sher.com/separate
|
|
|
- しげのぶ つつの
- 5 years ago
- Views:
Transcription
1
2 sher.com/separate
3 , 69, 71, Ordering information 83
4 4 ph 2 PAGE N,N,-methylenebisacrylamide bis PAGE Mini Gel Tank
5 5 vs. vs. 1 2 Tris-Glycine vs. 2 : SDS SDS 11.4 g SDSSDS- / DTT- -ME tris 2-carboxyethyl phosphine TCEP 1 1 2? Arne Tiselius Ornstein L (1964) Disc electrophoresis. 1. Background and theory. Ann N Y Acad Sci 121:
6 6 SDS-PAGE Invitrogen NuPAGE System Bis-Tris Tris-acetate Laemmli Trisglycine NuPAGE System ph Tris-glycine1 3 : Tris+ Tris-Glycine ph 9.5 Tris-acetate33 : Tricine Tris + ph Tris-Glycine 1 23 Tris 1. Tris-glycine Tris ph 8.7 Tris, glycine,sds ph 8.3 ph 9.5 Bis-Tris23 : MES MOPS MES: 2- N- MOPS: 3- N- Bis-Tris + Tris + phph 6.4pH ph ph7.0 Bis-tris Tris 2. Bis-Tris Bis-Tris ph 6.4 Tris, MES MOPS SDS ph 7.3 ph The Tris-acetate Tris ph 7.0 Tris tricine SDS ph 8.3 ph 8.1
7 7 1 sher.com/proteingels Bolt Bis-Tris Plus NuPAGE Bis-Tris gel NuPAGE Tris-Acetate gel Bolt Bis-Tris Plus gel Novex Tris-Glycine gel Novex Tricine gel NativePAGE gel Novex IEF gel Novex Zymogram gel E-PAGE gel? 45 Ulrich K. Laemmli T4 SDS- PAGE sher.com/gelcastingaccessories
8 8 (>2.5 kda) (<500 kda) Novex Tricine gel NuPAGE Tris-Acetate gel E-PAGE Coomassie () NuPAGE Bis-Tris gel NuPAGE Bis-Tris gel NuPAGE Bis-Tris gel Bolt Bis-Tris Plus gel Bolt Bis-Tris Plus gel Bolt Bis-Tris Plus gel Bolt Bis-Tris Plus gel Novex WedgeWell Tris-Glycine gel 1 NativePAGE gel Novex Tris-Glycine gel Novex IEF gel ZOOM IPG Novex Zymogram gel 2 NuPAGE Bis-Tris gel 2D NuPAGE Bis-Tris gel 2D NuPAGE Bis-Tris ZOOM gel IPG sher.com/minigelselection 83-91
9 9 Bis-Trisvs. Tris-glycine A B SDS-PAGE Laemmli Tris-glycine ph Tris, ph 8.3 Tris, ph 6.8 Tris, ph 8.8Laemmli ph Laemmli : ph 8 ph LaemmlipH C Asp-Pro 4. A Bolt Bis-Tris PlusB Tris-glycine Tris-glycine NuPAGEBolt Bis-Tris HCIpH 6.4 ph7.0 Bis-TrispHLaemmli : 8 12 ph Moos et al C 10 Asp-Pro Invitrogen NuPAGE Antioxidant 70% , 5 1 2D/IPG mm1.5 mm >30 μl1.5 mm 5 Bolt 1.5mm1.0mm
10 10 SureCast Gel Handcast System 100% leak-free Invitrogen SureCast Gel Handcast System SureCast Gel Handcast System 100% (load-and-lock) SureCast Gel Handcast System Invitorogen SureCast Gel Handcast Reagent For ordering information refer to page XX. For quick reference on the protocol please refer to page XX. 83
11 11 SureCast Gel Handcast Reagents SureCast Stacking Buffer and Resolving Buffer Invitrogen SureCast Stacking BufferResolving Buffer 500 ml 0.5 M ph ml 1.5 M ph 8.8 SureCast Gel Handcast 1 ph SureCast Gel Handcast System SureCast Gel Handcast Invitrogen SureCast Acrylamide Solution (40%) SureCast Handcast System 40
12 12 Bolt Bis-Tris Plus mini gel ph Invitrogen Bolt Bis-Tris Plus gel 67 ph 52 Bolt Bis-Tris Plus gel: ph Invitrogen Novex Bis-Tris Plus chemistry 10% Rf CV 2% 5. Bolt Bis-Tris Plus : 16 カ月 : 35 : kda : 8%, 10%, 12%; エ 4 12% : 8 x 8 cm 1 mm 12 : 40 μl, ル 2/3 Mini Gel 6. Bolt Bis-Tris Plus Bolt 4 12% Bis-Tris Plus 10 μl Mini Gel Tank 200 V Invitrogen SimplyBlue SafeStain 1: Invitrogen SeeBlue Plus2 Prestained Standard 2: 10 μg E. coli3: Invitrogen Mark12 Unstained Standard 12 4: 40 μg HeLa5: 20 μg HeLa 6: 5 μg BSA 7: 40 μg Jurkat 8: 5 μg GST 9: Invitrogen Novex Sharp Unstained Protein Standard 10: 5 μg -galactosidase sher.com/bolt 8378
13 13 Bolt Bis-Tris Plus 7. Bolt Bis-Tris Plus Bolt system 23 Bolt Crystal M. 1 Trisglycine Bolt Bis-Tris Susan S.? Timothy Updyke Sheldon Engelhorn ph Bis-Tris 1996 Invitrogen Bolt Welcome Pack + iblot 2 System Mini Gel Tank Invitrogen Bolt SeeBlue Plus2 Prestained Standard Invitrogen iblot 2 Gel Transfer Device Bolt Bis-Tris Plus gel Coomassie Thermo Scientific Pierce Power Stainer Bolt Welcome Pack + iblot 2 System
14 14 NuPAGE gel 20,000 Invitrogen NuPAGE SDS-PAGE gel system Laemmli NuPAGE gel ph 2 Invitrogen NuPAGE Bis-Tris gel Invitrogen NuPAGE Tris-Acetate 89 NuPAGE: ph ph : NuPAGE Bis-Tris gel: 16 NuPAGE Tris-Acetate gel: 8 : 35 : NuPAGE Bis-Tris gel: kda NuPAGE Tris-Acetate gel: kda : NuPAGE Bis-Tris gel: 8%, 10%, 12%; 4 12% NuPAGE Tris-Acetate gel: 7%; 3 8% : : 8 x 8 cm mm : 8 x 13 cm 1 mm 10 : 25 μl 1 mm ; 37 μl 1.5 mm A. B. 8. NuPAGE Bis-TrisTris-Acetate 10 μl A Invitrogen NuPAGE 4 12% Bis-Tris B Invitrogen NuPAGE 3 8% Tris-Acetate Mini Gel Tank200 V SimplyBlue SafeStain A B 1: SeeBlue Plus2 Prestained Standard; 2: 10 μg E. coli; 3: Mark12 Unstained Standard 12 ; 4: 40 μg HeLa; 5: 20 μg HeLa; 6: AB 5 μg BSA; 7: 40 μg Jurkat; 8: 5 μg GST ; 9: Novex Sharp Unstained Protein Standard; 10: 5 μg -galactosidase Mini Gel sher.com/nupage
15 15 NuPAGE gel A. NuPAGE Bis-Tris gel B. NuPAGE Tris-Acetate gel C. NuPAGE Tris-Acetate gel 9. NuPAGE gel A NuPAGE Bis-TrisInvitrogen Novex Sharp Prestained Protein StandardNovex Sharp Unstained Protein Standard B NuPAGE Tris-Acetate gel HiMark Unstained Protein Standard C Tris-acetate Invitrogen NativeMark Unstained Protein Standard Invitrogen HiMark UnstainedPrestained Protein Standard NuPAGE Tris-Acetate ready-to-use kda 9 PageRuler PageRuler PlusSpectra Prestained Protein Ladder NuPAGE Bis-Tris gel Coomassie 64
16 16 Novex WedgeWell Tris-Glycine gels 60 μl Invitrogen Novex WedgeWell Tris-Glycine gels Laemmli PAGE Novex WedgeWell Tris-Glycine gels 60 μl Mini Gel 10 WedgeWell Tris-Glycine gels A20 μl 60 μlnovex WedgeWell Tris-Glycine 10-well gel B20 μl 60 μl BB 50 μl 60 μl
17 17 WedgeWell Tris-Glycine gels WedgeWell Tris-Glycine gels Large proteins ( kda) Mid-size proteins ( kda) Small proteins (3 60 kda) Wide range (6 200 kda) 6% 8% 10% 12% 14% 16% 4 12% 8 16% 4 20% 10 20% kda kda 200 kda 200 kda 200 kda 116 kda 116 kda 97 kda 97 kda 200 kda 116 kda 97 kda 66 kda 200 kda 200 kda 116 kda 97 kda 66 kda Percent length of gel kda 116 kda 97 kda 116 kda 97 kda 66 kda 55 kda 66 kda 55 kda 36 kda 66 kda 55 kda 36 kda 31 kda 55 kda 36 kda 31 kda 200 kda 116 kda 97 kda 116 kda 97 kda 66 kda 55 kda 116 kda 97 kda 66 kda 55 kda 55 kda 36 kda 31 kda 66 kda 21 kda 66 kda 36 kda kda 97 kda 55 kda 36 kda 31 kda 21 kda 14 kda 55 kda 36 kda 31 kda 31 kda 21 kda 21 kda 14 kda 31 kda 21 kda 14 kda 6 kda 36 kda 21 kda 14 kda 6 kda kda 6 kda 31 kda 14 kda 6 kda 6 kda kda 11 Novex WedgeWell Tris-Glycine gels Invitrogen Novex Mark12 21 kda Pierce SDS-PAGE Sample Prep Kit. Invitrogen Novex Tris-Glycine SDS Sample Buffer and Tris-Glycine SDS Running Buffer. Invitrogen Novex Tris-Glycine Native Sample Buffer and Tris-Glycine Native Running Buffer. PageRuler, PageRuler Plus, and Spectra protein ladders Novex Tris-Glycine gels
18 18 NativePAGE gel Invitrogen NativePAGE Bis-Tris gel system BN PAGE Coomassie G NativePAGE systemph NativePAGE gel System : 15 kda10 MDa 12 ph Tris-glycine Tris-glycine gel systemnativepage gel System : : 6 : 90 : 15 10,000 kda : 3 12% 4 16% : 8 x 8 cm 1 mm 10 : 25 μl Is there a higher res pic somewhere? I copied and pasted this from source file. Mini Gel 12. NativePAGE 2Mini Gel Invitrogen NativePAGE Novex 3 12% Bis-Tris Protein Coomassie 1 10: ; 2 6: 5 μl NativeMark Unstained Protein Standard; 3 4 5: μg ; 7 8 9: μg Coomassie G-250 Coomassie G sher.com/nativepage 8880
19 19 NativePAGE gel? 1991 Hermann Schagger Gebhard von Jagow 13. NativePAGE gel NativeMark Unstained Protein Standard NativePAGE gel NativeMark Unstained Protein StandardTris-glycine gel Tris-acetate gel NativePAGE gel system 20 1,200 kda 242 kda- 42
20 20 Novex Tricine gel Invitrogen Novex Tricine gel systemtris-glycine System 14 Tris-glycine Novex Tricine gel : 2kDa 15 : 1 2 : 90 : 2 20 kda : 10% 16%; 10 20% : 8 x 8 cm 1 mm 10 : 25 μl PVDF ph Mini Gel Novex Tricine gel Tris-glycine DS <10 kdads DS Novex TricinepH Tris-glycineDS Novex Tricine gel System 14. Novex Tricine 10 μl Invitrogen Novex 10 20% Tricine Mini Gel200 V SimplyBlue SafeStain 1: SeeBlue Plus2 Prestained Standard; 2: 10 μg E. coli; 3: Mark12 Unstained Standard 12 ; 4: 40 μg HeLa; 5: 20 μg Hela; 6: 5 μg BSA; 7: 40 μg Jurkat ; 8: 5 μg GST; 9: Novex Sharp Unstained Protein Standard; 10: 5 μg -galactosidase sher.com/tricine 8881
21 21 Novex Tricine gel 15. Novex Tricine gel? ph Novex Tricine gel In-Gel Tryptic Digestion Kit
22 22 Novex IEF gel IEF pi1 pi phinvitrogen Novex IEF gel ph pi16 17pI SDS-PAGE : 2 : 2.5 : ph 3 10 : pi ph 3 7 : pi : 5% : 8 x 8 cm 1 mm 10 : 20 μl Novex IEF gel : pi pl Mini Gel SDS- PAGE Novex IEF gelmini 6.0 Gel IEF Marker IEF Marker 3 10 pi = 7.8, 8.0, 8.3pI = pi = 6.0- pi = pI = 4.5 pi = 4.2 Coomassie R-250 IEF 3-10 : 1 6: 20 μl; 2 7: 10 μl; 3 8: 5 μl; 4 9: 2.5 μl; 5 10: sher.com/ief 8881
23 23 Novex IEF gel? ph Harry Svensson-Rilbe Olof Vesterberg Novex IEF marker Novix IEF gel 1: Aspergillus niger pi = 3.5; 2: Aspergillus nigerpi = 4.2; 3: pi = 4.5; 4a 4c: - pi = : pi = 6.0; 6a 6c: pi = ; 7a 7m 7c: Lens culinarispi = ; 8: A pi = 9.5; 9: cpi = Novex IEF buffer kits IEF Marker 3 10 Invitrogen ZOOM IEF Fractionator Combo Kit
24 24 Novex Zymogram gel 1. Novex Zymogram Novex Zymogram gelatin gel Novex Zymogram casein gel Novex Zymogram blue casein gel Invitrogen Novex Zymogram gel Novex Zymogram gel % Tris- Glycine 12% Tris- Glycine 4 16% Tris- Glycine 0.1% 0.05% 0.1% 10 6 unit 7 x 10 4 unit 1.5 x 10 3 unit Yes Yes No? kda kda kda Novex Zymogram gel? SDS Novex Tris-Glycine SDS Novex Zymogram gel Invitrogen Novex Zymogram renaturing buffer Invitrogen Novex Zymogram developing buffer : 2 : 90 : kda 19 : 10% 12% ; 4 16% : 8 x 8 cm 1 mm : 20 μl Novex ZymogramMini GelI Invitrogen Novex 10% Zymogram Novex Zymogram renaturing buffernovex Zymogram developing buffersimplyblue SafeStain 3 7: 5 μl of 2.0 μu/ml I ; : 12 μl SeeBlue Prestained Protein Standard Mini Gel sher.com/zymogram 8882
25 25 Novex Zymogram gel 10% 12% gel 4 16% gel % Novex Zymogram gel : 1: Type I 140 kda 2: 37 kda 3: 30 kda 4: 19 kda Zymogram renaturing buffer Zymogram developing buffer
26 26 E-PAGE High-Throughput Precast Gel System Invitrogen E-PAGE High-Throughput Precast Gel System Invitrogen E-PAGE 48-well96-well precast gel UV E-PAGE System E-Base E-Holder E-Editor 2.0 Software E-PAGE High-Throughput Precast Gel System : 23 : 6 : 14 : kda : E-PAGE 48 gel: 8% E-PAGE 96 gel: 6% : 13.5 x 10.8 cm 3.7 mm : E-PAGE 48 gel: 20 μl E-PAGE 96 gel: fi xed 6% A B E-PAGE? E-PAGEInvitrogen E-Base electrophoresis device E-PAGE gelinvitrogen Mother E-Base devices Mother E-Base devices 2 Invitrogen Daughter E-Base devices Mother E-Base Daughter E-Base C E-PAGE 96 gel 20. E-PAGE gela E-PAGE 48 gel B E-PAGE 96 gel C Mother E-Base device Daughter device sher.com/epage 88
27 27 E-PAGE gel 0% E-PAGE 48 8% Gel E-PAGE 96 6% Gel 0% 10% 20% 30% 40% 50% 220 kda 120 kda 100 kda 80 kda 60 kda 50 kda 220 kda 120 kda 60 kda 40 kda 20 kda 25% 50% 75% 100% 21. E-PAGEInvitrogen E-PAGE MagicMark Unstained Protein Standard? E-Base device The Society for Biomolecular Screening SBS 40 kda 60% 30 kda 70% 20 kda 80% 90% 100% E-PAGE SeeBlue Prestained Protein Standard E-PAGE MagicMark Unstained Protein Standard E-PAGE
28 28 : 1X 29 : :SDS100ºC Kubo, ºC 2 5 Native Novex Zymogram Gels : : SDS : DNA DNA 2 RIPA Triton X kda SDS-PAGE Thermo Scientifi c Pierce SDS-PAGE Sample Prep Kit 88-89
29 29 Pierce SDS-PAGE Sample Prep Kit M S M S Pierce SDS-PAGE M S M S SDS-PAGE Thermo Scientifi c Pierce SDS-PAGE Sample Prep Kit 10 Pierce SDS-PAGE Sample Prep Kit : SDS-PAGE 22 Thermo Scientific Pierce BCA Assay SDS-PAGE μg 22. C6 Thermo Scientific MemPER Eukaryotic Membrane Protein Extraction Reagent Kit Pierce SDS-PAGE Sample Prep Kit 4 20% SDS-PAGE 4 COX4 Thermo Scientific SuperSignal West Femto chemiluminescent substrate Kit S = M = % % 75% 85% 77% 77% c 23. Pierce SDS-PAGE Sample Prep Kit kda60 μg Pierce BCA Protein Assay Kit 74% How does it work? Pierce SDS-PAGE Sample Prep Kit DMSO 70 μg μl 20 μl Pierce SDS Protein Binding Resin DMSO 50 μl sher.com/pagesampleprep μg BSA) 75% 0.5 M 80% 2 M 76% 20% SDS 75% 10% Triton 75% 6 M :DMSO 1:3 75% 1 M 75% 6 M 74% 10% CHAPS 80% 25% 71% 10% OTG 71% 2 M 70% 40% 70%
30 30 PAGE 2-10 SDS-PAGE sher.com/electrophoresisbuffers 88-89
31 31 SDS-PAGE Bolt Bis-Tris Plus gel NuPAGE Bis-Tris gel NuPAGE Tris-Acetate gel Novex WedgeWell Tris-Glycine gel Novex Tricine gel NativePAGE gel Novex IEF gel Bolt Sample Reducing Agent 10X Bolt LDS Sample Buffer 4X Bolt Antioxidant NuPAGE Sample Reducing Agent 10X NuPAGE Antioxidant NuPAGE LDS Sample Buffer 4X Novex Tris-Glycine SDS Sample Buffer 2X NuPAGE Sample Reducing Agent 10X Novex Tris-Glycine Native Sample Buffer 2X Novex Tris-Glycine SDS Sample Buffer 2X NuPAGE Sample Reducing Agent Novex Tris-Glycine Native Sample Buffer 2X Novex Tricine SDS Sample Buffer 2X NativePAGE Sample Buffer 4X NativePAGE 5% G-250 Sample Additive Novex IEF Sample Buffer ph X Novex IEF Sample Buffer ph 3 7 2X Pierce LDS Sample Buffer 4X Pierce Lane Marker Non- Reducing Sample Buffer 5X Pierce Lane Marker Non- Reducing Sample Buffer 5X Bolt MES SDS Running Buffer 20X Bolt MOPS SDS Running Buffer 20X NuPAGE MES SDS Running Buffer 20X NuPAGE MOPS SDS Running Buffer 20X NuPAGE Tris-Acetate SDS Running Buffer 20X Novex Tris-Glycine NativeRunning Buffer 10X Novex Tris-Glycine SDS Running Buffer 10X Novex Tris-Glycine Native Running Buffer 10X Pierce Tris-Glycine SDS Buffer 10X BupH Tris-Glycine-SDS Buffer Pack Novex Tricine SDS Running Buffer 10X NativePAGE Running Buffer 20X NativePAGE Cathode Buffer Additive 20X Novex IEF Anode Buffer 50X Novex IEF Cathode Buffer ph X Novex IEF Cathode Buffer ph X MES vs. MOPS : MES SDS MOPS MES MOPSpKa MES MOPS SDS 2 : : Bolts Sample Reducing Agent NuPAGE Sample Reducing Agent DTT 50 mm - - ME2.5% tris 2-carboxyethyl phosphine TCEP 50 mm 1 Novex Zymogram gel* Novex Tris-Glycine SDS Sample Buffer 2X Novex Tris-Glycine SDS Running Buffer 10X * Novex Zymogram gelnovex Zymogram developing buffer 10X Novex Zymogram renaturing buffer 10X
32 32 NuPAGE buffer 1X NuPAGE LDS Sample Buffer C Glycerol Tris base Tris HCl LDS EDTA SERVA Blue G-250 Phenol Red 0% 141 mm 106 mm 2% 0.51 mm 0.22 mm mm (ph 8.5) NuPAGE MOPS SDS Running Buffer* C MOPS Tris base SDS EDTA 50 mm 50 mm 0.1% 1 mm (ph 7.7) NuPAGE MES SDS Running Buffer* C MES Tris base SDS EDTA 50 mm 50 mm 0.1% 1 mm (ph 7.3) NuPAGE Transfer Buffer C Bicine Bis-Tris (free base) EDTA Chlorobutanol 25 mm 25 mm 1.0 mm 0.05 mm (ph 7.2) NuPAGE Tris-Acetate SDS Running Buffer C Tris base Tricine SDS 50 mm 50 mm 0.1% (ph 8.24) * NP0001NP0002NuPAGE AntioxidantNP
33 33 Tris-glycine buffer 1X Tris-Glycine SDS Sample Buffer +4 C Tris HCl* Glycerol SDS Bromophenol Blue Deionized water 63 mm 10% 2% % (ph 6.8) Tris-Glycine Native Sample Buffer +4 C Tris HCl* Glycerol Bromophenol Blue Deionized water 100 mm 10% % (ph 8.6) Tris-Glycine SDS Running Buffer Room temperature Tris base Glycine SDS Deionized water 25 mm 192 mm 0.1% (ph 8.3) Tris-Glycine Native Running Buffer Room temperature Tris base Glycine Deionized water 25 mm 192 mm (ph 8.3) Tris-Glycine Transfer Buffer Room temperature Tris base Glycine Deionized water 12 mm 96 mm (ph 8.3) * Tris HClTris6 N HCl ph
34 34 Tricine buffer 1X Tricine SDS Sample Buffer +4 C Tris HCl* Glycerol SDS Coomassie Blue G Phenol Red Deionized water 450 mm 12% 4% % % (ph 8.45) Tricine SDS Running Buffer Room temperature Tris base Tricine SDS Deionized water 100 mm 100 mm 0.1% (ph 8.3) * Tris HClTris6 N HCl ph Zymogram buffer 1X Zymogram Renaturing Buffer Room temperature Triton X-100 Deionized water 2.7% (w/v) in H 2 O Zymogram Developing Buffer Room temperature Tris HCI* NaCl CaCl 2 2 H 2 O Brij 35 Deionized water 50 mm 200 mm 5 mm 0.006% (w/v) _ (ph 7.6) * Tris HClTris6 N HCl ph 88-89
35 35 IEF buffer 1X IEF Sample Buffer ph C Lysine (free base) 40 mm Glycerol Deionized water 15% IEF Sample Buffer ph C Arginine (free base) Lysine (free base) Glycerol Deionized water 20 mm 20 mm 15% IEF Cathode Buffer ph 3-7 (upper buffer chamber) +4 C Lysine (free base) Deionized water 40 mm IEF Cathode Buffer ph 3-10 (upper buffer chamber) +4 C Arginine (free base) Lysine (free base) Deionized water 20 mm 20 mm (ph 10.1) IEF Anode Buffer (for both ph ranges) (lower buffer chamber) Room temperature Phosphoric acid 85% Deionized water 7 mm (ph 2.4) Urea-Thiourea-CHAPS (rehydration buffer for IPG strips) 20 C Deionized urea Deionized thiourea CHAPS Ampholytes* Bromophenol Blue 7 M 2 M 2 4% % 0.002% Ultrapure water DTT 20 mm * ZOOM Strip ph 9-12ampholyte1% ZOOM Focusing Buffer ph 7-12
36 36 MW SDS-PAGE native PAGE
37 37 PageRuler Unstained Low Range Protein Ladder PageRuler Unstained Protein Ladder NativeMark Unstained Protein Standard : 3 : PageRuler Prestained Protein Ladder PageRuler Plus Prestained Protein Ladder Spectra Multicolor Broad Range Protein Ladder HiMark Prestained Protein Standard Spectra Multicolor High Range Protein Ladder : MagicMark XP Western Protein Standard PageRuler Prestained NIR Protein Ladder Bench BenchMark Protein Standard BenchMark His-tagged Protein Standard IEF Marker 3-10
38 38 3. MW PageRuler Unstained Low Range Protein Ladder kda 8 25 kda NA PageRuler Unstained Protein Ladder kda kda NA NativeMark Unstained Protein Standard 20 1,200 kda 8 Native NA PageRuler Prestained Protein Ladder kda kda; 70 kda Good PageRuler Plus Prestained Protein Ladder kda 9 10 kda; 2570 kda Good HiMark Prestained Protein Standard kda 9 Good Spectra Multicolor Broad Range Protein Ladder kda kda; Best kda; 140 kda Spectra Multicolor High Range Protein Ladder kda 8 50 kda; kda Best IEF IEF Marker 3-10 pi pi NA MagicMark XP Western Protein Standard kda 9 NA NIR PageRuler Prestained NIR Protein Ladder kda kda NA BenchMark Fluorescent Protein Standard kda 7 NA His-tag BenchMark His-tagged Protein Standard kda 10 NA sher.com/proteinstandards 90
39 39 Coomassie NA Best NA Good NA Good NA Good NA Best NA Good Good NA Good Good Good NA Good NA Good NA NA Best NA Best NA Best NA Best NA NA Good NA NA NA Good NA Best NA NA NA NA NA NA NA Good NA Good NA Anti-His
40 40 PageRuler Unstained Low Range Protein Ladder Thermo Scientific PageRuler Unstained Low Range Protein Ladder 8 SDS-PAGE Streptag II53.4 kda Strep-Tactin 83.4kDa 100 kda 25 kda Ponceau SCoomassie Thermo Scientifi c Pierce Reversible Protein Stain Kit PageRuler Unstained Low Range Protein Ladder NuPAGE 4 12% Bis-Tris gelmes SDS buffer : Tris-H 3 PO 4 EDTA SDS DTT : 20ºC : 1 PageRuler Unstained Protein Ladder Tris-Glycine gel Bis-Tris gel Tris-Acetate gel sher.com/unstainedstandards 90
41 41 PageRuler Unstained Protein Ladder Thermo Scientific PageRuler Unstained Protein Ladder14 SDS-PAGE Strep-tag II sequence Strep-Tactin conjugate kda kda Coomassie Pierce Reversible PageRuler Unstained Protein Ladder NuPAGE 4 12% Bis-Tris gelmes SDS buffer : Tris-H 3 PO 4 EDTA SDS DTT : 20ºC : 1 PageRulerTris-Glycine gel Bis-Tris gel Tris-Acetate gel
42 42 NativeMark Unstained Protein Standard kda 1,236 1,048 kda 1,236 1, native Invitrogen NativeMark Unstained Protein Standard native NativeMark Unstained Protein Standard NativePAGE Bis-Tris gel 20 1,200 kda 8 Coomassie Ponceau S Coomassie % % : Bis/Tris-HCl ph 7.0NaCl Ponceau S : 20ºC : 6 NativeMark Unstained Protein StandardNativePAGE Bis-Tris gel Novex Tris-Glycine gel NuPAGE Tris-Acetate gel sher.com/unstainedstandards 90
43 43 PageRuler Prestained Protein Ladder Thermo Scientific PageRuler Prestained Protein Ladder 10 SDS-PAGE SDS-PAGE kda kda 10 kda Coomassie PageRuler Prestained Protein Ladder NuPAGE 4 12% Bis-Tris gel MES SDS buffer : Tris-H 3 PO 4 EDTA SDS DTT : 20ºC : 1 PageRuler Prestained Protein Ladder Tris-glycine gel, Bis- Tris gel Tris-acetate gel
44 44 PageRuler Plus Prestained Protein Ladder Thermo Scientific PageRuler Plus Prestained Protein Ladder 9SDS-PAGE SDS-PAGE 70 kda25 kda10 kda 10 kda250 kda 9 Coomassie PageRuler Plus Prestained Protein Ladder NuPAGE 4 12% Bis-Tris gel with MES SDS buffer : Tris-H 3 PO 4 EDTA SDS DTT : 20 C : 1 PageRuler Plus Prestained Protein Ladder Tris-glycine gel Bis-Tris gel Tris-acetate gel sher.com/prestainedstandards 90
45 45 HiMark Prestained Protein Standard kda Invitrogen HiMark Prestained Protein Standard NuPAGE Tris-acetate gel kda HiMark Prestained Protein Standard NuPAGE 3 8% Tris-acetate SDS buffer : Tris-HCl SDS : 20ºC : 6 HiMark Prestained Protein StandardNuPAGE Tris- Acetate gelnupage 4 12% Bis-Tris gelinvitrogen NuPAGE MOPS SDS Running BufferNovex 4% WedgeWell Tris-Glycine gel NuPAGE Tris-Acetate gel HiMark Invitrogen NuPAGE Large Protein Staining Kit Invitrogen NuPAGE Large Protein Sensitive Staining Kit Invitrogen NuPAGE Large Protein Blotting Kit
46 46 Spectra Multicolor Broad Range Protein Ladder Thermo Scientific Spectra Multicolor Broad Range Protein Ladder4 10 SDS-PAGE kda260 kda Coomassie Spectra Multicolor Broad Range Protein Ladder NuPAGE 4 12% Bis-Tris gelmes SDS buffer : Tris-H 3 PO 4 EDTA SDS DTT : 20 C : 1 Spectra Multicolor Broad Range Protein Ladder Tris-glycine gel Bis-Tris gel Tris-acetate gel sher.com/prestainedstandards 90
47 47 Spectra Multicolor High Range Protein Ladder Thermo Scientific Spectra Multicolor High Range Protein Ladder 8 SDS-PAGE 8 40 kda300 kda; 3 Spectra Multicolor High Range Protein Ladder NuPAGE 4 12% Bis-Tris gelmes SDS buffer : Tris-H 3 PO 4 EDTA SDS DTT : 20ºC : 1 Spectra Multicolor High Range Protein LadderTris-glycine gel Bis-Tris gel Tris-acetate gel Coomassie
48 48 MagicMark XP Western Protein Standard MagicMark XP Western Protein Standard IgG 20 kda220 kda 9 kda MagicMark XP Western Protein Standard NuPAGE Bis-Tris gel Invitrogen WesternBreeze Chemiluminescent Kit : Tris-HCl ph 6.8DTT SDS : 20ºC : 4 MagicMark XP Western Protein Standard NuPAGE Bis-Tris gel, Novex WedgeWell Tris-Glycine gel Novex Tricine gel NuPAGE Tris-Acetate gel Bolt Bis-Tris Plus gel sher.com/westernblotstandard 90
49 49 PageRuler Prestained NIR Protein Ladder Thermo Scientific PageRuler Prestained NIR Protein Ladder10 NIR SDS-PAGE 55 kda 11 kda250 kda 10 Typhoon imager LI-COR Odyssey Infrared Imaging System ; PageRuler Prestained NIR Protein Ladder NuPAGE 4 12% Bis-Tris gelmes SDS buffer : Tris-H 3 PO 4 EDTA SDS DTT : 20ºC : 1 PageRuler Prestained NIR Protein Ladder
50 50 BenchMark Fluorescent Protein Standard Invitrogen BenchMark Fluorescent Protein StandardAlexa Fluor 488 BenchMark Fluorescent Protein Standard NuPAGE 4 12% Bis-Tris gelmes SDS buffer 11 kda 155 kda 7 SDS-PAGEUV : Tris-HCl SDS Coomassie G-250 : 20ºC : 6 BenchMark Fluorescent Protein Standard NuPAGE gel Novex WedgeWell Tris-Glycine gel sher.com/specialtystandards 90
51 51 BenchMark His-tagged Protein Standard His Invitrogen BenchMark His-tagged Protein StandardHis 6xHis 10 kda160 kda 10His SDS-PAGEInvitrogen InVision His-Tag In-Gel Coomassie R-250 Anti-His C- SimplyBlue InVision BenchMark His-tagged Protein Standard NuPAGE 4 12% Bis-Tris gelmes SDS buffar : Tris-HCl SDS DTT Coomassie Blue G-250 : 20ºC : 6 BenchMark His-tagged Protein Standard NuPAGE gel Novex WedgeWell Tris-Glycine gel IEF Marker 3-10 IEF Marker 3-10IEF IEF pi IEF Marker 3-10 Novex ph 3 10 gel pi ; : 10% 0.01%0.01% : 20ºC : 1 IEF Marker 3-10IEF gel
52 52 : = V = IR = W = IV Tris-glycine. IEF 90
53 53 Mini Gel Tank XCell SureLock Mini-Cell XCell4 SureLock Midi-Cell 2 28 x 8 cm 4 8 x 13 cm 32 x 11.5 x 16 cm 14 x 13 x 16 cm 21 x 19 x 16 cm x x Mini Gel Tank NuPAGE, Bolt Novex XCell II Blot Mini Blot sher.com/electrophoresischambers
54 54 Mini Gel Tank Mini Gel Tank 2412 Mini Gel Tank : NuPAGE Novex Bolt : 2 L x W x H : 32 x 11.5 x 16 cm : 400 ml : : Mini Gel Tank sher.com/minigeltank sher.com/minigeltank 90
![55 [+] 1. + 1X 2. 1X3 3. 4. 1X 24. Mini Gel 5. FILL 1X 6. 1 25.](/docs-images/92/107909645/images/55-0.jpg "Bolt gel Mini Gel Tank 10 μl Invitrogen Bolt 4 12% Bis-Tris Plus")
55 55 [+] X 2. 1X X 24. Mini Gel 5. FILL 1X Bolt gel Mini Gel Tank 10 μl Invitrogen Bolt 4 12% Bis-Tris Plus gelmini Gel Tank200 V SimplyBlue SafeStain fl atbed scanner 1: SeeBlue Plus2 Prestained Standard; 2: 10 μg E. coli ; 3: Mark12 Unstained Standard 12 ; 4: 40 μg HeLa; 5: 20 μg HeLa ; 6: 5 μg BSA; 7: 40 μg Jurkat; 8: 5 μg GST; 9: Novex Sharp ; 10: 5 μg - Mini Blot ModuleMini Gel Tank PVDF
56 56 XCell SureLock Mini-Cell 2 Invitrogen XCell SureLock Mini-Cell 26 XCell SureLock Mini-Cell NuPAGE Novex Bolt 27 XCell SureLock Mini-Cell: 1 2 : 2 x x: 14 x 13 x 16 cm Novex: : 200 ml : 600 ml : XCell SureLock Mini-Cell sher.com/surelockmini 90
57 XCell SureLock Mini-Cell 1. XCell SureLock Mini-Cell NuPAGE Bis-Tris XCell SureLock Mini-Cell 1: SeeBlue Plus2 Prestained Standard; 2: 10 μg E. coli; 3: Mark12 12 ; 4: 40 μg HeLa; 5: 20 μg HeLa ; 6: ; 7: 40 μg Jurkat; 8: 5 μg GST; 9: Invitrogen Novex Sharp; 10: 5 μg VSimplyBlue SafeStain NuPAGE Bis-Tris XCell SureLock Mini-Cell XCell SureLock Mini-Cell XCell II Blot
58 58 XCell4 SureLock Midi-Cell 4 Invitrogen XCell4 SureLock Midi-Cell1-4 Novex 29 XCell4 SureLock Midi-Cell : 1 4 : 4 8 x 13 cm x x: 21 x 19 x 16 cm : : 175 ml x 4 : ml : sher.com/surelockmidi 90
59 59 A. 1. XCell4 SureLock Midi-Cell XCell4 SureLock Midi-Cell XCell4 Sure- Lock XCell4 SureLock B. 3. A B : XCell4 SureLock Midi-Cell Invitrogen NuPAGE 4 12% Bis-Tris Midi Gel MES XCell4 SureLock Midi Cell 200 VSimplyBlue SafeStain μl Mark12 Unstained Standard μg E. coli μg IgG μgigm μl SeeBlue Plus2 Prestained Protein Standard μl BenchMark μl MagicMark XP Western Protein Standard μl HiMark Unstained Protein Standard
60 60 PowerEase 90W Power Supply Invitrogen PowerEase 90W Power Supply PowerEase 90W Power Supply : - PowerEase 300W Power Supply sher.com/powerease Invitrogen PowerEase 300W Power Supply PowerEase 300W Power Supply:
61 61 4. XCell Surelock Mini-Cell Mini Gel Tank V ma * ma * V ma * ma * Bolt 4 12% MES NA NA NA NA Bolt 4 12% MOPS NA NA NA NA NuPAGE 4 12% Bis-Tris to to MES NuPAGE 4 12% Bis-Tris to to MOPS to 40 8 to Novex WedgeWell 4 20% Tris-Glycine Novex WedgeWell 4 20% Tris-Glycine NuPAGE 3 8% Tris-Acetate NuPAGE 3 8% Tris-Acetate to 12 3 to 6 1 to 12 hours to to to 3 hours Novex 10 20% Tricine NativePAGE 3 12% to 16 2 to 4 90 to <10 80 ph 3-10 IEF NA NA NA NA NA NA NA NA % Zymogram to 40 8 to * :
62 62 XCell SureLock Mini-Cell 61 XCell SureLock Mini Gel 61
63 1X 1X SDS DNA DTT- ; : NuPAGE 12 63
64 64 / Invitrogen SYPRO Orange Red Ruby gel Stain Pierce Reversible stain PVDF Pro-Q Emerald Glycoprotein Stain Pro-Q Diamond Phosphoprotein Stain : DI H2O S DI H2O S : Coomassie Thermo Scientifi c PageBlue stain SimplyBlue SafeStain Thermo Scientifi c Imperial Protein Stain Thermo Scientifi c Pierce Silver Stain Invitrogen SilverXpress Silver Stain Thermo Scientifi c Pierce Silver Stain for Mass Spectrometry sher.com/proteinstains 91
65 65 Coomassie Coomassie G-250 R-250 5Coomassie1 : Coomassie Coomassie SimplyBlue SafeStain PageBlue32Imperial Coomassie 5. Coomassie SimplyBlue Safe Stain Imperial Protein Stain G-250 R-250 G-250 >7 ng 3 ng 5 ng PageBlue Protein Staining Solution : PVDF 3x Coomassie G-250
66 66 DI H2O S DI H2O DI H2O 30. SimplyBlue SafeStain SimplyBlue SafeStain ml ml 1 DI H2O S DI H2O Imperial Protein Stain Imperial Protein Stain DI H2O S DI H2O DI H2O PageBlue Protein Staining Solution 3. 2 < PageBlue Protein Staining Solution 91
67 SimplyBlue SafeStain NuPAGE Novex 4-12% Bis-Tris GelSimplyBlue SafeStain1: 6 μg ; 2: 1 μg IgG; 3: 1 μg BSA; 4: 5 μg E. coli ; 5: 20 ng BSA; 6: 10 ng BSA; 7: 7 ng gbsa; 8: 3 ng BSA; 9: 10 μl Mark12 Unstained Standard 12 ; 10: 5 μl Mark12 Unstained Standard DE ; SimplyBlue SafeStainZOOM IEFpH ZOOM NuPAGE Novex 4 12% Bis-Tris ZOOM SimplyBlue SafeStain Coomassie Rabbit IgG BSA Protein A Protein G Lysozyme 5 ; ; Imperial Protein Stain Imperial11 1: BSA6 μg ; 2 9: 1, ng Thermo Scientific Pierce Power Stainer 2 Coomassie 1 72? Coomassie
68 68 AspGlu His CysLys : 6SilverXpress 37Pierce sher.com/silverstains 91
69 69 6. Pierce Silver Stain Kit for Mass Spectrometry Pierce Silver Stain Kit SilverXpress Silver Staining Kit ng 0.25 ng 0.86 ng 4ºC H 2 O F SZ H 2 O S S 4. 2 H 2 O D D SilverXpress NuPAGE Novex 4-12% Bis-TrisSilverXpress 1 10: 1:4Mark12 Unstained Standard 12; 2: 1:8 Mark12 Unstained Standard; 3: 1:16Mark12 Unstained Standard; 4: 1:32Mark12 Unstained Standard; 5: 64:1Mark12 Unstained Standard; 6: 1.6 ng BSA; 7: 0.8 ng BSA; 8: 1:20E. coli; 9: 1:80E. coli SS H 2 O SilverXpress Silver Staining Kit
70 70 H 2 O EtOH Acetic Acid F F 1. 5x2 2. /15 x2 10% SZ SZ 3. 10%5 x x S S 39. Pierce Silver Stain Kit 0.25 ng D 5% Acetic Acid D % Pierce Silver Stain Kit 91
71 71 1 PAGE Invitrogen SYPRO protein stainpage 7 UV sher.com/fl uorescentstains Polaroid SYPRO Photographic Filter : 2 Coomassie 7. SYPRO SYPRO Ruby stain SYPRO Orange stain SYPRO Red stain 0.25 ng 4 8 ng 4 8 ng 90 ; Ex/Em 280 nm 450/610 nm 300 nm 470/510 nm 300 nm 550/630 nm IEF 2D IEF 2D IEF 2D
72 72 sher.com/specialtystains 8 8. Pro-Q Emerald 488 Glycoprotein Gel and Blot Stain Kit Pro-Q Emerald 300 Glycoprotein Gel and Blot Stain Kit Pro-Q Diamond Phosphoprotein Gel Stain Kit 4 ng 0.5 ng 1 16 ng Ex/Em 510/520 nm 280/530 nm 555/580 nm 91
73 73 Pierce Thermo Scientific Pierce Power Stainer Thermo Scientifi c Pierce Power Station Thermo Scientifi c Pierce Power Stain Cassette Coomassie? Coomassie SDS-PAGE Thermo Scientifi c Pierce Midi Gel Mini Gel Power Staining Kits Pierce Power Stainer 6 Coomassie Pierce + Coomassie R-250 Pierce Power Stainer sher.com/powerstainer
74 74 Pierce Power Stainer Coomassie 10 Thermo Scientifi c Pierce Power Stainer Coomassie Pierce : Coomassie : : SDS-PAGE gel : : Pierce Power System LCD Pierce Power Stainer Coomassie : 11 : / Coomassie * ** 20 x *Coomassie: 45%10%0.25% Coomassie R-250 ** : 30%5% 40. Pierce Power Stainer sher.com/powerstainer 91
75 75 Pierce Power system Coomassie Pierce Power Stainer Pierce Power Blot Cassette Pierce Power system? Coomassie 1 1 Thermo Scientific Pierce Power Stainer Thermo Scientific Pierce Power Blotter
76 76 2 PVDF polyvinylidene difl uoride HRP AP 1 CCD 12 91
77 77 Mini Blot Module Thermo Scientific Pierce Power Blotter iblot 2 Dry Blotting System : X ibind Flex Western Device sher.com/western
78 78 Bolt Mini Gel Bolt Mini Gel Bolt Mini 1X x x Bolt LDS Sample Buffer 4X Bolt 10X 26 μl 30 μl 40 70ºC 10 * 400 ml 1X SDS Running Buffer 20 ml 20X Bolt MES MOPS SDS Running Buffer 380 ml Bolt Mini1-2 * X Running Buffer X Running Buffer X Running Buffer fill1x Fill Line MES 200 V 22 MOPS 200 V 32 * Run times may vary depending upon gel type and power supply. Cathode Cathode NuPAGE Bis-Tris Mini Gel XCell SureLock Mini-Cell 1X NuPAGE LDS Sample Buffer 4X x μl 2.5 μl x μl 2.5 μl NuPAGE10X 1 μl μl 10 μl 10 μl 10 μl 70 C ml 20X NuPAGE MES MOPS SDS Running Buffer 760 ml 1X SDS Running Buffer 200 ml 600 ml 1X Running Buffer200 ml 1X Running500 μl NuPAGE : 200 V : 35MES50MOPS : ma/; ma/ NuPAGE Tris-Acetate Mini Gel 1X * x μl x μl NuPAGE LDS Sample Buffer 4X 2.5 μl -- Tris-Glycine Native Sample Buffer 2X -- 5 μl 10 μl 10 μl 10 μl 10 μl 70 C 10 * NuPAGE10X1X : 40 ml 20X NuPAGE Tris-Acetate SDS Running Buffer 760 ml : 80 ml 10X Tris-Glycine Native Running Buffer 720 ml 200 ml 600 ml 1X Running Buffer200 ml 1X Running Buffer 500 μl NuPAGE Antioxidant : 150 V : 12 3 : ma/; ma/ 18 ma/; 7 ma/
79 79 NuPAGE Bis-Tris Midi Gel XCell4 SureLock Midi-Cell Bis-Tris Gel 1X x μl x μl NuPAGE LDS Sample Buffer 4X 2.5 μl 2.5 μl NuPAGE 10X 1 μl 10 μl 10 μl 70ºC ml 20X NuPAGE MES MOPS SDS Running Buffer 760 ml 1X SDS Running Buffer 175 ml 1X NuPAGE SDS Running Buffer 175 ml 1X NuPAGE SDS Running Buffer 435 μl NuPAGE 1X NuPAGE SDS Running Buffer : 200 V : 40 MES 55MOPS : ma/; ma/ NuPAGE Tris-Acetate Midi Gel 1X Native x μl x μl NuPAGE LDS Sample Buffer 4X 2.5 μl Tris-Glycine Native Sample Buffer 2X 5 μl NuPAGE 10X * 1 μl 10 μl 10 μl * Heat denaturing70ºc 10native : 40 ml 20X NuPAGE Tris-Acetate SDS Running Buffer 760 ml Native : 80 ml 10X Tris-Glycine Native Running Buffer 720 ml 175 ml 1X Running Buffer 175 ml 1X Running Buffer 435 μl NuPAGE Antioxidant Running Buffer : 150 V : : ma/; ma/ ; ma/ ; ma/ ; Novex WedgeWell Tris-Glycine Gels Mini Gel Tank XCell SureLock Mini-Cell 1X Running Buffer x μl x μl Tris-Glycine SDS Sample Buffer (2X) 20 μl 20 μl NuPage (10X) 4 μl 40 μl 40 μl 85ºC 2 Mini Gel Tank: 40mL 10X Novex Tris-Glycine SDS Running Buffer 360mL XCell SureLock Mini-Cell: 100mL 10X Novex Tris-Glycine SDS Running Buffer 900mL Mini Gel Tank: : 225V **: *: mA30-55mA XCell SureLock Mini-Cell: : 225V **: *: 45-60mA30-45mA *Mini Gel2 **
80 80 Tris-Glycine Midi Gel XCell4 SureLock Midi-Cell 1X x μl x μl Tris-Glycine SDS Sample Buffer 2X 5 μl 5 μl NuPAGE 10X 1 μl 10 μl 10 μl 85ºC 2 80 ml 10X Tris-Glycine SDS Running Buffer 720 ml1x Tris- Glycine SDS Running Buffer 175 ml 1X Tris-Glycine SDS Running Buffer fill1x Tris-Glycine SDS Running Buffer : 125 V : 105 : ma/; ma/ 1X Tris-Glycine Native Sample Buffer 2X x μl 5 μl 10 μl 80 ml 10X Tris-Glycine SDS Running Buffer 720 ml1x Tris- Glycine SDS Running Buffer 175 ml 1X Tris-Glycine Native Running Buffer fill1x Tris- Glycine Native Running Buffer : 125 V : 1 12 : ma/; ma/ NativePAGE Bis-Tris Gel NativePAGE Bis-TrisXCell SureLock Mini-Cell Coomassie G-250 NativePAGE gel ~2 360 ng BSA 1X x μl x μl NativePAGE Sample Buffer 4X 2.5 μl 2.5 μl NativePAGE 5% G μl* 10μL 10μL * G-2501/4 1X NativePAGE : 30 ml 20X NativePAGE Running Buffer 570 ml 1X NativePAGE : 10 ml 20X NativePAGE Running Buffer 10 ml 20X NativePAGE 200 ml ml40%10% ml8% watts X NativePAGE Cathode Buffer ~200 ml 1X NativePAGE Cathode Buffer ~550 ml 1X NativePAGE Anode Buffer : : : 150 V %; % ma/; 2 4 ma/
81 81 Tricine Gel XCell SureLock Mini-Cell Tricine x μl x μl Tricine SDS Sample Buffer 2X 5 μl 5 μl NuPAGE 10X 1 μl μl 10 μl 85ºC 2 10 μl 10 μl Tricine Gel Tricine1X Tris-Glycine20 %PVDF XCell II25 V ma Tris-Glycine 1X NuPAGE Transfer Buffer 0.5X TBE NuPAGE Transfer Buffer N 1X 80 ml 10X Novex Tricine SDS Running Buffer 720 ml 1X Tricine SDS Running Buffer 200 ml600 ml 1X Tricine SDS Running Buffer : 125 V : 90 : 80 ma/; 40 ma/ IEF gel XCell SureLock Mini-Cell IEF 1X IEF Sample Buffer ph 3 10 or ph 3 7 2X x μl 5 μl 10 μl 1X IEF: 12 ml 50X IEF Anode Buffer 588 ml 4ºC10ºC 1X IEF: 20 ml IEF Cathode Buffer ph XpH X180 ml 4ºC10ºC 200 ml 1X IEF Cathode Buffer 600 ml 1X IEF Anode Buffer : : 100 V1 200 V1 500 V30 7 ma/; 5 ma/ IEF12% TCA 3.5% 12% TCA 30 IEF IEF gel 2D SDS/ PAGE 1. IEF gel IEF gel 20%10 2. SDS 3. 2 ml 2X SDS 0.5 ml3 5 1X SDS Running Buffer 4. SDS1X SDS Running Buffer 5. IEF gel strip cm 6a. 1.0 mm SDS SDS2DSDS 5.8 cm 4 cmsds Running Buffer 2D-SDS 6b. 1.5 mm SDS 25.8 cm 4 cm1x SDS Running Buffer2 0.5 mm SDS2DSDS 7. 1X SDS Running Buffer SDS-PAGE 8. 10
82 82 Zymogram gel Zymogram gel Zymogram gel XCell SureLock Mini-Cell 1X x μl Tris-Glycine SDS Sample Buffer 2X 5 μl 10 μl Zymogram gel 1X Tris-Glycine SDS Running Buffer: 80 ml 10X Tris-Glycine SDS Running Buffer 720 ml 200 ml600 ml 1X Tris-Glycine SDS Running Buffer : 125 V : 90 : ma/; 8 12 ma/ 1. Zymogram Renaturing Buffer 10XZymogram Developing Buffer 10X 1: ml 1X 2. 1X Zymogram Renaturing Buffer30 3. Zymogram Renaturing Buffer 1X Zymogram Developing Buffer X Zymogram Developing Buffer 6. 37ºC41 Zymogram4 16%Zymogram gelcolloidal Blue Staining Kit SimplyBlue Safestain 10% Zymogramgel: 10-6 unit 12% Zymogramgel: unit 4 16% Zymogramgel: unit
83 83 Ordering information Handcast polyacrylamide gels SureCast Gel Handcast Bundle A Multiple HC1000SR SureCast Gel Handcast Bundle B Multiple HC1000S SureCast Gel Handcast Station SureCast Glass Plates SureCast Sealing Pads SureCast 10-well Multi-Use Tool SureCast 12-well Multi-Use Tool SureCast 15-well Multi-Use Tool 1 casting station 2 glass plate sets (2 front and 2 back) 2 sealing pads 1 multi-use tool 1 multi-use tool 1 multi-use tool HC1000 HC1001 HC1002 HC1010 HC1012 HC1015 SureCast Gel Spacer 10 spacers HC1003 SureCast Stacking Buffer (1 L), 2-pack SureCast Stacking Buffer (2.5 L), 5-pack SureCast Resolving Buffer (1 L), 2-pack SureCast Resolving Buffer (2.5 L), 5-pack 2 x 500 ml dry packs 5 x 500 ml dry packs 2 x 500 ml dry packs 5 x 500 ml dry packs HC2112 HC2115 HC2212 HC2215 SureCast APS 25 g HC2005 SureCast Acrylamide Solution, 40% 450 ml HC2040 SureCast TEMED 30 ml HC2006 Select precast gel Bolt Bis-Tris Plus Gels Bolt 8% Bis-Tris Plus Gels, 10-well 1 box NW00080BOX Bolt 8% Bis-Tris Plus Gels, 12-well 1 box NW00082BOX Bolt 8% Bis-Tris Plus Gels, 15-well 1 box NW00085BOX Bolt 8% Bis-Tris Plus Gels, 17-well 1 box NW00087BOX Bolt 10% Bis-Tris Plus Gels, 10-well 1 box NW00100BOX Bolt 10% Bis-Tris Plus Gels, 12-well 1 box NW00102BOX Bolt 10% Bis-Tris Plus Gels, 15-well 1 box NW00105BOX Bolt 10% Bis-Tris Plus Gels, 17-well 1 box NW00107BOX Bolt 12% Bis-Tris Plus Gels, 10-well 1 box NW00120BOX Bolt 12% Bis-Tris Plus Gels, 12-well 1 box NW00122BOX Bolt 12% Bis-Tris Plus Gels, 15-well 1 box NW00125BOX Bolt 12% Bis-Tris Plus Gels, 17-well 1 box NW00127BOX Bolt 4 12% Bis-Tris Plus Gels, 10-well 1 box NW04120BOX Bolt 4 12% Bis-Tris Plus Gels, 12-well 1 box NW04122BOX Bolt 4 12% Bis-Tris Plus Gels, 15-well 1 box NW04125BOX Bolt 4 12% Bis-Tris Plus Gels, 17-well 1 box NW04127BOX Bolt Empty Mini Gel Cassettes 20 cassettes NW2010 Bolt Empty Mini Gel Cassette Combs, 10-well Bolt Empty Mini Gel Cassette Combs, 12-well 20 combs NW combs NW3012 Bolt Welcome Pack B, 4 12%, 15-well 1 kit** NW0412B Bolt Welcome Pack A, 4 12%, 10-well 1 kit** NW0412A One box contains 10 gels. ** Bolt Welcome Pack kit includes Mini Gel Tank, 2 boxes of Bolt 4 12% Gels (10-well/15-well), Bolt MES Running Buffer (20X), Bolt LDS Sample Buffer (4X), Bolt Sample Reducing Agent (10X) and SeeBlue Plus2 Prestained Standard NuPAGE Bis-Tris Mini Gels (8 cm x 8 cm) NuPAGE Novex 10% Bis-Tris Protein Gels, 1.0 mm, 1-well 1 box NP0304BOX NuPAGE Novex 10% Bis-Tris Protein Gels, 1.0 mm, 10-well NuPAGE Novex 10% Bis-Tris Protein Gels, 1.0 mm, 10-well NuPAGE Novex 10% Bis-Tris Protein Gels, 1.0 mm, 12-well NuPAGE Novex 10% Bis-Tris Protein Gels, 1.0 mm, 12-well NuPAGE Novex 10% Bis-Tris Protein Gels, 1.0 mm, 15-well NuPAGE Novex 10% Bis-Tris Protein Gels, 1.0 mm, 9-well NuPAGE Novex 10% Bis-Tris Protein Gels, 1.5 mm, 10-well NuPAGE Novex 10% Bis-Tris Protein Gels, 1.5 mm, 15-well NuPAGE Novex 12% Bis-Tris Protein Gels, 1.0 mm, 1-well NuPAGE Novex 12% Bis-Tris Protein Gels, 1.0 mm, 10-well NuPAGE Novex 12% Bis-Tris Protein Gels, 1.0 mm, 10-well NuPAGE Novex 12% Bis-Tris Protein Gels, 1.0 mm, 12-well 10 gels NP0301BOX 2 gels NP0301PK2 10 gels NP0302BOX 2 gels NP0302PK2 1 box NP0303BOX 1 box NP0307BOX 1 box NP0315BOX 1 box NP0316BOX 1 box NP0344BOX 10 gels NP0341BOX 2 gels NP0341PK2 10 gels NP0342BOX
84 84 Ordering information NuPAGE Novex 12% Bis-Tris Protein Gels, 1.0 mm, 12-well NuPAGE Novex 12% Bis-Tris Protein Gels, 1.0 mm, 15-well NuPAGE Novex 12% Bis-Tris Protein Gels, 1.0 mm, 17-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 1-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 10-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 10-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 12-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 12-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 15-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 15-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 17-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 17-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.0 mm, 9-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.5 mm, 10-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.5 mm, 10-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.5 mm, 15-well NuPAGE Novex 4 12% Bis-Tris Protein Gels, 1.5 mm, 15-well 2 gels NP0342PK2 1 box NP0343BOX 1 box NP0349BOX 1 box NP0324BOX 10 gels NP0321BOX 2 gels NP0321PK2 10 gels NP0322BOX 2 gels NP0322PK2 10 gels NP0323BOX 2 gels NP0323PK2 10 gels NP0329BOX 2 gels NP0329PK2 1 box NP0327BOX 10 gels NP0335BOX 2 gels NP0335PK2 10 gels NP0336BOX 2 gels NP0336PK2 NuPAGE Bis-Tris Midi Gels (8 cm x 13 cm) NuPAGE Novex 10% Bis-Tris Midi Protein Gels, 12+2-well 1 box WG1201BOX NuPAGE Novex 10% Bis-Tris Midi Protein Gels, 12+2-well, w/adapters NuPAGE Novex 10% Bis-Tris Midi Protein Gels, 20-well NuPAGE Novex 10% Bis-Tris Midi Protein Gels, 20-well, w/adapters 1 box WG1201A 1 box WG1202BOX 1 box WG1202A NuPAGE Novex 10% Bis-Tris Midi Protein Gels, 26-well NuPAGE Novex 10% Bis-Tris Midi Protein Gels, 26-well, w/adapters NuPAGE Novex 4 12% Bis-Tris Midi Protein Gels, 12+2-well NuPAGE Novex 4 12% Bis-Tris Midi Protein Gels, 12+2-well, w/adapters NuPAGE Novex 4 12% Bis-Tris Midi Protein Gels, 20-well NuPAGE Novex 4 12% Bis-Tris Midi Protein Gels, 20-well, w/adapters NuPAGE Novex 4 12% Bis-Tris Midi Protein Gels, 26-well NuPAGE Novex 4 12% Bis-Tris Midi Protein Gels, 26-well, w/adapters NuPAGE Novex 8% Bis-Tris Midi Protein Gels, 12+2-well NuPAGE Novex 8% Bis-Tris Midi Protein Gels, 12+2-well, w/adapters NuPAGE Novex 8% Bis-Tris Midi Protein Gels, 20-well NuPAGE Novex 8% Bis-Tris Midi Protein Gels, 20-well, w/adapters NuPAGE Novex 8% Bis-Tris Midi Protein Gels, 26-well NuPAGE Novex 8% Bis-Tris Midi Protein Gels, 26-well, w/adapters 1 box WG1203BOX 1 box WG1203A 1 box WG1401BOX 1 box WG1401A 1 box WG1402BOX 1 box WG1402A 1 box WG1403BOX 1 box WG1403A 1 box WG1001BOX 1 box WG1001A 1 box WG1002BOX 1 box WG1002A 1 box WG1003BOX 1 box WG1003A Novex WedgeWell Tris-Glycine Mini Gels Novex WedgeWell Welcome Pack Multiple XP0412A (4 12%, 10-well) Novex WedgeWell Welcome Pack Multiple XP0412C (4 12%, 15-well) Novex WedgeWell Welcome Pack Multiple XP0010A (10%, 10-well) Novex WedgeWell Welcome Pack Multiple XP0010C (10%, 15-well) Novex WedgeWell 6% Tris-Glycine Mini 10 per box XP00060BOX Gel, 10-well Novex WedgeWell 6% Tris-Glycine Mini 10 per box XP00062BOX Gel, 12-well Novex WedgeWell 6% Tris-Glycine Mini 10 per box XP00065BOX Gel, 15-well Novex WedgeWell 8% Tris-Glycine Mini Gel, 10-well 10 per box XP00080BOX
85 85 Novex WedgeWell 8% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 8% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 10% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 10% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 10% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 10% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 12% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 12% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 12% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 14% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 14% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 14% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 16% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 16% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 16% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 18% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 18% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 18% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 4 12% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 4 12% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 4 12% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 4 12% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 4 20% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 4 20% Tris-Glycine Mini Gel, 12-well 10 per box XP00082BOX 10 per box XP00085BOX 10 per box XP00100BOX 10 per box XP00102BOX 10 per box XP00105BOX 2 per box XP00100PK2 10 per box XP00120BOX 10 per box XP00122BOX 10 per box XP00125BOX 10 per box XP00140BOX 10 per box XP00142BOX 10 per box XP00145BOX 10 per box XP00160BOX 10 per box XP00162BOX 10 per box XP00165BOX 10 per box XP00180BOX 10 per box XP00182BOX 10 per box XP00185BOX 10 per box XP04120BOX 10 per box XP04122BOX 10 per box XP04125BOX 2 per box XP04120PK2 10 per box XP04200BOX 10 per box XP04202BOX Novex WedgeWell 4 20% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 4 20% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 8 16% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 8 16% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 8 16% Tris-Glycine Mini Gel, 15-well Novex WedgeWell 10 20% Tris-Glycine Mini Gel, 10-well Novex WedgeWell 10 20% Tris-Glycine Mini Gel, 12-well Novex WedgeWell 10 20% Tris-Glycine Mini Gel, 15-well 10 per box XP04205BOX 2 per box XP04200PK2 10 per box XP08160BOX 10 per box XP08162BOX 10 per box XP08165BOX 10 per box XP10200BOX 10 per box XP10202BOX 10 per box XP10205BOX NuPAGE Tris-Acetate Mini Gels (8 cm x 8 cm) NuPAGE Novex 3 8% Tris-Acetate Protein Gels, 1.0 mm, 10-well 10 gels EA0375BOX NuPAGE Novex 3 8% Tris-Acetate Protein Gels, 1.0 mm, 10-well NuPAGE Novex 3 8% Tris-Acetate Protein Gels, 1.0 mm, 12-well NuPAGE Novex 3 8% Tris-Acetate Protein Gels, 1.0 mm, 12-well NuPAGE Novex 3 8% Tris-Acetate Protein Gels, 1.0 mm, 15-well NuPAGE Novex 3 8% Tris-Acetate Protein Gels, 1.5 mm, 10-well NuPAGE Novex 3 8% Tris-Acetate Protein Gels, 1.5 mm, 15-well NuPAGE Novex 7% Tris-Acetate Protein Gels, 1.0 mm, 10-well NuPAGE Novex 7% Tris-Acetate Protein Gels, 1.0 mm, 12-well NuPAGE Novex 7% Tris-Acetate Protein Gels, 1.0 mm, 15-well NuPAGE Novex 7% Tris-Acetate Protein Gels, 1.5 mm, 10-well NuPAGE Novex 7% Tris-Acetate Protein Gels, 1.5 mm, 15-well 2 gels EA0375PK2 10 gels EA03752BOX 2 gels EA03752PK2 1 box EA03755BOX 1 box EA0378BOX 1 box EA03785BOX 1 box EA0355BOX 1 box EA03552BOX 1 box EA03555BOX 1 box EA0358BOX 1 box EA03585BOX NuPAGE Tris-Acetate Midi Gels (8 cm x 8 cm) NuPAGE Novex 3 8% Tris-Acetate Midi Protein Gels, 12+2W 1 box WG1601BOX NuPAGE Novex 3 8% Tris-Acetate Midi Protein Gels, 12+2W, w/adapters 1 box WG1601A
86 86 Ordering information NuPAGE Novex 3 8% Tris-Acetate Midi Protein Gels, 20W NuPAGE Novex 3 8% Tris-Acetate Midi Protein Gels, 20W, w/adapters NuPAGE Novex 3 8% Tris-Acetate Midi Protein Gels, 26W NuPAGE Novex 3 8% Tris-Acetate Midi Protein Gels, 26W, w/adapters 1 box WG1602BOX 1 box WG1602A 1 box WG1603BOX 1 box WG1603A Novex Tris-Glycine Mini Gels (8 cm x 8 cm) Novex 10% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well 1 box EC6075BOX Novex 10% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well - Value Pack Novex 10% Tris-Glycine Mini Protein Gels, 1.0 mm, 12-well Novex 10% Tris-Glycine Mini Protein Gels, 1.0 mm, 15-well Novex 10% Tris-Glycine Mini Protein Gels, 1.5 mm, 10-well Novex 10% Tris-Glycine Mini Protein Gels, 1.5 mm, 15-well Novex 10 20% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well Novex 10 20% Tris-Glycine Mini Protein Gels, 1.0 mm, 12-well Novex 10 20% Tris-Glycine Mini Protein Gels, 1.0 mm, 15-well Novex 10 20% Tris-Glycine Mini Protein Gels, 1.5 mm, 15-well Novex 12% Tris-Glycine Mini Protein Gels, 1.0 mm, 1-well Novex 12% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well Novex 12% Tris-Glycine Mini Protein Gels, 1.0 mm, 12-well Novex 12% Tris-Glycine Mini Protein Gels, 1.0 mm, 15-well Novex 12% Tris-Glycine Mini Protein Gels, 1.5 mm, 10-well Novex 12% Tris-Glycine Mini Protein Gels, 1.5 mm, 15-well Novex 14% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well 3 boxes EC6075BX30 1 box EC60752BOX 1 box EC60755BOX 1 box EC6078BOX 1 box EC60785BOX 1 box EC6135BOX 1 box EC61352BOX 1 box EC61355BOX 1 box EC61385BOX 1 box EC6001BOX 1 box EC6005BOX 1 box EC60052BOX 1 box EC60055BOX 1 box EC6008BOX 1 box EC60085BOX 1 box EC6485BOX Novex 14% Tris-Glycine Mini Protein Gels, 1.0 mm, 12-well Novex 14% Tris-Glycine Mini Protein Gels, 1.0 mm, 15-well Novex 14% Tris-Glycine Mini Protein Gels, 1.5 mm, 10-well Novex 14% Tris-Glycine Mini Protein Gels, 1.5 mm, 15-well Novex 16% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well Novex 16% Tris-Glycine Mini Protein Gels, 1.0 mm, 12-well Novex 16% Tris-Glycine Mini Protein Gels, 1.0 mm, 15-well Novex 16% Tris-Glycine Mini Protein Gels, 1.5 mm, 10-well Novex 16% Tris-Glycine Mini Protein Gels, 1.5 mm, 15-well Novex 18% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well Novex 18% Tris-Glycine Mini Protein Gels, 1.0 mm, 12-well Novex 18% Tris-Glycine Mini Protein Gels, 1.0 mm, 15-well Novex 18% Tris-Glycine Mini Protein Gels, 1.5 mm, 10-well Novex 18% Tris-Glycine Mini Protein Gels, 1.5 mm, 15-well Novex 4% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well Novex 4% Tris-Glycine Mini Protein Gels, 1.0 mm, 12-well Novex 4% Tris-Glycine Mini Protein Gels, 1.5 mm, 10-well Novex 4% Tris-Glycine Mini Protein Gels, 1.5 mm, 15-well Novex 4 12% Tris-Glycine Mini Protein Gels, 1.0 mm, 10-well Novex 4 12% Tris-Glycine Mini Protein Gels, 1.0 mm, 12-well Novex 4 12% Tris-Glycine Mini Protein Gels, 1.0 mm, 15-well 1 box EC64852BOX 1 box EC64855BOX 1 box EC6488BOX 1 box EC64885BOX 1 box EC6495BOX 1 box EC64952BOX 1 box EC64955BOX 1 box EC6498BOX 1 box EC64985BOX 1 box EC6505BOX 1 box EC65052BOX 1 box EC65055BOX 1 box EC6508BOX 1 box EC65085BOX 1 box EC6055BOX 1 box EC60552BOX 1 box EC6058BOX 1 box EC60585BOX 1 box EC6035BOX 1 box EC60352BOX 1 box EC60355BOX
Novex Brochure_J_1220.indd
& Novex 7iBlot BenchPro 4100 2 5 11 12 14 16 18 20 21 22 23 3 NuPAGE 5 Bolt & 8 10 11 NuPAGE 5 Bolt 8 10 11 2 New 1.5 200 BT* 30 400 TA* 12 BT* 8 TA* 0.3-2 6 500 6 500 16 1 2 4-25 4-25 2-8 2-8 35 min 35
<835E8393836E3F834E8EBF93648B43896A93AE834A835E838D834E3F5F3135303532322D332E706466>
protein biology & & WESTERN WORKFLOW Contents 2 Separate NuPAGE 3 NuPAGE 4 Bolt & PowerEase 5 6 7 Transfer Bolt 8 2 9 Detect 10 11 MY 12 13 3.0 14 3 www.lifetechnologies.com/western Separate 180 2 Invitrogen
研究者を笑顔にするウェスタン解析ツール Changing the face of westerns ウェスタンブロッティング解析ワークフロー Complete, innovative western workflow solutions
研究者を笑顔にするウェスタン解析ツール Changing the face of westerns ウェスタンブロッティング解析ワークフロー Complete, innovative western workflow solutions Easy! Fast! Clear! Western drama STEP 1 Western done! Separate Slow western STEP 2
DSLshinnendo_ ob0403.indd
Discover Life Science Products and Services 2019 4 19 2019 6 28 P2 4 P5 6 P7 P8 P9 P10 12 PCRP13 16 PCRP16 21 P22 23 &P24 26 P27 30 P31 18! 18 ProLong Glass / SlowFade 30% OFF Invitrogen Molecular Probes
3 1 2
Agilent 4200 TapeStation Agilent 4200 TapeStation 3 1 2 2 3 ScreenTape 3 Agilent 4200 TapeStation QC Agilent 4200 TapeStation Ready-to-use ScreenTape 8 PCR 96-well plate 1 96 1 qrt-pcr DNA RNA DNA / RNA
ROWCatPathology_J.book
www.dako.jp IHC Solution Visualization Systems Number of Steps 5 4 3 2 1 LSAB TM 2 EnVision TM G 2 Doublestain EnVision TM DuoFLEX Doublestain LSAB TM + EnVision TM G 2 EnVision TM + EnVision TM FLEX 1
2
1 2 3 4 5 (written by Dr. ) Stock Solution (one liter) in 4 Buffer A: 0.05 M glucose 20% (1.1 M) glucose 22.7 ml 0.025 M Tris-Cl ph8 1 M Tris (ph8.0) 12.5 ml 0.01 M EDTA 0.5 M EDTA 10 ml Buffer B: 5 M
PG1571-PJT1363-COL01356-MP T indd
Marketplace Thermo Scientific ALL PCR DNA メ ー PCR 製品 dntp PCR ー ン キ Essentials Tour 2016 キャンペーン ー DNA ー 製キ 2016 年 7 月 1 日 2016 年 9 月 30 日 まで キャンペーンの はこちらをご覧ください www.thermofisher.com/jp-mktp PCR Thermo
TaKaRa Bradford Protein Assay Kit
研究用 TaKaRa Bradford Protein Assay Kit 説明書 v201701da TaKaRa Bradford Protein Assay Kit は Coomassie Dye を用いる Bradford 法に基づいたキットであり 簡単な操作で迅速に 濃度範囲が 1 ~ 1,000 μg/ml のタンパク質溶液の定量を行うことができます 本キットの定量の原理は Coomassie
RT-PCR プロトコール.PDF
Real -Time RT-PCR icycler iq Bio Rad RT-PCR RT-PCR 1 icycler iq Bio Rad icycler iq 30 2 Ready-To-Go T-Primed First-Strand Kit (amersham pharmacia biotech) Ready-To-Go T-Primed First-Strand Kit QuantiTect
PTM PTM Cell Signaling Technology CST 1,000 CST CST p.13 CST CST 2
A Guide to successful Western Blotting from Cell Signaling Technology www.cstj.co.jp WB PTM PTM Cell Signaling Technology CST 1,000 CST CST p.13 CST CST www.cstj.co.jp/wbprotocol 2 1 tissue or cells +
QIAquick Spin プロトコールとトラブルシューティング ( /2008)
March 2008 QIAquick Spin QIAquick PCR Purification Kit PCR 100 bp 10 kb QIAquick Nucleotide Removal Kit 17 40mer DNA 40 bp 10 kb QIAquick Gel Extraction Kit DNA 70 bp 10 kb Sample & Assay Technologies
[PDF] Ettan Spot Picker 簡易マニュアル
![[PDF] Ettan Spot Picker 簡易マニュアル](/thumbs/96/126831902.jpg "[PDF] Ettan Spot Picker 簡易マニュアル")
Ettan Spot Picker 71-3703-31 1 Ettan Spot Picker MALDI - TOF Spot Picker (Coomassie) SYPRO ImageMaster 2D Elite 1 X- Y, Spot Picker mm 96 Ettan Spot Picker 8-18% 9600 1.1 Ettan Spot Picker Ettan Spot
TaKaRa BCA Protein Assay Kit
研究用 TaKaRa BCA Protein Assay Kit 説明書 v201307da TaKaRa BCA Protein Assay Kit は 高感度にタンパク質溶液の比色定量を行う試薬であり 界面活性剤によって可溶化されたタンパク質溶液の定量も可能です BCA によるタンパク質定量の原理は 2 段階の反応に基づいています 第 1 段階では タンパク質溶液中のペプチド結合によって キットに含まれる二価銅イオン
[PDF] Typhoonカタログ
![[PDF] Typhoonカタログ](/thumbs/92/108545061.jpg "[PDF] Typhoonカタログ")
GE imagination at work www.gehealthcare.co.jp/lifesciences 71-1891-02 Typhoon Typhoon Molecular Dynamics 1989 PhosphorImager 1992 CLSM 1 View it all from your benchtop 2000 Gen III System 1998 MegaBACE
MACS機器予算申請カタログ
MACS Instruments MACSQuant automacs MultiMACS gentlemacs MACSmix TM MACSQuant 3 MACSQuant Analyzer 10 MACSQuant VYBMACSQuant X MACSQuant X Coming soon! 384 well plate Laser Channel Filter (nm) Dye or Parameter
3 1 Amersham Imager 680 RGB 94 Amersham Imager 680 QC 94 LED LED OD OD Amersham Imager 680 UV 94 Amersham Imager ImageQuant LAS LED LED
3 92 93 94 98 99 101 Ettan 102 104 106 108 109 110 111 102 103 / 107 / 112 Amersham Imager 680 4,739,200 94 ImageQuant LAS 500 2,573,000 98 Amersham Typhoon scanner RI 10,375,800 99 Melanie 971,500 102
PG1571-PJT1690-COL02509-MP T2_J.indd
molecular biology Marketplace MOLECULAR BIOLOGY GREATEST HITS IPTG X-Gal DNA DNA PCR dntp Thermo Scientific off Essentials Tour 2016 2016 10 3 2016 12 22 www.thermofisher.com/marketplace Thermo Scientific
A, B 2 B 5 A C A ADP A G ADP ADP G camp B GM A B CTB Tochikubo et al., 1998 CTB CTB Dertzbaugh & Elson, 1993a; Yu et al., 1994; Shi et al., 1995 CTB C
2004 Analysis of the expression of cholera toxin B-subunit gene 1065010 2004 8 26 1 A, B 2 B 5 A C A ADP A G ADP ADP G camp B GM A B CTB Tochikubo et al., 1998 CTB CTB Dertzbaugh & Elson, 1993a; Yu et
AllPrep DNA/RNA Micro プロトコールとトラブルシューティング ( /2008)
July 2007 AllPrep DNA/RNA Micro DNA RNA 5 x 10 5 5 mg Sample & Assay Technologies DNA RNA 3 DNA RNA 11 DNA RNA 19 24 2 AllPrep DNA/RNA Micro 07/2007 DNA RNA RNA AllPrep DNA Spin Column DNA RNeasy MinElute
Microsoft Word - iBind Western System 日本語簡易取扱説明書
簡易取扱説明書 ibind Western System 簡易取扱説明書 ver.2 ibind Western System 日本語簡易取扱説明書 ver.2 サンプルブロッティング済みメンブレン 試薬 ibind Solution kit 抗体反応用試薬 ibind Detection Kits 検出試薬 (AP 用の二次抗体も含む ) 一次抗体脱イオン水装置と器具 ibind Device ibind
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF Vol. 32, pp , 2004 FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF Vol. 32, pp. 47 51, 2004 FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF 2D-DIGE 21 DNA 2 Sypro Ruby 15 two-dimensional
untitled
E-mail: khatano@fms.saitama-u.ac.jp Tel & Fax: 048-858-3535 Toxin Virus Bacterium Glycolipid Glycoprotein Vero toxin (Stx1 and Stx2) Side view Bottom view H H H H N H Grobotriaosyl Ceramide (Gb 3 : Galα1-4Galβ1-4Glcβ-Cer)
Aqua Way Philic Aqua Way Cation Technology platform Cell-sheet engineering (1) Technology platform Cell-sheet engineering (2) 1) Collection of cells in a form of thin film 2) o suture required for transplantation
NanoVuePlus_H1-4_CC2014.indd
NanoVue Plus 71-3327-34 ... 2... 2... 2... 3... 4... 5... 6 NanoVue Plus...6 NanoVue Plus Sample... 7 Life Science... 9 1. DNA/RNA... 10 2. CyDye... 12 3.... 15 3-1Protein UV UV Protein A280 A280... 15
バイオ解析チーム教育セミナーMS04
LTQ Orbitrap Shotgun proteomics Large scale proteomics by orbitrap technology. Thermo fisher scientific K.K SID C&MS Application OMICS Team TMT 2 Proteome Cell and Tissue Protein Peptide Extraction Protease
[PDF] 2013年度 冬のラボ 充実キャンペーン 第2弾
![[PDF] 2013年度 冬のラボ 充実キャンペーン 第2弾](/thumbs/97/130735069.jpg "[PDF] 2013年度 冬のラボ 充実キャンペーン 第2弾")
冬の 冬のラボ 得 2 ペ ン 第 充実キャン ー 2013年度 ラ ボ 弾 充実キャンペーン 汎用タイプの シリンジフィルター プラディスク シリンジフィルター 20 % OFF PVDF メンブレン Hybond-P ウェスタンブロッティング 検出試薬 ECL Prime ÄKTAdesignの メンテナンスパーツは 10% OFF プレパックカラム HiTrap シリーズ 冬のラボ 充実キャンペーン
untitled
DC Fan 8mm sq. General Specifications San Ace 8 15mm thick (GA type), 15mm thick, 2mm thick (GA type) 2mm thick, 25mm thick (GA type), 25mm thick (S type) 25mm thick (GV type), 25mm thick 25mm thick (San
Product Description Recombinant tagged proteins are widely used for protein purification and protein characterization. There are many purification met
Printed June 27, 2017 Version 3.0 For Research Use Only. Not for use in diagnostic procedures. DDDDK-tagged Protein PURIFICATION CARTRIDGE (Clone FLA-1) CODE No. 3326K PURIFICATION to maintain protein
Product Description The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require h
Printed May 9, 2012 Version 1.0 For Research Use Only. Not for use in diagnostic procedures. HA-tagged Protein Purification Gel (MoAb. clone 5D8) Code No. 3321 Purification to Maintain Protein Activity
untitled
1 2 3 4 Alteration of program parameters Effect Increase hybridization temperature Can prevent false positive signals, decrease background, can also increase dynamic range Decrease hybridization temperature
研究成果報告書(基金分)
DNA cdna mrna DNA ( /) (PS) (PS-tag: RIIIRRIRR) PS scfv PS-tag PS-tag scfv (PMMA)(PC) -S- (GST)PC PMMA GST GST PMMA-tag: DVEGIGDVDLVNYFEVGAT- YTFNK PC-tag: NSNFFGLVDGLNF- AVQYLGK) -S- PS-tag PMMA-tagPC-tag
Product Description Recombinant tagged proteins are widely used for protein purification and protein characterization. There are many purification met
Printed June 20, 2013 Version 1.0 For Research Use Only. Not for use in diagnostic procedures. c-myc-tagged Protein PURIFICATION CARTRIDGE (Clone 1G4) CODE No. 3306K PURIFICATION to maintain protein activity
SEC (SEC) SEC SEC SEC 2
SEC (SEC) SEC SEC SEC 2 SEC ( 1) 1: SEC www.agilent.com/chem/jp 3 ( ) ( 2) ICH (Q6B) 3 lgg 8 7 6 5 4 1. 2. 3. 4. 5. Agilent AdvanceBio SEC 3 Å 7.8 x 3 mm 2.7 µm (p/n PL118-531) Agilent 126 Infinity LC
EVOS FL Auto (W x D x H cm) 33 x (kg) 16 kg LCD Display (inch) 23 ( ) & 1.3 MP CMOS / 3.2 MP CMOS! PC Z- LED XY 4 ( ) EVOS FL Auto 2 Ima
Invitrogen EVOS EVOS XL Core EVOS XL EVOS FLoid EVOS FL EVOS FLAuto 2 EVOS Onstage Incubator 201610 EVOS FL Auto 2 5 + 4 (W x D x H cm) 33 x 46 36 (kg) 16 kg LCD Display (inch) 23 ( ) & 1.3 MP CMOS / 3.2
DynalTransplantDiagnostic.indd
2011 1 SeCore HLA SBT 1 2 AllSet + Gold SSP HLA 7 3 SSP UniTray HLA 11 4 27 5 32 ii 1 SeCore HLA SBT SeCore 2 SeCore HLA SBT SeCore 3 SeCore 4 HLA-I 5 HLA-II 5 utype 5 GSSP 6 1 SeCore HLA SBT Sequencing
プロトコール集 ( 研究用試薬 ) < 目次 > 免疫組織染色手順 ( 前処理なし ) p2 免疫組織染色手順 ( マイクロウェーブ前処理 ) p3 免疫組織染色手順 ( オートクレーブ前処理 ) p4 免疫組織染色手順 ( トリプシン前処理 ) p5 免疫組織染色手順 ( ギ酸処理 ) p6 免疫
< 目次 > 免疫組織染色手順 ( 前処理なし ) p2 免疫組織染色手順 ( マイクロウェーブ前処理 ) p3 免疫組織染色手順 ( オートクレーブ前処理 ) p4 免疫組織染色手順 ( トリプシン前処理 ) p5 免疫組織染色手順 ( ギ酸処理 ) p6 免疫組織染色手順 ( ギ酸処理後 マイクロウェーブまたはオートクレーブ処理 )p7 抗原ペプチドによる抗体吸収試験 p8 ウエスタン ブロッティング
Product Description The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require h
Printed August 22, 2016 Version 3.0 For Research Use Only. Not for use in diagnostic procedures. DDDDK-tagged Protein PURIFICATION GEL (MoAb. clone FLA-1) CODE No. 3329 PURIFICATION to Maintain Protein
Product Description The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require h
Printed November 11, 2016 Version 2.0 For Research Use Only. Not for use in diagnostic procedures. DDDDK-tagged Protein PURIFICATION GEL with Elution Peptide (MoAb. clone FLA-1) CODE No. 3326 / 3327 PURIFICATION
HotStarTaq Plus PCRプロトコールとトラブルシューティング( /2008)
February 2008 HotStarTaq Plus PCR HotStarTaq Plus DNA Polymerase HotStarTaq Plus Master Mix Kit PCR HotStarTaq Plus DNA Polymerase PCR 2 HotStarTaq Plus DNA Polymerase Q-Solution PCR 6 HotStarTaq Plus
Amnis TM CellStream TM CellStream TM
Amnis TM CellStream TM CellStream TM Amnis TM CellStream TM Amnis TM CCD FITC PE 5 96 7 CFR Part FDA TM CellStream Luminex Time Delay Integration TDI CellStream. 7... CCD CellStream Amnis CCD CellStream
Microsoft Word - タンパク質溶液内酵素消化 Thermo
タンパク質の溶液内酵素溶液内酵素消化 ( 質量分析用サンプル調製 ) 質量分析計によるタンパク質解析においては 一般的にタンパク質を還元 アルキル化した後にトリプシン等で酵素消化して得られた消化ペプチドサンプルが用いられます 本資料ではこのサンプル調製について 専用キットを用いて行う方法 各種試薬や酵素を用いて行う方法 また関連情報として タンパク質の定量法についてご紹介しています 内容 1 培養細胞の酵素消化
能書単頁9[1].5(2)
![能書単頁9[1].5(2)](/thumbs/87/96620151.jpg "能書単頁9[1].5(2)")
ips CytoTune -ips ver.1.0 1 I CytoTune ips 3 II 3 3 4 5 III CytoTune ips ips 6 6 6 6 7 1) 2) ips 8 ips 8 SeV 9 SeV 10 IV Q&A 11 V 12 12 VI...13 13...13 2 I CytoTune ips CytoTune ips 4 OCT3/4SOX2 KLF4c
pc725v0nszxf_j
PC725NSZXF PC725NSZXF PC725NSZXF PC725 DE file PC725 Date Jun. 3. 25 SHARP Corporation PC725NSZXF 2 6 5 2 3 4 Anode Cathode NC Emitter 3 4 5 Collector 6 Base PC725NSZXF PC725YSZXF.6 ±.2.2 ±.3 SHARP "S"
steponeplus_bro_f-0210.indd
StepOnePlus / StepOne PCR PCR Applied Biosystems StepOnePlus StepOne PCR 1 Features At A Glance StepOne StepOnePlus / 48 96 FAM/SYBR Green dyes VIC/JOE dyes ROX dyes NED/TAMRA dyes 1. VeriFlex Block StepOnePlus
先端生物工学演習Ⅱ 「タンパク質の電気泳動」
先端生物工学演習 Ⅱ タンパク質の電気泳動 2008 年 10 月 14 日 ( 旧 ) 進化生命システム学塚田幸治 1 話の中身 電気泳動という手法 ( 一般 ) アミノ酸の電荷とタンパク質の電荷 具体的な実験手法について ( 原理 ) Native( ( 構造や機能が破壊されていないタンパク質 ) SDS( ( 変性条件下でのタンパク質 ) 応用例 等電点電気泳動法と二次元電気泳動 非特異的染色法と特異的染色法
[PDF] Alkphos Directマニュアル
![[PDF] Alkphos Directマニュアル](/thumbs/95/124470865.jpg "[PDF] Alkphos Directマニュアル")
AlkPhos Direct ECL Direct 71-1752-32 AlkPhos Direct ECL Direct APHRP 30 AlkPhos Direct APECL Direct HRP2 CONTENTS AlkPhos Direct ECL Direct 1 AlkPhos Direct 5 ECL Direct 29 55 56 57 AlkPhos Direct ECL
1.WizardPlusSV日本語ﾌﾟﾛﾄｺｰﾙ
Wizard Plus SV Minipreps DNA Purification System No. TB281J 2001 7 A1270, A1330, A1340, A1460 A1470 Wizard Plus SV Minipreps DNA Purification System (a) DNA 30 20,000bp DNA Recombinant RNasin Ribonuclease
wako_Spark_v.1.3_Aug2017_j.pdf
Spark R R R 02 SPARK BROCHURE The fully flexible detection platform with smart automation. Made for discovery, today and tomorrow. Spark High Speed Monochromator HSM NanoQuant Plate ELISA DNA/ Spark Premium
Agilent Automated Solutions: AAS Bravo Vertical Pipetter 2 BenchCel Microplate Handler 360 Handling Direct Drive Robot BioCel Agilent VWorks Automatio
アジレント 自動化ソリューション 総合カタログ Automation Solutions Agilent Automated Solutions: AAS Bravo Vertical Pipetter 2 BenchCel Microplate Handler 360 Handling Direct Drive Robot BioCel Agilent VWorks Automation Control
橡documents\TBD015J.PDF
Matrix FL-JOE-TMR-CXR No.TBD015200111 DG2860 ABI PRISM 310 Genetic Analyzer ABI PRISM 377 DNA Sequencer Project ABI PRISM 310 Genetic Analyzer ABI PRISM 377 DNA Sequencer CE 4 Matrix FL-JOE-TMR-CXR 4 DNA
UM PM Q24 ( /2013)
July 2011 PyroMark Q24 Sample & Assay Technologies 1 User Manual 1 Safety Information 2 User Manual 2 Introduction 3 User Manual 3 General Description 4 4 4.1 4 4.2 4 5 5 5.1 5 5.1.1 5 5.1.2 5 5.1.3 5
Product Description The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require h
Printed December 5, 2016 Version 2.0 For Research Use Only. Not for use in diagnostic procedures. His tagged Protein PURIFICATION GEL (MoAb. clone 2D8) CODE No. 3311 / 3312 PURIFICATION to Maintains Protein
mRNA-Seq_SamplePrep.book
mrna Sequencing Sample Preparation Guide Topics 3 Introduction 4 RNA Input Recommendations 6 mrna-seq Sample Preparation Kit Contents 8 User-Supplied Consumables and Equipment 9 Purify the mrna 12 Fragment
Product Description The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require h
Printed August 09, 2011 Version 1.2 For Research Use Only. Not for use in diagnostic procedures. His tagged Protein PURIFICATION GEL (MoAb. clone 2D8) CODE No. 3311 / 3312 PURIFICATION to Maintains Protein
/
333 344 / 332 03-6264-066003-6264-0663 03-6264-0620 334 LAL 335 336 LAL 338 QCL-1000 LAL 33 LAL 340 C 341 LAL 343 ELx808 344 FLx800 345 PyroTec 346 WinKQCL 347 CSE 348 / 03-6264-066003-6264-0663 03-6264-0620
宇宙環境利用蛋白質結晶生成応用利用実証プロジェクトのご案内
16 8 23 1. 14 17 ISS JEM (JAXA) 2. JAXA 14 6 1 4 1 02/02/2003 29/08/2003 29/01/2004 11/08/2004 Baikonur Kazakhstan Progress 03/05/2003 28/10/2003 29/04/2004 19/10/2004 Kazakhstan Soyuz 13 weeks 9 weeks
AriaMx PCR AriaMx PCR PCR AriaMx PCR 0.2 ml 96 6 PCR Ct SYBR Green I PCR Tm TaqMan PCR DNA HRM TaqMan AriaMx 8 Multiple Experiment Analysis PDF RDML P
AriaMx PCR See Deeper. Reach Further. AriaMx PCR AriaMx PCR PCR AriaMx PCR 0.2 ml 96 6 PCR Ct SYBR Green I PCR Tm TaqMan PCR DNA HRM TaqMan AriaMx 8 Multiple Experiment Analysis PDF RDML PCR ROX Rn MIQE
エピトープタグ抗体製品リスト PM020 Anti-DDDDK-tag pab Polyclonal Rabbit Ig (aff.) WB, IP, IC 100 ml 15,000 PM020-7 Anti-DDDDK-tag pab HRP-DirecT Polyclonal Rabbit I
(kda) 150 100 DDDDK-tagged protein 75 50 37 IgG Heavy chain 25 20 IgG Light chain Sample: N-terminal DDDDK-tagged β-gal protein transfectant μ μ μ http://ruo.mbl.co.jp/ エピトープタグ抗体製品リスト PM020 Anti-DDDDK-tag
エッペンドルフ製品価格表
エッペンドルフ製品価格表 2019 1 2 2 3 3 ept.i.p.s. 3 ept.i.p.s. 4 ept.i.p.s. 4 ept.i.p.s. 4 ept.i.p.s. Biopur 4 ept.i.p.s. Biopur 4 ep Dualfilter T.I.P.S. 4 ept.i.p.s. LoRetention 5 5 5 6 6 6 6 7 7 7 epmotion 9 9-10
30% OFF & 31 Eppendorf TC 30% OFF Cell Imaging Dishes 145/170 µm Cell Imaging Coverglasses & Slides 1/2/4/8 chamber > 2 mm 750 µl > 170 µm 145 µ
2016 8 16 10 31 Protect Your Cells from Contamination! 2016 > 30% OFF & 31 > 3 25% OFF info@eppendorf.jp 30% OFF & 31 Eppendorf TC 30% OFF 3 1 8 Cell Imaging Dishes 145/170 µm Cell Imaging Coverglasses
/ Motor Specifications Direct Motor Drive Ball Screws / Precision Ball Screw type MB / MB MB Precision Ball Screw type MB / MoBo C3 5 5 Features A 5-p
/ Motor Specifications MB Precision Ball Screw type MB / MoBo C3 5 5 Features A 5-pahse Stepping Motor is mounted directly onto the shaft end of a C3 grade precision Ball Screw, which is suitable for high
Contents 1 1. ph ph ph 7 a) ph 9 b) 10 c) ph 11 a) 11 b) 12 c) ph 14 d) 15 ph a) 18 b) / 19 c)
ph Theory Guide School experiments ph Natural science laws experience ph live ph learn easily Contents 1 1. ph 5 1.1. 5 1.2. ph 6 1.3. ph 7 a) ph 9 b) 10 c) 11 1.4. ph 11 a) 11 b) 12 c) ph 14 d) 15 ph
Options Unit : mm Finger guards Color Model Surface treatment Nickel-chrome plating silver DC Fan 4mm Air Flo
DC Fan 4mm sq. San Ace 4 1mm thick, 15mm thick (GA type), 15mm thick, 2mm thick (GA type), 2mm thick 28mm thick (GA type), 28mm thick (GE type) 28mm thick (GV type), 28mm thick General Specifications Material
ŁaŠšfiÁ‘W“ƒDFflÅ
3. 298-21741 Weigert's Iron Hematoxylin Staining Set 1set 12,000 1 1% 96% 1 2% 0.25%, 1:1 233-01655 Weigert's Resorcin Fuchsin Solution 3,600 1g 2% 100ml 70% 400ml 2% 70% 221-01415 Van Gieson Solution
Direct Motor Drive Lead Screws / Resin Lead Screw type RM / RM RM Resin Lead Screw type RM / Resin MoBo 2 MRH 2 Features A 2-phase Stepping Motor is m
RM Resin Lead Screw typerm / Resin MoBo MRH Features A -phase Stepping Motor is mounted directly onto the shaft end of a Resin Lead Screw, which is multi-use product. Lead Screw Shaft is ideally constructed
研究成果報告書
Arterioscler Thromb Vasc Biol., 27, 2400 2406 (2007) J. Agri. Food Chem., 59, 4544 4552 (2011) Bioorg. Med. Chem. Lett., 21, 1786 1791 (2011) Advanced glycation end products (AGEs) AGEs AGEs AGEs HbA1c
Waters Sigma-Aldrich 1.0 mg/ml LC Waters Alliance HPLC ACQUITY UPLC H-Class Bio 30 cm Alliance HPLC UV ACQUITY UPLC TUV mm nm XBridge Protein B
HPLC 3. µm BEH Stephan Koza, Susan Serpa, Hua Yang, Edouard Bouvier, and Kenneth J. Fountain Waters Corporation, Milford, MA, USA HPLC 200 0 HPLC 2 2010 UPLC 200 SEC 1 UPLC SE-UPLC 2 µm BEH BEH 100% SE-UPLC
Western BLoT Immuno Booster
研究用 Western BLoT Immuno Booster 説明書 v201211 Western BLoT Immuno Booster は 抗体の反応性を増強させる成分を含む溶液で 抗体の希釈液に用いるだけで 抗原抗体反応を促進します 本製品は ウェスタンブロット ELISA 等の各種イムノアッセイに対応しており 各アッセイにおいて数倍から数十倍の検出感度向上が期待できます 西洋ワサビペルオキシダーゼ
エッペンドルフ製品価格表
エッペンドルフ製品価格表 2017 4 2 2 3 3 3 3 3 4 4 4 4 ept.i.p.s. Motion 5 ept.i.p.s. 6 ept.i.p.s. 6 ept.i.p.s. 6 ept.i.p.s. 6 ept.i.p.s. Biopur 7 ept.i.p.s. Biopur 7 ep Dualfilter T.I.P.S. 7 ept.i.p.s. LoRetention
HPLC 1M KH 2 PO 4 ph ml Anthranilic acid: AnA 600 ml ml AnA 1.0 ml / min Anthranilic acid= PRESSURE 140 kgf / cm 2
15 10-1-3. 1. HPLC 1M KH 2 PO 4 ph 3.0 50 ml Anthranilic acid: AnA 600 ml 1-1. 350 ml AnA 1.0 ml / min Anthranilic acid=137.14 PRESSURE140 kgf / cm 2 TOSOH TSK-GEL ODS- 80 Ts 1) mg / ml AnA in (φ4.6 250
Product Description The ability to isolate and study a purified protein lies at the heart of modern biochemistry. Researchers in many fields require h
Printed August 29, 2017 Version 2.0 For Research Use Only. Not for use in diagnostic procedures. DDDDK tagged Protein PURIFICATION KIT (Trial Kit) (MoAb. clone FLA-1) CODE No. 3325A PURIFICATION to Maintain
PG1501-PJ7816-CO36363-QuantStudio-3-and-5-brochure-Global-J.indd
Applied Biosystems QuantStudio 3 / QuantStudio 5 PCR PCR Applied Biosystems QuantStudio 3 QuantStudio 5 PCR Thermo Fisher Cloud 1 QuantStudio 3 and 5 Real-Time PCR Systems QuantStudio 3 and 5 Real-Time
I N S T R U M E N T A T I O N & E L E C T R I C A L E Q U I P M E N T box number basic type Standard Specification Material of Enclosure Material of F
01 Flameproof Junction Box Junction Box Series Our junction boxes are designed and manufactured based on the Recommended Practices for Explosion-Protected Electrical Installations in General Industries
steponeplus_bro_f-0912.indd
StepOnePlus /StepOne PCR PCR PCR StepOnePlus StepOne PCR PCR Features At A Glance StepOne StepOnePlus / 48 96 FAM /SYBR Green dyes VIC /JOE dyes ROX dyes 1. NED /TAMRA dyes VeriFlex Block StepOnePlus StepOne
April 15, 2004
Printed September 25, 2018 Version 3.0 For Research Use Only. Not for use in diagnostic procedures. His tagged Protein PURIFICATION KIT (MoAb. clone 2D8) CODE No. 3310 PURIFICATION to Maintain Protein
JP.qxd
Agilent 2100 1 DNA RNA Lab-on-a-Chip Agilent 2100 1999 Agilent 2100 Lab-on-a-Chip Agilent 2100 RNA DNA SDS-PAGE Agilent 2100 Agilent 2100 Agilent 2100 : (1 4 µl) 30 21 CFR Part 11 Agilent 2100 Agilent
datasheet
Printed November 11, 2015 Version 1.0 For Research Use Only. Not for use in diagnostic procedures. c-myc-tagged Protein Magnetic PURIFICATION KIT (mab. clone 1G4) CODE No. 3340 PURIFICATION to Maintain
e.jet e.jet 3 / 4 Picus eline mline 9 Proline Plus 9 Midi Plus 9 Tacta 0 9,000- Tacta MultiPacks 2 mini mini plus 4.4kg e.jet 66MP
206 e.jet e.jet 3 / 4 Picus eline mline 9 Proline Plus 9 Midi Plus 9 Tacta 0 9,000- Tacta MultiPacks 2 mini mini plus 4.4kg 206 207 3 3 207 2 2 e.jet 66MP-4 6,000 9,000 2 URL : http://support.sartorius.co.jp/campaign/
OPA277/2277/4277 (2000.1)
R OPA OPA OPA OPA OPA OPA OPA OPA OPA µ µ ± ± µ OPA ±± ±± ± µ Offset Trim Offset Trim In OPA +In -Pin DIP, SO- Output NC OPA Out A In A +In A A D Out D In D +In D Out A In A +In A A B Out B In B +In B
SDS099-B1603OB_QuantStudio-3-and-5_PB_12.indd
QuantStudio 3 / QuantStudio 5 PCR PCR Applied Biosystems QuantStudio 3 QuantStudio 5 PCR Thermo Fisher Cloud 1 QuantStudio 3 and 5 Real-Time PCR Systems QuantStudio 3 and 5 Real-Time PCR Systems 2 Applied
2
2 3 Page 4 5 6 A-1B-1 C0 D0 E0 F0 G0 A0 B0 C1 D1 E1 F1 G1 A1 B1 C2 D2 E2 F2 G2 A2 B2 C3 D3 E3 7 F3 G3 A3 B3 C4 D4 E4 F4 G4 A4 B4 C5 D5 E5 F5 G5 A5 B5 C6 D6 E6 F6 G6 A6 B6 C7 8 Page 9 1 2 3 1 2 10 1 11
1 2 3
INFORMATION FOR THE USER DRILL SELECTION CHART CARBIDE DRILLS NEXUS DRILLS DIAMOND DRILLS VP-GOLD DRILLS TDXL DRILLS EX-GOLD DRILLS V-GOLD DRILLS STEEL FRAME DRILLS HARD DRILLS V-SELECT DRILLS SPECIAL
バイオロジクス品質管理ワークフロー Integrated Protein Electrophoresis Solutions GMP 対応のための正確 迅速 簡便な分析ツール バイオロジクス ( 生物学的製剤 ) 品質管理ワークフローは GMP 規制環境下でタンパク質の純度評価に適合した製品で構成さ
Electrophoresis + Imaging + Analysis Biologics Analysis Workflow Integrated Protein Electrophoresis Solutions バイオロジクス品質管理ワークフロー バイオロジクス品質管理ワークフロー Integrated Protein Electrophoresis Solutions GMP 対応のための正確
QIAzol Lysis Reagent MaXtract High Density RNAlater RNA Stabilization Reagent QIAGEN QIAzol Lysis Reagent RNA RNase MaXtract High Density QIAzol QIAzo
Vol.7November 216 3 DNA QIAamp DNA Mini Kit QIAcube QIAamp DNA Mini Kit 2 M Lung Kidney Liver Skin and muscle Whole blood Buffy coat Lymphocytes M QIAcube DNA 48.5 kb QIAamp DNA Mini Kit (5) 5134 25, QIAamp
後期化学_01_濃度
2011 ( ) 1 4 1 100 g g % * 1 100 g 1 g 1 % * 2 (1 g)/(100 g) = 0.01 = 1 % 100 g 100 g 99 g 100 g 1 g 1 % 2.5 g 50 g (2.5 g)/(50 g) = 0.050 = 5.0 % 100 ml g 1 %(w/w) wt% % %(w/v) % / 2 % SI 2 / % 100 ml
NanoVue_H1-4.indd
NanoVue 71-2888-36 ... 2... 2... 2... 3... 4... 5... 6 NanoVue...6 NanoVue Sample... 7 Life Science... 9 1. DNA/RNA... 10 2. CyDye... 12 3.... 15 3-1Protein UV UV Protein A280 A280... 15 3-2BCABradford
01.eps
444 SumiTurn T-REX Tool Holders Cost reduction for copying with unique multiple cornered insert Carbide inserts, cermet inserts and 4 types of breakers are available. Expansion of our popular steel turning
Microsoft Word ab205921_自動染色プロトコール_YU_IS.docx
抗 PD-L1 抗体 [clone 28-8] 動免疫組織染 装置別プロトコール PD-L1 RabMAb [clone 28-8] (ab205921) BioGenex 社 i6000( オフライン抗原賦活化 ) Leica 社 BOND RX( オンライン抗原賦活化 ) Ventana 社 Ultra( オフライン抗原賦活化 ) Dako 社 Omnis BioGenex 社 i6000 プロトコール
00_1512_SLIMLINE_BOOK.indb
PIECE type SLIM type Imbalance value Less interference type, ideal for deep machining Ideal for drilling 2 PIECE REGULAR type Rigidity value Nozzle type When compared to the slim type, it has more rigidity
April 15, 2004
Printed November 21, 2017 Version 2.0 For Research Use Only. Not for use in diagnostic procedures. DDDDK-tagged Protein Magnetic PURIFICATION KIT (Trial Kit) (mab. clone FLA-1) CODE No. 3343A PURIFICATION
Purification and Properties of Acid Pyrophosphatase from the Potato Yasuko TANEMURA, Hiroshi WADA*, Takashi ITO*, Haruhito TSUGE* and Kazuji OHASHI* D
Purification and Properties of Acid Pyrophosphatase from the Potato Yasuko TANEMURA, Hiroshi WADA*, Takashi ITO*, Haruhito TSUGE* and Kazuji OHASHI* Department of Home Economics, Shotoku Gakuen Women's
AS LMD Sampling Manual
AS LMD Application Solutions LaserMicrodissection System LMD SamplingManual 2005 1 AS LMD Sampling Manual -------------------------------------------------------------2 --------------------------------------
QuantStudio_3D_Prod_Bulletin-JP-4th-0326.indd
3D PCR 3D PCR PCR 2, 3D PCR PCR 3D PCR PCR PCR PCR 1 PCR PCR Ct CNV mrna mirna GMO サンプル調製サンプルの分配 DNA の増幅結果 コピー数 / µl サンプルを多数の反応に分配します ポジティブ反応ネガティブ反応 1. PCRPCR 1 PCR PCR Ct 3D PCR PCR 3D PCR 1 /µl 2 3D
Introduction Purpose This training course demonstrates the use of the High-performance Embedded Workshop (HEW), a key tool for developing software for
Introduction Purpose This training course demonstrates the use of the High-performance Embedded Workshop (HEW), a key tool for developing software for embedded systems that use microcontrollers (MCUs)
MFS 8A3 9.8A3 #026832XE OB NO.002-21051-4 0910 NB 3,400 HOW TO USE THIS PARTS LIST 1. This Parts List contains the component parts of the Tohatsu outboard motors. 2. Please keep the Parts List updated
PCR 3 Mature mirna PCR 7 miscript mirna PCR Array C T 12 Mature mirna cdna 15 miscript mirna QC PCR Array miscript mirna PCR Array 10/2011
October 2011 miscript mirna PCR Array miscript II RT Kit miscript SYBR Green PCR Kit miscript mirna PCR Arrays miscript mirna QC PCR Array Pathway-Focused mirna PCR Array mirnome miscript mirna PCR Array