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4 910 (MHC) (MHC) Swine Leukocyte Antigen(SLA) SLA MHC SLA (Hp-1a.1 ) SLA (2.4Mb) ) SLA 27 SLA 151 ( 1) 2) UBD ORF43-3 ORF43-2 ORF-1 GABBR1 MOG Zfp57 C7H6orf12 ZNRD1 PPP1R11 RNF39 TRIM31 TRIM40 TRM10 TRM15 TRIM26 AFP RAP1A SLA-1 SLA-5 SLA-9 SLA-3 SLA-2 SLA-4 SLA-11 TRIM39 RPP21 GNL1 PRR3 ABCF1 PPP1R10 MRPS18B C7H6orf134 C7H6orf136 DHX16 AB NRM MDC1 TUBB FLOT1 IER3 AB DDR1 GTF2H4 VARS2L AB DPCR1 C7H6orf205 C7H6orf15 AB PSORS1C1 PSORS1C2 CCHCR1 TCF19 POU5F1 MIC2 MIC1 SLA-8 SLA-7 SLA-6 MCCD1 BAT1 ATP6V1G2 NFKBIL1 LTA TNF LTB BX LST1 NCR3 AIF1 BAT2 BAT3 APOM C7H6orf47 BAT4 CNK2B LY6G5B LY6G5C BAT5 LY6G6E LY6G6D LY6G6C C7H6orf25 DDAH2 CLIC1 MSH5 C7H6orf26 C7H6orf27 VARS2 LSM2 HSPA1L HSPA1A HSPA1B AL NEU1 C7H6orf2 BAT8 ZBTB12 C2 BF RDBP SKIV2L DOM3Z STK19 C4A CYP21A2 TNXB CREBL1 FKBPL C7H6orf31 PPT2 EGFL8 AGPAT1 RNF5 AGER PBX2 GPSM3 NOTCH4 BTNL5 BTNL6 BTNL4 BTNL3 BTNL2 DRA DRB4 DRB3 DRB2 DRB1 DQA DQB2 DQB1 DOB2 BX BX DRB5 DYB DOB1 TAP2 PSMB8 TAP1 PSMB9 DMB DMA BRD2 DOA COL11A2 RXRB SLC39A7 HSD17B8 RING1 1 SLA SLA SLA I II 6 20 SLA 200

5 6 ISAG SLA Nomenclature Committee MHC ( ac.uk /ipd/mhc/sla/index.html SLA SLA cdna SLA-DNA I II : Hp- () SLA-1 SLA-2 SLA-3 DRB1 DQB1 Clawn (c1) *0401 *w09an02 *0602 *11ac21 * (c2) null? *06an03 *03an02 *0801 * (c3) *0401 *w09an02 *0602 *0801 * (c4) null? *06an03 *03an02 *11ac21 *0601 Duroc (d1) *08an03, *06an04 *0102 *0101 *1101 * (d2) *-an02 *1002 *0502 *0403 *0303 Large White 1.1 (H01) *0101 *0101 *0101 *0101 *0101 NIH (MGH) 2.2 (a) *0201, *0701 *0201 null *0201 * (c) null *0301 *0301 *0301 *0301 4a.4 (d) *0401 *0401 *0401 *0201 * Yucatan 4b.5(x) *0401 * *0401 *0501 * (w) *0401 *w08sw01 *05sw01 *0501 * (y) *08sy01 *05sy01 *0601 *0601 * (z) *0801 *0502 *0701 *0801 *0202 Westran 8.9 *02we02, *04we01 *07we01 *0302 *0201 *0402we01 Sinclair 9.10 (a) *0601 *0601 *0501 *0401 * (c) *0501 *0302 *hm22 *0901 * (d) *0101, *w09sm09 *0501 *0701sm19 *0602 *0701 Hanford (e) *08sm08, *w09sm09 *10sm01 *0701sm19 *0403 * (f) *w10sm21 *w13sm20 *0401 NT NT NIH SLA 2 ( 3) )( 1) SLA ()SLA SLA SLA SLA MHC SLA SLA SLA SLA SLA Renard C., et al.genomics 88, , Ando A., et al. Immunogenetics 60, , Soe OK., et al. Xenotransplantation, In press.

6 910 () (3873%) 26% 514% () (2001) () (0.6)

7 (2005)95% 88% 79% 70% 45% () () 0.5 ml 100% (2005) Tominaga K., Hamada Y., Hochi S.Gel-loading tip vitrification of in vitro-matured bovine oocytes and subsequent embryo production by IVF and nuclear transfer. J.Mamm.Ova Res.22, , Kuwayama M.Oocyte cryopreservation.( Mini Review) J.Mamm.Ova Res.24,2-7, 2007.

8 1011 UASB MAP UASB () UASB MAP UASB UASB COD 21,600mg-COD/lSS 12,800mg/l kg-cod m -3 d -1 (HRT 5 ) COD 85% VFA 250mg-COD/l 70 MAP Mg/P 1.3 ph89 MAP ph10 20% EDX P Mg Ca X Removal(%) UASB PO4-P PO4-P(SS) NH4-N NH4-N(SS) ph MAP MAP (HAP)HAP MAP SS

9 UASB SS UASB SS UASB UASB MAP MAP SS SS (Uemura S., Harada H.:Production of biologically safe digested manure for land application by a full-scale biogas plant with heat-inactivation. Waste Management and Research, 26, , UASB SS vol.43, No.11, , Uemura S., Harada H.Treatment of wastewater containing high amount of suspended solids by a novel multi-staged UASB reactor. Environmental Technology, vol.26, , 2005.

10 ( ) (COC) (MK) MK(bMK)cDNA bmk bmk cdna bmk( 16kDa) MK () bmk bmk bmk(200ng/ml) COC (:50% 21%P<0.05COC (DO) bmk bmk DO bmk (CMMK+CMMK-)DO DO CMMK+ (100) bmk COC bmk Tunnel LM-PCR 2) bmk

11 30 10 bmk bmk bmk bmk 5 bmk COC DO bmk DO COC bmk Ikeda S., et al.mol. Reprod. Dev., 57: , Ikeda S., et al.reproduction, 132: , 2006.

12 % 30 5 (1916 ) (1998 )() 1.6 ha 618 ha 1.6 ha 63.6 () () () Y = X (Y=gDM/m 2 ; X=; r=0.9, p<0.001) :133gDM/m CP 11.9% ((2004)) 1314ha/() CP 56 CP (CF) () ((2005)(2005))

13 a b b ab b b ha ha 208DM 300ha 945DM DM 4% 2005 ( )() 55(2): (3): (1):

14 (VFA) VFA VFA VFA (VFA ) VFA VFA ( 1) VFA (1991) ()(1994) VFA VFA VFA

15 VFA VFA VFA VFA VFA (LG)(Profec) VFA IgA VFA LG Profec K. Kitade, et al.effects of weaning on carbonic anhydrase activity in the palotid gland, rumen and abomasum of Holstein calves. Journal of Comparative Physiology B, 172, , S. Yonekura, et al.effects of aging and weaning on mrna expression of leptin and CCK receptors in the calf rumen and abomasum. Domestic Animal Endocrinology, 22, 25-35, 2002.

16 1214 () () ()

17 (2003 ) 103 (2004 ) (2004 )Best Poster Award, International symposium on Microbial Ecology, Asia 2007 Otawa K., Asano R., Nakai Y., et al.molecular analysis of ammonia-oxidizing bacteria community in intermittent aeration sequencing batch reactors used for animal wastewater treatment. Environ. Microbiol., 8(11), , Sasaki H., Yano H., Sasaki T., Nakai Y. A survey of ammonia-assimilating micro-organisms in cattle manure composting. J. Appl. Microbiol., 99, , 2005.

18 F2 () () A B S-SAP 2 ()

19 A B () () S-SAP IAP ()

20 1415 () 100 () (delipation) delipation() delipation delipation a b c 1 delipation() (a)(b)(c)

21 delipation a b 2 (a)4-8 (b) Ushijima H., Yoshioka H., Nagashima H., et al.improved survival of vitrified in vivo-derived porcine embryos. Journal of Reproduction and Development, 50, , Esaki R., Ueda H., Nagashima H.,et al.cryopreservation of porcine embryos derived from in vitro matured oocytes. Biology of Reproduction, 71, , Nagashima H., et al.production of Live Piglets Following Cryopreservation of Embryos Derived from In Vitro-Matured Oocytes. Biology of Reproduction, 76, , 2007.

22 () () () 14 12% (TDN ) TDN TDN TDN TDN=0.91 OCW (OCW+NCWFE) (OCWNCWFEOCW OCW ) 105 TDN 11.6~38.4% 4.0~25.2%TDN 23.1~106.1% E

23 () 1 A B C () 20% 30% 10% 5-10% (30-35%) % TDN TDN (kg/) % 10~20% ( 22.6 /) TDN

24 1718 -(BLG) () BLG (5 ) BLG (AB ) BLG - A B AB ) (FDAFood and Drug Administration) () (EFSAEurope Food Safety Authority) ( 20 )

25 ) () (6 ) Watanabe S., T. NagaiJ Reprod Dev, 54,6-17, Yamaguchi M., M. Itoh, Y. Ito, S. WatanabeJ Reprod Dev, 54, , Watanabe S., T. NagaiAnim Sci J, 2009.(in press)

26 ( 1(a))( 1(b)) ( 1(c))( 1(d)) (a)(b)(c) (d) () 5864 ( 1)

27 (F10 14 /mols -1 ) F 1.0 F 1.0 F 0.8 F 0.8 F 0.5 F 0.5 / 21/ / / / / / (Hanwoo)

28 1920 SPR PCR (SPR PCR ) (BmN4 )(Sf-9 SF+ ) Micrococcus lysodeikticus (SF+)() ( 1) () SF mOsm/kg (321mOsm/kg) 296mOsm/kg 1.5 () (/L)

29 SF+ (220309mOsm/kg) 2 () Tsuchiya, et al.comparison of artificial synthesis method of gene, Nucleic Acids Symposium Series, 50, ,

30 1920 (AKAV) 1984 Iriki 2006 Iriki AKAV AKAV(Okayama2001 Okayama2004) AKAV AKAV AKAV Okayama2001 Okayama2004 AKAV RNA ( 1) Okayama2004 OBE-1 Okayama2001 Iriki NS-88-1 ON-89-2 NS Okayama KC-04Y JaGAr39 FO NBE-9 SAB' M OBE-1 KT3377 KC-12X84 KC-15X84 Iriki PT-17 KM-29X MZ-90-1 MZ NT-14 Okayama2001 YG-88-2 FO-90-4 KS-90-2 KS-90-3 ISR-01 R7949 B8935 I IIa IIb III AKAV

31 OBE-1 LMS RNA ( 2) cdna L RNA DNA ( )I S (NSs) T7 RNA Gn Gc N S RNA L RNA M RNA N N AKAV L M S cdna cdna cdna AKAV AKAV T7 RNA mrna N LRNA T7 RNA AKAV (RNP) ( 3) OBE-1 Iriki cdna OBE-1 Ogawa Y., Sugiura K., Kato K., Tohya Y. and Akashi H.Rescue of Akabane virus (Bunyaviridae) entirely from cloned cdnas using RNA polymerase I. J. Gen. Virol., 88, , Ogawa Y., Fukutomi T., Sugiura K., Sugiura K., Kato, K., Tohya Y. and Akashi H Comparison of Akabane virus isolated from sentinel cattle in Japan. Vet. Microbiol., 124, 16-24, 2007.

32 1920 TMR(Total Mixed Ration) 1 1 1, TMR 54 Milking Status Milking Status ( 11,300kg) 4 ( 50)( 1)

33 Milking Status 5 Senosy Waleed, Takeshi Osawa, Yoshiaki Izaike.Theriogenology, submitting. I.Martin Sheldon, et al.thriogenology, 65, , 2006.

34

35 (03) FAX (03)

36 New Technology for Livestock

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