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1 P2 3 P P5 10 P11 P12 P13 P14 PCR P15 17 PCR P18 20 P21 23 P24 P25 26 P27 31! 17

2 EVOS XL Core 627, ,000 LED 1 EVOS XL Core Imaging System AMEX1000 EVOS 4X Objective, achromat, LWD, phase-contrast AMEP4632 EVOS 10X Objective, achromat, LWD, phase-contrast AMEP4633 EVOS Condenser Slider, Diffusion AMEPDFS1 XY XYInvitrogen EVOS XL Core Attachable Mechanical Stage AMEP ,000XY RC2T42 NEW Invitrogen EVOS M5000! Z- thermofisher.com/evosm thermofisher.com/jp-dls

3 CytoVista 30% OFF 3D 3Invitrogen CytoVista Tissue and 3D Culture Clearing Reagents 30%OFF 3 8 mm 24~48 3 CytoVista H&E DAB CytoVista HepG2 Ki67 Invitrogen Click-iT EdU CytoVista 1 8 mm : CytoVista 3D Cell Culture Clearing Reagent CytoVista Tissue Clearing Reagent 48 2 CytoVista Tissue Clearing Kit CytoVista 3D Cell Culture Clearing/Staining Kit CytoVista Tissue Clearing/ Staining Kit mm µm µm D cell culture models >200 µm Invitrogen CytoVista Tissue Clearing Reagent Invitrogen CytoVista Tissue Clearing Enhancer or CytoVista Tissue Clearing Reagent 30 ml V ,300 38,700 CytoVista Tissue Clearing Enhancer 30 ml V ,400 10,000 CytoVista 3D Cell Culture Clearing Reagent 30 ml V ,600 48,000 CytoVista Tissue Clearing Kit 10 ml V ,000 16,800 Tissue Clearing Reagent Tissue Clearing Enhancer 30 ml V ,900 44, ml V ,100 92,400 CytoVista Tissue Clearing / Staining Kit Tissue Clearing Reagent, 30 ml Tissue Clearing Enhancer, 30 ml Antibody Dilution Buffer, 30 ml Blocking Buffer, 30 ml Antibody Penetration Buffer, 30 ml 10X Wash Buffer, 70 ml Permeabilization Buffer, 30 ml CytoVista 3D Cell Culture Clearing / Staining Kit 3D Cell Culture Clearing Reagent, 30 ml Antibody Dilution Buffer, 30 ml Blocking Buffer, 30 ml Antibody Penetration Buffer, 30 ml 10X Wash Buffer, 70 ml Invitrogen CytoVista Tissue Clearing/Staining Kit Invitrogen CytoVista 3D Cell Culture Clearing/Staining Kit RCCS22 1 kit V ,400 72,300 1 kit V ,500 63,300 thermofisher.com/jp-dls 3

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5 Gibco Cell Culture Produ ct Gibco Cell Culture Produ ct 販売代理店様へ フリガナ お名前 ご勤務先名称 ご所属先詳細 ( 部署 / 研究室名など ) ご勤務先住所 アドレス 当選者の発表は 発送をもってかえさせていただきます プレゼントの発送は順次お届けする予定です FAX や電子メールでのお申し込みは無効となります 必ず郵送にてお送りください お一人様 1 回限りのご応募とさせていただきます 応募先 Gibco Cell Culture Product Gibco Cell C ulture Product Gibco 特製ポーチプレゼント Gibco Cell Culture Product Gibco Cell Culture Product Gibco Cel l Culture Product Gibco Cell Culture Product 応募締切 : 2017 年 12 月 22 日 ( 金 ) 当日消印有効 Gibco Cell Culture Product Gibco Cell Culture Product 本キャンペーンのプレゼント応募は 培地製品のキャップラベルシールのみが対象となります 培地以外の製品 ( 血清 サプリメント 解離用試薬 PBS 等の試薬 ) は対象外となりますのであらかじめご了承ください プレゼントは 下記お申し込みのお客様宛に直接発送させていただきます 電話番号 FAX 番号 部署 / 学部名 ご記入いただいた個人情報は ライフテクノロジーズジャパン株式会社の規定に従い厳正に管理いたします 理由なく第三者に開示することはございません 個人情報保護方針 の詳細はこちらをご覧ください 東京都港区芝浦 住友不動産三田ツインビル東館サーモフィッシャーサイエンティフィックライフテクノロジーズジャパン株式会社マーケティング部 Gibco 係 Gibco 2019 Gibco Gibco 300 Gibco Gibco 10 Gibco A4 1 2 応募 下記 URL よりお申し込み用紙をダウンロードし プリントアウト キャップラベルをお申し込み用紙に貼って ラベル見本 3 郵送にてお送りください 1 6 Gibco キャップラベルコレクション 2017 用台紙 thermofisher.com/jp-clc PBS FAX 1 Gibco 100 Gibco Life of Cells thermofisher.com/cellculturebasics thermofisher.com/jp-dls 5

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11 Neon Transfection System Invitrogen Neon Neon Transfection System Neon DNA RNA Jurkat 細胞へのプラスミド導入結果 Neon システムを用いて Jurkat 細胞へ EGFP 遺伝子を導入後 24 時間でレポーター遺伝子の発現を観察した (A) A 位相差顕微鏡での細胞像 (B) B 蛍光顕微鏡での細胞像 ゲノム編集にも幅広く活用できます Neon システムは 一般的なトランスフェクション実験だけでなく 最近急速に広がっているゲノム編集技術においても大変有用なツールです リポフェクション試薬では導入が難しい細胞を用いたゲノム編集実験において さまざまなフォーマットの CRISPR の導入に活用いただけます ゲノム編集用トランスフェクションの選択 画期的なピペットチップ型電極 トランスフェクション 試薬および機器 Lipofectamine CRISPRMAX Lipofectamine 3000 CRISPR DNA CRISPR delivery methods CRISPR mrna CRISPR protein Lenti-Viral production 10 µl という極微量でのエレクトロポレーションが可能 従来のキュベットタイプと比べて Cas9 タンパク質のコストを大幅に削減できます Neon システム Lipofectamine MessengerMAX さまざまな種類の細胞で Neon を用いた Cas9 タンパク質によるゲノム編集の実績があります 参考論文 Biotechnology Letters, 2016 Jun ; 38(6): Neon Neon Transfection System Starter Pack 1 1 MPK5000S 1,164, ,000 Neon Transfection System 2 1 MPK5000 1,000, ,000 Neon Neon Transfection System Kit 100 µl 1 kit Neon 96 MPK ,400 1 kit Neon 25 MPK ,200 Neon Transfection System Kit 10 µl 1 kit Neon 96 MPK ,400 1 kit Neon 25 MPK ,200 1 Neon Transfection System Starter Pack MPK5000S Neon Transfection System MPK5000 Neon Transfection Kit MPK10096 MPK Neon Transfection System MPK5000 Neon Neon Pipette MPP100 Neon Pipette Station MPS100 RCAH28 thermofisher.com/jp-dls 11

12 Invitrogen LentiArray CRISPR Library Invitrogen LentiArray CRISPR Library 2 grna 4 grna 1 x 10 8 TU / ml 100 µl 50 µl 2 sgrna 発現用フォーマット LTR psi cppt PRE plenticrispr-efs-puro sgrna Scaffold Pol lll term U6 FES Puro Unique sgrna U6プロモーターと特異的 sgrnaを接続 EF-1aプロモーターとPuromycin 耐性遺伝子 (Puro) を接続 WPRE LTR Human Kinase CRISPR Library 100 µl 2,980,000 LentiArray Human Kinase CRISPR Library 822 A31931 A32167 LentiArray Human GPCR CRISPR Library 446 A31947 A32183 LentiArray Human Epigenetics CRISPR Library 396 A31934 A32170 LentiArray Human Phosphatase CRISPR Library 288 A31932 A32168 LentiArray Human DNA Damage Response CRISPR Library 516 A31946 A32182 LentiArray Human Cell Surface CRISPR Library 778 A31943 A32179 LentiArray Human Cancer Biology CRISPR Library 510 A31933 A32169 LentiArray Human Ubiquitin CRISPR Library 943 A31935 A32171 LentiArray Human Apoptosis CRISPR Library 904 A31940 A32176 LentiArray Human Tumor Suppressor CRISPR Library 716 A31945 A32181 LentiArray Human Transcription Factors CRISPR Library 1,817 A31938 A32174 LentiArray Human Cell Cycle CRISPR Library 1,444 A31936 A32172 LentiArray Human Drug Transporter CRISPR Library 98 A31941 A32177 LentiArray Human Ion Channel CRISPR Library 328 A31942 A32178 LentiArray Human Membrane Trafficking CRISPR Library 141 A31937 A32173 LentiArray Human Nuclear Hormone Receptors CRISPR Library 47 A31939 A32175 LentiArray Human Protease CRISPR Library 475 A31944 A32180 LentiArray Human Druggable CRISPR Library 10,132 A31948 A32184 LentiArray Human Whole Genome CRISPR Library 18,453 A31949 A32185 CRISPR CRISPR sirna TEL: thermofisher.com/lentiarraylibraries 12 thermofisher.com/jp-dls

13 DNA Qubit 4 Fluorometer 1 DNA RNA1 Invitrogen Qubit Invitrogen Qubit 4 Fluorometer 1 Qubit NGS PCR 2,000? dsdna PCR Qubit Result in ng/µl Quant-iT dsdna BR Assay Quant-iT RNA BR Assay 分光光度計 A 分光光度計 B 1 1 dsdna ssdna RNA :0 10:5 10:10 10:20 10:30 10:40 10:50 10:75 10:100 Qubit Fluorometer DNA:RNA ratio(ng / µl) ng / µl ribosomal E. coli RNA 10 ng / µl lambda DNA Qubit Fluorometer DNARNA Qubit 4 Fluorometer thermofisher.com/qubit Qubit 4 Fluorometer 1 Q ,000 Qubit Assay Tubes 500 tubes Q ,600 Qubit 1X dsdna HS Assay Kit 500 assays Q , assays Q ,800 Qubit dsdna BR Assay Kit 100 assays Q ,000 Qubit RNA HS Assay Kit 100 assays Q ,000 Qubit RNA XR Assay Kit 100 assays Q ,300 Qubit RNA IQ Assay Kit 275 assays Q , assays Q ,800 Qubit microrna Assay Kit 100 assays Q ,000 Qubit Protein Assay Kit 100 assays Q ,000 MLHC27 thermofisher.com/jp-dls 13

14 Ion GeneStudio S5 Plus Ion GeneStudio S5 Plus Ion 550 Chip Ion GeneStudio S5 19 Ion 540 Chip Ion GeneStudio S5 Plus 10 Ion 550 Chip Gb Ion GeneStudio S5 Plus GSPLU-UPCP 12,300,000 7,500,000 Ion Torrent 30% OFF Ion AmpliSeq Library Kit Plus , ,700 Ion AmpliSeq Library Kit ,000 87, ,200, , ,640,000 2,548,000 Ion AmpliSeq Kit for Chef DL8 32 A , ,300 Ion Total RNA-Seq Kit v , , , ,500 Ion Plus Fragment Library Kit ,000 54,600 Ion Xpress Plus Fragment Library Kit ,000 74,200 RCBB35 14 thermofisher.com/jp-dls

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16 PCR PCR PCR / PCR 96 Thermo Scientific PCR PCR DNA RNase DNase PCR black lettering AB0600L AB0700L AB0800L AB0800WL AB1400L AB1400WL PCR AB0600W AB0800W AB0900W AB1400W AB0600 AB0700 AB0800 AB0900 AB1400 H1 H12 H1 H1 A12 PCR Plate, 96-well, non-skirted AB0600 PCR Plate, 96-well, low profile, non-skirted AB0700 PCR Plate, 96-well, low profile, skirted AB0800 PCR Plate, 96-well, segmented, semi-skirted AB0900 PCR Plate, 96-well, semi-skirted, flat deck AB ml H1 0.2 ml H ml H1 ANSI Low profile 0.3 ml H ml A12 PCR Plate, 96-well, non-skirted 25 AB ,100 12,600 PCR Plate, 96-well, non-skirted, black lettering 25 AB0600L 18,600 13,000 PCR Plate, 96-well, non-skirted, white 25 AB0600W 18,600 13,000 PCR Plate, 96-well, low profile, non-skirted 25 AB ,100 10,500 PCR Plate, 96-well, low profile, non-skirted, black lettering 25 AB0700L 20,600 14,400 PCR Plate, 96-well, low profile, skirted 25 AB ,200 13,400 PCR Plate, 96-well, low profile, skirted, black lettering 25 AB0800L 20,500 14,300 PCR Plate, 96-well, low profile, skirted, white 25 AB0800W 18,800 13,100 PCR Plate, 96-well, low profile, skirted, black lettering, white 25 AB0800WL 21,900 15,300 PCR Plate, 96-well, segmented, semi-skirted 25 AB ,500 9,400 PCR Plate, 96-well, segmented, semi-skirted, white 25 AB0900W 18,200 12,700 PCR Plate, 96-well, semi-skirted, flat deck 25 AB ,200 10,600 PCR Plate, 96-well, semi-skirted, flat deck, black lettering 25 AB1400L 19,600 13,700 PCR Plate, 96-well, semi-skirted, flat deck, white 25 AB1400W 18,100 12,600 PCR Plate, 96-well, semi-skirted, flat deck, black lettering, white 25 AB1400WL 21,900 15,300 Adhesive PCR Plate Seals 100 AB ,500 14,300 Adhesive PCR Plate Foils 100 AB ,100 11,900 ABsolute qpcr Plate Seals 50 AB ,300 11, thermofisher.com/jp-dls

17 30% OFF Phusion High-Fidelity DNA Polymerase Thermo Scientific Phusion High-Fidelity DNA Polymerase Pyrococcus DNA PCR DNA DNA DNA 1 Taq DNA DNA s/kb 15 s/ kb 1 20 kb DNA F555S F555L 1 Phusion Flash High-Fidelity PCR Master Mix Phusion High-Fidelity DNA Polymerase 2 U / L 100 units F530S 21,600 15, units F530L 94,800 66,300 Phusion Flash High-Fidelity PCR Master Mix F548S 17,100 11, F548L 83,400 58,300 Phusion Hot Start II High-Fidelity DNA Polymerase 2 U / L 100 units F549S 25,500 17, units F549L 105,100 73,500 Phusion U Hot Start DNA Polymerase 2 U / L 100 units F555S 31,900 22, units F555L 128,300 89, L O'RangeRuler DNA Ladder 10 6,000 bp Thermo Scientific O RangeRuler DNA Ladder PCR DNA DNA DNA O'RangeRuler 5 bp DNA Ladder SM ,400 20,500 O'RangeRuler 10 bp DNA Ladder 0.1 µg / µl 50 µg SM ,000 18,900 O'RangeRuler 20 bp DNA Ladder SM ,200 16,900 O'RangeRuler 50 bp DNA Ladder SM ,900 10,400 O'RangeRuler 100 bp DNA Ladder SM ,200 9,900 O'RangeRuler 200 bp DNA Ladder 0.05 µg / µl 25 µg 100 SM ,200 9,900 O'RangeRuler 500 bp DNA Ladder SM ,200 9,900 O'RangeRuler bp DNA Ladder SM ,200 9,900 RCTV24 thermofisher.com/jp-dls 17

18 PCR PowerUp SYBR Green Master Mix 20% OFF Applied Biosystems PowerUp SYBR Green Master Mix Dual-Rock Taq PowerUP SYBR Green Master Mix PowerUp SYBR Green Master Mix PowerUp SYBR Green Master Mix 28 DNA Nuclease Free Water No Template Control 96 n=3 PowerUp SYBR Green Master Mix No Template Control Ct 35 PowerUp SYBR Green Master Mix Ct Ct Ct PowerUp SYBR Green Master Mix 5 ml A ,800 29, ml A ,500 32, ml A ,400 57, ml A ,500 62, ml A , , ml A , , ml A , ,800 RCAT22 18 thermofisher.com/jp-dls

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