Phenomenex年度末応援セール2018

Transcription

1 2~5% FF LC GC peep! Happy New Year! F F

2 21832 CAMP UHPLC/HPLC EV C18 C18 Si XB- C18 C8 ACN H 2 ACN ACN ACN F ACN ACN ACN ACN F F ACN ACN ACN ACN H 2 ACN ACN ACN ACN H 2 H 2 H 2 Polar Polar F F H 2 H H H 2 H H 2 H 2 H 2 H H 2 Si H 2 H Si Si H mm Polar C18 F5 Bi phenyl Ph enyl- Hexyl HILIC UHPLC/HPLC UHPLC/HPLC MEGA Polar Polar + + Bio Core-Shell Technology Aeris WIDEPRE 3. μm Aeris PEPTIDE 7 Luna mega 4. mm Luna mm LC UHPLC HPLC ULTRA UHPLC Column Protection HPLC Column Protection mm Phenomenex l WEB:

3 3 % FF 5 % FF LC/SFC (GFC/SEC) Chiral LC Columns Ultra-High Efficiency GFC/SEC mm μm 7.8 mm (GPC/SEC) rganic GPC/SEC Ion Exclusion LC 17 1 mm mm GC GC Columns Polymeric SPE (SPE) Phospholipid Removal Solutions 1819 Solid Phase Extraction (SPE) 223 β-glucuronidase Removal Products β- simplified liquid extraction (SLE) 3

4 LC Kinetex HPLC UHPLC Kinetex Phenomenex Kinetex Kinetex 4 Phenomenex l WEB:

5 Polar C18 EV C18 C18 XB- C18 C8 C18 1% ph * 2. μm USP L1 C18 1% ph μm, 2. μm, 1.7 μm USP L1 C18 (DS) ph * 5 μm, 2. μm, 1.7 μm, 1.3 μm USP L1 C18 ph * 5 μm, 3.5 μm, 2. μm, 1.7 μm USP L1 C18 C8 USP L7 ph * 5 μm, 2. μm, 1.7 μm USP L7 F5 Bi phenyl Ph enyl- Hexyl HILIC (PFP) 1% ph μm, 2. μm, 1.7 μm USP L43 ph * 5 μm, 2. μm, 1.7 μm USP L11 * ph ph 1.51 (C-Phenyl) ph * 5 μm, 2. μm, 1.7 μm USP L11 HILIC ph μm, 2. μm, 1.7 μm USP L3 4. mm 2. μm Minibore (mm) SecurityGuard ULTRA Phases 3 x x x x x 2.1 3/pk 57, 1, 7, 74, 3, 72, 77, 87,5 92,5 45, EV C18 A-4725-AN B-4725-AN D-4725-AN F-4725-AN AJ ,2 59,2 5, 7,2 71,2 3, 9, 74, 82, 84, 89, 45, Polar C18 A-4759-AN B-4759-AN D-4759-AN F-4759-AN AJ-9532 F5 A-4723-AN B-4723-AN D-4723-AN F-4723-AN AJ-9322 Biphenyl A-422-AN B-422-AN D-422-AN F-422-AN AJ-929 XB-C18 A-449-AN B-449-AN C-449-AN D-449-AN F-449-AN AJ-8782 C18 A-442-AN B-442-AN C-442-AN D-442-AN F-442-AN AJ-8782 C8 A-4497-AN B-4497-AN C-4497-AN D-4497-AN F-4497-AN AJ-8784 HILIC A-441-AN B-441-AN C-441-AN D-441-AN F-441-AN AJ-878 Phenyl-Hexyl A-4495-AN B-4495-AN C-4495-AN D-4495-AN F-4495-AN AJ mm 2. μm MidBore (mm) SecurityGuard ULTRA Phases 3 x 3. 5 x x 3. 1 x x 3. 3/pk 1, 7, 74, 3, 77, 87,5 92,5 45, EV C18 B-4725-Y D-4725-Y F-4725-Y AJ ,2 59,2 5, 7,2 71,2 3, 9, 74, 82, 84, 89, 45, Polar C18 B-4759-Y D-4759-Y F-4759-Y AJ-9531 F5 B-4723-Y D-4723-Y F-4723-Y AJ-9321 Biphenyl B-422-Y D-422-Y F-422-Y AJ-928 XB-C18 A-449-Y B-449-Y C-449-Y D-449-Y F-449-Y AJ-8775 C18 A-442-Y B-442-Y C-442-Y D-442-Y F-442-Y AJ-8775 C8 A-4497-Y B-4497-Y C-4497-Y D-4497-Y F-4497-Y AJ-8777 HILIC A-441-Y F-441-Y AJ-8779 Phenyl-Hexyl B-4495-Y D-4495-Y F-4495-Y AJ mm 2. μm Analytical (mm) SecurityGuard ULTRA Phases 3 x 4. 5 x x 4. 1 x x 4. 3/pk 1, 7, 74, 3, 77, 87,5 92,5 45, EV C18 B-4725-E D-4725-E F-4725-E AJ ,2 59,2 5, 7,2 71,2 3, 9, 74, 82, 84, 89, 45, Polar C18 B-4759-E D-4759-E F-4759-E AJ-953 F5 B-4723-E D-4723-E F-4723-E AJ-932 Biphenyl B-422-E D-422-E F-422-E AJ-927 XB-C18 B-449-E C-449-E D-449-E F-449-E AJ-878 C18 A-442-E B-442-E C-442-E D-442-E F-442-E AJ-878 C8 B-4497-E C-4497-E D-4497-E F-4497-E AJ-877 HILIC B-441-E C-441-E D-441-E F-441-E AJ-8772 Phenyl-Hexyl B-4495-E C-4495-E D-4495-E F-4495-E AJ mm CAMP Kinetex 2. μm SecurityGuard ULTRA (Part No. AJ-9) 11 5

6 MEGA LC UHPLC Luna HPLC Luna mega Phenomenex 2 Luna mega Phenomenex 5μm 3 μm 1. μm Intensity, cps.e5 5.e5 4.e5 3.e5 2.e5 1.e5 Conditions same for all columns Columns Luna mega 1.μm Polar C18 W AH 1.8 μm C Dimension 5 x 2.1mm Mobile Phase A Water with.1% Formic Acid B Acetonitrile with.1% Formic Acid Intensity, cps 1.2e 1.1e 1.e 9.e5 8.e5 7.e5.e5 5.e5 4.e5 3.e5 2.e5 1.e min Gradient Time (min) Luna mega 1.μm Polar C18 App ID Intensity, cps.e5 5.e5 4.e5 3.e5 2.e5 1.e5 % B Flow Rate.4mL/min Temperature 4C Detection MS/MS (SCIEX API 4 ) Luna mega 1.μm PS C18 App ID min 9 Intensity, cps 1.2e 1.1e 1.e 9.e5 8.e5 7.e5.e5 5.e5 4.e5 3.e5 2.e5 1.e min Sample Positive Mode (ESI) Acetyldeoxynivalenol 2. Aflatoxin G2 3. Aflatoxin G1 4. Aflatoxin B2 5. Aflatoxin B1. Aflatoxin M1 7. HT2 Toxin 8. T2 Toxin W AH 1.8 μm C18 App ID W AH 1.8 μm C18 App ID min Conditions for all columns Columns Luna mega 1. μm PS C18 W AH 1.8 μm C18 Dimension 1 x 2.1mm Mobile Phase A Water with.1% Formic Acid B Acetonitrile with.1% Formic Acid Phenomenex l WEB: Gradient Time (min) % B 5 Flow Rate.5mL/min Temperature 22C Detection MS/MS (SCIEX API 4) Sample 1. Lactic acid 2. Succinic acid 3. MMA 4. Glutaric acid 5. Ethylmalonic acid. Methyl succinate 7. Hippuric acid 8. Suberic acid 9. Sebacic acid

7 C18 Polar C18 PS C18 Polar Polar + + ph * ph * ph * 11 % 9 % 9 % USP L1 USP L1 USP L1 C18 UHPLC C18 1% C18 1% * ph ph 1.51 Luna 5 μm Minibore, MidBore (mm) SecurityGuard (mm) Phases 3 x x x x x 3. 1 x x 3. 4 x 2.* (1/pk) 34,4 3, 4, 43,2 3, 4, 52,8 32, 43, 45, 5, 54, 45, 5,, 4, Polar C18 A-4754-AN B-4754-AN D-4754-AN F-4754-AN B-4754-Y D-4754-Y F-4754-Y AJ-7 PS C18 A-4753-AN B-4753-AN D-4753-AN F-4753-AN B-4753-Y D-4753-Y F-4753-Y AJ mm 5 μm Analytical (mm) SecurityGuard (mm) Phases 5 x 4. 1 x x x 4. 4 x 3.* (1/pk) 3, 4, 43,2 53, 32, 45, 5, 54, 7, 4, Polar C18 B-4754-E D-4754-E F-4754-E G-4754-E AJ-71 PS C18 B-4753-E D-4753-E F-4753-E G-4753-E AJ mm 3 μm Minibore, MidBore (mm) SecurityGuard (mm) Phases 3 x x x x x 3. 1 x x 3. 4 x 2.* (1/pk) 47,2 48,8 53, 58,4 48,8 53, 58,4 32, 59, 1, 7, 73, 1, 7, 73, 4, Polar C18 A-47-AN B-47-AN D-47-AN F-47-AN B-47-Y D-47-Y F-47-Y AJ-7 PS C18 A-4758-AN B-4758-AN D-4758-AN F-4758-AN B-4758-Y D-4758-Y F-4758-Y AJ mm 3 μm Analytical (mm) SecurityGuard (mm) Phases 5 x 4. 1 x x x 4. 4 x 3.* (1/pk) 48,8 53, 58,4 5, 32, 1, 7, 73, 82, 4, Polar C18 B-47-E D-47-E F-47-E G-47-E AJ-71 PS C18 B-4758-E D-4758-E F-4758-E G-4758-E AJ mm 1. μm Minibore (mm) SecurityGuard ULTRA Phases 3 x x x x 2.1 3/pk 48,8 5,4 53,, 3, 1, 3, 7, 75, 45, Polar C18 A-4748-AN B-4748-AN D-4748-AN F-4748-AN AJ-955 PS C18 A-4752-AN B-4752-AN D-4752-AN F-4752-AN AJ-958 C18 A-4742-AN B-4742-AN D-4742-AN F-4742-AN AJ mm SecurityGuard ULTRA (Part No. AJ-9) 11 * SecurityGuard (Part No. KJ-4282) μm Microbore (mm) Phases 5 x 1. 1 x x 1. 5,4 53,, 3, 7, 75, Polar C18 B-4748-A D-4748-A F-4748-A C18 B-4742-A D-4742-A F-4742-A CAMP 7

8 Chiral LC Columns 8 3 % FF Lux i-amylose-1 i-cellulose-5 THFDMSMTBE DMS 2 Lux i-amylose-1 CH 3 Lux i-cellulose-5 CI H 3 C CI NH NH Lux i-amylose-1 3,5- Lux i-cellulose-5 3,5- Lux H 3 C CH 3 NH H 3 C NH CI H 3 C CH 3 NH CH 3 CI NH CH 3 CI H 3 C NH Cellulose--CNH Amylose-1 3,5- Amylose Cellulose-1 3,5- Cellulose Cellulose-3 4- Cellulose Phenomenex l WEB:

9 Lux 5 μm i-cellulose-5 a = 1.33 Lux 5 μm i-amylose-1 a = 1.77 APP ID min min 5 μm a = μm a = 1.73 APP ID 2371 APP ID APP ID min min Conditions for both columns: Dimensions: 25 x 4. mm Mobile Phase:.1 % Ethylamine in MTBE /.1 % Ethylamine in Methanol (9:4) Flow Rate: 1 ml/min Detection: 254 nm Temperature: Ambient Sample: Chlorthalidone Conditions for both columns: Dimensions: 25 x 4. mm Mobile Phase:.1 % DEA in Hexane /.1 % DEA in IPA (9:1) Flow Rate: 1 ml/min Detection: 27 nm Temperature: Ambient Sample: Tramadol CAMP 3μmMinibore,MidBore,Analytical(mm) SecurityGuard(mm) 5x2. 15x2. 1x3. 15x3. 5x4. 1x4. 15x4. 25x4. 4x2.* 4x3.* 89, 17,1 98, 17,1 89, 98, 17,1 11,2 33,(1/pk) 33,(1/pk) 128, 153, 14, 153, 128, 14, 153, 1, 42,(1/pk) 42,(1/pk) i-amylose-1 B-471-B F-471-B D-471-Y F-471-Y B-471-E D-471-E F-471-E G-471-E AJ-84 AJ-841 i-cellulose-5 B-4755-B F-4755-B D-4755-Y F-4755-Y B-4755-E D-4755-E F-4755-E G-4755-E AJ-831 AJ , 17,1 98, 17,1 89, 98, 17,1 11,2 33,(1/pk) 33,(1/pk) 128, 153, 14, 153, 128, 14, 153, 1, 42,(1/pk) 42,(1/pk) Cellulose-1 B-4458-B F-4458-B D-4458-Y F-4458-Y B-4458-E D-4458-E F-4458-E G-4458-E AJ-842 AJ-843 Cellulose-2 B-445-B F-445-B D-445-Y F-445-Y B-445-E D-445-E F-445-E G-445-E AJ-8398 AJ-83 Cellulose-3 B-4492-B F-4492-B D-4492-Y F-4492-Y B-4492-E D-4492-E F-4492-E G-4492-E AJ-821 AJ-822 Cellulose-4 B-449-B F-449-B D-449-Y F-449-Y B-449-E D-449-E F-449-E G-449-E AJ-82 AJ-827 Amylose-1 B-4729-B F-4729-B D-4729-Y F-4729-Y B-4729-E D-4729-E F-4729-E G-4729-E AJ-9337 AJ-933 Amylose-2 B-4471-B F-4471-B D-4471-Y F-4471-Y B-4471-E D-4471-E F-4471-E G-4471-E AJ-8471 AJ ~3. mm 3.2~8. mm 5μmMinibore,Analytical(mm) SecurityGuard(mm) 5x2. 5x4. 1x4. 15x4. 25x4. 4x2.* 4x3.* 8,5 8,5 89, 98, 17,1 33,(1/pk) 33,(1/pk) 115, 115, 128, 14, 153, 42,(1/pk) 42,(1/pk) i-amylose-1 B-472-B B-472-E D-472-E F-472-E G-472-E AJ-84 AJ-841 i-cellulose-5 B-475-E D-475-E F-475-E G-475-E AJ-831 AJ-832 8,5 8,5 89, 98, 17,1 33,(1/pk) 33,(1/pk) 115, 115, 128, 14, 153, 42,(1/pk) 42,(1/pk) Cellulose-1 B-4459-B B-4459-E D-4459-E F-4459-E G-4459-E AJ-842 AJ-843 Cellulose-2 B-4457-B B-4457-E D-4457-E F-4457-E G-4457-E AJ-8398 AJ-83 Cellulose-3 B-4493-B B-4493-E D-4493-E F-4493-E G-4493-E AJ-821 AJ-822 Cellulose-4 B-4491-B B-4491-E D-4491-E F-4491-E G-4491-E AJ-82 AJ-827 Amylose-1 B-4732-B B-4732-E D-4732-E F-4732-E G-4732-E AJ-9337 AJ-933 Amylose-2 B-4472-B B-4472-E D-4472-E F-4472-E G-4472-E AJ-8471 AJ ~3. mm 3.2~8. mm * SecurityGuard(Part No. KJ-4282)11 9

10 Hydraulic Piston Compression Axia 21.2 mm 5 μm Kinetex Axia Packed (mm) SecurityGuard PREP Phases 5 x x x x x 21.2* 157, 235,2 29, 321, 197, 294, 337, 42, 38, (1/pk) EV C18 B-433-P-AX D-433-P-AX F-433-P-AX G-433-P-AX AJ ,2 213, 244,8 292, 179, 27, 3, 35, 38, (1/pk) Biphenyl B-427-P-AX D-427-P-AX F-427-P-AX G-427-P-AX AJ-9272 XB-C18 B-45-P-AX D-45-P-AX F-45-P-AX G-45-P-AX AJ-9145 C18 B-41-P-AX D-41-P-AX F-41-P-AX G-41-P-AX AJ-9145 C8 B-48-P-AX D-48-P-AX F-48-P-AX G-48-P-AX AJ-925 Phenyl-Hexyl B-43-P-AX D-43-P-AX F-43-P-AX G-43-P-AX AJ-9147 HILIC D-4-P-AX F-4-P-AX G-4-P-AX AJ mm SecurityGuard 5 μm Luna mega Axia Packed (mm) PREP (mm) Phases 15 x x x 21.2* 24, 3,4 33, 383, 38, (1/pk) Polar C18 F-4754-P-AX G-4754-P-AX AJ-73 PS C18 F-4753-P-AX G-4753-P-AX AJ mm * SecurityGuard PREP (Part No. AJ-8223) 7 () CAMP Axia 1 Phenomenex l WEB:

11 ULTRA UHPLC Column Protection UHPLC 3 μm UHPLC mm 137 MPa SecurityGuard ULTRA AJ-9 SecurityGuard ULTRA Cartridge Holder 1/pk AJ mm 3. mm 4. mm SecurityGuard ULTRA CAMP } SecurityGuard ULTRA HPLC Column Protection HPLC HPLC 3 μm HPLC 2.8. mm 24 MPa CAMP SecurityGuard Standard KJ-4282 SecurityGuard Guard Cartridge Kit PEEK 2 22 PEEK32 KJ x 2. mm 4 x 3. mm SecurityGuard 11

12 Bio Core-Shell Technology Aeris WIDEPRE 3. μm > 1 kda < 1 kda 3XB-C18XB-C8C4 3. μm UHPLC HPLC HPLC Aeris PEPTIDE 3. μm XB-C18 UHPLC LC Si XB- C18 Si XB- C8 C4 Aeris WIDEPRE 3. μm XB-C18 Aeris PEPTIDE 1.7 μm XB-C18 Aeris PEPTIDE 2. μm XB-C18 PEG Aeris WIDEPRE 3. μm XB-C8 Aeris PEPTIDE 5 μm XB-C18 Aeris PEPTIDE Aeris WIDEPRE 3. μm C4 XB-C μm2. μm3. μm 5 μm (μm) (μm) (μm) ph Aeris WIDEPRE C MPa Aeris PEPTIDE C 1 MPa Aeris PEPTIDE C 1 MPa Aeris PEPTIDE C MPa Aeris PEPTIDE C MPa 12 Phenomenex l WEB:

13 4. mm Aeris PEPTIDE 1.7 μm Minibore (mm) SecurityGuard ULTRA * Phase 5 x x x 2.1 3/pk 71,2 75,2 79,2 3, 89, 94, 99, 45, XB-C18 B-45-AN D-45-AN F-45-AN AJ mm Aeris PEPTIDE 2. μm Minibore (mm) SecurityGuard ULTRA * Phase 5 x x x x 2.1 3/pk 8, 71,2 75,2 8,8 3, 85, 89, 94, 11, 45, XB-C18 B-455-AN D-455-AN F-455-AN G-455-AN AJ mm Aeris PEPTIDE 2. μm MidBore and Analytical (mm) SecurityGuard ULTRA * Phase 15 x x x 4. 3/pk 3/pk 75,2 75,2 8,8 3, 3, 94, 94, 11, 45, 45, XB-C18 F-455-Y F-455-E G-455-E AJ-8947 AJ mm 4. mm Aeris PEPTIDE 3. μm Minibore (mm) SecurityGuard ULTRA * Phase 5 x x x x 2.1 3/pk 5, 58,4 3,2,4 3, 7, 73, 79, 83, 45, XB-C18 B-457-AN D-457-AN F-457-AN G-457-AN AJ mm Aeris PEPTIDE 3. μm Analytical (mm) SecurityGuard ULTRA * Phase 5 x 4. 1 x x x 4. 3/pk 5, 58,4 3,2,4 3, 7, 73, 79, 83, 45, XB-C18 B-457-E D-457-E F-457-E G-457-E AJ mm Aeris PEPTIDE 5 μm Analytical Scout and Semi-Prep (mm) SecurityGuard SecurityGuard ULTRA * SemiPrep ** Phase 15 x x x x 1. 3/pk 1 x 1 48,8 49, 3, 1, 2, 12, 195, 45, XB-C18 F-432-E G-432-E F-432-N G-432-N AJ-894 AJ mm 1 mm SecurityGuard Aeris PEPTIDE 5 μm Axia Packed (mm) PREP Phase 15 x x x , 299,2 315, 374, XB-C18 F-432-P-AX G-432-P-AX AJ mm Aeris WIDEPRE 3. μm Minibore (mm) SecurityGuard ULTRA * Phases 5 x x x x 2.1 3/pk 5,8 59,2 4, 7,2 3, 71, 74, 8, 84, 45, XB-C18 B-4482-AN D-4482-AN F-4482-AN G-4482-AN AJ-8783 XB-C8 B-4481-AN D-4481-AN F-4481-AN G-4481-AN AJ-8785 C4 B-448-AN D-448-AN F-448-AN G-448-AN AJ mm Aeris WIDEPRE 3. μm Analytical (mm) SecurityGuard ULTRA * Phases 1 x x x 4. 3/pk 59,2 4, 7,2 3, 74, 8, 84, 45, XB-C18 D-4482-E F-4482-E G-4482-E AJ-879 XB-C8 D-4481-E F-4481-E G-4481-E AJ-8771 C4 D-448-E F-448-E G-448-E AJ mm * SecurityGuard ULTRA Part No. AJ-911 **SecurityGuard SemiPrep Part No. AJ , SecurityGuard PREP Part No. AJ-82237, CAMP 13

14 生体分子用の超高分離能 サイズ排除 (GFC/SEC) カラム Ultra-High Efficiency GFC/SEC 5% FF 極めて高い理論段数の 3 μm HPLC 用 優れたコストパフォーマンス 高い不活性度による低い吸着性 Yarra 3 μm の優れた分離能 mau Phenomenex Yarra 3 μm SEC-3 5 Conditions for all columns: Columns: Yarra 3 μm SEC-3 Tカラム 5 μm SEC-3 Dimensions: 3 x 7.8 mm Mobile Phase: 5 mm Sodium Phosphate ph.8 /.3 M Sodium Chloride Flow Rate: 1 ml/min Temperature: Ambient Detection: 22 nm Sample: 1. IgM 2. Thyroglobin (99 kda) 3. Beta Amylase 4. valbumin (44 kda) 5. Myoglobin (17 kda). Uridine 2 4 T社 Tカラム 5 μm SEC 分離度が向上 App ID min 比類のない再現性 mau 35 3つの個別のバッチを 重ねて表示 カラム間とバッチ間での 再現性が非常に高い パフォーマンス Columns: Dimensions: Part No.: Mobile Phase: Flow Rate: Temperature: Detection: Sample: 2 15 APP ID Yarra 3 μm SEC-3 3 x 7.8 mm H-4513-K 1 mm Sodium Phospate ph % NaN3 1 ml/min Ambient 28 nm 1. Thyroglobin (99 kda) 2. IgA (3 kda) 3. IgG (15 kda) 4. valbumin (44 kda) 5. Myoglobin (17 kda). Uridine min 極めて高い不活性度 最小限の吸着性 他社のGFCカラムと 比べて極めて少ない タンパク質吸着 Tカラム 2 Injection 2 Injection 1 14 Phenomenex l 8 WEB: APP ID min Injection Conditions for all columns: Columns: Yarra 3 μm SEC-3 Tカラム 5 μm SEC-3 Dimensions: 3 x 7.8 mm Mobile Phase: 5 mm Sodium Phosphate ph 5./.1 M Sodium Sulfate Flow Rate: 1 ml/min Temperature: Ambient Detection: 28 nm Sample: 1. IgG Dimer 2. IgG Monomer 3. Albumin Injection 2 IgG二量体の ピークが吸着 APP ID 219 Yarra 8 1 min

15 Yarra 3 μm SEC-29 Number of Plates per Column Injections App ID Retention Time (min) Column: Yarra 3 μm SEC-2 Dimensions: 3 x 7.8 mm Part No.: H-4512-K Guard Cartridge: AJ-4487 Guard Holder: KJ-4282, SecurityGuard Guard Cartridge Kit Mobile Phase: 1 mm Sodium Phosphate in Water ph % NaN 3 Flow Rate: 1 ml/min Detection: 28 nm Filter: AF , Phenex-PES 28 mm Syringe Filters.45 μm, Non-Sterile, Luer/Slip Vial: AR , Verex Vial Kit, 9 mm, 2 ml Clear 33 w/ Patch + PTFE/Silicone, preslit Sample: 1. Thyroglobulin (9 kda) 2. IgA (3 kda) 3. IgG (15 kda) 4. valbumin (44 kda) 5. Myoglobin (17 kda). Uridine (Da) Under M GnHCI Under.5 % SDS Native 4 5,-7, 3 1,-15, 2 5-1, , 4 15,-5, 3 5,-1, 4 15,-1,5, 3 5,-7, 2 1,-3, Molecular Weight (Daltons) SEC-2 SEC-3 SEC-4 (μm) (Å) (Da) 1K-3K 5K-7K 15K-15K ph (psi) (psi) (3 x 7.8mm) 48 (3 x 7.8mm) 38 (3 x 7.8mm) (ml/min) Protein MW (da) 1 SEC-2 SEC-3 SEC Elution Volume (ml) Yarra 3 μmsem App ID 238 Yarra 3 μm SEC(mm) Narrow Bore Analytical Analytical SecurityGuard (mm) Phases 3 x x x x 3.* 124, 13,5 115, 32, (1/pk) 248, 27, 23, 4, (1/pk) Yarra 3μm SEC-2 Yarra 3μm SEC-3 Yarra 3μm SEC-4 H-4512-E F-4512-K H-4512-K AJ-4487 H-4513-E F-4513-K H-4513-K AJ-4488 H-4514-E H-4514-K AJ mm * SecurityGuard (Part No. KJ-4282) CAMP 15

16 rganic GPC/SEC (GPC/SEC) 5 μm 1 μm 4.mm 14 C Da 1 3 K 5 K 1 K 15 K 1 K 75 K 5 K 5 K 1 K 1 K K 1 K 1 1 K 5 % FF Phenogel 5 Å 1 Å 5 Å 1 3 Å 1 4 Å 1 5 Å 1 Å Linear(2) (SDVB) 5 μm 1 μm 5 Å 1 Å 15 psi (1 MPa) 14C 5 μm 45 p/m** 1 μm 35 p/m** 4.mm ID.35mL/min 7.8mm ID 1.mL/min 21.2mm ID 7.mL/min * THF ** 3 x 7.8mm Phenogel CAMP (ml) 5 μm Analytical (mm) SecurityGuard (mm) (Da) 3 x x 3.* 5 x , 34,4 (3/pk) 3, 25, 43, (3/pk), 5 Å 13 K H-441-K AJ B-288-K 1 Å 5 K H-442-K AJ B-288-K 5 Å 1 K15 K H-443-K AJ B-288-K 1 3 Å 1 K75 K H-444-K AJ B-288-K 1 4 Å 5 K5 K H-445-K AJ B-288-K 1 5 Å 1 K1 K H-44-K AJ B-288-K 1 Å K1 K H-447-K AJ B-288-K 3 x x 3.* 5 x , 34,4 (3/pk) 3, 25, 43, (3/pk), Linear(2) 11 K H-3259-K AJ B-288-K 3.28.mm 5 μm Narrow Bore (NB) (mm) SecurityGuard (mm) (Da) 3 x 4. 4 x 3.* 3 x 4. 12, 34,4 (3/pk) 27,5 24, 43, (3/pk) 55, 5 Å 13 K H-441-E AJ A-288-E 1 Å 5 K H-442-E AJ A-288-E 5 Å 1 K15 K H-443-E AJ A-288-E 1 3 Å 1 K75 K H-444-E AJ A-288-E 1 4 Å 5 K5 K H-445-E AJ A-288-E 1 5 Å 1 K1 K H-44-E AJ A-288-E 1 Å K1 K H-447-E AJ A-288-E 3 x 4. 4 x 3.* 3 x 4. 12, 34,4 (3/pk) 27,5 24, 43, (3/pk) 55, Linear(2) 11 K H-3259-E AJ A-288-E 3.28.mm * SecurityGuard (Part No. KJ-4282) 11 1 Phenomenex l WEB:

17 Ion Exclusion LC RCM-Monosaccharide (L19 packing)* RHM-Monosaccharide (L17 packing)* RAM-Carbohydrate RS-ligosaccharide RN-ligosaccharide RPM-Monosaccharide (L34 packing)* RNM-Carbohydrate (L54 packing)* RA-rganic Acid (L22 packing)* RFQ-Fast Acid RKP-Potassium RCU-USP Sugar Alcohols (L19 packing)* * (USP) 8% cross-linked resin CALCIUM ionic form 8% cross-linked resin HYDRGEN ionic form 8% cross-linked resin SILVER ionic form 4% cross-linked resin SILVER ionic form 4% cross-linked resin SDIUM ionic form 8% cross-linked resin LEAD ionic form 8% cross-linked resin SDIUM ionic form 8% cross-linked resin HYDRGEN ionic form 8% cross-linked resin HYDRGEN ionic form 8% cross-linked resin PTASSIUM ionic form 8% cross-linked resin CALCIUM ionic form Rezex 18 (Dp) USP ph CAMP 1 mm SecurityGuard (mm) (mm) 4 x 3.* (mm) 1/pk RCM-Monosaccharide F-13-K 8% Calcium 15 x B-13-K 5 x 7.8 AJ RCM-Monosaccharide H-13-K 8% Calcium 3 x B-13-K 5 x 7.8 AJ RHM-Monosaccharide H-132-K 8% Hydrogen 3 x B-132-K 5 x 7.8 AJ H-131-K RAM-Carbohydrate 8% Silver 3 x AJ P-133-N RS-ligosaccharide 4% Silver 2 x R-133-N x P-137-N RN-ligosaccharide 4% Sodium 2 x R-137-N x H-135-K 8% Lead 3 x B-135-K 5 x 7.8 AJ RPM-Monosaccharide (for USP procedure) D-135-K 8% Lead 1 x B-135-K 5 x AJ H-13-K RNM-Carbohydrate 8% Sodium 3 x B-13-K 5 x F-138-E RA-rganic Acid 8% Hydrogen 15 x 4. 1 AJ G-138-E RA-rganic Acid 8% Hydrogen 25 x AJ F-138-K RA-rganic Acid 8% Hydrogen 15 x B-138-K 5 x 7.8 AJ H-138-K RA-rganic Acid 8% Hydrogen 3 x B-138-K 5 x 7.8 AJ RKP-Potassium H-3252-K 8% Potassium 3 x D-223-K RFQ-Fast Acid 8% Hydrogen 1 x B-223-K 5 x 7.8 AJ RCU-USP Sugar Alcohols G-13-D 8% Calcium 25 x A-13-D 3 x AJ mm * SecurityGuard (Part No. KJ-4282) 11 17

18 GC Columns GC ZB-WAXPLUS ZB-WAXPLUS (PEG) GC Zebron ZB-WAXPLUS PLARITY MS CERTIFIED min App ID USP PHASE G14, G15, G1, G2, G39, G47 TEMP LIMITS ( C) 2 to 25/2* Conditions same for both columns: Dimension: Injection: Carrier Gas: ven Program: Detector: Zebron ZB-WAXPLUS (m) (mm) (μm) C 18 Phenomenex l WEB: Sample: 1. Methyl Formate 2. Acetone 3. Ethyl Acetate 4. Methyl Ethyl Ketone 5. Methanol. 2-Methyl-2-propanol 7. Methylene Chloride 8. Benzene 9. Ethanol 39, 31,2 3, 28,8 42,4 33,92 5, 4, 59, 47,2,4 53,12 5, 52, 3, 5,4 7, 53, 75,, 134, 17,8 18, 8,4 113, 9,4 138,9 111,12 115, 92, 123, 98,4 App ID Butanol 11. Toluene 12. n-propanol 13. Ethyl Benzene 14. p-xylene 15. m-xylene 1. 1-Butanol 17. o-xylene Zebron H H C C H H Agilent Restek SGE Supelco CAMP

19 Phenomenex Zebron GC Zebron Phase Zebron Composition Restek Agilent Supelco SGE V ZB-1 ZB-1PLUS ZB-1HT Inferno ZB-1XT SimDist ZB-5 ZB-5PLUS ZB-5HT Inferno ZB-5ms ZB-5MSPLUS ZB-SemiVolatiles ZB-35 ZB-35HT Inferno ZB-5 ZB-24 ZB-171 ZB-171P ZB-23 1 % Dimethylpolysiloxane 1 % Dimethylpolysiloxane 1 % Dimethylpolysiloxane 1 % Dimethylpolysiloxane Rtx -1, Rtx-1PNA, Rtx-1 F&F Rtx-1ms, Rxi -1ms DB -1, DB-2887, DB-1 EVDX, HP-1, HP-11, HP-PNA,Ultra 1, CP-Sil 5 CB DB-1ms, DB-1ms Ultra Inert, HP-1ms, HP-1ms Ultra Inert, CP-Sil 5 CB MS, VF-1ms SPB -1, SPB-1 TG, SE-3, MET-1, SPB-1 Sulfur, SPB- HAP MDN-1, Equity -1 Rxi-1HT DB-1ht, CP-SimDist Petrocol 2887 MXT -1HT SimDist, MXT-1, MXT-1 Sim- Dist, MXT-2887 CP-SimDist UltiMetal, CP-Sil 8 CB UltiMetal, BPX1-SimD, DB-HT SimDis, DB-PS1, DB-PS % Phenyl 95 % Dimethylpolysiloxane Rtx-5 DB-5, HP-5, Ultra 2, HP-PAS-5, CP-Sil 8 CB 5 % Phenyl 95 % Dimethylpolysiloxane 5 % Phenyl 95 % Dimethylpolysiloxane 5 % Phenyl-Arylene 95 % Dimethylpolysiloxane Rtx-5ms, Rxi-5ms, Rtx-5Amine Rxi-5HT, Rtx-5HT Stx -5HT, XTI -5HT Rtx-5Sil MS, Rxi-5Sil MS MDN-5, SPB-5, PTE-5, SE-54, PTA-5, Equity-5, Sac-5 DB-5, HP-5ms, HP-5msi MDN-5S DB-5ht, VF-5ht HT-5 DB-5ms, DB-5.25, DB-5ms EVDX, VF-5ms, CP-Sil 8 CB MS 5 % Phenyl-Arylene 95 % Dimethylpolysiloxane Rxi-5Sil MS DB-5ms Ultra Inert, HP-5ms Ultra Inert, DB-5ms, VF-5ms 5 % Phenyl-Arylene 95 % Dimethylpolysiloxane 35 % Phenyl 5 % Dimethylpolysiloxane 35 % Phenyl 5 % Dimethylpolysiloxane Rxi-5Sil MS, Rxi-5ms Rtx-35, Rtx-35ms DB-5ms Ultra Inert, HP-5ms Ultra Inert DB-35, DB-35ms, HP-35, HP-35ms DB-17, DB-17HT, DB-17ms, 5 % Phenyl 5 % Dimethylpolysiloxane Rtx-5 DB-17 EVDX, HP-5+, CP-Sil 24 CB % Cyanopropyl phenyl 94 % Dimethylpolysiloxane Rtx-131, Rtx-24 DB-131, DB-24, DB-VRX, HP-VC, CP-131, CP-Select 24 CB SLB -5ms SLB-5ms MDN-35, SPB-35, SPB-8 Phenomenex Exclusive SP-225, SPB-17, SPB-5 14 % Cyanopropyl phenyl 8 % Dimethylpolysiloxane Rtx-171 DB-171, CP-Sil 19 CB SPB-171, Equity % Cyanopropyl phenyl 8 % Dimethylpolysiloxane 5 % Cyanopropyl 5 % Methylpolysiloxane DB-171P Rtx-233 DB-23 SP -233 ZB % Cyanopropyl 12 % Arylpolysiloxane Rt -25 CP-Sil 88, HP-88 SP-25 ZB-FAME High Cyanopropyl CP-Sil 88 SP -25, SP-238 ZB-WAX Polyethylene Glycol Rtx-WAX, Famewax, Stabilwax-DB DB-WAXetr, HP-INNWax, CP-Wax 57 CB BP1, BP1-PNA, BPX1-SimD SolGel-1ms BP5, BPX5 BPX35, BPX8 BPX5 V-1 V-5 V-11 V-17 SPB-131, SPB-24 BP24 V-24 BP1 MET-Wax, megawax SolGel-WAX V-171 ZB-WAXPLUS Polyethylene Glycol Stabilwax DB-WAX, CAM, HP-2M, Carbowax 2M, CP-Wax 52 CB SUPELCWAX 1 BP2 Carbowax 2M ZB-FFAP ZB-MultiResidue -1 ZB-MultiResidue-2 ZB-CLPesticides-1 Nitroterephthalic Acid Modified Polyethylene Glycol Proprietary Proprietary Proprietary Stabilwax-DA Rtx-CLPesticides, Stx-CLPesticides Rtx-CLPesticides2, Stx-CLPesticides2 Rtx-CLPesticides, Stx-CLPesticides DB-FFAP, HP-FFAP, CP-Wax 58 FFAP CB, CP-FFAP CB ZB-CLPesticides-2 Proprietary Rtx-CLPesticides2, Stx-CLPesticides2 ZB-PAH Proprietary Rxi-PAH DB-EUPAH ZB-XLB Proprietary Rtx-XLB DB-XLB, VF-XMS MDN-12 ZB-XLB-HT Inferno Proprietary Phenomenex Exclusive ZB-Drug-1 Proprietary Phenomenex Exclusive ZB-BAC-1 Proprietary Rtx-BAC1 DB-ALC1 ZB-BAC-2 Proprietary Rtx-BAC2 DB-ALC2 ZB-Bioethanol Proprietary Phenomenex Exclusive Phenomenex Nukol, SPB-1 BP21 V

20 Polymeric SPE (SPE) Strata-X / Strata-XL Strata-X-C / Strata-XL-C Strata-X-CW / Strata-XL-CW Strata-X-A / Strata-XL-A Strata-X-AW / Strata-XL-AW (pk a 8-1) (pk a >1) (pk a 2-4) (pk a <2) 33μm 85 Å 1μm 3 Å Phase (Tube) Strata-X Strata-X-C Strata-X-CW Strata-X-A Strata-X-AW 3 mg 1 ml (1/box) 8B-S1-TAK mg 3 ml (5/box) 8B-S1-UBJ 2 mg 3 ml (5/box) 8B-S1-FBJ 5 mg ml (3/box) 8B-S1-HCH Strata-X 9- SPE B-S29-TAK 8B-S35-TAK 8B-S123-TAK 8B-S38-TAK B-S29-UBJ 8B-S35-UBJ 8B-S123-UBJ 8B-S38-UBJ B-S29-FBJ 8B-S35-FBJ 8B-S123-FBJ 8B-S38-FBJ B-S29-HCH 8B-S35-HCH 8B-S123-HCH 8B-S38-HCH Phase (9-Well Plate) Strata-X Strata-X-C Strata-X-CW Strata-X-A Strata-X-AW 1 mg 2 Plates/Box 8E-S1-AGB 3 mg 2 Plates/Box 8E-S1-TGB E-S29-AGB 8E-S35-AGB 8E-S123-AGB 8E-S38-AGB E-S29-TGB 8E-S35-TGB 8E-S123-TGB 8E-S38-TGB M-S1-4GA 8M-S29-4GA 8M-S35-4GA 8M-S123-4GA 8M-S38-4GA Strata-X 33 μm Polymeric Reversed Phase Microelution 9-Well Plate 2mg/well Strata-X-C 33 μm Polymeric Strong Cation-Exchange Microelution 9-Well Plate 2mg/well Strata-X-CW 33 μm Polymeric Weak Cation-Exchange Microelution 9-Well Plate 2mg/well Strata-X-A 33 μm Polymeric Strong Anion-Exchange Microelution 9-Well Plate 2mg/well Strata-X-AW 33 μm Polymeric Weak Anion-Exchange Microelution 9-Well Plate 2mg/well 1/pk /pk /pk /pk /pk CAMP 2 Phenomenex l WEB:

21 Solid Phase Extraction (SPE) (μm) (m 2 /g) (%) (meq/g) C18-E trifunctional Yes C18-U trifunctional No C18-T trifunctional Yes C trifunctional Yes Phenyl trifunctional Yes CN trifunctional No NH trifunctional No 1.3 Silica (Si-1) SCX trifunctional No.9 WCX trifunctional No.8 SAX trifunctional No.9 Screen-C GF proprietary trifunctional Screen-C proprietary trifunctional Screen-A proprietary trifunctional ABW FL-PR (Florisil ) EPH (Extractable Petroleum Hydrocarbon) 2 7 proprietary. AL-N (Alumina-Neutral) SDB-L Eco-Screen proprietary proprietary proprietary Melamine proprietary proprietary proprietary proprietary PAH proprietary proprietary proprietary proprietary Phase Tube C18-E Tube 1 mg 1 ml (1/box) 8B-S1-EAK 2 mg 3 ml (5/box) 8B-S1-FBJ 5 mg ml (3/box) 8B-S1-HCH Phase Tube Phenyl Tube 1 mg 1 ml (1/box) 8B-S-EAK 2 mg 3 ml (5/box) 8B-S-FBJ 5 mg ml (3/box) 8B-S-HCH mg 3 ml (5/box) 8B-S12-FBJ mg 3 ml (5/box) 8B-S9-FBJ Silica (Si-1) 5 mg ml (3/box) 8B-S12-HCH NH 2 5 mg ml (3/box) 8B-S9-HCH Tube 1 g ml (3/box) 8B-S12-JCH Tube 1 g ml (3/box) 8B-S9-JCH mg 3 ml (5/box) 8B-S7-FBJ mg ml (3/box) 8B-S13-HCH Cyano (CN) 5 mg ml (3/box) 8B-S7-HCH Florisil 1 g ml (3/box) 8B-S13-JCH g ml (3/box) 8B-S7-JCH g 12 ml (2/box) 8B-S13-JEG CAMP 21

22 Phospholipid Removal Solutions 99%LC-MS Phree 1 Solvent Shielding Technology Phree Phree Phree 9-8B-S133-TAK Phree Phospholipid Removal 1mL Tube 1/box E-S133-TGB Phree Phospholipid Removal 9-Well Plates 2/box CAMP β-glucuronidase Removal Products β- β-1 LC 1 β-gone 9-1minute HPLC/UHPLC β-gone β- 9-8B-S139-TAK 1mL Tubes Recombinant Enzyme 1/Box 2 1 8B-S322-DAK 1mL Tubes Non-Recombinant Enzyme 1/Box 2 1 8E-S139-TGA 9-Well Plate Recombinant Enzyme 1/Box 2 1 8E-S322-DGA 9-Well Plate Non-Recombinant Enzyme 1/Box CAMP 22 Phenomenex l WEB:

23 simplified liquid extraction 液液抽出より速くて簡単 そして信頼性も高い前処理法 2% FF エマルジョンによる望ましくない結果を防ぐ サンプル中の夾雑物を除去 自動化することによって生産性を向上 簡易化液抽出 (SLE) Novum SLE (Simplified Liquid Extraction) は時間がかかる工程をなくし 分析対象物の損失のリスクを減らすことによっ てスループットを直ちに向上します さらに時間を短縮する必要がある場合 Novum SLE は容易に自動化できるので 迅 速なハンズフリー機能でサンプルをクリーンアップできます 液液抽出 時間と労力を費やす 簡易化液抽出 迅速で簡単 従来の 液液抽出 推定所要時間 約25分 Novum 簡易化液抽出 (SLE) 推定所要時間 15分以下 1. サンプルを緩衝液または水で 1. サンプルを緩衝液または水で 1:1 に希釈し 抽出溶媒を 加えます 1:1 に希釈し Novum SLE に ロードしてから吸引します 2 15秒程度 simplified liquid extraction novum simplified liquid extraction novum 2. 1分間撹拌します 2. 5分間待ちます 3. 1分間遠心分離にかけます 3. 溶出溶媒を入れて 自然落下で溶出 simplified liquid extraction 15 novum simplified liquid extraction novum 19 2 させます 1秒程度バキューム 吸引 して完全に溶出します 4. 上澄みを捨てるか 凍らせます ハイスループットクリーンアップ用に高速化 自動化できるメソッドです エマルジョンによる分析対象物の損失の心配はいりません 9-ウェルプレート 製品番号 製品名 入り数 希望小売価格 キャンペーン価格 8E-S138-FGA Novum SLE MINI 9-Well Plate 1/pk 2, 円 2,8 円 8E-S138-5GA Novum SLE MAX 9-Well Plate 1/pk 33, 円 2,4 円 シリンジ型チューブ 製品番号 8B-S138-FAK 8B-S138-5BJ 8B-S138-JCH 製品名 Novum SLE 1 cc tubes Novum SLE 3 cc tubes Novum SLE cc tubes 入り数 1/pk 5/pk 3/pk 希望小売価格 25, 円 14, 円 11, 円 キャンペーン価格 2, 円 11,2 円 8,8 円 8B-S138-KDG Novum SLE 12 cc tubes 2/pk 9, 円 7,2 円 キャンペーン期間 218年3月2日 金 まで キャンペーンコード CAMP ご発注の際は キャンペーンコードを必ずご明示ください ご明示のない場合 キャンペーン適用となりませんのでご注意ください novum novum simplified liquid extr action simplified liquid extraction 掲載価格は税抜です 仕様 外観 価格は予告なく 変更する場合がございますので ご了承ください 23

24 t: +353 () f: t: +1 (31) f: +1 (31) t: +44 () f: +44 () t: f: t: +91 () f: +91 () t: +1 () f: +1 () t: +43 () f: +43 () t: +31 () f: +31 () t: +1 (8) f: +1 (31) t: f: swissinfo@phenomenex.com t: +4 () f: nordicinfo@phenomenex.com t: f: esp info@phenomenex.com t: f: +8 () phen@agela.com t: f: nordicinfo@phenomenex.com t: +49 () f: +49 () anfrage@phenomenex.com t: +4 () f: +4 () nzinfo@phenomenex.com t: f: nordicinfo@phenomenex.com t: +358 () f: nordicinfo@phenomenex.com t: +1 (8) f: +1 (31) info@phenomenex.com t: +33 () f: +33 () franceinfo@phenomenex.com t: +32 () () t: +32 () () f: +31 () beinfo@phenomenex.com t: f: tecnicomx@phenomenex.com t: +31 () f: +31 () nlinfo@phenomenex.com t: +1 (31) f: +1 (31) info@phenomenex.com 25% FF LC GC Happy New year! Phenomenex Phenomenex Luna Kinetex Strata Phenomenex Aeris Axia Lux MidBore Novum Phenogel Phree Rezex SecurityGuard Solvent Shielding Technology Yarra Zebron Inferno 1PLUS 5PLUS 5MSPLUS WAXPLUS MultiResidue β-gone Phenomenex API 4 AB SCIEX Pte. Ltd. AB SCIEX Axia Phenomenex Kinetex EV Phenomenex SecurityGuard Phenomenex 1232 SecurityGuard SemiPrepPREPULTRA Strata-X Phenomenex Phenomenex Inc. All rights reserved. 24 Phenomenex l WEB: BR _I_jp_Ja