九州大学学術情報リポジトリ Kyushu University Institutional Repository リアルタイム PCR : ソノゲンリトトクチョウ 小島, 夫美子九州大学医学部保健学科検査技術科学専攻 大阪大学大学院医学系研究科保健学専攻 岩谷, 良則大阪大学大学院医学系研究科保健学
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1 九州大学学術情報リポジトリ Kyushu University Institutional Repository リアルタイム PCR : ソノゲンリトトクチョウ 小島, 夫美子九州大学医学部保健学科検査技術科学専攻 大阪大学大学院医学系研究科保健学専攻 岩谷, 良則大阪大学大学院医学系研究科保健学専攻 藤本, 秀士九州大学医学部保健学科検査技術科学専攻 出版情報 : 九州大学医学部保健学科紀要. 2, pp , School of Health Sciences, Faculty of Medicine, Kyushu University バージョン : 権利関係 :
2 Memoirs Kyushu U. Sch. Health Sci., 2003 vol Fumiko Kojima Yoshinori Iwatani Shuji Fujimoto key word: real-time PCR, quantitative PCR, melting curve analysis Polymerase Chain Reaction PCR PCR DNA PCR PCR real-timepcr PCR DNA PCRDNA PCR PCR DNA DNA PCR
3 96 PCR PCR PCR PCR PCR ABI PRISM ABI Light Cycler Roch Smart Cycler Cepheid icycler iq BioRad PCRDNA DNA PCR 9 DNA DNA DNA DNA DNA DNA DNA DNA DNA DNA DNA DNA n n-1 PCR PCR 12 DNA DNA
4 97 DNA 2DNA 1 1DNA 1DNA 2 1DNA 3 DNA 2DNA cdna 2DNA DNA SYBR Green 13 SYBR GreenDNA PCR DNA SYBR Green DNA DNA PCRDNA TaqMan PCR 53 TaqMan PCR DNA DNA TaqMan
5 98 PCR Molecular Beacon DNA DNA 14 1 Forward primer SYBR Green I DNA DNA DNA TaqMan Forward primer R TaqMan Q DNA R DNA DNA Q DNA Molecular Beacon Molecular Beacon R Q R Q DNA
6 99 DNA 14 PCR 16S rrna DNA PCR A A D DNAA:N 10 8 B:N 10 7 C:N 10 6 D:N 10 5 DNA B DNAlogCt Ct DNA
7 100 PCR PCR SYBR Green Smart Cycler Cepheid PCR PCR PCR PCR PCR DNA PCR A PCR DNA SYBR Green I Tm B -df/dt Tm
8 101 PCR DNA PCR threshold cycle : Ct Ct DNA PCRCt DNA TaqMan Molecular Beacon SYBR Green DNA DNA PCRDNA Melting Temperature:Tm PCR 0.2 / Melting Curve DNA DNA DNA SYBR Green I DNA TmPCR Tm GC TmPCR 17 -df/dt Tm PCR RT Reverse Transcription PCR total RNA mrnacdna cdna PCR RNA RNA 18 Mullis KB and Faloona F: Specific synthesis of DNA in vitro via a polymerase-catalysed chain reaction. Methods. Enzymol.155: , Lomeri H, Tyagi S, Prichard CG, Lizardi PM, and Kramer FR: Quantitative assays based on the use of repricatable hybridization probes. Clin. Chem. 35: , Kidd IM, Clark DA and Emercy VC: A non-radioisotopic quantitative competitive polymerase chain reaction method: application in measurement of human herpesvirus 7 load. J. Virol. Methods. 87: , Guatelli JC, Gingeras TR and Richman DD: Nucleic acid amplification in vitro: detection of sequences with low copy numbers and application to diagnosis of human immunodeficiency virus type 1 infection. Clinic. Microbiol. Rev. 2: , Holland PM, Abramson RD, Watson R, and Gelfand DH: Detection of specific polymerase chain reaction product by utilizing the 5-3 exonuclease activity of Thermas aqaticus. Proc. Natl. Acad. Sci. USA 88: , Lee LG, Connell CR, and Block W: Allelic discrimination by nick-translation PCR with
9 102 PCR fluorogenic probes. Nucleic. Acids. Res. 21: , Livak KJ, Flood SJA, Marmaro J, Giusti W, and Deetz K: Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR methods Appl. 4: , Heid CA, Stevens J, Livak KJ and Williams PM: Real time quantitative PCR. Genome Res. 6: , Gibson UEM, Heid CA, and Williams PM: A novel method for real time quantitative RT-PCR. Genome Res. 6: , : , PCR , Wittwer CT, Ririe KM, Andrew RV, et al : The LightCycler TM : A Microvolume multisample fluorometer with rapid temperature control. Biotechniques 22: , Morisson TM, Weis JJ, and Wittwer CT: Quantification of low-copy transcripts by continuous SYBR green I monitoring during amplification. Biotechniques 24: , Tyagi S, and Kramer FR: Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14: , Tyagi S, Marras SAE, and Kramer FR: Multicolor molecular beacons for allele discrimination. Nat. Biotechnol. 16:49-53, Wetmer JG: DNA probes: application of the principles of nucleic acid hybridization. Crit. Rev. Biochem. Mol. Biol. 26: , Ririe KM, Rasmussen RP, and Wittwer CT: Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245: , Bustin SA: Quantification of mrna using realtime reverse transcription PCR RT-PCR : trends and problems. J. Mol. Endocrinol. 29:23-39, 2002.
Memoirs Dep. of Health Scis. Sch. of Med Kyushu Univ., 2006, vol.7, Kyoko WatanabeHarumi ShinkodaEtsuko Kitahara Key Words
九州大学学術情報リポジトリ Kyushu University Institutional Repository A Report of Nursing Students' Evaluations and Problemson Maternity Nursing Practice : Based on the Analysis of Reports after Baby Bath Practice
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九州大学学術情報リポジトリ Kyushu University Institutional Repository 看護師の勤務体制による睡眠実態についての調査 岩下, 智香九州大学医学部保健学科看護学専攻 出版情報 : 九州大学医学部保健学
九州大学学術情報リポジトリ Kyushu University Institutional Repository 看護師の勤務体制による睡眠実態についての調査 岩下, 智香九州大学医学部保健学科看護学専攻 https://doi.org/10.15017/4055 出版情報 : 九州大学医学部保健学科紀要. 8, pp.59-68, 2007-03-12. 九州大学医学部保健学科バージョン : 権利関係
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