Seminal paper Anhalt JP Pioneering paper Claydon MA Kishnamurthy T Enterobacter B. cereus Routine identification paper Eigner U Seng P Cherkaoui A Saureus S. Saureus* S. Vagococcus Haemophilus Mycobacterium Listeria B. subtilis Streptococcus B. cepacia E. coli F. tularentis Clostridium Helicobacter 1975 1996 1998 2000 2002 2004 2006 2008 Seng P, et al. Clinical Infectious Diseases. 2009
16S rrna
MALDI-TOF MS CHCA AnchorChip target Autoflex II TOF/TOF MS MALDI BioTyper
+ + + + + + / Time-of-Flight Intensity m/z
Escherichia coli Klebsiella pneumoniae Pseudomonas aeruginosa Staphylococcus aureus 2000 4000 6000 8000 10000 12000 14000 m/z
Rank Score NCBI Matched Pattern (Quality) Value Identifier 1 ( +++ ) Escherichia coli MB11464-1_CHB 2 ( ++ ) Escherichia coli ATCC 25922 THL
1. 2. 3.
39 50
µ µ µ µ µ µ α 4 α
No. of isolates with results No. Species MALDI biotyper No. of Matching at the Matching at the isolates species level genus level Nonmatching 1 Actinobacillus actinomycetemcomitans Actinobacillus actinomycetemcomitans 1 1 2 Aggregatibacter aphrophilus Aggregatibacter aphrophilus 1 1 3 Bacteroides fragilis Bacteroides fragilis 1 1 4 Bordetella parapertussis Bordetella bronchiseptica 1 1 5 Bordetella pertussis Bordetella pertussis 1 1 6 Campylobacter jejuni Campylobacter jejuni 1 1 7 Corynebacterium pseudodiphtheriticum Corynebacterium pseudodiphtheriticum 1 1 8 Enterococcus faecalis Enterococcus faecalis 2 2 9 Erysipelothrix rhusiopathiae Erysipelothrix rhusiopathiae 1 1 10 Escherichia coli Escherichia coli 2 2 11 Fusobacterium nucleatum Fusobacterium nucleatum 1 1 12 Haemophilus influenzae Haemophilus influenzae 2 2 13 Klebsiella pneumoniae Klebsiella pneumoniae 2 2 14 Lactobacillus acidophilus Lactobacillus gasseri 1 1 15 Legionella pneumophila Legionella pneumophila 1 1 16 Listeria monocytogenes Listeria monocytogenes 1 1 17 Moraxella(B.)catarrhalis Moraxella(B.)catarrhalis 1 1 18 Pasteurella multocida Pasteurella multocida 1 1 89.8 8 20 Propionibacterium acnes Propionibacterium acnes 1 1 21 Proteus mirabilis Proteus mirabilis 1 1 22 Pseudomonas aeruginosa 98.0 Pseudomonas aeruginosa 2 2 23 Salmonella sp. Salmonella sp. 1 1 24 Serratia marcescens Serratia marcescens 1 1 25 Shigella sp. Escherichia coli 1 1 26 Staphylococcus aureus Staphylococcus aureus 4 4 27 Staphylococcus epidermidis Staphylococcus epidermidis 1 1 28 Staphylococcus haemolyticus Staphylococcus haemolyticus 2 2 29 Staphylococcus hominis Staphylococcus hominis 2 2 30 Streptococcus parasanguinis Streptococcus pneumoniae 1 1 31 Streptococcus pneumoniae Streptococcus pneumoniae 2 2 32 Vibrio alginolyticus Vibrio alginolyticus 1 1 33 Vibrio cholerae non-o1(nag) Vibrio albensis 1 1 34 Vibrio fluvialis Vibrio furnissii 1 1 35 Vibrio mimicus Vibrio mimicus 1 1 36 Vibrio parahaemolyticus Vibrio parahaemolyticus 1 1 37 Vibrio vulnificus Vibrio vulnificus 1 1 38 Yersinia enterocolitica Yersinia enterocolitica 1 1 39 Yersinia pseudotuberculosis Yersinia pseudotuberculosis 1 1 50 44 5 1
1. 2. 3.
Autoflex II TOF/TOF MS MALDI BioTyper
MALDI BioTyper
1. 2. 3.
Escherichia hi coli 1 Score value 2.314 2.422 2.372 2.377 2000 4000 6000 8000 10000 12000 14000 16000 18000 m/z
Pseudomonas aeruginosa 2 Score value 2.428 2.343 2.396 2.346 2000 4000 6000 8000 10000 12000 14000 16000 18000 m/z
1. 2. 3.
Prevotella buccae 2.341 2.352 Clostridium perfringens 2.435 2.411 2000 4000 6000 8000 10000 12000 14000 16000 18000 m/z
1. 2. 3.
MALDI BioTyper
MALDI BioTyper H21
16S RNA
479/514 93.1 501/514 97.4 16S RNA Enterobacter cloacae Stenotrophomonas maltophilia Enterococcus casseliflavus α streptococcosstreptococcus pneumoniae Shigella spp. Escherichia coli Klebsiella pneumoniae Pseudomonas aeruginosa Streptococcus pneumoniae
1. 2. 3.
1. II 3mL 2. 3000rpm, 5 3. 4. DW D.W 5. MALDI TOF MS 80