食糧　その科学と技術　No.45( )

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えつみさどひら
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1 A RIN rin rin

2 PG PG PG PG

3 high pigment hp 1 hp 2 β non ripening nor ripening inhibitor rin Never ripe Nr Never ripe nor rin rin rin rin

4 LeMADS RIN Vrebarov MADS rin LeMADS RIN mrna rin rin/rin RIN/rin B RIN/rin RIN/RIN rin/rin F RIN/rin rin RIN/rin F RIN/rin F RIN rin rin RIN/rin F F

5 rin F a Kc PK TK Kc PK PK Kc PK PK Kc PK PK Kc PK PK Kc F PK Kc PK PK Kc PK PK CIE rin a F LeMADS RIN Kc Kc LeMADS RIN F F Kc KGM A KGM RIN/rin F KGM LeMADS RIN rin KGM RIN/rin

6 A F B F F Mature Green; G Breaker; B Pink; P Red ripe; R rin P R Psy Zds Pds Zds Pds rin Psy rin B F F Psy

7 β F A PG β TBG4 LeEXP1 F B F S

8 aminocyclopropane carboxylic acid ACC ACC B ACC ACS ACC ACO LeACS2 LeACS4 LeACO1 rin F A F ACC ACC

9 LeACS2 LeACS4 LeACO1 mrna F LeACS2 LeACS4 LeACO1 mrna ACS ACO mrna C F mrna C F ACS ACO LeMADS RIN RIN/rin ACS ACS F rin RIN/rin rin F F RIN/rin F RIN RIN RIN RIN

10 rin Oral allergy syndrome: OAS RIN rin RIN/rin F rin Cornell Boyce Thompson cdna rin F minor allergen beta fructofuranosidase precursor β

11 rin F F PG PG B F β PG β β PG PCR β rin A F PG F A

12 β β β SDS PAGE B kda a kda c β kda b PG F F F IgE kda F F RIN rin F

13 rin F rin rin Cryj PG A

14 rin rin rin RIN/rin F rin RIN/rin Kitagawa, M., Moriyama, T., Ito, H., Ozasa, S., Adachi, A., Yasuda, J., Ookura, T., Inakuma, T., Kasumi, T., Ishiguro, Y. and Y., I., Reduction of allergic proteins by the effect of the ripening inhibitor (rin) mutant gene in F1 hybrid of the rin mutant tomato. Biosci Biotechnol Biochem, 70, (2006).

15 Kitagawa, M., Ito, H., Shiina, T., Nakamura, N., Inakuma, T., Kasumi, T., Ishiguro, Y., Yabe, K. and Ito, Y., Characterization of tomato fruit ripening and analysis of gene expression in F1 hybrids of the ripening inhibitor (rin) mutant. Physiol Plant, 123, (2005). Kitagawa,M.,Nakamura,N.,Usuda,U.,Shiina,T.,Ito,H.,Yasuda,J., Inakuma, T., Ishiguro, Y., Kasumi, T. and Y., I., Ethylene biosynthesis regulation in tomato fruit of F1 hybrid of the ripening inhibitor (rin) mutant. Biosci Biotechnol Biochem, 70, (2006). Smith,C.J.S.,Watson,C.F.S.,Ray,J.,Bird,C.R.,Morris,P.C.,Schuch, W. and Grierson, D., Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature, 334, (1988). Schuch, W., Kanczler, J., Robertson, D., Hobson, G., Tucker, G., Grierson, D., Bright, S. and Bird, C., Fruit quality characteristics of transgenic tomato fruit with altered polygalacturonase activity. HortScience, 26, (1991). Smith, D. L., Abbott, J. A. and Gross, K. C., Down regulation of tomato beta galactosidase 4 results in decreased fruit softening. Plant Physiol, 129, (2002). Brummell, D. A., Harpster, M. H., Civello, P. M., Palys, J. M., Bennett, A. B. and Dunsmuir, P., Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism during ripening. Plant Cell, 11, (1999). Bleumink, E., Berrens, L. and Young, E., Studies on the atopic allergen in ripe tomato fruits. II. Further chemical characterization of the purified allergen. Int Arch Allergy Appl Immunol, 31,25 37 (1967). Hamilton, A. J., Lycett, G. W. and Grierson, D., Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature, 346, (1990). Oeller, P. W., Lu, M. W., Taylor, L. P., Pike, D. A. and Theologis, A., Reversible inhibition of tomato fruit senescence by antisense RNA. Science, 254, (1991). Wilkinson,J.Q.,Lanahan,M.B.,Yen,H.C.,Giovannoni,J.J.andKlee, H. J., An ethylene inducible component of signal transduction encoded by never ripe. Science, 270, (1995). Vrebalov, J., Ruezinsky, D., Padmanabhan, V., White, R., Medrano, D., Drake, R., Schuch, W. and Giovannoni, J., A MADS box gene necessary for fruit ripening at the tomato ripening inhibitor (rin) locus. Science, 296,

16 (2002). Tigchelaar, E. C., McGlasson, W. B. and Buescher, R. W., Genetic regulation of tomato fruit ripening. HortScience, 13, (1978). Barry,C.S.,Llop Tous,M.I.andGrierson,D.,Theregulationof1 aminocyclopropane 1 carboxylic acid synthase gene expression during the transition from system 1 tosystem 2 ethylene synthesis in tomato. Plant Physiol, 123, (2000). Kondo, Y., Urisu, A. and Tokuda, R., Identification and characterization of the allergens in the tomato fruit by immunoblotting. Int Arch Allergy Immunol, 126, (2001). Westphal, S., Kolarich, D., Foetisch, K., Lauer, I., Altmann, F., Conti, A., Crespo, J. F., Rodriguez, J., Enrique, E., Vieths, S. and Scheurer, S., Molecular characterization and allergenic activity of Lyce 2 (beta fructofuranosidase), a glycosylated allergen of tomato. Eur J Biochem, 270, (2003). Takahashi, K., Banba, H., Kikuchi, A., Ito, M. and Nakamura, S., An induced mutant line lacking the a subunit of b conglycinin in soybean [Glycine max (L.) Merrill]. Breeding Science, 44, (1994). Tada, Y., Nakase, M., Adachi, T., Nakamura, R., Shimada, H., Takahashi, M., Fujimura, T. and Matsuda, T., Reduction of kda allergenic proteins in transgenic rice plants by antisense gene. FEBS Lett, 391, (1996). Dodo, H., Konan, K. and Viquez, O., A genetic engineering strategy to eliminate peanut allergy. Curr Allergy Asthma Rep, 5, (2005). oral allergy syndrome Namba, M., Kurose, M., Torigoe, K., Hino, K., Taniguchi, Y., Fukuda, S., Usui, M. and Kurimoto, M., Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen. FEBS Lett, 353, (1994).

24 10 QOL 6) 7) 2 8,9) Cry j 1 10,11) Cry j 2 12,13) Cha o 1 14) Cha o 2 15) Cry j 1 Cha o 1 80% Cry j 2 Cha o 2 74% T B Cry j 1 Cha o 1 IL-5 1) 1,16)

24 10 QOL 6) 7) 2 8,9) Cry j 1 10,11) Cry j 2 12,13) Cha o 1 14) Cha o 2 15) Cry j 1 Cha o 1 80% Cry j 2 Cha o 2 74% T B Cry j 1 Cha o 1 IL-5 1) 1,16) JJIAO 36 1 : 23 28, 2018 総説 1,2 1 2 Cha o 3 ImmunoCAP 2 87.5% Cha o 3 IgE Cha o 3 IgE Cha o 1 ImmunoCAP Cha o 3 IL-5 Cha o 3 Cha o 3 IgE, Immunoglobulin E; PAS, Periodic acid-schiff; SDS-PAGE, Sodium

食糧 その科学と技術 No.45( )

食糧　その科学と技術　No.45( )