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- えいじろう おおはし
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2 230700nm 1.5pg fluoresceinwell nm DEA activity unitswell Alkaline Phosphatase nm nm 3pg fluoresceinwell nm
3 nm UV 5 405,450,492,620nm nm 5 340,405,450,492,620nm 1nm nm nm nm,400700nm (19988) 1nm nm nm nm,400700nm 2 OD , nm 5 405,450,492,620nm
4 Code No. [] () s [GZT] IL-2, DuoseT, Human ,000 P [GZT] IL-4, DuoseT, Human ,000 P [GZT] IL-5, DuoseT, Human ,000 P [GZT] IL-6, DuoseT, Human ,000 P [GZT] IL-8, DuoseT, Human ,000 P [GZT] Total IL-12, DuoseT,Human ,000 P [GZT] IFN-, DuoseT, Human ,000 P [GZT] TGF-1, DuoseT, Human ,000 P [GZT] TNF-, DuoseT, Human ,000 P bfgf ELISA Kit Wako 60 70,000 OPD pgml 3pgml P * [GZT] Predicta GM-CSF ELISA Kit, Human 96 77,000 TMB pgml 2.5pgml * [GZT] Predicta IFN-, Human 96 77,000 TMB ,024pgml 3pgml * [GZT] Predicta IL-1, Human 96 77,000 TMB pgml 3pgml * [GZT] Predicta IL-2, Human 96 77,000 TMB ,024pgml 4pgml * [GZT] Predicta IL-4, Human 96 77,000 TMB ,024pgml 6pgml * IL-5 ELISA Kit Wako, Human 96 85,000 TMB ,000pgml 1pgml P * [GZT] Predicta IL-6, Human 96 77,000 TMB pgml 2pgml * [GZT] Predicta IL-8, Human 96 77,000 TMB pgml 1pgml * [GZT] Predicta IL-10, Human 96 77,000 TMB pgml 5pgml * [GZT] Predicta IL-12, Total, Human 96 77,000 TMB ,600pgml 10pgml P [GZT] Predicta IL-12, P70, Human 96 77,000 TMB pgml 2pgml P * [GZT] Predicta IL-15, Human 96 77,000 TMB ,500pgml 10pgml * [GZT] Predicta TNF-, Human 96 77,000 TMB ,024pgml 3pgml P * [GZT] Predicta TNF-R, Human 96 77, pgml 1pgml P * [GZT] Predicta TNF-R, Human 96 77, pgml 1pgml P * [GZT] Predicta TGF-1, Human 96 77,000 TMB ngml 0.05pgml s [GZT] IL-1, DuoseT, Mouse ,000 P [GZT] IL-2, DuoseT, Mouse ,000 P [GZT] IL-4, DuoseT, Mouse ,000 P [GZT] IL-10, DuoseT, Mouse ,000 P [GZT] IL-12 p70, DuoseT, Mouse ,000 P [GZT] Total IL-12, DuoseT, Mouse ,000 P [GZT] IFN-, DuoseT, Mouse ,000 P [GZT] TNF-, DuoseT, Mouse ,000 P [GZT] Inter Test IFN-, Mouse 96 77,000 TMB ,620pgml 5pgml [GZT] Inter Test IL-1, Mouse 96 77,000 TMB pgml 15pgml * [GZT] Inter Test IL-1, Mouse 96 77,000 TMB pgml 10pgml * [GZT] Inter Test IL-2, Mouse 96 77,000 TMB pgml 15pgml * [GZT] Inter Test IL-4, Mouse 96 77,000 TMB pgml 5pgml [GZT] Inter Test IL-6, Mouse 96 77,000 TMB pgml 5pgml * [GZT] Inter Test IL-10, Mouse 96 77,000 TMB ,080pgml 15pgml * [GZT] Inter Test IL-12 Total, Mouse 96 77,000 TMB ,860pgml 10pgml P * [GZT] Inter Test IL-12 P70, Mouse 96 77,000 TMB ,620pgml 5pgml P * [GZT] Factor Test TNF-, Mouse 96 77,000 TMB ,240pgml 15pgml P * [GZT] Factor Test TNF-R, Mouse 96 77, ,100pgml 15pgml P * [GZT] Factor Test TNF-R, Mouse 96 77, ,250pgml 12pgml P15
5 Code No. [] () s [] PanaTest 1-AG, Rat 96 96,000 OPD ngml P [] PanaTest Albumin, Rat 96 96,000 OPD ,000ngml P [] PanaTest Ferritin, Rat 96 96,000 OPD ngml P [] PanaTest Hemoglobin, Rat 96 96,000 OPD ngml P [] PanaTest IL-8, Rat 96 90,000 OPD ngml P [] PanaTest 2-Microglobulin, Rat 96 96,000 OPD ngml P [] PanaTest Prolactin, Rat ,000 OPD ngml P [] PanaTest Transferrin, Rat 96 96,000 OPD ngml P * [GZT] Factor Test TNF-, Rat 96 77,000 TMB ,240pgml 10pgml Rat IL-1 ELISA Kit Wako 96 70,000 TMB ,000pgml 3pgml P Rat IL-2 ELISA Kit Wako 96 70,000 TMB ,000pgml 10pgml P Rat IL-4 ELISA Kit Wako 96 70,000 TMB ,500pgml 15pgml P Rat IL-6 ELISA Kit Wako 96 70,000 TMB ,000pgml 3pgml P Rat IL-10 ELISA Kit Wako 96 70,000 TMB ,500pgml 20pgml P Rat TNF- ELISA Kit Wako 96 70,000 TMB ,000pgml 4pgml P Rat IFN- ELISA Kit Wako 96 70,000 TMB ,400pgml 13pgml P21 s [] ,000 OPD ngml P [] --T 96 45,000 TMB ngml P [] ,000 OPD ngml P [] --T 96 48,000 TMB ngml P [] --T 96 51,000 TMB ngml P [] --T 96 51,000 TMB ngml P22 [] --T 96 TMB ngml P22 genzyme-technep713 Code No. [] V : Visible, F : Fluoresence L : Luminescence s [MPI] RiboGreen RNA Quantitation Reagent 1ml 57,700 F RNA200pg P [MPI] RiboGreen RNA Quantitation Kit 1Kit 73,800 F RNA200pg P [MPI] OliGreen ssdna Quantitation Reagent 1ml 57,700 F ssdna1ngml P [MPI] OliGreen ssdna Quantitation Kit 1Kit 65,900 F ssdna1ngml P [MPI] PicoGreen dsdna Quantitation Reagent 1ml 49,700 F dsdna25pgml [MPI] PicoGreen dsdna Quantitation Kit 1Kit F dsdna25pgml * [ICA] Aurora Gal-XE Kit ,900 L 2fg20ng-Galactosidase P * [ICA] Aurora GUS Kit for Mammalian Cell ,400 L 0.06pg2ngBacterial - P * [ICA] Aurora GUS Kit for Plant Cell ,400 L Glucuronidase P * [ICA] Aurora AP Kit ,400 L 10fg1ngAlkaline Phosphatase P * ,000 L * LT ,000 L * LT ,000 L * ,000 L s [] NO2NO3 Assay Kit-F ,000 F NO110M P [] NO2NO3 Assay Kit-C ,000 V NOGriess10100M P26
6 s V : Visible, F : Fluoresence Code No. [] L : Luminescence s [] Cell Counting Kit 100 3,300 V [] Cell Counting Kit 500 9,800 V [] Cell Counting Kit 2,500 29,000 V [] Cell Counting Kit-F 100 4,800 F ,000 P [] Cell Counting Kit-F 500 9,800 F ,000 P [] Cell Counting Kit ,700 V ,0002,000 P [] Cell Counting Kit-8 2,500 31,000 V ,0002,000 P [MPI] CyQUANT Cell Proliferation Assay Kit ,900 F ,000 P Cytotoxic Fluoro-test Wako 1,000 P LDH-Cytotoxic Test Wako ,800 V 560 P [PCS] In VIVO CDK TM ELISA ,000 V [PCS] In VIVO PCNA TM ELISA ,000 V [] WST-1 25mg 7,000 V [] WST-1 100mg 15,900 V [] WST-1 500mg 53,500 V [ALA] alamar Blue TM 25ml 18,500 V P [ALA] alamar Blue TM 100ml 44,500 F P [] MTT 500 3,800 V ,0003, [] MTT 2,500 14,500 V ,0003, [SIG] XTT 100mg 17,500 V [MPI] EnzChek Protease Assay Kit green fluorescence ,900 F Uml [MPI] EnzChek DNase Solution Assay Kit 25 44,900 F DNase410-3 Kunitz250l [UBI] Protein Tyrosin Phosphatase Assay Kit ,000 V [UBI] Protein Tyrosin Phosphatase Assay Kit ,000 V [UBI] SerThr Phosphatase Assay Kit ,000 V [P-C] PKA Assay KitColorimetric ,100 F V : units [P-C] PKC Assay KitColorimetric ,100 F V : units [] GPDH , GPDH P34 s [P-C] BCA Protein Assay Reagent ,600 V ,000gml [P-C] Micro BCA Protein Assay Reagent ,400 V gml [P-C] Modified Lowry Protein Assay Reagent V Lowry [P-C] Coomassie Protein Assay Reagent 950ml 15,100 V 595 CBB11,500gml [P-C] Coomassie Plus Protein Assay Reagent 950ml 24,900 V 595 CBB11,500gml [MPI] NanoOrange Protein Quantitation Kit 1Kit 44,900 F ngml10gml [MPI] CBQCA Protein Quantitation Kit 1Kit F ngml150gml s [] ELISA 96 75,000 V pgml P * [] APE ELISA Kit 96 70,000 APE mglP * [] LAS ELISA Kit 96 50,000 LAS mglP [] ELISA 96 90,000 Vg7.8500ngmlP [] 8-OHdG Check 1set 80,000 DNA8-0HdG P Biopyrrin EIA Kit ,000 Biopyrrins P [MPI] Vybrant TM Multidrug Resistance Assay Kit 1Kit 47,900 F P31 * MPI : Molecular Probes Inc. ICA : ICN Pharmaceuticalcs, Inc. GZT : genzyme-techne ALA : Alamar UBI : Upstate Biotechnology, Inc. P-C : Pierce Chemical Co. PCS : Paracelsian SIG : Sigma
7 genzyme199871genzyme ELISATECHNE TECHNERD 2genzymeRD genzyme-techne lllllllllllllllllllllllllllllllllllllllllll s s
8 lllllllllllllllllllllllllllllllllllllllllll s s 2 1HRPH2O2 2 1,2- () QuantiGlo1pgml2,000pgml
9 ,000 CM <0.5pgml pgml 3.5h 200l S,P (C,E,H) 4.5h , ,000 CM <1pgml pgml 3.5h 200l S,P (C,E,H) 4.5h ,000 S,P (E) <0.1pgml pgml 5.5h 150l , ,000 CM <14pgml ,000pgml 4.5h 200l S,P (E,H) <22pgml ,000pgml ,000 CM <10pgml ,000pgml 3.5h 100l S,P (C,E,H) <20l ,000 CM,S,P (C,E,H) <7pgml ,000pgml 4.5h 100l ,000 CM,S,P (C,E,H) <6pgml ,000pgml 3.5h <20l ,000 CM,S,P (C,E,H) <7.4pgml ,000pgml 4.5h 200l , ,000 CM,S,P (C,E) <4.1pgml ,000pgml 4.5h 200l ,000 CM,S <0.09pgml pgml 6.5h 200l , ,000 CM,S,P (E,H) <5pgml ,000pgml 3.5h 50l ,000 CM,U <3pgml pgml 4.5h 200l S,P (C,E,H) 5.5h ,000 CM,S,P (C,E,H) <0.7pgml pgml 4.5h 100l , ,000 S,P (C,E),U <0.09pgml pgml 5.5h 200l ,000 CM,S,P (C,E,H) <0.2pgml 0.3-3,000pgml 5.5h 150l ,000 CM <3.5pgml ,000pgml 4.5h 100l S,P (C,E,H) <20l U 50l , ,000 CM <0.1pgml pgml 6.0h 200l S,P (C,E) pgml ,000 CM <10pgml ,000pgml 3.0h 50l S,P (C,E,H) 3.5h , ,000 CM <2pgml pgml 3.5h 20l S,P (C,E,H) 4.5h 200l ,000 S,P (C,E) <0.5pgml pgml 6.5h 200l ,000 CM <8pgml ,000pgml 5.0h <20l S,P (C,E,H) ,000pgml 4.5h 100l ,000 CM,S,P (C,E,H) <5pgml pgml 4.5h 200l U 3.5h ,000 CM <15pgml ,000pgml 3.5h 100l S,P (C,E,H) 4.5h ,000 CM <0.5pgml pgml 6.5h 200l S,P (E,H) pgml , ,000 CM,S,P (C,E,H) <32pgml ,000pgml 4.5h 100l
10 ,000 CM, S, P (E) <1pgml pgml 4.75h 50l ,000 P (H) 4.5h ,000 CM <16pgml ,000pgml 4.5h <20l S,P (C,E) 100l ,000 CM <3pgml ,000pgml 2.5h 200l S,P (C,E,H) <20l , ,000 CM <8pgml ,000pgml 3.5h 200l S,P (C,E,H) 4.5h ,000 CM <0.7pgml pgml 3.5h 200l S,P (C,E,H) 4.5h 200l U 3.5h <20l ,000 CM <15pgml ,000pgml 4.5h <20l S 25l P (C,E,H) 50l , ,000 CM <1pgml 5-320pgml 4.5h 200l S,P (C,E) pgml ,000 P (C,E) <0.28pgml pgml 5.5h 150l U <0.09pgml pgml ,000 CM,S,P (C,E,H) <30pgml ,000pgml 4.5h 100l , ,000 CM <7pgml 39-2,500pgml 3.5h 100l S,P (C,E,H) 4.5h ,000 CM <0.4pgml pgml 6.5h 200l S,P (E,H) <0.8pgml pgml 100l ,000 CM <2.8pgml pgml 3.5h 100l S,P (C,E,H) 4.5h ,000 S,P (E,H) <0.36pgml pgml 6.5h 150l U <0.26pgml pgml 200l ,000 CM <0.05ngml ngml 5.5h 100l S,P (C,E,H) <0.08ngml ngml 4.5h <20l ,000 CM <5pgml ,000pgml 2.75h 200l S,P (C,E,H) 4.5h ,000 CM <40pgml 125-8,000pgml 4.25h 50l S,P (C,E) 4.5h ,000 CM,S,P (C,E,H) <3pgml ,000pgml 5h 200l ,000 CM <15pgml ,000pgml 5.25h 100l S,P (C,E,H) 5.5h , ,000 CM <4pgml ,000pgml 3.5h 200l S,P (E,H) 4.5h ,000 CM <5pgml ,000pgml 3.5h 200l S,P (C,E,H) 4.5h 100l U 3.5h 100l
11 ,000 CM <9pgml ,000pgml 3.5h 50l S,P (C,E,H) <20l ,000 CM <6pgml ,000pgml 3.5h <20l S,P (C,E,H) <7pgml ,500pgml 4.5h 200l ,000 CM <4pgml ,000pgml 3.0h <20l S,P (C,E,H) <11pgml ,000pgml 4.5h 150l , ,000 CM <6pgml ,000pgml 3.5h 200l S,P (C,E,H) 4.5h ,000 CM <8.4pgml ,000pgml 4.5h 200l S <20l P (C,H) 20l ,000 CM <7pgml ,000pgml 3.5h 100l S,P (C,E,H) 4.5h ,000 CM <5pgml ,000pgml 3.5h 100l S <20l P (C,E,H) 25l U 50l ,000 CM,S,P (C,E,H) <4pgml ,000pgml 4.5h 100l ,000 CM <9pgml ,000pgml 4.5h 100l S,P (C,E,H) <20l U 20l ,000 CM <7pgml ,000pgml 5h 500l S,P (E) 100l ,000 CM,S,P (C,E,H) <7pgml ,000pgml 4.5h 125l ,000 CM <4.4pgml ,000pgml 3.5h 200l S,P (C,E,H) 4.5h ,000 S,P (C,E),U <0.18pgml pgml 6.5h 200l , ,000 CM <7pgml ,000pgml 4.5h 200l S,P (E,H) <16pgml ,000pgml ,000 CM <3pgml pgml 4.5h 200l S,P (C,E,H),U 20l ,000 CM <1pgml pgml 3.5h 200l S,P (C,E,H) 4.5h 20l U 3.5h 20l ,000 CM <15pgml ,000pgml 4.5h 200l S,P (C,E,H) ,000pgml ,000 CM <5pgml ,000pgml 4.5h 200l S,P (C,E,H) <9pgml ,000pgml 100l ,000 CM,S <3pgml pgml 4.5h 50l ,000 CM,S <3pgml ,000pgml 4.5h 50l , ,000 CM <2pgml pgml 4.5h 50l S <20l ,000 CM <3.1pgml ,000pgml 4.5h 50l S 25l
12 ,000 CM <4pgml ,000pgml 4.5h 50l S 25l ,000 CM,S <4pgml pgml 4.5h 50l , ,000 CM <1.5pgml pgml 4.5h 50l , ,000 CM,S <1pgml pgml 4.5h 50l ,000 CM,S <2pgml pgml 4.5h 50l ,000 CM <2pgml ,000pgml 4.5h 50l S 25l ,000 CM <10pgml ,000pgml 4.5h 50l S <20l , ,000 CM,S <1.5pgml pgml 4.5h 50l ,000 CM,S <1.5pgml pgml 4.5h 50l , ,000 CM,S <5.1pgml ,500pgml 4.5h 50l ,000 CM <20pgml ,000pgml 4.5h 50l S,P (C,E) <20l ,000 CM <3pgml pgml 4.5h 50l S <20l ,000 CM,S <5pgml ,000pgml 4.5h 50l , , B ,000 CM,S,P (C,E,H) <0.05ngml 0-5ngml 2.75h <20l B ,000 CM,S,P (C,E,H) <0.15ngml 0-14ngml 2.5h <20l B ,000 CM,S,P (E,H) <0.35ngml 0-46ngml 2.0h <20l B ,000 CM,S,P (H) <0.1ngml 0-10ngml 2.0h <20l B ,000 CM,S,P (C,E,H) <0.3ngml 0-59ngml 2.0h <20l B ,000 CM,S,P (C,E,H) <0.5ngml 0-46ngml 1.25h <20l B ,000 CM,S,P (E) <2ngml 0-76ngml 2.0h <20l
13 ,000 CM,Sa,U <30.1pgml ,000pgml 5.0h <20l ,000 CM <5.95pgml ,000pgml 2.75h 100l Sa,U <20l ,000 CM <36.2pgml 39-5,000pgml 2.75h 100l Sa,U <20l ,000 CM <1.4pgml 16-50,000pgml 2.75h 100l Sa,U <20l ,000 CM <4.62pgml ,000pgml 2.75h 100l Sa,U <20l ,000 CM,Sa,U <12.3pgml ,000pgml 2.75h <20l ,000 CM,Sa,U <18.8pgml ,000pgml 2.75h <20l ,000 CM,Sa,U <4.3pgml ,000pgml 3.0h 20l ,000 CM <7.98pgml ,000pgml 2.75h 100l Sa,U <20l ,000 CM,Sa,U <0.7pmolml 0.5-5,000pmolml 2.75h <20l ,000 CM <0.4pmolml 0.5-5,000pmolml 2.75h 100l Sa <20l ,000 CM,Sa,U <0.6pmolml pmolml 2.75h <20l ,000 CM,Sa,U <0.4pmolml pmolml 2.75h <20l ,000 CL,T NA 0-30mUl 3min <20l ,000 S,P (H) <2.5ngml ngml 5.5h <20l ,000 CL,T 5moll 0-100moll 10min l ,000 CL,T NA 0-8.4mUl 5min 200l ,000 CM,P (E),U 1ngml ngml 2.5h 100l ,000 P,T <0.1nmolml 0-20M 1.0h 200l ,000 CE,CM,P (E,H),U <1.5ngml ngml 4.5h <20l , , ,000 CL,P (H),T <0.2mUml 0-16mUml 1min 40l B ,000 P (Extracted) <1.0pgml 0-120pgml 1.5h 1ml , B ,000 P <0.42gml 0-13gml 2.5h <20l , ,000
14 12TH1TH2 IL-12p35p40 p35-p40 p40-p40 p40 (SDSp70) () () ( pgml ) p40p35 ngml IL-12 p70il-12total (Totalp70p40, p40) p70genetic Institute p70 IL pgml4,860 pgml ngml pgml 96195, , , rmouse IL-12 5g 44, Anti-Human IL-12, MAB p40, p70c mg 56, Anti-Human IL-12, MAB p40, p70c mg 56, Anti-Human IL-12, Rabbit 1ml 47, Anti-Human IL-12 p40p70, Biotinylated 0.55mg 62, Anti-Mouse IL-12, MAB p35red-t 0.5mg 56, Anti-Mouse IL-12, MAB p40c mg 56, Anti-Mouse IL-12, MAB p40c17.15, Biotinylated 0.5mg 62, Anti-Mouse IL-12, MAB p mg 56, Anti-Mouse IL-12, MAB p40c mg 56, Anti-Mouse IL-12 p40 p70, Biotinylated 0.55mg 62,000
15 TNFTNF (55kD) (75kD) , ,000 l l l l , ,000 l l l l g 58, g 58, g 62, g 62, g 62, g 62, wako 96 70, , , , ,000
16 mmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmm Q &A Q A Q A Q A Q A Q A Q A gl gl genzyme-techne Human 155,000set Mouse 15 Human 77,000kit96 Mouse 77,000kit96 Rat 77,000kit96 205,000set
17 IL-Interleukin- IL- ELISA IL- 826 IL- IL- ng 100ml ml IL- HRP ml TMBZDMF 0.5ml ml ml 1moll 20ml 10 l 3.91,000 pgml 4-parameter (R 2 1) pgml n 8 IL-pgml Mean SD CV n IL-pgml Mean SD CV IL-GM-CSF IL-TNF-IFNIL Human IL-5 ELISA Kit Wako 96 85,000 Fukuda, Y., Hashino, J., Haruyama, M., Tsuruoka, N., Nakazato, H.and Nakanishi, T. : J. Immunol. Methods,143,891991
18 bfgf 5500pgml 3pgml bfgf No.5298 bfgfbfgf HRP bfgf No. 3H3Fab' ng 30ml 15ml HRP 17ml OPD ml 1moll 12ml pgml ( 50l) 3pgml ( 50l) 5500pgml 0.99(3-D Log-Logn=3) l l l bfgf ELISA Kit Wako 60 70,000
19 () () (IL-1, IL-4, IL-6, IL-10, TNF-, IFN-, IL-2) () 30ml () 7ml12ml () HRP (100) 0.15ml l l () HRP 15ml () (TMBz) 12ml () (25) 50ml () 12ml (10) IL-1IL-1 IL-1IL-3 IL-5IL-6IL-7IL-8IL-10IL-12IL-13 GM-CSFIFN-IL-2IL-6IL-10 IFN-TNF-TNF-MIP-2 MCP-1 l l l IL-4 IL-1IL-2 IL-3IL-4IL-5IL-7IL-8IL-10IL-12 IL-15IL-2IL-3IL-4IL-6IFN- TNF-MCP-1MIP-2TNF- l l l
20 IL-6 IL-1IL-2IL-6IL-7IL-8IL-13IL-15 IFN-IL-1IL-2IL-10MCP-1 TNF-IL-1IL-4IL-10TNF- MIP-2 l l l IL-10IL-1IL- 7IL-8IL-10IL-15IFN-GM-CSF RANTESTNF-IL-1IL-2IL- 3IL-4IL-6IL-10TNF-IL-1 IL-4TNF-IFN-MIP-2 l l l TNF- TNF-IL-1, IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-13, IFN-, TNF-IL-1, IL-2, IL-4, IL-6, IL-10, IFN- IFN-, MIP-2 l
21 l l IFN- IFN- IFN-IFN- l l l IL-2IL- 2, IL-7, IL-8, IL-10, IL-15, GM-CSF, IFN- IL-1, IL-2, IL-3, IL-4, IL-6, IL-10, MCP-1, TNF-IL-1, IL- 4, IFN-, MIP-2, TNF- l l l
22 () (10l) () ngml ngml 25l 3. 60ml 4. 5l l 6. TMB 12ml 7. 12ml 8. (1moll H2SO4) 12ml 9. (10) 50ml OPDTMB AKRIN , AKRIN-010T --T 96 45, AKRIN , AKRIN-011T --T 96 48, AKRIN-012T --T 96 51, AKRIN-013T --T 96 51,000 2 AKRIN-014T --T 96 1OPD21 kkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkkk EIA () PRA011 PRB021 PRC031 PRD051 PRE061 PRF081 PRG091 PRH IL-8* 2-96, ,000 96,000 96,000 96,000 90,000 96,000 96, *IL ng/ml ng/ml 5-160ng/ml 16-1,000ng/ml ng/ml ng/ml ng/ml ng/ml
23 l 200A260 RiboGreen TM RNA Quantitation Reagent 1ml 1,000 20TE 25ml 1ngml1gml Ribosomal RNA Standard (100gml) 5200l ATP ex480nmem520nm 200l 200pg RiboGreen TM DNA DNaseRNA l 2ml100pgml ssdna A26010, pgml1gml ATP DNARNA OliGreen ssdna Quantitation Reagent 20TE Oligonucleotide Standard (100gml) 200 1ml 25ml 1ml 200l 1ngml l l l
24 2,3 -Galactosidase O-nitrophenyl--D-galactoside(ONPG)1,000() CAT1,000 ( 2fg20ng-Galactosidase 1 ) 4,000-Galactosidase GalactoLux Chemiluminescent Substrate (100) 400l 1.2ml Lysis Solution 70ml 210ml (100mM Potassium Phosphate ph 7.8, 0.2% Triton X-100) Reaction Buffer Diluent 40ml 120ml (100mM Sodium Phosphate ph 8.0, 1mM MgCl2) Light Emission Accelerator 70ml 210ml GalactoLux Chemiluminescent Substrate DTT DTT Accelerator10mM H2O2 -galactosidaselysis SolutionpH 4850Lysis Solution5mg/ml PMSFAEBSF4860 PMSF1,800/1g ( ), 6,000/5g ( ), 18,900/25g ( ) AEBSF (p-absf)7,800/100mg ( ), 56,000/1g ( ) Bacterial -Glucuronidase 4-methylumbelliferyl--D-glucuronide(MUG) 100() 0.06pg2ngBacterial-Glucuronidase
25 GusLux Chemiluminescent Substrate ( l 1.2ml Lysis Solution 70ml 210ml ( : 50mM Potassium Phosphate ph 7.0, 10mM EDTA, 0.1% SDS, 0.1% Triton X-100) ( : 100mM Potassium Phosphate ph 7.8, 0.2Triton X-100) Reaction Buffer Diluent 40ml 120ml (100mM Sodium Phosphate ph 7.0, 10mM EDTA) Light Emission Accelerator 70ml 210ml Secreted Alkaline Phosphatase CAThGH1,000(10fg1ng ) BufferAlkaline Phosphatase StarLight Chemiluminescent Substrate 1.0ml 3.0ml Assay Buffer (Differential alkaline Phosphatase Inhibitor) 20ml 60ml Reaction Buffer Diluent (Luminescence Enhancer) 19ml 57ml 5Dilution Buffer 5ml 15ml Contorol Enzyme 50l 50l Aurora Gal-XE Kit Aurora GUS Kit for Plant Cell Aurora GUS Kit for Mammalian Cell Aurora AP Kit ,900 74,600 35,400 88,000 35,400 88,000 35,400 90,600 1) Jain,V.and Magrath, I.: Anal.Biochem., 199,119 (1991) 2) Fulton, R. and Van Ness, B.: Bio Techniques., 14,762 (1993) 3) Nguyen,V.T.,Morange,M.and Bensaude, O. : Anal.Biochem.,171,404 (1988)
26 NH 2 NH 2 2,3-diaminonaphthalene N H N N naphthalenetriazole 2,500 2,000 1,500 1, µm) (λem=450nm, λex=365nm)
27 LDH () (3.7mgvial) NAD 5ml10 DL- (50mgml) 55ml (1moll) 55ml 10 () MTT l alamar Blue 25ml 100ml 18,500 44,500
28 DCalcein-AM D D DHeLaHL605025,000 D100 Calcein-AM in DMSO PBS( ) Cell Counting Kit-FDMSOPBS () 50 ( ) 10l 30 (ex=485nm,em=535nm) Cell Counting Kit-8 WST-8 [2- (2-methoxy-4- nitrophenyl)-3- (4-nitrophenyl) -5- (2, 4-disulfophenyl)- 2H-tetrazolium, monosodium salt] ( ) Cell Counting Kit 5ml mm WST-8 0.2mM 1-Methoxy PMS 150mM NaCl 450nm
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36 zzzzzzzzzz zzzzzzzzzz 10 ( APE) ( ) APE APE () (12- ) APE ( mg ) APENP10EO, 1mg/l 20% 4ml 1 7ml 2 8ml 2 250l 1 15ml 1 APE 96well 1 96well ml 1 15ml 1 AAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA ,2-2 WAKO BIO WINDOW OCT APE ELISA , APE ELISA ,000 ggggggg ggggggg mg/l 2 LAS : linear - Alkylbenzene Sulfonate LAS ELISA , LAS ELISA , pp ) 48pp ) Fujita, et al.: Environ. Sci. Technol., 32,
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