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1 Atg Atg Mizushima, N.Cell Death Differ.,,. Nakatogawa, H., Ichimura, Y., & Ohsumi, Y.Cell,,. Baba, M., Takeshige, K., Baba, N., & Ohsumi, Y.J. Cell Biol.,,. Baba, M., Osumi, M., & Ohsumi, Y. Cell Struct. Funct.,,. Mizushima, N., Yamamoto, A., Hatano, M., Kobayashi, Y., Kabeya, Y., Suzuki, K., Tokuhisa, T., Ohsumi, Y., & Yoshimori, T.J. Cell Biol.,,. Ishihara, N., Hamasaki, M., Yokota, S., Suzuki, K., Kamada, Y., Kihara, A., Yoshimori, T., Noda, T., & Ohsumi, Y. Mol. Biol. Cell,,. Hamasaki, M., Noda, T., & Ohsumi, Y.Cell Struct. Funct.,,. Suzuki,K.&Ohsumi,Y.FEBS Lett.,,. Suzuki, K., Kubota, Y., Sekito, T., & Ohsumi, Y. Genes Cells,,. Mizushima, N., Noda, T., Yoshimori, T., Tanaka, Y., Ishii, T., George, M.D., Klionsky, D.J., Ohsumi, M., & Ohsumi, Y. Nature,,. Ichimura, Y., Kirisako, T., Takao, T., Satomi, Y., Shimonishi, Y., Ishihara, N., Mizushima, N., Tanida, I., Kominami, E., Ohsumi, M., Noda, T., & Ohsumi, Y.Nature,,. Kirisako, T., Ichimura, Y., Okada, H., Kabeya, Y., Mizushima, N., Yoshimori, T., Ohsumi, M., Takao, T., Noda, T., & Ohsumi, Y.J. Cell Biol.,,. Kabeya, Y., Mizushima, N., Ueno, T., Yamamoto, A., Kirisako, T., Noda, T., Kominami, E., Ohsumi, Y., & Yoshimori, T. EMBO J.,,. Ichimura, Y., Imamura, Y., Emoto, K., Umeda, M., Noda, T., & Ohsumi, Y.J. Biol. Chem.,,. Chernomordik, L.V. & Kozlov, M.M.Cell,,. Mechanisms of membrane biogenesis in autophagy Hitoshi NakatogawaDivision of Molecular Cell Biology, National Institute for Basic Biology, Nishigonaka Myodaijicho, Okazaki, Aichi, Japan CIA DNA nucleosome core particle HAHBHH DNA DNA DNA DNA DNA DNA DNA DNA ATP ATP ATPase SWISNFISWIRSC CHDSWR ATP DNA DNA ATP NAPFACT HA HB TAF-ICAF- HIRACIAFKBPNN HH

2 ATP CIA HH CIA CIA CIA CIA TFIID CCGcell cycle geneccginteracting factor A CIA H H H C CIA Sternglanz anti-silencing function ASF Kadonaga CAF- RCAF CIA HH CIA CIA TFIID DNA HIRA DNA CAF- DNA RFC CIA ORF DNA CIA CIA HH CIA CIA CIA NMR CIA NF-κB p DNA CIA HH HH CIA CIA HH CIA HH Tyler CIA HH CIA HH a d CIA HH CIA HH CIA HH CIA HH e HH HH CIA CIA HH HH abhh H α α acia HH CIA bcia H HHH

3 1 0 7 0 生化学 第7 9巻 第1 1号 みにれびゆう

4 CIA HH CIA HH a bcia HHCIA HH primary binding sitepbs secondary binding sitesbsb a c d CIA c PBS d SBS cd ab e!ls RILSRIHH peak CIA peakhhcia HHpeak CIA HH a HbCIA HH CIA H PBSc HA Hd CIA HH CIA HSBS ahh HH DNA HH CIA HH HH HH HH DNA HAHB HAHB b c HH H H CIA HH CIA HH H H bc ab CIA HH H H C βcβch HA C β β c CIA HH βch CIA β β d HH HAHB HAHB FACT HH βch CIA HH CIA HHHH CIA Yawara split DNA HH HH DNA a HH CIA HH

5 HH HH DNA a CIA HH bc HH DNA DNA DNA HH CIA HH CIA HH CIA HH CIA HH DNA CIA HH CIA English, C.M., Adkins, M.W., Carson, J.J., Churchill, M.E., & Tyler, J.K.Cell,,. Natsume, R., Eitoku, M., Akai, Y., Sano, N., Horikoshi, M., & Senda, T.Nature,,. Munakata, T., Adachi, N., Yokoyama, N., Kuzuhara, T., & Horikoshi, M.Genes Cells,,. Chimura, T., Kuzuhara, T., & Horikoshi, M.Proc. Natl. Acad. Sci. USA,,. Le, S., Davis, C., Konopka, J.B., & Sternglanz, R. Yeast,,. Tyler, J.K., Adams, C.R., Chen, S.R., Kobayashi, R., Kamakaka, R.T., & Kadonaga, J.T.Nature,,. Daganzo, S.M., Erzberger, J.P., Lam, W.M., Skordalakes, E., Zhang, R., Franco, A.A., Brill, S.J., Adams, P.D., Berger, J.M., & Kaufman, P.D.Curr. Biol.,,. Tyler, J.K., Collins, K.A., Prasad-Sinha, J., Amiott, E., Bulger, M., Harte, P.J., Kobayashi, R., & Kadonaga, J.T.Mol. Cell Biol.,,. Franco, A.A., Lam, W.M., Burgers, P.M., & Kaufman, P.D. Genes Dev.,,. Adkins, M.W., Howar, S.R., & Tyler, J.K.Mol. Cell,,. Schwabish, M.A. & Struhl, K.Mol. Cell,,. Mello, J.A., Silljé, H.H., Roche, D.M., Kirschner, D.B., Nigg, E.A., & Almouzni, G.EMBO Rep.,,. Padmanabhan, B., Kataoka, K., Umehara, T., Adachi, N., Yokoyama, S., & Horikoshi, M.J. Biochem. (Tokyo),,. Mousson, F., Lautrette, A., Thuret, J.Y., Agez, M., Courbeyrette, R., Amigues, B., Becker, E., Neumann, J.M., Guerois, R., Mann, C., & Ochsenbein, F.Proc. Natl. Acad. Sci. USA,,. Tagami, H., Ray-Gallet, D., Almouzni, G., & Nakatani, Y. Cell,,. Mechanism of nucleosome assemblydisassembly mediated by histone chaperone CIA; Implications for nucleosome semi-conservative replication Ryo Natsume, Masamitsu Eitoku, Masami Horikoshi and Toshiya Senda Japan Biological Information Research CenterJBIRC, Japan Biological Informatics Consortium JBIC, AIST Bio-IT Research Building,Aomi, Koto- Ku, Tokyo, Japan, Institute of Molecular and Cellular BiosciencesIMCB, The University of Tokyo, Biological Information Research CenterBIRC, National Institute of Advanced Industrial Science and Technology AIST, AIST Bio-IT Research Building,Aomi, Kotoku, Tokyo, Japan

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