Wako
() 538-58441 C-B (344320-M) 1, 3-Fucosyltransferase V, Human Recombinant 50 munits 45,600 Gal1, 4 GlcNc 1, 3-FucT Gal1, 4 (Fuc1, 3) GlcNc 075-03891 GDP-Fucose GDP-Fucose GDP 2 mg 50,000 535-58451 C-B (345650-M) 1, 4-Galactosyltransferase, Bovine, Recombinant 1 unit 22,900 GlcNc 1, 4-GaIT Gal1, 4GlcNc GDP-Galactose GDP 512-08453 516-08451 SIG (U4500) UDP-Galactose 5 mg 25 mg 10,000 32,900 532-58461 C-B (444125-M) 1, 2-Mannosyltransferase, Catalytic Domain, Saccharomyces cerevisiae, Recombinant 100 munits 34,200 Man1, 2Man-Thr (Ser) -Protein 1, 2-ManT Man1,2 Man1, 2Man-Thr (Ser) -Protein GDP-Mannose GDP 517-44864 SIG (G5131) 512-45931 SIG (G7377) GDP-Mannose (Yeast) 50 mg 32,800 GDP-Mannose (Synthetic) 5 mg 7,600 530-58261 C-B (566218-M) 2, 3- (N) -Sialyltransferase, Rat, Recombinant 100 munits 45,600 Gal1, 3 (4) GlcNc 2, 3-ST Neuc2,3Gal1,3 (4) GlcNc CMP-Neuc CMP 030-13531 CMP-Neuc 10 mg 16,800 537-58271 C-B 2, 6-Sialyltransferase, Rat Liver 100 munits 28,600 (566217-M) 196-10581 2, 6-Sialyltransferase, Rat Liver 50l 17,000 Gal1, 4 GlcNc 2, 6-ST Neuc2,6Gal1,4GlcNc CMP-Neuc CMP 030-13531 CMP-Neuc 10 mg 16,800
N- N 016-14991 020-12631 N 032-14691 043-24931 040-24941 047-24951 135-12071 N 536-37621 TOR (B6825) 30,000 10mg 10mg 10mg 10mg 10mg 10mg 10mg 25mg 30,000 10,000 20,000 25,000 30,000 30,000 18,200 198-10281 1mg 19,000 022-11111 2mg 15,000 028-11113 10mg 38,000 539-37591 TOR 100mg 23,100 (C6660) C-B 25mg (252927-M) 533-37631 TOR 10mg 34,500 (D2360) C-B 5mg (259544-M) 202-08241 10mg 16,500 N 208-08243 50mg 50,000 N
1) Suzuki, S. S. et al. : J. Cell. Biochem., 51, 181 (1993) 2) Seftor, R. E. B. et al. : Melanoma Res., 1, 43 (1991) 3) J. Biol. Chem., 261, 4766 (1986) 4) Eur. J. Biochem., 150, 41 (1985) 1) Myers, R. W. et al. : nal. Biochem., 101, 166 (1980) 2) Potier, M. et al. : nal. Biochem., 94, 287 (1979) 214-00941 210-00943 215-00971 515-71171 511-71173 518-71203 211-00951 217-00953 SIG (U4375) SIG (U5252) UDP-Glucose2Na UDP-N-cetyl Glucosamine2Na UDP-N-cetylglucosamineNa UDP-N-cetylgalactosamine2Na UDP-Glucuronic cid3na 250 mg 1 g 100 mg 25 mg 100 mg 5 mg 100 mg 500 mg 5,000 17,000 9,000 4,900 12,700 14,100 7,000 28,000
l l l l l l l l l l l
PI PIP2 PLCIP3DG CaCaMKPKC PIIP3 Ca 2 IP3C PBS (0.5mgml) No.IPR.1 IgG2b IP3C 150015,000 1 201 50 1 5011,000 l PI3p85 No.U5 IgG1 PI3p85 1200 () l PI3p85 No.T4 IgG1 PI3p85 11,000 () llllllllllllllllllllllllllllllllllll
80 () --D- (-) HS-J - 0.0080.03EUml 1. LL HS-T 0.2ml25 2. Control Standard Endotoxin 500ng (LPS) 40 20 r =0.996 10 0.002 0.004 0.008 0.016 0.032 0.064 0.128 0.256 l (0.0150.03EUml) (0.03EUml) sssssssssssssssssssssssssssssssssssss LED () () SLP 3037 291-25751 299-28351 297-28651 291-28051 1,900,000 1,800,000 200,000 200,000
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l (Cryptosporidium parvum, C. muris, C, baileyi) ( ) // () ml (Cryptosporidium parvum) ()FITC 2.7ml ( 10) () 50ml ()10 100ml
() () (IL-1, IL-4, IL-6, IL-10) () () () HRP (100) l l 30ml 7ml12ml 0.15ml () HRP 15ml () (TMBz) 12ml () (25) 50ml () 12ml (10) l l l IL-1IL-1IL-1IL-3IL-5IL-6IL-7 IL-8IL-10IL-12IL-13GM-CSFIFN-IL-2IL-6IL-10IFN-TNF-TNF- MIP-2MCP-1 l l l IL-4IL-1IL-2IL-3IL-4IL-5IL-7IL-8IL-10IL-12IL-15IL- 2IL-3IL-4IL-6IFN-TNF-MCP-1MIP-2TNF-
l l l IL-6IL-1IL-2IL-6IL-7IL-8IL-13IL-15IFN- IL-1IL-2IL-10MCP-1TNF-IL-1IL-4IL-10TNF-MIP-2 l l l IL-10IL-1IL-7IL-8IL-10IL-15IFN-GM-CSFRNTESTNF- IL-1IL-2IL-3IL-4IL-6IL-10TNF-IL-1IL-4TNF-IFN-MIP-2 Wako Wako Wako
536-54961 0238 Group 531-55011 0329 GroupGroup 530-54981 0320 GroupGroup 537-54991 0323 Group 534-55001 0326 GroupmGluR2mGluR4 100 mg 6,500 5 mg 26,500 5 mg 26,500 5 mg 19,500 5 mg 19,500 539-55051 0339 5 mg 54,000 538-55021 0330 5 mg 91,000 535-55031 0331 5 mg 54,000 533-54971 0285 NMD 5 mg 40,000 NMD 532-55041 0332 5 mg 54,000 TOCRIS COOKSON 1997FX WKO BIO WINDOWFX06-201-5965
(PTN) (MK) 1) PTN136 MK 2) 50% PTN 1) 1) 3) 4) l PTNMK MK PTNPTNMK PTNPTNMK PTNMK 1) Science, 250, 1690-1694 (1990) 2) J. Pept. Sci., 2, 28-39 (1996) 3) Biochem. Biophys. Res. Commun., 173, 246-251 (1990) 4) Proc. Natl. cad. Sci. US, 93, 14753-14758 (1996) 334-43351 337-42981 334-42991 4335-v 4298-v 4299-s Pleiotrophin (Human) Midkine (Human) Midkine (Human, 60-121) 50g 50g 0.1 mg 30,000 30,000 30,000
l l l l l l l 535-60041 532-60051 539-60061 536-60071 533-60081 530-60091 533-60101 530-60111 537-60121 534-60131 531-60141 538-60151 535-60161 532-60171 539-60181 536-60191 539-59951 536-59961 539-60201 536-60211 533-60221 BK021 BK022 BK001 BK002 BK003 BK004 BK005R BK005F BK006 BK007F BK007R BK008 BK009 BK010 BK012 BK014 BK015 BK016 BK017 BK018 BK020 (Muscle ctin) (Non-muscle ctin) (Rhodamine based Kit) (Fluorescein based Kit) (Fluorescein based Kit) (Rhodamine based Kit)
l l l l l l l l l l l l () 95 V V 80 13 2-1 4.25.2 V V ccccccccccccc 80 (1-2-) 14 (2-) 4.25.2 V V 206-14077 209-14067 95 V 95 V 18l 18l 9,000 9,800 205-13741 203-13747 202-13871 200-13877 9l 18l 9l 18l 5,500 9,800 6,900 10,500
M (EthD-1) M (em530nm) EthD-1 (em>600nm) 3045 51 Cr M40l [4mM DMSO] 150l [2mM DMSO/H2O 1 : 4 (v/v )] 1,000 1,000 300 SYTO10 (em510nm) DED Red (em600nm) 24 SYTO1050l [DMSO]1 DED Red50l [DMSO (20%) ]1 100 100
NKLKT DiOC18 (ex : 484nm,em : 501nm) Propidium iodide (ex : 536nm,em : 617nm) 51 Cr DiOC18200l [DMSO]1Propidium iodide1.1ml [3.75mM ]1 2,000 200 () (10l) () 0.15610.0ngml 1. 961 2. 200ngml 20l 3. 60ml 4. 5l 5. - 10l 6. OPD 2 7. 12ml 8. (1moll H2SO4) 12ml 9. (10) 50ml
ISOHIR () DN 1DN DN 1cm6cmDN 1/4p53 (exon11)seminested PCR (first PCR1296bpsecond PCR 265bp) MCT118116 VNTR (variable number of tandem repeat) BC 36cm DN1/4 MCT118 PCR DN 15cm PCR DN T4 gene 32 proteinpcr PCR (lane 1)DN () DN1/20 (lane 2)T4 gene 32 protein (lane 3)DN DN (280bp) PCR (lane 4) T4 gene 32 protein (lane 5) T4 gene 32 proteint4 gene 32 proteinpcr T4 gene 32 protein
1mm 0.5mg1mg 2.5mg5mgDN1/10 DN (D 279bpp53 (exon10 279bp) PCR p53 TPduplex DN duplex DN, blant duplex DN, nicked duplex DNRN-DN hybrid partial DN 620,000unitsmg (TPase ) 97 (SDS-PGE) 200lml 76kDa uvrde. coli 20l371nmoleTP 1unit40ng helicase92bpduplex DN50 20mM Tris-HCl (ph8.3) 200mM NaCl25mM 2- mercaptoethanol1mm EDT0.5mM EGT 50 Glycerol 80 20l25mM Tris-HCl (ph7.5) 20mM NaCl3mM MgCl22mM 2-mercaptoethanolmM TP2M 92bp partial duplex DN helicase3710 J. Biol. Chem., 262, 2066 (1987) 100 75 50 25 0 20 40 M 60 80 1) Matson, S. W. and George, J. W. : J. Biol. Chem., 262, 2066 (1987) 2) Runyon, G. T. and Lohman, T. M. : J. Biol. Chem., 264, 17502 (1987) 3) Runyon, G. T. and Bear, D. G. et al. : Proc. Natl. cad. Sci. US, 4) Matson, S. W. : Proc. Natl. cad. US, 86, 4430 (1989) 5) Geoprge, J. W., Brosh, R. M. et al. : J. Mol. Biol., 235, 424 (1994) 6) Rumyon, G. T., Wong, I. and Lohman, T. M. : Biochemistry, 32, 602 (1993) 87, 6383 (1990)
1) (P ) 2) 3) 10 4 P DN (a) (b)dndn (P) (c)p PP DNDN (d) DN (e) RPP (f)pod (g)p
ELIS 1 ) (DN 96 12) 1 2 ) 2 3 ) 100ml 4 ) 3 12ml 5 )HRP (400) 0.1ml 6 ) 12ml 7 ) 12ml 8 ) (20) 100ml 9 ) (2.5N) 12ml 10) 4 11) DN 3 (H3) - -HRPHRP ttttttttttttttttttttttttttttttttttttttttttttttt IL-3TF-1 IL-372 DN bcl-2elis (I25) bcl-2 ELIS (I23) DN IL-324 DN (1) bcl-2 ( 2) 539-48081 I25 Nucleosome ELIS 96 45,600
B D E C G H F H FXEmail a,b,c,d,,,,fx 10 (3,000) a b c d 1031 FX06-201-5965 EmailXLM04256niftyserve.or.jp No.7 "" 18410 5413-1-2 () 11 13Wako @1 @2 sssssssssssssssssssssssssssssssssssssssssssssssssss
WKO BIO WINDOW No.8 OCT. 1997 MediaKapMediaKap PlusPlus 534-58281 ME2M02018S 0.2m MediaKap 2 0.22l 18 17,500 531-58291 ME2M05018S 0.2m MediaKap 5 25l 18 36,500 534-58301 ME2M10018S 0.2m MediaKap 10 510l 1/4 () 18 56,000 531-58311 ME2M02B12S 0.2m MediaKap 2 0.22l 12 14,000 538-58321 ME2M05B12S 0.2m MediaKap 5 25l 12 27,000 535-58331 ME2M10B12S 0.2m MediaKap 10 510l 1/4 () 12 40,000 532-58341 ME2M25B06S 0.2m MediaKap 25 1025l 1/4 () 6 31,000 539-58351 ME2M50B03S 0.2m MediaKap 50 2550l 1/4 () 3 22,500 536-58361 MP2M02018S 0.2m MediaKap 2 Plus 0.22l 18 28,500 533-58371 MP2M05018S 0.2m MediaKap 5 Plus 25l 18 45,500 530-58381 MP2M02B12S 0.2m MediaKap 2 Plus 0.22l 12 20,500 537-58391 MP2M05B12S 0.2m MediaKap 5 Plus 25l 12 33,500 530-58401 MP2M10B12S 0.2m MediaKap 10 Plus 510l 1/4 () 12 50,500 537-58411 MP2M25B06S 0.2m MediaKap 25 Plus 1025l 1/4 () 6 38,000 534-58421 MP2M50B03S 0.2m MediaKap 50 Plus 2550l 1/4 () 3 26,000