Wako
|
|
|
- みさき うるしはた
- 4 years ago
- Views:
Transcription
1 Wako
2 () C-B ( M) 1, 3-Fucosyltransferase V, Human Recombinant 50 munits 45,600 Gal1, 4 GlcNc 1, 3-FucT Gal1, 4 (Fuc1, 3) GlcNc GDP-Fucose GDP-Fucose GDP 2 mg 50, C-B ( M) 1, 4-Galactosyltransferase, Bovine, Recombinant 1 unit 22,900 GlcNc 1, 4-GaIT Gal1, 4GlcNc GDP-Galactose GDP SIG (U4500) UDP-Galactose 5 mg 25 mg 10,000 32, C-B ( M) 1, 2-Mannosyltransferase, Catalytic Domain, Saccharomyces cerevisiae, Recombinant 100 munits 34,200 Man1, 2Man-Thr (Ser) -Protein 1, 2-ManT Man1,2 Man1, 2Man-Thr (Ser) -Protein GDP-Mannose GDP SIG (G5131) SIG (G7377) GDP-Mannose (Yeast) 50 mg 32,800 GDP-Mannose (Synthetic) 5 mg 7, C-B ( M) 2, 3- (N) -Sialyltransferase, Rat, Recombinant 100 munits 45,600 Gal1, 3 (4) GlcNc 2, 3-ST Neuc2,3Gal1,3 (4) GlcNc CMP-Neuc CMP CMP-Neuc 10 mg 16, C-B 2, 6-Sialyltransferase, Rat Liver 100 munits 28,600 ( M) , 6-Sialyltransferase, Rat Liver 50l 17,000 Gal1, 4 GlcNc 2, 6-ST Neuc2,6Gal1,4GlcNc CMP-Neuc CMP CMP-Neuc 10 mg 16,800
3 N- N N N TOR (B6825) 30,000 10mg 10mg 10mg 10mg 10mg 10mg 10mg 25mg 30,000 10,000 20,000 25,000 30,000 30,000 18, mg 19, mg 15, mg 38, TOR 100mg 23,100 (C6660) C-B 25mg ( M) TOR 10mg 34,500 (D2360) C-B 5mg ( M) mg 16,500 N mg 50,000 N
4 1) Suzuki, S. S. et al. : J. Cell. Biochem., 51, 181 (1993) 2) Seftor, R. E. B. et al. : Melanoma Res., 1, 43 (1991) 3) J. Biol. Chem., 261, 4766 (1986) 4) Eur. J. Biochem., 150, 41 (1985) 1) Myers, R. W. et al. : nal. Biochem., 101, 166 (1980) 2) Potier, M. et al. : nal. Biochem., 94, 287 (1979) SIG (U4375) SIG (U5252) UDP-Glucose2Na UDP-N-cetyl Glucosamine2Na UDP-N-cetylglucosamineNa UDP-N-cetylgalactosamine2Na UDP-Glucuronic cid3na 250 mg 1 g 100 mg 25 mg 100 mg 5 mg 100 mg 500 mg 5,000 17,000 9,000 4,900 12,700 14,100 7,000 28,000
5 l l l l l l l l l l l
6 PI PIP2 PLCIP3DG CaCaMKPKC PIIP3 Ca 2 IP3C PBS (0.5mgml) No.IPR.1 IgG2b IP3C , ,000 l PI3p85 No.U5 IgG1 PI3p () l PI3p85 No.T4 IgG1 PI3p85 11,000 () llllllllllllllllllllllllllllllllllll
7 80 () --D- (-) HS-J EUml 1. LL HS-T 0.2ml25 2. Control Standard Endotoxin 500ng (LPS) r = l ( EUml) (0.03EUml) sssssssssssssssssssssssssssssssssssss LED () () SLP ,900,000 1,800, , ,000
8 & ll ll
9 l (Cryptosporidium parvum, C. muris, C, baileyi) ( ) // () ml (Cryptosporidium parvum) ()FITC 2.7ml ( 10) () 50ml ()10 100ml
10 () () (IL-1, IL-4, IL-6, IL-10) () () () HRP (100) l l 30ml 7ml12ml 0.15ml () HRP 15ml () (TMBz) 12ml () (25) 50ml () 12ml (10) l l l IL-1IL-1IL-1IL-3IL-5IL-6IL-7 IL-8IL-10IL-12IL-13GM-CSFIFN-IL-2IL-6IL-10IFN-TNF-TNF- MIP-2MCP-1 l l l IL-4IL-1IL-2IL-3IL-4IL-5IL-7IL-8IL-10IL-12IL-15IL- 2IL-3IL-4IL-6IFN-TNF-MCP-1MIP-2TNF-
11 l l l IL-6IL-1IL-2IL-6IL-7IL-8IL-13IL-15IFN- IL-1IL-2IL-10MCP-1TNF-IL-1IL-4IL-10TNF-MIP-2 l l l IL-10IL-1IL-7IL-8IL-10IL-15IFN-GM-CSFRNTESTNF- IL-1IL-2IL-3IL-4IL-6IL-10TNF-IL-1IL-4TNF-IFN-MIP-2 Wako Wako Wako
12 Group GroupGroup GroupGroup Group GroupmGluR2mGluR4 100 mg 6,500 5 mg 26,500 5 mg 26,500 5 mg 19,500 5 mg 19, mg 54, mg 91, mg 54, NMD 5 mg 40,000 NMD mg 54,000 TOCRIS COOKSON 1997FX WKO BIO WINDOWFX
13 (PTN) (MK) 1) PTN136 MK 2) 50% PTN 1) 1) 3) 4) l PTNMK MK PTNPTNMK PTNPTNMK PTNMK 1) Science, 250, (1990) 2) J. Pept. Sci., 2, (1996) 3) Biochem. Biophys. Res. Commun., 173, (1990) 4) Proc. Natl. cad. Sci. US, 93, (1996) v 4298-v 4299-s Pleiotrophin (Human) Midkine (Human) Midkine (Human, ) 50g 50g 0.1 mg 30,000 30,000 30,000
14 l l l l l l l BK021 BK022 BK001 BK002 BK003 BK004 BK005R BK005F BK006 BK007F BK007R BK008 BK009 BK010 BK012 BK014 BK015 BK016 BK017 BK018 BK020 (Muscle ctin) (Non-muscle ctin) (Rhodamine based Kit) (Fluorescein based Kit) (Fluorescein based Kit) (Rhodamine based Kit)
15 l l l l l l l l l l l l () 95 V V V V ccccccccccccc 80 (1-2-) 14 (2-) V V V 95 V 18l 18l 9,000 9, l 18l 9l 18l 5,500 9,800 6,900 10,500
16 M (EthD-1) M (em530nm) EthD-1 (em>600nm) Cr M40l [4mM DMSO] 150l [2mM DMSO/H2O 1 : 4 (v/v )] 1,000 1, SYTO10 (em510nm) DED Red (em600nm) 24 SYTO1050l [DMSO]1 DED Red50l [DMSO (20%) ]
![NKLKT DiOC18 (ex : 484nm,em : 501nm) Propidium iodide (ex : 536nm,em : 617nm) 51 Cr DiOC18200l [DMSO]1Propidium iodide1.1ml [3.](/docs-images/105/167298585/images/17-0.jpg "75mM ]1 2,000 200 () (10l) () 0.15610.0ngml 1. 961 2. 200ngml 20l 3. 60ml 4. 5l 5. - 10l 6. OPD 2 7. 12ml 8. (1moll H2SO4) 12ml 9.")
17 NKLKT DiOC18 (ex : 484nm,em : 501nm) Propidium iodide (ex : 536nm,em : 617nm) 51 Cr DiOC18200l [DMSO]1Propidium iodide1.1ml [3.75mM ]1 2, () (10l) () ngml ngml 20l 3. 60ml 4. 5l l 6. OPD ml 8. (1moll H2SO4) 12ml 9. (10) 50ml
18 ISOHIR () DN 1DN DN 1cm6cmDN 1/4p53 (exon11)seminested PCR (first PCR1296bpsecond PCR 265bp) MCT VNTR (variable number of tandem repeat) BC 36cm DN1/4 MCT118 PCR DN 15cm PCR DN T4 gene 32 proteinpcr PCR (lane 1)DN () DN1/20 (lane 2)T4 gene 32 protein (lane 3)DN DN (280bp) PCR (lane 4) T4 gene 32 protein (lane 5) T4 gene 32 proteint4 gene 32 proteinpcr T4 gene 32 protein
19 1mm 0.5mg1mg 2.5mg5mgDN1/10 DN (D 279bpp53 (exon10 279bp) PCR p53 TPduplex DN duplex DN, blant duplex DN, nicked duplex DNRN-DN hybrid partial DN 620,000unitsmg (TPase ) 97 (SDS-PGE) 200lml 76kDa uvrde. coli 20l371nmoleTP 1unit40ng helicase92bpduplex DN50 20mM Tris-HCl (ph8.3) 200mM NaCl25mM 2- mercaptoethanol1mm EDT0.5mM EGT 50 Glycerol 80 20l25mM Tris-HCl (ph7.5) 20mM NaCl3mM MgCl22mM 2-mercaptoethanolmM TP2M 92bp partial duplex DN helicase3710 J. Biol. Chem., 262, 2066 (1987) M ) Matson, S. W. and George, J. W. : J. Biol. Chem., 262, 2066 (1987) 2) Runyon, G. T. and Lohman, T. M. : J. Biol. Chem., 264, (1987) 3) Runyon, G. T. and Bear, D. G. et al. : Proc. Natl. cad. Sci. US, 4) Matson, S. W. : Proc. Natl. cad. US, 86, 4430 (1989) 5) Geoprge, J. W., Brosh, R. M. et al. : J. Mol. Biol., 235, 424 (1994) 6) Rumyon, G. T., Wong, I. and Lohman, T. M. : Biochemistry, 32, 602 (1993) 87, 6383 (1990)
20 1) (P ) 2) 3) 10 4 P DN (a) (b)dndn (P) (c)p PP DNDN (d) DN (e) RPP (f)pod (g)p
21 ELIS 1 ) (DN 96 12) 1 2 ) 2 3 ) 100ml 4 ) 3 12ml 5 )HRP (400) 0.1ml 6 ) 12ml 7 ) 12ml 8 ) (20) 100ml 9 ) (2.5N) 12ml 10) 4 11) DN 3 (H3) - -HRPHRP ttttttttttttttttttttttttttttttttttttttttttttttt IL-3TF-1 IL-372 DN bcl-2elis (I25) bcl-2 ELIS (I23) DN IL-324 DN (1) bcl-2 ( 2) I25 Nucleosome ELIS 96 45,600
22
23 B D E C G H F H FX a,b,c,d,,,,fx 10 (3,000) a b c d 1031 FX XLM04256niftyserve.or.jp No.7 "" () sssssssssssssssssssssssssssssssssssssssssssssssssss
24 WKO BIO WINDOW No.8 OCT MediaKapMediaKap PlusPlus ME2M02018S 0.2m MediaKap l 18 17, ME2M05018S 0.2m MediaKap 5 25l 18 36, ME2M10018S 0.2m MediaKap l 1/4 () 18 56, ME2M02B12S 0.2m MediaKap l 12 14, ME2M05B12S 0.2m MediaKap 5 25l 12 27, ME2M10B12S 0.2m MediaKap l 1/4 () 12 40, ME2M25B06S 0.2m MediaKap l 1/4 () 6 31, ME2M50B03S 0.2m MediaKap l 1/4 () 3 22, MP2M02018S 0.2m MediaKap 2 Plus 0.22l 18 28, MP2M05018S 0.2m MediaKap 5 Plus 25l 18 45, MP2M02B12S 0.2m MediaKap 2 Plus 0.22l 12 20, MP2M05B12S 0.2m MediaKap 5 Plus 25l 12 33, MP2M10B12S 0.2m MediaKap 10 Plus 510l 1/4 () 12 50, MP2M25B06S 0.2m MediaKap 25 Plus 1025l 1/4 () 6 38, MP2M50B03S 0.2m MediaKap 50 Plus 2550l 1/4 () 3 26,000
Bio-No.57/3....
2004. MAR. 3No.57 http://www.wako-chem.co.jp C O N T E N T S 2 Wako Bio Window No.57 MAR.2004 l l l l wako wako l l mmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmm Wako Bio Window No.57 MAR.2004 3 4 Wako Bio Window
10中西_他.indd
Streptomyces mobaraensis N N N Sm b Sm N N Sm Sm Sm ' ' Sm Sm ak K k K bk k K Sm Sm a b S. mobaraensis a b a b Sm b b bb b b Sm Sm S. lividans ab Sm ab Sm ab ab ab ab SmSm S. lividansab S. lividans ab
能書単頁9[1].5(2)
![能書単頁9[1].5(2)](/thumbs/87/96620151.jpg "能書単頁9[1].5(2)")
ips CytoTune -ips ver.1.0 1 I CytoTune ips 3 II 3 3 4 5 III CytoTune ips ips 6 6 6 6 7 1) 2) ips 8 ips 8 SeV 9 SeV 10 IV Q&A 11 V 12 12 VI...13 13...13 2 I CytoTune ips CytoTune ips 4 OCT3/4SOX2 KLF4c
ÿþ
インターロイキン インターロイキンは リンパ球や単球 マクロファージなどの免疫反応への寄与が知られる白血球から産生されることから 細胞間 ( inter-) 白血球 ( leukocyte -leukin) より Interleukin と名づけられました 現在までに 30 種類以上のインターロイキンが同定され 発見された順番に番号が付与されており 中にはケモカイン ( IL-8) やインターフェロン
後期化学_01_濃度
2011 ( ) 1 4 1 100 g g % * 1 100 g 1 g 1 % * 2 (1 g)/(100 g) = 0.01 = 1 % 100 g 100 g 99 g 100 g 1 g 1 % 2.5 g 50 g (2.5 g)/(50 g) = 0.050 = 5.0 % 100 ml g 1 %(w/w) wt% % %(w/v) % / 2 % SI 2 / % 100 ml
Yeast Two-Hybrid System Kit N-G Wako & TranSort
Yeast Two-Hybrid System Kit N-G Wako & TranSort l (small unilamellar vesicle : SUV) DNA l l l l l l l 12ml35mm 110 5 21824 (4080 ) 37CO2 (5) 3 DNA0.252g50l DNA 440l2.520l 3.DNA 15DNA- l l l l l l 522mlPBS1.9ml
2007050781......_.L.O...z.W
a s d f g a s d f g h j k l 0 in vitro in vivo in vitro in vivo in vivo in vivo in vitro Metabolism Metabolism Endocr Endocr Endocrinol Ann NYA cad Sci J clin Endocr Metab
1) van Meer, G., Op den Kamp, J. A.: J. Cell. Biochem., 19, 193-2040982) 2) Gaffet, P., Bettache, N., Bienvenue, A.: Biochemistry, 34, 6762-6769(1995) 3) Sims, P. J., Wiedmer, T.: Thromb. Haemost., 86,
表1.eps
Vol.1 C ontents 1 2 cell-free EMBO J Proc. Natl. Acad. Sci. USA NatureEMBO J 3 RNA NatureEMBO J Nature EMBO J 4 5 HCV HCV HCV HCV RNA HCV in situ 6 7 8 Nat Struct Mol Biol J Biol Chem Nat Commun J Virol
細胞増殖測定細胞染色プロトコル
Cell Counting Kit Cell Counting Kit -8 3 Cell Counting Kit -F -Cellstain- -Cellstain- -Cellstain- Calcein-AM solution -Cellstain- CytoRed solution -Cellstain- BCECF-AM special packaging -Cellstain- -Cellstain-
TECN
WKO BIO WINDOW lyo in situ wako No.13 UG. 1998 TECN TECN l l l l l l l hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh lyo lyo l J.Clin. Biochem. Nutr., Mebio hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh
tary adenylate cyclase activating polypeptide ; PA-
Conformation of a peptide ligand bound to its G-protein coupled receptor and its implication for ligand transportation tary adenylate cyclase activating polypeptide ; PA- molecular biology (ed. Rich,
Biology of spermatogenesis and spermatozoa in mammals. Biophys. Biochem. Cytol. Arch. Biochem. Biophys. Anat. Rec. Biol. Reprod. Mol. Hum. Reprod. Biol. Reprod. Reprod. Biol. Endocrinol. Aust. J. Zool.
Microsoft Word - 高橋＿国試講習会2006m.doc
2006 19 150 20 200 19 80 2 70 1 45 1 1 30 20 105 2 40 1 1 31 95 2 25 1 1 32 1. 2. 3. 4. 5. 1 [] 25 12.5% 2006/3 6 2 1 2 3 7 A a b c d 11 e B a b c d C a b c D a b E a b c d 2 15 16 17 18 19 20 2001 2002
19 8 1970 20 10 4 1
19 8 1970 20 10 4 1 IgE 1960 % 1970 20%, 1990 40% 1998 70% 20 IgE 21 90% IgE IgE (%) 70 60 50 40 30 20 10 0 1970 1975 1980 1985 1990 1995 2000 2005 1. 2005 10 2 30 IgE 2. 3 1. B IgE T IgE IgE IgE 2 T IgE
無印良品 2012 自転車 カタログ
26 897895321,000 140cm 76.0cm 16.0kg H LED 3 263 897896025,000 140cm 76.0cm 16.5kg H 3 LED 20 8978984 8978977 19,800 134cm 73.0cm 15.0kg LED 2620 2620 8486656550 5536207483 14512372,100 8279999840 26 77342561,417
2
1999 No. 5 CHAPERONE NEWSLETTER CONTENTS 2 3 4 JEM JEM JEM, SEC, SCJ JEM JEM SCJ jem, scj 5 in vitro S. cerevisiae 6 K 7 in vitro Cell Mol. Cell in vitro 8 9 E. coli E. coli 10 in vitro ssc - b 11 in vivo
2 (1) (2) SCI 2 SCI 2 24 2 12 2
2004 (1) (2) (2) (3) (1) 1 (2) (3) 2 (1) (2) SCI 2 SCI 2 24 2 12 2 100% / 16 2002 2003 http://lin.lin.go.jp/alic/month/dome/1997/nov/chousa.htm (05 612 1315 1618 1922 2329 3039 4049 5059 60 ) =10 b g 1
Short Review Database Center for Life Science Online Service Shou Takashima, Masafumi Tsujimoto, Shuichi Tsuji, Fun
Short Review Shou Takashima, Masafumi Tsujimoto, E-mail : staka@postman.riken.go.jp Shuichi Tsuji, Functional analysis of sialylglycoconjugates by sialyltransferase deficient mice GINAcT : N-acetylglucosaminyltransferase,
The function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H) Database Center for Life Science Online Service Key words Shigey
The function and structure of NAD kinase: the key enzyme for biosynthesis of NADP (H) Key words Shigeyuki Kawai, Chikako Fukuda, Kousaku Murata E-mail : kawais@kais.kyoto-u.ac.jp, chikakko@kais.kyotou.ac.jp,
gtf Wnt5a-Ror2 IL-8 in vitro Ozony Tron P. gingivalis SOD ELISA Location of main occluding areas and masticatory ability in patients with reduced occlusal support 12-Methacryloyloxydodecylpyridinium bromide
Novel chemical mediators in the resolution of inflammation Charles N. Serhan Key words E-mail : marita@nih.go.jp Charles N. Serhan Brigham & Women's Hospital/Harvard Medical School 1) Serhan, C. N.,
untitled
E-mail: khatano@fms.saitama-u.ac.jp Tel & Fax: 048-858-3535 Toxin Virus Bacterium Glycolipid Glycoprotein Vero toxin (Stx1 and Stx2) Side view Bottom view H H H H N H Grobotriaosyl Ceramide (Gb 3 : Galα1-4Galβ1-4Glcβ-Cer)
Correlation between molecular evolution of P450s and evolution of plant secondary metabolism Masaharu Mizutani E-mail : mizutani@scl.kyoto-u.ac.jp URL : http://biofun.kuicr.kyoto-u.ac.jp/index-j.html 13)
2
1 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234 12123456789012345678901234
A, B 2 B 5 A C A ADP A G ADP ADP G camp B GM A B CTB Tochikubo et al., 1998 CTB CTB Dertzbaugh & Elson, 1993a; Yu et al., 1994; Shi et al., 1995 CTB C
2004 Analysis of the expression of cholera toxin B-subunit gene 1065010 2004 8 26 1 A, B 2 B 5 A C A ADP A G ADP ADP G camp B GM A B CTB Tochikubo et al., 1998 CTB CTB Dertzbaugh & Elson, 1993a; Yu et
Taro9-医薬品目次２０１２－１.PDF
5 0.1% 11 250mg 10 20mg 10 5ml 19 0.05% 13 1.25mg 10 1%5ml 19 10mg 10 0.2%100ml 18 25%50ml 9 2mg 17 5%250ml 9 20%25g 20 500 g 11 4 10g 20 Cap150mg 1 200mg 0 Cap90mg 5 20mg 10 10% 5 18 10mg 10 3%30g 20
2007 Vol.56 No.6 総説 丸山浩樹
Reprinted from RADIOISOTOPES, Vol.56,.6 June 2007 Japan Radioisotope Association http : //www.jrias.or.jp/ α β α β ε m z α µ µ α m z m z µ µ Curr. Opin. Biotechnol J. Proteome Res Rapid Commun. Mass
1 P2 P P3P4 P5P8 P9P10 P11 P12
1 P2 P14 2 3 4 5 1 P3P4 P5P8 P9P10 P11 P12 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 & 11 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 2 1! 3 2 3! 4 4 3 5 6 I 7 8 P7 P7I P5 9 P5! 10 4!! 11 5 03-5220-8520
drug development Toshimi Michigami, E-mail : michigami@mch.pref.osaka.jp Keiichi Ozono, E-mail : keioz@ped.med.osaka-u.ac.jp The progress in the research on vitamin D 1) McCollum, E. V. et al.:
untitled
ORYZA CERAMIDE -PTPCDP8TP20CD -WSPWSP8 -LL0.8 -PCPC8PC20 -WSPCWSPC8 -LCLC0.8 Ver.13.0HS ORYZA CERAMIDE Ver.13.0HS 1. 2. 20 NMR 4 ( 1) 1 Ver.13.0HS HO CH 2 OH O O OH OH OH C CH CH H 2 NH C O R C H C H (CH
11月号
2001. http://www.wako-chem.co.jp/ NOV. No.35 C O N T E N T S Halomonas elongata Marinococcus marinus BBBBBBBBBBBBBBBBBBBBBBBBBBB in vitro 2 WAKO BIO WINDOW No.35 NOV.2001 M M M M M M M M M MM M l MMM M
DynalTransplantDiagnostic.indd
2011 1 SeCore HLA SBT 1 2 AllSet + Gold SSP HLA 7 3 SSP UniTray HLA 11 4 27 5 32 ii 1 SeCore HLA SBT SeCore 2 SeCore HLA SBT SeCore 3 SeCore 4 HLA-I 5 HLA-II 5 utype 5 GSSP 6 1 SeCore HLA SBT Sequencing
Various function and toxicity of iron Key words 1) Hentze, M. W. et al: Cell, 117, 285-297 (2004) 2) Ganz, T.: Blood, 102, 783-788 (2003) 3) Nemeth, E. et al.: Science, 306, 2090-2093 (2004) 4) Hentze,
1.WizardPlusSV日本語ﾌﾟﾛﾄｺｰﾙ
Wizard Plus SV Minipreps DNA Purification System No. TB281J 2001 7 A1270, A1330, A1340, A1460 A1470 Wizard Plus SV Minipreps DNA Purification System (a) DNA 30 20,000bp DNA Recombinant RNasin Ribonuclease
I
I II 1 2 3 4 5 6 7 8 9 10 11 Rp.1) 50mg 3 3 14 Rp.2) 2.5mg 2 14 Rp.1) 2.5mg 3 2 2:1 14 Rp.) 15g 1 3 28 Rp.2) 50 600mg 1 2 28 12 Rp.1) CR 20mg 1 1 1 14 Rp.2) R 200 1 2 14 Rp.3) 3 1 3 14 Rp.1) 2.5mg 0.4
10 117 5 1 121841 4 15 12 7 27 12 6 31856 8 21 1983-2 - 321899 12 21656 2 45 9 2 131816 4 91812 11 20 1887 461971 11 3 2 161703 11 13 98 3 16201700-3 - 2 35 6 7 8 9 12 13 12 481973 12 2 571982 161703 11
0.45m1.00m 1.00m 1.00m 0.33m 0.33m 0.33m 0.45m 1.00m 2
24 11 10 24 12 10 30 1 0.45m1.00m 1.00m 1.00m 0.33m 0.33m 0.33m 0.45m 1.00m 2 23% 29% 71% 67% 6% 4% n=1525 n=1137 6% +6% -4% -2% 21% 30% 5% 35% 6% 6% 11% 40% 37% 36 172 166 371 213 226 177 54 382 704 216
本文／ＹＡ９２５５Ｃ
pp. E- -SH X in vitro in vivo -SH Structure-based drug development and medical biological application of cathepsin specific inhibitors Nobuhiko Katunuma Institute for Health Sciences, Tokushima Bunri University,Nishihamabouji,
yakugaku-kot.ppt
2009 Masaaki Kotera kot@kuicr.kyoto-u.ac.jp 2 I II / A () B1 () B2 B12 C () D A D () () () () DNA 5- http://www.genome.jp/kegg/pathway.html KEGG PATHWAY Database Xenobiotics biodegradation http://www.genome.jp/kegg/pathway.html
64) M.L. Gefter, R.L. Russell: J. Mol. Biol., 39, 145 (1969) 65) F. Harada, F. Kimura and S. Nishimura: Biochim. Biophys. Acta., 182, 590 (1969) 65a) F.H.C. Crick: J. Mol, Biol., 19, 548 (1966); M. Nirenberg
1 2 2 4 6 6 6 7 7 7 8 8 8 13 15
2014/09/30 1 2 2 4 6 6 6 7 7 7 8 8 8 13 15 40 50 46 40 23 3 11 28 4 4 1 30 6 11 31 5 32 4 7 16 4 22 4 29 30 4 46 12 47 10 56 4 13 12 15 100 20,312 7,609 12,703 3 3,086 723 280 46 47 46 FRP 835 25m11.5m
ISO
ISO ISO ISO(International Standardization Organization) 135 ISO CD(Committee Draft) DIS(Draft for International Standard) FDIS(Final DIS) ISO ISO23500 2004.01.13 ANSI/AAMI RD52 2004.04.13 6 2004.08.09
ROWCatPathology_J.book
www.dako.jp IHC Solution Visualization Systems Number of Steps 5 4 3 2 1 LSAB TM 2 EnVision TM G 2 Doublestain EnVision TM DuoFLEX Doublestain LSAB TM + EnVision TM G 2 EnVision TM + EnVision TM FLEX 1
色覚（初組4）.pm
733 3 1 520 1 8 4 0.2500 1 30012 1 AB 3 22 3 1 2 http://www.nig.ac.jp/labs/devgen/mou.html 734 1.1 360nm 830nm 380nm 780nm 540nm 580nm 660nm 540nm 660nm Log relative corneal sensitivity 0 1 2 400 500 600
温泉の化学 １
H O 1,003 516 149 124 2,237 1974 90 110 1km 2,400 ( 100 Mg 200 (98 ) 43,665 mg 38,695 mg 19,000 mg 2000 2000 Na-Ca-Cl 806 1970 1989 10 1991 4 ph 1 981 10,000 1993... (^^; (SO_4^{2-}) " " 1973-1987 1970
46 12 3 1 ATP ( ) ATP ~P 1~P hyd Gº 1 1 1950 ~P hyd Gº Hansia et al. Biophys Chem 119: 127, 2006 ~P hyd Gº 1? ATP hyd Gº ATP ATP ATP ~P ε ~P George [BBA 223: 1, 1970] ~P 2) hyd Gº 1. ph Mg 2+ 2. 1 2 2
VOL33NO.3/2010 A B A B p-erk1/2 Time (min) 0 1 2 5 10 15 30 60 90 120 Erk1/2 Time (min) 0 1 2 5 10 15 30 60 90 120 p-vasp VASP C D enos/gapdh x-fold vs sham 2.0 1.5 1.0 0.5 * 0 ANP (-) (+) (-)
エピトープタグ抗体製品リスト PM020 Anti-DDDDK-tag pab Polyclonal Rabbit Ig (aff.) WB, IP, IC 100 ml 15,000 PM020-7 Anti-DDDDK-tag pab HRP-DirecT Polyclonal Rabbit I
(kda) 150 100 DDDDK-tagged protein 75 50 37 IgG Heavy chain 25 20 IgG Light chain Sample: N-terminal DDDDK-tagged β-gal protein transfectant μ μ μ http://ruo.mbl.co.jp/ エピトープタグ抗体製品リスト PM020 Anti-DDDDK-tag
untitled
in 2008/11/24 8020 2 3 4 11 12 35 5 Japan Dental Association 6 7 8 PMTC 9 10 11 12 13 14 Japan Dental Association 15 TNF - α 5mm 20 72 cm 2 TNF - α LPS 16 TNF- LPS TNF-LPS TNF- 17 18 19 20 21 70 22 70
Taro10-mizushima01.PDF
- 1 - ATAGO N- % % g % g % g % g % g % g Brix% g g ml ml mgm / l ml mg mol/l mol/l mol/l ph NaSO mol/ l ml / g mg mg ml mol/l ml ml L- L- FIBE-MINI FIBE-MINI % % FIBE-MINI % g % g % g % C mg mg mg
HotStarTaq Plus PCRプロトコールとトラブルシューティング( /2008)
February 2008 HotStarTaq Plus PCR HotStarTaq Plus DNA Polymerase HotStarTaq Plus Master Mix Kit PCR HotStarTaq Plus DNA Polymerase PCR 2 HotStarTaq Plus DNA Polymerase Q-Solution PCR 6 HotStarTaq Plus
SEC (SEC) SEC SEC SEC 2
SEC (SEC) SEC SEC SEC 2 SEC ( 1) 1: SEC www.agilent.com/chem/jp 3 ( ) ( 2) ICH (Q6B) 3 lgg 8 7 6 5 4 1. 2. 3. 4. 5. Agilent AdvanceBio SEC 3 Å 7.8 x 3 mm 2.7 µm (p/n PL118-531) Agilent 126 Infinity LC
Review Fumio Imamoto1, Takefumi Sone1, Kazuhide Yahata1, Yukari Sasaki1, Masatoshi Takagi2, Kazuhiro Maeshima2, Naoko 25) Dong, W. K. et al.: Gene, 91, 217-223 (1990) 26) Masatsugu, T. et al.:
ml ml ml mg ml ml mm mm mm mm mm mm mm mm mm mm mm ml mmhg mmhg ml Acetylcholine ml mg mm mm mm mm mm mm ope ++ Uribe Davis S H S S : : : : : : : : : G
Spatio-temporal regulation of ERK1/2 MAP kinase 20) Murphy, L. 0., MacKeigan, J. P., Blenis, J.: Mol. Cell. Biol., 24, 144-153 (2004) 21) Harada, T., Morooka, T., Ogawa, S., Nishida, E.: Nature Cell
.i...j...[.X8-2
Vol. 8 No.2 Nov. 2007 1 2 4 12 20 37 45 54 56 62 1 2 3 4 5 7 6 8 9 10 11 13 12 15 14 17 16 19 18 21 20 Hatena 23 22 Oidiodendron Oidiodendron 25 24 Monosiga ovata Monosiga 27 26 Toxoplasma gondii 29 28
TaKaRa Bradford Protein Assay Kit
研究用 TaKaRa Bradford Protein Assay Kit 説明書 v201701da TaKaRa Bradford Protein Assay Kit は Coomassie Dye を用いる Bradford 法に基づいたキットであり 簡単な操作で迅速に 濃度範囲が 1 ~ 1,000 μg/ml のタンパク質溶液の定量を行うことができます 本キットの定量の原理は Coomassie
2009 Aida et al. Caries Res 2006;40 2000 100 % 78.7 88.0 96.6 98.8 98.8 98.8 100.0 100.0 100 75 69.4 50 75.3 74.8 73.3 73.1 73.0 72.4 71.8 71.7 51.7 40.2 69.4 68.8 73.6 25 22.3 32.8 21.9 22.9 22.1
15K00827 研究成果報告書
(TG) TG sterol regulatory element- binding protein (SREBP)-1c (Diabetes 53:560-569, 2004) SREBP-1c (1) SREBP-1c SREBP-1c liver X receptor (LXR)LXR O-Glc NAc (O-linked -N-acetyl glucosamine) (J Biol Chem
Vitronectin EIA Kit
製品コード MK102 研究用 Vitronectin EIA Kit 説明書 ビトロネクチン (Vitronectin:VN) は 補体結合蛋白質 S- プロティンとしても知られている血中の主要な接着タンパク質で 血中には高濃度に存在しています ビトロネクチンは ビトロネクチンレセプターを保有する細胞に対して接着活性を持つ他 コラーゲン へパリンなどの物質 またプラスミノーゲン活性化阻害因子 -1
090825_Sakate_for_dist.pptx
21 子 比 Evola 用 http://www.h-invitational.jp/evola/ 手龍一 2009 8 月 25 日 火 心 H-InvDBEvola 子 子 子 子 子 日 比 Evola 用 比 子 Evola 方 比 子 Evola 子 H-InvDB 6 子 比 子 生 子 生 生 片 方 比 長 大 子 生 長 生 22,496 子 13 生 長 魚 比 http://www.h-invitational.jp/g-compass/
<4D F736F F D B BA908593B98AC782AB82E593E082CC88C091538AC7979D82C98AD682B782E992868AD495F18D908F912E646F63>
12 62 1800 14 3 11 14 4 14 4 30 ... 1... 1... 1... 2... 5... 6... 7... 10... 13... 18... 19 21 22 24 27 28 31 32 33... 34... 35... 37 2 1,650mm () H=2,500W=2,000 460m 750m 500m 42.1m 1.15m () 100% 80%
ŁRŁ¸•ñŁŁŁ‘
16 11 5 25mg 14 11 18 1 25mg 1 150,000 934 1 2 : 1-235-tumor necrosis factor receptor (human) fusion protein with 236-467-immunoglobulin G1 (human γ1-chain Fc fragment), dimmer : : 1 235 236 467 G 1 γ1
2004
2008 3 20 400 1 1,222 7 1 2 3 55.8 54.8 3 35.8 6 64.0 50.5 93.5 1 1,222 1 1,428 1 1,077 6 64.0 52.5 80.5 56.6 81.5 30.2 1 2 3 7 70.5 1 65.6 2 61.3 3 51.1 1 54.0 2 49.8 3 32.0 68.8 37.0 34.3 2008 3 2 93.5
- 1 - - 2 - - 3 - - 4 - - 5 - - 6 - - 7 - - 8 - - 9 - - 10 - - 11 - - 12 - - 13 - - 14 - - 15 - - 16 - - 17-54 55 56 57 58 ( ) 54 7 55 56 57 58 10 54 55 57 58 56 25 27 25 27 20 22 25 22 1.4g 23 23 :1.8g
07.報文_及川ら-二校目.indd
8 01 01 4 4 1 5 16 18 6 006 H 18 4 011 H 6 4 1 5 1 5 007 H 19 5 009 1 5 006 007 009 011 9 10 4 000 H 1 4 5 004 H 16 4 004 009 H 1 5 4 4 5 1 4 006 011 1 1 4m 5m 10m 007 1 7 009 009 1 5 10 1 000kg 10a 006
AllPrep DNA/RNA Micro プロトコールとトラブルシューティング ( /2008)
July 2007 AllPrep DNA/RNA Micro DNA RNA 5 x 10 5 5 mg Sample & Assay Technologies DNA RNA 3 DNA RNA 11 DNA RNA 19 24 2 AllPrep DNA/RNA Micro 07/2007 DNA RNA RNA AllPrep DNA Spin Column DNA RNeasy MinElute
秋田応用微生物研究会
13:00-13:10 13:10-13:30 13:30-13:50 (Tricholoma auratum) 13:50-14:10 14:10-14:30 14:30-14:50 14:50-15:10 α-msh 15:10-15:30 Lewis 15:30-15:50 15:50-16:10 16:10-16:30 (1) (2) ( ) (2) (1) (2) (3) (1) (2)
食糧 その科学と技術 No.43( )
53 inclusion body: 75 20 1 2 3 90 2000 CA 2001 CA 54 1 Refolding on Resin Dilution additive methods 2 1 2 3 4 1990 55 3 CA 4 5 6 17-1,4- CA CA 7 CA 20 DnaJ, DnaK, GroEL/ES 1 CA 2 CA 3 2 1 6M 2 56 4 1 2
H1-P15
72 January 2009 72 January 2009 72 January 2009 2008 GFP GFP 1992 Prasher cdna GFP Aequorea victoria GFP cdna in vivo EGFP GFP FACS GFP GFP GFP GFP 1. Prasher, D.C., et al. 1992. Primary structure of the
まえがき
JAAS0012006 1 -------------------------------------------------------------------------------------------------------- 1 2 --------------------------------------------------------------------------------------------------------
JP.qxd
Agilent 2100 1 DNA RNA Lab-on-a-Chip Agilent 2100 1999 Agilent 2100 Lab-on-a-Chip Agilent 2100 RNA DNA SDS-PAGE Agilent 2100 Agilent 2100 Agilent 2100 : (1 4 µl) 30 21 CFR Part 11 Agilent 2100 Agilent
ライフサイエンス関連 コラーゲン・ゼラチン製品案内 2015.12
3 mg/ml ph 3 4 8 1 Type I-A Type I-P Type I-A Type I-C Type III Type III Type IV Type IV TEL 072-820-3079 FAX 072-820-3095 4 8 4 Type I-A 20mL 1 F-12 MEM 5mL 1 4mL 5 10F-12MEM DFDME F-12 1 1199 5 DMERPMI-1640
14 2 5 20 21 DNA 1 1 2 () () 3 4 5 4 J.R.Webster, M.A.Burms, D.T.Burke and C.H.Mastrangelo Center for Integrated Microsystems, Department of Electrical Engineering and Computer Science, Department of
肺癌第50巻第4号
1 Department of Cancer and Thoracic Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan; 2 Department of Thoracic Surgery, Aichi Cancer Center, Japan;
プロトコール集 ( 研究用試薬 ) < 目次 > 免疫組織染色手順 ( 前処理なし ) p2 免疫組織染色手順 ( マイクロウェーブ前処理 ) p3 免疫組織染色手順 ( オートクレーブ前処理 ) p4 免疫組織染色手順 ( トリプシン前処理 ) p5 免疫組織染色手順 ( ギ酸処理 ) p6 免疫
< 目次 > 免疫組織染色手順 ( 前処理なし ) p2 免疫組織染色手順 ( マイクロウェーブ前処理 ) p3 免疫組織染色手順 ( オートクレーブ前処理 ) p4 免疫組織染色手順 ( トリプシン前処理 ) p5 免疫組織染色手順 ( ギ酸処理 ) p6 免疫組織染色手順 ( ギ酸処理後 マイクロウェーブまたはオートクレーブ処理 )p7 抗原ペプチドによる抗体吸収試験 p8 ウエスタン ブロッティング
untitled
COLAK Mehmed 11000 305 305 180 10000 9000 8000 160 140 120 7000 100 6000 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 80 GH IGF-1 GH GH 5 4 3 2 1 0 2 3 4 5
2902 Shiraki et al., Aerosol and Air Quality Research, 17: , 2017 McLean, 1961; Schulman and Kilbourne, 1962; Moser et al., 1979; Knight, 198
PM 2.5 PM 2.5 UV-LED UV-LED 375 nm TiO 2 TiO 2 5 10 1 cm 200 5 ppm 9012.4 µmol h -1 1:2 2 25.43 µmol h -1 1 m 3 20 ppm 8 60 779 L A/PR/8/1934 H1N1 RNA 7 28 RNA PM2.5 TiO2 PM 2.5 VOC Hess et al., 2016;
untitled
RYZA IL & FAT CHEMICAL C., LTD. SAKURA EXTRACT Ver.1.4MK Ver. 1.4MK 1) 1300 1929 1 Ver. 1.4MK Sakura 1-caffeoyl---D-glucopyranoside quercetin 3---D-glucopyranoside 1) :, 4, 11-22 (1995). 2 Ver. 1.4MK 3
ッ ー ー ー() () 2.5 () () 21 1) -1 2) -2 10 m 5 7 23 6 1)2) 3) -1-2 H23.4.25 -15-20 4 75 5 1) -1 (5) (3) (3) (2) (1) (1) (1) (1) (1) (1) (1) (1) (1) (1) (1) (1) 1) ( 23 6 8 ) 2) 22 3)
12-7 12-7 12-7 12-7 12-8 12-10 12-10 12-10 12-11 12-12 12-12 12-14 12-15 12-17 12-18 10 12-19 12-20 12-20 12-21 12-22 12-22 12-23 12-25 12-26 12-26 12-29 12-30 12-30 12-31 12-33 12-34 12-3 12-35 12-36
Kazumitsu Ueda, [Department of Applied Life Science, Kyoto University Graduate School of Agriculture, Kitashirakawa, Sakyo-ku, Kyoto 606-01, Japan] Molecular Mechanisms for Drug Transport by MDR1/P-glycoprotein
untitled
- 1 - - 2 - OFF - 3 - 18-4 - - 5 - - 6 - (1) (2) No 1 0099240130 CL-10 2 0099240040 CL-3-7 - - 8 - 50Hz 250 / - 9 - 6. 1 6. 2 R 6. 3 V 6. 4 R - 10 - 6. 5 6. 6 6. 7-11 - 6. 8 500mm 6. 9 4. 6. 10-12 - 6.
untitled
er. 3.0SJ ver.3.0 SJ 3 2 1 ver.3.0 SJ 900 1200 () 400 2 ver.3.0 SJ OH OMe OMe O O O MeO O MeO OMe O O OMe O OMe OMe O O MeO O O H O O MeO OMe O H O OH OMe O CH 2 HO O O O O CH 3 OH HO OH OH HO OH O 1 3
BE0005 TS-1/ InVivoMab anti-human LFA-1α (CD11a) mouse IgG , ,000 BE0005 TS-1/ InVivoMab anti-human LFA-1α (CD11a) mous
Bio X Cell 社価格表 (2018 年 4 月 ) < 株式会社シン コーポレーション > Bio X Cell 社価格表 (2018 年 4 月 ) ご注文ごとに別途取扱手数料 (2( 万円 税別 ) を申し受けます 大学向け価格でのご購入には アカデミックプライス適用申込書のご提出が必須となります 品番クローン品名イソタイプ 容量 (mg) 大学向希望小売価格 ( 円 ) 税別 希望小売価格
PowerPoint プレゼンテーション
ELISA 用プレート ( フ ロッキンク レスタイフ ) ( 旧称 :New ELISA plate) 住友ベークライト株式会社 S- バイオ事業部 - 1 - ELISA 用プレート ( フ ロッキンク レスタイフ ) の特長 当社独自の表面処理技術によりブロッキング 操作なしで低いバックグランドを提供します 血清などに含まれる種々タンパク質の非特異吸着を抑制します 予めブロッキング処理がされている為
橡ミヤコグサ形質転換法・秋山
Page 1 2000 9 akiyama@biochem.osakafu-u.ac.jp VA 4 1999 9 2000 2 Plant J., 2, 487-496 (1992) Handberg K. and Stougaard J. Exp. Bot., 48,1357-1365 (1997) Stiller J. (Gresshoff PM) 4 80 5 10 20 ( ) < ecotype
4) H. Takayama et al.: J. Med. Chem., 45, 1949 (2002). 5) H. Takayama et al.: J. Am. Chem. Soc., 112, 8635 (2000). 6) H. Takayama et al.: Tetrahedron Lett., 42, 2995 (2001). 9) M. Kitajima et al: Chem.
9 3 12 17 15 19 10 19 13 16 16 17 11 17 14 16 17 19
9 3 12 17 15 19 10 19 13 16 16 17 11 17 14 16 17 19 4 4 5 11 11 13 1,739m 1,722m 25km 17km 6,817 1,052m 1,018m 14 1,858,070 668,446 35 1,101,872 59 4 5 6 22 1889 7 20 52 228 1619 5.3.17 32.5 130.6