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4 10:0010:10 Session- 10:1010:25 10:2510:40 10:4010:55 in-vivo 10:5511:10 11:1011: DNA Session- 11:2512:00 12:0012: JAMSTEC 3 12:1513:15 13:1513:30 13:3013:45 13:4514:00 3
5 Session- 14:0014:15 14:1514:30 14:3014:45 14:4515:00 Session- 15:0015: :1515: :3015:45 15:4516:20 16:2016:35 Session- 16:3516:55 16:5517:15 17:1517:50 17:50 URL 4
6 Function of iodine in marine phytoplankton ) µm KI 1 mm KI KIO 3 Emiliania huxleyi 2) 125 I-KI 125 I-KI SDS-PAGE Emiliania huxleyi Gephyrocapsa oceanica 1 mm KIO 3 20% KI Chaetoceros sociale KIO 3 Chlorella kessleri KI 3 Emiliania 1 mm KIO 3 1 mm KI 4 (530 µm) Emiliania 42D Emiliania 5
7 Screening and application of macroalgal genes involved in iodide accumulation Laminaria 60 ng/g 9 mg/g I 1 RNA DNA 2 : 1) Hou, X. et al. Sci Total Environ 204, (1997); 2) Zhu, Y. G. et al. Environ Int 29, (2003); 3) Riedel, C. et al. Trends Biochem Sci 26, (2001). 6
8 in vivo High accumulation of iodine in marine organisms and in vivo speciation of iodine in vivo In vivo kev Na-24 EPMA HPLCLC/MS/MSFT-IR X XAFS ring ppm I- 7
9 Isolation of iodine-accumulating bacteria from the environment 1) 125 I KI0.1 µm I KI0.1 µm 1.5 ml NaI C-12 U- 5 10,000 80% 16S rdna C-12 U-5 Flexibacter aggregans 8
10 DNA Evaluation of iodine effects using yeast DNA microarray ,2 DNA cdna DNA DNA Saccharomyces cerevisiae S288C(SUC2 mal mel gal2 CUP1)25 OD totalrna oligotex-dt30 (Takara) mrna mrna dt Cy5 Cy3 cdna cdna DNA (DNA )65 48 ScanArray(GSI Luminonics)GenePix (Inter Medical) GeneSpring (Silicon Genetics) OD6601 I 2 0mM0.5mM0.75mM1mM 2 2 1mM
11 Natural halogenated compounds in the atmosphere: Distribution and sources 30 ( ) ) Y. Yokouchi et al., A strong source of methyl chloride to the atmosphere from tropical coastal land, Nature, 403, (2000). 2) Y. Yokouchi et al., Atmospheric methyl iodide: High correlation with surface seawater temperature and its implications on the sea-to-air flux, J. Geophys. Res., 106, (2001). 3) Y. Yokouchi et al., Strong emission of methyl chloride from tropical plants, Nature, 416, (2002). 10
12 Relationship between distribution of halocarbon and species composition of phytoplankton in the equatorial Pacific 1 2 JAMSTEC ml 180ppm 4 - Agilent CH 2 Br 2 CHBr 2 ClCHBrCl 2 (3-5pM) 100m CHBr 3 CHBr 2 ClCHBrCl 2 ( pptv) C 2 Cl 4 CH 3 ClCH 3 Br 97 (15 ) Discosphaera sp. CH 3 Br 11
13 -Halogenated compunds from marine organisms; their formation mechanisms and physiological function- ( ) CHBr CH I 1) 2-) S- SAM 1 X - XCl, Br, I) X ( Enz. + H + X - + H 2 O 2 X + OH - (or Enz-X + OH - ) + H 2 O Sub.-H + X + OH - Sub.-X + H 2 O (scheme 1) SAM: CH 3 X) 2 CH 3 NH 2 X - + Ad-S-CH 2 CH 2 CHCOOH + Ad: adenosyl- S-adenosyl-L-methionine (SAM) CH 3 X + S-adenosyl-L-homocysteine(scheme 2) (SAH) 4 Corallinaceae ) SAM: Pseudomonas Co 2+ 12
14 (1996) Vol. 41, No.3, N. Itoh, Volatile haligenated compounds from marine algaem their formation mechanisms and geochemical aspects,recent Res. Devel. in Phytochem., (1997) 1, N. Ohsawa, Y. Ogata, N. Okada and N. Itoh, Physiological function of bromoperoxidase in the red marine alga, Corallina pilulifera: production of bromoform as an allelochemical and the simultaneous elimination of hydrogen peroxidase, Phytochemistry, (2001) 58, N. Ohsawa, M. Tsujita, S. Morikawa and N. Itoh, Purification and characterization of a monohalomethane-producing enzyme S-adenosyl-L-methionine: halide ion methyltransferase from a marine microalga, Pavlova pinguis, Biosci. Biotechnol. Biochem., (2001) 65, N. Itoh, T. Kawanami, J. Q. Liu, T. Dairi, M. Miyakoshi, C. Nitta and Y. Kimoto, Cloning and biochemical characterization of Co 2+ -activated bromoperoxidase-esterase (perhydrolase) from Pseudomonas putida IF-3 strain, Biochim. Biophys. Acta, (2001) 1545, T. Kawanami, M. Miyakoshi, T. Dairi and N. Itoh, Reaction mechanism of the Co 2+ -activated bromoperoxidase-esterase from Pseudomonas putida IF-3, Arch. Biochem. Biophys., (2002) 398,
15 Widespread occurrence of iodine-volatilizing bacteria in the environment : 129 I : % GC-ECDGC-MS CH 3 I 16S rdna Rhizobium sp. MRCD19 CH 3 I S- SAM CH 3 I 10 CH 3 I 2.5 pg/day/10 10 cells CH 3 I 3.3 x 10 9 g 1 CH 3 I CH 3 I 1% CH 3 I GC-ECD 125 I CH 3 I CH 3 I 14
16 Iodide oxidizing bacteria isolated from iodine-rich brine water - + IO - 3 I - I 2 I - 3 Marine Agar Difco 16S rdna Clustal W Ekho Lake Roseovarius tolerans 1 mm KCN NaN 3 EDTA unculturable culturable 15
17 Iodine accumulation onto soils and its mechanism I I I I I I 129 I 1600 I I I I I 125 II - IO I I % K d = / I 1000 I ICP- I I K d K d Br - Cl - I - - IO 3 I I IO I - I 0.1 g g -1 1 g g -1 I 30 g g -1 I I 16
18 Distribution and Behavior of Iodine in the Soil-Strata System and Soil Water- Groundwater System Actual Condition of the Experimental Fields (Paddy field, Upland field and Forest plot) ( 1 2 ) Yuita KouichiKihou NobuharuFujiwara Hideshi 50m 20,50,100,150,200,250cm ph ICP- MS mgkg -1 Apg 018cm 2.8 Bg cm 2Bw 6089cm Bwg 89120cm cm cm cm cm ph 17
19 L -1 20cm gl / cm cm 50cm cm cm /3 2/ Kd Kd 20cm Kd
20 Transfer of iodine from soil to vegetables - IAEA I 1600 kg 5g 2g 125 I 1/5000a NaI 1g * Muram atsu,y.et.al(1995)j.radioanal.nucl.chem.articles 194: Muram atsu,y.et.al (2002)Radioprotection 37 C1:
21 Enrichment of iodine content in edible portion of crops with the fertilizer containing iodine and a few problems KI 5000 a (1) (2) (3) KII20mg kg 2 3 ECD KI 0.12 mg kg mgkg mgkg 14 N. 20kg10a g mgkg Na 40gkg Na 30 gkg 60 2 t10a 112g 4 t10a 224g mgkg 4.9 mgkg 4 t10a mgkg 4.7 mgkg 2836 KI 46.1 mgkg 20
22 mgkg mgkg 22 N. 0.8 g mgkg mgkg 57.6 mgkg 20.6 mgkg KII20mgkg 14 N25kg10a 50 kg10a mgkg mgkg 0.5 mgkg Na N 6 kg10a 116 N40kg10a 2.9 mgkg N80kg10a mgkg I I0 3 I0 3 I I0 3 I NH4 NO 2 NO 3 NO 3 NO 2 NO N 2 O N 2 NO 3 NO 2 NH4 21
23 1.7GBq I
24 Monitoring of 129 I in the vicinity of Tokai Reprocessing Plant (Japan Nuclear Cycle Development Institute Tokai Works) Y UEZU, H WATANABE, M TAKEISHI and K SHINOHARA The reprocessing plant in Japan Nuclear Cycle Development Institute (JNC) Tokai Works is operating from The monitoring method of 129 I using neutron activation analysis, annual discharge of 129 I from reprocessing plant, concentrations of 129 I in soil, calculated values of 129 I in air and soil and the deposition velocity of 129 I will be described in this presentation.
25 129 Program for the measurement of iodine-129 at JAERI-Mutsu 129 I ( y) 129 Xe 235 U 129 I 129 I Sellafield La Hague 129 I 129 I 129 I I (AMS) 1997 HVEE AMS(model 4130) AMS 3MV 14 C 129 I 129 I 129 I 127 I (I 5+ ) 129 I 127 I I I (TOF-detector) ( 129 I/ 127 I) (7.497±0.119) (7.209±0.050) %0.7% AMS IO - 3 I - AgI 2 Sam pling Station 2 276sample( ) 238sample AMS AMS 24
26 Induction of rat thyroid cancers by radioactive iodine : Correlation between cancer induction and low-iodine uptake 131 Fisher 344 g 0.393, kbq 131 I g 1, 3 6 Gy Gy Total T 3,T 4 TSH 25
27 I 26
28 DIETARY IODINE INTAKE AND RADIATION PROTECTION Kunio SHIRAISHI : Department of Dose Assessment, Research Center for Radiation Emergency Medicine, National Institute of Radiological Sciences (KI) 10 g (ICP-MS) ICP-MS 0.2 ng/ml (ppb) mg % 1.57mg 1.77mg 1.77 g 1.3mg g 17220g/kg-(n=16) g /kg g /kg- n= g(n=67) 60g 38g 1,440 g g// g// 1.5mg 27
29 Iodine cycling from the Earth s mantle to thyroid 20g 40 I - ( IO - 3 ) 27 Muramatsu & Wedepohl ppm Missing-Link 129 I Muramatsu et al Muramatsu, Y., Fehn, U. and S. Yoshida: Recycling of iodine in fore-arc areas: evidence from the iodine brines in Chiba, Japan. Earth and Planetary Science Letters, 192, (2001) Muramatsu, Y. and Wedepohl, K.H.: The distribution of iodine in the earth's crust. Chemical Geology, 147, (1998). 28
30 Genesis of hydropressured natural gas and iodine deposits, and its points of issue 1/3 70% CO2 1 input output 1) 2) 3) 4) 1) 5) 29
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