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文部科学省 科学研究費補助金 新学術領域研究 修飾シグナル病 第二回公開シンポジウム 修飾シグナル病 学術領域の新展開 抄録集 プログラム 2012年1月28日 土 13:00 17:30 東京大学医科学研究所 1号館1F講堂 領域代表者 井上純一郎

2 2012 1 28 13:00-13:10 13:10-15:10 1...2...4...6...8 15:30-17:20 2...10...12...14 17:40-...16 1

13:10-13:40 NF-κB NF-κB IκB kinase (IKK) NF-κB IκB IκB NF-κB IKK IKK IκB IKK NF-κB IKK IKK T I (HTLV-I) Tax IKK NEMO IKK Tax IKK NIK NIK NF-κB TRAF6 RIP1K A20 IKK NF-κB HTLV-I IKK A20 A20 B A20 NF-κB 1. Saitoh, Y. et al., Lung Cancer, 70, 263-70, 2010. 2. Saitoh, Y. et al., Blood, 111, 5118-5129, 2008. 3. Nonaka, M. et al., Oncogene, 24, 3976-3986, 2005 4. Saitoh, T. et al., J. Immunol., 174, 1507-12, 2005. 1982 1983 1994 1996 1999 2007 2

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13:40-14:10 1) SILAC Stable Isotope Labeling by Amino acids in Cell culture EGF Src 2) MCF-7 3) 1. Oyama M, Kozuka-Hata H, Suzuki Y, Semba K, Yamamoto T and Sugano S. Diversity of translation start sites may define increased complexity of the human short ORFeome. Mol Cell Proteomics, 6: 1000-1006 (2007). 2. Oyama M, Kozuka-Hata H, Tasaki S, Semba K, Hattori S, Sugano S, Inoue J and Yamamoto T. Temporal perturbation of tyrosine-phosphoproteome dynamics reveals the system-wide regulatory networks. Mol Cell Proteomics, 8: 226-231 (2009). 3. Oyama M, Nagashima T, Suzuki T, Kozuka-Hata H, Yumoto N, Shiraishi Y, Ikeda K, Kuroki Y, Gotoh N, Ishida T, Inoue S, Kitano H and Okada-Hatakeyama M. Integrated quantitative analysis of the phosphoproteome and transcriptome in tamoxifen-resistant breast cancer. J Biol Chem, 286: 818-829 (2011). 2004 2007 2010 2011 4

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14:10-14:40 1 (IGF-1) IGF-1 IGF-1 PI3K Akt GSK-3β nebulin (Neb) C Z GSK-3β Neb SH3 N-WASP Pro-rich N-WASP Z Neb N-WASP WH2 Z Neb 1 µm 1 µm Neb C NEB Neb N-WASP Neb 0.15 µm nebulette (Nebt) 1 µm N-WASP Nebt C Z Nebt N-WASP Nebt 0.15 µm Nebt N 0.85 µm 1 µm NEBT Nebt 1. Takano, K. et al. Science 330: 1536 1540 (2010). 2. Watanabe-Takano, H. et al. Exp. Cell Res. 316: 477 490 (2010). 3. Yokoyama, T. et al. J. Cell Biol. 177: 781 793 (2007). 4. Endo, T. Regen. Med. 2: 243 265 (2007). 5. Sun, P. et al. Genes Cells 11: 1097 1113 (2006). 1984 2005 6

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14:40-15:10 E-box DNA 24 CACGTG E-box bhlh-pas CLOCK BMAL1 E-box Per Cry PER CRY CLOCK-BMAL1 CLCOK bhlh E-box bipartite (3) CRY (2) SREBP E4bp4 (1) TGF-β ALK5 SMAD2/3 Dec1 (4) 1. Hatori et al. Proc. Natl. Acad. Sci. USA. 108, 4864-4860 (2011). 2. Kurabayashi, N. et al. Mol. Cell Biol. 30, 1757-1768 (2010) 3. Yoshitane, H. et al. Mol. Cell Biol. 29, 3675-3686 (2009) 4. Kon N. et al. Nat. Cell Biol. 10, 1463-1469 (2008) 1983 1995 8

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15:30-16:10 GTP FRET FRET FRET 1. K. Aoki, M. Yamada, K. Kunida, S. Yasuda, and M. Matsuda. Processive phosphorylation of ERK MAP kinase in mammalian cells. Proc Natl Acad Sci U S A 108:12675-12680, 2011. 2. N. Komatsu, K. Aoki, M. Yamada, H. Yukinaga, Y. Fujita, Y. Kamioka, and M. Matsuda. Development of an optimized backbone of FRET biosensors for kinases and GTPases. Mol Biol Cell 22 (23):4647-4656, 2011. 3. E. Hirata, H. Yukinaga, Y. Kamioka, Y. Arakawa, S. Miyamoto, T. Okada, E. Sahai, and M. Matsuda. In vivo fluorescence resonance energy transfer imaging reveals differential activation of Rho-family GTPases in glioblastoma cell invasion. J Cell Sci, in Press. 1987 2006 2007 10

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16:10-16:50 2 2 DNA DNA 2 rule of thumb 1 1 1 3 2-300nm 3 1 40-300nm 2 2-20nm 3 5-50nm 1 A. Kusumi et al. Traffic 5, 213-230 (2004). A. Kusumi et al. Annu. Rev. Biophys. Biomol. Struct. 34, 351-378 (2005). A. Kusumi et al. Trends Biochem. Sci. 36, 604-615 (2011). 12 1980 2007 - icems

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16:50-17:30 2 32 P ph Phos-tag Phos-tag 1 Phos-tag Kinoshita, E. et al. Mol. Cell. Proteomics 5, 749 757 (2006) Kinoshita, E. and Kinoshita-Kikuta, E. Proteomics 11, 319 323 (2011) Kinoshita, E. et al. Proteomics (in press) [doi: 10.1002/pmic.201100524] 1997 2003 14

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17:40-16 NLK Lef1 Wnt/ NLK positively regulates Wnt/betacatenin signaling byphosphorylating Lef1 in neural progenitor cells The Nestin cell lineage is responsible for the lethal phenotypes of Girdin straight knockout mice. DYRK2 c-jun/c-myc G1/S In silico Spatio-temporal Simulation of NF-κB Oscillation Oscillationtion of non-canonical NF-κB pathway Kinetic analysis of persistent NF-κB activation during PMA-induced differentiation of human monocytic THP-1 cells Ubiquitin-editing enzyme A20 contributes to the survival of cancer cells Akt/PKB Plk1 ph Phos-tag SDS- PAGE A20 zinc finger7 LUBAC NF-κB PGAM5 A20 zinc finger7 NASH) Pin1 PAR14 HOILI-1L VCP DJ-1 p53 MAPK Rab6A Stress-inducible caspase substrate TRB3 promotes nuclear translocation of procaspase-3 CCP1 EGFR Ser/Thr MAPKKK A role of Pin1 in neural differentiation TRIM Terf Dok-7 Critical roles of a metalloendopeptidase nardilysin in cold-induced adaptive thermogenesis CRY2 Activation of PKR by ubiquitin-like molecule ISG15 modification (ISGylation). FGF