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1 SARS [ ] I. Real-time PCR B. abortus ( BA) B. melitensis (BM) B. suis (BS) B. canis (BC) Light-Cycler Real-time PCR (BCSP31) (BA OMP2ab BC OMP2ca OMP31) BA BCSP31, OMP2ab BM BCSP31, OMP2ab, OMP31 BC BCSP31, OMP2ca, OMP31 BS PCR [ ] 70 B. canis 1
2 B. canis K B. canis 16 PCR LAMP LC Y. pestis [ ] [ ] 30 K S. moniliformis 16S rrna PCR MMWR [ ] [ ] Y. pestis Y. pestis Light-Cycler Real-time PCR 10 PCR LC Y. pestis (WNV) 29 PRNT JEV WNV [ ] 2
3 RNA RNA RNA polymerase promoter RNA RNA RNA [ RV H L 1 scfv (single chain variable fragment) scfv Tomlinson I+J RV P G scfv ELISA FA scfv [ ] ] IgY RNA IgY 3
4 [ ] AAHL NiV HeV NiV HeV CVS-11 C57BL/6J ELISA ELISA [ ] CVS-11 [ ] 2 Tomlinsn I+J DNA DNA IgG scfv IgG scfv ELISA [ ] PCR DNA DNA (pgem-te) NspV self ligation PAshort-pGEM pag PCR CAPshort-pGEM cap WHO (PA5 PA8 CAP1234 CAP1301) PAshort-pGEM 331bp CAPshort-pGEM 490bp [ ] PCR (Francisella tularensis) F. tularensis subsp. tularensis(a ) subsp. holarctica (B ) PCR 16 7 ISFtu2 PCR A 2 390bp DNA B 16 5 NiV HeV BSL4 BSL4 ISFtu2 (1249p) 2 B [ ] 4
5 Francisella Short-sequence tandem repeat(sstr) (Francisella tularensis) F. tularensis [ ] (SSTR) 6 SSTR (F. tularensis subsp. holarctica) SSTR 6 2 SSTR PCR SARS SARS SARS SARS-CoV 2 hace2 hace2-tg 293T hace2 cdna pcaggsp7 DNA hace2-tg DNA PCR 3 hace2 MAbs 7 MAbs M11D3 M11H7 M13B10 M14B11 M15C6 S11E7 U22F2 MAbs M11D3 M15C6 IgM 5 IgG MAbs Francisella 11 3 MAbs M11H7 3 1 hace2-tg SARS-CoV [ ] M14B11 M15C6 F. tularensis M14B11 M15C6 4 MAbs Francisella MAbs Francisella [ ] MHC I I MHC I B I Mafa-B Mafa-I 16 Mafa-B 33 Mafa-I 14 NJ MAbs 4 IgG MAbs M13B10 M14B11 S11E7 U22F2 F. tularensis MAb M14B MAbs 20 Mafa-B 26 Mafa-I Mafa-B 1 IB [ ] MAbs 5
6 II.. GM1- chemical chaperon GM1 chemical chaperon NOEV GM1 -Gal R201C KO-Tg 0.5 mm NOEV Meg1/Grb10 Meg1,, Meg1 IGF-1 Meg1 Meg1 II ( ) GM1 GM1 Tg Tg GM1 - GM1 PCR Tg Tg -Gal KO GM1 Tg Tg Tg KO Tg botg BSE 4C30 4C30 TG B C30 ICGN 6
7 [ ] ) III ) , ,600 [ ] ) ) [ ] (TG) TG EGFP TG EGFP ) C Ascorbic acid 2-O- -Glucoside WHO WHO (WHO Alanylglutamine Collaborating Center for Defined Laboratory Animals) 7
8 [ factors that support preimplantation development of mouse ] I (1) Shiina,T., Konno,A., Oonuma,T., Kitamura,H., Imaoka,K., Takeda,N., Todokoro,K. and Morimatsu,M. Targeted disruption of MAIL, a nuclear Ikappa B protein, leads to severe atopic dermatitis-like disease. J. Biol. Chem., 279: (2) Shoji, Y., Inoue, S., Nakamichi, K., Kurane, I., Sakai, T., Morimoto, K. Generation and characterization of P gene-deficient rabies virus. Virology. 318: (3) Inoue, S., Noguchi, A., Tanabayashi, K. and Yamada, A. Preparation of a Positive Control DNA for Molecular Diagnosis of Bacillus anthracis. Jpn. J. Infect. Dis. 57: (4) Inoue, S., Asano, M., Motoi, Y., Makino, T. and Yamada, A. The Absence of Anti-Rabies Antibody in Sera of the Feral Raccoons (Procyon lotor) Captured in Hokkaido, Japan. Jpn.J.Infect.Dis. 57: (5) Iwasaki, T., Inoue, S., Tanaka, K., Sato, Y., Morikawa, S., Hayasaka, D., Moriyama, M., Ono, T., Kanai, S., Yamada, A., Kurata, T. Characterization of Oita virus 296/1972 of Rhabdoviridae isolated from a horseshoe bat bearing characteristics of both lyssavirus and vesiculovirus. Arch Virol. 149: (6) Nakamichi K., Inoue S., Takasaki T., Morimoto K. and Kurane I. Rabies Virus Stimulates Nitric Oxide Production and CXC Chemokine Ligand 10 Expression in Macrophages through Activation of Extracellular Signal-Regulated Kinases 1 and 2. J. Virol. 78: (7) Uda, A., K. Tanabayashi, O. Fujita, A. Hotta, K. Terao, and A. Yamada: Identification of the MHC class I B locus in cynomolgus monkeys. Immunogenetics 57: , (8) Okada, H., M. Ito, Y. Hirose, A. Uda, K. Terao, T. Yoshida, and T. Sankai: Buffalo rat liver cells produce embryos cultured in vitro. Comp Med 55: 61-66, (9) Okada, H., Y. Hirose, P. Manonmani, A. Uda, M. Ito, and T. Sankai: Characterization of an immortalized oviduct cell line from the cynomolgus monkey (Macaca fascicularis). J Med Primatol 34: 67-72, (10) Hara, M., T. Sata, T. Kikuchi, N. Nakajima, A. Uda, K. Fujimoto, T. Baba, and R. Mukai: Isolation and characterization of a new simian retrovirus type D subtype from monkeys at the Tsukuba Primate Center, Japan. Microbes Infect 7: , (11) Uda, A., K. Tanabayashi, Y. K. Yamada, H. Akari, Y. J. Lee, R. Mukai, K. Terao, and A. Yamada: Detection of 14 alleles derived from the MHC class I A locus in cynomolgus monkeys. Immunogenetics 56: , (12) Manonmani, P., H. Okada, N. Ogonuki, A. Uda, A. Ogura, T. Yoshida, and T. Sankai: Fertilization and preimplantation development of mouse oocytes after prolonged incubation with caffeine. Reprod Med Biol 3: , (13) Hotta, A., G. Q. Zhang, M. Andoh, T. Yamaguchi, H. Fukushi, and K. Hirai: Use of monoclonal antibodies for analyses of Coxiella burnetii major antigens. J. Vet. Med. Sci. 66: , (14) Noguchi A, Takekawa N, Einarsdottir T, Koura M, Noguchi Y, Takano K, Yamamoto Y, Matsuda J, Suzuki O. Chromosomal mapping and zygosity check of transgenes based on flanking genome sequences determined by genomic walking. Exp Anim Apr;53(2): (15) Yamaguchi-Yamada M, Manabe N, Uchio-Yamada K, Akashi N, Goto Y, Miyamoto Y, Nagao M, Yamamoto Y, Ogura A, Miyamoto H. Anemia with chronic renal disorder and disrupted metabolism of erythropoietin in ICR-derived glomerulonephritis (ICGN) mice. J Vet Med Sci Apr;66(4): (16) Koura M, Handa H, Noguchi Y, Takano K, Yamamoto Y, Matsuda J, Suzuki O. Sequence analysis of cdna encoding follicle-stimulating hormone and luteinizing hormone beta-subunits in the Mongolian gerbil (Meriones unguiculatus). Gen Comp Endocrinol May 8
9 1;136(3): (17) Takano K, Koura M, Noguchi Y, Yamamoto Y, Uchio-Yamada K, Matsuda J, Suzuki O. Sequence analysis of cdna encoding follicle-stimulating hormone and luteinizing hormone beta-subunits in the Mastomys (Praomys coucha). Gen Comp Endocrinol Sep 15;138(3): (18) Masujin K, Okada T, Tsuji T, Ishii Y, Takano K, Matsuda J, Ogura A, Kunieda T. A Mutation in the Serum and Glucocorticoid-Inducible Kinase-Like Kinase (Sgkl) Gene is Associated with Defective Hair Growth in Mice. DNA Research, 11: , (19) Kamei Y, Miura S, Suzuki M, Kai Y, Mizukami J, Taniguchi T, Mochida K, Hata T, Matsuda J, Aburatani H, Nishino I, Ezaki O. Skeletal muscle FOXO1 (FKHR)-transgenic mice have less skeletal muscle mass, down-regulated type I (slow twitch / red muscle) fiber genes, and impaired glycemic control. J Biol Chem, 279: , (20) Uchio-Yamada K, Manabe N, Goto Y, Anann S, Yamamoto Y, Takano K, Ogura A, Matsuda J. Decreased expression of matrix metalloproteinases and tissue inhibitors of metalloproteinase in the kidneys of hereditary nephrotic (ICGN) mice. J Vet Med Sci. 67:35-41, in SA Medicine, 6, 14-18, , , Zoonosis 55, 2-4, , , Medical Corner, 115, 14-17, , 17-22, pp , in pp , ,,, Q&A in Q&A pp , in, pp.85-90, in, pp , in, pp ,
10 26 in, Society for Cell Biology, Washington, DC, USA, December 4-8, pp , World Focus No ) : SARS , 2004.,,, Brucella canis ,,, PCR DNA MHC class I PCR (Francisella tularensis) ,,, Yersinia II. (1) Inoue,S., Noguchi, A., and Akio,Y. Preparation of Safe and Reproducible Positive-control for Moleclar Diagnosis of Rabies. Fortieth anniversary United States-Japan Cooperative Medical Science Program (38th Joint Working Conference on Viral Diseases). 7-9 December, Kyoto, Japan. (2) Suzuki O, Hata T, Takekawa N, Koura M, Takano K, Yamamoto Y, Noguchi Y, Uchio-Yamada K, Matsuda J, In Vitro Growth Culture Of Preantral Follicles From Mouse Ovaries Cryopreserved Using Vitrification, 37th Annual Meeting of the Society for the Study of Reproduction, Vancouver, BC, Canada, August 1-4, (3) O. Suzuki, M. Koura, Y. Noguchi, K. Takano, K. Uchio-Yamada, Y. Yamamoto, J. Matsuda, Characterization of the Follicle Stimulating Hormone β-subunit Precursor Protein cdna in the Rabbit, 44th Annual Meeting of the American pestis PCR, Real-time PCR (Light-cycler:LC), LAMP CVS-11 C57BL/6J DNA PCR (Francisella tularensis)
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