バイオロジカルスキャホールド

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特集再生医療幹細胞操作と工学技術からのアプローチ Tetsuji YAMAOKA 1. はじめに 1993 MIT Langer 1) Tissue Engineering 2010 11 extra cellular matrix; ECM biological scaffolds 2.

1 特集再生医療幹細胞操作と工学技術からのアプローチ Tetsuji YAMAOKA 1. はじめに 1993 MIT Langer 1) Tissue Engineering extra cellular matrix; ECM biological scaffolds 2. バイオロジカルスキャホールド 2001 ECM bioderived scaffold 著者連絡先 biomimetic scaffold ECM acellular scaffold biologic scaffold 3. 生体由来スキャホールド (bioderived scaffold) 1986 guided tissue regeneration GTR 2) 4) 5) 1980 Yannas 6) 7), 8) ECM Langer ECM 231

2 5μm 5μm 5μm 図 1 OLA-peptide A B C ECM 9) 4. 生体模倣スキャホールド (biomimetic scaffolds) Arg-Gly-Asp RGD 1989 Ile-Lys-Val-Ala-Val IKVAV 10) 12) Silva 13) PLA IKVAV PLA OLA peptide OLA-peptide 図 1 Fmoc IKVAV 7 20 acola OLA-IKVAV 1 20 w/v 1,1,1,3,3,3-hexafluoro-2- propanol; HFIP A B C g 10 mm 14 mm 図 2 A,B 2 mm 10 mm 図 2C, D 4 OLA-IKVAV PLA OLA-IKVAV 14),15) ECM 2006 Discher MSCs 232

LifeCell 20) SDS X-100 2000 980 MPa 図 4 Ca 2 PO4 2 3 a

3 図 3 21) (a) 図 2 in vivo A PLA SEM B C D (b) 16) 5. 脱細胞スキャホールド (acellular scaffold) 17) ),19) AlloDerm LifeCell 20) SDS X MPa 図 4 Ca 2 PO4 2 3 a vonkossa b CT 図 3 21) 1 DNase DNA 80 21) 図 4 Ca 2 PO4 2 DNase DNA Simionescu 233

図 5 a PEG b X-100 c SDS Reprinted by permission from Macmillan Publisher Ltd: Nature Medicine 14: 214, 2008. 23) 22) 図 5 Ott a PEG b X-100 c SDS 23) SDS 6.

4 図 5 a PEG b X-100 c SDS Reprinted by permission from Macmillan Publisher Ltd: Nature Medicine 14: 214, ) 22) 図 5 Ott a PEG b X-100 c SDS 23) SDS 6. おわりに in vivo 文献 1) Langer R, Vacanti JP: Tissue engineering. Science 260: 920-6, ) Aukhil I, Pettersson E, Suggs C: Guided tissue regeneration. An experimental procedure in beagle dogs. J Periodontol 57: , ) Gottlow J, Nyman S, Lindhe J, et al: New attachment formation in the human periodontium by guided tissue regeneration. Case reports. J Clin Periodontol 13: , ) Dahlin C, Linde A, Gottlow J, et al: Healing of bone defects by guided tissue regeneration. Plast Reconstr Surg 81: 672-6, ) 28: 24-35, ) Yannas IV, Burke JF: Design of an artificial skin. I. Basic design principles. J Biomed Mater Res 14: 65-81, ) Archibald SJ, Krarup C, Shefner J, et al: A collagen-based ner ve guide conduit for peripheral ner ve repair: an electrophysiological study of nerve regeneration in rodents and nonhuman primates. J Comp Neurol 306: , ) Nakamura T, Inada Y, Fukuda S, et al: Experimental study 234

5 on the regeneration of peripheral nerve gaps through a polyglycolic acid-collagen (PGA-collagen) tube. Brain Res 1027: 18-29, ) Liu W, Merrett K, Griffith M, et al: Recombinant human collagen for tissue engineered cor neal substitutes. Biomaterials 29: , ) Tashiro K, Sephel GC, Weeks B, et al: A synthetic peptide containing the IKVAV sequence from the A chain of laminin mediates cell attachment, migration, and neurite outgrowth. J Biol Chem 264: , ) Schense JC, Bloch J, Aebischer P, et al: Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension. Nat Biotechnol 18: 415-9, ) Gunn JW, Turner SD, Mann BK: Adhesive and mechanical proper ties of hydrogels influence neurite extension. J Biomed Mater Res A 72: 91-7, ) S i l v a G A, C z e i s l e r C, N i e c e K L, e t a l : S e l e c t i v e differentiation of neural progenitor cells by high-epitope density nanofibers. Science 303: , ) ) Kakinoki S, Uchida S, Ehashi T, et al: Surface modification of poly (L-lactic acid) nanofiber with oligo (D-lactic acid) bioactive-peptide conjugates for peripheral ner ve regeneration. Polymers 3: , ) Engler AJ, Sen S, Sweeney HL, et al: Matrix elasticity directs stem cell lineage specification. Cell 126: , ) Badylak S, Liang A, Record R, et al: Endothelial cell adherence to small intestinal submucosa: an acellular bioscaffold. Biomaterials 20: , ) Schmidt CE, Baier JM: Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering. Biomaterials 21: , ) Steinhoff G, Stock U, Karim N, et al: Tissue engineering of pulmonar y hear t valves on allogenic acellular matrix conduits: in vivo restoration of valve tissue. Circulation 102: III50-5, ) Crapo PM, Gilbert TW, Badylak SF: An overview of tissue and whole organ decellularization processes. Biomaterials 32: , ) Fujisato T, Minatoya K, Yamazaki S, et al: Cardiovascular Regeneration Therapies Using Tissue Engineering Approaches, H. Mori and H. Matsuda (Eds), Springer, Tokyo, 2005, 83 22) Tedder ME, Liao J, Weed B, et al: Stabilized collagen scaffolds for heart valve tissue engineering. Tissue Eng Part A 15: , ) Ott HC, Matthiesen TS, Goh SK, et al: Per fusiondecellularized matrix: using nature s platform to engineer a bioartificial heart. Nat Med 14: ,

人工神経

Tatsuo NAKAMURA 1. はじめに BAHA 2013 1 1 brain computer interface 1) motor function 著者連絡先 606-850753 E-mail. nakamura@frontier.kyoto-u.ac.jp 2) 4) 2. 人工神経開発のベースとなるin situ Tissue Engineeringという概念 Tissue Engineering