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1 文部科学省科学研究費補助金新学術領域研究修飾シグナル病第二回公開シンポジウム修飾シグナル病学術領域の新展開抄録集プログラム 2012年1月28日土 13:00 17:30 東京大学医科学研究所 1号館1F講堂領域代表者井上純一郎

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3 13:10-13:40 NF-κB NF-κB IκB kinase (IKK) NF-κB IκB IκB NF-κB IKK IKK IκB IKK NF-κB IKK IKK T I (HTLV-I) Tax IKK NEMO IKK Tax IKK NIK NIK NF-κB TRAF6 RIP1K A20 IKK NF-κB HTLV-I IKK A20 A20 B A20 NF-κB 1. Saitoh, Y. et al., Lung Cancer, 70, , Saitoh, Y. et al., Blood, 111, , Nonaka, M. et al., Oncogene, 24, , Saitoh, T. et al., J. Immunol., 174, ,

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5 13:40-14:10 1) SILAC Stable Isotope Labeling by Amino acids in Cell culture EGF Src 2) MCF-7 3) 1. Oyama M, Kozuka-Hata H, Suzuki Y, Semba K, Yamamoto T and Sugano S. Diversity of translation start sites may define increased complexity of the human short ORFeome. Mol Cell Proteomics, 6: (2007). 2. Oyama M, Kozuka-Hata H, Tasaki S, Semba K, Hattori S, Sugano S, Inoue J and Yamamoto T. Temporal perturbation of tyrosine-phosphoproteome dynamics reveals the system-wide regulatory networks. Mol Cell Proteomics, 8: (2009). 3. Oyama M, Nagashima T, Suzuki T, Kozuka-Hata H, Yumoto N, Shiraishi Y, Ikeda K, Kuroki Y, Gotoh N, Ishida T, Inoue S, Kitano H and Okada-Hatakeyama M. Integrated quantitative analysis of the phosphoproteome and transcriptome in tamoxifen-resistant breast cancer. J Biol Chem, 286: (2011)

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7 14:10-14:40 1 (IGF-1) IGF-1 IGF-1 PI3K Akt GSK-3β nebulin (Neb) C Z GSK-3β Neb SH3 N-WASP Pro-rich N-WASP Z Neb N-WASP WH2 Z Neb 1 µm 1 µm Neb C NEB Neb N-WASP Neb 0.15 µm nebulette (Nebt) 1 µm N-WASP Nebt C Z Nebt N-WASP Nebt 0.15 µm Nebt N 0.85 µm 1 µm NEBT Nebt 1. Takano, K. et al. Science 330: (2010). 2. Watanabe-Takano, H. et al. Exp. Cell Res. 316: (2010). 3. Yokoyama, T. et al. J. Cell Biol. 177: (2007). 4. Endo, T. Regen. Med. 2: (2007). 5. Sun, P. et al. Genes Cells 11: (2006)

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9 14:40-15:10 E-box DNA 24 CACGTG E-box bhlh-pas CLOCK BMAL1 E-box Per Cry PER CRY CLOCK-BMAL1 CLCOK bhlh E-box bipartite (3) CRY (2) SREBP E4bp4 (1) TGF-β ALK5 SMAD2/3 Dec1 (4) 1. Hatori et al. Proc. Natl. Acad. Sci. USA. 108, (2011). 2. Kurabayashi, N. et al. Mol. Cell Biol. 30, (2010) 3. Yoshitane, H. et al. Mol. Cell Biol. 29, (2009) 4. Kon N. et al. Nat. Cell Biol. 10, (2008)

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11 15:30-16:10 GTP FRET FRET FRET 1. K. Aoki, M. Yamada, K. Kunida, S. Yasuda, and M. Matsuda. Processive phosphorylation of ERK MAP kinase in mammalian cells. Proc Natl Acad Sci U S A 108: , N. Komatsu, K. Aoki, M. Yamada, H. Yukinaga, Y. Fujita, Y. Kamioka, and M. Matsuda. Development of an optimized backbone of FRET biosensors for kinases and GTPases. Mol Biol Cell 22 (23): , E. Hirata, H. Yukinaga, Y. Kamioka, Y. Arakawa, S. Miyamoto, T. Okada, E. Sahai, and M. Matsuda. In vivo fluorescence resonance energy transfer imaging reveals differential activation of Rho-family GTPases in glioblastoma cell invasion. J Cell Sci, in Press

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13 16:10-16: DNA DNA 2 rule of thumb nm nm nm nm 1 A. Kusumi et al. Traffic 5, (2004). A. Kusumi et al. Annu. Rev. Biophys. Biomol. Struct. 34, (2005). A. Kusumi et al. Trends Biochem. Sci. 36, (2011) icems

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15 16:50-17: P ph Phos-tag Phos-tag 1 Phos-tag Kinoshita, E. et al. Mol. Cell. Proteomics 5, (2006) Kinoshita, E. and Kinoshita-Kikuta, E. Proteomics 11, (2011) Kinoshita, E. et al. Proteomics (in press) [doi: /pmic ]

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17 17:40-16 NLK Lef1 Wnt/ NLK positively regulates Wnt/betacatenin signaling byphosphorylating Lef1 in neural progenitor cells The Nestin cell lineage is responsible for the lethal phenotypes of Girdin straight knockout mice. DYRK2 c-jun/c-myc G1/S In silico Spatio-temporal Simulation of NF-κB Oscillation Oscillationtion of non-canonical NF-κB pathway Kinetic analysis of persistent NF-κB activation during PMA-induced differentiation of human monocytic THP-1 cells Ubiquitin-editing enzyme A20 contributes to the survival of cancer cells Akt/PKB Plk1 ph Phos-tag SDS- PAGE A20 zinc finger7 LUBAC NF-κB PGAM5 A20 zinc finger7 NASH) Pin1 PAR14 HOILI-1L VCP DJ-1 p53 MAPK Rab6A Stress-inducible caspase substrate TRB3 promotes nuclear translocation of procaspase-3 CCP1 EGFR Ser/Thr MAPKKK A role of Pin1 in neural differentiation TRIM Terf Dok-7 Critical roles of a metalloendopeptidase nardilysin in cold-induced adaptive thermogenesis CRY2 Activation of PKR by ubiquitin-like molecule ISG15 modification (ISGylation). FGF

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