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1 pp. TFIIE TFIIH RNA II Pol II TFIIB TFIID TFIIE TFIIF TFIIH Pol II Pol II TFIIE α β TFIIE TFIIH DNA H A H B H H RNA II Pol II Pol II DNA pre-initiation complex PIC PIC Pol II TFIIB TFIID TFIIE TFIIF TFIIH Pol II TFIIB TFIID TFIIE TFIIF TFIIH Pol II TFIIB TFIID TFIIE TFIIF TFIIH Structural biology of a general transcription factor, TFIIE and its interaction mode with TFIIH Masahiko Okuda and Yoshifumi Nishimura Graduate School of Supramolecular Biology, Yokohama City University, Suehiro-cho, Tsurumi-ku, Yokohama, Japan PIC PIC TFIID TATA TATA-binding protein TBP
2 ５ ０ ２ 生化学 第８ ０巻 第６号 図１ 転写開始複合体 PIC の Stepwise assembly モデル 図２ PIC 構成因子の構造決定例 A DNA-TBP コアドメイン-TFIIB の C 末ドメイン複合体 PDB ID １VOL B Pol II 構造 完全１ ２サブユニット PDB ID １WCM C Pol II-TFIIB 複合体 PDB ID １R５U D 転写伸長中の Pol II の構造 PDB ID １I６H TBP のコアドメイン TFIIB の C 末ドメイン TFIIBc Pol II をリボンモデ ルで DNA RNA TFIIB の N 末ドメイン TFIIBn を球体モデルで示し ている 図の作製には PyMOL プログラム５７ を使用した
3 ２ ０ ０ ８年 ６月 ５ ０ ３ 図３ ヒト基本転写因子 TFIIE のドメイン構造 これまでに決定したドメイン構造 および配列から予想される構造モチーフ whth FH ウイングド ヘリックス ターン ヘ リックス フォークヘッド ZF ジンクフィンガー HTH ヘリックス ターン ヘリックス bhlh 塩基性ヘリックス ルー プ ヘリックス bhl 塩基性ヘリックス ループ STDE はセリン トレオニン アスパラギン酸 グルタミン酸に富んだ領域 Acidic は酸性アミノ酸に富んだ領域を示している 構造はリボンモデルで表示している H α ヘリックス S β 鎖 図の作製には MOLMOL プログラム５８ を使用した 図４ TFIIEαAC ドメインと TFIIH p６ ２ PH ドメインとの複合体の溶 液構造 A ２ ０個の NMR 主鎖構造の重ね合わせ PDB ID ２RNR B エ ネルギー最小構造のリボンモデル表示 図の作製には MOLMOL プ ログラム５８ を使用した
4 ５ ０ ４ 生化学 第８ ０巻 第６号 図５ 図６ 図７
5 Pol II PIC TFIIE TFIIE TFIIH TFIIE PIC Pol II TFIID TBP DNA DNA A TFIIB TFIIF Pol II TFIIE TFIIE TFIIH PIC DNA-TBP -TFIIB C Pol II Pol II-TFIIB PIC TFIIF Pol II TFIIE TFIIH TFIIH DNA TFIIE TFIIE α TFIIEα kda β TFIIEβ kda TFIIE α β TFIIE αβ PIC X TFIIE TFIIE TFIIF kda α kda β TFIIE TFIIE αβ NMR TFIIE TFIIE NMR TFIIEαAC TFIIEαAC AC TFIIH p PH AC N MOLMOL TFIIEαAC TFIIH p PH A TFIIEα AC N p PH B TFIIEα AC F V p PH C TFIIEα AC p PH A B AC PH GRASP PyMOL TFIIEαAC TFIIH p PH p TAD Tfb PH A TFIIEαAC -TFIIH p PH B p TAD -Tfb PH PDB ID GS P TfbB Tfb p MOLMOL
6 TFIIE TFIIEβ TFIIEβ C β whth FH DP HNF- γ DNA DNA DNA TFIIEβ DNA whth DNA TFIIEβ DNA NMR whth DNA TFIIE whth RFX whth DNA DNA TFIIEα TFE TFIIFα whth DNA TFIIFα whth CHD CHD Chd Chd Chd CHD H Chd CHD TFIIH DNA TFIIE DNA TFIIEβ DNA C bhl DNA TFIIEα TFIIEα C C C C C CD NMR N C DNA TFIIE TFIIH TFIIH TFIIE TFIIH PIC TFIIH Pol II kda TFIIH
7 XPB p p p p p TTDA CAK CDK activating kinase Cdk, cyclin H, MAT XPD TFIIH Pol II ATP DNA DNA ATP CTD Pol II Rpb C TFIIE ATP CTD PIC Pol II TFIIE TFIIH TFIIEα C AC TFIIE TFIIH TFIIEα C TFIIH TFIIEα TFIIH p TFIIEα C AC p p GST AC p N pleckstrin homology PH PH NMR TFIIEα AC NMR N C β α TFIIEα AC TFIIH p PH NOE p PH TFIIEα AC N β p PH AC N E E PH S S S β β A F A PH β S PH S S S S PH β B AC N N F F PH β B E E V F PH S H B AC N C AC N PH N TFIIE TFIIH p p TAD p TAD TAD TAD TAD p Tfb PH Tfb PH S S VP TAD Tfb p TAD VP TAD PH TFIIEα AC p TAD PH β TFIIEα AC N S TFIIEα AC F p TAD
8 F PH p TAD PH TFIIEα AC p TAD Tfb PH TFIIEα AC p PH TAD p TAD RPA A p TAD MDM VP TAD htafii TBP TFIIEα AC N p PH ITC p TAD p Tfb PH Kd nm nm NMR VP TAD Tfb PH Kd µm TFIIEα AC p PH Pol II TFIIH TFIIH TFIIE PIC p VP p E F- ERα p VP ERα p PH TFIIEα AC p PH TFIIE TAD p VP TFIIEα AC p PH TFIIE TFIIH PIC p TAD p PH TFIIH TFIIE TFIIH p TAD p PH p S T Kd nm S T Kd nm nm nm ITC TFIIEα p PH Kd nm TFIIEα p PH p TAD S T p TAD TFIIH p DNA p PH p DNA p TAD p TAD TFIIEα AC p S T p TAD p PH TFIIEα AC p p TFIIH DNA TFIIE αβ TFIIEβ C NMR X TFIIE NMR PIC TFIIE DNA Pol II TFIIEα AC TFIIH p PH TFIIE PIC
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Database Center for Life Science Online Service Masami Horikoshi, Ryosuke Himukai, Takashi Kuzuhara,
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pp. D- D-DAO DAO D- D- D- D- o- DAO-D- D-DAO DAO Hans Krebs FAD X DAO DAO DAO D- Structure and reaction mechanism of D-amino acid oxidase Yasuzo Nishina School of Health Sciences, Kumamoto University,
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pp. N Tom Tom Tom - Tom µm Soft interaction for the recognition of mitochondrial targeting signal Daisuke Kohda Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi,
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1 Department of Cancer and Thoracic Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Japan; 2 Department of Thoracic Surgery, Aichi Cancer Center, Japan;
Correlation between molecular evolution of P450s and evolution of plant secondary metabolism Masaharu Mizutani E-mail : mizutani@scl.kyoto-u.ac.jp URL : http://biofun.kuicr.kyoto-u.ac.jp/index-j.html 13)
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