ORYZA OIL & FAT CHEMICAL CO., LTD. WHITE JELLY FUNGUS EXTRACT -P () Ver. 1.3SJ
Ver. 1.3SJ 1. 3000 ph 1
Ver. 1.3SJ 1. 1 2. 3 2-1. 2-2. 2-3. 2-4. SOD 3. 7 3-1. in vitro 3-2. in vivo 3-3. in vivo 3-4. in vivo 4. 10 4-1. in vivo 4-2. in vivo 4-3. in vivo 4-4. 5. 13 5-1. in vitro 5-2. in vitro 5-3. 6. 15 6-1. 6-2. 7. 16 7-1. in vitro 7-2. in vivo 8. 18 8-1. in vivo 8-2. 9. 19 9-1. in vivo 9-2. 10.16. 20 2
Ver. 1.3SJ 2. 2-1. 3
Ver. 1.3SJ 4 2-2. α-1,3- D-D- D- 9143moler 17.6 % 80100 D-L- Man Man Man Man Man Man Man Man Man GluA GluA GluA Man Xyl Fuc Xyl Xyl Xyl
Ver. 1.3SJ 2-3. ph ph ph 1.8 19 ph 7.4 1.2 450 500 400 300 200 100 0 ph 1.8 ph 7.4 3. 1. () 461 476 24 393 ph 1.8pH 7.4 0.2 g 30 37 2 No. 2 10 5
Ver. 1.3SJ 2-4. SOD 4 Fenton 5 30 20 10 SODNU/L mmol /L 100 80 60 40 20 0% 0. 0 5% 0. 10 % 0. 1 5% cont Cont. 4. SOD 0 0% 0.1% 0.5% 1.0 % Cont. 5. 6
Ver. 1.3SJ 3. 3-1. in vitro Kiho 1) Tremella sp.tptp G-6-P TP 2. TP Group Dose (mg/kg) Enzyme activity (mean ± S.E, n=5) 0 3 6(h) Glucokinase N: Control 10.8 ± 1.8 9.1 ± 1.9 11.2 ± 2.1 N: TP 50 14.1 ± 2.6* 15.4 ± 2.0 D: Control 12.5 ± 0.8 12.3 ± 1.5 12.4 ± 1.6 D: TP 50 18.0 ± 1.1* 19.5 ± 2.0* Hexokinase N: Control 19.1 ± 3.4 18.4 ± 1.9 20.7 ± 3.7 N: TP 50 29.8 ± 0.6* 28.1 ± 2.0* D: Control 17.7 ± 1.4 15.0 ± 0.7 15.1 ± 1.9 D: TP 50 20.0 ± 1.5* 21.4 ± 1.6* Glucose-6-phosphate (G-6-P) dehydrogenase N: Control 12.7 ± 1.9 13.0 ± 2.4 18.9 ± 3.1 N: TP 50 22.7 ± 3.1* 27.1 ± 5.8 D: Control 15.7 ± 0.9 16.6 ± 0.1 16.0 ± 2.3 D: TP 50 23.6 ± 1.7* 30.4 ± 8.0* A enzyme unit is defined as the amount that catalyzes the formation of each product per min. * Significant difference from the control at p < 0.05, by Student s t-test. * Normal mouse (N) and streptozocin-induced diabetic mouse (D) 1) Kiho, T., Morimoto, H., Kobayashi, T., Usui, S., Ukai, S., Aizawa, K., Inakuma, T. Effect of a polysaccharide (TAP) from the fruiting bodies of Tremella aurantia on glucose metabolism in mouse liver. Biol. Pharm. Bull., 64, 417-419 (2000). 7
Ver. 1.3SJ 3-2. in vivo 1 % 0.5 ml 30 3. Group (g) (mg/g ) Control 21.5 19.0 19.3 15.3 n = 10 25 25 21.5 (g) 20 15 10 5 19.3 mg/g 20 15 10 5 19.0 15.3 0 Control 0 Control 630 730 4 22 g ± 1 12 1 % 0.5 ml1 1 30 3-3. in vivo Kiho 2) Tremella sp. 0.5 g/l 2) Kiho, T., Morimoto, Kochi, M., T., Usui, S., Hirano, K., Aizawa, K., Inakuma, T.,Effect of an acidic polysaccharide (TAP) from Tremella aurantia and Its degredation product (TAP-H). Biol. Pharm. Bull., 24, 1400-1403 (2001). 8
Ver. 1.3SJ 3-4. in vivo -P 50, 200 mg/kg 30 5 ml/kg24 6 8 200 mg/kg 2 4 (mg/dl) 600 500 400 300 200 100 0 * -1.5 1.5-0.0 2.0 4.0 6.0 (hr) Control 50 mg/kg 200 mg/kg 8. n = 6 ± *p < 0.05 * 18 ddy6 30 50, 200 mg/kg 1 5 ml/kg 2,4 6 E- 9
Ver. 1.3SJ 4. 4-1. in vivo 3 4. Group Triglyceride (mg/dl) Control 216.0 ± 12.4 (0.1 %) 169.8 ± 25.4 (0.2 %) 167.1 ± 15.4 Each value represents mean with the S.E. of 5-6 mice. 5 ddy CRF-1, 0.1 % 0.2 % 3 3 4-2. in vivo Kiho 1) Tremella sp.tp TP50 mg/kg 5. TP Group Dose Relative plasma cholesterol level (mean ± S.E.) (n=15) (mg/kg) 0 3 6 (h) Normal mice Control 0 100 93.4 ± 1.8 95.6 ± 2.4 TP 50 100 74.6 ± 4.7* 91.7 ± 2.7 Diabetic mice Control 0 100 94.7 ± 2.8 93.3 ± 4.8 TP 50 100 67.9 ± 7.9* 78.6 ± 2.7* Each plasma cholesterol level at 0 h normal mice (N=15) 124.6 ± 3.9 mg/dl, diabetic mice (n=15) 141.5 ± 8.2 mg/dl was adjusted to 100. * Significant difference from the control at p < 0.05, by Student s t-test. * Normal mouse (N) and streptozocin-induced diabetic mouse (D) 10
Ver. 1.3SJ 4-3. in vivo Kiho 2) Tremella sp.tp 4-2 TP 6. TP Group (n=4-5) Total bile acid (mmol/g feces) Total cholesterol (mg/g feces) Control TP 96 ± 5.6 135 ± 4.3* 4.73 ± 0.14 4.11 ± 0.13* Feces were gathered for one week after 8 weeks of the test. Each value represents the mean ± S.E.M. of 4-5 mice. Significatly different from the control : * p<0.05. 4-4. 6 2755 P50 mg 1 1 3 7 LDL TG 3 HDL 7. LDL mg/dl 131 ± 37.8 123 ± 31.7 HDL mg/dl 61.3 ± 26.9 66.3 ± 28.5 mg/dl 206 ± 36.4 204 ± 27.9 TG mg/dl 121 ± 97.8 82.7 ± 43.3 meq/l 0.52 ± 0.32 0.51 ± 0.22 5 * LDL mg/dl 70~139 HDL mg/dl 40~77 mg/dl 130~219 TG mg/dl 30~149 meq/l 0.13~0.77 11
Ver. 1.3SJ 200 LDL 120 HDL 300 100 mg/dl 150 100 mg/dl 80 60 mg/dl 250 200 40 50 20 150 300 TG) 1.0 0.8 mg/dl 200 100 meq/dl 0.6 0.4 0.2 0 0.0 9. 9 TG 150 mg/dl 2 TG 1 300 mg/dl 150 mg/dl 1 170 mg/dl TG TG TG LDL 110 mg/dl 2 LDL TG LDL 12
Ver. 1.3SJ 5. 5-1. in vitro MRS Lactobacillus bulgaricus 1.25 % control 1.0 OD = 6 10 8 /ml 3 2 1 Control 0 8h 16h 24h 36h 48h 10. OD 5-2. in vitro MRS Bifidobacterium bifidum 1.25 % control 3 1.0 OD = 610 8 /ml OD 2 1 Control 0 8h 16h 24h 36h 48h 11. 13
Ver. 1.3SJ 5-3. 6 -P50 mg 1 1 3 10 2 12 Clostridium (C.) perfringens 4 Bifidobacterium spp.lactobacillus spp. 10 11 CFU/g Escherichia coli10 10 10 11 CFU/g 10.5 Bifidobacterium spp. 6 Lactobacillus spp. 7 log CFU/g 10.0 9.5 9.0 log CFU/g 5 4 3 log CFU/g 6 5 4 3 8.5 2 2 6 C. perfringens 8 Escherichia coli log CFU/g 5 4 3 log CFU/g 7 6 5 4 3 2 2 12. 14
Ver. 1.3SJ 6. 6-1. 100 6-2. 16 3 2 7 0.1 % 6 5 0.2 % 3 2 1 0 0.10% 0.20% 0.30% 12 24 7 13. 15
Ver. 1.3SJ 7. 7-1. in vitro Kim 3) TFPC12h neurite The neurite length is expressed as the mean S.E.M. of the individual cell (n=150) in each group. Separate measures of one-way ANOVA of the swimming time among the groups were followed by Scheffe test. ## p <0.01, ### p <0.001 vs.control (vehicle). 14. PC12h DFDMEM/Hams F12, 1:1PC12 72 37 5 CO 2 3) Kim. J.H., Ha.H.C., Lee.M.S., Kang.J.I., Kim.H.S., Lee.S.Y., Pyun.K.H., Shim.I. Effect of Tremella fuciformis on the neurite outgrowth of PC12h cells and the improvement of memory in rats. Biol. Pharm. Bull., 30, 708-714 (2007). 16
Ver. 1.3SJ 7-2. in vivo Kim 3) Morris TF TF sham The mean values of the four trials per day for 6 d for each of the four groups are shown. Repeated measures of ANOVA for swimming time among the groups were followed by the LSD test. * p <0.05, ** p < 0.01 vs. sham; # p <0.05 vs. control. 15. TF sham2 mg/kgcontrol 100 mg/kg 400 mg/kg 14 TF-100TF-400Morris Water Maze Test 17
Ver. 1.3SJ 8. 8-1. in vivo Kiho 1, 2) Tremella sp.tp 0.05 %10 30 % 30 % 3-1 8-2. 4-4 1660109 mg/dl 2 120 110 mg/dl 100 90 80 70 16 18
Ver. 1.3SJ 9. 9-1. in vivo 8n = 10 Group mm 2 Control 5.41 ± 1.53 3.23 ± 1.49 p < 0.05 9 0.8 % 10 1 2 3.3 ml 24 3.3 ml 1 1.0 ml 1 9-1. 10 1 % 2 200 ml 7 6 5 4 3 2 1 0 1 3 7 7 * 17. 19
Ver. 1.3SJ 10. 10-1. ph -P ph 2479 1211 ph % 150 100 50 ph 2 ph 4 ph 7 ph 9 0 1 ph 10-2. ph ph 24681012 0.5 -P B 200 [1/s] 25 ph 30 ph Pas 150 100 50 Pas 1000 100 10 1 1 0 1 2 4 6 8 10 12 ph 1 2 3 4 5 6 ph ph 20
Ver. 1.3SJ 10-3. -P 25 2 100 88.48 84.09 2 80 (%) 60 40 20 21.96 20.22 0 21
Ver. 1.3SJ 11. 11-1. (LD50) n = 10-P1000 mg/kg14 P LD50 1000 mg/kg 11-2. n = 20-P227 mg/kg 30 11-3. Ames TA98,TA100 -P Ames 19.55000 µg/plate n = 15-P1.25 g/kg 5 30 11-4. 6 -P50 mg 1 1 3 ASTALTLDγGTLDL HDL TIBCUIBC 11-5. -P 447 19 4 19 11280 22
Ver. 1.3SJ 12. P 1.0 g/100 g 1 0.1 g/100 g 5.6 g/100 g 0.0 g/100 g 2 175 kcal/100 g 3 84.9 g/100 g 2300 mg/100 g 5.8 g/100 g 16.25 2100 3 4 9 4 2 19 10 15 133653 13. P 1 -P 50200 mg 14. ) () P *-P 1 % 23
Ver. 1.3SJ 15. -P () 5 kg 16. 17. <> P 24
Ver. 1.3SJ (Tremella fuciformis) 10~30 %80 % 2 2 25
Ver. 1.3SJ 493-8001 TEL(0586)86-5141() FAX(0586)86-6191 URL/http://www.oryza.co.jp/ E-mail: info@oryza.co.jp 101-0041 1-24-10 5F TEL(03)5209-9150 FAX(03)5209-9151 * * * * P25 2007 10 30 2011 9 20 26