan TaqMan SNP Genotyping Assays 45 1 SNP TaqMan Sample-to-SNP Kit 47 TaqMan TaqMan Mutation Detection Assays 48 TaqMan TaqMan Copy Number Assays 49 HRM PCR MeltDoctor HRM 50 TaqMan TaqMan Protein Expression Core Reagents Kit with Master Mix TaqMan Protein Assay Kit TaqMan Protein Assay Open Kit 53 53 53 Protein Thermal Shift Dye Kit 55 Protein Thermal Shift Starter Kit 55 3
PCR TaqMan SYBR Green PCR TaqMan 5'- TaqMan TaqMan PCR TaqMan PCR5 - TaqMan PCR 5'- TaqMan TaqMan PCR DNA 5'-3' TaqMan 1.Polymerization TaqMan R Q 5' 3' 130 24 TaqMan Gene Expression Assays 2 2 2.Strand displacement TaqMan 3.Cleavage DNA 5'- 3' TaqMan 5' 4.Polymerization completed PCR 1. TaqMan 4
SYBR Green SYBR Green SYBR Green2 DNA SYBR Green I PCR 2 DNA PCRSYBR Green I PCR Tm PCR 2 DNA SYBR Green I Dye DNA SYBR Green AmpliTaq Gold DNA Polymerase 1.Reaction setup SYBR Green I Dye 2 DNA 2.Denaturation DNA SYBR Green I Dye DNA 3.Polymerization PCR 4.Polymerization completed SYBR Green I Dye 2 DNA PCR 2. SYBR Green 1. TaqMan SYBR Green TaqMan SYBR Green TaqMan 2 DNA SYBR Green I 3 PCR 2 TaqMan 2 PCR 2 1 2 PCR TaqMan Gene Expression Assays 130 1 3 2 PCR 5
TaqMan TaqMan TaqMan microrna mirna Small RNASNP TaqMan SNP TaqMan PCR TaqMan TaqMan Gene Expression Assay 23 130 / FAM VIC 4 2,700 TaqMan Drug Metabolism Genotyping Assay PharmaADME DME mirna non-coding RNA mirna non-codingrna ncrna mirna pri-mirna small RNA mirna ncrna TaqMan Protein Assay SNP TaqMan SNP 450 TaqMan SNP Genotyping Assay Custom TaqMan SNP Genotyping Assay TaqMan Copy Number Assay 160 18 Custom Plus TaqMan Copy Number Assays Custom TaqMan Copy Number Assays 2. TaqMan TaqMan Gene Expression 1 TaqMan MGB 24 Assays cdna Custom TaqMan Gene TaqMan Gene Expression Assays 24 Expression Assays TaqMan Array Plates TaqMan Gene Expression Assays 96 5 Fast 25 TaqMan Array Gene Signature 96-Well Plate TaqMan Gene Signature Arrays TaqMan Custom Arrays TaqMan Array Human Endogenous Control Plate Custom Plus TaqMan RNA Assays 96 TaqMan Gene Expression Assays 384 TaqMan 384 12 384 1 8 TaqMan 32 TaqMan Gene Expression Assays 26-27 28 28 29 24 6
mirna non-coding RNA Megaplex RT Primer Pools for TaqMan MicroRNA Assays mirna cdna Multiplex RT 1 mirna 39 TaqMan MicroRNA Assays TaqMan mirna mirna TaqMan Array MicroRNA Card TaqMan MicroRNA Assays 384 TaqMan 8 TaqMan Non-coding RNA Assays TaqMan Non-coding RNA Assays non-coding 42 TaqMan Pri-miRNA Assays mirnaprimary mirna pri-mirna 42 40 41 SNP TaqMan Pre-Designed SNP Genotyping Assays SNP SNP 2 TaqMan PCR 45 TaqMan Drug Metabolism Genotyping Assays TaqMan Copy Number Assays Custom TaqMan Copy Number Assays & Custom Plus TaqMan Copy Number Assays TaqMan Copy Number Reference Assays TaqMan Mutation Detection Assays 221 2,700 Genotyping TaqMan CNV 160 18 TaqMan CNVs TaqMan Copy Number Assays TaqMan Copy Number Reference Assays PCR 3 4 TaqMan Copy Number Assays CNVs TaqMan Copy Number Reference Assays TaqMan Copy Number Assays Custom TaqMan Copy Number Assays TaqMan Competitive Allele-Specific TaqMan PCR castpcr MGB TaqMan Protein Assay Open Kit / TaqMan Protein Assays Core Reagents Kit with Master Mix TaqMan Protein Assay 52-53 2 TaqMan 5 TaqMan Custom Plating Service TaqMan Gene Expression Assays TaqMan MicroRNA Assays 96 384 / / 96 Standard 96 Fast 384 TaqMan Gene Expression Assays Inventoried, Made-to-Order, and Custom 24 TaqMan MicroRNA Assays Inventoried, Made-to-Order, and Custom 40 TaqMan Non-coding RNA Assays Inventoried, Made-to-Order 42 TaqMan SNP Genotyping Assays Predesigned Custom and DME 45 TaqMan Copy Number Assays Predesigned Custom Plus or Custom 49 45 49 49 49 48 7
TaqMan TaqMan 96 384 384 TaqMan Array OpenArray 1 PCR 96 384 1 Applied Biosystems Quant Studio 12K Flex ViiA 7 7900HTPCR TaqMan Array 1 Applied Biosystems Quant Studio 12K Flex OpenArray PCR / PCR PCR StepOne PCR 48 3 Fast StepOnePlus TM StepOnePlus 96 4 Fast PCR VeriFlex TM QuantStudio TM 12K Flex 96 96 Fast 384 TaqMan Array OpenArray 5 1 1 12,000 OptiFlex LED 21 microrna PCR ViiA TM 7 PCR 21 OptiFlex 96 96 Fast 384 TaqMan Array 4 QuantStudio TM 3D PCR 20,000 NEW 8
PCR QuantStudio 12K Flex PCR PCR PCR PCR PCR PCR PCR PCR PCR 3 QuantStudio 12K Flex PCR PCR QuantStudio 12K Flex PCR OpenArray QuantStudio 12K Flex PCR QuantStudio PCR 1 49,000 PCR DNA DNA PCR 3. QuantStudio 12K Flex TaqMan OpenArray PCR BK AcroMetrix BK Viral StandardDNA OpenArray 4 256 8 9 10 12 5 8 PCR PCR 15,800 / L QuantStudio 12K FlexPCR QuantStudio 12K Flex OpenArray OpenArray 2 OpenArray Accufill DigitalSuite Windows 7 1 QuantStudio Digital PCR Kit, 10-pack 4470184 QuantStudio Digital 10 166,700 2X TaqMan OpenArray Digital PCR Master Mix 5 ml QuantStudio 12K Flex OpenArray Accessories Kit 10 QuantStudio Digital PCR Kit, 4-pack 4470185 QuantStudio PCR 4 2X TaqMan OpenArray Digital PCR Master Mix 5 ml 4469576 66,700 QuantStudio 12K Flex OpenArray Digital PCR Starter Kit 4469607 OpenArray Digital PCR Plate 2 TaqMan OpenArray Digital PCR Master Mix 1.5 ml Human MaleDNA TaqMan Assay RNase P OpenArray Accessories Kit 222,200 DigitalSuite Software 4472103 PCR 10 400,000 QuantStudio 12K Flex OpenArray Accessories Kit 4469576 QuantStudio 12K Flex OpenArray PCR Cases 10 QuantStudio 12K Flex OpenArray Immersion Fluids 10 QuantStudio 12K Flex OpenArray Case Sealing Glues 2 38,900 9
RNA RNA PureLink RNA Kit PureLink RNA Mini Kit RNA RNA 1,000µg 100µg DNase Set DNA RNA µg 1,000 900 800 700 600 500 400 300 200 100 0 PureLink RNA Mini Kit Competitor s midi kit Competitor s mini kit 25 100 200 Tissue mg 4. RNA PureLink RNA Mini Kit RNA PureLink RNA Mini Kit PureLink RNA Mini Kit 10 12183020 9,200 50 12183018A 28,500 250 12183025 120,000 PureLink RNA Micro Kit 50 12183016 40,000 PureLink Homogenizer 50 12183026 10,000 PureLink DNase Set 50 12185010 11,500 RNA TRIzol Reagent TRIzol Reagent DNA RNA TRIzol LS Reagent RNA TRIzol Reagent 100 L 15596026 24,400 200 L 15596018 43,300 4 200 L 15596018SP 158,000 TRIzol LS Reagent 100 L 10296010 24,200 200 L 10296028 42,800 RNA TRIzol Plus RNA Purification System TRIzol Plus RNA Purification System Purelink RNA Mini Kit No.1 TRIzol 45RNA TRIzol RNA TRIzol Plus RNA Purification System TRIzol Reagent 100mL 15596026 PureLink RNA Mini Kit 50 12183018A 50 12183555 50,000 3. RNA TRIzol Reagent PureLink Kits TRIzol Plus RNA Purification System MagMAX Kits TRIzol + 60 20 45 45 100mg 1 10 8 (1mL ) 200mg 5 10 6 1 10 8 200mg 5 10 8 100mg 5 10 6 10
RNA MagMax RNA RNA MagMax RNA MagMAX Total Nucleic Acid 100 AM1840 110,000 Isolation Kit MagMAX -96 Blood RNA Isolation Kit 96 AM1837 65,800 MagMAX -96 Total RNA Isolation Kit 96 AM1830 59,500 MagMAX Viral RNA Isolation Kit 50 AM1939 40,000 MagMAX -96 Viral RNA Isolation Kit 96 AM1836 59,900 RNA Single Place Magnetic Stand 1 AM10026 12,400 Magnetic Stand-96 1 AM10027 59,800 96 well Magnetic-Ring Stand 1 AM10050 124,000 RNA RNAqueous Kit RNAqueous Kit RNA 20 RNA DNA RNARNAqueous -4PCR Kit RNAqueous Kit 50 AM1912 36,400 RNAqueous Midi Kit 15 AM1911 26,500 RNAqueous -4PCR Kit 30 AM1914 35,600 RNAqueous -96 Kit 192 AM1920 84,000 RNAqueous -96 Automated Kit 4 96 AM1812 134,000 RNAqueous -Micro Kit 50 AM1931 42,400 RNA RNAlater Solution RNAlater Solution RNA RNAlater Solution RNAlater Solution 100mL AM7020 16,000 250mL AM7024 30,000 500mL AM7021 52,000 50 1.5mL AM7022 38,500 20 5mL AM7023 38,500 RNA RNAlater -ICE RNAlater -ICE RNA RNAlater -ICE -20 RNA RNAlater -ICE 25mL AM7030 16,700 DNA TURBO DNA-free Kit TURBO DNA-free Kit DNA TURBO DNase TURBO DNase DNase I DNA DNase I 6 RT-PCR false positive DNA TURBO DNA-free Kit 50 AM1907 18,600 RNA THE RNA Storage Solution 10 1mL AM7000 7,400 50mL AM7001 7,400 TE Buffer ph7.0 10 1mL AM9860 7,400 50mL AM9861 7,400 0.1 mm EDTA 50mL AM9912 7,400 11
Cells-to-CT Kit RNA Cells-to-CT Kit Cells-to-CT Kit RNA PCR RNA Cells-to-CT Kit sirnamicrorna mirna gdna PCR DNase 7 10 100,000 RNA TaqMan Gene Expression Cells-to-CT Kit TaqMan Fast Cells-to-CT Kit Power SYBR Green Cells-to-CT Kit Fast SYBR Green Cells-to-CT Kit TaqMan PreAmp Cells-to-CT Kit mirna TaqMan MicroRNA Cells-to-CT Kit 5. RNA 6. Cells-to-CT Kit PCR Cells-to-CT CTs HeLa RNA Cells-to-CT Kit 128 TaqMan Gene Expression Assays Applied Biosystems 7900HT PCR Cells-to-CT Kit Ct RNACt Purified RNA C T s 12
TaqMan TaqMan Gene Expression Cells-to-CT Kit TaqMan Gene Expression Cells-to-CT Kit RNA RNA DNA cdna PCR TaqMan Gene Expression Master Mix TaqMan Gene Expression Cells-to-CT Kit 40 4399002 74,000 100 AM1728 146,000 400 AM1729 560,000 * TaqMan Cells-to-CT Control Kit/Stop Solution TaqMan Cells-to-CT Control Kit TaqMan Gene Expression Cells-to-CT Kit PCR TaqMan Cells-to-CT Control Kit 100 4386995 66,000 Cells-to-CT Stop Solution 1 ml 4402960 32,000 SYBR Green Power SYBR Green / Fast SYBR Green Cells-to-CT Kit SYBR Green Cells-to-CT Kits RNA SYBR Green DNA PCR Power SYBR Green Master Mix Fast SYBR Green Master Mix Power SYBR Green Cells-to-CT Kit Power SYBR Green Cells-to-CT Kit 40 4402953 69,000 100 4402954 122,000 400 4402955 465,000 * Fast SYBR Green Cells-to-CT Kit Fast SYBR Green Cells-to-CT Kit 100 4402956 122,000 400 4402957 470,000 * SYBR Green Cells-to-CT Control Kit SYBR Green Cells-to-CT Control Kit 100 4402959 37,200 TaqMan Fast Cells-to-CT Kit TaqMan Fast Cells-to-CT Kit RNA Fast PCR PCR TaqMan Fast Universal PCR Master Mix TaqMan Fast Cells-to-CT Kit 100 4399003 146,000 * mirna TaqMan MicroRNA Cells-to-CT Kit TaqMan MicroRNA Cells-to-CT Kit RNA mirna mirna TaqMan MicroRNA Cells-to-CT Kit 100 4391848 190,000 * TaqMan PreAmp Cells-to-CT Kit TaqMan PreAmp Cells-to-CT Kit TaqMan Gene Expression Cells-to-CT Kit RNA TaqMan Gene Expression Master Mix cdna TaqMan PreAmp Master Mix TaqMan PreAmp Cells-to-CT Kit 40 4387299 276,000 * 13
Cell-to-CT Kit Ambion Single Cell-to-CT Kit Ambion Single Cell-to-CT Kit 1 10 RNA 5 Pre-Amplification PCR RNA Ambion Single Cell Lysis Kit RNA Ambion Single Cell Lysis Kit RNA RNA PCR Single Cell-to-CT Kit 28 26 24 22 20.4 21.6 20 18 16 13.6 14 12 1 Cell Equivalent Single Cells 100 Cells 7. Single Cell-to-CT Kit 100 6.8 CT CT T 1 Ambion Single Cell-to-CT Kit 50 4458237 170,000 400 4458236 980,000 Ambion Single Cell Lysis Kit 100 4458235 74,000 * DNA 10 L Single Cell Solution 1 10 5 9. 2 1 L Stop Solution 2 Cells-to-CpG Bisulfite Conversion Kit Cells-to-CpG Bisulfite Conversion Kit DNA FFPE DNA 2 8. 10 1. Single Cell Lysis Buffer 25 5 2. PBS / 5 3. PBS RT 95 10 cdna 14 Pre-Amplification B2M GAPDH HPRT1 TGFB1 5 Ct Cells-to-CpG Bisulfite Conversion Kit 50 4445555 36,000 2 96 4445554 62,000 Cells-to-CpG Bisulfite Conversion & 20 4445553 28,500 Quantitation Control Kit Cells-to-CpG Methylated and Unmethylated gdna Control Kit 50µL 4445552 42,000 14
1 RT-PCR TaqMan RNA-to-CT 1-Step Kit TaqMan RNA-to-CT 1-Step Kit 1 TaqMan 1 1 TaqMan RNA TaqMan Fast Virus 1-Step Master Mix 21 1 AT GC TaqMan Gene Expression Assays 24 TaqMan RNA-to-CT 1-Step Kit, 1mL 40 4392653 25,000 TaqMan RNA-to-CT 1-Step Kit, 5mL 200 4392938 76,000 TaqMan RNA-to-CT 1-Step Kit, 2,000 4392656 688,000 5mL 10 TaqMan RNA-to-CT 1-Step Kit 1 4393463 TaqMan RNA-to-CT 1-Step Kit 1 4393464 TaqMan One-Step RT-PCR Mater Mix Reagents Kit RT 1 RT-PCR TaqMan One-Step RT-PCR Master 200 4309169 89,000 Mix Reagents Kit 10-Pack, TaqMan One-Step RT-PCR 2,000 4313803 860,000 Master Mix Reagents Kit TaqMan One-Step RT-PCR Master Mix Reagents Kit 1 4310299 Power SYBR Green RNA-to-CT 1-Step Kit Power SYBR Green RNA-to-CT 1-Step Kit PCR 1 SYBR Green Rn 10 1 0.1 0.01 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Cycle Power SYBR Green RNA-to-CT 40 4391178 25,000 1-Step Kit, 1mL Power SYBR Green RNA-to-CT 200 4389986 67,000 1-Step Kit, 5mL Power SYBR Green RNA-to-CT 1 4391003 1-Step Kit Power SYBR Green RNA-to-CT 1-Step Kit 1 4391112 Derivative Reporter (-Rn) 3.5 3.0 2.5 2.0 1.5 1.0 0.5 65.0 70.0 75.0 80.0 85.0 90.0 95.0 Temperature (ºC) 10. Power SYBR Green RNA-to-CT 1-Step Kit GAPDH RNA BOSC23 Applied Biosystems StepOnePlus PCR 3 TaqMan EZ RT-PCR Core Reagents RT 1 RT-PCR TaqMan EZ RT-PCR Core Reagents 200 N8080236 122,000 10-Pack, TaqMan EZ RT-PCR Core 2,000 403028 1,120,000 Reagents TaqMan EZ RT-PCR Kit 1 402877 15
PCR SuperScript VILO SuperScript VILO Variable Input Linear Output SuperScript RT PCR mrna cdna RNA SuperScript VILO Master Mix 1 VILO SuperScript VILO PCR cdna PCR 1pg 2.5 g RNA cdna RNA cpm 30000 25,687 25000 20000 15000 9,310 10000 5000 2,965 0 S VILO A 11. cdna 32 P-dCTP cdna S A SuperScript VILO (VILO) A 9 VILO cdna 13. cdna PCR TaqMan TfR Target SuperScript VILO cdna Synthesis Kit SuperScript VILO cdna Synthesis Kit 50 11754050 33,300 250 11754250 141,400 SuperScript VILO Master Mix SuperScript VILO Master Mix 50 11755050 38,400 250 11755250 161,600 500 11755550 292,900 RNA cdna RNA cdna 12. RNA cdna VILO RNA cdna 4. High Capacity cdna High Capacity RNA-tocDNA Kit cdna Synthesis Kit Master Mix SuperScript VILO SuperScript VILO Reverse Transcriptase Kit MultiScribe MultiScribe Superscript III Superscript III 37 C 37 C 42 C 42 C (Total RNA) 20ng-2 g 20ng-2 g 1pg-2.5 g 1pg-2.5 g RNase H - - 5 2 2 1 16
High Capacity RNA-to-cDNA Kit High Capacity RNA-to-cDNA Kit PCR cdna RNA PCRHigh Capacity RNA-to-cDNA Kit RNA cdna 2 30 60 2µg RNA 2 RT-PCR * High Capacity RNA-to-cDNA Kit 50 4387406 19,800 High Capacity RNA-to-cDNA Kit 1 4387951 High Capacity RNA-to-cDNA Kit 1 4392339 *20µL High Capacity cdna Reverse Transcription Kit High Capacity cdna Reverse 200 4368814 29,600 Transcription Kit 1,000 4368813 125,000 High Capacity cdna Reverse Transcription Kit, with RNase Inhibitor 200 4374966 37,000 1,000 4374967 168,000 High Capacity cdna Reverse 1 4375575 Transcription Kit RNase Inhibitor, 20units/µL 100 N8080119 12,600 *20µL TaqMan Reverse Transcriation Reagents TaqMan Reverse Transcription 200 N8080234 57,400 Reagents 10-Pack, TaqMan Reverse Transcription Reagents 2,000 4304134 500,000 / / MultiScribe Reverse Transcriptase 5,000units 4311235 12,100 RNase Inhibitor, 20units/µL 2,000units N8080119 12,600 Random Hexamers, 5nmoles 0.1mL N8080127 11,000 Oligo d(t) 16 Primers, 5nmoles 0.1mL N8080128 11,000 GeneAmp dntp Blend, 10mM 1mL N8080260 10,000 GeneAmp dntp Blend, 12.5mM with dutp 100 N8080270 15,200 AmpErase Uracil N-glycosylase 100units N8080096 25,000 UNG 20% Glycerol Solution, Molecular 100mL 402929 12,000 Biology Grade TaqMan Control Total RNA Human 100µL 4307281 14,000 50ng/µL TaqMan Control Genomic DNA Human 10ng/µL, 2 100µL 4312660 12,000 17
TaqMan Fast Fast PCR 4045 Fast Fast 40 TaqMan Fast Advanced Master Mix 55 40 FastFast PCR Fast Fast PCR Rn 10 1 0.1 0.01 12 14 16 18 20 22 24 26 28 30 Cycle Number TaqMan Fast Advanced Master Mix 40 TaqMan Fast Universal PCR Master Mix TaqMan Fast Universal PCR Master Mix TaqMan Fast PCR Fast PCR Fast PCR Fast AmpliTaq Fast DNA Polymerase 72 PCR TaqMan Assays TaqMan Array Fast Fast Fast TaqMan Fast Universal PCR Master Mix 250 4352042 32,000 2X, No AmpErase UNG 2-Pack, TaqMan Fast Universal PCR 500 4366072 62,000 Master Mix 2X, No AmpErase UNG 5-Pack, TaqMan Fast Universal PCR 1,250 4366073 154,000 Master Mix 2X, No AmpErase UNG 10-Pack, TaqMan Fast Universal PCR Master Mix 2X, No AmpErase UNG 2,500 4364103 294,000 TaqMan Fast Universal PCR Master Mix 5,000 4367846 530,000 50mL TaqMan Fast Universal PCR Master Mix 1 4351891 *20µL TaqMan Fast Advanced Master Mix, 100 4444556 14,000 1mL TaqMan Fast Advanced Master Mix, 500 4444557 63,000 5mL TaqMan Fast Advanced Master Mix, 1,000 4444963 122,000 2 5mL TaqMan Fast Advanced Master Mix, 2,500 4444964 295,000 5 5mL TaqMan Fast Advanced Master Mix, 5,000 4444965 570,000 10 5mL TaqMan Fast Advanced Master Mix, 5,000 4444558 558,000 50mL *20µL 18
TaqMan 1 TaqMan Universal Master Mix II TaqMan Universal Master Mix II microrna mirna SNP CNV TaqMan Universal Master Mix II 1 RNase P TaqMan Universal Master Mix II PCR PCR 24 SNP mirna TaqMan Assays TaqMan Assays PCR UNG 2 14. PCR TaqMan Universal Master Mix II, with UNG TaqMan Universal Master Mix II, 40 4440042 14,000 with UNG 1mL TaqMan Universal Master Mix II, 200 4440038 63,000 with UNG 5mL TaqMan Universal Master Mix II, 400 4440044 122,000 with UNG 2 5mL TaqMan Universal Master Mix II, 1,000 4440045 295,000 with UNG 5 5mL TaqMan Universal Master Mix II, 2,000 4440046 570,000 with UNG 10 5mL TaqMan Universal Master Mix II, with UNG 50mL 2,000 4440039 558,000 TaqMan Universal Master Mix II, no UNG TaqMan Universal Master Mix II, 40 4440043 14,000 no UNG 1mL TaqMan Universal Master Mix II, 200 4440040 58,000 no UNG 5mL TaqMan Universal Master Mix II, 400 4440047 112,000 no UNG 2 5mL TaqMan Universal Master Mix II, 1,000 4440048 270,000 no UNG 5 5mL TaqMan Universal Master Mix II, 2,000 4440049 510,000 no UNG 10 5mL TaqMan Universal Master Mix II, no UNG 50mL 2,000 4440041 500,000 *50µL PCR24 PCR TaqMan Universal Master Mix II PCR PCR 30 24 15. 24 19
TaqMan TaqMan Gene Expression Master Mix TaqMan Gene Expression Master Mix PCR GC AT PCR TaqMan Universal PCR Master Mix TaqMan Universal PCR Master Mix TaqMan 5' PCR AmpliTaq Gold DNA Polymerase 72 TaqMan Gene Expression Master Mix, 40 4370048 14,000 1mL TaqMan Gene Expression Master Mix, 200 4369016 63,000 5mL TaqMan Gene Expression Master Mix, 400 4369514 122,000 2 5mL TaqMan Gene Expression Master Mix, 1,000 4369510 295,000 5 5mL TaqMan Gene Expression Master Mix, 2,000 4369542 570,000 10 5mL TaqMan Gene Expression Master Mix, 2,000 4370074 558,000 50mL TaqMan Gene Expression Master Mix 1 4371135 TaqMan Gene Expression Master Mix 1 4371134 *50µL RNA PCRWater Nuclease free Water Nuclease-free Water 500mL AM9930 12,400 1,000mL AM9932 20,600 10 50mL AM9937 15,500 100mL AM9938 5,200 5 100mL AM9939 18,500 TaqMan Universal PCR Master Mix 200 4304437 68,000 2-Pack, TaqMan Universal PCR Master Mix 400 4364338 125,000 4304437 2 5-Pack, TaqMan Universal PCR Master Mix 1,000 4364340 305,000 4304437 5 10-Pack, TaqMan Universal PCR Master Mix 2,000 4305719 600,000 4304437 10 TaqMan Universal PCR Master Mix, 10-Pack 2,000 4318157 600,000 5mL 10 TaqMan Universal PCR Master Mix, 50 ml 2,000 4326708 590,000 TaqMan Universal PCR Master Mix 1 4304449 *50µL TaqMan Universal PCR Master Mix, No AmpErase UNG AmpErase UNG TaqMan 5' PCR AmpliTaq Gold DNA Polymerase TaqMan Universal PCR Master Mix, 200 4324018 63,000 No AmpErase UNG TaqMan Universal PCR Master Mix, 400 4364341 116,000 No AmpErase UNG, 2-Pack TaqMan Universal PCR Master Mix, 1,000 4364343 278,000 No AmpErase UNG, 5-Pack TaqMan Universal PCR Master Mix, 2,000 4324020 540,000 No AmpErase UNG, 10-Pack TaqMan Universal PCR Master Mix, 2,000 4326614 530,000 No AmpErase UNG, 50 ml TaqMan Universal PCR Master Mix 1 4304449 *50µL 20
PCR TaqMan Fast Virus 1-Step Master Mix PCR RNA DNA 1 4 RNA / DNA PCR Fast RT-PCR PCR PCR TaqMan Fast Virus 1-Step Master Mix Fast PCR TaqMan Fast Virus 1-Step Master Mix PCR 4 Fast TaqMan EZ RT-PCR Core Reagents TaqMan RNA-to-C T 1-Step Kit TaqMan Fast Virus 1-Step Master Mix 0 20 40 60 80 100 120 140 TaqMan PreAmp Master Mix TaqMan PreAmp Master Mix cdna PrePCR PrePCR TaqMan Gene Expression Assays 100 1ngcDNA 200 PCR TaqMan PreAmp Master Mix 40 4391128 124,000 TaqMan PreAmp Master Mix Kit 1 4384267 158,000 PreAmp Master Mix (4391128) TaqMan Gene Expression Master Mix, 5mL (4369016) TaqMan PCR Core Reagents Kit TaqMan PCR Core Reagents Kit TaqMan PCR TaqMan PCR Core Reagents Kit with 200 N8080228 64,000 AmpliTaq Gold 10-Pack, TaqMan PCR Core Reagent Kit 2,000 402930 570,000 TaqMan PCR Reagent Kit 1 402823 *50µL TaqMan Gold with Buffer A Pack 1000 Reaction TaqMan Gold with Buffer A Pack 1,000 4304441 172,000 *50µL TaqMan Reagents Starter Kit TaqMan Reagents Starter Kit 1Kit 4352405 35,000 TaqMan Universal Master Mix, No UNG 1.25mL TaqMan Gene Expression Assay, Eukaryotic 18S rrna, 20X 0.3mL Human Raji cdna, 25ng/µL 0.05mL 16. 4 Fast TaqMan Fast Virus 1-Step Master Mix 1 1 TaqMan Fast Virus 1-Step Master Mix TaqMan Fast Virus 1-Step Master Mix, 200 4444432 49,600 1mL TaqMan Fast Virus 1-Step Master Mix, 1,000 4444434 152,000 5 1mL TaqMan Fast Virus 1-Step Master Mix, 10mL 2,000 4444436 295,000 *20µL 21
SYBR Green Power SYBR Green PCR Master Mix Power SYBR Green PCR Master Mix 2 DNA cdna RNA PCR SYBR Green PCR 2 Power SYBR Green PCR Master Mix, 40 4368577 9,000 1mL Power SYBR Green PCR Master Mix, 200 4367659 35,000 5mL 2-Pack, Power SYBR Green PCR Master 400 4368706 68,000 Mix, 2 5mL 5-Pack, Power SYBR Green PCR Master 1,000 4368702 165,000 Mix, 5 5mL 10-Pack, Power SYBR Green PCR Master 2,000 4368708 320,000 Mix, 10 5mL Power SYBR Green PCR Master Mix, 2,000 4367660 300,000 50mL Power SYBR Green PCR Master Mix 1 4367218 *50µL Fast SYBR Green Master Mix Fast SYBR Green Master Mix Fast PCR Fast PCR PCR Fast SYBR Green Master Mix, 1mL 100 4385610 9,000 Fast SYBR Green Master Mix, 5mL 500 4385612 35,000 2-Pack, Fast SYBR Green Master Mix, 1,000 4385616 68,000 2 5mL 5-Pack, Fast SYBR Green Master Mix, 2,500 4385617 165,000 5 5mL 10-Pack, Fast SYBR Green Master Mix, 5,000 4385618 320,000 10 5mL Fast SYBR Green Master Mix, 50mL 5,000 4385614 300,000 Fast SYBR Green Master Mix 1 4385371 Fast SYBR Green Master Mix 1 4385372 *20µL 1.0e+002 1.0e+001 Delta Rn vs Cycle 50 ng 5 ng 500 pg 50 pg 5 pg 18. Fast SYBR Master Mix CT Delta Rn 1.0e+000 1.0e-001 1.0e-002 5 10 15 20 25 30 35 40 Cycle Number Standard Curve 34 32 5 pg Slope = -3.28 30 50 pg 28 R 2 = 0.997 26 500 pg 24 22 5 ng 20 50 ng 18 16 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 Log Concentration 17. Human gdna -actin SYBR Green PCR Master Mix SYBR Green PCR Master Mix SYBR Green PCR 2 SYBR Green PCR Master Mix, 1mL 40 4344463 10,000 SYBR Green PCR Master Mix, 5mL 200 4309155 49,000 SYBR Green PCR Master Mix, 50mL 2,000 4334973 425,000 10-Pack, SYBR Green PCR Master Mix, 2,000 4312704 440,000 10 5mL SYBR Green PCR Master Mix 1 4310251 *50µL SYBR Green PCR Core Reagents SYBR Green PCR Core Reagents 200 4304886 64,000 10-Pack, SYBR Green PCR Core 2,000 4306736 568,000 Reagents SYBR Green PCR Core Reagents 1 4304965 *50µL 22
A SYBR Select Master Mix SYBR Select Master Mix PCR Rn Cycle DNA PCR B Rn 1E01 1E00 SYBR GreenER 0.1 Cycle PCR 72 PCR 72 PCR UDG 19. cdna A cdna PGK1 PCR 100 ng cdna B 1 ng 2 ng cdna ViiA 7 PCR A PCR Applied Biosystems PCR SYBR Select Master Mix, mini Pack 100 4472903 7,700 1mL SYBR Select Master Mix, 1 Pack 500 4472908 29,800 5mL SYBR Select Master Mix, 2 Pack 1,000 4472918 57,800 2 5mL SYBR Select Master Mix, 5 Pack 2,500 4472919 140,000 5 5mL SYBR Select Master Mix, 10 Pack 5,000 4472920 272,000 10 5mL SYBR Select Master Mix, Bulk Pack 50mL 5,000 4472913 272,000 *20µL B Distribution 0.6 0.5 0.4 0.3 0.2 0.1 0 28 30 32 34 36 38 40 20. qpcr C t A DNA 1 1 0 DNA PCR 50 DNA B RNase PCt Ct SYBR Select Master Mix ViiA 7 PCR 23
TaqMan TaqMan Gene Expression Assays TaqMan Gene Expression Assays PCR & 130 Exon RNAi FAM TaqMan Gene Expression Assays, Inventoried 250 4331182 29,800 TaqMan Gene Expression Assays, Small, Made-to-Order TaqMan Gene Expression Assays, Medium, Made-to-Order TaqMan Gene Expression Assays, Large, Made-to-Order VIC 360 4351372 45,000 750 4351370 74,800 2,900 4351368 148,500 TaqMan Gene Expression Assays, 360 4448489 45,000 Small_VIC, Made-to-Order TaqMan Gene Expression Assays, 750 4448490 74,800 Medium_VIC, Made-to-Order TaqMan Gene Expression Assays, Large_VIC, Made-to-Order 2,900 4448491 148,500 (Primer Limited)TaqMan Assays VIC TaqMan Gene Expression Assays, 360 4448484 45,000 Small_VIC, PL Made-to-Order TaqMan Gene Expression Assays, 750 4448485 74,800 Medium_VIC, PL Made-to-Order TaqMan Gene Expression Assays, Large_VIC, PL Made-to-Order 2,900 4448486 148,500 *20µL Inventoried Made-to-Order 60 www.lifetechnologies.com/taqmanjp 5. TaqMan Gene Expression Assays 2011 10 Species Number of Assays H. sapiens (Human) 212,207 M. musculus (Mouse) 185,566 R. norvegicus (Rat) 151,955 O. sativa (Rice) 99,822 A. thaliana (Arabidopsis) 97,879 C. elegans (Nematode) 92,687 B. taurus (Cow) 67,104 M. mulatta (Rhesus Monkey) 66,480 D. rerio (Zebrafish) 63,712 X. tropicalis (Frog) 56,764 C. familiaris (Dog) 55,202 G. gallus (Chicken) 48,432 Custom Plus TaqMan RNA Assays Custom Plus TaqMan RNA Assays TaqMan Gene Experssion Assays Custom Plus TaqMan RNA Assays Custom Plus TaqMan RNA Assay, Small 360 4441114 45,000 20 Custom Plus TaqMan RNA Assay, Medium 20 750 4441117 90,900 Custom Plus TaqMan RNA Assay, Large 60 Species Number of Assays D. melanogaster (Fruit Fly) 41,607 Z. mays (Sweet Corn) 38,446 S. scrofa (Pig) 9,868 S. pombe (Fission Yeast) 6,538 O. cuniculus (Rabbit) 5,924 S. cerevisiae (Baker's Yeast) 5,525 G. max (Soybean) 3,456 E. caballus (Horse) 2,970 C. porcellus (Guinea Pig) 2,037 V. vinifera (Grape) 965 T. aestivum (Wheat) 760 Total Number of Assays 1,315,906 Custom TaqMan Gene Expression Assays Custom TaqMan Gene Expression Assays TaqMan Gene Expression Assays TaqMan Gene Expression Assays TaqMan Custom TaqMan Gene 50µL 140 4331348 45,000 Expression Assays, S-scale 20µL 360 Custom TaqMan Gene 50µL 300 4332078 90,900 Expression Assays, M-scale 20µL 750 Custom TaqMan Gene Expression Assays, L-scale 50µL 1,160 20µL 2,900 4332079 161,600 2,900 4441118 161,600 24
TaqMan 96 TaqMan Array Plate 96 - Fast TaqMan Array Plate TaqMan Gene Expression Assays 24 96 5 Fast 10 TaqMan Gene Expression Assays 6 96 PCR TaqMan Standard : 7900HT / ViiA 7 / 7500 / 7300 / QuantStudio 96 Fast : 7900HT / ViiA 7 /7500Fast /StepOnePlus / QuantStudio Fast96 60 TaqMan Array Plate www.lifetechnologies.com/taqmanjp ** * TaqMan Array Plates, Format 8 (Standard) TaqMan Array Plates, Format 8 (Fast) TaqMan Array Plates, Format 16 (Standard) TaqMan Array Plates, Format 16 (Fast) TaqMan Array Plates, Format 16 + Endogenous Controls (Standard) TaqMan Array Plates, Format 16 + Endogenous Controls (Fast) TaqMan Array Plates, Format 32 (Standard) TaqMan Array Plates, Format 32 (Fast) TaqMan Array Plates, Format 32 + Endogenous Controls (Standard) TaqMan Array Plates, Format 32 + Endogenous Controls (Fast) TaqMan Array Plates, Format 48 (Standard) TaqMan Array Plates, Format 48 (Fast) TaqMan Array Plates, Format 48 + Endogenous Controls (Standard) TaqMan Array Plates, Format 48 + Endogenous Controls (Fast) TaqMan Array Plates, Format 96 (Standard) TaqMan Array Plates, Format 96 (Fast) TaqMan Array Plates, Format 96 + Endogenous Controls (Standard) TaqMan Array Plates, Format 96 + Endogenous Controls (Fast) 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 6 12 18 4413266 30,300 26,300 22,200 4413263 30,300 26,300 22,200 4413264 34,400 30,300 26,300 4413261 34,400 30,300 26,300 4413265 34,400 30,300 26,300 4413262 34,400 30,300 26,300 4391528 45,500 39,400 34,400 4413259 45,500 39,400 34,400 4391529 45,500 39,400 34,400 4413260 45,500 39,400 34,400 4391526 50,500 45,500 39,400 4413257 50,500 45,500 39,400 4391527 50,500 45,500 39,400 4413258 50,500 45,500 39,400 4391524 59,600 50,500 45,500 4413255 59,600 50,500 45,500 4391525 59,600 50,500 45,500 4413256 59,600 50,500 45,500 * 1 6612 18 24 ** Candidate Endogenous Control GAPDH HPRT1 GUSB 3 25
TaqMan 96 TaqMan Array Gene Signature 96-Well Plate TaqMan Array Gene Signature 96-Well Plate TaqMan Gene Expression Assays 96 1 96 Standard Fast Human Alzheimer's Disease 4414070 4418715 Human Angiogenesis 4414071 4418716 Human Apoptosis 4414072 4418717 Human Immune Response 4414073 4418718 Human Inflammation 4414074 4418719 Human Phosphodiesterase 4414075 4418720 Human Protein Kinase Pathways 4414076 4418721 Human Stem Cell Pluripotency 4414077 4418722 Mouse Alzheimer's Disease 4414078 4418723 Mouse Immune Response 4414079 4418724 Mouse Stem Cell Pluripotency 4414080 4418725 Rat Inflammation 4414081 4418726 Rat Phosphodiesterase 4414082 4418727 Human Androgens 4414083 4418728 Human Chemokines 4414084 4418729 Human CYP450 & other Oxygenases 4414085 4418730 Human Diabetes 4414086 4418731 Human Estrogens 4414087 4418732 Human Heat Shock Proteins 4414088 4418733 Human Hedgehog Pathway 4414089 4418734 Human Hypoxia 4414090 4418735 Human LDL Pathways 4414091 4418736 Human Ligand Gated Ion Channels 4414092 4418737 Human MAPK Pathways 4414093 4418738 Human Neurotransmitters 4414094 4418739 Human NFkB Pathway 4414095 4418740 Human Osteogenesis 4414096 4418741 Human TGF Pathway 4414097 4418742 Human Tumor Metastasis 4414098 4418743 Human Voltage-Gated Ion Channels 4414099 4418744 Human WNT Pathway 4414100 4418745 Human 14-3-3 Induced Intracellular Signaling 4414101 4418746 Human Activation of camp-dependent PKA 4414102 4418747 Human HOX Genes 4414103 4418748 Human Antigen Processing and Presentation by 4414104 4418749 MHCS Human Apoptosis via Death Receptors 4414105 4418750 Human Apoptotic Pathways Triggered By HIV1 4414106 4418751 Mouse ABC Transporters 4414107 4418752 Human Nuclear Receptors 4414108 4418753 Human Cell Surface Markers 4414109 4418754 Human BMP Pathway 4414110 4418755 134,400 Standard Fast Human BRCA1 Pathway 4414111 4418756 Human Breast Cancer Regulations by Stathmin1 4414112 4418757 Human c-myc and Apoptosis 4414113 4418758 Human camp Pathway 4414114 4418759 Human Cardiomyocyte Differentiation by BMP 4414115 4418760 Receptors Human Cell Cycle Control of Chromosomal 4414116 4418761 Replication Human Cellular Apoptosis Pathway 4414117 4418762 Human Drug Transporters 4414118 4418763 Human Antioxidant Mechanisms 4414119 4418764 Human Complement Pathway 4414120 4418765 Human Colorectal Cancer Metastasis 4414121 4418766 Human CREB Pathway 4414122 4418767 Human Cyclins & Cell Cycle Regulation 4414123 4418768 Human Cytokine Network 4414124 4418769 Human Developmental Phases of Colorectal 4414125 4418770 Cancer Human DNA Damage Induced 14-3-3 Sigma 4414126 4418771 Signaling Human DNA Methylation & Transcriptional 4414127 4418772 Repression Human DNA Repair Mechanisms 4414128 4418773 Human EGF Pathway 4414129 4418774 Human Signal Transduction Pathways 4414130 4418775 Human enos Signaling 4414131 4418776 Human Erythropoietin Pathway 4414132 4418777 Human Extracellular Matrix & Adhesion 4414133 4418778 Molecules Human Factors for Cardiogenesis 4414134 4418779 Human FAS Signaling 4414135 4418780 Human FGF Pathway 4414136 4418781 Human GPCR Pathway 4414137 4418782 Human Guidance & Growth Cone Motility 4414138 4418783 Human Transcription Factors (Non-Hox) 4414139 4418784 Human Huntington Disease Pathway 4414140 4418785 Human IGF1R Signaling 4414141 4418786 Human IL-1 Pathway 4414142 4418787 Human IL-10 Pathway 4414143 4418788 Human IL-2 Active 8 & Quiescent T-Cell 4414144 4418789 Human IL-2 Pathway 4414145 4418790 Human AHR Pathway 4414146 4418791 Human IL-3 Pathway 4414147 4418792 26
Standard Fast Human IL-4 Pathway 4414148 4418793 Human IL-6 Pathway 4414149 4418794 Human IL-9 Pathway 4414150 4418795 Human ILK Signaling 4414151 4418796 Human inos Signaling 4414152 4418797 Human Insulin Receptor Pathway 4414153 4418798 Human Interferon Pathway 4414154 4418799 Human Intracellular Calcium Signaling 4414155 4418800 Human JAK-STAT Pathway 4414156 4418801 Human Growth Factors 4414157 4418802 Human Hematopoiesis 4414158 4418803 Human Melatonin Signaling 4414159 4418804 Human Prion Pathway 4414160 4418805 Human Molecular Mechanisms of Cancer 4414161 4418806 Human NGF Pathway 4414162 4418807 Human nnos Signaling at Neuronal Synapses 4414163 4418808 Human nnos Signaling in Skeletal Muscle 4414164 4418809 Human Notch Signaling 4414165 4418810 Human ABC Transporters 4414166 4418811 Human P53 Mediated Apoptosis 4414167 4418812 Human P53 Signaling 4414168 4418813 Human CMV & MAPK Pathways 4414169 4418814 Human Patho Rheumatoid Arthritis 4414170 4418815 Human PDGF Pathway 4414171 4418816 Human PI3K Signaling 4414172 4418817 Human PI3K Signaling in B-Lymphocyte 4414173 4418818 Human PKA Signaling 4414174 4418819 Human Cholera Infection 4414175 4418820 Human RAS Pathway 4414176 4418821 Human Pancreatic Adenocarcinoma 4414177 4418822 Human Phagocytosis of Microbes 4414178 4418823 Human RHO Family GTPases 4414179 4418824 Human RHOA Pathway 4414180 4418825 Human Pathogenesis of ALS 4414181 4418826 Human Signaling in Gap Junctions 4414182 4418827 Human NFAT & Cardiac Hypertrophy 4414183 4418828 Human SMAD Signaling Network 4414184 4418829 Human Sperm Motility 4414185 4418830 Human T-Cell Receptor and CD3 Complex 4414186 4418831 Human Telomere Extension by Telomerase 4414187 4418832 Human Thrombin Signaling 4414188 4418833 Human TNF Superfamily Pathway 4414189 4418834 1 34,400 Standard Fast Human TNFR1 Pathway 4414190 4418835 Human TNFR2 Pathway 4414191 4418836 Human Toll Comparative Pathway 4414192 4418837 Human Toll-Like Receptors Pathway 4414193 4418838 Human Transcription of mrna 4414194 4418839 Human Transcription of rrna 4414195 4418840 Human Transcription of trna 4414196 4418841 Human Transcriptional Regulatory Network in 4414197 4418842 Embryonic Stem Cell Human Ubiquitin-Proteasome Dependent 4414198 4418843 Proteolysis Human VEGF Pathway 4414199 4418844 Human Maturity-Onset Diabetes of Young 4414200 4418845 (MODY) Mouse Lipid Regulated Genes 4415461 4418846 Rat Endogenous Control 4426698 4426697 Mouse Endogenous Control 4426701 4426699 Human Endogenous Control 4396840 4426700 27
TaqMan 384 TaqMan Gene Signature Arrays TaqMan Gene Signature Arrays 384 14 TaqMan 384 384 5 Human Human Immune Array 4 4370573 202,000 Human Endogenous Control Array 2 4367563 90,900 Human Protein Kinase Array 4 4367784 202,000 Human GPCR Array 4 4367785 222,200 Human ABC Transporter Array 4 4378700 202,000 Human Apoptosis Array 4 4378701 202,000 Human Phosphodiesterase Array 4 4378705 202,000 Human Inflammation Array 4 4378707 202,000 Human Angiogenesis Array 4 4378710 202,000 Human Alzheimer's Array 4 4378713 202,000 Human Nuclear Receptor Array 4 4379961 202,000 Human Stem Cell Pluripotency Array 4 4385344 202,000 TaqMan Array Human MicroRNA A Cards v2.0 4 4398965 202,000 TaqMan Array Human MicroRNA B Cards v2.0 4 4398966 202,000 TaqMan Arrays Human MicroRNA A+B Cards Set v2.0 4 4400238 260,600 TaqMan Arrays Human MicroRNA B Cards v3.0 4 4444910 202,000 TaqMan Arrays Human MicroRNA A+B Cards v3.0 8 4444913 260,600 www.lifetechnologies.com Mouse Mouse Immune Array 4 4367786 202,000 Mouse Endogenus Control Array 2 4378702 90,900 Mouse GPCR Array 4 4378703 222,200 Mouse Alzheimer's Array 4 4378714 202,000 Mouse Stem Cell Pluripotency Array 4 4385363 202,000 TaqMan Array Rodent MicroRNA A Cards v2.0 4 4398967 202,000 TaqMan Array Rodent MicroRNA B Cards v2.0 4 4398968 202,000 TaqMan Array Rodent MicroRNA A+B Cards Set v2.0 4 4400239 260,600 Rat Rat Endogenous Control Array 2 4378704 90,900 Rat Phosphodiesterase Array 4 4378706 202,000 Rat Inflammation Array 4 4378708 202,000 Rat GPCR Array 4 4378709 222,200 TaqMan Array Rodent MicroRNA A Cards v2.0 4 4398967 202,000 TaqMan Array Rodent MicroRNA B Cards v2.0 4 4398968 202,000 TaqMan Array Rodent MicroRNA A+B Cards Set v2.0 4 4400239 260,600 TaqMan Custom Arrays Micro Fluidic Card TaqMan Custom Arrays 7900HT Fast ViiA 7 QuantStudio PCR 384 1 12 384 1 8 TaqMan Custom Arrays 7900HT ViiA 7 QuantStudio TaqMan Array Micro Fluidic Card 2µL 384 TaqMan Gene Signature Arrays 10 19 20 49 50 99 100 TaqMan Array, Format 12 4342247 68,700 56,600 45,500 40,400 TaqMan Array, Format 16 4346798 70,700 58,600 46,500 41,400 TaqMan Array, Format 24 4342249 70,700 58,600 46,500 41,400 TaqMan Array, Format 32 4346799 74,800 62,600 48,500 43,400 TaqMan Array, Format 48 4342253 74,800 62,600 48,500 43,400 TaqMan Array, Format 64 4346800 76,800 64,600 50,500 44,400 TaqMan Array, Format 96a 4342259 78,800 65,700 56,600 46,500 TaqMan Array, Format 96b 4342261 78,800 65,700 56,600 46,500 TaqMan Array, Format 192 4346802 98,000 72,700 62,600 50,500 TaqMan Array, Format 384 4342265 125,300 84,800 66,700 54,500 *TaqMan Gene Expression Assays, Inventoried 24 TaqMan Gene Expression Assays, Made-to-Order 24 60 28
TaqMan TaqMan Array Endogenous Control Plate TaqMan Array Endogenous Control Plate 32 32 3 TaqMan TaqMan Array Human Endogenous 1 4396840 34,000 Controls, 96-well Plate TaqMan Array Human Endogenous 1 4426700 34,000 Controls, Fast 96-Well Plate TaqMan Array Mouse Endogenous 1 4426701 34,000 Controls, 96-well Plate TaqMan Array Mouse Endogenous 1 4426699 34,000 Controls, Fast 96-Well Plate TaqMan Array Rat Endogenous Controls, 1 4426698 34,000 96-well Plate TaqMan Array Rat Endogenous Controls, Fast 96-Well Plate 1 4426697 34,000 6. TaqMan Array Human Endogenous Control Plate Assay ID Gene Symbol TaqMan Human Endogenous Control Array TaqMan Human Endogenous Control Array TaqMan Gene Signature Arrays 28 16 Gene Expression Assays TaqMan Human Endogenous Control Array Applied Biosystems 7900HT ViiA 7 QuantStudio TaqMan Array TaqMan Human Endogenous Control Array 2 4367563 90,900 29
TaqMan TaqMan Gene Expression Assays (Endogenous Control) TaqMan Gene Expression Assays 24 60 TaqMan Endogenous Controls TaqMan Endogenous Controls VIC FAM TaqMan Gene Expression 50µL 100 4331182 29,800 Assays, Inventoried 20µL 250 TaqMan Endogenous Control 1,000 ** 99,000 VIC /MGB * 50µL ** Human Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC /MGB FAM /MGB 18S X03205.1 Eukaryotic 18S rrna Hs99999901_s1 4319413E 4352930E ACTB NM_001101.2 actin, beta Hs99999903_m1 4326315E 4352935E GAPDH NM_002046.3 glyceraldehyde-3-phosphate dehydrogenase Hs99999905_m1 4326317E 4352934E RPLP0 NM_001002.3 ribosomal protein, large, P0 Hs99999902_m1 4326314E PPIA NM_021130.3 peptidylprolyl isomerase A (cyclophilin A) Hs99999904_m1 4326316E PGK1 NM_000291.2 phosphoglycerate kinase 1 Hs99999906_m1 4326318E B2M NM_004048.2 beta-2-microglobulin Hs99999907_m1 4326319E GUSB NM_000181.1 glucuronidase, beta Hs99999908_m1 4326320E HPRT1 NM_000194.1 hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome) Hs99999909_m1 4326321E TBP M55654.1 TATA box binding protein Hs99999910_m1 4326322E TFRC NM_003234.1 transferrin receptor (p90, CD71) Hs99999911_m1 4326323E IPO8 NM_006390.2 importin 8 Hs00183533_m1 YWHAZ NM_003406.2 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Hs00237047_m1 UBC NM_021009.4 ubiquitin C Hs00824723_m1 POLR2A NM_000937.3 polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Hs00172187_m1 HMBS NM_000190.3 hydroxymethylbilane synthase Hs00609297_m1 * VIC TaqMan Fast Universal Master Mix / 60 62 30
Mouse Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC /MGB FAM /MGB 18S X03205.1 Eukaryotic 18S rrna Hs99999901_s1 4319413E 4352930E ACTB NM_007393.1 actin, beta, cytoplasmic Mm00607939_s1 4352341E 4352933E Gapdh NM_008084.2 glyceraldehyde-3-phosphate dehydrogenase Mm99999915_g1 4352339E 4352932E Rplp2 (AK012402.1) ribosomal protein, large P2 Mm00782638_s1 Ppia NM_008907.1 peptidylprolyl isomerase A Mm02342430_g1 Pgk1 NM_008828.2 phosphoglycerate kinase 1 Mm00435617_m1 B2m NM_009735.3 beta-2 microglobulin Mm00437762_m1 Gusb NM_010368.1 glucuronidase, beta Mm00446953_m1 Hprt1 NM_013556.2 hypoxanthine guanine phosphoribosyl transferase 1 Mm00446968_m1 Tbp NM_013684.2 TATA box binding protein Mm00446973_m1 Tfrc NM_011638.3 transferrin receptor Mm00441941_m1 Ipo8 NM_001081113.1 importin 8 Mm01255158_m1 Ywhaz NM_011740.2 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Mm01158417_g1 Ubc ubiquitin C Mm01201237_m1 Polr2a NM_009089.2 polymerase (RNA) II (DNA directed) polypeptide A Mm00839493_m1 Hmbs NM_013551.2 hydroxymethylbilane synthase Mm00660262_g1 Rat Gene Symbol RefSeq Gene Name TaqMan Gene Expression Assays Assays ID TaqMan Endogenous Controls *VIC /MGB FAM /MGB 18S X03205.1 Eukaryotic 18S rrna Hs99999901_s1 4319413E 4352930E Actb NM_031144.2 actin, beta Rn00667869_m1 4352340E 4352931E Gapdh NM_017008.3 glyceraldehyde-3-phosphate dehydrogenase Rn99999916_s1 4352338E Rplp2 NM_001030021.1 ribosomal protein, large P2 Rn01479927_g1 Ppia NM_017101.1 peptidylprolyl isomerase A Rn00690933_m1 Pgk1 NM_053291.3 phosphoglycerate kinase 1 Rn00821429_g1 B2m NM_012512.1 beta-2 microglobulin Rn00560865_m1 Gusb NM_017015.2 glucuronidase, beta Rn00566655_m1 Hprt NM_012583.2 hypoxanthine guanine phosphoribosyl transferase Rn01527840_m1 Tbp NM_001004198.1 TATA box binding protein Rn01455648_m1 Tfrc M58040.1 transferrin receptor Rn01474695_m1 Hmbs NM_013168.2 hydroxymethylbilane synthase Rn00565886_m1 Ywhaz NM_013011.3 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Rn00755072_m1 Ubc NM_017314.1 ubiquitin C Rn01789812_g1 Ppib NM_022536.1 peptidylprolyl isomerase B Rn00574762_m1 Arbp NM_022402.1 acidic ribosomal phosphoprotein P0 Rn00821065_g1 * VIC TaqMan Fast Universal Master Mix / 60 62 31
RNAi RNAi RNA RNAi RNA2 RNA dsrna dsrna mrna RNAi 1. sirna RNAi 2siRNA sirna sirna33 35 RNAi RNAi RNA dsrna RNAi sirna DicerdsRNA 21 23 sirna small interfering RNA sirna RISC RNA-induced silencing complexes sirna RISC mrna RISC mrna 30 dsrna sirna Dicer sirna shrna short hairpin RNADNA RNAi 2. sirna sirna sirna sirna sirna sirna36 3. sirna mrna sirna PCR TaqMan mrna TaqMan 18 21 TaqMan 24 31 21. RNAi 32
sirna Silencer Select sirna Silencer Select sirna LNA sirna sirna 1/10 1/100 5nM LNA 22. Silencer Select sirna sirna 10siRNA 0.03 30nM sirna HeLa sirnatriplicate 48 mrna sirna mrna % sirna 5nM Silencer Select Pre-designed sirna Silencer Select Pre-designed sirna 2 70% RNA Silencer Select Validated sirna Silencer Select Validated sirna 80% mrna Silencer Select Pre-designed sirna 3 3 2 80% RNA 7. Silencer Select sirna Silencer Select Pre-designed Silencer Select Validated Custom Design Select sirna Custom Select sirna Silencer Select Negative Control #1 sirna Silencer Select Negative Control #2 sirna Silencer Select GAPDH Positive Control sirna Silencer Select Pre-designed sirna 98 Silencer Select Validated sirna RNA 80 RNA Silencer Select sirna sirna Select sirna 21 sirna Silencer Select sirna Silencer Select sirna sirna 2 Silencer Select GAPDH sirna GAPDH GAPDH sirna 23. / RNAi 33
sirna Silencer Select Pre-designed sirna Silencer Select Pre-designed sirna, 1nmol 4427037 13,000 inventoried, standard purity 5nmol 4392420 43,000 20nmol 4392421 66,000 40nmol 4392422 83,000 Silencer Select Pre-designed sirna, non-inventoried, standard purity Silencer Select Pre-designed sirna, HPLC purified * 1nmol 4 Silencer Select Validated sirna 5nmol 4390771 43,000 20nmol 4390815 66,000 40nmol 4390816 83,000 20nmol 4390817 96,000 40nmol 4390818 113,000 Silencer Select Validated sirna, 1nmol 4427038 15,000 standard purity 5nmol 4390824 55,000 20nmol 4390825 78,000 40nmol 4390826 95,000 Silencer Select Validated sirna, HPLC purified * 1nmol 4 Silencer Select Negative Control sirna 20nmol 4390821 108,000 40nmol 4390822 125,000 Silencer Select Negative Control #1 5nmol 4390843 30,000 sirna 40nmol 4390844 90,000 250nmol 4404020 280,000 Silencer Select Negative Control #2 sirna 5nmol 4390846 30,000 40nmol 4390847 90,000 Silencer Select GAPDH Positive Control sirna Silencer Select GAPDH sirna (Hs, Mm, Rn) Custom Select sirna 5nmol 4390849 30,000 40nmol 4390850 90,000 250nmol 4404024 280,000 Custom Select sirna, standard purity 20nmol 4390828 68,000 40nmol 4390829 85,000 Custom Select sirna, HPLC purified 20nmol 4390830 98,000 40nmol 4390831 115,000 sirna sirna 3 sirna sirna Silencer Select Human Kinase sirna Library V4 2,130 710 4397918 Silencer Select Human Phosphatase 894 298 4397919 sirna Library V4 Silencer Select Human GPCR sirna 1,140 380 4397916 Library V4 Silencer Select Human Nuclear 141 47 4397914 Hormone Receptor sirna Library V4 Silencer Select Human Ion Channel 1,014 338 4397915 sirna Library V4 Silencer Select Human Protease 1,482 494 4397917 sirna Library V4 Silencer Select Human Druggable 27,096 9,032 4397920 Genome sirna Library V4-96-well Silencer Select Human Druggable 4,149 1,383 4397921 Genome V4 sirna Ext Set - 96-well Silencer Select Human Extended 31,245 10,415 4397927 Druggable Genome sirna Library V4-96-well Silencer Select Human Druggable 27,096 9,032 4397922 Genome sirna Library V4-384-well Silencer Select Human Druggable 4,149 1,383 4397924 Genome V4 sirna Ext Set - 384-well Silencer Select Human Genome sirna 33,510 11,170 4397923 V4 Extension Set - 384-well Silencer Select Human Genome sirna Library V4-384-well 64,755 21,585 4397926 0.25 nmol 0.5nmol sirna sirna 36 sirna sirna sirna 36 0.1nmol, 0.25 nmol, 1 nmol, 2 nmol 96 384 sirna Inventoried sirna non- Inventoried sirna 34
Ambion In Vivo sirna Ambion In Vivo sirna in vivo RNAisiRNA Silencer Select Silencer Select 33 in vivo RNAi LNA 90% RNAi sirna remaining intact (%) Unmodified sirna: time (h) t 1/2 5 min Ambion In Vivo sirna: t1/2 24 h Ambion In Vivo Pre-designed sirna, 5 nmol 4457298 45,000 Standard Purity Ambion In Vivo Pre-designed sirna, 250 nmol 4457302 300,000 HPLC 1 mol 4457304 480,000 Ambion In Vivo Pre-designed sirna, HPLC In Vivo Ready Ambion In Vivo Custom sirna Standard Purity Ambion In Vivo Custom sirna, HPLC Ambion In Vivo Custom sirna, HPLC In Vivo Ready Ambion In Vivo Custom Designed Standard Purity Ambion In Vivo GAPDH Positive Control sirna Ambion In Vivo GAPDH Positive Control sirna, HPLC In Vivo Ready Ambion In Vivo Factor VII sirna, HPLC In Vivo Ready, 50 nmol Ambion In Vivo Factor VII Positive Control sirna, HPLC In Vivo Ready, 250 nmol Ambion In Vivo Negative Control #1 sirna, 5 nmol Ambion In Vivo Negative Control #1 sirna, HPLC In Vivo Ready, 50 nmol Ambion In Vivo Negative Control #1 sirna, HPLC In Vivo Ready, 250 nmol 250 nmol 4457308 320,000 1 mol 4457310 500,000 5 nmol 4457297 45,000 250 nmol 4457301 300,000 1 mol 4457303 480,000 250 nmol 4457307 320,000 1 mol 4457309 500,000 5 nmol 4457296 45,000 5 nmol 4457288 38,000 50 nmol 4459407 120,000 250 nmol 4457291 320,000 50 nmol 4459408 120,000 250 nmol 4457292 320,000 5 nmol 4457287 38,000 50 nmol 4459405 120,000 250 nmol 4457289 320,000 24. sirna 100 8 Ambion In Vivo sirna 8 sirna 90% sirna HPLC TaqMan sirna Assays TaqMan sirna Assays Silencer Select sirna in vivo sirna TaqMan Assays sirna shrna TaqMan sirna Assays, Made-to-Order, S 150 4440878 39,000 TaqMan sirna Assays, Made-to-Order, M 750 4440879 78,800 TaqMan sirna Assays, Made-to-Order, L 2,900 4440880 146,500 *20µL 35
RNA sirna mirna Lipofectamine RNAiMAX Lipofectamine RNAiMAXsiRNA mirna Silencer Select sirna 33 mirvana mirna mimics & inhibitors 43 sirna Invivofectamine 2.0 Invivofectamine 2.0 sirna mirna in vivo sirna 70% mrna 3mg/kg 3A. mrna p53 % remaining expression 120% 100% 80% 60% 40% 20% mrna 0 7mg/kg 7mg/kg 5mg/kg 3mg/kg 1mg/kg p53 25. Lipofectamine RNAiMAX Lipofectamine RNAiMAX 0.1 1.0 µl RNAiMAXp53 CTRL FVII sirna complexed with Invivofectamine 2.0 3B. % remaining expression 120% 100% 80% 60% 40% 20% 0 7mg/kg 7mg/kg 5mg/kg 3mg/kg 1mg/kg CTRL FVII sirna complexed with Invivofectamine 2.0 3C. Protein 120% p53, relative to nontransfected 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0 10 nm + Lipofectamine RNAiMAX Scrambled control 10 nm + B 10 nm + T p53 sirna 10 nm + Q 1 nm + Lipofectamine RNAiMAX 1 nm + B 1 nm + T 1 nm + Q Nontransfected 26. Lipofectamine RNAiMAX Lipofectamine RNAiMAX 0.1 ml 13778-100 8,900 0.3 ml 13778-030 26,000 0.75 ml 13778-075 63,000 1.5 ml 13778-150 89,500 15 ml 13778-500 735,000 activity % remaining FVII 100% 80% 60% 40% 20% 0 CTRL-7mg/kg No treatment FVII sirna- 3mg/kg FVII sirna- 5mg/kg FVII sirna- 7mg/kg 3mg/kg 5mg/kg 7mg/kg Day 2 Day 9 Day 15 Day 22 Day 29 Days after injection 27. Invivofectamine 2.0 Reagent Ambion In Vivo sirna 3A Invivofectamine 2.0 Reagent Factor VII sirna mrna 95% 1 3 5 7 mg/kg TaqMan 348 Factor VII 95% 3C Factor VII sirna 3 5 7 mg/kg 2 9 15 22 29 FVII Invivofectamine 2.0 Reagent Starter Kit* 1mL 1377501 140,000 Invivofectamine 2.0 Reagent 5mL 1377505 520,000 *Starter Kit sirna Ambion In Vivo Factor VII sirna 50nmol 36
microrna microrna microrna mirna pol precursor mirna mirna 21-23nt mature mirna mirna non-coding RNA mirna mirna mrna 3'UTR mrna mirna Human Precursor mirna Mature mirna 28. mirna (Mature mirna) mirna mirna mirna mirbase www.mirbase.org 29. mirna 8. mirna mirna mirna mirna mirvana mirna Isolation Kit mirvana PARIS Kit TaqMan mirna ABC Purlfication Kit TaqMan MicroRNA Cells-to-CT Kit TaqMan Array MicroRNA Cards TaqMan Universal PCR Master Mix II Megaplex Primer Pools TaqMan MicroRNA RT Kit TaqMan MicroRNA Assays TaqMan Universal PCR Master Mix II TaqMan MicroRNA RT Kit mirvana mirna mimic mirvana mirna inhibitor Lipofectamine RNAiMAX TaqMan Gene Expression Cells-to-CT Kit TaqMan Gene Expression Assays 37
microrna mirvana mirna Isolation Kit RNA RNA microrna mirna small RNA mirvana mirna Isolation Kit mirna RNA mirna Total RNA 250mg Total RNA 30 mirvana mirna Isolation Kit 40 AM1560 50,300 mirvana mirna Isolation Kit without Phenol 40 AM1561 44,000 mirvana PARIS Kit small RNA RNA 2 1 Small RNA RNA mirna 30 small RNA RNA mrna mirna sirna 10 2 10 7 100mg 200 small RNA RNA TaqMan mirna ABC Anti-miRNA Bead Capture Purification Kit mirna 75 Human Panel A B 754 mirna mirna Anti-miRNA oligos Dynabeads mirna mirna 10 L 50 L RT-qPCR TRIzol 75 mirna Avg. C t 40.00 35.00 30.00 25.00 20.00 15.00 10.00 cel-mir-39 expression profile serum sample (50 μl) hsa-mir-16 hsa-let-7a Assay ID 30. TaqMan mirna ABC Purification Ki 50 L hsa-mir-150 hsa-let-7c hsa-mir-135b mirvana PARIS Kit 40 AM1556 57,800 31. ABC Purification Kit TaqMan mirna ABC Purification 40 4473087 60,000 Kit-Human Panel A TaqMan mirna ABC Purification Kit-Human Panel B 40 4473088 60,000 38
microrna TaqMan MicroRNA RT Kit TaqMan MicroRNA RT Kit TaqMan MicroRNA Assays 40 TaqMan MicroRNA Assays RT TaqMan MicroRNA RT Kit 200 4366596 24,300 1,000 4366597 72,800 Megaplex Primer Pools Megaplex RT Primers stem-looped Sanger mirbase mirna mirna RNA Megaplex Primer Pools TaqMan MicroRNA Assays TaqMan MicroRNA Arrays Megaplex PreAmp Primers 1ng RNA Megaplex RT Primers Pool (Rodent) Megaplex RT Primers, Rodent Pool A 50 4399970 86,900 Megaplex RT Primers, Rodent Pool B 50 4399972 86,900 Megaplex RT Primers, Rodent Pool Set 50 4400926 100,000 RT Primers Pools A&B (4399970 & 4399972) Megaplex RT Primers, Rodent Pool B v3.0 50 4444292 86,900 Megaplex RT Primers, Rodent Pool Set v3.0 RT Primers Pools A&B (4399970 & 4444292) 50 4444746 100,000 Megaplex PreAmp Primers Pool (Rodent) Megaplex PreAmp Primers, Rodent Pool A 50 4399203 86,900 PreAmp Megaplex PreAmp Primers, Rodent Pool B 50 4399237 86,900 PreAmp Megaplex PreAmp Primers, Rodent Pool Set 50 4400925 100,000 PreAmp Primers Pools A&B (4399203 & 4399237) Megaplex PreAmp Primers, Rodent Pool B v3.0 50 4444308 86,900 PreAmp Megaplex PreAmp Primers, Rodent Pool Set v3.0 PreAmp Primers Pools A&B (4399203 & 4444308) 50 4444747 100,000 Megaplex RT and PreAmp Primers Package (Rodent) Megaplex Primer Pools, Rodent Pools A 50 4401090 121,200 RT Primers (4399970) PreAmp Primers (4399203) Megaplex Primer Pools, Rodent Pools B 50 4401011 121,200 RT Primers (4399972) PreAmp Primers (4399237) Megaplex Primer Pools, Rodent Pools Set 50 4401012 137,400 RT Primers Pools A&B (4400926) PreAmp Primers Pools A&B (4400925) Megaplex Primer Pools, Rodent Pools B v3.0 50 4444752 121,200 RT Primers (4444292) PreAmp Primers (4444308) Megaplex Primer Pools, Rodent Pools Set v3.0 RT Primers Pools A&B (4444746) PreAmp Primers Pools A&B (4444747) 50 4444766 137,400 Megaplex RT Primers Pool (Human) Megaplex RT Primers, Human Pool A 50 4399966 86,900 Megaplex RT Primers, Human Pool B Megaplex RT Primers, Human Pool Set RT Primers Pools A&B (4399966 & 4399968) Megaplex RT Primers, Human Pool B v3.0 Megaplex RT Primers, Human Pool Set v3.0 RT Primers Pools A&B (4399966 & 4444281) 50 4399968 86,900 50 4400928 100,000 50 4444281 86,900 50 4444745 100,000 Megaplex PreAmp Primers Pool (Human) Megaplex PreAmp Primers, Human Pool A 50 4399233 86,900 PreAmp Megaplex PreAmp Primers, Human Pool B PreAmp Megaplex PreAmp Primers, Human Pool Set PreAmp Primers Pools A&B (4399233 & 4399201) Megaplex PreAmp Primers, Human Pool B v3.0 PreAmp Megaplex PreAmp Primers, Human Pool Set v3.0 PreAmp Primers Pools A&B (4399233 & 4444303) 50 4399201 86,900 50 4400927 100,000 50 4444303 86,900 50 4444748 100,000 Megaplex RT and PreAmp Primers Package (Human) Megaplex Primer Pools, Human Pools A RT Primers (4399966) PreAmp Primers (4399233) 50 4401009 121,200 Megaplex Primer Pools, Human Pools B RT Primers (4399968) PreAmp Primers (4399201) Megaplex Primer Pools, Human Pools Set RT Primers Pools A&B (4400928) PreAmp Primers Pools A&B (4400927) Megaplex Primer Pools, Human Pools B v3.0 RT Primers (4444281) PreAmp Primers (4444303) Megaplex Primer Pools, Human Pools Set v3.0 RT Primers Pools A&B (4444745) PreAmp Primers Pools A&B (4444748) 50 4401010 121,200 50 4401091 137,400 50 4444749 121,200 50 4444750 137,400 TaqMan MicroRNA Arrays A B Megaplex Primer Pools 39
microrna TaqMan MicroRNA Assays TaqMan MicroRNA Assays PCR microrna mirna TaqMan MicroRNA Assays mirna mirna mirna mirna Assays Synthetic mirna Target Relative Detection (%) No. of base differences TaqMan mirnamirna PCR 3 1-10ng RNA 7 TaqMan MicroRNA Assays TaqMan MicroRNA Assays mirna loop mirna RT mirna mirna 5' TaqMan PCR mirna Mature mirna Looped RT primer 33. 1 TaqMan MicroRNA Assays 1let-7a let-7a let-7c 3.7 TaqMan MicroRNA Assays, 50 4427975 29,800 Inventoried* mirna RT primer 5X reaction mix (50 / 15 µl ) mirna TaqMan Assay 20X reaction mix (150 / 20 µl ) 150 TaqMan MicroRNA Assays, Made-to-Order, Small mirna RT primer 5X reaction mix (50 / 15 µl ) mirna TaqMan Assay 20X reaction mix (150 / 20 µl ) TaqMan MicroRNA Assays, Made-to-Order, Medium TaqMan MicroRNA Assays, Made-to-Order, Large 50 150 750 750 2,900 2,900 4440886 39,000 4440887 78,800 4440888 146,500 * Arabidopsis thaliana Caenorhabditis elegans Drosophila melanogaster Made-to-Order Sanger Release 16 mirna 2011 2 Custom TaqMan Small RNA Assays Forward primer TaqMan probe Reverse primer 32. TaqMan MicroRNA Assays TaqMan MicroRNA Assays mirna mirnas Custom TaqMan Small RNA Assays, 50 4398987 50,000 150 Custom TaqMan Small RNA Assays, 750 4398988 121,200 2,250 Custom TaqMan Small RNA Assays, 2,900 4398989 256,600 8,700 *20µL 40
TaqMan Array MicroRNA Card TaqMan Array MicroRNA Card TaqMan 384 TaqMan 8 381 mirna 1 Megaplex RT Primers 39 TaqMan MicroRNA Array v2.0 / v3.0 Megaplex PreAmp Primers TaqMan MicroRNA Arrays v2.0 / v3.0 Megaplex Primer Pools Sanger mirbase 5 TaqMan Array Applied Biosystems 7900HT ViiA 7 QuantStudio TaqMan Array mature mirna Human TaqMan Array Human MicroRNA A+B Cards Set v2.0 A array (4398965) + B Array (4398966) TaqMan Array Human MicroRNA A Cards v2.0 TaqMan Array Human MicroRNA B Cards v2.0 TaqMan Array Human MicroRNA A+B Cards Set v3.0 A array (4398965) + B Array (4444910) TaqMan Array Human MicroRNA B Cards v3.0 Rodent 8 4400238 260,600 4 4 4398965 202,000 4 4398966 202,000 8 4444913 260,600 4 4 4444910 202,000 TaqMan Array Rodent MicroRNA A+B Cards Setv2.0 A array (4398967) + B Array (4398968) TaqMan Array Rodent MicroRNA A Cards v2.0 TaqMan Array Rodent MicroRNA B Cards v2.0 TaqMan Array Rodent MicroRNA A+B Cards Set v3.0 A array (4398967) + B Array (4444899) TaqMan Array Rodent MicroRNA B Cards v3.0 8 4400239 260,600 4 4 4398967 202,000 4 4398968 202,000 8 4444909 260,600 4 4 4444899 202,000 mirvana mirna Isolation Kit TaqMan mirna ABC Purification Kit Megaplex RT Primers & TaqMan MicroRNA Reverse Transcription Kit Megaplex PreAmp Primers & TaqMan PreAmp Master Mix Diluted RT product & TaqMan Universal PCR Master Mix II Applied Biosystems QuantStudio 12K Flex ViiA 7 / 7900 HT Fast PCR RT-PCR Expression Suite Software 34. mirna 41
Non-coding RNA TaqMan Pri-miRNA Assays mirna TaqMan Pri-miRNA Assays TaqMan Pri-miRNA Assays microrna mirna primary microrna Pri-miRNA microrna Pri-miRNA 1 microrna Drosha mirna mirna stem-loop Dicer RNA Induced Silencing Complex RISC mirna 35. microrna 37. hsa-mir-26 mir-26a-1 mir-26a-2 Pri-miRNA Stemloop Pri-miR-26a-2 TaqMan Pri-miRNA Assay hsa-mir-26a-2 TaqMan Non-coding RNA Assays TaqMan Non-coding RNA Assays TaqMan Non-coding RNA Assays non-cording TaqMan Gene Expression AssaysTaqMan Non-coding RNA TaqMan Non-coding RNA Assays TaqMan PreAmp Master Mix Pre-Amplification 36. TaqMan Pri-miRNA Assays Sanger mirbase Stem-loop 500 Pri-miRNA Sanger mirbase sequence repository stem-loop mirna TaqMan Pri-miRNA Assay TaqMan MicroRNA AssaymiRNA TaqMan Non-coding RNA Assays, S 360 4426961 39,000 TaqMan Non-coding RNA Assays, M 750 4426962 78,800 TaqMan Non-coding RNA Assays, L 2,900 4426963 146,500 *20µL TaqMan Pri-miRNA Assays, S 360 4427012 39,000 TaqMan Pri-miRNA Assays, M 750 4427013 78,800 TaqMan Pri-miRNA Assays, L 2,900 4427014 146,500 *20µL 42
microrna mirvana mirna mimic & inhibitors mirna mirna mirbase in vivo in vivo ready mirvana mirna mimic mirvana mirna mimic mirna mirna mirna mirna 2 mirna mirvana mirna mimic 5 nmol 4464066 58,000 20 nmol 4464067 178,000 mirvana mirna mimic, in vivo Ready 250 nmol 4464070 510,000 Customer-Defined mirvana mirna mimic Customer-Defined mirvana mirna mimic, in vivo Ready mirvana mirna mimic Negative Control #1 mirvana mirna mimic Negative Control #1, in vivo Ready mirvana mirna mimic mir-1 Positive Control mirvana mirna mimic mir-1 Positive Control, in vivo Ready mirvana mirna mimic mirbase v 19 2013 3 5 nmol 4464068 98,000 20 nmol 4464069 180,000 250 nmol 4464071 510,000 5 nmol 4464058 57,000 20 nmol 4464059 174,000 50 nmol 4464060 250,000 250 nmol 4464061 500,000 5 nmol 4464062 57,000 20 nmol 4464063 174,000 50 nmol 4464064 250,000 250 nmol 4464065 500,000 0.25 nmol 4464074 each 1 nmol 4464075 each mirvana mirna inhibitor mirvana mirna inhibitormirna mirna mirna 1 mirvana mirna inhibitor 5 nmol 4464084 58,000 20 nmol 4464085 178,000 mirvana mirna inhibitor, in vivo 250 nmol 4464088 510,000 Ready Customer-Defined mirvana mirna 5 nmol 4464086 98,000 inhibitor 20 nmol 4464087 180,000 Customer-Defined mirvana mirna 250 nmol 4464089 510,000 inhibitor, in vivo ready mirvana mirna inhibitor Negative 5 nmol 4464076 57,000 Control #1 20 nmol 4464077 174,000 mirvana mirna inhibitor Negative 50 nmol 4464078 250,000 Control #1, in vivo Ready 250 nmol 4464079 500,000 mirvana mirna inhibitor let-7 5 nmol 4464080 57,000 Positive Control 20 nmol 4464081 174,000 mirvana mirna inhibitor 0.25 nmol 4464092 mirbase v 19 each 1 nmol 4464093 each 2013 3 Pre-miR mirna Precursor Molecule Pre-miR mirna Precursor Starter Kit 1 AM1540 94,000 Pre-miR mirna Precursors 5nmol AM17100 55,000 Molecule 20nmol AM17101 204,000 4 5nmol Customer-Defined Pre-miR mirna Precursor 20nmol 4 5nmol AM17103 204,000 Pre-miR Negative Control #1 5nmol AM17110 55,000 Pre-miR Negative Control #2 5nmol AM17111 55,000 Cy3 Pre-miR Negative Control 5nmol AM17120 62,000 FAM Pre-miR Negative Control 5nmol AM17121 57,000 Pre-miR hsa-mir-1 mirna Precursor 5nmol AM17150 55,000 Anti-miR mirna Inhibitor Anti-miR mirna Inhibitor 5nmol AM17000 55,000 20nmol AM17001 204,000 4 5nmol Customer Defined Anti-miR mirna Inhibitor 20nmol 4 5nmol AM17003 204,000 Anti-miR Negative Control #1 5nmol AM17010 55,000 Cy3 Anti-miR Negative Control 5nmol AM17011 60,000 FAM Anti-miR Negative Control 5nmol AM17012 57,000 mirna 36 Lipofectamine RNAiMAX Invivofectamine 2.0 43
SNP TaqMan SNP 2 TaqMan TaqMan 5' VIC dye FAM dye 3' Q PCR DNA VIC dye FAM dye TaqMan SNP Genotyping Assay 100 DNA 38. TaqMan SNP 39. Allele1 TaqMan VIC Allele2 TaqMan FAM VIC FAM Allele1 Allele2 Allele1 2 44
SNP TaqMan Pre-Designed SNP Genotyping Assays TaqMan SNP Genotyping Assays SNP SNP 450 1 SNP ID SNP csnp 7 SNP 16 SNP 350 SNP 2600 Human TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Small scale 300 1,500 4351379 53,000 TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Medium scale TaqMan Pre-Designed SNP Genotyping Assays, Human, non-inventried Large scale * 25µL 5µL non-human 1,000 5,000 2,400 12,000 4351376 96,000 4351374 185,900 TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Small scale 300 1,500 4351384 53,000 TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Medium scale TaqMan Pre-Designed SNP Genotyping Assays Non-Human non-inventoried Large scale * 25µL 5µL 1,000 5,000 2,400 12,000 4351382 96,000 4351380 185,900 Custom TaqMan SNP Genotyping Assays Custom TaqMan SNP Genotyping Assays SNP SNP SNP SNP SNP 6 MNP SNP Human Custom TaqMan SNP Genotyping 300 4331349 58,600 Assays, S-scale, Human 1,500 Custom TaqMan SNP Genotyping 1,000 4332072 111,100 Assays, M-scale, Human 5,000 Custom TaqMan SNP Genotyping Assays, L-scale, Human 2,400 12,000 4332073 222,200 * 25µL 5µL non-human Custom TaqMan SNP Genotyping 300 4332077 58,600 Assays, S-scale, non-human 1,500 Custom TaqMan SNP Genotyping 1,000 4332075 111,100 Assays, M-scale, non-human 5,000 Custom TaqMan SNP Genotyping Assays, L-scale, non-human 2,400 12,000 4332076 222,200 * 25µL 5µL TaqMan Drug Metabolism Genotyping Assays 220 2,000 TaqMan Drug Metabolism Genotyping 150 4362691 59,600 Assays 750 * 25µL 5µL 45
SNP SNP TaqMan Genotyping Master Mix TaqMan Genotyping Master Mix PCR GC AT TaqMan Universal Master Mix GC AT 72 Fast SNP TaqMan GTXpress Master Mix TaqMan GTXpress Master Mix Fast PCR SNP SNP TaqMan PCR 50 PCR 50 Tracking Dye 9. PCR 40. TaqMan Genotyping Master Mix GC-rich TaqMan Genotyping Master Mix 94 gdna TaqMan SNP Assay C 12050942_10 PCR GeneAmp PCR System 9700 7900HT PCR GC-rich Catjespin D rs12214 dbsnp TaqMan Genotyping Master Mix TaqMan Genotyping Master Mix, 80 4371353 14,000 1mL 400 TaqMan Genotyping Master Mix, 10mL 800 4,000 4371355 115,000 TaqMan Genotyping Master Mix, 10mL 2 TaqMan Genotyping Master Mix, 50mL TaqMan Genotyping Master Mix, 50mL 2 TaqMan Genotyping Master Mix TaqMan Genotyping Master Mix * 25µL 5µL 1,600 8,000 4,000 20,000 8,000 40,000 4381656 220,000 4371357 500,000 4381657 970,000 1 4371131 1 4371130 TaqMan GTXpress Master Mix TaqMan GTXpress Master Mix, 1mL 80 4403311 14,000 200 400 TaqMan GTXpress Master Mix, 10mL 800 4401892 115,000 2,000 4,000 TaqMan GTXpress Master Mix, 50mL 4,000 4401890 500,000 10,000 20,000 TaqMan GTXpress Master Mix, 100mL 2 50mL 8,000 20,000 40,000 4401857 970,000 *PCR 96 Standard Block,0.2µL 25µL 96 Fast Block,0.1µL 10µL 384 5µL 46
SNP TaqMan Sample-to-SNP Kit TaqMan Sample-to-SNP Kit DNA TaqMan SNP 1 DNAFast PCR TaqMan GTXpress Master Mix DNA 5 PCR 50 SNP 1 / FTA / / / Buccal swab / Rat Mouse tail / Leaf punch / / FFPE DNA 95 3 DNA PCR Fast PCR TaqMan GTXpress Master Mix PCR 50 41. TaqMan Sample-to-SNP Kit A. Buccal swab, B. Mouse tail, C. Corn leaf, D. FFPE TaqMan Sample-to-SNP Kit DNA 100 4403313 34,000 5mL sample prep, 1mL PCR PCR 80 TaqMan Sample-to-SNP Kit 20mL sample prep, 10mL PCR TaqMan Sample-to-SNP Kit 20mL sample prep, 50mL PCR TaqMan Sample-to-SNP Kit 200mL sample prep, 10mL PCR * PCR 25µL DNA 400 PCR 800 DNA 400 PCR 4,000 DNA 4,000 PCR 800 4403083 180,000 4403087 550,000 4403081 560,000 42. TaqMan Sample-to-SNP Kit SNP www.lifetechnologies.com/taqmanjp SNP / 47
TaqMan Mutation Detection Assays TaqMan Mutation Detection Assays Competitive Allele-Specific TaqMan PCR castpcr MGB 0.1 1 3 1 10. TaqMan Mutation Detection Assay 11. Reference Assay Mutant Assay Mutant Allele Assay EGFR_exlqdels_mu* 19 MGB ASP = ASB = MGB LST = TaqMan LSP = EGFR exon19 deletion Wild type Assay Mutant Assay EGFR Reference Assay EGFR Exon19 Deletion Assay Wild type Allele Assay MGB ASP = ASB = MGB LST = TaqMan LSP = Mutation Detector TaqMan Mutation Detection Assay Gene Reference Assay FP = RP = LST = TaqMan ASP: LST: TaqMan LSP: MGB: ASB: MGB FP: RP: TaqMan Mutation Detection Assays 46 778 ** 150µL 75 4465804 80,800 TaqMan EGFR Exon 19 Deletions Assay 1 EGFR 19 1 ** 150µL 75 4465805 80,800 TaqMan Mutation Detection Reference Assays 46 ** 150µL 75 4465807 50,000 TaqMan Mutation Detection IPC Reagent Kit 1 kit 500 4467538 28,300 *20µL ** 2012 11 www.lifetechnologies.com TaqMan Mutation Detection Assays 48
TaqMan Copy Number Assays Copy Number Variations, CNVs CNVs CNVs TaqMan Copy Number Assays CNVs 160 TaqMan CNVs TaqMan Copy Number Assays TaqMan Copy Number Reference Assays PCR 3 4 PCR DNA 3 4 160 Copy Number 4 TaqMan Copy Number Assays, 360 assays 4400291 45,000 Small 720 assays TaqMan Copy Number Assays, 750 assays 4400292 85,900 Medium 1,500 assays TaqMan Copy Number Assays, Large 2,900 assays 5,800 assays 4400293 161,600 * 20µL 96 10µL 384 Custom TaqMan Copy Number Assays Custom TaqMan Copy Number Assays TaqMan Copy Number Assays CNVs GeneAssist Copy Number Assay Tool CNVs Custom TaqMan Copy Number Assays, 360 assays 4400294 50,000 Small 720 assays Custom TaqMan Copy Number Assays, 750 assays 4400295 98,000 Medium 1,500 assays Custom TaqMan Copy Number Assays, Large 2,900 assays 5,800 assays 4400296 181,800 * 20µL 96 10µL 384 CopyCaller TaqMan Copy Number Assays CopyCallerTM www.lifetechnologies.com/copycaller TaqMan Copy Number Reference Assays TaqMan Copy Number Reference Assays TaqMan Copy Number Assays Custom TaqMan Copy Number Assays RNase P Ribonuclease P RNA component H1 (H1RNA) gene (RPPH1) on chromosome 14, cytoband 14q11.2TERT RNase P telomerase reverse transriptase (TERT) gene located on chromosome 5, cytoband 5p15.33 Human TaqMan Copy Number Reference Assay, 750 assays 4403326 18,200 RNase P 1,500 assays 3,000 assays 6,000 assays 4403328 51,500 TaqMan Copy Number Reference Assay, TERT 750 assays 1,500 assays 3,000 assays 6,000 assays 4403316 18,200 4403315 51,500 Mouse * TaqMan Copy Number Reference Assay, 750 assays 4458366 18,200 TFRC 1,500 assays 3,000 assays 6,000 assays 4458367 51,500 TaqMan Copy Number Reference Assay, TERT 750 assays 1,500 assays 3,000 assays 6,000 assays 4458368 18,200 4458369 51,500 * 20µL 96 10µL 384 Custom Plus TaqMan Copy Number Assays Custom Plus TaqMan Copy Number Assays Custom TaqMan Copy Number Assays SNP TaqMan TaqMan Copy Number Assays * Custom Plus TaqMan Copy Number 360assays 4442487 50,000 Assays, Small 720assays Custom Plus TaqMan Copy Number 750assays 4442520 98,000 Assays, Medium 1,500assays Custom Plus TaqMan Copy Number Assays, Large 2,900assays 5,800assays 4442488 181,800 * 20µL 96 10µL 384 49
High Resolution Melt HRM High Resolution Melting HRM PCR PCR PCR MeltDoctor HRM MeltDoctor HRM 1 * 2 PCR 3 PCR 4 HRM 5 HRM PCR PCR PCR 3 5 PCR *Only required once every six months HRM PCR 43. HRM MeltDoctor HRM High Resolution Melting (HRM) PCR MeltDoctor HRM SYTO 9 DNA Tm PCR dutp dntp PCR HRM 50
MeltDoctor HRM HRM AmpliTaq Gold 360 DNA Polymerase PCR MeltDoctor HRM 2 SYTO 9 MeltDoctor HRM MeltDoctor HRM HRMPCR MeltDoctor HRM 1 500units 4425557 48,000 AmpliTaq Gold 360 DNA Polymerase GeneAmp dntp Blend MeltDoctor HRM Dye MeltDoctor HRM 500 4415440 39,000 5 ml MeltDoctor HRM 2,500 4415452 180,000 5 5 ml MeltDoctor HRM 5,000 4415450 340,000 10 5 ml MeltDoctor HRM 5,000 4409535 320,000 50 ml *20µL MeltDoctor HRM MeltDoctor HRM 3 AA, AB, BB 3 DNA MeltDoctor HRM 1 450 4410126 40,000 MeltDoctor HRM MeltDoctor HRM 96 Fast 384 2 MeltDoctor HRM DNA 44. HRM 3 HeLa, Raji, and Jurkat HRM p53 4152bp PCR 3 HRM GC, GG, CC HRM MeltDoctor HRM Applied Biosystems 7500 Fast PCR Applied Biosystems HRM Software v2.0 HRM PCR BigDye Terminator v1.1 Cycle Sequencing Kit Applied Biosystems 3130 MeltDoctor HRM 1 4425618 50,000 96 Fast MeltDoctor HRM 384 1 4425559 50,000 MeltDoctor HRM 20 1mL 4425562 40,000 HRM HRM HRM PCR www.lifetechnologies.com/hrm 51
TaqMan Protein Assays 3 5 1 2 Build Your Assay 90 min Prepare Sample 30 min Binding 60 min - TaqMan Protein Assay Open Kit Protein Quant Sample Lysis Kit Protein Expression Sample Lysis Kit Ambion PARIS Kit TaqMan Protein Assay TaqMan Protein Assay Open Kit TaqMan Protein Assay Kit Ligation/ Inactivation 25 min TaqMan Fast Real-Time PCR 40 min Data Analysis DNA TaqMan Protein Assay Core Reagents Kit with Master Mix Universal TaqMan Assay PCR TaqMan PCR mrna mirna ProteinAssist Software v1.0 45. TaqMan Protein Assays 52
TaqMan Protein Assays TaqMan Protein Assays TaqMan 5 mrna mirna 500 4 TaqMan Protein Expression Core Reagents Kit with Master Mix TaqMan PCR TaqMan Protein Assays Kit TaqMan Protein Assays Open Kit TaqMan Protein Assay Core Reagent Base Kit DNA Ligase, Protease, Ligase PCR / TaqMan Protein Assays Fast Master Mix ph PCR TaqMan Protein Assays Kit (hesc ) hicam1 hcstb TaqMan Protein Assay Kit hcstb 100 4405465 56,600 TaqMan Protein Assay Kit hicam1 100 4405471 56,600 TaqMan Protein Assay Open Kit TaqMan Protein Assay 2 TaqMan PCR mrna mirna TaqMan Protein Assays Open Kit TaqMan Protein Assays Oligo Probe Kit TaqMan Protein Assays Buffer Kit 4,000 4453745 129,300 TaqMan Protein Assays Oligo Probe Kit 4,000 4448549 54,600 5'Prox-Oligo 200nM, 50µL 3'Prox-Oligo 200nM, 50µL TaqMan Protein Assays Buffer Kit Antibody Dilution Buffer, 1mL Assay Probe Strage Buffer, 1mL Assay Probe Dilution Buffer, 7mL Lysate Dilution Buffer, 25mL 4,000 4448571 83,900 TaqMan Protein Expression Core 100 4405501 58,600 Reagents Kit with Master Mix TaqMan Protein Expression Core Reagents Base Kit 4405460 TaqMan Protein Assays Fast Master Mix 4400088 TaqMan Protein Expression Core Reagents Kit with Master Mix TaqMan ProteinExpression Core Reagents Base Kit 4448592 TaqMan Protein Assays Fast Master Mix 4448616 500 4448591 171,700 TaqMan Protein Assays Core Reagents 100 4405460 48,500 Base Kit 500 4448592 139,400 TaqMan Protein Assays Fast Master Mix 100 4400088 20,200 500 4448616 56,600 53
Protein Expression Sample Preparation Kit TaqMan Protein Assay Kit TaqMan Protein Assay Open Kit 10,000 50,000 Protein Quant Sample Lysis Kit 50,000 Protein Expression Lysate Control Kits TaqMan Protein Assays Protein Expression Lysate Control Kit (Raji) hcstb hicam1 Protein Expression Lysate Control Kit 100 4405448 60,600 Raji ProteinAssist TaqMan Protein Assays ProteinAssist www.appliedbiosystems.com/proteinassist 12. Protein Expression Sample Preparation Kit Protein Quant Sample Lysis Kit 10,000 50,000 50,000 Lysis Yes No TaqMan Protein Assays 500 cells/µl 2,500 cells/µl TaqMan Protein Assay 1:2 1:10 Protein Expression Sample Prep Kit cell counting 1,000 4405443 72,800 Protein Quant Sample Lysis Kit cell counting or total protein quant 25 ml 4448536 28,300 54
PCR Protein Thermal Shift PCR PCR 1 10 * PCR 48 96 384 * 1 Protein Thermal Shift 10 4466037 480,000 1.0 Protein Thermal Shift 1 4466038 240,000 Protein Thermal Shift Dye Kit 2,000 4461146 19,000 Protein Thermal Shift 2,000 + 4462263 44,000 Starter Kit 100 100,000 Protein 80,000 Protein + Ligand d /dt 60,000 40,000 20,000 0 15,000 10,000 5,000 0-5,000 30.0 32.5 35.0 37.5 40.0 42.5 45.0 47.5 50.0 52.5 55.0 ~7oC Tm Difference 30.0 32.5 35.0 37.5 40.0 42.5 45.0 47.5 50.0 52.5 55.0 StepOne /StepOnePlus 7500 / 7500 Fast ViiA 7 / QuantStudio 12K Flex PCR StepOne / StepOnePlus v2.2 7500 / 7500 Fast v2.0.4 ViiA 7 v1.0 QuantStudio 12K Flex 46. Tm 30 PCR 47. Protein Thermal Shift TM 55
13. 384-Well Clear Optical Reaction Plate ( 4309849) 96-Well Reaction Plate ( N8010560 / 4306737) + + Adhesive Film ( 4311971 / 4360954) Adhesive Film ( 4311971 / 4360954) QuantStudio 12K Flex PCR Applied Biosystems ViiA 7 PCR QuantStudio 12K Flex ViiA 7 96-Well Reaction Plate ( N8010560 / 4306737) Optical Tubes ( N8010933) Fast 8-Tube Strip, 0.1mL ( 4358293) Fast 96-Well Reaction Plate ( 4346907 / 4346906) + or Optical 8-Tube Strip and Fast Reaction Tube with Cap, 0.1mL ( 4358297) + + + Optical Caps (Flat Cap) ( 4323032) Tray / Retainer Set ( 4381850) ( 4316567) + + 96-Well Tray for VeriFlex TM Blocks ( 4379983) 96-Well Tray for VeriFlex TM Blocks ( 4379983) Adhesive Film ( 4311971 / 4360954) + Optical Caps (Flat Cap) ( 4323032) Optical 8-Cap Strip ( 4323032) 384-Well Clear Optical Reaction Plate ( 4309849) + Adhesive Film ( 4311971 / 4360954) Applied Biosystems 7900HT Fast PCR 7900HT 96-Well Reaction Plate ( N8010560 / 4306737) 96-Well Reaction Plate ( N8010560 / 4306737) Fast 96-Well Reaction Plate ( 4346907 / 4346906) + + + Adhesive Film ( 4311971 / 4360954) Optical 8-Cap Strip ( 4323032) Adhesive Film ( 4311971 / 4360954) + Compression Pad ( 4312639) 7500 Fast Fast 96-Well Reaction Plate ( 4346907 / 4346906) Fast 8-Tube Strip, 0.1mL ( 4358293) + + Adhesive Film ( 4311971 / 4360954) Optical 8-Cap Strip ( 4323032) Applied Biosystems 7500 Fast / 7500 PCR 7500 96-Well Reaction Plate ( N8010560 / 4306737) + Adhesive Film ( 4311971 / 4360954) 7300 Optical Tubes or Optical 8-Tube Strip Optical 8-Cap Strip Optical 8-Tube Strip ( N8010933) and ( 4316567) + ( 4323032) StepOne Plus Fast 96-Well Reaction Plate ( 4346907 / 4346906) Fast 8-Tube Strip, 0.1mL ( 4358293) Fast Reaction Tube with Cap, 0.1mL ( 4358297) + + + Adhesive Film ( 4311971 / 4360954) 96-Well Tray for VeriFlex TM Blocks ( 4379983) 96-Well Tray for VeriFlex TM Blocks ( 4379983) + Optical 8-Cap Strip ( 4323032) StepOnePlus TM /StepOne TM PCR StepOne Fast Optical 48-Well Reaction Plate ( 4375816) Fast 8-Tube Strip, 0.1mL ( 4358293) + + 48-Well Optical Adhesive Film ( 4375928 / 4375323) Fast 48-Well Tray ( 4375282) + Optical 8-Cap Strip ( 4323032) Fast Reaction Tube with Cap, 0.1mL ( 4358297) + Fast 48-Well Tray ( 4375282) ABI PRISM 7000 7700 5700 7000 96-Well Reaction Plate ( N8010560 / 4306737) 96-Well Reaction Plate ( N8010560 / 4306737) Optical Tubes ( N8010933) or Optical 8-Tube Strip and ( 4316567) + + + Adhesive Film ( 4311971 / 4360954) Optical 8-Cap Strip ( 4323032) Tray / Retainer Set ( 403081) + + Compression Pad ( 4312639) Optical 8-Cap Strip ( 4323032) 56
Fast 96-Well 7900HT Fast 96 / 7500 Fast / StepOnePlus ViiA 7 / QuantStudio Fast 96 MicroAmp Fast 96-Well Reaction 10 4346907 6,200 Plate 0.1mL MicroAmp Fast Optical 96-Well 20 4346906 16,000 Reaction Plate with Barcode 0.1mL 200 4366932 145,000 7900HT / 7700 / 7500 / 7500 Fast / 7300 / 7000 / 5700 / StepOnePlus / ViiA 7 / QuantStudio MicroAmp Optical Adhesive Film 25 4360954 10,000 100 4311971 32,500 MicroAmp Adhesive Film Applicator 5 4333183 10,000 5 96-Well 7900HT 96 / 7700 / 7500 / 7300 / 7000 / 5700 ViiA 7 / QuantStudio Standard 96 MicroAmp Optical 96-Well Reaction Plate 10 N8010560 6,000 MicroAmp Optical 96-Well Reaction Plate 20 4306737 15,800 with Barcode MicroAmp Optical 96-Well Reaction Plate 500 4326659 340,000 with Barcode MicroAmp Optical 96-Well Reaction Plate with Barcode 4306737 25 MicroAmp Optical 96-Well Reaction Plate 1 403012 26,000 with Barcode and Optical Caps MicroAmp Optical 96-Well Reaction Plate with Barcode 4306737 1 MicroAmp Optical 8-Cap Strip 4323032 1 96-Well Compression Pad 7900HT* / 7700 / 7000 / 5700 96 Optical Adhesive Film+Compression Pad * Fast 96 384 TaqMan Low Density Array Compression Pad MicroAmp Optical Adhesive Film Kit 1 4313663 8,500 MicroAmp Optical Adhesive Film 20 1 MicroAmp Optical Film Compression Pad 1 7900HT 96 * / 7700 / 7500 / 7500Fast / 7000 / 5700 / StepOnePlus / StepOne / ViiA 7 / QuantStudio * 384 TaqMan Array Optical Cap MicroAmp Optical 8-Cap Strip Flat 300 4323032 15,800 N8010935 384-Well 7900HT 384 / ViiA 7 / QuantStudio 384 MicroAmp Optical 384-Well Reaction 50 4309849 50,000 Plate with Barcode MicroAmp Optical 384-Well Reaction 500 4326270 400,000 Plate with Barcode MicroAmp Optical 384-Well Reaction Plate with Barcode 4309849 10 MicroAmp Optical 384-Well Reaction 1,000 4343814 660,000 Plate with Barcode Optical 384-Well Reaction Plate 1000 Code 128 MicroAmp Optical 384-Well Reaction Plate 1,000 4343370 640,000 Optical 384-Well Reaction Plate 1000 7700 / 7500 / 7000 / 5700 / ViiA 7 / QuantStudio Standard 96 MicroAmp Optical 8-Tube Strip 125 4316567 15,800 0.2mL MicroAmp Optical Tube without Cap 0.2mL 2,000 N8010933 38,000 57
Fast 7500Fast / StepOnePlus / StepOne / ViiA 7 / QuantStudio Fast 96 MicroAmp Fast 8-Tube Strip 125 4358293 18,000 0.1mL & 7700 / 7000 / 5700 MicroAmp 96-Well Tray/Retainer Set 10 403081 12,000 Fast StepOnePlus / StepOne MicroAmp Fast Reaction Tube 1,000 4358297 21,000 with Cap 0.1mL StepOne 48-Well StepOne 48 MicroAmp Fast Optical 48-Well 20 4375816 12,000 Reaction Plate MicroAmp Optical 48-Well Adhesive Film StepOnePlus StepOnePlus 96 25 4375928 9,800 100 4375323 29,000 MicroAmp Fast 48-Well Trays 10 4375282 11,000 MicroAmp 48-Well Base Adaptor 5 4375284 5,800 MicroAmp 96-Well Tray for VeriFlex 10 4379983 8,900 Blocks 96 96 *StepOnePlus / StepOne 4379590 MicroAmp Splash Free 96-Well Base 10 4312063 13,500 MicroAmp 96-Well Support Base 10 4379590* 13,800 Compression Pad 7900HT* / 7700 / 7000 / 5700 96 Optical Adhesive Film Compression Pad * Fast 96 384 TaqMan Low Density Array Compression Pad MicroAmp Optical Film Compression 5 4312639 7,500 Pad ViiA 7 / QuantStudio ViiA 7 / QuantStudio Standard 96 MicroAmp 96-Well Tray/Retainer Set 10 4381850 10,200 for Veriti System ViiA 7 / QuantStudio Fast 96 MicroAmp 96-Well Tray for VeriFlex 10 4379983 8,900 Blocks LAMP for GeneAmp 5700 and 1 4309224 17,000 ABI PRISM 7000 System LAMP for Applied Biosystems 7300/7500 / ViiA 7 System 1 4345287 24,000 58
TaqMan TaqMan HPLC TaqMan MGB TaqMan 3' Tm Minor Groove Binder MGB TaqMan TAMRA Tm TaqMan MGB Probe Small size 6,000pmol 4316034 62,000 TaqMan MGB Probe Medium size 20,000pmol 4316033 129,300 TaqMan MGB Probe Large size 50,000pmol 4316032 165,700 14. TaqMan TAMRA First Choice Second Choice DNA/RNA FAM VIC Minor Allele Major Allele SNP FAM VIC * FAM VIC TaqMan TAMRA Probe Small size 6,000pmol 450025 52,000 TaqMan TAMRA Probe Medium size 20,000pmol 450024 98,000 TaqMan TAMRA Probe Large size 50,000pmol 450003 136,400 TaqMan MGB MGB TaqMan MGB Small size 4324034 65,000 6,000pmol 1 27,000pmol 2 TaqMan MGB Medium size 4324035 135,400 20,000pmol 1 90,000pmol 2 TaqMan MGB Large size 4324036 176,800 50,000pmol 1 225,000pmol 2 TaqMan MGB SNP MGB 2 TaqMan MGB SNPSmall size 4323297 120,000 6,000pmol 2 27,000pmol 2 TaqMan MGB SNPMedium size 4323298 252,500 20,000pmol 2 90,000pmol 2 TaqMan MGB SNPLarge size 4323300 323,200 50,000pmol 2 225,000pmol 2 TaqMan TAMRA TAMRA TaqMan Probe 4323969 56,600 6,000pmol 1 27,000pmol 2 TaqMan TaqMan TaqMan Universal Master Mix II TaqMan Gene Expression Master Mix TaqMan Universal PCR Master Mix TaqMan Fast Universal PCR Master Mix TaqMan Fast Advanced Master Mix SYBR Green Power SYBR Green PCR Master Mix Fast SYBR Green Master Mix SYBR Green PCR Master Mix SYBR Select Master Mix TaqMan SYBR Green * 250nM 900nM 50nM 900nM 50nM * 2 59
TaqMan Assays sirna ID E www.appliedbiosystems.jp TAQMAN ASSAYS TaqMan Gene Expression Assays, Inventoried 24 TaqMan Gene Expression Assays, Made to Order 24 Custom TaqMan Gene Expression Assays 24 Custom Plus TaqMan RNA Assays 24 TaqMan Array Plate 25 TaqMan Custom Arrays 28 Silencer Select sirna 33 TaqMan sirna Assays 35 TaqMan Micro RNA Assays 40 Custom TaqMan Small RNA Arrays 40 TaqMan Pri-miRNA Assays 42 TaqMan Non-coding RNA Assays 42 mirvana mirna mimics 43 mirvana mirna Inhibitors 43 Pre-miR mirna Precursor Molecule 43 Anti-miR mirna Inhibitor 43 TaqMan SNP Genotyping Assays 45 Custom TaqMan SNP Genotyping Assays 45 TaqMan Drug Metabolism Genotyping Assays 45 TaqMan Mutation Detection Assays 48 TaqMan Copy Number Assays 49 Custom TaqMan Copy Number Assays 49 Applied Biosystems ID E Applied Biosystems www.appliedbiosystems.jp E TaqMan MGB 59 TaqMan 59 E JPOrderAdminCS@appliedbiosystems.com 03-6832-6980 60
TaqMan Custom Plating Service TaqMan Custom Plating ServiceTaqMan Assay 96 384 TaqMan Gene Expression Assays TaqMan MicroRNA Assays SNP Genotyping Assay Copy Number Assay Non coding RNA Assay srrna TaqMan Gene Expression Assays, Inventoried TaqMan Gene Expression Assays, Made-to-order TaqMan MicroRNA Assays, Inventoried TaqMan MicroRNA Assays, Made-to-order TaqMan Non-coding RNA Assays, Inventoried TaqMan Non-coding RNA Assays, Made-to-order TaqMan SNP Genotyping Assays DME TaqMan Copy Number Assays 96 Standard 96 Fast 384 10 µl 384 20 µl 96 20 61
INDEX -1-4304886 22 4326314E 30 4346800 28 10296010 10 4304965 22 4326315E 30 4346802 28 10296028 10 4305719 20 4326316E 30 4346906 57 11754050 16 4306736 22 4326317E 30 4346907 57 11754250 16 4306737 57 4326318E 30 4351368 24 11755050 16 4307281 17 4326319E 30 4351370 24 11755250 16 4309155 22 4326320E 30 4351372 24 11755550 16 4309169 15 4326321E 30 4351374 45 12183016 10 4309224 58 4326322E 30 4351376 45 12183018A 10 4309849 57 4326323E 30 4351379 45 12183020 10 4310251 22 4326614 20 4351380 45 12183025 10 4310299 15 4326659 57 4351382 45 12183026 10 4311235 17 4326708 20 4351384 45 12183555 10 4311971 57 4331182 24,30 4351891 18 12185010 10 4312063 58 4331348 24 4352042 18 1377501 36 4312639 58 4331349 45 4352338E 31 1377505 36 4312660 17 4332072 45 4352339E 31 13778-030 36 4312704 22 4332073 45 4352340E 31 13778-075 36 4313663 57 4332075 45 4352341E 31 13778-100 36 4313803 15 4332076 45 4352405 21 13778-150 36 4316032 59 4332077 45 4352930E 30,31 13778-500 36 4316033 59 4332078 24 4352931E 31 15596018 10 4316034 59 4332079 24 4352932E 31 15596018SP 10 4316567 57 4333183 57 4352933E 31 15596026 10 4318157 20 4334973 22 4352934E 30-4- 4319413E 30,31 4342247 28 4352935E 30 402823 21 4323032 57 4342249 28 4358293 58 402877 15 4323297 59 4342253 28 4358297 58 402929 17 4323298 59 4342259 28 4360954 57 402930 21 4323300 59 4342261 28 4362691 45 403012 57 4323969 59 4342265 28 4364103 18 403028 15 4324018 20 4343370 57 4364338 20 403081 58 4324020 20 4343814 57 4364340 20 4304134 17 4324034 59 4344463 22 4364341 20 4304437 20 4324035 59 4345287 58 4364343 20 4304441 21 4324036 59 4346798 28 4366072 18 4304449 20 4326270 57 4346799 28 4366073 18 63
INDEX 4366596 39 4375816 58 4390771 34 4392938 15 4366597 39 4375928 58 4390815 34 4393463 15 4366932 57 4378700 28 4390816 34 4393464 15 4367218 22 4378701 28 4390817 34 4396840 27,29 4367563 28,29 4378702 28 4390818 34 4397914 34 4367659 22 4378703 28 4390821 34 4397915 34 4367660 22 4378704 28 4390822 34 4397916 34 4367784 28 4378705 28 4390824 34 4397917 34 4367785 28 4378706 28 4390825 34 4397918 34 4367786 28 4378707 28 4390826 34 4397919 34 4367846 18 4378708 28 4390828 34 4397920 34 4368577 22 4378709 28 4390829 34 4397921 34 4368702 22 4378710 28 4390830 34 4397922 34 4368706 22 4378713 28 4390831 34 4397923 34 4368708 22 4378714 28 4390843 34 4397924 34 4368813 17 4379590 58 4390844 34 4397926 34 4368814 17 4379961 28 4390846 34 4397927 34 4369016 20 4379983 58 4390847 34 4398965 28,41 4369510 20 4381656 46 4390849 34 4398966 28,41 4369514 20 4381657 46 4390850 34 4398967 28,41 4369542 20 4381850 58 4391003 15 4398968 28,41 4370048 20 4384267 21 4391112 15 4398987 40 4370074 20 4385344 28 4391128 21 4398988 40 4370573 28 4385363 28 4391178 15 4398989 40 4371130 46 4385371 22 4391524 25 4399002 13 4371131 46 4385372 22 4391525 25 4399003 13 4371134 20 4385610 22 4391526 25 4399201 39 4371135 20 4385612 22 4391527 25 4399203 39 4371353 46 4385614 22 4391528 25 4399233 39 4371355 46 4385616 22 4391529 25 4399237 39 4371357 46 4385617 22 4391848 13 4399966 39 4374966 17 4385618 22 4392339 17 4399968 39 4374967 17 4386995 13 4392420 34 4399970 39 4375282 58 4387299 13 4392421 34 4399972 39 4375284 58 4387406 17 4392422 34 4400088 53 4375323 58 4387951 17 4392653 15 4400238 28,41 4375575 17 4389986 15 4392656 15 4400239 28,41 64
INDEX 4400291 49 4405443 54 4414087 26 4414124 26 4400292 49 4405448 54 4414088 26 4414125 26 4400293 49 4405460 53 4414089 26 4414126 26 4400294 49 4405465 53 4414090 26 4414127 26 4400295 49 4405471 53 4414091 26 4414128 26 4400296 49 4405501 53 4414092 26 4414129 26 4400925 39 4409535 51 4414093 26 4414130 26 4400926 39 4410126 51 4414094 26 4414131 26 4400927 39 4413255 25 4414095 26 4414132 26 4400928 39 4413256 25 4414096 26 4414133 26 4401009 39 4413257 25 4414097 26 4414134 26 4401010 39 4413258 25 4414098 26 4414135 26 4401011 39 4413259 25 4414099 26 4414136 26 4401012 39 4413260 25 4414100 26 4414137 26 4401090 39 4413261 25 4414101 26 4414138 26 4401091 39 4413262 25 4414102 26 4414139 26 4401857 46 4413263 25 4414103 26 4414140 26 4401890 46 4413264 25 4414104 26 4414141 26 4401892 46 4413265 25 4414105 26 4414142 26 4402953 13 4413266 25 4414106 26 4414143 26 4402954 13 4414070 26 4414107 26 4414144 26 4402955 13 4414071 26 4414108 26 4414145 26 4402956 13 4414072 26 4414109 26 4414146 26 4402957 13 4414073 26 4414110 26 4414147 26 4402959 13 4414074 26 4414111 26 4414148 27 4402960 13 4414075 26 4414112 26 4414149 27 4403081 47 4414076 26 4414113 26 4414150 27 4403083 47 4414077 26 4414114 26 4414151 27 4403087 47 4414078 26 4414115 26 4414152 27 4403311 46 4414079 26 4414116 26 4414153 27 4403313 47 4414080 26 4414117 26 4414154 27 4403315 49 4414081 26 4414118 26 4414155 27 4403316 49 4414082 26 4414119 26 4414156 27 4403326 49 4414083 26 4414120 26 4414157 27 4403328 49 4414084 26 4414121 26 4414158 27 4404020 34 4414085 26 4414122 26 4414159 27 4404024 34 4414086 26 4414123 26 4414160 27 65
INDEX 4414161 27 4414198 27 4418745 26 4418782 26 4414162 27 4414199 27 4418746 26 4418783 26 4414163 27 4414200 27 4418747 26 4418784 26 4414164 27 4415440 51 4418748 26 4418785 26 4414165 27 4415450 51 4418749 26 4418786 26 4414166 27 4415452 51 4418750 26 4418787 26 4414167 27 4415461 27 4418751 26 4418788 26 4414168 27 4418715 26 4418752 26 4418789 26 4414169 27 4418716 26 4418753 26 4418790 26 4414170 27 4418717 26 4418754 26 4418791 26 4414171 27 4418718 26 4418755 26 4418792 26 4414172 27 4418719 26 4418756 26 4418793 27 4414173 27 4418720 26 4418757 26 4418794 27 4414174 27 4418721 26 4418758 26 4418795 27 4414175 27 4418722 26 4418759 26 4418796 27 4414176 27 4418723 26 4418760 26 4418797 27 4414177 27 4418724 26 4418761 26 4418798 27 4414178 27 4418725 26 4418762 26 4418799 27 4414179 27 4418726 26 4418763 26 4418800 27 4414180 27 4418727 26 4418764 26 4418801 27 4414181 27 4418728 26 4418765 26 4418802 27 4414182 27 4418729 26 4418766 26 4418803 27 4414183 27 4418730 26 4418767 26 4418804 27 4414184 27 4418731 26 4418768 26 4418805 27 4414185 27 4418732 26 4418769 26 4418806 27 4414186 27 4418733 26 4418770 26 4418807 27 4414187 27 4418734 26 4418771 26 4418808 27 4414188 27 4418735 26 4418772 26 4418809 27 4414189 27 4418736 26 4418773 26 4418810 27 4414190 27 4418737 26 4418774 26 4418811 27 4414191 27 4418738 26 4418775 26 4418812 27 4414192 27 4418739 26 4418776 26 4418813 27 4414193 27 4418740 26 4418777 26 4418814 27 4414194 27 4418741 26 4418778 26 4418815 27 4414195 27 4418742 26 4418779 26 4418816 27 4414196 27 4418743 26 4418780 26 4418817 27 4414197 27 4418744 26 4418781 26 4418818 27 66
INDEX 4418819 27 4426961 42 4444432 21 4453745 53 4418820 27 4426962 42 4444434 21 4457287 35 4418821 27 4426963 42 4444436 21 4457288 35 4418822 27 4427012 42 4444556 18 4457289 35 4418823 27 4427013 42 4444557 18 4457291 35 4418824 27 4427014 42 4444558 18 4457292 35 4418825 27 4427037 34 4444745 39 4457296 35 4418826 27 4427038 34 4444746 39 4457297 35 4418827 27 4427975 40 4444747 39 4457298 35 4418828 27 4440038 19 4444748 39 4457301 35 4418829 27 4440039 19 4444749 39 4457302 35 4418830 27 4440040 19 4444750 39 4457303 35 4418831 27 4440041 19 4444752 39 4457304 35 4418832 27 4440042 19 4444766 39 4457307 35 4418833 27 4440043 19 4444899 41 4457308 35 4418834 27 4440044 19 4444909 41 4457309 35 4418835 27 4440045 19 4444910 28,41 4457310 35 4418836 27 4440046 19 4444913 28,41 4458235 14 4418837 27 4440047 19 4444963 18 4458236 14 4418838 27 4440048 19 4444964 18 4458237 14 4418839 27 4440049 19 4444965 18 4458366 49 4418840 27 4440878 35 4445552 14 4458367 49 4418841 27 4440879 35 4445553 14 4458368 49 4418842 27 4440880 35 4445554 14 4458369 49 4418843 27 4440886 40 4445555 14 4459405 35 4418844 27 4440887 40 4448484 24 4459407 35 4418845 27 4440888 40 4448485 24 4459408 35 4418846 27 4441114 24 4448486 24 4461146 55 4425557 51 4441117 24 4448489 24 4462263 55 4425559 51 4441118 24 4448490 24 4464058 43 4425562 51 4442487 49 4448491 24 4464059 43 4425618 51 4442488 49 4448536 54 4464060 43 4426697 27,29 4442520 49 4448549 53 4464061 43 4426698 27,29 4444281 39 4448571 53 4464062 43 4426699 27,29 4444292 39 4448591 53 4464063 43 4426700 27,29 4444303 39 4448592 53 4464064 43 4426701 27,29 4444308 39 4448616 53 4464065 43 67
INDEX 4464066 43 4464067 43 4464068 43 4464069 43 4464070 43 4464071 43 4464074 43 4464075 43 4464076 43 4464077 43 4464078 43 4464079 43 4464080 43 4464081 43 4464084 43 4464085 43 4464086 43 4464087 43 4464088 43 4464089 43 4464092 43 4464093 43 4465804 48 4465805 48 4465807 48 4466037 55 4466038 55 4467538 48 4469576 9 4469607 9 4470184 9 4470185 9 4472103 9 4472903 23 4472908 23 4472913 23 4472918 23 AM1911 11 AM1912 11 AM1914 11 AM1920 11 AM1931 11 AM1939 11 AM7000 11 AM7001 11 AM7020 11 AM7021 11 AM7022 11 AM7023 11 AM7024 11 AM7030 11 AM9860 11 AM9861 11 AM9912 11 AM9930 20 AM9932 20 AM9937 20 AM9938 20 AM9939 20 -N- N8010560 57 N8010933 57 N8080096 17 N8080119 17 N8080127 17 N8080128 17 N8080228 21 N8080234 17 N8080236 15 N8080260 17 N8080270 17 4472919 23 4472920 23 4473087 38 4473088 38 450003 59 450024 59 450025 59 -A- AM10026 11 AM10027 11 AM10050 11 AM1540 43 AM1556 38 AM1560 38 AM1561 38 AM17000 43 AM17001 43 AM17003 43 AM17010 43 AM17011 43 AM17012 43 AM17100 43 AM17101 43 AM17103 43 AM17110 43 AM17111 43 AM17120 43 AM17121 43 AM17150 43 AM1728 13 AM1729 13 AM1812 11 AM1830 11 AM1836 11 AM1837 11 AM1840 11 AM1907 11 68
INDEX Customer-Defined mirvana mirna Mimic 43 Cy3 Anti-miR Negative Control 43 Cy3 Pre-miR Negative Control 43 -D- DigitalSuite Software 9 -F- FAM Anti-miR Negative Control 43 FAM Pre-miR Negative Control 43 Fast SYBR Green Cells-to-CT Kit 13 Fast SYBR Green Master Mix 22 -G- GeneAmp dntp Blend 17 -H- High Capacity cdna Reverse Transcription Kit 17 High Capacity RNA-to-cDNA Kit 17 -I- Invivofectamine 2.0 Reagent 36 Invivofectamine 2.0 Reagent Starter Kit 36 -L- LAMP for Applied Biosystems 7300/7500/ViiA 7 System 58 LAMP for GeneAmp 5700 and ABI PRISM 7000 System 58 Lipofectamine RNAiMAX 36 -M- MagMAX Total Nucleic Acid Isolation Kit 11 MagMAX Viral RNA Isolation Kit 11 MagMAX -96 Blood RNA Isolation Kit 11 MagMAX -96 Total RNA Isolation Kit 11 MagMAX -96 Viral RNA Isolation Kit 11 Magnetic Stand-96 11 Megaplex PreAmp Primers, Human Pool 39 Megaplex PreAmp Primers, Human Pool Set 39 Megaplex PreAmp Primers, Rodent Pool 39 Megaplex PreAmp Primers, Rodent Pool Set 39 Megaplex Primer Pools, Human Pools 39 Megaplex Primer Pools, Human Pools Set 39 Megaplex Primer Pools, Rodent Pools 39 Megaplex Primer Pools, Rodent Pools Set 39-0- 0.1 mm EDTA 11-2- 20% Glycerol Solution 17-9- 96 well Magnetic-Ring Stand 11 -A- Ambion In Vivo Custom Designed 35 Ambion In Vivo Custom sirna 35 Ambion In Vivo Factor VII Positive Control sirna, HPLC In Vivo Ready 35 Ambion In Vivo Factor VII sirna, HPLC In Vivo Ready 35 Ambion In Vivo GAPDH Positive Control sirna 35 Ambion In Vivo Negative Control #1 sirna 35 Ambion In Vivo Pre-designed sirna 35 Ambion Single Cell Lysis Kit 14 Ambion Single Cell-to-CT Kit 14 AmpErase Uracil N-glycosylase UNG 17 Anti-miR mirna Inhibitor 43 Anti-miR Negative Control #1 43 -C- Cells-to-CpG Bisulfite Conversion & Quantitation Control Kit 14 Cells-to-CpG Bisulfite Conversion Kit 14 Cells-to-CpG Methylated and Unmethylated gdna Control Kit 14 Cells-to-CT Stop Solution 13 Custom Plus TaqMan Copy Number Assays 49 Custom Plus TaqMan RNA Assay 24 Custom Select sirna 34 Custom TaqMan Copy Number Assays 49 Custom TaqMan Gene Expression Assays 24 Custom TaqMan Small RNA Assays 40 Custom TaqMan SNP Genotyping Assays 45 Customer Defined Anti-miR mirna Inhibitor 43 Customer Defined Pre-miR mirna Precursor 43 Customer-Defined mirvana mirna Inhibitor 43 69
INDEX Megaplex RT Primers, Human Pool 39 Megaplex RT Primers, Human Pool Set 39 Megaplex RT Primers, Rodent Pool 39 Megaplex RT Primers, Rodent Pool Set 39 MeltDoctor HRM Master Mix 51 MeltDoctor HRM 51 MeltDoctor HRM 51 MeltDoctor HRM 51 MeltDoctor HRM 51 MicroAmp 48-Well Base Adaptor 58 MicroAmp 96-Well Support Base 58 MicroAmp 96-Well Tray for VeriFlex Blocks 58 MicroAmp 96-Well Tray/Retainer Set 58 MicroAmp 96-Well Tray/Retainer Set for Veriti System 58 MicroAmp Adhesive Film Applicator 57 MicroAmp Fast 48-Well Trays 58 MicroAmp Fast 8-Tube Strip 0.1mL 58 MicroAmp Fast 96-Well Reaction Plate 0.1mL 57 MicroAmp Fast Optical 48-Well Reaction Plate 58 MicroAmp Fast Optical 96-Well Reaction Plate with Barcode 0.1mL 57 MicroAmp Fast Reaction Tube with Cap 0.1mL 58 MicroAmp Optical 384-Well Reaction Plate 57 MicroAmp Optical 384-Well Reaction Plate with Barcode 57 MicroAmp Optical 48-Well Adhesive Film 58 MicroAmp Optical 8-Cap Strip Flat 57 MicroAmp Optical 8-Tube Strip 0.2mL 57 MicroAmp Optical 96-Well Reaction Plate 57 MicroAmp Optical 96-Well Reaction Plate with Barcode 57 MicroAmp Optical 96-Well Reaction Plate with Barcode and Optical Caps 57 MicroAmp Optical Adhesive Film 57 MicroAmp Optical Adhesive Film Kit 57 MicroAmp Optical Film Compression Pad 58 MicroAmp Optical Tube without Cap 0.2mL 57 MicroAmp Splash Free 96-Well Base 58 mirvana mirna inhibitor 43 mirvana mirna inhibitor 43 mirvana mirna Inhibitor let-7 Positive Control 43 mirvana mirna Inhibitor Negative Control #1 43 mirvana mirna Isolation Kit 38 mirvana mirna mimic 43 mirvana mirna Mimic mir-1 Positive Control 43 mirvana mirna Mimic Negative Control #1 43 mirvana mirna mimics 43 mirvana PARIS Kit 38 MultiScribe Reverse Transcriptase 17 -N- Nuclease-free Water 20 -O- Oligo d(t) 16 Primers 17 -P- Power SYBR Green Cells-to-CT Kit 13 Power SYBR Green PCR Master Mix 22 Power SYBR Green RNA-to-CT 1-Step Kit 15 Pre-miR hsa-mir-1 mirna Precursor 43 Pre-miR mirna Precursor Starter Kit 43 Pre-miR mirna Precursors Molecule 43 Pre-miR Negative Control 43 Protein Expression Lysate Control Kit 54 Protein Expression Sample Prep Kit 54 Protein Quant Sample Lysis Kit 54 Protein Thermal Shift Dye Kit 55 Protein Thermal Shift Starter Kit 55 Protein Thermal Shift 1.0 55 Protein Thermal Shift 55 PureLink DNase Set 10 PureLink Homogenizer 10 PureLink RNA Micro Kit 10 PureLink RNA Mini Kit 10 -Q- QuantStudio 12K Flex OpenArray Accessories Kit 9 QuantStudio 12K Flex OpenArray Digital PCR Starter Kit 9 70
INDEX SuperScript VILO cdna Synthesis Kit 16 SuperScript VILO Master Mix 16 SYBR Green Cells-to-CT Control Kit 13 SYBR Green PCR Core Reagents 22 SYBR Green PCR Master Mix 22 SYBR Select Master Mix 23 -T- TaqMan Array Gene Signature 96-Well Plate 26,27 TaqMan Array Human Endogenous Control Plate 29 TaqMan Array Human Endogenous Controls 29 TaqMan Array Human MicroRNA Cards 28,41 TaqMan Array Human MicroRNA Cards Set 28,41 TaqMan Array Mouse Endogenous Controls 29 TaqMan Array Plates 25 TaqMan Array Rat Endogenous Controls 29 TaqMan Array Rodent MicroRNA Cards 28,41 TaqMan Array Rodent MicroRNA Cards Set 28,41 TaqMan Custom Arrays 28 TaqMan Cells-to-CT Control Kit 13 TaqMan Control Genomic DNA Human 17 TaqMan Control Total RNA Human 17 TaqMan Copy Number Assays 49 TaqMan Copy Number Reference Assay 49 TaqMan Drug Metabolism Genotyping Assay 45 TaqMan EGFR Exon 19 Deletions Assay 48 TaqMan Endogenous Control 30,31 TaqMan EZ RT-PCR Core Reagents 15 TaqMan Fast Advanced Master Mix 18 TaqMan Fast Cells-to-CT Kit 13 TaqMan Fast Universal PCR Master Mix 18 TaqMan Fast Virus 1-Step Master Mix 21 TaqMan Gene Expression Assays, Inventoried 24,30 TaqMan Gene Expression Assays, Made-to-Order 24 TaqMan Gene Expression Cells-to-CT Kit 13 TaqMan Gene Expression Master Mix 20 TaqMan Gene Signature Arrays 28 TaqMan Genotyping Master Mix 46 QuantStudio Digital PCR Kit 9 QuantStudio 12K FlexPCR OpenArray 9 -R- Random Hexamers 17 RNA later -ICE 11 RNA later Solution 11 RNAqueous Kit 11 RNAqueous Midi Kit 11 RNAqueous -4PCR Kit 11 RNAqueous -96 Automated Kit 11 RNAqueous -96 Kit 11 RNAqueous -Micro Kit 11 RNase Inhibitor 17 -S- Silencer Select GAPDH sirna (Hs, Mm, Rn) 34 Silencer Select Human Druggable Genome sirna Library V4 34 Silencer Select Human Druggable Genome V4 sirna Ext Set 34 Silencer Select Human Extended Druggable Genome sirna Library V4 34 Silencer Select Human Genome sirna Library V4 34 Silencer Select Human Genome sirna V4 Extension Set 34 Silencer Select Human GPCR sirna Library V4 34 Silencer Select Human Ion Channel sirna Library V4 34 Silencer Select Human Kinase sirna Library V4 34 Silencer Select Human Nuclear Hormone Receptor sirna Library V4 34 Silencer Select Human Phosphatase sirna Library V4 34 Silencer Select Human Protease sirna Library V4 34 Silencer Select Negative Control sirna 34 Silencer Select Pre-designed sirna 34 Silencer Select Validated sirna 34 Single Place Magnetic Stand 11 71
INDEX TaqMan Gold with Buffer A Pack 21 TaqMan GTXpress Master Mix 46 TaqMan Human Endogenous Control Array 29 TaqMan MGB Probe 59 TaqMan MGB SNP 59 TaqMan MGB 59 TaqMan MicroRNA Assays, Inventoried 40 TaqMan MicroRNA Assays, Made-to-Order 40 TaqMan MicroRNA Cells-to-CT Kit 13 TaqMan MicroRNA RT Kit 39 TaqMan mirna ABC Purification Kit-Human Panel 38 TaqMan Mutation Detection Assays 48 TaqMan Mutation Detection IPC Reagent Kit 48 TaqMan Mutation Detection Reference Assays 48 TaqMan Non-coding RNA Assays 42 TaqMan One-Step RT-PCR Master Mix Reagents Kit 15 TaqMan PCR Core Reagents Kit 21 TaqMan Pre-Designed SNP Genotyping Assays 45 TaqMan PreAmp Cells-to-CT Kit 13 TaqMan PreAmp Master Mix 21 TaqMan PreAmp Master Mix Kit 21 TaqMan Pri-miRNA Assays 42 TaqMan Probe 59 TaqMan Protein Assay Kit 53 TaqMan Protein Assays Buffer Kit 53 TaqMan Protein Assays Core Reagents Base Kit 53 TaqMan Protein Assays Fast MasterMix 53 TaqMan Protein Assays Oligo Probe Kit 53 TaqMan Protein Assays Open Kit 53 TaqMan Protein Expression Core Reagents Kit with MasterMix 53 TaqMan Reagents Starter Kit 21 TaqMan Reverse Transcription Reagents 17 TaqMan RNA-to-CT 1-Step Kit 15 TaqMan Sample-to-SNP Kit 47 TaqMan sirna Assays, Made-to-Order 35 TaqMan TAMRA Probe 59 TaqMan Universal Master Mix II, no UNG 19 TaqMan Universal Master Mix II, with UNG 19 TaqMan Universal PCR Master Mix 20 TaqMan Universal PCR Master Mix, No AmpErase UNG 20 TE Buffer ph7.0 11 THE RNA Storage Solution 11 TRIzol LS Reagent 10 TRIzol Plus RNA Purification System 10 TRIzol Reagent 10 TURBO DNA-free Kit 11 72
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