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2 Actinomycetologica The International Conference of Natural Product Biosynthesis The Journal of Antibiotics Web Digital Atlas of Actinomycetes 26
3 Actinomycetologica Actinomycetologica Actinomycetologica 20 B5 A4 J-STAGE The Journal of Antibiotics Actinomycetologica 1
4 Bergey's Manual 2 nd ed. vol. 5 Actinobacteria Phylum Actinobacteria Class 15 Order 43 Family 203 Genus Harz Actinomyces Bergey s Manual Microbacterium HV agar 1987 HV agar ISI Web of Knowledge SM Digital Atlas of Actinomycetes Digital Atlas 2
5 2011 Construction of a natural product library containing secondary metabolites produced by actinomycetes Motoki Takagi and Kazuo Shin-ya The Journal of Antibiotics (2012) 65, Regulatory mechanisms of light-inducible transcription in non-phototrophic bacteria Hideaki Takano Actinomycetologica (2012) 17, S3-S8 3
6 O-1 Frankia O-2 Rhodococcus erythropolis 1, 2 1, 2 ( 1 2 ) O-3 Demequinaceae 1, NITE NBRC 2 O O-5 Nocardiopsis pyrone 10:
7 O-6 Streptomyces O-7 Streptomyces coelicolor A3(2) AfsR O-8 ATP O-9 Streptomyces griseus O-10 3 O-11 Identification of small molecules inducing reveromycin production Suresh Panthee, Shunji Takahashi, Teruo Hayashi, Takeshi Shimizu, Makoto Muroi, Toshihiko Nogawa, Yushi Futamura and Hiroyuki Osada (Chem. Biol. Dept., RIKEN ASI) O-12 D- O- -L-,,,,, O-13 D- L- -O-,,,, ( ) O-14 O-15 Labdan swapping O-16 Streptomyces sp. RI-18 benzastatin JBIC 3 AIST O-17 Streptothricin ST ST
8 JBIC 4 O-18 Streptomyces griseus StrVW P-01 Friedmanniella Microlunatus P-02 Isolation of actinomycetes from deep-sea core samples Ulanova Dana (Kochi University, Science Research Center) P ,2 1 2 P-04 P-05 Lactacystin Streptomyces sp. OM-6519 cyslabdan Streptomyces sp. K , P-06 Actinoplanes NBRC P , NBRC P-08 Streptomyces turgidiscabies 1 1 J. P. Y. Valkonen P-09 rakicidin A P-10 Streptomyces atroolivaceus berninamycin E 1 1, P-11 Streptomyces sp. MK , P-12 FR Lentzea sp. No
9 1 2 P-13 Streptomyces 1, M. E. Trujillo 2, 3, Salamanca 3 P-14 JBIR JBIC P-15 Streptomyces P-16 Quorum sensing P-17 Gordonia NITE NBRC P-18 Microbispora 1 1 Chantra Indanada 2 Arinthip Thamchaipenet 2 1 ( 1 2 Kasetsart ) P-19 Chloroflexi Thermosporothrix hazakensis P , P JBIC 2 3 P-22 Amycolamicin P-23,,,,,, P-24 III in vitro P CoA 1, , P-26 Micromonospora sp. TPMA0041 mycinosyl-izenamicin P-27 Streptomyces rochei 7
10 ( 1 ) P-28 Benzoisochromanequinone , P-29 cytochrome P450 RosC RosD in vitro Rosamicin P-30 Streptomyces coelicolor actva-orf4 actinorhodin , P-31 Streptomyces rochei SRB P-32 P-33 D- DcsA,,,, P-34 M NRPS orf7 Mario RovaniHENDRIYANTO Fitria NINGSIH ( ) P-35 Streptomyces lactacystinaeus OM-6519 lactazole A P-36 Streptomyces sp. TP-A0584 P Yan Yan, 3 3, Wen Liu, 1 ( 1 ) P P-39 Functional analysis of afsr homologue regulatory gene in Streptomyces acidiscabies producing thaxtomin A Son, D.H., Kim, M.J., Park, J.Y., Choi, S.U., and Hwang, Y.I. (Dept. Food Sci. Biotechnol., Kyungnam Univ., Republic of Korea) P-40 Streptomyces coelicolor A3(2) rpslk88e
11 P P-42 rpob P-43 Biodegradation of chitin in soil by Streptomyces coelicolor A3(2) Nazari, B. 1, Kobayashi, M. and Fujii, T. 1 ( 1 Div. Environ. Biofun., NIAES, Life Environ. Sci., Univ. Tsukuba) P-44 Rhodococcus jostii RHA1 β P-45 Sinomonas atrocyanea 1 1 P-46 P4-Ro ( 1 NBRC) P-47 Arthrobacter P-48 (A.L.) P-49 Streptomyces ( 1 3 ) P ( 1 2 ) P-51 High expression of transglutaminase gene derived from Streptomyces platensis YK-2 in Streptomyces strains Cheon, M.Y., Lee S.E., Kim, N.S., Choi, S.U., and Hwang, Y.I., (Dept. Food Sci. Biotechnol., Kyungnam Univ., Republic of Korea) P ( 1 JBIC 3 4 ) P-53 DNA 1 1 P-54 1, P-55 Rhodococcus 9
12 P-56 Analysis of the circularization of the linear chromosome of Streptomyces rochei Yosi Nindita, 1 Zhisheng Cao,v Yuh Shiwa, Hirofumi Yoshikawa, Kenji Arakawa 1 and Haruyasu Kinashi 1 ( 1 Dept. Mol. Biotechnol., Grad. Sch. AdSM, Hiroshima Univ., Dept. Biosci., Tokyo Univ. Agric.) P-57 Kitasatospora setae KM-6054 T 1,, 1,, 1,2 ( 1, ) 10
13 :30 17: Botryococcus braunii Screening of actinomycete strains based on biosynthetic genes of secondary metabolites. Hisayuki KOMAKI komaki-hisayuki@nite.go.jp Streptomyces PKS NBRC Streptomyces PKS KS PCR "S. bicolor" NBRC T PKS JBIR-13 1) "S. viridochromogenes subsp. sulfomycini" NBRC T KS JBIR-11 2) PKS PKS NRPS 3,4) PCR 11
14 1 100 pyrosequencing NBRC 600 PKS KS NRPS A PCR pyrosequencing NBRC DoBISCUIT Database of Biosynthesis clusters Curated and Integrated, /dobiscuit. html 5) KS A DoBISCUIT 1) Komaki, H. et al.: Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC as predicted by sequence analysis of type-i polyketide synthase gene. Appl. Microbiol. Biotechnol., 83, (2009) 2) Izumikawa, M. et al.: Stawamycin analog, JBIR-11 from Streptomyces viridochromogenes subsp. sulfomycini NBRC J. Antibiot., 61, (2008) 3) Khan, S.T. et al.: Streptomyces associated with a marine sponge Haliclona sp.; biosynthetic genes for secondary metabolites and products. Environ. Microbiol., 13, (2011) 4) Enkh-Amgalan, J. et al.: Diversity of nonribosomal peptide synthetase and polyketide synthase genes in the genus Actinoplanes found in Mongolia. J. Antibiot., 65, (2012) 5) Ichikawa, N. et al.: DoBISCUIT: a database of secondary metabolites biosynthesis cluster. Nucl. Acids Res. (in press) 6) :. 70, (2012) Identification and application of the bifidobacterial enzymes involving in the metabolism of human milk oligosaccharides. Motomitsu KITAOKA mkitaoka@affrc.go.jp 1 (Bifidobacterium ) (HMOs) HMOs
15 Fuc, Neu5Ac Gal 3 -N- I(LNB, Galβ1,3GlcNAc) N- (LacNAc, Galβ1,4GlcNAc) I II HMOs I I -N- (GNB, Galβ1,3GalNAc) LNB GNB/LNB GNB/LNB O GNB LNB GNB/LNB GNB/LNB ABC GNB/LNB (GLNBP) N- 1- (NahK) UDP-Glc-Hex1P (GalT) UDP-Glc4- (GalE) Bifidobacterium bifidum HMOs LNB α1,3/4- α1,2- α- -N- GNB/LNB LNB GlcNAc GLNBP LNB UDP-Glc LNB 85% LNB kg LNB LNB A B GNB/LNB 1) Kitaoka M, Adv. Nutr., 3 (3) 422S-429S (2012). 2),, 61 (2), (2012). LNB GNB 3), 50 (1), 2-5 (2012). 4) Fushinobu S, Biosci. Biotechnol. Biochem., 74 (12), (2010). 5),, 46 (8), (2008). Botryococcus braunii Biosyntheses and metabolisms of triterpene hydrocarbons produced by a green microalga Botryococcus braunii that is promising as a source of biofuel. glta gltb gltc lnpa lnpb lnpc lnpd GltA ABC GNB/LNB (GL- BP) GltB ABC GltC ABC LnpA GNB/LNB (GLNBP) (EC ) LnpB N- 1- (NahK, EC ) LnpC UDP- Glc- Hex1P- (GalT, EC ) GlcNAc/GalNAc1P, UDP- GlcNAc/GalNAc LnpD UDP- Glc/GlcNAc 4- (GalE, EC ) GNB/LNB ) ATP ADP LnpA GlcNAc + Gal1P + GalNAc LnpC Glc1P UDP-Glc LnpD UDP-Gal LnpB ATP ADP UDP-GlcNAc LnpD UDP-GalNAc GlcNAc1P GalNAc1P LnpC GlcNAc1P 1 Joe Chappell 2 Shigeru OKADA 1, Joe CHAPPELL 2 13
16 1 2 Plant Biology Program, University of Kentucky aokada@mail.ecc.u-tokyo.ac.jp Botryococcus braunii A B L A alkadiene alkatriene B botryococcene methylsqualene L lycopadiene B B botryococcene methylsqualene methylsqualene botryococcene C 30 botryococcene farnesyl pyrophosphate=fpp squalene B squalene Botryococcus Squalene Synthase=BSS cdna botryococcene BSS squalene -1 Squalene Synthase-Like protein-1=ssl-1 cdna SSL-1 FPP C 30 botryococcene squalene SSL-1 B. braunii C 30 botryococcene SSL-1 C 30 botryococcene DNA B EST BSS SSL-1 2 squalene SSL-2 SSL-3 SSL-1 FPP PSPP SSL-1 SSL-2 SSL-1 SSL-3 squalene C 30 botryococcene SSL-1 FPP PSPP SSL-2 SSL-3 PSPP squalene C 30 botryococcene B C-24 14
17 1) S. Okada, T. P. Devarenne, J. Chappell (2000) Arch. Biochem. Biophys. 373: ) S. Okada, T. P. Devarenne, M. Murakami, H. Abe, J. Chappell (2004) Arch. Biochem. Biophys. 422: ) T. D. Niehaus, S. Okada, T. P. Devarenne, D. S. Watt, V. Sviripa, J. Chappell (2011) PNAS 108: ) T. D. Niehaus, S. Kinison, S. Okada, Y-S. Yeo, S. A. Bell, P. Cui, T. P. Devarenne, J. Chappell (2012) J. Biol. Chem. 287: The Microstructure of Soil Harboring Diverse Microbes. Tsutomu HATTORI (i) aggregate mm,micro-aggregate μ (ii) NB DNB 15
18 CFC CFC CFC 1988 The physical environment in soil microbiology: An attempt to extend principle of microbiology to soil microorganisms. T. Hattori and R. Hattori, Crit. Rev. Microbiol.4: The Viable Count Quantitative and Environmental Aspects. T.Hattori, Science Tech, Madison/Springer Verlag, Berlin Distinct growth strategies of soil bacteria as revealed by large-scale colony tracking. M. Ernebjerig and R. Kishony. Appl. Environ. Microbiol. 78(5),
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26 The Journal of Antibiotics Web Actinomycetologica Official Journal The Journal of Antibiotics JA Web JA 1 HP JA xxxxxxx 3 JA 1 HP JA 2 3 JA 4 Current Issue Archive Full Text PDF 1 JA Nature Publishing Group 2 The Journal of Antibiotics Tel : 03(6809) ja@natureasia.com 24
27 25 Actinomycetologica Vol.21 No.2 (2007) Vol.24 No.2 (2010) Streptomyces coelicolor A3(2) 3,200 3,
28 Digital Atlas of Actinomycetes 2002 HP Digital Atlas of Actinomycetes www0.nih.go.jp/saj/digitalatlas/ Ref., 90, 32, 2012 Digital Atlas of Actinomycetes , Digital Atlas of Actinomycetes Digital Atlas of Actinomycetes tamura-tomohiko@ nite.go.jp NITE/NBRC Streptomyces non-streptomyces Digital Atlas of Actinomycetes 26
29 RD Meiji Seika 26 2 ACTINOMYCETOLOGICA TEL&FAX saj@lisci.kitasato-u.ac.jp TEL FAX ,000
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