MicroRNA_brochure_1205b.indd
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- れれ ひろき
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1 microrna製品カタログ microrna製品カタログ having a big impact on biology MicroRNA_brochure_1205b.indd 1 12/12/05 16:36
![MicroRNA RNA mirna RNA [1*] mirna mirna [2*] mirna RNA 17](/docs-images/100/145775072/images/2-0.jpg "24 mirna mirnasmall RNA mirna mirna mirna 2 *Reference 26")
2 MicroRNA RNA mirna RNA [1*] mirna mirna [2*] mirna RNA mirna mirnasmall RNA mirna mirna mirna 2 *Reference 26
3 Contents 4 small-rna 8 microrna 13 mirna 18 microrna 23 microrna 26 3
4 small-rna small RNA 2 10,000small RNA mirbase [3] smallrna Ion PGM RNA small RNA Ion Ion PGM small RNA Ion Total RNA-Seq v2 1 ng small RNAsmall RNAcDNA Ion Total RNA-Seq v2 Ambion US Patent: ' 5' Ion OneTouch 3 Ion PGM 1 Torrent Suite 1 small RNAsmall RNAcDNA cdna small RNA cdna small RNA small RNA TaqMan 5'nuclease small-rna 4
5 Ion small RNA-Seq small RNA Ion RNA-Seq kit v2 Ion 314 Ion 316 mirna 3 Ion 314 3Ion GeneChip mirna 3 TaqMan Array MicroRNA 2 mirnaion PGM 1miRNA Ion Ion RNA-Seq LogRatio 2 1. Ion small RNA-Seq mirnaion PGM mirbasemirna TaqMan C t < 40 3 mirna2 mirna Ion PGM mirna Ion PGM mirna -3p -5p leading star * 2 hsa-mir-126 mirna Ion PGM 5' 3'isomir isomir 2. Ion PGM mirna -3p -5p TaqMan MicroRNA Ct log2mapped Ion PGM log2 hsa-mir-126 hsa-mir-126* TaqMan Not detected Ion PGM Total assays Intersection assays Intersection detected TaqMan GeneChip Array Ion Ion log 2 Ion PGM log 2 (/ ) PGM 316 PGM 314 Array 2. Ion small RNA-Seq Ion 316 Ion 314 ChipsLogRatioGeneChip mirna Ordering information Ion PGM Ion PGM Ion Torrent Ion One Touch 2.0 Duo VWR Galaxy MiniStar 16 PGM-410M 2 PGM-400M 1 12,200,000 11,400,000 5
6 Ion Total RNA-Seqv2 RiboMinus Eukaryote v2 Ion Total RNA-Seq v2small RNA small RNA small RNA 100ng RNA5ng mirna 1ng mirna small RNA 1 small RNA 16 RNA RiboMinus Eukaryote v2rna RNA RNA Total RNA18S 28S 5S 5.8S rrna 95rRNA 5rRNA/ PolyA+ mrnapolya+ RNARNA RNART-PCR rrna 95 1 RiboMinus Eukaryote System v2 1 5 g RNA Low Input RiboMinus Eukaryote System v2 100ng-1 g RNA RNA 30 1 v21 RNA Ion Total RNA-Seq Step 1 Step 2 Step 3 Step 4 Hybridized RiboMinus probe Biotin Non rrnas Bead Streptavidin Biotin Non rrnas Magnet rrna-depleted RNA RNA RNA / 30 Ion Total RNA-Seq v2 Ordering information Ion Total RNA-Seq kit v2 12Rxn ,000 Ion Total RNA-Seq kit v2 48Rxn ,000 Ion Xpress RNA-Seq Bc 01-16kit ,000 RiboMinus Eukaryote System v2 12 A ,000 Low Input RiboMinus Eukaryote System v2 12 A ,000 6
7 ERCC RNA Spike-In ERCC RNA Spike-In NIST National Institute of Standards & TechnologyERCC External RNA Control ConsortiumRNA 92RNA RNA polyasolid Total RNArRNA polyA RNA 10 6 PCR small RNA total RNA Spike-In rrna polya RNA RNA Spike-In RNA Spike-In 1 Spike-In 2 RNA PCR TaqMan Assay 1e+9 ERCC Mix 1 molecules/100ng mrna 1e+8 1e+7 1e+6 1e+5 1e+4 1e+3 1e+2 1e+2 1e+3 1e+4 1e+5 1e+6 1e+7 1e+8 1e+9 ERCC Mix 2 molecules/100ng mrna 3. ERCC ExFold RNA Spike-In ExFold RNA Spike-In polyA RNA ERCC RNA Spike-In 1 ExFold Spike-In Ordering information ERCC ExFold RNA Spike-In 10 µl ,000 ERCC RNA Spike-In 10 µl ,000 7
8 microrna TaqMan 1 microrna mirna mirna mirna mirna mirna TaqMan OpenArray MicroRNA Panel TaqMan Array Micro RNA CardMegaplex Primer Pools mirna 48 mirna 100 ng RNATaqMan PCR RT TaqMan OpenArray MicroRNA TaqMan Array MicroRNA1 100 ng RNA 4 5miRNA mirbase mirna TaqMan Array MicroRNA Card 377miRNA1 A B 377miRNA 2 TaqMan OpenArray MicroRNA Panel754miRNA 2 Megaplex RT Primers 377miRNA Applied Biosystems PCR Megaplex PreAmp Primer Pools mirna PCR TaqMan Universal PCR Master Mix II TaqMan OpenArray MicroRNA Panel AccuFill 3754TaqMan MicroRNA AssaysTaqMan Array MicroRNA CardCard TaqMan MicroRNA Card A B 2 377miRNA Assay 1 cdna cdna 2 (RT) PCR 6 () mir Vana TM mirna Isolation Kit TaqMan ABC Purification Kit mirna Megaplex TM RT Primers TaqMan MicroRNA Reverse Transcription Kit Megaplex TM PreAmp Primers & TaqMan PreAmp Master Mix TaqMan Array Card or OpenArray Plate QuantStudio 12K Flex, ViiA HT Fast PCR RealTime StatMiner Software DataAssist Software Total RNA xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx xxxxxxxx 30 min 150 min 90 min 10 min 60 min 4. TaqMan OpenArray MicroRNA Panel TaqMan Array MicroRNA Cards mirna TaqMan OpenArray MicroRNA Panels Megaplex TM PreAmp Primer Poolspre-amplification pre-amplification 4.25 TaqMan Array MicroRNA CardsMegaplex TM PreAmp Primer Poolspre-amplification
9 Megaplex Megaplex Primer Pools TaqMan OpenArray MicroRNA PanelTaqMan Array MicroRNA CardMegaplex Primer PoolsmiRBase mirna mirna Megaplex RT Primer Pools mirna Megaplex RT Primer Pools mirnart cdnataqman MicroRNA Assays Megaplex PreAmp Primer Pools TaqMan Array MicroRNA Cards Megaplex PreAmp Primer Pools mirna Megaplex PreAmp Primer Pools1 ng RNAmiRNA 5 mirna TaqMan MicroRNA TaqMan MicroRNA ArraysmicroRNA TaqMan MicroRNA Assay Megaplex Primer Pools TaqMan OpenArray MicroRNA Panels TaqMan OpenArray MicroRNA Panels 1 3 mirna Megaplex RT Primer PoolsMegaplex PreAmp Primer Pools TaqMan Universal PCR Master Mix II TaqMan Arrays MicroRNA Cards TaqMan OpenArray Panel log 4 log RNA PCR logmirna 1 mirna mirna TaqMan 4 RT mirna RNA mirna 1 g 100 ng in silico mirna 1 1,000 ng in silico Megaplex Primer Pools TaqMan Array Cards Megaplex Primer Pools Megaplex Human A Pool Megaplex Human B Pool % % ntc 0.1 fm 1 fm 10 fm 100 fm 1 pm 10 pm 100 pm 0 ntc 0.1 fm 1 fm 10 fm 100 fm 1 pm 10 pm 100 pm RT PreAmp RT PreAmp 5. Megaplex Primer Pools TaqMan Array MicroRNA CardsmiRNA TaqMan MicroRNA Array A Card B Card CardmiRNA 10 pmtotal RNA 10ng/ L 6 log 4 logmegaplex NTC 9
10 Ordering information microrna mirvana mirna Isolation Kit TaqMan mirna ABC Purification Kit microrna RT TaqMan MicroRNA RT Kit TaqMan MicroRNA RT KitTaqMan MicroRNA Assays Megaplex Primer PoolsTaqMan MicroRNA Assays RT TaqMan MicroRNA RT Kit ,000 TaqMan MicroRNA RT Kit ,000 Megaplex Primer Pools Megaplex RT Primers stem-loopedtaqman MicroRNA Arrays mirna mirna RNA Megaplex RT Primers Pool for Human Megaplex RT Primers, Human Pool A ,000 Megaplex RT Primers, Human Pool B ,000 Megaplex RT Primers, Human Pool Set ,000 RT Primers Pools A&B ( & ) Megaplex RT Primers, Human Pool B v ,000 Megaplex RT Primers, Human Pool Set v3.0 RT Primers Pools A&B ( & ) ,000 Megaplex RT Primers Pool for Rodent Megaplex RT Primers, Rodent Pool A ,000 Megaplex RT Primers, Rodent Pool B ,000 Megaplex RT Primers, Rodent Pool Set ,000 RT Primers Pools A&B ( & ) Megaplex RT Primers, Rodent Pool B v ,000 Megaplex RT Primers, Rodent Pool Set v3.0 RT Primers Pools A&B( & ) ,000 Megaplex Primer Pools Pool APool BTaqMan MicroRNA Arrays A B PreAmpcDNA Megaplex PreAmp Primers1ng RNA Megaplex PreAmp Primers Pool for Human Megaplex PreAmp Primers, Human Pool A ,000 PreAmp Megaplex PreAmp Primers, Human Pool B PreAmp Megaplex PreAmp Primers, Human Pool Set PreAmp Primers Pools A&B ( & ) Megaplex PreAmp Primers, Human Pool B v3.0 PreAmp Megaplex PreAmp Primers, Human Pool Set v3.0 PreAmp Primers Pools A&B ( & ) , , , ,000 10
11 Megaplex RT and PreAmp Primers Package for Human Megaplex Primer Pools, Human Pools A ,000 RT Primers( ) + PreAmp Primers( ) Megaplex Primer Pools, Human Pools B ,000 RT Primers( ) + PreAmp Primers( ) Megaplex Primer Pools, Human Pools Set ,000 RT Primers Pools A&B ( (= & )) PreAmp Primers Pools A&B ( (= & )) Megaplex Primer Pools, Human Pools B v ,000 RT Primers( ) + PreAmp Primers( ) Megaplex Primer Pools, Human Pools Set v3.0 RT Primers Pools A&B ( (= & )) PreAmp Primers Pools A&B ( (= & )) ,000 Megaplex PreAmp Primers Pool for Rodent Megaplex PreAmp Primers, Rodent Pool A ,000 PreAmp Megaplex PreAmp Primers, Rodent Pool B ,000 PreAmp Megaplex PreAmp Primers, Rodent Pool Set ,000 PreAmp Primers Pools A&B ( & ) Megaplex PreAmp Primers, Rodent Pool B v ,000 PreAmp Megaplex PreAmp Primers, Rodent Pool Set v3.0 PreAmp Primers Pools A&B( & ) ,000 Megaplex RT and PreAmp Primers Package for Rodent Megaplex Primer Pools, Rodent Pools A ,000 RT Primers ( ) + PreAmp Primers ( ) Megaplex Primer Pools, Rodent Pools B ,000 RT Primers ( ) + PreAmp Primers ( ) Megaplex Primer Pools, Rodent Pools Set ,000 RT Primers Pools A&B ( (= & )) PreAmp Primers Pools A&B ( (= & )) Megaplex Primer Pools, Rodent Pools B v ,000 RT Primers( ) + PreAmp Primers( ) Megaplex Primer Pools, Rodent Pools Set v3.0 RT Primers Pools A&B( (= & )) PreAmp Primers Pools A&B( (= & )) ,000 TaqMan PreAmp Master Mix TaqMan PreAmp Master Mix ,000 11
12 mirnapcr Human TaqMan Array Human MicroRNA Cards TaqMan Array Human MicroRNA A+B Cards Set v ,000 A array ( ) + B Array ( ) 4 TaqMan Array Human MicroRNA A Card v ,000 TaqMan Array Human MicroRNA B Card v ,000 TaqMan Array Human MicroRNA A+B Cards Set v3.0 A array ( ) + B Array ( ) ,000 TaqMan Array Human MicroRNA B Card v ,000 Rodent TaqMan Array Rodent MicroRNA Cards TaqMan Array Rodent MicroRNA A+B Cards Set v ,000 A array ( ) + B Array ( ) 4 TaqMan Array Rodent MicroRNA A Card v ,000 TaqMan Array Rodent MicroRNA B Card v ,000 TaqMan Array Rodent MicroRNA A+B Cards Set v3.0 A array ( ) + B Array ( ) ,000 TaqMan Array Rodent MicroRNA B Card v ,000 TaqMan MicroRNA Panels /Plate /Plate TaqMan OpenArray Human MicroRNA Panel QuantStudio TM 12K Flex * 99,000 TaqMan OpenArray Rodent MicroRNA Panel QuantStudio TM 12K Flex * 99,000 TaqMan OpenArray Real-Time Master Mix, 1.5mL 1.5 ml ,000 TaqMan OpenArray Real-Time Master Mix, 5mL 5 ml ,000 QuantStudio 12K Flex OpenArray Accessories Kit ,500 * QuantStudio 12K Flex PCR PCR QuantStudio 12K Flex PCR Applied Biosystems Viia TM 7 PCR 12
13 mirna mirna mirna mirna TaqMan PCR mirna TaqMan MicroRNA Assays mirna ~22 ntmirna mirna mirna 3 RT/miRNA RT TaqMan PCR C. elegans Sanger TaqMan MicroRNA Assays mirbase C. elegansmirbase mirna mirna TaqMan Assays mirbase mirna TaqMan MicroRNA Assays mirna 3' cdna TaqMan PCR mirna TaqMan (RT) MicroRNA PCR 4 5 Assay microrna microrna Stem-loop primer 30 min 65 min 25 min 90 min mirvana mirna Isolation Kit TaqMan mirna ABC Purification Kit RecoverAll Total Nucleic Acid Isolation Kit for FFPE TaqMan MicroRNA Cells-to-CT Kit TaqMan MicroRNA Reverse Transcription Kit TaqMan MicroRNA Assays TaqMan Universal Master Mix II TaqMan MicroRNA Assays Applied Biosystems PCR Instrument software (included) RQ Manager enterprise software (optional) RealTime StatMiner Software (optional) 13
14 TaqMan MicroRNA Assays 2TaqMan MicroRNA Assays TaqMan Sanger mirbasemirna Sanger mirbase TaqMan MicroRNA Assays Custom TaqMan Small RNA Assays mirnataqman mirna Assay 200 Custom TaqMan Small RNA Assay RNA mirna pirna piwiinteracting RNA snrna snorna small RNA C. elegans RNA Endogenous controls for real-time quantitation of mirna using TaqMan MicroRNA Assays TaqMan mirna Target Sequence Stem-loop RT primer mirna ng RNA 7 log 1 mirna 7 Forward Primer Reverse Transcription Real-Time PCR 2 PCR Q TaqMan probe F Reverse primer 6. TaqMan MicroRNA Assay 2 mirna PCR 14
15 Mature mirnas Precursors mirna Assays Perfectly Mismatched C t C t (mismatch matched C t vs. match) C t C t (Precursor vs. mature) let-7a Looped Linear mirna mirna Stem-loop RT primer Linear RT primer 7. TaqMan MicroRNA AssaysmiRNA let-7arttaqman MicroRNA AssayRT/PCR/TaqMan let-7a let-7e let-7a 2 mirna1.5 x 10 8 mirna let-7a TaqMan MicroRNA Assay RT RT PCR RTmiRNA mirna mirna Δ Rn 0.1 C T 25 70M copies 7 copies Slope = 3.36 R 2 = , Cycle Panel A. Amplification Plot Quantity Panel B. Standard Curve 8. TaqMan MicroRNA Assays 7 log 1 mirna mirna TaqMan MicroRNA Assays lin-4 mirnalin-4 TaqMan MicroRNAAlin-4 mirna 7 PCR RNA1.3 x 10-3 fm71.3 x 10 4 fm7 x 10 7 B lin-4 mirna 15
16 Ordering information microrna mirvana mirna Isolation Kit TaqMan mirna ABC Purification Kit microrna RT TaqMan MicroRNA RT Kit TaqMan MicroRNA RT KitTaqManR MicroRNA Assays TaqMan MicroRNA AssaysRT TaqMan MicroRNA RT Kit ,000 TaqMan MicroRNA RT Kit 1, ,000 mirnapcr TaqMan MicroRNA Assays TaqMan MicroRNA Assays PCR microrna mirnamirna TaqMan MicroRNA Assays, Inventoried ,800 mirnart primer 5X reaction mix(50 / 15 µl) mirnataqman Assay 20X reaction mix (150 / 20 µl) Arabidopsis thaliana Caenorhabditis elegans Drosophila melanogaster TaqMan MicroRNA Assays, Made-to-Order, Extra Small mirnart primer 5X reaction mix(25 / 15 µl) mirnataqman Assay 20X reaction mix (75 / 20 µl) ,000 TaqMan MicroRNA Assays, Made-to-Order, Small mirnart primer 5X reaction mix(50 / 15 µl) mirnataqman Assay 20X reaction mix (150 / 20 µl) ,000 TaqMan MicroRNA Assays, Made-to-Order, Medium ,000 TaqMan MicroRNA Assays, Made-to-Order, Large 2,900 2, ,000 Custom TaqMan Small RNA Assays * Custom TaqMan Small RNA Assays, X ,000 Custom TaqMan Small RNA Assays, ,000 Custom TaqMan Small RNA Assays, 750 2, ,000 Custom TaqMan Small RNA Assays, 2,900 8, ,000 *20µL 16
17 TaqMan Universal Master Mix II TaqMan Universal Master Mix IITaqManTaqMan MicroRNA Assays PCR TaqMan Universal Master Mix II, with UNG * TaqMan Universal Master Mix II, with UNG 1mL ,000 TaqMan Universal Master Mix II, with UNG 5mL ,000 TaqMan Universal Master Mix II, with UNG 2 x 5mL ,000 TaqMan Universal Master Mix II, with UNG 5 x 5mL 1, ,000 TaqMan Universal Master Mix II, with UNG 10 x 5mL 2, ,000 TaqMan Universal Master Mix II, with UNG 50mL 2, ,000 TaqMan Universal Master Mix II, no UNG * TaqMan Universal Master Mix II, no UNG 1mL ,000 TaqMan Universal Master Mix II, no UNG 5mL ,000 TaqMan Universal Master Mix II, no UNG 2 x 5mL ,000 TaqMan Universal Master Mix II, no UNG 5 x 5mL 1, ,000 TaqMan Universal Master Mix II, no UNG 10 x 5mL 2, ,000 TaqMan Universal Master Mix II, no UNG 50mL 2, ,000 *50µL PCR QuantStudio 12K Flex PCR Applied Biosystems ViiA 7 PCR Applied Biosystems 7500 Fast PCR Applied Biosystems 7500 PCR StepOnePlus TM /StepOne TM PCR 17
18 microrna mirna mirna mirna mimic gain-of-function loss-of-function mirna inhibitor down-regulation mirnaup-regulationdown-regulation mirna Ambion 1 mirnaambion microrna mimic inhibitormirna Ambion mirvana mirna mimic mirvana mirna inhibitormirna gain-of-function loss-offunctionsirna gain-of-function loss-of-functionmirna mirna PCR mirvana mirna mimicmirvana mirna inhibitor mirna mrna mirnamirna / mrna mirna mirna mimic inhibitor mrna 3' 5' mirvana mirna mimic 3' 5' mirvana mirna Inhibitor mirvana mirna mimics mirvana mirna inhibitors Lipofectamine RNAiMAX Transfection Agent Neon Transfection System TaqMan Gene Expression Cells-to-CT Kit TaqMan Gene Expression Assays Applied Biosystems PCR NuPAGE iblot MagicMark XP mirna In vitro In vivo mirna mirvana mirna mimics & inhibitors Lipofectamine RNAiMAX mirna mirvana mirna mimics & inhibitors, in vivo ready Invivofectamine
19 microrna mirvana mirna mimics & inhibitors: mirbase in vitro in vitroin vivo mrna upregulation % mir 122 mirna122 = mir122 mirna inhibitor NT =Not transfected Neg1 = Negative Control #1 NT AldoA NEG1 mir 122 NT NEG1 mir NT NEG1 mir NT Hfe2 Slc35a4 Lass6 NEG1 mirvana mirna mimics mirvana mirna mimics RNA sirna mirna mirna RISC RNAinduced silencing complex 8 sirna mirna Dose response mirvana mirna Inhibitors mirvana mirna Inhibitor mirna mirna inhibitor mirvana mirna Inhibitor 4 mirna in vivo in vivo Ready mirnain vivo TaqMan mirvana mrna inhibitor TaqMan 10 mimic inhibitor in vivo 1 mimic inhibitor 10. mirvana mirna inhibitor in vivomirna mir122negative Control #1 mirvana mirna inhibitor Invivofectamine 2.0Balb-C 37 mg/kg 24 mir122 4TaqMan MicroRNA AssayNegative ControlmiR1224 mrna Fold change mirna mimic Neg reporter 1 mirna mimic Neg mirna mimic 11. mirvana mirna mimic mirna maturestar maturestar 6miRNAmirVana mirna mimic mature Negative Control 510 star Negative Control mirna Neg mirna mimic mirnamirna mirvana mirna mimicinhibitor nmol 50 nm 50mirVana mirna mimicinhibitor Positive Control Negative Control Neg mirna mimic Neg mirna mimic reporter 2 reporter 3 reporter 4 reporter 5 reporter 6 FW RV Neg mirvana mirna mimics 3' 5' 3' 5' 5'Cap AAAAAAAAAAAAA3' 3' 5' No translation mirna mimic Sequence-specific loading into RISC-like complex mirvana mirna inhibitors mrna 3' 5' 3' 5' 5'Cap AAAAAAAAAAAAA3' Translation mirna inhibitor Complementary binding of specific mirnas no RISC-like complex association 3' 5' 9. mirvana mirna mimics mirvana mirna inhibitors 19
20 Ordering information microrna microrna mirvana mirna mimic mirvana mirna mimic 5 nmol ,000 mirvana mirna mimic 20 nmol ,000 mirvana mirna mimic, in vivo Ready 250 nmol ,000 Customer-defined mirvana mirna Mimic 5 nmol ,000 Customer-defined mirvana mirna Mimic 20 nmol ,000 Customer-defined mirvana mirna Mimic, in vivo Ready 250 nmol ,000 mirvana mirna Mimic Negative Control #1 5 nmol ,000 mirvana mirna Mimic Negative Control #1 20 nmol ,000 mirvana mirna Mimic Negative Control #1, in vivo Ready 50 nmol ,000 mirvana mirna Mimic Negative Control #1, in vivo Ready 250 nmol ,000 mirvana mirna Mimic mir-1 Positive Control 5 nmol ,000 mirvana mirna Mimic mir-1 Positive Control 20 nmol ,000 mirvana mirna Mimic mir-1 Positive Control, in vivo Ready 50 nmol ,000 mirvana mirna Mimic mir-1 Positive Control, in vivo Ready 250 nmol ,000 mirvana mirna mimics mirbase v 19* 0.25 nmol each mirvana mirna mimics mirbase v 19* 1 nmol each mirvana mirna inhibitor * mirvana mirna inhibitor 5 nmol ,000 mirvana mirna inhibitor 20 nmol ,000 mirvana mirna inhibitor, in vivo ready 250 nmol ,000 Customer-defined mirvana mirna Inhibitor 5 nmol ,000 Customer-defined mirvana mirna Inhibitor 20 nmol ,000 Customer-defined mirvana mirna Inhibitor, in vivo ready 250 nmol ,000 mirvana mirna Inhibitor Negative Control #1 5 nmol ,000 mirvana mirna Inhibitor Negative Control #1 20 nmol ,000 mirvana mirna Inhibitor Negative Control #1, in vivo Ready 50 nmol ,000 mirvana mirna Inhibitor Negative Control #1, in vivo Ready 250 nmol ,000 mirvana mirna Inhibitor let-7 Positive Control 5 nmol ,000 mirvana mirna Inhibitor let-7 Positive Control 20 nmol ,000 mirvana mirna inhibitor mirbase v 19* 0.25 nmol each mirvana mirna inhibitor mirbase v 19* 1 nmol each *
21 mirna & Lipofectamine RNAiMAX reagent sirna RNAi mirna mimicmirna inhibitor Neon Transfection System Neon sirna/mirna sirna Invivofectamine 2.0 sirna mirvana mirna mimics and inhibitors, in vivo readyin vivo mirna mirna & Lipofectamine RNAiMAX 12. Neon Transfection Neon Lipofectamine RNAiMAX 0.1 ml ,800 Lipofectamine RNAiMAX 0.3 ml ,000 Lipofectamine RNAiMAX 0.75 ml ,000 Lipofectamine RNAiMAX 1.5 ml ,000 Lipofectamine RNAiMAX 15 ml ,000 Invivofectamine 2.0 In vivo Invivofectamine 2.0 Reagent Starter Kit 1mL ,000 Invivofectamine 2.0 Reagent 5mL ,000 Starter kitsirna Ambion In Vivo FVll sirna50nmol Neon Transfection System Neon Transfection System* 1 1 MPK ,000 Neon Transfection System Starter Pack* 2 1 MPK5000S 1,050,000 1 Neon Transfection System(MPK5000) Neon Neon Pipette MPP100Neon Pipette Station MPS100 2 Starter Pack MPK5000S Neon Transfection System(MPK5000) Transfection Kit(MPK10096 MPK10961 Neon Transfection System Neon Transfection System Kit (100 L) 1 Neon 96 MPK ,000 1 Neon 25 MPK ,000 Neon Transfection System Kit (10 L) 1 Neon 96 MPK ,000 1 Neon 25 MPK ,000 Neon Transfection Tubes 1 (100 ) MPT100 49,000 Neon Pipette MPP100 75,000 Neon Pipette Station MPS ,000 21
22 mirna mirnagain-of-function loss-of-function mrna PCR TaqMan PCR SYBR PCR micrornamrna RNA microrna PCR Reagents and Consumables Catalog & 22
23 microrna mirnasmall RNA small RNA <200 nt mirna TaqMan mirna ABC Purification Kit TaqMan mirna ABC anti-mirna Bead Capture Purification KitTaqMan mirna Assay TaqMan Array Cardanti-miRNA FFPE mirna 10 L 50 LmiRNA PCR TRIzol 75 RNA TaqMan mirna ABC Purification KitmiRNA 2 1Human Panel A Human Panel B TaqMan Array Human MicroRNA A Card B Card 754 mirnamirna mirna mirvana mirna Isolation Kit Lysis Solution RNase RNA RNA RNA RNA mirna mirna small RNA small RNA RNAsmall RNA<200 nt RecoverAll Total Nucleic Acid Isolation Kit FFPE RecoverAll Kit 451.5FFPE RNAFFPE RNA DNA RNA PCR TaqMan MicroRNA Cells-to-CT Kit 10PCR RNA PBS 8DNase Stop Solution TaqMan MicroRNA Cells-to-CT Kit 10 TaqMan MicroRNA TaqMan Universal PCR Master Mix II TaqMan mirna 23
24 microrna mirvana mirna Isolation Kit mirvana PARIS Kit MagMAX -96 for Microarrays Total RNA Isolation Kit TaqMan MicroRNA Cells-to-C T Kit TaqMan mirna ABC Purification Kit RecoverAll Total Nucleic Acid Isolation Kit for FFPE Tissues TRI Reagent Cells-to-C T DNase RNA PCR Anti-miRNA mirna mg mg 5x mg L mg FFPE5 10 m 50 L FFPE 20 m RNase small RNA mirna 30 RNA small RNA small- RNA mrna mirna sirna small RNA 10 PCR TaqMan Micro RNA Array Card Panel TRIzol 75 RTqPCR TaqMan Human MicroRNA FFPE DNA mirna Proteinase K PCR >50% PCR PCR 24
25 Ordering information microrna micrornatotal RNA mirvana mirna Isolation Kit mirvana mirna Isolation Kit 40 AM ,300 mirvana mirna Isolation Kit without Phenol 40 AM ,000 mirna mirvana PARIS Kit mirvana PARIS Kit 40 AM1556M 57,800 MagMax MagMAX Total Nucleic Acid Isolation Kit 100 AM ,000 MagMAX -96 Blood RNA Isolation Kit 96 AM ,800 MagMAX -96 Total RNA Isolation Kit 96 AM ,500 MagMAX Viral RNA Isolation Kit 50 AM ,000 MagMAX -96 Viral RNA Isolation Kit 96 AM ,900 RNA Single Place Magnetic Stand 1 AM ,400 Magnetic Stand-96 1 AM , well Magnetic-Ring Stand 1 AM ,000 TaqMan MicroRNA Cells-to-C T Kit RNA microrna mirna * TaqMan MicroRNA Cells-to-C T Kit ,000 * microrna TaqMan mirna ABC Anti-miRNA Bead Capture Purification Ki TaqMan mirna ABC Purification Kit-Human Panel A ,000 TaqMan mirna ABC Purification Kit-Human Panel B ,000 25
26 Total Exosome Isolation Total Exosome Isolation RNA RNA RNAPCR Total Exosome Isolation 20 2 Total Exosome RNA & Protein Isolation Kit 30-60RNA RNA Exosome Immunoprecipitation Protein A/G mirna Total Exosome Isolation Total Exosome Isolation HeLa Total Exosome Isolation NanosightLM nm nm Average Ct Values hsa-let7e hsa-mir26a HeLa Total Exosome Isolation Total Exosome RNA and Protein Isolation Kit RNATaqMan RT- PCR mirna Ordering information Total Exosome Isolation 50ml ,000 Total Exosome Isolation 6ml ,000 Total Exosome RNA & Protein Isolation Kit ,000 Exosome Immunoprecipitation Protein A * 1ml 10610D 22,000 Exosome Immunoprecipitation Protein G * 1ml 10612D 22,000 * References 1. Bartel DP (2004) MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116(2): Soifer HS, Rossi JJ, Saetrom P (2007) MicroRNAs in disease and potential therapeutic applications. Mol Ther 15(12): Griffiths-Jones S, Saini HK, van Dongen S, Enright AJ (2008) mirbase: tools for microrna genomics. Nucleic Acids Res 36: D154 D
27 TaqMan Assays ID TaqMan MicroRNA Assays mirvana mirna mimics mirvana mirna inhibitors Applied Biosystems ID JPOrderAdminCS appliedbiosystems.com
28 公式 Facebook ページ & Twitter もチェック 記載の価格は2012年現在の価格です 消費税は含まれておりません 価格は予告なしに変更する場合がありますので予めご了承ください 研究用にのみ使用できます 診断目的およびその手続き上での使用は出来ません 記載の社名および製品名は 弊社または各社の商標または登録商標です The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan and AmpliTaq Gold are registered trademarks of Roche Molecular Systems, Inc., used under permission and license. RealTime StatMiner is a registered trademark of Integromics, S.L. 2012, Life Technologies Japan Ltd. All rights reserved. Printed in Japan. GNL130-A1212OB 販売条件はこちらをご覧ください 取扱店 ライフテクノロジーズジャパン株式会社 本社 東京都港区芝浦 TEL FAX 大阪 大阪府吹田市広芝町 TEL FAX MicroRNA_brochure_1205b.indd 28 12/12/05 16:36
MicroRNA RNA mirna RNA [1*] mirna mirna [2*] mirna RNA mirna mirnasmall RNA mirna mirna mirna 2 *Reference
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