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1 NEXT / October / No.15 NEXT Interview P. 04 Ion Proton P. 05 QuantStudio 12K Flex PCR P. 06 SYBR Select Master Mix P. 07 Total Exosome Isolation P. 09 Essential 8 Interview P. 10 GeneArt Seamless PLUS Cloning and Assembly Kit P. 12 FLoid P. 13 phrodo green bio particle P. 14 Tali P. 15 Attune Auto Sampler Invitrogen Applied Biosystems Gibco Molecular Probes Novex TaqMan Ambion Ion Torrent

2 NEXT Interview: Mitinori Saitou, M.D., Ph.D. ips ips ips ES BMP4 ips ES ips

3 ips 10 ips ES ips ES Reconstitution of the mouse germ cell specification pathway in culture by pluripotent stem cells. Hayashi K. et.al Cell, 146, Germ cell specification in mice: Signalling, transcription regulation and epigenetic consequences. Saitou, M., and Yamaji, M. Reproduction, 139, ES lifescience.mext.go.jp/files/pdf/40_126.pdf i P S Activin bfgfgibco KSR KnockOut SR FBS invitrogen PCR Applied Biosystems Wellcome Trust Travelling Research Fellow Senior Research Associate JST ERATO

4 04 Ion Torrent 1000 Ion Proton Ion Torrent Ion Proton Ion Proton I chip 2 10Gb 4 10 AmpliSeq Ion AmpliSeq Comprehensive Cancer Panel Ion AmpliSeq Inherited Disease Panel Gb 1 10 NEW! Ion Proton * x 35 bp 1 x 100 bp 1 x 200 bp 1.5 Gb 5 Gb 10 Gb Ion Proton I chip 50 M 50 M 50 M Gb 30 Gb 60 Gb Ion Proton II chip* 200 M 200 M 200 M Ion Proton PROTON ,600,000 Ion Proton I chip ,000 Ion Proton I chip ,000 Ion Proton I Template OT2 Kit ,000 Ion Proton I Sequencing Kit ,000 Ion Proton Controls Kit ,000 User's Voice User's Voice NEXTNEXT URL NEXT lifeinfo.jp@lifetech.com

5 TaqMan / Applied Biosystems 05 Ion PGM & Proton!! QuantStudio 12K Flex PCR castpcr PCR 1! castpcr!! PCRPCR 1 12, / 384 / 3,072 TaqMan Array Card Applied Biosystems PCR 2!! castpcr Competitive Allele-Specific TaqMan PCR MGB 0.1% Ion AmpliSeq Cancer Panel TaqMan Mutation Detection Assays castpcr TaqMan Mutation Detection Assay castpcr PCR PCR2 TaqMan SNP Genotyping Assays 0.01% DNA 1 PCR NEXT No.14 P.10 i am TaqMan PCR gdna cdna RNA well Copy Number Variation TaqMan Mutation Detection Assays 150 l ,000 TaqMan Mutation Detection Reference Assays 150 l ,000 QuantStudio 12K Flex PCR 1

6 06 Applied Biosystems PCR SYBR Select Master Mix PCR MasterMix DNA SYBR GreenER PCR72 PCR NEW! SYBR SelectMasterMix Applied Biosystems ViiA 7PCR cdna cdnapgk1 PCR 100ng cdna 1ng 2ng cdna SYBR PCR 8A2M ACACA AOF1 APOA1 ARL1-actin BMP2 CCNB1 20 SYBR Select Master Mix, Mini Pack 1 ml ,700 SYBR Select Master Mix, 1-Pack 1 x 5 ml ,800 SYBR Select Master Mix, 2-Pack 2 x 5 ml 1, ,800 SYBR Select Master Mix, 5-Pack 5 x 5 ml ,000 SYBR Select Master Mix, 10-Pack 10 x 5 ml 5, ,000 SYBR Select Master Mix, Bulk Pack 1 x 50 ml 5, ,000

7 NEW! Invitrogen 07! Total Exosome Isolation mrna mirna Total Exosome Isolation RNA RNA RNART-PCR Total Exosome Isolation 20 2 Total Exosome RNA & Protein Isolation Kit 30-60RNA RNA Exosome Immunoprecipitation Protein A/G Total Exosome Isolation mirna Total Exosome Isolation Total Exosome Isolation HeLaTotal Exosome Isolation NanosightLM10 300nm nm HeLaTotal Exosome Isolation Total Exosome RNA and Protein Isolation Kit RNA TaqMan RT-PCR mirna Total Exosome Isolation 50ml ,000 Total Exosome Isolation 6ml ,000 Total Exosome RNA & Protein Isolation Kit ,000 Exosome Immunoprecipitation Protein A * 1ml 10610D 22,000 Exosome Immunoprecipitation Protein G * 1ml 10612D 22,000 *

8 08 Gibco 50 Gibco 50 10NEXT 2012P16 Gibco Hero of the Month 9HERO URL Gibco Hero of the Month WPI-iCeMS ES ES 2007 WPI-iCeMS 50 Gibco Gibco e m b r yonal carcinoma cell line Gibco Gibco Gibco URL

9 Gibco 09 Essential 8 Guokai Chen Cellular Dynamics International N VTN-N Essential8 Essential 8 500mL 10mL ESSENTIAL 8 500ml A14666SA 19,800 VTN-N 1ml A14701SA 5,000 Gibco Essential 8 Emile Nuwaysir, Ph.D. Chief Operating Officer and Vice President / Cellular Dynamics International Essential BSA Essential 8 BSA BSA BSA BSA BSA BSA 1 g/ml 1 g BSA BSA 8 BSA GMP GMP GMP GMP GMP GMP Essential 8 Cellular Dynamic International CDI Essential 8 Essential MEF Essential 8

10 10 Invitrogen GeneArt GeneArt Seamless PLUS Cloning and Assembly Kit 4PCR HTP 40kb 90% pre-cloning conjugative vector NEW! DNA 30 One Shot Chemically Competent TOP10 E. coli PCR GeneArt Seamless Cloning and Assembly Enzyme Mix NEW NEW GeneArt Seamless Cloning and Assembly Enzyme Mix GeneArt Seamless Cloning and Assembly Kit >90% 90% - single 5kb DNA >90% GeneArt Seamless PLUS Cloning and Assembly Kit 70% - 4 Fragments, 1kb each 3 40% - 4 DNA Fragments, 2kb each Up to 4 Up to 4 Up to 4 13 kb 13 kb 40 kb 1 E. coli E. coli E. coli 1 1,800 2,400 2,750 Linear cloning vector Control inserts All-in-one, RT-stable enzyme/buffer mix Linear cloning vector Control inserts Competent cells Enzyme mix Media OneShot TOP10 Chemically Competent E. coli Linear cloning vectors Control inserts All-in-one, RT-stable enzyme/ buffer mix Competent cells Media OneShot DH10B T1 SA Chemically Competent E. coli Conjugative transfer vector GeneArt Seamless Cloning and Assembly Enzyme Mix 20 A ,000 GeneArt Seamless Cloning and Assembly Kit 20 A ,000 GeneArt Seamless PLUS Cloning and Assembly Kit 20 A ,000

11 Invitrogen 11 Technical Review Lipofectamine Lipofectamine 20 48,000 R&D Xavier de Mollerat du Jeu R D Xavier Lipofectamine * * TOP5 1 Lipofectamine LTX PLUS sirna/mirnalipofectamine RNAiMAXNeon DNA sirna/mirna Lipofectamine 5 DNA sirna/mirna DAY 0 DAY 1 DAY % Opti-MEM 4 Lipo fectamine LTX O p t i - M E M D N A Plus Lipofectamine LTX DNA 1:1 DNA- PureLink Plasmid DNA Miniprep Kit DNA 0.5 1µg/µl DNA Silencer Select sirnamirvana mirna mimicsinhibitorsirna/ mirna Lipofectamine life technolo g i e s. co m / lipofectamine Lipofectamine 2000 Lipofectamine LTX Lipofectamine RNAiMAX Neon Transfection System *2 sirna/mirna DNA *1 RNA *1 0.3ml 0.1ml 0.1ml 23,000 8,500 8, , A MPK5000 *1 DNA sirnalipofectamine 2000*2

12 12 Molecular Probes FLoid m 400%8025 m 100 m 100 m 100 m WGA HeLa GFP BPAE FLoid Cell Imaging Station ,980,000

13 NEW! Molecular Probes 13 ph Bio Particle phrodo green bio particle phphrodo Green ph ph phrodo FITC! phrodo Green Dextran, 10,000 MW, for Endocytosis 0.5 mg P ,000 phrodo Green E. coli BioParticles Conjugate for Phagocytosis 5 x 2 mg P ,000 phrodo Green S. aureus Bioparticles Conjugate for Phagocytosis 5 x 2 mg P ,000 phrodo Green Zymosan Bioparticles Conjugate for Phagocytosis 5 x 1 mg P ,000 phrodo Red Zymosan Bioparticles Conjugate for Phagocytosis 5 x 1 mg P ,000 phrodo Green Maleimide 1 mg P ,000 phrodo Red Maleimide 1 mg P ,000 NEW! CD117 MOUSE 104D2 PE-Cy 7 A ,000 CD137 MOUSE 4B4-1 PE A ,000 CD278 MOUSE ISA-3 APC A ,000 CD278 MOUSE ISA-3 PE-Cy 7 A ,000 CD278 MOUSE ISA-3 FITC A ,000 CD278 MOUSE ISA-3 PE A ,000 CD45 MOUSE HI30 PerCP-Cy 5.5 A ,000 SSEA-4 MOUSE MC PE A ,000 SSEA-4 MOUSE MC Alexa Fluor 488 A ,000 CD11b RAT M1/70 PerCP-Cy 5.5 A ,000 CD11c HAMSTER N418 PerCP-Cy 5.5 A ,000 CD127 RAT A7R34 PE A ,000 CD127 RAT A7R34 PerCP-Cy 5.5 A ,000 CD127 RAT A7R34 Alexa Fluor 647 A ,000 CD127 RAT A7R34 Alexa Fluor 488 A ,000 CD144 RAT BV13 APC A ,000 CD150 RAT 9D1 PE A ,000 CD150 RAT 9D1 FITC A ,000 CD150 RAT 9D1 APC A ,000 CD16/32 RAT 93 PerCP-Cy 5.5 A ,000 CD1d RAT 1B1 Alexa Fluor 488 A ,000 CD1d RAT 1B1 PE A ,000 CD24 RAT M1/69 PerCP-Cy 5.5 A ,000 CD24 RAT M1/69 PE-Cy 7 A ,000 CD274 RAT MIH5 PE A ,000 CD278 HAMSTER C398.4A FITC A ,000 CD279 RAT RMP1-30 APC A ,000 CD279 RAT RMP1-30 FITC A ,000 CD279 RAT RMP1-30 PE A ,000 CD335 RAT 29A1.4 PE A ,000 CD335 RAT 29A1.4 FITC A ,000 CD3e HAMSTER 145-2C11 PE-Cy 7 A ,000 CD3e HAMSTER 145-2C11 PerCP-Cy 5.5 A ,000 CD4 RAT RM4-5 PerCP-Cy 5.5 A ,000 CD44 RAT IM7 PerCP-Cy 5.5 A ,000 CD45 RAT 30-F11 PerCP-Cy 5.5 A ,000 CD45.1 MOUSE A20 PerCP-Cy 5.5 A ,000 CD45.2 MOUSE 104 PerCP-Cy 5.5 A ,000 CD45R RAT RA3-6B2 PerCP-Cy 5.5 A ,000 CD48 HAMSTER HM48-1 PE A ,000 CD48 HAMSTER HM48-1 PE-Cy 7 A ,000 CD62L RAT MEL-14 PerCP-Cy 5.5 A ,000 CD8a RAT PerCP-Cy 5.5 A ,000 CD90.1 MOUSE HIS51 PerCP-Cy 5.5 A ,000 CD90.2 RAT PE-Cy 7 A ,000 F4/80 RAT BM8 PerCP-Cy 5.5 A ,000 IL-17A RAT 17B7 PE-Cy 7 A ,000 IL-17A RAT 17B7 Alexa Fluor 647 A ,000 IL-17A RAT 17B7 PerCP-Cy 5.5 A ,000 IL-17A RAT 17B7 PE A ,000 KLRG1 HAMSTER 2F1 APC A ,000 Ly-6A/E RAT D7 PerCP-Cy 5.5 A ,000 Ly-6A/E RAT D7 PE-Cy 7 A ,000 Ly-6C/G RAT RB6-8C5 PerCP-Cy 5.5 A ,000 MHC RAT M5/ FITC A ,000 MHC RAT M5/ PE A ,000 MHC RAT M5/ PE-Cy 7 A ,000 MHC RAT M5/ APC A ,000 NK1.1 MOUSE PK136 PerCP-Cy 5.5 A ,000

14 14 Invitrogen Tali 2 RFP Fluorescence SYTOX Blue Fluorescence RFP GFP Tali 50 1 T ,480,000 Tali Cellular Analysis Slides T ,000 Tali Cellular Analysis Slides T ,000 User's Voice Tali E L I S A ELISA Tali 19 Tali Tali Voice of Customer URL: nextforum

15 Invitrogen / Applied Biosystems 15 1 attp Jump-In Gateway Expression System Japan Uniformity Manufacture: ISO9001 Position Inexpensive Non Isolation Jump-In ,000 Attune Auto Sampler Attune Attune Acoustic Focusing Cytometer A B A TUBE B Plate OK 96/ % CD8 R-PE Fluorescence CD8 R-PE Fluorescence CD4 FITC Fluorescence CD4 FITC Fluorescence Tube statistics Plate statistics CD8 + /CD4 24.5% CD8 + /CD4 24.6% CD8 + /CD % CD8 + /CD % CD8 /CD4 25.6% CD8 /CD4 24.7% CD8 /CD % CD8 /CD % Attune Acoustic Focusing Cytometer Auto Sampler Upgrade Kit

16 INFORMATION NEXT No.15 / 2012 / October NEXT 2012] NEXT Ion Torrent 10 3 A5 2Gibco NEXT NEXT 10 No.15? NEXT10 2,000 URL PDF URL The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is registered trademark of Roche Molecular Systems, Inc. Used under Permission and license. 2012, Life Technologies Japan Ltd. All rights reserved. Printed in Japan TEL FAX LFT034-A1209OB

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