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1 Discover Life Science Products and Services P2 4 P5 6 P7 11 PCRP11 12 P13 P14 15 P16 17 P18 19 P20 P20 Vol.2

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12 PCR TaqMan ID Applied Biosystems Applied Biosystems Applied Biosystems Step 1 ID TaqMan MicroRNA Assays TaqMan Gene Expression Assays TaqMan Array&Plate TaqMan SNP Genotyping Assays TaqMan Drug Metabolism Assays TaqMan Copy Number Assays TaqMan sirna Assays Silencer Select sirna Silencer sirna mirvana mirna mimics mirvana mirna inhibitors Ambion Pre-miR mirna Precursor Ambion Anti-miR mirna inhibitors Step Step 2 3 ID

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14 Lipofectamine mLLipofectamine 3000 Gibco Lipofectamine 3000 Reagent 1DNA,RNA, co-transfection 1.5 ml Lipofectamine 3000 Gibco Advanced DMEM 500mL Advanced DMEM/F mL TrypLE Express Enzyme 1100mL Opti-MEM I Reduced Serum Medium 500mL Opti-MEM I Reduced Serum Medium 500mL Lipofectamine 3000 Reagent 0.1 ml L , ml L , ml L , ml L , ml L , mLLipofectamine 3000 Advanced DMEM Advanced DMEM/F-12 TrypLE Express Enzyme1, no phenol red Opti-MEM I Reduced Serum Medium 500 ml 500 ml 100 ml 500 ml ,200 3,600 3,000 3,800 1 & DNA sirna mrna Lipofectamine 2000 DNA sirna DNAsiRNA Lipofectamine NEW 3000 DNA sirna DNAsiRNA Lipofectamine RNAiMAX sirna microrna NEW mrna Lipofectamine MessengerMAX Neon Transfection System DNA sirna microrna 0.1 ml L ml ml LMRNA001 TrypLE Express Advanced DMEM Enzyme MLDF22 MLBT22 Advanced DMEM/F Opti-MEM I Reduced Serum Medium 500mL MLUH22 MLEN22 14

15 Gibco B-27 mrna B-27 Lipofectamine MessengerMAX reagent B-27 mrnalipofectamine MessengerMAX reagent0.1 ml Lipofectamine MessengerMAX Reagent mrna DNA NEW DNA 1. Attachment to cell Reagent DNA complex 2. Endocytosis into cell Endosome 3. Escape from endosome DNA 4. Nuclear entry Nucleus Protein expression Protein Lipofectamine MessengerMAX mrna reagent DNA hnsc BJ RAW mrna Reagent mrna complex mrna mrna DNA vs mrna Lipofectamine MessengerMAX mrna mrnadna4 B-27 B-27 Supplement, Minus Insulin 10 ml A ,900 B-27 Supplement 50,custom B-27 Supplement Minus AO 50, Liquid B-27 Supplement Minus Vitamin A 50, Liquid B-27 Serum-Free Supplement 50, Liquid B-27 Electrophysiology Kit 10 ml 10 ml 10 ml 10 ml 1 kit SA A ,800 26,800 26,900 26,400 36,200 1 Lipofectamine MessengerMAX 0.1 ml B-27 Supplement, Xeno Free CTS 10 ml A ,100 Lipofectamine MessengerMAX reagent Lipofectamine MessengerMAX Reagent* ml LMRNA001 10, ml LMRNA003 29, ml LMRNA008 71, ml LMRNA ,100 Opti-MEM I Reduced Serum Medium 100 ml ,900 mmessage mmachine T7 ULTRA Transcription Kit* 2 10 AM ,200 * 1 mrna * 2 mrna MLBQ

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24 GeneArt CRISPR GeneArt CRISPR Nucrease Vector Kit CD4 OFP GeneArt Genomic Cleavage Detection Kit GeneArt Strings DNA Fragments GeneArt CRISPR / CRISPR! Facebook & Twitter facebook.com/lifetechnologiesjapan@lifetechjpn TaqMan is a registered trademark of Roche Molecular Systems, Inc. Used under permission and license. For Research Use Only. Not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Printed in Japan. LFT077-A1504OB TEL FAX TEL TEL

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