Key words: Surfactant, Tween, Legionella

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Transcription:

Key words: Surfactant, Tween, Legionella

Fig. 1 Inhibitory Activity of Various Tween Against Legionella pneumophila Fig. 2 Inhibitory Activity of Various Tween Against Legionella bozemanii

Fig. 3 Inhibitory Activity of Various Tween Against Legionella longbeachae Fig. 4 Inhibitory Activity of Various Tween Against Legionella gormanii

Table 1 Growth of Legionella strains on various concentration of Tween 20, Tween 40, Tween 60 and Tween 80 in the B-CYE Medium Table 2 Growth of environmental isolates on various concentration of Tween 20, Tween 40, Tween 60 and Tween 80 in the B-CYE medium

Table 3 Relationship between Concentration of Tween 40 and lnoculum Size of Bacterium to test Esterase Activity of Legionella Species + : Positive reaction, : Negative reacfion, W : Week positive reaction, NG No growth *after incubate at 35 Ž, for 4 days Table 4 Relationship between Concentration of Tween 60 and Inoculum Size of Bacterium to test Esterase Activities of Legionella Species + : Positive reaction, : Negative reaction, w: Week positive reaction NG : No growth *After incubate at 35 Ž, for 4 days Legionella-like organism (LLO) L. micdadei, L. bozemanii, L.dumoffii L. jordanis L. pneumophila 3 Legionella L. bozemanii82-192

L. pneumophila Legionella pnemophilc L. bozemanii, L. gormanii L. longbeachae

Classification and identification of Mycobacteria. I,. Tests employing Tween 80 as substrate. Legionella species Amer. Rev. resp. Dis., 90: 588-597, 1965. 2) Sierra, G.: A simple method for the detection of lipolytic activity of microorganisms and some observation on the influence of the cotact L. pneumophila between cells and fatty substractes. Antonic van Leeuwenhock, 23: 15, 1957. 3) Morris, G. K., Skaliy, P., patton, G. M. & Feerey, J. C.: Method for isolating Legionnaires' disease bacterium from soil and water samples. "legionnaires" the disease, the bacterium and methodology (Ed. Jones. G. L. & Hebert, G. A.) 2. L. pneumophila U. S. Department of health Education and Welfare Public Health Service, C. D. C. Althanta, Georgia, 1979, p. 86-90. 4) Rowbotham, T. J.: Pleliminary report on the pathogencity of Legionella pneumophila for fresh water and soil amoebae. J. Clin. Pathol., 33: 1179-1183, 1980. 4. Legionella 7) Pine, L., George, J. R., Reeves, M. W. & Harrell, W. K.: Development of a chemically defined liquid mediuim for growth of Legionella 1) Wayne, L. G., Donbek, J. R. & Russel, R.L.: pneumophila. J. Clin. microbiol., 9: 615-626, 1979.

The Influence of Surfactant (Tween) to the Growth and Producing Activity of Esterase of Legionella Species and Legionella-Like Organisms Katsuhiko SAWATARI, Naomi ITOH, Masao NAGASAWA, Hiroko NAKASATO, Hironobu KOGA, Koichi WATANABE, Hikaru TANAKA, Kiyo FUJITA, Yoshiteru SHIGENO, Keizo YAMAGUCHI, Kinichi IZUMIKAWA, Atsushi SAITO & Kohei HARA 2nd Department of Internal Medicine, Nagasaki University School of Medicine Tween 60, one of the surfactants, is commonly used for isolation of Legionella species from environmental samples as pretreating agent of soil. We have studied on the influence of each Tween (Tween 20, Tween 40, Tween 60 and Tween 80) to the growth and esterase activity of Legionella species and Legionella-like organisms. The tested strains suspended in 0.01 mol phosphate buffer saline (PBS, ph 7.2) were exposed to various cocentrations of the Tweens. As the result, Legionella pneumophila (Philadelphia 1) was not influenced at all of the Tweens, but other strains of Legionella species were decreased, in viable cells after 6 hours exposure to almost all kinds of the Tweens. There was closely correlation between the decrease of viable cells and the concentration of the Tweens. Tween 20 showed bactericidal action against L.bozemanii, L. gormanii and L. longbeachae in the concentration of 0.1%. The, inhibitory activity of Tween 60 against the growth of Legionella species on B-CYE agar was less than those of other Tweens tested, but over than 0.5% concentration of this agent inhibited the growth of almost of all strains except L. pneumophila (ATCC33155, ATCC33215 and ATCC33153). From the result, the procedure of the isolation of Legionella species and Legionella-like organisms from soil must be finished within 6 hours exposure of 0.1 to 0.2% of Tween 60.

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